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https://www.readbyqxmd.com/read/28330303/bio-prospecting-of-cuttle-fish-waste-and-cow-dung-for-the-production-of-fibrinolytic-enzyme-from-bacillus-cereus-ind5-in-solid-state-fermentation
#1
Gurupatham Devadhasan Biji, Arumugaperumal Arun, Eswaran Muthulakshmi, Ponnuswamy Vijayaraghavan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi
The process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing Bacillus cereus IND5 and using cuttle fish waste and cow dung substrate were optimized. The pH value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and pH, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. Central composite design was applied to investigate the interactive effect among variables (pH, casein and magnesium sulfate) and response surface plots were created to find the pinnacle of process response...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28321408/novel-sequential-screening-and-enhanced-production-of-fibrinolytic-enzyme-by-bacillus-sp-ind12-using-response-surface-methodology-in-solid-state-fermentation
#2
Ponnuswamy Vijayaraghavan, P Rajendran, Samuel Gnana Prakash Vincent, Arumugaperumal Arun, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Oh Young Kwon, Young Ock Kim
Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28264513/influence-of-indole-3-acetic-acid-and-gibberellic-acid-on-phenylpropanoid-accumulation-in-common-buckwheat-fagopyrum-esculentum-moench-sprouts
#3
Chang Ha Park, Hyeon Ji Yeo, Yun Ji Park, Abubaker M A Morgan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Sang Un Park
We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS) analysis. Among these, seven compounds (4-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and quercetin) were quantified by high-performance liquid chromatography (HPLC) after treating the common buckwheat sprouts with different concentrations of the hormones IAA and GA...
February 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28218705/accumulation-of-carotenoids-and-metabolic-profiling-in-different-cultivars-of-tagetes-flowers
#4
Yun Ji Park, Soo-Yun Park, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Hyung-Geun Ahn, Jae Kwang Kim, Sang Un Park
Species of Tagetes, which belong to the family Asteraceae show different characteristics including, bloom size, shape, and color; plant size; and leaf shape. In this study, we determined the differences in primary metabolites and carotenoid yields among six cultivars from two Tagetes species, T. erecta and T. patula. In total, we detected seven carotenoids in the examined cultivars: violaxanthin, lutein, zeaxanthin, α-carotene, β-carotene, 9-cis-β-carotene, and 13-cis-β-carotene. In all the cultivars, lutein was the most abundant carotenoid...
February 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28149185/promoter-polymorphism-590-t-c-of-interleukin-4-il4-gene-is-associated-with-rheumatoid-arthritis-an-updated-meta-analysis
#5
REVIEW
Hyun Kyung Park, Su Kang Kim, Hae Yong Kweon, Kwan Gill Lee, Mariadhas Valan Arasu, Young Ock Kim
Rheumatoid arthritis (RA) is a chronic disease. It causes chronic inflammation of the joint. Recent studies suggested that interleukin 4 (IL4) contributes to susceptibility and severity of rheumatoid arthritis (RA). Especially, it was reported that promoter polymorphism (-590, T/C) of IL4 gene has been associated with susceptibility of RA. The aim of present study was to investigate whether the promoter polymorphism (-590, T/C) of IL4 gene is associated with the susceptibility of RA using meta-analysis. And in order to perform meta-analysis, comprehensive meta analysis program was used...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28149184/combined-effect-of-nitrogen-phosphorus-and-potassium-fertilizers-on-the-contents-of-glucosinolates-in-rocket-salad-eruca-sativa-mill
#6
Jin-Hyuk Chun, Silbia Kim, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Doug Young Chung, Sun-Ju Kim
Nitrogen (N), phosphorous (P) and potassium (K) are the most limiting factors in crop production. N often affects the amino acid composition of protein and in turn its nutritional quality. In Brassica plants, abundant supply of N fertilizer decreases the relative proportion of glucosinolates (GSLs), thus reducing the biological and medical values of the vegetables. Hence effort was made to evaluate the influence of different proportions of nutrient solutions containing N-P-K on the GSL profiles of rocket salad (Eruca sativa Mill...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28126555/bactericidal-activity-of-fish-galectin-4-derived-membrane-binding-peptide-tagged-with-oligotryptophan
#7
Abirami Arasu, Venkatesh Kumaresan, Munuswamy-Ramanujam Ganesh, Mukesh Pasupuleti, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Jesu Arockiaraj
Galectins belong to the family of galactoside-binding proteins which act as pathogen recognition receptors by recognizing and binding to the carbohydrate present in the bacterial membranes. In this study, a Galectin-4 sequence was identified from the constructed cDNA library of Channa striatus and its structural features were reported. Gene expression analysis revealed that CsGal4 was highly expressed in liver and strongly induced by Epizootic Ulcerative Syndrome (EUS) causing pathogens such as Aphanomyces invadans, Aeromonas hydrophila and a viral analogue, poly I:C...
January 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28059104/synthesis-and-insecticidal-activity-of-acridone-derivatives-to-aedes-aegypti-and-culex-quinquefasciatus-larvae-and-non-target-aquatic-species
#8
Selvaraj Mohana Roopan, Annadurai Bharathi, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, G Madhumitha
A serious Mosquito borne yellow fever is one of the grave diseases which affect the major population. Since there is no specific treatment for yellow fever, there is a necessity to develop an effective agent. The series of acridinone analogues 3 to 5 were synthesized with help of non-conventional microwave heating and confirmed by respective spectral characterization. 5c and 3b showed highest activity to kill 90% of larvae against A. aegypti and C. quinquefasciatus, respectively. Also the active products were treated to check the mortality of non-target aquatic species...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28036069/ginsenoside-re-promotes-osteoblast-differentiation-in-mouse-osteoblast-precursor-mc3t3-e1-cells-and-a-zebrafish-model
#9
Hye-Min Kim, Dong Hyun Kim, Ho-Jin Han, Chan-Mi Park, Srinivas Rao Ganipisetti, Mariadhas Valan Arasu, Young Ock Kim, Chun Geun Park, Bo-Yeon Kim, Nak-Kyun Soung
Bone homeostasis is tightly regulated to balance bone formation and bone resorption. Many anabolic drugs are used as bone-targeted therapeutic agents for the promotion of osteoblast-mediated bone formation or inhibition of osteoclast-mediated bone resorption. Previous studies showed that ginsenoside Re has the effect of the suppression of osteoclast differentiation in mouse bone-marrow derived macrophages and zebrafish. Herein, we investigated whether ginsenoside Re affects osteoblast differentiation and mineralization in in vitro and in vivo models...
December 29, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27933594/vegetable-peel-waste-for-the-production-of-zno-nanoparticles-and-its-toxicological-efficiency-antifungal-hemolytic-and-antibacterial-activities
#10
T V Surendra, Selvaraj Mohana Roopan, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Gargi Sarkar, K Suthindhiran
Zinc oxide (ZnO) nanoparticles (NPs) are important materials when making different products like sun screens, textiles, and paints. In the current study, the photocatalytic effect of prepared ZnO NPs from Moringa oleifera (M. oleifera) was evaluated on degradation of crystal violet (CV) dye, which is largely released from textile industries and is harmful to the environment. Preliminarily, ZnO NP formation was confirmed using a double beam ultraviolet visible (UV-Vis) spectrophotometer; further, the NP size was estimated using XRD analysis and the functional group analysis was determined using Fourier transform infrared (FT-IR) spectroscopy...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27908201/cocos-nucifera-coir-mediated-green-synthesis-of-pd-nps-and-its-investigation-against-larvae-and-agricultural-pest
#11
Ganesh Elango, Selvaraj Mohana Roopan, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Kasinathan Irukatla Damodharan, Kuppuswamy Elumalai
In recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. Cocos nucifera (C. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. Cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help C. nucifera coir, Palladium nanoparticles (Pd NPs) were synthesized. Green-synthesized spherical-shape Pd NPs were over layered by secondary metabolites from C...
December 1, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27876624/differences-in-structure-and-changes-in-gene-regulation-of-murrel-molecular-chaperone-hsp-family-during-epizootic-ulcerative-syndrome-eus-infection
#12
Akila Sathyamoorthy, Mukesh Kumar Chaurasia, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ramasamy Harikrishnan, Jesu Arockiaraj
Heat shock proteins (HSPs) are immunogenic, ubiquitous class of molecular chaperones, which are induced in response to various environmental and microbial stressful conditions. It plays a vital role in maintaining cellular protein homeostasis in eukaryotic cells. In this study, we described a comprehensive comparative data by bioinformatics approach on three different full length cDNA sequences of HSP family at molecular level. The cDNA sequences of three HSPs were identified from constructed cDNA library of Channa striatus and named as CsCPN60, CsHSP60 and CsHSP70...
January 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27869743/a-metal-free-regioselective-multicomponent-approach-for-the-synthesis-of-free-radical-scavenging-pyrimido-fused-indazoles-and-their-fluorescence-studies
#13
Jeyakannu Palaniraja, Selvaraj Mohana Roopan, G Mokesh Rayalu, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu
This study deals with a new and efficient metal-free regioselective synthesis of pyrimido-fused indazoles with nitrogen ring junction motifs. We have developed a metal-free domino type reaction between 3-aminoindazole, aryl aldehydes and aceotophenones in the presence of KOH/DMF that leads to pyrimido[1,2-b]indazole analogues. Response Surface Methodology (RSM) coupled with a Box-Behnken design (BBD) were utilized for exploring the effect of base used (A), temperature of reaction (B) and (C), reaction time...
November 18, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27827861/lc-ms-ms-validation-analysis-of-trastuzumab-using-dsil-approach-for-evaluating-pharmacokinetics
#14
Rohit H Budhraja, Milin A Shah, Mahendra Suthar, Arun Yadav, Sahil P Shah, Prashant Kale, Parisa Asvadi, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Chun Geon Park, Young-Ock Kim, Hak Jae Kim, Y K Agrawal, Ravi K Krovidi
Quantitative targeted proteomics based approaches deploy state-of-the-art Liquid chromatography tandem mass spectrometry LC-MS technologies and are evolving as a complementary technique to standard ligand-binding based assays. Advancements in MS technology, which have augmented the specificity, selectivity and sensitivity limits of detection and freedom from antibody generation, have made it amicable towards various clinical applications. In our current work, a surrogate peptide based quantitative proteomics assessment is performed by selecting specific signature peptides from the complementary determining region CDR region of trastuzumab (Herclon(®), Roche products in India)...
November 2, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27795886/biological-potency-and-characterization-of-antibacterial-substances-produced-by-lactobacillus-pentosus-isolated-from-hentak-a-fermented-fish-product-of-north-east-india
#15
Chirom Aarti, Ameer Khusro, Mariadhas Valan Arasu, Paul Agastian, Naïf Abdullah Al-Dhabi
Lactic acid bacteria (LAB) isolated from various foods are important due to their potential to inhibit microorganisms, including drug-resistant bacteria. The objectives of this investigation were to isolate and identify antibacterial substances producing LAB from Hentak, a traditional fermented fish product of Manipur (North-East India), and to optimize the production of antagonistic substances present in cell free neutralized supernatant (CFNS) against enteric bacterial pathogens using the 'one factor at a time' (OFAT) method...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27729229/bacterial-membrane-binding-and-pore-formation-abilities-of-carbohydrate-recognition-domain-of-fish-lectin
#16
Abirami Arasu, Venkatesh Kumaresan, Rajesh Palanisamy, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Munuswamy-Ramanujam Ganesh, Jesu Arockiaraj
Antimicrobial peptides (AMPs) are innate molecules that are found in a wide variety of species ranging from bacteria to humans. In recent years, excessive usage of antibiotics resulted in development of multi-drug resistant pathogens which made researchers to focus on AMPs as potential substitute for antibiotics. Lily type mannose-binding lectin is an extended super-family of structurally and evolutionarily related sugar binding proteins. These lectins are well-known AMPs which play important roles in fish defense mechanism...
February 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27722047/composition-of-volatile-compounds-and-in-vitro-antimicrobial-activity-of-nine-mentha-spp
#17
Yun Ji Park, Thanislas Bastin Baskar, Sun Kyung Yeo, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Soon Sung Lim, Sang Un Park
BACKGROUND: Mentha plants containing over 25 species are aromatic perennial herbs. These species have been interested and widely used because of various clinical findings. Many volatile compounds facilitate environmental interactions such as protecting themselves from pathogens, parasites, and herbivores. Therefore, this study assessed comparison of volatile composition and antimicrobial activity from nine Mentha species. The composition of volatiles was investigated from the aerial parts of nine different Mentha species using gas chromatography-mass spectrometry (GC/MS)...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27672449/mechanochemical-synthesis-of-chitosan-submicron-particles-from-the-gladius-of-todarodes-pacificus
#18
J R Anusha, Albin T Fleming, M Valan Arasu, Byung Chul Kim, Naif Abdullah Al-Dhabi, Kook-Hyun Yu, C Justin Raj
The present work focused on the synthesis of β-chitosan submicron particles (CSPs) from Todarodes pacificus using mechanochemical techniques. The gladius was submitted to a sequence of mechanical and chemical treatments to synthesize β-chitin (CT), which was further deacetylated to form spherical chitosan submicron particles with an average diameter of ⩽100 nm. The surface morphology of β-chitin and CSPs was observed using electron microscopy. The degree of deacetylation (DD%), evaluated from the absorbance peak of a Fourier Transform Infrared (FTIR) spectrum, was 80 ± 2...
November 2016: Journal of Advanced Research
https://www.readbyqxmd.com/read/27648859/pellino-1-derived-cationic-antimicrobial-prawn-peptide-bactericidal-activity-toxicity-and-mode-of-action
#19
Gayathri Ravichandran, Venkatesh Kumaresan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Munuswamy-Ramanujam Ganesh, Arun Mahesh, Arunkumar Dhayalan, Mukesh Pasupuleti, Jesu Arockiaraj
The antimicrobial peptides (AMPs) are multifunctional molecules which represent significant roles in the innate immune system. These molecules have been well known for decades because of their role as natural antibiotics in both invertebrates and vertebrates. The development of multiple drug resistance against conventional antibiotics brought a greater focus on AMPs in recent years. The cationic peptides, in particular, proven as host defense peptides and are considered as effectors of innate immunity. Among the various innate immune molecules, functions of pellino-1 (Peli-1) have been recently studied for its remarkable role in specific immune functions...
October 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27615833/redescriptions-and-new-host-records-of-chewing-lice-of-the-genus-ricinus%C3%A2-phthiraptera-ricinidae-from-the-neotropical-region
#20
Miroslav Valan, Oldrich Sychra, Ivan Literak
Two species of the chewing louse genus Ricinus are redescribed and illustrated: Ricinus dalgleishi Nelson, 1972 from Helmitheros vermivorum (Gmelin, 1789), a new host-louse association, and Ricinus tanagraephilus Eichler, 1956 from Euphonia laniirostris d'Orbigny & Lafresnaye, 1837. Also, new host-louse associations are recorded for Ricinus vireoensis Nelson, 1972 from Vireo pallens Salvin, 1863, and for females of an unidentified species of Ricinus sp. from Corythopis delalandi (Lesson, 1831), which are described and illustrated...
August 19, 2016: Zootaxa
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