Simon Andreas Mayer, Benjamin Thomas, Miriam Heuer, Jan Brune, Volker Eras, Kilian Schuster, Leonard Knoedler, Rebecca Luisa Schäfer, Wilko Thiele, Jonathan Sleeman, Arno Dimmler, Patrick Heimel, Ulrich Kneser, Amir K Bigdeli, Florian Falkner
The arteriovenous loop (AVL) model allows the in vivo engineering of axially vascularized flaps, the so called AVL flaps. Although AVL flaps can be transplanted microsurgically to cover tissue defects, they lack an epithelial layer on the surface. Therefore, the objective of this study was to engineer axially vascularized AVL flaps with an accompanying epithelial layer for local defect reconstruction. In this study AVLs were established in 20 male Lewis rats. Minimally invasive injection of keratinocytes onto the surface of the AVL flaps was performed on postoperative day (POD) 21...
April 16, 2024: Tissue Engineering. Part A