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https://www.readbyqxmd.com/read/28819300/yebc-a-putative-transcriptional-factor-involved-in-the-regulation-of-the-proteolytic-system-of-lactobacillus
#1
Lucía Brown, Josefina M Villegas, Mariano Elean, Silvina Fadda, Fernanda Mozzi, Lucila Saavedra, Elvira M Hebert
The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810950/clofibrate-attenuates-ros-production-by-lipid-overload-in-cultured-rat-hepatoma-cells
#2
Yufei Chen, Wei Li, Guqi Wang, Frank J Burczynski
PURPOSE: To investigate the effect of clofibrate on inducing liver fatty acid binding protein (FABP1) following a high-fat load in a hepatocyte cell culture model. METHODS: Rat hepatoma cells (CRL-1548) were treated with a fatty acid (FA) mixture consisting of oleate:palmitate (2:1) in the presence of 3% albumin. Cells were treated with 0, 0.5, 1, 2, or 3 mM FA for 24 and 48 hr, or further treated with 500 µM clofibrate (CLO) to induce FABP1 levels. Cytotoxicity was determined using the WST-1 assay...
2017: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28802844/structural-basis-for-cullins-and-ring-component-inhibition-targeting-e3-ubiquitin-pathway-conductors-for-cancer-therapeutics
#3
Shagufta Shafique, Waqar Ali, Sonia Kanwal, Sajid Rashid
Cullin (CUL)-RING E3 ubiquitin ligases (CRLs) are attractive therapeutic targets as they regulate diverse biological processes important for cancer cell survival by conferring substrate selectivity for ubiquitination and degradation. Given the complexity of CRL complexes, steps toward the structure-based design of small-molecule inhibitors to modulate their activity have remained elusive. In this study, we explored the structural assembly and interaction details of closely related CUL scaffolds (CUL1, CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7) with RBX1 to screen potent small molecules against CRLs...
August 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28802114/embryonic-and-fetal-morphology-in-the-lowland-paca-cuniculus-paca-a-precocial-hystricomorph-rodent
#4
Hani Rocha El Bizri, Frederico Ozanan Barros Monteiro, Rafael Dos Santos de Andrade, João Valsecchi, Diva Anelie de Araújo Guimarães, Pedro Mayor
In mammals, the embryonic and fetal development of a species has evolved to maximize neonatal survival. In this study, we use a sample of 132 embryos/fetuses of wild lowland paca (Cuniculus paca), obtained over a period of 15 years through collaborative methods with local hunters in the Amazon to describe the intrauterine development of external and internal morphology of this Neotropical rodent. We also compare the newborn survival strategy in this species with other rodents. The crown-rump length (CRL) ranged between 0...
August 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/28794045/pseudorabies-virus-dutpase-ul50-induces-lysosomal-degradation-of-type-i-interferon-receptor-1-and-antagonizes-the-alpha-interferon-response
#5
Rui Zhang, Aotian Xu, Chao Qin, Qiong Zhang, Shifan Chen, Yue Lang, Mengdong Wang, Chuang Li, Wenhai Feng, Rui Zhang, Zhengfan Jiang, Jun Tang
Alphaherpesviruses that establish persistent infection partly rely on their ability to evade host anti-viral responses, notably, the type I interferon (IFN) response. However, the mechanisms employed by alphaherpesviruses to avoid this response are not well understood. Pseudorabies virus (PRV) is an economically important pathogen and a useful model system to study alphaherpesvirus biology. To identify PRV proteins that antagonize type I IFN signaling, we performed a screen using an IFN-stimulated response element reporter in the swine cell line CRL...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28776111/methylene-blue-mediated-photobiomodulation-on-human-osteoblast-cells
#6
Gamze Bölükbaşı Ateş, Ayşe Ak, Bora Garipcan, Murat Gülsoy
Photobiomodulation (PBM) and photodynamic therapy (PDT) are two major methods, which use light in medicine and dentistry. PBM uses low-level laser light to induce cell proliferation and activity. In contrast, PDT use laser light combined with a photosensitizer (PS) to cause cell death. Due to similar, not fully understood mechanisms and biphasic response of light, unexpected and complex outcomes may be observed. In the present study, the effect of 635 nm laser light, with power density 50 mW/cm(2), at three different energy densities (0...
August 4, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28769978/ultrastructural-characteristics-of-rat-hepatic-oval-cells-and-their-intercellular-contacts-in-the-model-of-biliary-fibrosis-new-insights-into-experimental-liver-fibrogenesis
#7
Joanna Maria Lotowska, Maria Elzbieta Sobaniec-Lotowska, Dariusz Marek Lebensztejn, Urszula Daniluk, Piotr Sobaniec, Krzysztof Sendrowski, Jaroslaw Daniluk, Joanna Reszec, Wojciech Debek
PURPOSE: Recently, it has been emphasized that hepatic progenitor/oval cells (HPCs) are significantly involved in liver fibrogenesis. We evaluated the multipotential population of HPCs by transmission electron microscope (TEM), including relations with adherent hepatic nonparenchymal cells (NPCs) in rats with biliary fibrosis induced by bile duct ligation (BDL). METHODS: The study used 6-week-old Wistar Crl: WI(Han) rats after BDL for 1, 6, and 8 weeks. RESULTS: Current ultrastructural analysis showed considerable proliferation of HPCs in experimental intensive biliary fibrosis...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28764737/the-reproductive-pattern-and-potential-of-free-ranging-female-wild-boars-sus-scrofa-in-sweden
#8
Anna Malmsten, Gunnar Jansson, Nils Lundeheim, Anne-Marie Dalin
BACKGROUND: The number and spatial distribution of wild boars (Sus scrofa) has increased remarkably in Sweden as well as in other European countries. To understand the population dynamics of the wild boar, knowledge of its reproductive period, oestrus cycle and reproductive success is essential. The aim of this study was therefore to describe the seasonal reproductive pattern and reproductive potential of a wild boar population in Sweden. The study was based on findings from macroscopic examinations of the reproductive organs from 575 hunter-harvested female wild boars (>30 kg body weight)...
August 1, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28764542/mini-beam-modes-on-standard-mx-beamline-bl17u-at-ssrf
#9
Qisheng Wang, Feng Yu, Ying Cui, Kunhao Zhang, Qiangyan Pan, Changyou Zhong, Ke Liu, Huan Zhou, Bo Sun, Jianhua He
The macromolecular crystallography beamlines at third-generation synchrotron facilities play a central role in solving macromolecular crystal structures and also in understanding the biological function at molecular levels. The MX beamline BL17U at Shanghai Synchrotron Radiation Facility is a typical standard MX beamline with a focused beam size (H × V) of FWHM around 80 μm × 45 μm. However the protein samples brought to the beamline are down to 5-10 m from the important and challenging science project now...
July 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28753931/preparation-of-carriers-based-on-zno-nanoparticles-decorated-on-graphene-oxide-go-nanosheets-for-efficient-immobilization-of-lipase-from-candida-rugosa
#10
Shan Zhang, Jie Shi, Qianchun Deng, Mingming Zheng, Chuyun Wan, Chang Zheng, Ya Li, Fenghong Huang
Herein, a promising carrier, graphene oxide (GO) decorated with ZnO nanoparticles, denoted as GO/ZnO composite, has been designed and constructed. This carrier was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetry. Then, Candida rugosa lipase (CRL) was immobilized onto the GO-based materials via physical adsorption. Our results indicated that the lipase loading amount on the GO/ZnO composites was about 73.52 mg of protein per g...
July 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28752144/is-there-any-relationship-between-low-papp-a-levels-and-measures-of-umbilical-vein-and-placental-thickness-during-first-trimester-of-pregnancy
#11
Gulsum Uysal, Sadan Tutus, Fulya Cagli, Cevdet Adiguzel
OBJECTIVE: Low pregnancy-associated plasma protein A (PAPP-A) level is associated with adverse perinatal outcomes. The purpose of this study was to evaluate relationship between umbilical cord diameter (UCD), umbilical vein and artery diameters (UVD, UAD), placental thickness, and PAPP-A level at gestational age of between 11 and 14 weeks. METHODS: UCD, UVD, UAD, and placental thickness of 246 women were assessed during ultrasound examination at between 11 and 14 weeks of gestation, as well as measurement of nuchal translucency (NT) and crown-rump length (CRL)...
2017: Northern Clinics of Istanbul
https://www.readbyqxmd.com/read/28738156/coherent-random-lasing-from-dye-aggregates-in-polydimethylsiloxane-thin-films
#12
Lihua Ye, Yangyang Feng, Zhixiang Cheng, Chunlei Wang, Changgui Lu, Yanqing Lu, Yiping Cui
The coherent random laser (CRL) from dye-doped polydimethylsiloxane (PDMS) has been investigated in both nanoparticle-doped (NP-doped) thin films and pure dye thin films. Compared with the literature, the pump threshold is only 1.5 mJ/cm(2) in the pure dye thin film with a low dye concentration. The spontaneously formed micro-/nanocrystals of Pyrromethene 597 (PM597) dye support both gain and random feedback in the bulk of the PDMS during the sample preparation. When the SiO2 NPs were doped, the pump threshold was reduced to 0...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28733063/do-early-fetal-measurements-and-nuchal-translucency-correlate-with-term-birth-weight
#13
Rinat Hackmon, Clifford Librach, Richard Burwick, Nicole Rodrigues, Dan Farine, Howard Berger
OBJECTIVE: Traditionally, physiological variation in fetal weight is believed to emerge during the latter half of pregnancy. Although recent evidence suggests that crown-rump length (CRL) and nuchal translucency (NT) measured at 11-14 weeks correlate with abnormal fetal growth, findings have been limited by dating accuracy in spontaneous gestations. Therefore, we sought to determine whether CRL or NT measurements correlated with term birth weight (BW) or BW ratio in a cohort of IVF pregnancies, in which the date of conception is precisely known...
July 18, 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28732669/clinical-use-of-fetal-measurements-to-determine-the-whelping-day-in-german-shepherd-breed-bitches
#14
Marta Cecchetto, Chiara Milani, Juri Vencato, Hasan Sontas, Antonio Mollo, Barbara Contiero, Stefano Romagnoli
The aim of this work was to use a linear regression model previously developed in a pilot study to calculate days before parturition (DbP) using inner chorionic cavity (ICC), biparietal diameter (BPD), crown-rump length (CRL), body diameter (BD) and deep portion of telencephalic vesicle (DPTV) in German shepherd dogs (GSD) with known ovulation day and then to test that model in bitches with unknown ovulation day. In our current study, a model for GSD bitches published in a previous report, proved satisfactory for ICC [DbP=44...
July 12, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28732073/first-trimester-fetal-growth-restriction-and-the-occurrence-of-miscarriage-in-rural-bangladesh-a-prospective-cohort-study
#15
Harunor Rashid, Enbo Ma, Farzana Ferdous, Eva-Charlotte Ekström, Yukiko Wagatsuma
Fetal growth restriction in early pregnancy increases the risk of adverse pregnancy outcome, which has a significant social and psychological impact on women. There is limited information related to community-based study to evaluate early indicators related to miscarriage. The aim of this study is to examine the relationship between fetal growth restriction, measured by ultrasound crown-rump length (CRL), and subsequent occurrence of miscarriage in pregnant women in rural Bangladesh. The study was conducted within the Maternal and Infant Nutrition Interventions Trial in Matlab (MINIMat study), Bangladesh...
2017: PloS One
https://www.readbyqxmd.com/read/28719812/the-impact-of-zno-and-tio2-on-the-stability-of-clotrimazole-under-uva-irradiation-identification-of-photocatalytic-degradation-products-and-in-vitro-cytotoxicity-assessment
#16
Agata Kryczyk, Paweł Żmudzki, Paulina Koczurkiewicz, Joanna Piotrowska, Elżbieta Pękala, Urszula Hubicka
In order to ensure the safe and effective use of pharmaceutical products especially for topical administration photostability testing is necessary. The current paper presents an in-depth analysis of the stability of one of the most common antifungal agents, namely clotrimazole. Clotrimazole has proven to be stable under UVA irradiation in applied experimental conditions, but the presence of catalysts such as ZnO and TiO2 has contributed significantly to the degradation of this compound. The findings indicate that its photocatalytic degradation reactions followed the pseudo first-order kinetics with rate constant depending on the pH and the used solvent...
June 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28717191/mln4924-pevonedistat-a-protein-neddylation-inhibitor-suppresses-proliferation-and-migration-of-human-clear-cell-renal-cell-carcinoma
#17
Shuai Tong, Yang Si, Hefen Yu, Lingqiang Zhang, Ping Xie, Wenguo Jiang
Neddylation is a post-translational protein modification associated with cancer development. MLN4924 is a neddylation inhibitor currently under investigation in multiple phase I studies on various malignancies, and its clincal name is Pevonedistat. It has been documented that MLN4924 blocks Cullins neddylation and inactivates CRLs and, in turn, triggers cell-cycle arrest, apoptosis, senescence and autophagy in many cancer cells. In this study, we investigated the anti-tumor effect of MLN4924 in human clear cell renal carcinoma (ccRCC)...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716495/3-5-bis-3-dimethylaminomethyl-4-hydroxybenzylidene-4-piperidone-and-related-compounds-induce-glutathione-oxidation-and-mitochondria-mediated-cell-death-in-hct-116-colon-cancer-cells
#18
Eshwari Addala, Hossein Rafiei, Swagatika Das, Brian Bandy, Umashankar Das, Subhas S Karki, Jonathan R Dimmock
This study aims at investigating the cytotoxicity and some of the modes of action of 3,5-bis(3-dimethylamino-4-hydroxybenzylidene)-4-piperidone trihydrochloride 3 and two related compounds 2 (which lacks the dimethylaminomethyl groups) and 4 (which has an additional dimethylaminoethyl substituent in both aryl rings) in order to ascertain the contribution of dimethylaminoethyl substituent to bioactivity. The bioactivities of 2-4 were compared with curcumin 5. Both 2 and 3 displayed submicromolar GI50 values towards HCT-116 cells and were significantly more potent than 4, 5 and 5-fluorouracil (5-FU)...
July 5, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28707780/long-non-coding-rna-linc00261-inhibits-cell-growth-and-migration-in-endometriosis
#19
Lixiao Sha, Lingxiao Huang, Xishao Luo, Jiaping Bao, Lijun Gao, Qionghui Pan, Min Guo, Feiyun Zheng, Hanchu Wang
AIM: A previous study reported that LINC00261 is significantly downregulated in human ectopic endometrial tissues. The present study aimed to explore whether LINC00261 is functional in endometriosis cell proliferation, apoptosis and migration. METHODS: By transfecting human endometriosis cell line CRL-7566 with plasmids containing LINC00261, we successfully established the cell CRL-7566/LINC00261 with a high LINC00261 expression level. Cell-counting kit-8 and colony formation assays were conducted to evaluate the effect of LINC00261 on cell proliferation, and flow cytometry analysis and transwell migration assay were conducted to evaluate its effect on cell apoptosis and cell migration, respectively...
July 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28675343/state-of-the-art-in-the-validation-of-screening-methods-for-the-control-of-antibiotic-residues-is-there-a-need-for-further-development
#20
Valérie Gaudin
Screening methods are used as a first-line approach to detect the presence of antibiotic residues in food of animal origin. The validation process guarantees that the method is fit-for-purpose, suited to regulatory requirements, and provides evidence of its performance. This article is focused on intra-laboratory validation. The first step in validation is characterisation of performance, and the second step is the validation itself with regard to pre-established criteria. The validation approaches can be absolute (a single method) or relative (comparison of methods), overall (combination of several characteristics in one) or criterion-by-criterion...
July 4, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
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