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https://www.readbyqxmd.com/read/28723420/plant-and-yeast-cornichon-possess-a-conserved-acidic-motif-required-for-correct-targeting-of-plasma-membrane-cargos
#1
Paul Rosas-Santiago, Daniel Lagunas-Gomez, Carolina Yáñez-Domínguez, Rosario Vera-Estrella, Olga Zimmermannova, Hana Sychrová, Omar Pantoja
The export of membrane proteins along the secretory pathway is initiated at the endoplasmic reticulum after proteins are folded and packaged inside this organelle by their recruiting into the coat complex COPII vesicles. It is proposed that cargo receptors are required for the correct transport of proteins to its target membrane, however, little is known about ER export signals for cargo receptors. Erv14/Cornichon belong to a well conserved protein family in Eukaryotes, and have been proposed to function as cargo receptors for many transmembrane proteins...
July 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28713858/life-stage-specific-cargo-receptors-facilitate-glycosylphosphatidylinositol-anchored-surface-coat-protein-transport-in-trypanosoma-brucei
#2
Emilia K Kruzel, George P Zimmett, James D Bangs
The critical virulence factor of bloodstream-form Trypanosoma brucei is the glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG). Endoplasmic reticulum (ER) exit of VSG is GPI dependent and relies on a discrete subset of COPII machinery (TbSec23.2/TbSec24.1). In other systems, p24 transmembrane adaptor proteins selectively recruit GPI-anchored cargo into nascent COPII vesicles. Trypanosomes have eight putative p24s (TbERP1 to TbERP8) that are constitutively expressed at the mRNA level...
July 2017: MSphere
https://www.readbyqxmd.com/read/28706295/dgk%C3%AE-triggers-endoplasmic-reticulum-release-of-ift88-containing-vesicles-destined-for-the-assembly-of-primary-cilia
#3
Jie Ding, Lei Shao, Yixing Yao, Xin Tong, Huaize Liu, Shen Yue, Lu Xie, Steven Y Cheng
The morphogenic factor Sonic hedgehog (Shh) signals through the primary cilium, which relies on intraflagellar transport to maintain its structural integrity and function. However, the process by which protein and lipid cargos are delivered to the primary cilium from their sites of synthesis still remains poorly characterized. Here, we report that diacylglycerol kinase δ (DGKδ), a residential lipid kinase in the endoplasmic reticulum, triggers the release of IFT88-containing vesicles from the ER exit sites (ERES), thereby setting forth their movement to the primary cilium...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28700938/golgi-outpost-synthesis-impaired-by-toxic-polyglutamine-proteins-contributes-to-dendritic-pathology-in-neurons
#4
Chang Geon Chung, Min Jee Kwon, Keun Hye Jeon, Do Young Hyeon, Myeong Hoon Han, Jeong Hyang Park, In Jun Cha, Jae Ho Cho, Kunhyung Kim, Sangchul Rho, Gyu Ree Kim, Hyobin Jeong, Jae Won Lee, TaeSoo Kim, Keetae Kim, Kwang Pyo Kim, Michael D Ehlers, Daehee Hwang, Sung Bae Lee
Dendrite aberration is a common feature of neurodegenerative diseases caused by protein toxicity, but the underlying mechanisms remain largely elusive. Here, we show that nuclear polyglutamine (polyQ) toxicity resulted in defective terminal dendrite elongation accompanied by a loss of Golgi outposts (GOPs) and a decreased supply of plasma membrane (PM) in Drosophila class IV dendritic arborization (da) (C4 da) neurons. mRNA sequencing revealed that genes downregulated by polyQ proteins included many secretory pathway-related genes, including COPII genes regulating GOP synthesis...
July 11, 2017: Cell Reports
https://www.readbyqxmd.com/read/28697897/measuring-protein-binding-to-lipid-vesicles-by-fluorescence-cross-correlation-spectroscopy
#5
Daniela Krüger, Jan Ebenhan, Stefan Werner, Kirsten Bacia
Fluorescence correlation spectroscopy has been previously used to investigate peptide and protein binding to lipid membranes, as it allows for very low amounts of sample, short measurement times and equilibrium binding conditions. Labeling only one of the binding partners, however, comes with certain drawbacks, as it relies on identifying binding events by a change in diffusion coefficient. Since peptide and protein aggregation can obscure specific binding, and since non-stoichiometric binding necessitates the explicit choice of a statistical distribution for the number of bound ligands, we additionally label the liposomes and perform dual-color fluorescence cross-correlation spectroscopy (dcFCCS)...
July 8, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28696565/the-er-is-the-sorting-core-facility-in-the-golgi-lacking-protozoan-giardia-lamblia
#6
Nahuel Zamponi, Emiliano Zamponi, Gonzalo F Mayol, Adriana Lanfredi-Rangel, Staffan G Svärd, María C Touz
Our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. Here, we show that, in the absence of a Golgi compartment in Giardia, proteins destined for secretion are directly sorted and packaged at specialized ER regions enriched in COPII coatomer complexes and ceramide. We also demonstrated that ER-resident proteins are retained at the ER by the action of a KDEL receptor, which, in contrast to other eukaryotic KDEL receptors, showed no interorganellar dynamic but instead acts specifically at the limit of the ER membrane...
July 11, 2017: Traffic
https://www.readbyqxmd.com/read/28688928/transport-from-the-endoplasmic-reticulum-to-the-golgi-in-plants-where-are-we-now
#7
REVIEW
Federica Brandizzi
The biogenesis of about one third of the cellular proteome is initiated in the endoplasmic reticulum (ER), which exports proteins to the Golgi apparatus for sorting to their final destination. Notwithstanding the close proximity of the ER with other secretory membranes (e.g., endosomes, plasma membrane), the ER is also important for the homeostasis of non-secretory organelles such as mitochondria, peroxisomes, and chloroplasts. While how the plant ER interacts with most of the non-secretory membranes is largely unknown, the knowledge on the mechanisms for ER-to-Golgi transport is relatively more advanced...
July 5, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28679754/sec24c-dependent-transport-of-claudin-1-regulates-hepatitis-c-virus-entry
#8
Peiqi Yin, Ye Li, Leiliang Zhang
Claudin-1 is an HCV co-receptor required for viral entry. Although extensive studies have focused on claudin-1 as an anti-HCV target, little is known about how the level of claudin-1 in cell surface is regulated by host vesicular transport. Here, we identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the COPII vesicular transport system. By interacting with Sec24C through its C-terminal YV, claudin-1 is transported from the ER and is eventually targeted into cell surface. Blocking COPII transport inhibits HCV entry by reducing the level of claudin-1 in cell surface...
July 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28648601/inhibition-of-pld1-activity-causes-er-stress-via-regulation-of-copii-vesicle-formation
#9
Hiroshi Nakagawa, Kazuki Hazama, Katsumasa Ishida, Masayuki Komori, Kazuhiko Nishimura, Saburou Matsuo
Phospholipase D (PLD) plays a crucial role in the regulation of some cellular processes, including autophagy and apoptosis. Accumulation of protein in the endoplasmic reticulum (ER) lumen causes ER stress. Although ER stress is a principal cause of apoptosis and autophagy, the relationship between PLD activity and ER stress remains unclear. Protein transport from the ER to the Golgi apparatus is conducted by coat complex II (COPII) transport vesicles. Here, we demonstrated that inhibition of PLD1 activity or PLD1 knockdown suppressed COPII vesicle transport in normal rat kidney (NRK) cells...
June 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28626835/disrupted-er-to-golgi-trafficking-underlies-anti-hiv-drugs-and-alcohol-induced-cellular-stress-and-hepatic-injury
#10
Hui Han, Yuxin He, Jay Hu, Rhema Lau, Harrison Lee, Cheng Ji
Endoplasmic reticulum (ER) stress and unfolded protein response (UPR) are involved in anti-human immunodeficiency virus (HIV) drugs and alcohol-induced liver disease in a significant number of patients infected with HIV. However, the precise mechanism by which the drugs and alcohol cause ER stress remains elusive. We found that ritonavir-boosted lopinavir (RL) activated two canonical UPR branches without activation of the third canonical activating transcription factor 6 (ATF6) branch in either HepG2 cells or primary mouse hepatocytes...
April 2017: Hepatol Commun
https://www.readbyqxmd.com/read/28594326/er-retention-is-imposed-by-copii-protein-sorting-and-attenuated-by-4-phenylbutyrate
#11
Wenfu Ma, Elena Goldberg, Jonathan Goldberg
Native cargo proteins exit the endoplasmic reticulum (ER) in COPII-coated vesicles, whereas resident and misfolded proteins are substantially excluded from vesicles by a retention mechanism that remains unresolved. We probed the ER retention process using the proteostasis regulator 4-phenylbutyrate (4-PBA), which we show targets COPII protein to reduce the stringency of retention. 4-PBA competes with p24 proteins to bind COPII. When p24 protein uptake is blocked, COPII vesicles package resident proteins and an ER-trapped mutant LDL receptor...
June 8, 2017: ELife
https://www.readbyqxmd.com/read/28576830/gtpase-sar1-regulates-the-trafficking-and-secretion-of-the-virulence-factor-gp63-in-leishmania
#12
Smriti Parashar, Amitabha Mukhopadhyay
Metalloprotease gp63 (Ldgp63) is a critical virulence factor secreted by Leishmania. However, how newly synthesized Ldgp63 exits the endoplasmic reticulum (ER) and is secreted by this parasite is unknown. Here, we cloned, expressed, and characterized the GTPase LdSar1, and other COPII components like LdSec23, LdSec24, LdSec13, and LdSec31 from Leishmania to understand their role in ER exit of Ldgp63. Using dominant-positive (LdSar1:H74L) and dominant-negative (LdSar1:T34N) mutants of LdSar1, we found that GTP-bound LdSar1 specifically binds to LdSec23, which binds, in turn, with LdSec241-702 to form a prebudding complex...
June 2, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28559361/adaptor-protein-3-dependent-vacuolar-trafficking-involves-a-subpopulation-of-copii-and-hops-tethering-proteins
#13
Qiang-Nan Feng, Shi-Jian Song, Shi-Xia Yu, Jia-Gang Wang, Sha Li, Yan Zhang
Plant vacuoles are versatile organelles critical for plant growth and responses to environment. Vacuolar proteins are transported from the endoplasmic reticulum via multiple routes in plants. Two classic routes bear great similarity to other phyla with major regulators known, such as COPII and Rab5 GTPases. By contrast, vacuolar trafficking mediated by adaptor protein-3 (AP-3) or that independent of the Golgi has few recognized cargos and none of the regulators. In search of novel regulators for vacuolar trafficking routes and by using a fluorescence-based forward genetic screen, we demonstrated that the multispan transmembrane protein, Arabidopsis (Arabidopsis thaliana) PROTEIN S-ACYL TRANSFERASE10 (PAT10), is an AP-3-mediated vacuolar cargo...
July 2017: Plant Physiology
https://www.readbyqxmd.com/read/28553408/identification-of-cysteine-ubiquitylation-sites-on-the-sec23a-protein-of-the-copii-complex-required-for-vesicle-formation-from-the-er
#14
Giuseppina Amodio, Luigi Margarucci, Ornella Moltedo, Agostino Casapullo, Paolo Remondelli
BACKGROUND: COPII is a multiprotein complex that surrounds carrier vesicles budding from the Endoplasmic Reticulum and allows the recruitment of secretory proteins. The Sec23a protein plays a crucial role in the regulation of the dynamics of COPII formation ensuring the proper function of the secretory pathway. OBJECTIVE: Since few evidences suggest that ubiquitylation could have a role in the COPII regulation, the present study was aimed to establish whether the Sec23a component of the vesicular envelope COPII could be ubiquitylated...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28553287/hypoxia-modulates-the-response-of-mast-cells-to-staphylococcus-aureus-infection
#15
Helene Möllerherm, Katja Branitzki-Heinemann, Graham Brogden, Ayssar A Elamin, Wulf Oehlmann, Herbert Fuhrmann, Mahavir Singh, Hassan Y Naim, Maren von Köckritz-Blickwede
To study the antimicrobial function of immune cells ex vivo, cells are commonly cultivated under atmospheric oxygen concentrations (20-21%; normoxia), although the physiological oxygen conditions in vivo are significantly lower in most tissues. Especially during an acute infection, oxygen concentration locally decreases to hypoxic levels around or below 1%. The goal of this study was to investigate the effect of hypoxia on the activity of mast cells (MCs). MCs were cultivated for 3 or 24 h at 1% O2 in a hypoxia glove box and co-incubated with heat-inactivated Staphylococcus aureus...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28546447/signal-motifs-dependent-er-export-of-qc-snare-bet12-interacts-with-memb12-and-affects-pr1-trafficking-in-arabidopsis
#16
Kin Pan Chung, Yonglun Zeng, Yimin Li, Changyang Ji, Yiji Xia, Liwen Jiang
SNAREs are well-known for their role in controlling membrane fusion, the final but crucial step for vesicular transport in eukaryotes. SNARE proteins contribute to various biological processes including pathogen defense, channel activity regulation as well as plant growth and development. Precise targeting of SNARE proteins to destined compartments is a prerequisite for their proper functioning. However, the underlying mechanism(s) for SNAREs targeting in plants remains obscure. Here we investigate the targeting mechanism of the Qc-SNARE BET12, which is involved in protein trafficking in the early secretory pathway...
May 25, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28515296/hepatitis-c-virus-lipoviroparticles-hcv-lvp-assemble-in-the-endoplasmic-reticulum-er-and-bud-off-from-the-er-to-golgi-in-copii-vesicles
#17
Gulam H Syed, Mohsin Khan, Song Yang, Aleem Siddiqui
Hepatitis C virus (HCV) exists as a lipoprotein-virus hybrid lipoviroparticle (LVP). In vitro studies have demonstrated the importance of apolipoproteins in HCV secretion and infectivity leading to the notion that HCV co-opts the secretion of very-low density lipoprotein (VLDL) for its egress. However, our understanding of the mechanisms involved in virus particle assembly and egress, are still elusive. Biogenesis of VLDL particle occurs in the ER followed by subsequent lipidation in the ER and Golgi. Secretion of mature VLDL particles occurs through the Golgi secretory pathway...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28515233/a-wntless-sec12-complex-on-the-er-membrane-regulates-early-wnt-secretory-vesicle-assembly-and-mature-ligand-export
#18
Jiaxin Sun, Shiyan Yu, Xiao Zhang, Catherine Capac, Onyedikachi Aligbe, Timothy Daudelin, Edward M Bonder, Nan Gao
Wntless (Wls) transports Wnt molecules for secretion; however, the cellular mechanism underlying the initial assembly of Wnt secretory vesicles is still not fully defined. Here, we performed proteomic and mutagenic analyses of mammalian Wls, and report a mechanism for formation of early Wnt secretory vesicles on ER membrane. Wls forms a complex with SEC12 (also known as PREB), an ER membrane-localized guanine nucleotide-exchange factor (GEF) activator of the SAR1 (the SAR1A isoform) small GTPase. Compared to palmitoylation-deficient Wnt molecules, binding of mature Wnt to Wls increases Wls-SEC12 interaction and promotes association of Wls with SAR1, the key activator of the COPII machinery...
July 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28500182/upr-transducer-bbf2h7-allows-export-of-type-ii-collagen-in-a-cargo-and-developmental-stage-specific-manner
#19
Tokiro Ishikawa, Takuya Toyama, Yuki Nakamura, Kentaro Tamada, Hitomi Shimizu, Satoshi Ninagawa, Tetsuya Okada, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Eriko Aoyama, Masaharu Takigawa, Akihiro Harada, Kazutoshi Mori
The unfolded protein response (UPR) handles unfolded/misfolded proteins accumulated in the endoplasmic reticulum (ER). However, it is unclear how vertebrates correctly use the total of ten UPR transducers. We have found that ER stress occurs physiologically during early embryonic development in medaka fish and that the smooth alignment of notochord cells requires ATF6 as a UPR transducer, which induces ER chaperones for folding of type VIII (short-chain) collagen. After secretion of hedgehog for tissue patterning, notochord cells differentiate into sheath cells, which synthesize type II collagen...
June 5, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28499723/a-dual-site-gateway-cloning-system-for-simultaneous-cloning-of-two-genes-for-plant-transformation
#20
Mostafa Aboulela, Yuji Tanaka, Kohji Nishimura, Shoji Mano, Tetsuya Kimura, Tsuyoshi Nakagawa
Analyses of the subcellular localization of proteins and protein-protein interaction networks are essential to uncover the molecular basis of diverse biological processes in plants. To this end, we have created a Gateway cloning-compatible vector system, named dual-site (DS) Gateway cloning system to allow simple cloning of two expression cassettes in a binary vector and to express them simultaneously in plant cells. In the DS Gateway cloning system, (i) a moderate constitutive nopaline synthase promoter (Pnos), which is much suitable for localization analysis, is used to guide each expression cassette, (ii) four series of vectors with different plant resistance markers are established, (iii) N-terminal fusion with 6 fluorescent proteins and 7 epitope tags is available, (iv) both N- and C-terminal fusions with split enhanced yellow fluorescent protein (EYFP) are possible for efficient detection of protein-protein interactions using a bimolecular fluorescence complementation (BiFC) assay...
May 9, 2017: Plasmid
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