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https://www.readbyqxmd.com/read/28034712/evaluation-of-a-digital-dispenser-for-direct-curve-dilutions-in-a-vaccine-potency-assay
#1
Christopher Roselle, Dana Whitehouse, Thy Follmer, Fran Ansbro, Silikhone Bouaraphan, Liming Guan, Sha-Ke Wang, Mary Shank-Retzlaff, Thorsten Verch
Dilutions are a common source of analytical error, both in terms of accuracy and precision, and a common source of analyst mistakes. When serial dilutions are used, errors compound, even when employing laboratory automation. Direct point dilutions instead of serial dilutions can reduce error but is often impractical as they require either large diluent volumes or very small sample volumes when performed with traditional liquid handling equipment. We evaluated preparation of dilution curves using a picoliter digital dispenser, the HP, Inc...
December 27, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28009866/centrifugal-micro-channel-array-droplet-generation-for-highly-parallel-digital-pcr
#2
Zitian Chen, Peiyu Liao, Fangli Zhang, Mengcheng Jiang, Yusen Zhu, Yanyi Huang
Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination...
December 22, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27995242/surface-acoustic-wave-enabled-pipette-on-a-chip
#3
Muhsincan Sesen, Citsabehsan Devendran, Sean Malikides, Tuncay Alan, Adrian Neild
Mono-disperse droplet formation in microfluidic devices allows the rapid production of thousands of identical droplets and has enabled a wide range of chemical and biological studies through repeat tests performed at pico-to-nanoliter volume samples. However, it is exactly this efficiency of production which has hindered the ability to carefully control the location and quantity of the distribution of various samples on a chip - the key requirement for replicating micro well plate based high throughput screening in vastly reduced volumetric scales...
December 20, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27993964/plasma-circulating-tumor-dna-in-pancreatic-cancer-patients-is-a-prognostic-marker
#4
Daniel Pietrasz, Nicolas Pécuchet, Fanny Garlan, Audrey Didelot, Olivier Dubreuil, Solène Doat, Francoise Imbert-Bismut, Mehdi Karoui, Jean-Christophe Vaillant, Valérie Taly, Pierre Laurent-Puig, Jean-Baptiste Bachet
PURPOSE: Despite recent therapeutic advances, prognosis of patients with pancreatic adenocarcinoma remains poor. Analyses from tumor tissues present limitations; identification of informative marker from blood might be a promising alternative. The aim of this study was to assess the feasibility and the prognostic value of circulating tumor DNA (ctDNA) in pancreatic adenocarcinoma. EXPERIMENTAL DESIGN: From 2011 to 2015, blood samples were prospectively collected from all consecutive patients with pancreatic adenocarcinoma treated in our center...
January 1, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27933984/statistical-thermodynamic-model-for-surface-tension-of-organic-and-inorganic-aqueous-mixtures
#5
Hallie C Boyer, Bryan R Bzdek, Jonathan P Reid, Cari S Dutcher
The surface composition and tensions of aqueous aerosols govern a set of processes that largely determine the fate of particles in the atmosphere. Predictive modeling of surface tension can provide significant contributions to studies of atmospheric aerosol effects on climate and human health. A previously derived surface tension model for single solute aqueous solutions used adsorption isotherms and statistical mechanics to enable surface tension predictions across the entire concentration range as a function of solute activity...
December 30, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27933691/single-fusion-events-at-polarized-liquid-liquid-interfaces
#6
Eduardo Laborda, Angela Molina, Vanesa Fernández Espín, Francisco Martínez-Ortiz, José García de la Torre, Richard G Compton
A new electrochemical framework for tracking individual soft particles in solution and monitoring their fusion with polarized liquid-liquid interfaces is reported. The physicochemical principle lies in the interfacial transfer of an ionic probe confined in the particles dispersed in solution and that is released upon their collision and fusion with the fluid interface. As a proof-of-concept, spike-like transients of a stochastic nature are reported in the current-time response of 1,2-dichloroethane(DCE)|water(W) submilli-interfaces after injection of DCE-in-W emulsions...
December 9, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27860309/a-platform-for-electric-field-aided-and-wire-guided-droplet-manipulation
#7
Yanzhen Zhang, Gunther Wittstock
Small droplets can be manipulated based on controlling the adhesion work to a hydrophobic wire. The wire can be used to pick up, transport, and lay down droplets with volumes between picoliters to microliters avoiding the sliding of droplets over chip surfaces. Handling of droplets on surfaces with large steps such as engraved wells or channels is possible.
November 14, 2016: Small
https://www.readbyqxmd.com/read/27812580/monitoring-few-molecular-binding-events-in-scalable-confined-aqueous-compartments-by-raster-image-correlation-spectroscopy-cadrics
#8
G Arrabito, F Cavaleri, V Montalbano, V Vetri, M Leone, B Pignataro
The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing is employed to develop up to hundreds of picoliter aqueous droplet arrays stabilized by oil-confinement with mild surfactants (Tween-20). The aqueous environments constitute specialized compartments in which biomolecules may exploit their function and a wide range of molecular interactions can be quantitatively investigated...
November 4, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27783484/single-cell-total-rna-sequencing-through-isothermal-amplification-in-picoliter-droplet-emulsion
#9
Yusi Fu, He Chen, Lu Liu, Yanyi Huang
Prevalent single cell RNA amplification and sequencing chemistries mainly focus on polyadenylated RNAs in eukaryotic cells by using oligo(dT) primers for reverse transcription. We develop a new RNA amplification method, "easier-seq", to reverse transcribe and amplify the total RNAs, both with and without polyadenylate tails, from a single cell for transcriptome sequencing with high efficiency, reproducibility, and accuracy. By distributing the reverse transcribed cDNA molecules into 1.5 × 10(5) aqueous droplets in oil, the cDNAs are isothermally amplified using random primers in each of these 65-pL reactors separately...
November 15, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27782402/3d-printing-of-photoresponsive-biomaterials-for-control-of-bacterial-microenvironments
#10
Jodi L Connell, Eric T Ritschdorff, Jason B Shear
Advances in microscopic three-dimensional (µ3D) printing provide a means to microfabricate an almost limitless range of arbitrary geometries, offering new opportunities to rapidly prototype complex architectures for microfluidic and cellular applications. Such 3D lithographic capabilities present the tantalizing prospect for engineering micromechanical components - for example, pumps and valves - for cellular environments comprised of smart materials whose size, shape, permeability, stiffness, and other attributes might be modified in real time to precisely manipulate ultra-low-volume samples...
October 26, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27775138/spinning-micropipette-liquid-emulsion-generator-for-single-cell-whole-genome-amplification
#11
Zitian Chen, Yusi Fu, Fangli Zhang, Lu Liu, Naiqing Zhang, Dong Zhou, Junrui Yang, Yuhong Pang, Yanyi Huang
Many on-chip approaches that use flow-focusing to pinch the continuous aqueous phase into droplets have become the most popular methods that provide monodisperse emulsion droplets. However, not every lab can easily adapt a microfluidic workflow into their familiar protocols. We develop an off-chip approach, spinning micro-pipette liquid emulsion (SiMPLE) generator, to produce highly stable monodisperse water-in-oil emulsions using a moving micropipette to disperse the aqueous phase in an oil-filled microcentrifuge tube...
October 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27714122/optofluidic-sensing-from-inkjet-printed-droplets-the-enormous-enhancement-by-evaporation-induced-spontaneous-flow-on-photonic-crystal-biosilica
#12
Xianming Kong, Yuting Xi, Paul LeDuff, Erwen Li, Ye Liu, Li-Jing Cheng, Gregory L Rorrer, Hua Tan, Alan X Wang
Novel transducers for detecting an ultra-small volume of an analyte solution play pivotal roles in many applications such as chemical analysis, environmental protection and biomedical diagnosis. Recent advances in optofluidics offer tremendous opportunities for analyzing miniature amounts of samples with high detection sensitivity. In this work, we demonstrate enormous enhancement factors (10(6)-10(7)) of the detection limit for optofluidic analysis from inkjet-printed droplets by evaporation-induced spontaneous flow on photonic crystal biosilica when compared with conventional surface-enhanced Raman scattering (SERS) sensing using the pipette dispensing technology...
October 6, 2016: Nanoscale
https://www.readbyqxmd.com/read/27677315/label-free-analysis-and-sorting-of-microalgae-and-cyanobacteria-in-microdroplets-by-intrinsic-chlorophyll-fluorescence-for-the-identification-of-fast-growing-strains
#13
Roshni J Best, Jan J Lyczakowski, Sara Abalde-Cela, Ziyi Yu, Chris Abell, Alison G Smith
Microalgae and cyanobacteria are promising organisms for sustainable biofuel production, but several challenges remain to make this economically viable, including identification of optimized strains with high biomass productivity. Here we report on a novel methodology for the label-free screening and sorting of cyanobacteria and microalgae in a microdroplet platform. We show for the first time that chlorophyll fluorescence can be used to measure differences in biomass between populations of picoliter microdroplets containing different species of cyanobacteria, Synechocystis PCC 6803 and Synechococcus PCC 7002, which exhibit different growth dynamics in bulk culture...
October 21, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27596612/label-free-isolation-and-deposition-of-single-bacterial-cells-from-heterogeneous-samples-for-clonal-culturing
#14
J Riba, T Gleichmann, S Zimmermann, R Zengerle, P Koltay
The isolation and analysis of single prokaryotic cells down to 1 μm and less in size poses a special challenge and requires micro-engineered devices to handle volumes in the picoliter to nanoliter range. Here, an advanced Single-Cell Printer (SCP) was applied for automated and label-free isolation and deposition of bacterial cells encapsulated in 35 pl droplets by inkjet-like printing. To achieve this, dispenser chips to generate micro droplets have been fabricated with nozzles 20 μm in size. Further, the magnification of the optical system used for cell detection was increased...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27495973/bacterial-ice-nucleation-in-monodisperse-d2o-and-h2o-in-oil-emulsions
#15
Lindong Weng, Shannon N Tessier, Kyle Smith, Jon F Edd, Shannon L Stott, Mehmet Toner
Ice nucleation is of fundamental significance in many areas, including atmospheric science, food technology, and cryobiology. In this study, we investigated the ice-nucleation characteristics of picoliter-sized drops consisting of different D2O and H2O mixtures with and without the ice-nucleating bacteria Pseudomonas syringae. We also studied the effects of commonly used cryoprotectants such as ethylene glycol, propylene glycol, and trehalose on the nucleation characteristics of D2O and H2O mixtures. The results show that the median freezing temperature of the suspension containing 1 mg/mL of a lyophilized preparation of P...
September 13, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27427710/imitating-musical-scores-on-a-chip-through-droplet-generation
#16
Xiaoyun Shawn Yang, Soojeong Shin, Woon Seob Lee, Jong Wook Hong
We have demonstrated fine control of droplet volumes on a chip using pneumatically actuated micro valve systems resulting in confined nanoliter or picoliter reagent generation. Using the micro valves, we are able to align multi-inlet droplet generating chips for systematic generation of combinatorial mixtures of chemical constituents. By following the change of chemical conditions such as pH we converted musical note information into sequential droplets representing musical scores of 'Twinkle little star.' It would just one of the interesting application of micro-droplet generation systems based on accurate droplet generation, mixing, and incubation...
June 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27419874/tunable-single-cell-extraction-for-molecular-analyses
#17
Orane Guillaume-Gentil, Rashel V Grindberg, Romain Kooger, Livie Dorwling-Carter, Vincent Martinez, Dario Ossola, Martin Pilhofer, Tomaso Zambelli, Julia A Vorholt
Because of cellular heterogeneity, the analysis of endogenous molecules from single cells is of significant interest and has major implications. While micromanipulation or cell sorting followed by cell lysis is already used for subsequent molecular examinations, approaches to directly extract the content of living cells remain a challenging but promising alternative to achieving non-destructive sampling and cell-context preservation. Here, we demonstrate the quantitative extraction from single cells with spatiotemporal control using fluidic force microscopy...
July 14, 2016: Cell
https://www.readbyqxmd.com/read/27416070/multiplex-detection-of-rare-mutations-by-picoliter-droplet-based-digital-pcr-sensitivity-and-specificity-considerations
#18
Eleonora Zonta, Fanny Garlan, Nicolas Pécuchet, Karla Perez-Toralla, Ouriel Caen, Coren Milbury, Audrey Didelot, Elizabeth Fabre, Hélène Blons, Pierre Laurent-Puig, Valérie Taly
In cancer research, the accuracy of the technology used for biomarkers detection is remarkably important. In this context, digital PCR represents a highly sensitive and reproducible method that could serve as an appropriate tool for tumor mutational status analysis. In particular, droplet-based digital PCR approaches have been developed for detection of tumor-specific mutated alleles within plasmatic circulating DNA. Such an approach calls for the development and validation of a very significant quantity of assays, which can be extremely costly and time consuming...
2016: PloS One
https://www.readbyqxmd.com/read/27377052/a-compact-short-capillary-based-high-speed-capillary-electrophoresis-bioanalyzer
#19
Xiao-Xia Fang, Pan Fang, Jian-Zhang Pan, Qun Fang
Here, a compact high-speed CE bioanalyzer based on a short capillary has been developed. Multiple modules of picoliter scale sample injection, high-speed CE separation, sample changing, LIF detection, as well as a custom designed tablet computer for data processing, instrument controlling, and result displaying were integrated in the bioanalyzer with a total size of 23 × 17 × 19 cm (length × width × height). The high-speed CE bioanalyzer is capable of performing automated sample injection and separation for multiple samples and has been successfully applied in fast separations of amino acids, chiral amino acids, proteins and DNA fragments...
September 2016: Electrophoresis
https://www.readbyqxmd.com/read/27285343/oxygen-purged-microfluidic-device-to-enhance-cell-viability-in-photopolymerized-peg-hydrogel-microparticles
#20
Bingzhao Xia, Kaspars Krutkramelis, John Oakey
Encapsulating cells within biocompatible materials is a widely used strategy for cell delivery and tissue engineering. While cells are commonly suspended within bulk hydrogel-forming solutions during gelation, substantial interest in the microfluidic fabrication of miniaturized cell encapsulation vehicles has more recently emerged. Here, we utilize multiphase microfluidics to encapsulate cells within photopolymerized picoliter-volume water-in-oil droplets at high production rates. The photoinitiated polymerization of polyethylene glycol diacrylate (PEGDA) is used to continuously produce solid particles from aqueous liquid drops containing cells and hydrogel forming solution...
July 11, 2016: Biomacromolecules
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