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https://www.readbyqxmd.com/read/29202298/impact-of-ph-and-application-time-of-meta-phosphoric-acid-on-resin-enamel-and-resin-dentin-bonding
#1
A F M Cardenas, F S F Siqueira, M C Bandeca, S O Costa, M V S Lemos, V P Feitora, A Reis, A D Loguercio, J C Gomes
PURPOSE: To evaluate the immediate microshear resin-enamel bond strength (μSBS) and the immediate and 6-month microtensile bond strength (μTBS) and nanoleakage (NL) of the adhesive interface performed by different pHs of 40% meta-phosphoric acid (MPA) were compared with conventional 37% ortho-phosphoric acid (OPA) under different application times. Additionally, the enamel etching patterns were evaluated and the chemical/morphological changes induced by these differents groups were evaluated...
November 22, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29186932/ionic-substitutions-in-non-apatitic-calcium-phosphates
#2
REVIEW
Aleksandra Laskus, Joanna Kolmas
Calcium phosphate materials (CaPs) are similar to inorganic part of human mineralized tissues (i.e., bone, enamel, and dentin). Owing to their high biocompatibility, CaPs, mainly hydroxyapatite (HA), have been investigated for their use in various medical applications. One of the most widely used ways to improve the biological and physicochemical properties of HA is ionic substitution with trace ions. Recent developments in bioceramics have already demonstrated that introducing foreign ions is also possible in other CaPs, such as tricalcium phosphates (amorphous as well as α and β crystalline forms) and brushite...
November 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29161895/editorial-comment-on-end-2017-0436-or-r1
#3
Michael Grasso
A central component of percutaneous nephrostolithotomy is an endoscopic lithotrite that safely and efficiently clears stone of all compositions from the intrarenal collecting system. The current multicenter trial studied 3 lithotrite combinations employing both mechanical ballistic and ultrasonic energy to fragment and evacuate with suction stone debris. What is clear from this work is that, with regard to the timely clearance of the stone burden and peri-operative complications, there is minimal difference between the devices studied, nothing special and nothing new...
November 22, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/29105970/effects-of-platelet-rich-plasma-on-biological-activity-and-bone-regeneration-of-brushite-based-calcium-phosphate-cement
#4
Md Lemon Hasan, Mirana Taz, Byong-Taek Lee
In this study, we focused on identifying the effects of platelet-rich plasma (PRP) on bioactivity and bone ingrowths incorporated into brushite cement. We introduced PRP as a series of substitutions with an aqueous citrate-ion solution, and the optimized cement with PRP showed no disintegration of the paste consistency. Incorporating PRP showed that the setting time decreased with the increasing of PRP ratios, although the compressive strength was not significantly changed. We evaluated in vitro degradation and bioactivity with the simulated body fluid test, and the result showed that adding PRP accelerated the carbonated apatite nucleation and markedly improved the surface reactivity of the cement...
November 6, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29102397/short-time-pre-washing-of-brushite-forming-calcium-phosphate-cement-improves-its-in-vitro-cytocompatibility
#5
Elke Kunisch, Stefan Maenz, Marie Knoblich, Frank Ploeger, Klaus D Jandt, Joerg Bossert, Raimund W Kinne, Saifeddin Alsalameh
A pre-washing protocol was developed for resorbable, brushite-forming calcium phosphate cements (CPCs) to avoid harmful in vitro effects on cells. CPC discs (JectOS+, Kasios; self-developed CPC) were pre-washed with repeated changes of phosphate-buffered saline (PBS; 24h total). Unwashed or PBS-pre-washed discs were incubated in culture medium (5% fetal calf serum; up to 10days) and then tested for their influence on pH/calcium/phosphate levels in H2O extracts. Effects on pH/calcium/phosphate levels in culture supernatants, and morphology, adherence, number, and viability of ATDC5 cells and adipose-tissue derived stem cells were analyzed in co-culture...
October 14, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29096930/long-term-radiographic-appearance-of-calcium-phosphate-synthetic-bone-grafts-after-surgical-treatment-of-tibial-plateau-fractures
#6
Alexander Hanke, Martin Bäumlein, Siegmund Lang, Boyko Gueorguiev, Michael Nerlich, Thomas Perren, Paavo Rillmann, Christian Ryf, Theodore Miclau, Markus Loibl
OBJECTIVES: Synthetic bone grafts (SBGs) are widely used to fill bone defects after fracture reduction. This study assessed the long-term resorption of two different calcium phosphate products (A=ChronOS™ inject and B=Norian(®) SRS(®); both DePuy Synthes, Oberdorf, Switzerland) used in the surgical treatment of tibial plateau fractures. DESIGN: Long-term clinical and radiologic follow-up of 52 patients after surgical treatment of intraarticular tibial plateau fractures augmented with SBGs...
October 19, 2017: Injury
https://www.readbyqxmd.com/read/29079514/dual-modulation-of-bone-formation-and-resorption-with-zoledronic-acid-loaded-biodegradable-magnesium-alloy-implants-improves-osteoporotic-fracture-healing-an-in-vitro-and-in-vivo-study
#7
Guoyuan Li, Lei Zhang, Lei Wang, Guangyin Yuan, Kerong Dai, Jia Pei, Yongqiang Hao
Osteoporotic fracture (OPF) remains a major clinical challenge for skeletal regeneration. Impaired osteogenesis and excessive remodeling result in prolonged and poor quality of fracture healing. To augment bone formation and inhibit excessive resorption simultaneously, we constructed a biodegradable magnesium-based implant integrated with the anti-catabolic drug zoledronic acid (ZA); this implant exhibits controllable, sustained release of magnesium degradation products and ZA in vitro. The extracts greatly stimulate the osteogenic differentiation of rat-bone marrow-derived mesenchymal stem cells (rBMSCs), while osteoclastogenesis is inhibited by ZA...
October 24, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29031993/the-gdf5-mutant-bb-1-enhances-the-bone-formation-induced-by-an-injectable-plga-fiber-reinforced-brushite-forming-cement-in-a-sheep-defect-model-of-lumbar-osteopenia
#8
Francesca Gunnella, Elke Kunisch, Stefan Maenz, Victoria Horbert, Long Xin, Joerg Mika, Juliane Borowski, Sabine Bischoff, Harald Schubert, Andre Sachse, Bernhard Illerhaus, Jens Günster, Jörg Bossert, Klaus D Jandt, Frank Plöger, Raimund W Kinne, Olaf Brinkmann, Matthias Bungartz
BACKGROUND CONTEXT: Targeted delivery of osteoinductive bone morphogenetic proteins (BMPs; e.g., GDF5) in bioresorbable calcium phosphate cement (CPC), potentially suitable for vertebroplasty/kyphoplasty of osteoporotic vertebral fractures, may be required to counteract augmented local bone catabolism and support complete bone regeneration. The biologically optimized GDF5 mutant BB-1 may represent an attractive drug candidate for this purpose. PURPOSE: The aim of the current study was to test an injectable, poly (l-lactide-co-glycolide) acid (PLGA)-fiber reinforced, brushite-forming cement (CPC) containing low-dose BB-1 in a sheep lumbar osteopenia model...
October 12, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28962775/new-in-situ-synthetized-hydrogel-composite-based-on-alginate-and-brushite-as-a-potential-ph-sensitive-drug-delivery-system
#9
Seyed Mohammad Hossein Dabiri, Alberto Lagazzo, Fabrizio Barberis, Amirreza Shayganpour, Elisabetta Finocchio, Laura Pastorino
A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels. The Bru crystals were synthetized and dispersed during the crosslinking process of Alg matrix. The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling...
December 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28948757/in-situ-synchrotron-x-ray-diffraction-analysis-of-the-setting-process-of-brushite-cement-reaction-and-crystal-growth
#10
Jun Luo, Francisco Javier Martinez-Casado, Olivier Balmes, Jiaojiao Yang, Cecilia Persson, Håkan Engqvist, Wei Xia
Brushite cements are fast self-setting materials that can be used as bone substitute materials. Although tracing their fast setting process is a challenge, it is important for the understanding of the same, which in turn is important for the material's further development and use in the clinics. In this study, the setting rate, phase formation, and crystal growth of brushite cements were quantitatively studied by in situ synchrotron powder X-ray diffraction (SXRD) on a time scale of seconds. The influence of reactant ratios and a retardant (citric acid) on the setting reaction were analyzed...
October 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28945133/dual-energy-computed-tomography-of-canine-uroliths
#11
Stephanie G Nykamp
OBJECTIVE To determine whether dual-energy CT (DECT) could accurately differentiate the composition of common canine uroliths in a phantom model. SAMPLE 30 canine uroliths with pure compositions. PROCEDURES Each urolith was composed of ≥ 70% struvite (n = 10), urate (8), cystine (5), calcium oxalate (4), or brushite (3) as determined by standard laboratory methods performed at the Canadian Veterinary Urolith Centre. Uroliths were suspended in an agar phantom, and DECT was performed at low (80 kV) and high (140 kV) energies...
October 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28918134/crystallization-and-demineralization-phenomena-in-washed-rind-cheese
#12
Gil F Tansman, Paul S Kindstedt, John M Hughes
This report documents an observational study of a high-moisture washed-rind cheese. Three batches of cheese were sampled on a weekly basis for 6 wk and again at wk 10. Center, under-rind, rind, and smear samples were tested for pH, moisture, and selected mineral elements. Powder x-ray diffractometry and petrographic microscopy were applied to identify and image the crystal phases. The pH of the rind increased by over 2 pH units by wk 10. The pH of the under-rind increased but remained below the rind pH, whereas the center pH decreased for most of aging and only began to rise after wk 5...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28916883/different-post-processing-conditions-for-3d-bioprinted-%C3%AE-tricalcium-phosphate-scaffolds
#13
Liciane Sabadin Bertol, Rodrigo Schabbach, Luis Alberto Loureiro Dos Santos
The development of 3D printing hardware, software and materials has enabled the production of bone substitute scaffolds for tissue engineering. Calcium phosphates cements, such as those based on α-tricalcium phosphate (α-TCP), have recognized properties of osteoinductivity, osteoconductivity and resorbability and can be used to 3D print scaffolds to support and induce tissue formation and be replaced by natural bone. At present, however, the mechanical properties found for 3D printed bone scaffolds are only satisfactory for non-load bearing applications...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28882031/a-multiregional-italian-cohort-of-24-hour-urine-metabolic-evaluation-in-renal-stone-formers
#14
Francesco Esperto, Martino Marangella, Alberto Trinchieri, Michele Petrarulo, Roberto Miano
BACKGROUND: Nephrolithiasis is a common condition with several studies documenting an increased prevalence over the past four decades. EAU and AUA guidelines recommend 24-hour urine metabolic evaluation in high-risk stone formers. Aim of this study is to retrospectively evaluate the first three years of experience with Lithotest® (Biohealth Italia Srl, Turin, Italy) through the analysis of demographic, clinical and biochemical data collected from a large cohort of patients with kidney stones...
September 7, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/28866230/strontium-doped-injectable-bone-cement-for-potential-drug-delivery-applications
#15
Ali Taha, Muhammad Akram, Zaidoon Jawad, Ammar Z Alshemary, Rafaqat Hussain
Microwave assisted wet precipitation method was used to synthesize calcium deficient strontium doped β-tricalcium phosphate (Sr-βTCP) with a chemical formula of Ca2.96-xSrx(PO4)2. Sr-βTCP was reacted with monocalcium phosphate monohydrate [Ca(H2PO4)2.H2O, MCPM] in presence of water to furnish corresponding Sr containing brushite cement (Sr-Brc). The samples were characterized by using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM)...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28841980/electrochemical-enzyme-biosensors-based-on-calcium-phosphate-materials-for-tyramine-detection-in-food-samples
#16
Marta Sánchez-Paniagua López, Esther Redondo-Gómez, Beatriz López-Ruiz
Electrochemical tyrosinase biosensors for tyramine determination were developed by the immobilization of the enzyme in calcium phosphate materials (CaPs) followed by cross-linking with glutaraldehyde. Tyramine was detected by the electrochemical reduction at -0.1V of the o- enzymatically-formed dopaquinone. Three different CaPs were explored as immobilization systems, monetite, brushite and brushite cement. Biosensors based on brushite matrices provide better analytical properties than the monetite one. Compared to brushite, a 10-fold increase of sensitivity was obtained with the brushite cement-based biosensor, which highlights the effect of brushite crystal formation in the presence of the enzyme in the biosensor performance...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28822796/papillary-ductal-plugging-is-a-mechanism-for-early-stone-retention-in-brushite-stone-disease
#17
James C Williams, Michael S Borofsky, Sharon B Bledsoe, Andrew P Evan, Fredric L Coe, Elaine M Worcester, James E Lingeman
PURPOSE: Mechanisms of early stone retention in the kidney are under studied and poorly understood. To date attachment via Randall plaque is the only widely accepted theory in this regard, which is best described in idiopathic calcium oxalate stone formers. Brushite stone formers are known to have distinct papillary morphology relative to calcium oxalate stone formers. As such we sought to determine whether stone attachment mechanisms in such patients may be similarly unique. MATERIALS AND METHODS: Patients undergoing percutaneous and or ureteroscopic procedures for stone removal consented to endoscopic renal papillary examination and individual stone collection...
August 16, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28808430/amelogenin-affects-brushite-crystal-morphology-and-promotes-its-phase-transformation-to-monetite
#18
Dongni Ren, Qichao Ruan, Jinhui Tao, Jonathan Lo, Steven Nutt, Janet Moradian-Oldak
Amelogenin protein is involved in organized apatite crystallization during enamel formation. Brushite (CaHPO4·2H2O), one of the precursors of hydroxyapatite mineralization in vitro, has been used for fabrication of biomaterials for hard tissue repair. In order to explore its potential application in biomimetic material synthesis, we studied the influence of the enamel protein amelogenin on brushite morphology and phase transformation to monetite. Our results show that amelogenin can adsorb onto the surface of brushite, leading to the formation of layered morphology on the (010) face...
September 7, 2016: Crystal Growth & Design
https://www.readbyqxmd.com/read/28794471/quantifying-the-efficiency-of-hydroxyapatite-mineralising-peptides
#19
Robyn Plowright, David J Belton, David L Kaplan, Carole C Perry
We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0-100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28763481/osteoclastic-differentiation-and-resorption-is-modulated-by-bioactive-metal-ions-co2-cu2-and-cr3-incorporated-into-calcium-phosphate-bone-cements
#20
Anne Bernhardt, Martha Schamel, Uwe Gbureck, Michael Gelinsky
Biologically active metal ions in low doses have the potential to accelerate bone defect healing. For successful remodelling the interaction of bone graft materials with both bone-forming osteoblasts and bone resorbing osteoclasts is crucial. In the present study brushite forming calcium phosphate cements (CPC) were doped with Co2+, Cu2+ and Cr3+ and the influence of these materials on osteoclast differentiation and activity was examined. Human osteoclasts were differentiated from human peripheral blood mononuclear cells (PBMC) both on the surface and in indirect contact to the materials on dentin discs...
2017: PloS One
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