Read by QxMD icon Read


Fang Hu, Bin Liu
Bioprobes based on fluorogens with aggregation-induced emission (AIE) characteristics have been increasingly used in chemosensing and bioimaging due to their high sensitivity, photostability and biocompatibility. In this review, we summarize the design of cellular organelle specific (cytoplasm membrane, mitochondria, lysosomes, lipid droplets and nucleus) AIE bioprobes and their applications in organelle imaging, organelle bioactivity monitoring, and image-guided cancer cell ablation.
October 25, 2016: Organic & Biomolecular Chemistry
Amanda N Fletcher, Agostino Molteni, Rakesh Ponnapureddy, Chirag Patel, Mark Pluym, Alan M Poisner
BACKGROUND: Fat embolism (FE) and the consequent fat embolism syndrome (FES) occurring after trauma or surgery can lead to serious pulmonary injury, including ARDS and death. Current treatment of FES is limited to supportive therapy. We have shown in a rat model that the renin angiotensin system (RAS) plays a significant role in the pathophysiology of FE since drugs interfering with the RAS, captopril and losartan, reduce the histopathologic pulmonary damage. The purpose of the current study was to determine if inhibition of renin by aliskiren, an FDA-approved drug for treating hypertension, would produce effective protection in the same model...
October 25, 2016: Journal of Trauma and Acute Care Surgery
Wei Lu, Alan L Kelly, Pierce Maguire, Hongzhou Zhang, C Stanton, Song Miao
In this study, an in vitro Caco-2 cell culture assay was employed to evaluate the correlation between emulsion structure and cellular uptake of encapsulated β-carotene. After 4h incubation, an emulsion stabilized with whey protein isolate showed the highest intracellular accumulation of β-carotene (1.06 μg), followed by that stabilised with sodium caseinate (0.60 μg) and Tween® 80 (0.20 μg), which are 13-, 7.5-, and 2.5-fold higher than that of free β-carotene (0.08 μg), respectively. Emulsions with small droplet size (239±5 nm) showed a higher cellular uptake of β-carotene (1...
October 25, 2016: Journal of Agricultural and Food Chemistry
Annie Gravel, Isabelle Dubuc, Angela Brooks-Wilson, Kristan J Aronson, Jacques Simard, Hector Velasquez-Garcia, John J Spinelli, Louis Flamand
Background Inherited chromosomally-integrated human herpesvirus 6 (iciHHV-6) is a condition observed in approximately 1% of the population. Whether such a genetic alteration predisposes to cancer development in currently unknown. Two studies were conducted to determine whether iciHHV-6 is associated with cancer development. Methods First, a screen of 19,597 people from the province of Quebec was conducted. A replication test, using data from a population-based case-control study of 1090 women with incident breast cancer and 1053 controls from British Columbia and Ontario was conducted...
October 24, 2016: Cancer Epidemiology, Biomarkers & Prevention
Yadong Yu, Tao Li, Na Wu, Lujing Ren, Ling Jiang, Xiao-Jun Ji, He Huang
Arachidonic acid (ARA) is an important polyunsaturated fatty acid, which has various beneficial physiological effects on the human body. The aging of Mortierella alpina (M. alpina) has long been known to significantly improve ARA yield, but the exact mechanism is still elusive. Herein, multiple approaches including large-scale comparative proteomics were employed to systematically investigate the mechanism mentioned above. Upon ultrastructural observation, abnormal mitochondria were found to aggregate around shrunken lipid droplets...
October 25, 2016: Journal of Agricultural and Food Chemistry
Sanna Björkegren, Lars Nordstierna, Anders Törncrona, Anders Palmqvist
Colloidal silica particles, functionalized with hydrophilic and hydrophobic groups, have been studied for utilization in particle-stabilized emulsions, so called Pickering emulsions. The amounts of attached groups have been characterized using NMR spectroscopy and elemental analysis. A range of particles were prepared, with sizes from around 13 to 70nm in diameter. Hydrophilic functionalization of the silica sols was achieved by attaching methyl poly(ethylene glycol) (mPEG) silane to the silica particle surface...
October 15, 2016: Journal of Colloid and Interface Science
Ingo Hoffmann, Miriam Simon, Anja Hörmann, Thorsten Gravert, Peggy Heunemann, Sarah E Rogers, Michael Gradzielski
Nanoemulsions are metastable emulsions with droplet sizes between 20 and 100 nm with a wide range of applications e.g. in polymerization, in pharmaceutical and cos- metic formulations and as drug delivery systems. Even though they are not in ther- modynamic equilibrium, they can be metastable over relatively long times and have the advantage that they can be formed easily by low energy input methods. In par- ticular, the phase inversion concentration method (PIC) allows to form nanoemulsions simply by diluting a suitable mixture of oil and surfactants with water...
October 24, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Paul S Sheeran, Naomi Matsuura, Mark A Borden, Ross Williams, Terry O Matsunaga, Peter N Burns, Paul A Dayton
Continued advances in the field of ultrasound and ultrasound contrast agents have created new approaches to imaging and medical intervention. Phase-shift perfluorocarbon droplets, which can be vaporized by ultrasound energy to transition from the liquid to the vapor state, are one of the most highly researched alternatives to clinical ultrasound contrast agents (i.e. microbubbles). In this review, part of a special issue on methods in biomedical ultrasonics, we survey current techniques to prepare ultrasound-activated nanoscale phase-shift perfluorocarbon droplets, including sonication, extrusion, homogenization, microfluidics, and microbubble condensation...
October 20, 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Yankai Jia, Yukun Ren, Weiyu Liu, Likai Hou, Ye Tao, Qingming Hu, Hongyuan Jiang
Correction for 'Electrocoalescence of paired droplets encapsulated in double-emulsion drops' by Yankan Jia et al., Lab Chip, 2016, DOI: .
October 24, 2016: Lab on a Chip
Jiangping Xu, Jun Li, Yi Yang, Ke Wang, Nan Xu, Jingyi Li, Ruijing Liang, Lei Shen, Xiaolin Xie, Juan Tao, Jintao Zhu
Although high-boiling non-solvent induced macrophase separation in emulsion droplets has been widely applied for the fabrication of polymeric capsules, precise control of their structures remains a great challenge. Herein, block copolymer capsules with tunable shell structures were fabricated by employing a non-solvent as a liquid template in emulsion droplets. The properties of the non-solvents dictate the phase separation sequence in the droplets and the capsule formation mechanism. Two different pathways for capsule formation were observed, and could be applied to predict the shell structure...
October 24, 2016: Angewandte Chemie
Yau Kei Chan, Gabriela Czanner, Ho Cheung Shum, Rachel L Williams, Ning Cheung, David Wong
PURPOSE: Emulsification is related to complications arising from silicone oil (SO) tamponade. Currently, there is no widely accepted method for testing the propensity of SO to emulsify that are physiologically realistic and quantitative. METHODS: We compared different ways of inducing emulsification namely vortex mixing, sonication and homogenization. Silicone oil (SO) emulsification was quantitatively assessed using the Coulter counter and laser light scattering...
October 24, 2016: Acta Ophthalmologica
Yosuke Iwai, Yoshiaki Uchida, Hiroshi Yabu, Norikazu Nishiyama
Microfluidic devices, which can continuously fabricate single emulsion with monodispersed droplets having a pore diameter of more than 100 μm in large numbers, can be applied to manufacture ordered macroporous films. 3D ordered macroporous films with a diameter of more than 100 μm can be fabricated using ordered arrays of the monodispersed droplets as templates of the macropores, which are self-assembled in the space between two parallel flat glass plates. As the gap between the glass plates increases, the number of the layer increases...
October 24, 2016: Macromolecular Rapid Communications
Zitian Chen, Yusi Fu, Fangli Zhang, Lu Liu, Naiqing Zhang, Dong Zhou, Junrui Yang, Yuhong Pang, Yanyi Huang
Many on-chip approaches that use flow-focusing to pinch the continuous aqueous phase into droplets have become the most popular methods that provide monodisperse emulsion droplets. However, not every lab can easily adapt a microfluidic workflow into their familiar protocols. We develop an off-chip approach, spinning micro-pipette liquid emulsion (SiMPLE) generator, to produce highly stable monodisperse water-in-oil emulsions using a moving micropipette to disperse the aqueous phase in an oil-filled microcentrifuge tube...
October 24, 2016: Lab on a Chip
Md Akhlaquer Rahman, Md Mujahid, Arshad Hussain
AIMS: The purpose of the current study was to investigate the feasibility of producing solid self-emulsifying pellets using the extrusion/spheronization technique with an aim to increase the bioavailability of selected drug and also to encounter the problems associated with liquid lipid formulations. Self-emulsifying formulations are experiencing a very active development as reflected by the numerous publications and patents being granted on these systems. The patents on self-emulsifying formulation (US20036630150) and (US20006054136) helped in selecting the drug and excipients...
October 21, 2016: Recent Patents on Drug Delivery & Formulation
Zijie Wang, Guojun Liu, Shuaishuai Huang
A cotton fabric was coated with a polymer that contains both poly(dimethyl siloxane) (PDMS) and poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA). When the repeat unit number of PDMS is about three-fold that of PDMAEMA and the fabric is exposed to air, the fabric is superhydrophobic because PDMS in the coating covers the PDMAEMA chains. Upon contact with an oil-in-water emulsion, the water-soluble PDMAEMA rises to the top and the side in contact with the emulsion becomes hydrophilic. The emerged PDMAEMA chains then cause the emulsion droplets to coagulate, and the aggregated oil fills the pores on the superhydrophobic side of the fabric...
October 24, 2016: Angewandte Chemie
Pavel Trtik, Manuel Morgano, Roman Bentz, Eberhard Lehmann
The recent developments in scientific complementary metal oxide semiconductor (sCMOS) detector technology allow for imaging of relevant processes with very high temporal resolution with practically negligible readout time. However, it is neutron intensity that limits the high temporal resolution neutron imaging. In order to partially overcome the neutron intensity problem for the high temporal resolution imaging, a parabolic neutron focussing guide was utilized in the test arrangement and placed upstream the detector in such a manner that the focal point of the guide was positioned slightly behind the scintillator screen...
2016: MethodsX
Nandan S Gokhale, Alexa B R McIntyre, Michael J McFadden, Allison E Roder, Edward M Kennedy, Jorge A Gandara, Sharon E Hopcraft, Kendra M Quicke, Christine Vazquez, Jason Willer, Olga R Ilkayeva, Brittany A Law, Christopher L Holley, Mariano A Garcia-Blanco, Matthew J Evans, Mehul S Suthar, Shelton S Bradrick, Christopher E Mason, Stacy M Horner
The RNA modification N6-methyladenosine (m(6)A) post-transcriptionally regulates RNA function. The cellular machinery that controls m(6)A includes methyltransferases and demethylases that add or remove this modification, as well as m(6)A-binding YTHDF proteins that promote the translation or degradation of m(6)A-modified mRNA. We demonstrate that m(6)A modulates infection by hepatitis C virus (HCV). Depletion of m(6)A methyltransferases or an m(6)A demethylase, respectively, increases or decreases infectious HCV particle production...
October 18, 2016: Cell Host & Microbe
Stefanie Funke, Julia Matilainen, Heiko Nalenz, Karoline Bechtold-Peters, Hanns-Christian Mahler, Wolfgang Friess
A significant number of therapeutic proteins are marketed as pre-filled syringes or other drug/device combination products and have been safely used in these formats for years. Silicone oil, which is used as lubricant, can migrate into the drug product and may interact with therapeutic proteins. In this study, particles in the size range of 0.2-5 μm and ≥1 μm as determined by resonant mass measurement and micro-flow imaging/light obscuration, respectively, resulted from silicone sloughing off the container barrel after agitation...
October 20, 2016: Journal of Pharmaceutical Sciences
Xiaoming Luo, Juhang Cao, Limin He, Hongping Wang, Haipeng Yan, Yahua Qin
The coalescence process of binary droplets in oil under ultrasonic standing waves was investigated with high-speed photography. Three motion models of binary droplets in coalescence process were illustrated: (1) slight translational oscillation; (2) sinusoidal translational oscillation; (3) migration along with acoustic streaming. To reveal the droplets coalescence mechanisms, the influence of main factors (such as acoustic intensity, droplet size, viscosity and interfacial tension, etc) on the motion and coalescence of binary droplets was studied under ultrasonic standing waves...
January 2017: Ultrasonics Sonochemistry
Khushwinder Kaur, Raj Kumar, Arpita, Sumit Goel, Shivani Uppal, Alka Bhatia, S K Mehta
The main aim of the present work was to prepare TPGS stabilized D-α-Tocopherol, lemon oil, tween-80, and water nanoemulsion by low cost and highly effective sonication method. The prepared nanoemulsion showed good stability for 60days at variable temperature conditions i.e. 4, 25 and 37°C. The tolerance of the prepared nanoemulsion to salt (50mM-500mM) and pH (pH 2-pH 7.4) was also studied. The morphology and droplet size of pure and quinine loaded nanoemulsion was characterized with transmission electron microscopy...
January 2017: Ultrasonics Sonochemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"