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https://www.readbyqxmd.com/read/28333654/cytokine-changes-in-gastric-and-colonic-epithelial-cell-in-response-to-planta-ovata-extract
#1
Javed Yakoob, Wasim Jafri, Malik Hassan Mehmood, Zaigham Abbas, Kanwal Tariq
Background Psyllium (Planta ovata, Ispaghul) seed and husk are used for treatment of altered bowel habit, i. e. constipation and diarrhea. We studied the effect of Ispaghul extract on secretion of interleukin-1 beta (IL-1β) by AGS (ATCC CRL 1739) and SW480 (ATCC CCL-227) epithelial cell lines and determined whether Ispaghul extract has an effect on IL-1β secretion by Helicobacter pylori (H. pylori)-stimulated AGS cell and Escherichia coli K-12 (E. coli K-12)-stimulated SW480 cells in vitro. Methods The AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i...
March 22, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28323576/tat-rasgap317-326-enhances-radiosensitivity-of-human-carcinoma-cell-lines-in-vitro-and-in-vivo-through-promotion-of-delayed-mitotic-cell-death
#2
Pelagia Tsoutsou, Alessandro Annibaldi, David Viertl, Jonathan Ollivier, Franz Buchegger, Marie-Catherine Vozenin, Jean Bourhis, Christian Widmann, Oscar Matzinger
The synthetic peptide TAT-RasGAP317-326 has been shown to potentiate the efficacy of anti-cancer drugs. In this study, we explored the action of TAT-RasGAP317-326 when combined with radiation by investigating its radiosensitizing activity in vitro and in vivo. To investigate the modulation of intrinsic radiosensitivity induced by TAT-RasGAP317-326, clonogenic assays were performed using four human cancer cell lines, HCT116 p53(+/+) (ATCC: CCL-247), HCT116 p53(-/-), PANC-1 (ATCC: CRL-1469) and HeLa (ATCC: CCL-2), as well as one nontumor cell line, HaCaT (CLS: 300493)...
March 21, 2017: Radiation Research
https://www.readbyqxmd.com/read/28322186/effect-of-isoflurane-anesthesia-on-circadian-metabolism-and-physiology-in-rats
#3
Melissa A Wren-Dail Robert T Dauchy David E Blask Steven M Hill Tara G Ooms Lynell M Dupepe And Rudolf P Bohm
Isoflurane anesthesia alters the blood levels of several neuroendocrine hormones associated with normal metabolism andphysiology and increases stress, but the effect of brief CO2 anesthesia on these parameters is unknown. In this study, we examined the effects of isoflurane (4%) compared with brief CO2 (70% CO2, 30% air) anesthesia on circadian rhythms of plasma measures of physiology and metabolism. Adult male Sprague-Dawley rats (Crl:SD; n = 6 per group) were maintained on a 12:12-h light:dark(300 lx; lights on, 0600) photoperiod...
March 20, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28321044/investigation-of-the-early-response-genes-in-chemical-induced-renal-carcinogenicity-for-the-prediction-of-chemical-carcinogenicity-in-rats
#4
Hiroshi Matsumoto, Fumiyo Saito, Masahiro Takeyoshi
This study was designed to identify early-response genes of chemical-induced renal carcinogenicity for the prediction of chemical carcinogenicity in rats. We conducted a 28-day repeated-dose test in male Crl:CD (SD) rats with 12 carcinogens and 10 non-carcinogens as the training dataset, and five carcinogens and five non-carcinogens as the validation dataset. Renal gene expression profiles were analyzed by using a microarray. Fifteen candidate genes were selected from the gene expression profiles of the training dataset as genes that showed specific expression in response to carcinogens...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28316180/-effect-and-related-mechanism-of-microrna-181-attenuates-oxidized-low-density-lipoprotein-induced-vascular-endothelial-cell-injury
#5
N N Wang, X Sun, X L Zhang, L Lou, K M Chen, H Li, L Tang, W G Wang, M Zhang
Objective: To observe the expression level of microRNA-181 (miR-181) and importin-α3 in oxidized low density lipoprotein (ox-LDL) induced vascular endothelial cell injury models, and explore the effect and mechanism of miR-181 on endothelial cell injury. Methods: Human vein endothelial cell line CRL-1730 were cultured and vascular endothelial cell injury model was established by intervention with ox-LDL. The cells were divided into control group (intervened by double distilled water), low-dose group (intervened by 10 μg/ml ox-LDL) and high-dose group (intervened by 20 μg/ml ox-LDL)...
March 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28294371/icariin-improves-the-sexual-function-of-male-mice-through-the-pi3k-akt-enos-no-signalling-pathway
#6
J Ding, Y Tang, Z Tang, X Zu, L Qi, X Zhang, G Wang
We aimed to investigate the effect and mechanism of icariin on male sexual function. Forty-eight Crl:CD1(ICR) male mice were randomly divided into control, low-, medium- and high-dose icariin group (intragastric administration of 50, 100 and 200 mg/kg/d for 21 days). Mating experiment was then performed at a ratio of 1: 3 (male: female). The mating behaviours of male mice were recorded. The genital indexes and serum testosterone, nitric oxide (NO), hypothalamic dopamine (DA) and 5- hydroxy tryptophan (5-HT) concentrations were measured...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28292554/selected-lactobacilli-and-bifidobacteria-development-in-solid-state-fermentation-using-soybean-paste
#7
Antonieta Rodríguez de Olmos, Maria Alejandra Correa Deza, Marisa S Garro
At present, consumers are looking for more natural foods so as to improve health through their active compounds. Within this context, soybean is an excellent substrate due to its beneficial effects on consumers' health. Moreover, lactic cultures are widely used in the food industry to improve the technological, nutritional and functional characteristics of fermented foods. It is interesting to find new matrices in which to transport these starter cultures (potentially probiotic microorganisms). The aim of this research was to obtain a solid state fermentation system from soybean to analyze the behavior of selected lactobacilli and bifidobacteria, with the potential to develop a functional vegetarian food to serve as carrier for the microorganisms...
March 12, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28280842/in-vitro-effect-of-vaginal-lactobacilli-on-the-growth-and-adhesion-abilities-of-uropathogenic-escherichia-coli
#8
María Cecilia Leccese Terraf, María Silvina Juarez Tomás, Lucie Rault, Yves Le Loir, Sergine Even, María Elena Fátima Nader-Macías
Escherichia coli is one of the main causes of uncomplicated urinary tract infections and responsible of vaginal infections. Lactobacilli can inhibit this pathogen by the production of antimicrobial substances as organic acids, hydrogen peroxide and/or bacteriocins. The aim of this work was to study the effects of beneficial vaginal lactobacilli on E. coli through in vitro experiments. The inhibitory activity of three vaginal Lactobacillus strains against E. coli was assessed using the agar plate diffusion. Moreover, the effect of Lactobacillus reuteri CRL (Centro de Referencia para Lactobacilos Culture Collection) 1324 on the adhesion and internalization capabilities of E...
March 9, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28265745/investigation-of-deactivation-thermodynamics-of-lipase-immobilized-on-polymeric-carrier
#9
Kirtikumar C Badgujar, Bhalchandra M Bhanage
In the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. Immobilization of lipase Candida rugosa (CRL) was carried out on biocompatible blend of poly vinyl alcohol (PVA) and chitosan (CHY) support via entrapment and glutardehyde (Glu) cross-linking method to produce PVA:CHY:CRL and PVA:CHY:Glu:CRL as robust biocatalyst. These immobilized lipases were characterized by various physico-biochemical characterization techniques. Later on, thermal and solvent stability of polymer immobilized lipase was determined in term of half-life time (t 0...
March 6, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28254558/early-serum-biomarker-networks-in-infants-with-distinct-retinochoroidal-lesion-status-of-congenital-toxoplasmosis
#10
Thádia Evelyn de Araújo, Jordana Grazziela Coelho-Dos-Reis, Samantha Ribeiro Béla, Ana Carolina Aguiar Vasconcelos Carneiro, Anderson Silva Machado, Ludmila Melo Cardoso, Ágata Lopes Ribeiro, Michelle Hallais França Dias, Gláucia Manzan Queiroz Andrade, Daniel Vitor Vasconcelos-Santos, José Nélio Januário, Andréa Teixeira-Carvalho, Ricardo Wagner Almeida Vitor, Eloisa Amália Vieira Ferro, Olindo Assis Martins-Filho
The present study characterized the early changes in the serum chemokines/cytokine signatures and networks in infants with congenital-toxoplasmosis/(TOXO) as compared to non-infected-controls/(NI). TOXO were subgrouped according to the retinochoroidal lesion status as no-lesion/(NL), active-lesion/(ARL), active/cicatricial-lesion/(ACRL) and cicatricial-lesion/(CRL). The results showed that TOXO display prominent chemokine production mediated by IL-8/CXCL8, MIG/CXCL9, IP-10/CXCL10 and RANTES/CCL5. Additionally, TOXO is accompanied by mixed proinflammatory/regulatory cytokine pattern mediated by IL-6, IFN-γ, IL-4, IL-5 and IL-10...
February 28, 2017: Cytokine
https://www.readbyqxmd.com/read/28253196/cytotoxicity-and-cytochrome-p450-inhibitory-activities-of-clinacanthus-nutans
#11
Suk Yen Quah, Jin Han Chin, Gabriel Akyirem Akowuah, Shaik Ibrahim Khalivulla, Siew Wei Yeong, Mandumpal Chacko Sabu
BACKGROUND: Clinacanthus nutans Lindau (family: Acanthaceae), also known as "Sabah Snake Grass" or "Belalai Gajah" in Malaysia, has been widely used by Malaysians due to its anticancer property. However, the anticancer activity of C. nutans leaves extract and its safe use need to be further investigated. The objectives of the present study were to evaluate the cytotoxic effects of methanol leaves extract of C. nutans in various human cancer cell lines and to evaluate the in vitro effect of C...
March 1, 2017: Drug Metabolism and Personalized Therapy
https://www.readbyqxmd.com/read/28245560/the-regulations-of-deubiquitinase-usp15-and-its-pathophysiological-mechanisms-in-diseases
#12
REVIEW
Chon-Kit Chou, Yu-Ting Chang, Michal Korinek, Yei-Tsung Chen, Ya-Ting Yang, Steve Leu, I-Ling Lin, Chin-Ju Tang, Chien-Chih Chiu
Deubiquitinases (DUBs) play a critical role in ubiquitin-directed signaling by catalytically removing the ubiquitin from substrate proteins. Ubiquitin-specific protease 15 (USP15), a member of the largest subfamily of cysteine protease DUBs, contains two conservative cysteine (Cys) and histidine (His) boxes. USP15 harbors two zinc-binding motifs that are essential for recognition of poly-ubiquitin chains. USP15 is grouped into the same category with USP4 and USP11 due to high degree of homology in an N-terminal region consisting of domains present in ubiquitin-specific proteases (DUSP) domain and ubiquitin-like (UBL) domain...
February 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28244432/simulating-and-optimizing-compound-refractive-lens-based-x-ray-microscopes
#13
Hugh Simons, Sonja Rosenlund Ahl, Henning Friis Poulsen, Carsten Detlefs
A comprehensive optical description of compound refractive lenses (CRLs) in condensing and full-field X-ray microscopy applications is presented. The formalism extends ray-transfer matrix analysis by accounting for X-ray attenuation by the lens material. Closed analytical expressions for critical imaging parameters such as numerical aperture, spatial acceptance (vignetting), chromatic aberration and focal length are provided for both thin- and thick-lens imaging geometries. These expressions show that the numerical aperture will be maximized and chromatic aberration will be minimized at the thick-lens limit...
March 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28230778/caffeic-acid-expands-anti-tumor-effect-of-metformin-in-human-metastatic-cervical-carcinoma-htb-34-cells-implications-of-ampk-activation-and-impairment-of-fatty-acids-de-novo-biosynthesis
#14
Malgorzata Tyszka-Czochara, Pawel Konieczny, Marcin Majka
The efficacy of cancer treatments is often limited and associated with substantial toxicity. Appropriate combination of drug targeting specific mechanisms may regulate metabolism of tumor cells to reduce cancer cell growth and to improve survival. Therefore, we investigated the effects of anti-diabetic drug Metformin (Met) and a natural compound caffeic acid (trans-3,4-dihydroxycinnamic acid, CA) alone and in combination to treat an aggressive metastatic human cervical HTB-34 (ATCC CRL-1550) cancer cell line...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28229932/csn5-jab1-suppresses-the-wnt-inhibitor-dkk1-in-colorectal-cancer-cells
#15
Sandra Jumpertz, Thomas Hennes, Yaw Asare, Anke K Schütz, Jürgen Bernhagen
The COP9 signalosome (CSN) is a multi-protein complex that is highly conserved in eukaryotes. Due to its regulatory impact on processes such as cell cycle, DNA damage response and apoptosis, the CSN is essential for mammalian cells. One of the best-studied functions of the CSN is the deNEDDylation of cullin-RING ligases (CRLs) via its catalytically active subunit CSN5/JAB1, thereby triggering the degradation of various target proteins. CSN5 was found to be overexpressed in many human cancer entities, including colon adenocarcinoma...
February 14, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28222836/presumptive-spontaneous-lysosomal-storage-like-disease-in-a-crl-cd1-icr-mouse
#16
Krista M Hernon, Tiffany L Whitcomb, Lori Davis, Timothy K Cooper
A clinically unremarkable 4.5-mo-old female Crl:CD1(ICR) VAF/Elite mouse was euthanized for scheduled sentinel processing. Gross necropsy findings included significant hepatosplenomegaly and visceral lymphadenomegaly, resulting in a preliminary gross diagnosis of lymphoma. Histology revealed florid accumulations of large, 'foamy' macrophages present in the bone marrow, small intestines, and viscera including liver, spleen, lymph nodes, thymus, uterus, and ovaries. The cytoplasm of these cells was abundant, stained pale blue with Wright-Giemsa and was periodic acid-Schiff positive...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28181320/are-foetal-ultrasonographic-and-maternal-blood-progesterone-measurements-near-parturition-reliable-predictors-of-the-time-of-birth-in-the-domestic-cat
#17
R Keiser, I M Reichler, O Balogh
In cats, accuracy of parturition day prediction by ultrasonographic measurement of foetal structures is decreasing towards the end of gestation. Foetal measurements during the last days of pregnancy are scarce. We determined foetal biparietal, abdominal and eye diameter (BPD, AD and ED, respectively) by ultrasonography as well as maternal blood progesterone (P4) within five days of delivery to predict parturition date and calculate accuracy of prediction. Foetal BPD at birth was compared with newborn kitten head diameter (HD)...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28166272/activation-of-mapk-erk-signaling-by-burkholderia-pseudomallei-cycle-inhibiting-factor-cif
#18
Mei Ying Ng, Mei Wang, Patrick J Casey, Yunn-Hwen Gan, Thilo Hagen
Cycle inhibiting factors (Cifs) are virulence proteins secreted by the type III secretion system of some Gram-negative pathogenic bacteria including Burkholderia pseudomallei. Cif is known to function to deamidate Nedd8, leading to inhibition of Cullin E3 ubiquitin ligases (CRL) and consequently induction of cell cycle arrest. Here we show that Cif can function as a potent activator of MAPK/ERK signaling without significant activation of other signaling pathways downstream of receptor tyrosine kinases. Importantly, we found that the ability of Cif to activate ERK is dependent on its deamidase activity, but independent of Cullin E3 ligase inhibition...
2017: PloS One
https://www.readbyqxmd.com/read/28164322/reference-ranges-of-placental-volume-measured-by-virtual-organ-computer-aided-analysis-between-10-and-14-weeks-of-gestation
#19
Daranee Meengeonthong, Suchaya Luewan, Supatra Sirichotiyakul, Theera Tongsong
OBJECTIVE: To establish the reference ranges of the placental volume between 10 and 14 weeks of gestation of Thai fetuses. METHODS: The placental volumes were acquired in normal pregnancies between 10 and 14 weeks of gestation using transabdominal three-dimensional ultrasound. The placental volume was then analyzed using VOCAL (virtual organ computer-aided analysis) technique with a rotation angle of 30°. The measured values were regressed to identify the best-fit model...
February 6, 2017: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/28161414/dairy-propionibacteria-prevent-the-proliferative-effect-of-plant-lectins-on-sw480%C3%A2-cells-and-protect-the-metabolic-activity-of-the-intestinal-microbiota-in%C3%A2-vitro
#20
Gabriela Zárate, Gabriel D Sáez, Adriana Pérez Chaia
Plant lectins are specific carbohydrate-binding proteins that are widespread in legumes such as beans and pulses, seeds, cereals, and many plants used as farm feeds. They are highly resistant to cooking and digestion, reaching the intestinal lumen and/or blood circulation with biological activity. Since many legume lectins trigger harmful local and systemic reactions after their binding to the mucosal surface, these molecules are generally considered anti-nutritive and/or toxic substances. In the gut, specific cell receptors and bacteria may interact with these dietary components, leading to changes in intestinal physiology...
February 1, 2017: Anaerobe
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