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https://www.readbyqxmd.com/read/28423579/systematic-review-with-network-meta-analysis-comparative-efficacy-of-different-enteral-immunonutrition-formulas-in-patients-underwent-gastrectomy
#1
Guo-Min Song, Xiao-Ling Liu, Wei Bian, Jing Wu, Yong-Hong Deng, Hui Zhang, Xu Tian
OBJECTIVES: Optimal enteral immunonutrition (EIN) regime for gastric cancer (GC) patients underwent gastrectomy remains uncertainty. To assess comparative efficacy of different EIN formulas in GC patients underwent gastrectomy, we performed network meta-analysis. RESULTS: We included 11 RCTs enrolling 840 patients. Pairwise meta-analysis indicated that EIN (RR 0.56, 95% CI 0.36-0.86; MD -0.42, 95% CI -0.74-0.10), Arg+RNA+ω-3-FAs (RR 0.37, 95% CI 0.22-0.63; MD -0...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423032/complete-study-demonstrating-the-absence-of-rhabdovirus-in-a-distinct-sf9-cell-line
#2
Yoshifumi Hashimoto, Daniel Macri, Indresh Srivastava, Clifton McPherson, Rachael Felberbaum, Penny Post, Manon Cox
A putative novel rhabdovirus (SfRV) was previously identified in a Spodoptera frugiperda cell line (Sf9 cells [ATCC CRL-1711 lot 58078522]) by next generation sequencing and extensive bioinformatic analysis. We performed an extensive analysis of our Sf9 cell bank (ATCC CRL-1711 lot 5814 [Sf9L5814]) to determine whether this virus was already present in cells obtained from ATCC in 1987. Inverse PCR of DNA isolated from Sf9 L5814 cellular DNA revealed integration of SfRV sequences in the cellular genome. RT-PCR of total RNA showed a deletion of 320 nucleotides in the SfRV RNA that includes the transcriptional motifs for genes X and L...
2017: PloS One
https://www.readbyqxmd.com/read/28421190/first-trimester-crown-rump-length-and-embryonic-volume-of-fetuses-with-structural-congenital-abnormalities-measured-in-virtual-reality-an-observational-study
#3
L Baken, B Benoit, A H J Koning, P J van der Spek, E A P Steegers, N Exalto
Background. With the introduction of three-dimensional (3D) ultrasound it has become possible to measure volumes. The relative increase in embryonic volume (EV) is much larger than that of the crown-rump length (CRL) over the same time period. We examined whether EV is a better parameter to determine growth restriction in fetuses with structural congenital abnormalities. Study Design, Subjects, and Outcome Measures. CRL and EV were measured using a Virtual Reality (VR) system in prospectively collected 3D ultrasound volumes of 56 fetuses diagnosed with structural congenital abnormalities in the first trimester of pregnancy (gestational age 7(+5) to 14(+5) weeks)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28413162/whole-genome-sequence-of-the-metastatic-pc3-and-lncap-human-prostate-cancer-cell-lines
#4
Inge Seim, Penny L Jeffery, Patrick B Thomas, Colleen C Nelson, Lisa K Chopin
The bone metastasis-derived PC3 and the lymph node metastasis-derived LNCaP prostate cancer cell lines are widely studied, having been described in thousands of publications over the last four decades. Here, we report short-read whole-genome sequencing and de novo assembly of PC3 (ATCC CRL-1435) and LNCaP (clone FGC; ATCC CRL-1740) at ~70X coverage. A known homozygous mutation in TP53 and homozygous loss of PTEN were robustly identified in the PC3 cell line, whereas the LNCaP cell line exhibited a larger number of putative inactivating somatic point and indel mutations (and in particular loss of stop codon events)...
April 16, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28384153/cartilage-formation-in-the-pelvic-skeleton-during-the-embryonic-and-early-fetal-period
#5
Misaki Okumura, Aoi Ishikawa, Tomoki Aoyama, Shigehito Yamada, Chigako Uwabe, Hirohiko Imai, Tetsuya Matsuda, Akio Yoneyama, Tohoru Takeda, Tetsuya Takakuwa
The pelvic skeleton is formed via endochondral ossification. However, it is not known how the normal cartilage is formed before ossification occurs. Furthermore, the overall timeline of cartilage formation and the morphology of the cartilage in the pelvis are unclear. In this study, cartilage formation in the pelvic skeletons of 25 human fetuses (crown-rump length [CRL] = 11.9-75.0 mm) was observed using phase-contrast computed tomography and 7T magnetic resonance imaging. The chondrification center of the ilium, ischium, and pubis first appeared simultaneously at Carnegie stage (CS) 18, was located around the acetabulum, and grew radially in the later stage...
2017: PloS One
https://www.readbyqxmd.com/read/28383684/functional-variants-in-dcaf4-associated-with-lung-cancer-risk-in-european-populations
#6
Hongliang Liu, Zhensheng Liu, Yanru Wang, Thomas E Stinchcombe, Kouros Owzar, Younghun Han, Rayjean J Hung, Yonathan Brhane, John McLaughlin, Paul Brennan, Heike Bickeböller, Albert Rosenberger, Richard S Houlston, Neil Caporaso, Maria Teresa Landi, Irene Brüske, Angela Risch, Xifeng Wu, Yuanqing Ye, David C Christiani, Christopher I Amos, Qingyi Wei
Cullin-RING ubiquitin ligases (CRLs) responsible for substrate specificity of ubiquitination and play a key role in cell-cycle control and DNA damage response. In this study, we assessed associations between 16,599 SNPs in 115 CRL genes and lung cancer risk by using summary data of six published genome-wide association studies (GWASs) of 12,160 cases and 16,838 cases of European ancestry. As a result, we identified three independent SNPs in DCAF4 (rs117781739, rs12587742 and rs2240980) associated with lung cancer risk (odds ratio = 0...
April 5, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/28381314/effect-of-isoflurane-anesthesia-on-circadian-metabolism-and-physiology-in-rats
#7
Melissa A Wren-Dail, Robert T Dauchy, David E Blask, Steven M Hill, Tara G Ooms, Lynell M Dupepe, Rudolf P Bohm
Isoflurane anesthesia alters the blood levels of several neuroendocrine hormones associated with normal metabolism and physiology and increases stress, but the effect of brief CO2 anesthesia on these parameters is unknown. In this study, we examined the effects of isoflurane (4%) compared with brief CO2 (70% CO2, 30% air) anesthesia on circadian rhythms of plasma measures of physiology and metabolism. Adult male Sprague-Dawley rats (Crl:SD; n = 6 per group) were maintained on a 12:12-h light:dark (300 lx; lights on, 0600) photoperiod...
March 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28348718/intramyocardial-injection-of-recombinant-adeno-associated-viral-vector-coexpressing-pr39-adrenomedullin-enhances-angiogenesis-and-reduces-apoptosis-in-a-rat-myocardial-infarction-model
#8
Rui An, Cong Xi, Jian Xu, Ying Liu, Shumiao Zhang, Yuemin Wang, Yuewen Hao, Lijun Sun
Cotransfer of angiogenic and antiapoptotic genes could be the basis of new gene therapy strategies for myocardial infarction. In this study, rAAV-PR39-ADM, coexpressing antimicrobial peptide (PR39) and adrenomedullin (ADM), was designed with the mediation of recombinant adeno-associated virus. In vitro, CRL-1730 cells were divided into four groups, namely, the sham group, the AAV-null group, the NS (normal saline) group, and the PR39-ADM group. Immunocytochemistry analysis, CCK-8 assays, Matrigel assays, and apoptotic analysis were performed; in vivo, myocardial infarction model was established through ligation of the left coronary artery on rats, and treatment groups corresponded to those used in vitro...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28337869/knockdown-of-hmgb1-inhibits-cell-proliferation-and-induces-apoptosis-in-hemangioma-via-downregulation-of-akt-pathway
#9
C Pan, Y Wang, M K Qiu, S Q Wang, Y B Liu, Z W Quan, J M Ou
The high mobility group box 1 (HMGB1) as a conserved non-histone nuclear protein has been involved in a variety of biological processes of cancer, such as cell proliferation, apoptosis, angiogenesis and metastasis. Despite the increased expression of HMGB1 in many malignant tumors, the functions and molecular mechanisms by which HMGB1 contributes to the formation of hemangioma (HA) remain unclear. In the present study, immunohistochemistry was used to detect the expression levels of HMGB1 in different phases of human HAs...
January 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28333654/cytokine-changes-in-gastric-and-colonic-epithelial-cell-in-response-to-planta-ovata-extract
#10
Javed Yakoob, Wasim Jafri, Malik Hassan Mehmood, Zaigham Abbas, Kanwal Tariq
Background Psyllium (Planta ovata, Ispaghul) seed and husk are used for treatment of altered bowel habit, i. e. constipation and diarrhea. We studied the effect of Ispaghul extract on secretion of interleukin-1 beta (IL-1β) by AGS (ATCC CRL 1739) and SW480 (ATCC CCL-227) epithelial cell lines and determined whether Ispaghul extract has an effect on IL-1β secretion by Helicobacter pylori (H. pylori)-stimulated AGS cell and Escherichia coli K-12 (E. coli K-12)-stimulated SW480 cells in vitro. Methods The AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i...
March 22, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28323576/tat-rasgap317-326-enhances-radiosensitivity-of-human-carcinoma-cell-lines-in-vitro-and-in-vivo-through-promotion-of-delayed-mitotic-cell-death
#11
Pelagia Tsoutsou, Alessandro Annibaldi, David Viertl, Jonathan Ollivier, Franz Buchegger, Marie-Catherine Vozenin, Jean Bourhis, Christian Widmann, Oscar Matzinger
The synthetic peptide TAT-RasGAP317-326 has been shown to potentiate the efficacy of anti-cancer drugs. In this study, we explored the action of TAT-RasGAP317-326 when combined with radiation by investigating its radiosensitizing activity in vitro and in vivo. To investigate the modulation of intrinsic radiosensitivity induced by TAT-RasGAP317-326, clonogenic assays were performed using four human cancer cell lines, HCT116 p53(+/+) (ATCC: CCL-247), HCT116 p53(-/-), PANC-1 (ATCC: CRL-1469) and HeLa (ATCC: CCL-2), as well as one nontumor cell line, HaCaT (CLS: 300493)...
March 21, 2017: Radiation Research
https://www.readbyqxmd.com/read/28322186/effect-of-isoflurane-anesthesia-on-circadian-metabolism-and-physiology-in-rats
#12
Melissa A Wren-Dail Robert T Dauchy David E Blask Steven M Hill Tara G Ooms Lynell M Dupepe And Rudolf P Bohm
Isoflurane anesthesia alters the blood levels of several neuroendocrine hormones associated with normal metabolism andphysiology and increases stress, but the effect of brief CO2 anesthesia on these parameters is unknown. In this study, we examined the effects of isoflurane (4%) compared with brief CO2 (70% CO2, 30% air) anesthesia on circadian rhythms of plasma measures of physiology and metabolism. Adult male Sprague-Dawley rats (Crl:SD; n = 6 per group) were maintained on a 12:12-h light:dark(300 lx; lights on, 0600) photoperiod...
March 20, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28321044/investigation-of-the-early-response-genes-in-chemical-induced-renal-carcinogenicity-for-the-prediction-of-chemical-carcinogenicity-in-rats
#13
Hiroshi Matsumoto, Fumiyo Saito, Masahiro Takeyoshi
This study was designed to identify early-response genes of chemical-induced renal carcinogenicity for the prediction of chemical carcinogenicity in rats. We conducted a 28-day repeated-dose test in male Crl:CD (SD) rats with 12 carcinogens and 10 non-carcinogens as the training dataset, and five carcinogens and five non-carcinogens as the validation dataset. Renal gene expression profiles were analyzed by using a microarray. Fifteen candidate genes were selected from the gene expression profiles of the training dataset as genes that showed specific expression in response to carcinogens...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28316180/-effect-and-related-mechanism-of-microrna-181-attenuates-oxidized-low-density-lipoprotein-induced-vascular-endothelial-cell-injury
#14
N N Wang, X Sun, X L Zhang, L Lou, K M Chen, H Li, L Tang, W G Wang, M Zhang
Objective: To observe the expression level of microRNA-181 (miR-181) and importin-α3 in oxidized low density lipoprotein (ox-LDL) induced vascular endothelial cell injury models, and explore the effect and mechanism of miR-181 on endothelial cell injury. Methods: Human vein endothelial cell line CRL-1730 were cultured and vascular endothelial cell injury model was established by intervention with ox-LDL. The cells were divided into control group (intervened by double distilled water), low-dose group (intervened by 10 μg/ml ox-LDL) and high-dose group (intervened by 20 μg/ml ox-LDL)...
March 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28294371/icariin-improves-the-sexual-function-of-male-mice-through-the-pi3k-akt-enos-no-signalling-pathway
#15
J Ding, Y Tang, Z Tang, X Zu, L Qi, X Zhang, G Wang
We aimed to investigate the effect and mechanism of icariin on male sexual function. Forty-eight Crl:CD1(ICR) male mice were randomly divided into control, low-, medium- and high-dose icariin group (intragastric administration of 50, 100 and 200 mg/kg/d for 21 days). Mating experiment was then performed at a ratio of 1: 3 (male: female). The mating behaviours of male mice were recorded. The genital indexes and serum testosterone, nitric oxide (NO), hypothalamic dopamine (DA) and 5- hydroxy tryptophan (5-HT) concentrations were measured...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28292554/selected-lactobacilli-and-bifidobacteria-development-in-solid-state-fermentation-using-soybean-paste
#16
Antonieta Rodríguez de Olmos, Maria Alejandra Correa Deza, Marisa S Garro
At present, consumers are looking for more natural foods so as to improve health through their active compounds. Within this context, soybean is an excellent substrate due to its beneficial effects on consumers' health. Moreover, lactic cultures are widely used in the food industry to improve the technological, nutritional and functional characteristics of fermented foods. It is interesting to find new matrices in which to transport these starter cultures (potentially probiotic microorganisms). The aim of this research was to obtain a solid state fermentation system from soybean to analyze the behavior of selected lactobacilli and bifidobacteria, with the potential to develop a functional vegetarian food to serve as carrier for the microorganisms...
March 12, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28280842/in-vitro-effect-of-vaginal-lactobacilli-on-the-growth-and-adhesion-abilities-of-uropathogenic-escherichia-coli
#17
María Cecilia Leccese Terraf, María Silvina Juarez Tomás, Lucie Rault, Yves Le Loir, Sergine Even, María Elena Fátima Nader-Macías
Escherichia coli is one of the main causes of uncomplicated urinary tract infections and responsible of vaginal infections. Lactobacilli can inhibit this pathogen by the production of antimicrobial substances as organic acids, hydrogen peroxide and/or bacteriocins. The aim of this work was to study the effects of beneficial vaginal lactobacilli on E. coli through in vitro experiments. The inhibitory activity of three vaginal Lactobacillus strains against E. coli was assessed using the agar plate diffusion. Moreover, the effect of Lactobacillus reuteri CRL (Centro de Referencia para Lactobacilos Culture Collection) 1324 on the adhesion and internalization capabilities of E...
March 9, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28265745/investigation-of-deactivation-thermodynamics-of-lipase-immobilized-on-polymeric-carrier
#18
Kirtikumar C Badgujar, Bhalchandra M Bhanage
In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0...
March 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28254558/early-serum-biomarker-networks-in-infants-with-distinct-retinochoroidal-lesion-status-of-congenital-toxoplasmosis
#19
Thádia Evelyn de Araújo, Jordana Grazziela Coelho-Dos-Reis, Samantha Ribeiro Béla, Ana Carolina Aguiar Vasconcelos Carneiro, Anderson Silva Machado, Ludmila Melo Cardoso, Ágata Lopes Ribeiro, Michelle Hallais França Dias, Gláucia Manzan Queiroz Andrade, Daniel Vitor Vasconcelos-Santos, José Nélio Januário, Andréa Teixeira-Carvalho, Ricardo Wagner Almeida Vitor, Eloisa Amália Vieira Ferro, Olindo Assis Martins-Filho
The present study characterized the early changes in the serum chemokines/cytokine signatures and networks in infants with congenital-toxoplasmosis/(TOXO) as compared to non-infected-controls/(NI). TOXO were subgrouped according to the retinochoroidal lesion status as no-lesion/(NL), active-lesion/(ARL), active/cicatricial-lesion/(ACRL) and cicatricial-lesion/(CRL). The results showed that TOXO display prominent chemokine production mediated by IL-8/CXCL8, MIG/CXCL9, IP-10/CXCL10 and RANTES/CCL5. Additionally, TOXO is accompanied by mixed proinflammatory/regulatory cytokine pattern mediated by IL-6, IFN-γ, IL-4, IL-5 and IL-10...
February 28, 2017: Cytokine
https://www.readbyqxmd.com/read/28253196/cytotoxicity-and-cytochrome-p450-inhibitory-activities-of-clinacanthus-nutans
#20
Suk Yen Quah, Jin Han Chin, Gabriel Akyirem Akowuah, Shaik Ibrahim Khalivulla, Siew Wei Yeong, Mandumpal Chacko Sabu
BACKGROUND: Clinacanthus nutans Lindau (family: Acanthaceae), also known as "Sabah Snake Grass" or "Belalai Gajah" in Malaysia, has been widely used by Malaysians due to its anticancer property. However, the anticancer activity of C. nutans leaves extract and its safe use need to be further investigated. The objectives of the present study were to evaluate the cytotoxic effects of methanol leaves extract of C. nutans in various human cancer cell lines and to evaluate the in vitro effect of C...
March 1, 2017: Drug Metabolism and Personalized Therapy
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