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https://www.readbyqxmd.com/read/28622784/first-trimester-inter-and-intrafetal-size-discrepancies-in-bichorionic-twins-conceived-by-in%C3%A2-vitro-fertilization-can-it-predict-pregnancy-outcome
#1
Ron Bardin, Galia Oron, Yael Levy, Onit Sapir, Israel Meizner, Benjamin Fisch, Arnon Wiznitzer, Eran Hadar
OBJECTIVE: To evaluate the association between first-trimester inter- and intrafetal size discrepancies and pregnancy outcome, among bichorionic-biamniotic twins conceived by IVF. DESIGN: A retrospective study design was used. SETTING: Tertiary university-affiliated medical center. PATIENT(S): All women with a viable first-trimester bichorionic-biamniotic twin gestation, who conceived after IVF in 2007-2015. INTERVENTION(S): None...
June 13, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28620047/sccro-neddylates-cul3-to-selectively-promote-midbody-localization-and-activity-of-cul3klhl21-during-abscission
#2
Guochang Huang, Andrew J Kaufman, Ke Xu, Katia Manova, Bhuvanesh Singh
SCCRO/DCUN1D1, a component of the neddylation E3 complex, regulates the activity of the cullin-RING-ligase (CRL) type of ubiquitination E3s by promoting neddylation of cullin family members. Studies have shown SCCRO regulates proliferation in vitro and in vivo. Here, we show that inactivation of SCCRO results in prolonged mitotic time due to delayed and/or failed abscission. The effects of SCCRO on abscission involve its role in neddylation and localization of Cul3 to the midbody. The Cul3 adaptor KLHL21 mediates SCCRO's effects on abscission, as it fails to localize to the midbody in SCCRO-deficient cells during abscission and its inactivation resulted in phenotypic changes identical to SCCRO inactivation...
June 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28618747/first-trimester-ultrasonographic-parameters-in-prediction-of-the-course-and-outcome-of-monochorionic-twin-pregnancies
#3
Slavica Aksam, Snezana Plesinac, Jelena Dotlic, Jasmina Tadic, Svetlana Vrzic-Petronijevic, Milos Petronijevic, Dusica Kocijancic-Belovic, Snezana Buzadzic
BACKGROUND/AIM: The aim of the study was to evaluate the association and the potential predictive value of first trimester ultrasonographic parameters on the course and outcome of monochorionic diamniotic twin pregnancies. MATERIALS AND METHODS: A prospective cohort study was undertaken of 39 healthy women with consecutive monochorionic diamniotic twin pregnancies. During first-trimester screening, crown-rump length (CRL) and nuchal translucency (NT) were measured...
June 12, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28604823/the-chromosome-analysis-of-the-miscarriage-tissue-miscarried-embryo-fetal-crown-rump-length-crl-measurement-a-practical-use
#4
Silvia D'ippolito, Nicoletta Di Simone, Daniela Orteschi, Maria Grazia Pomponi, Maurizio Genuardi, Leuconoe Grazia Sisti, Roberta Castellani, Esther Diana Rossi, Giovanni Scambia, Marcella Zollino
OBJECTIVE: To investigate whether miscarried embryo/fetal crown rump length (CRL) measurement may yield a practical application for predicting a conclusive result at the cytogenetic analysis of miscarriage tissue. Our study might help in improving the cytogenetic method, the results of which may be affected by maternal cell contamination (MCC). In particular, we aimed at establishing whether the miscarried embryo/fetal CRL measurement shows accuracy in predicting the possibility of MCC and the scan cut-off value useful to this purpose and, as a result, suggest a multi-step procedure for the genetic ascertainment...
2017: PloS One
https://www.readbyqxmd.com/read/28602043/efficacy-of-first-trimester-ultrasound-parameters-for-prediction-of-early-spontaneous-abortion
#5
Mamta Rath Datta, Ankush Raut
OBJECTIVE: To assess first-trimester ultrasound measurements for the prediction of early spontaneous abortion. METHODS: In a prospective observational study in Jamshedpur, India, women with a singleton pregnancy of 42-76 days were enrolled between November 2014 and April 2016. Inclusion criteria were spontaneous conception, embryonic cardiac activity, and regular menstrual cycle. Fetal crown-to-rump length (CRL), gestational sac diameter (GSD), yolk sac diameter (YSD), and fetal heart rate (FHR) were measured by transvaginal ultrasonography...
June 11, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28596679/oxidative-stress-induced-mitochondrial-dysfunction-in-a-normal-colon-epithelial-cell-line
#6
Nandakumar Packiriswamy, Kari F Coulson, Susan J Holcombe, Lorraine M Sordillo
AIM: To determine how a normal human colon cell line reacts to microbial challenge as a way to study oxidative stress-induced responses associated with inflammatory bowel disease. METHODS: Normal human colon epithelial cells (ATCC(®) CRL.1790™) were stimulated with either heat killed E. coli or heat killed murine cecal contents (HKC) and examined for several relevant biomarkers associated with inflammation and oxidative stress including cytokine production, mitochondrial autophagy and oxidant status...
May 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28594955/inorganic-salts-and-intracellular-polyphosphate-inclusions-play-a-role-in-the-thermotolerance-of-the-immunobiotic-lactobacillus-rhamnosus-crl-1505
#7
María A Correa Deza, Mariana Grillo-Puertas, Susana Salva, Viviana A Rapisarda, Carla L Gerez, Graciela Font de Valdez
In this work, the thermotolerance of Lactobacillus rhamnosus CRL1505, an immunobiotic strain, was studied as a way to improve the tolerance of the strain to industrial processes involving heat stress. The strain displayed a high intrinsic thermotolerance (55°C, 20 min); however, after 5 min at 60°C in phosphate buffer a two log units decrease in cell viability was observed. Different heat shock media were tested to improve the cell survival. Best results were obtained in the mediumcontaining inorganic salts (KH2PO4, Na2HPO4, MnSO4, and MgSO4) likely as using 10% skim milk...
2017: PloS One
https://www.readbyqxmd.com/read/28591624/crystal-structure-of-the-cul2-rbx1-elobc-vhl-ubiquitin-ligase-complex
#8
Teresa A F Cardote, Morgan S Gadd, Alessio Ciulli
Cullin RING E3 ubiquitin ligases (CRLs) function in the ubiquitin proteasome system to catalyze the transfer of ubiquitin from E2 conjugating enzymes to specific substrate proteins. CRLs are large dynamic complexes and attractive drug targets for the development of small-molecule inhibitors and chemical inducers of protein degradation. The atomic details of whole CRL assembly and interactions that dictate subunit specificity remain elusive. Here we present the crystal structure of a pentameric CRL2(VHL) complex, composed of Cul2, Rbx1, Elongin B, Elongin C, and pVHL...
June 6, 2017: Structure
https://www.readbyqxmd.com/read/28590004/chemoenzymatic-synthesis-of-optically-active-phenolic-3-4-dihydropyridin-2-ones-a-way-to-access-enantioenriched-1-4-dihydropyridine-and-benzodiazepine-derivatives
#9
Susana Y Torres, Rosario Brieva, Francisca Rebolledo
A chemoenzymatic approach for the synthesis of optically active 4-(3-acetoxyphenyl)-5-(alkoxycarbonyl)-6-methyl-3,4-dihydropyridin-2-ones (3,4-DHP-2-ones) and their hydroxyphenyl derivatives has been developed, the key step being a Candida rugosa lipase (CRL)-catalyzed hydrolysis reaction. As a result, different optically active 3,4-DHP-2-ones have been prepared with very high enantiomeric excesses (ee = 94-99%) and good yields. The enantioenriched 3,4-DHP-2-ones have easily been converted into highly functionalized (R)- and (S)-1,4-dihydropyridines (1,4-DHPs) by means of a Vilsmeier-Haack reaction...
June 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28588674/resveratrol-ameliorates-diabetic-nephropathy-in-rats-through-negative-regulation-of-the-p38-mapk-tgf-%C3%AE-1-pathway
#10
Yuan Qiao, Ke Gao, Yangwei Wang, Xueliang Wang, Bo Cui
Resveratrol (RSV) has been shown to have a renoprotective effect against diabetic nephropathy, but the underlying mechanisms of this have not been fully elucidated. The aim of the current study was to explore the mechanisms responsible for the therapeutic effects of RSV in rat mesangial cells in vitro and in a rat model of diabetic nephropathy. The viability of CRL-2573 rat mesangial cells and their expression levels of p38, phosphorylated (p)-p38, transforming growth factor beta 1 (TGF-β1) and fibronectin were assessed in response to treatment with high glucose, with or without RSV...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28585900/strategies-for-the-screening-of-antibiotic-residues-in-eggs-comparison-of-the-validation-of-classical-microbiological-method-with-an-immunobiosensor-method
#11
Valérie Gaudin, Annie Rault, Celine Hedou, Christophe Soumet, Eric Verdon
Efficient screening methods are needed to control antibiotic residues in eggs. A microbiological kit (Explorer® 2.0 test (Zeu Inmunotech, Spain)) and an immunobiosensor kit (Microarray II (AM® II) on the Evidence Investigator™ system (Randox, UK)) have been evaluated and validated for screening of antibiotic residues in eggs, according to Decision EC/2002/657 (2002) and to the European guideline for the validation of screening methods (Crl 2010). The e-reader™ system, a new automatic incubator/reading system, was coupled to the Explorer® 2...
June 6, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28583667/dietary-safety-of-a-dual-enzyme-preparation-for-animal-feed-acute-and-subchronic-oral-toxicity-and-genotoxicity-studies
#12
G P Dillon, M A Gaffney, C M Curran, C A Moran
Animal feed is routinely supplemented with exogenous enzymes to improve nutrient utilization, such as proteases to enhance protein hydrolysis in vivo and xylanases to alleviate feed related anti-nutritional factors. The present studies were conducted to evaluate the potential oral toxicity and genotoxicity of a dual-enzyme preparation, Vegpro(®) concentrate (VPr-C). Acute oral toxicity studies were conducted in adult male and female Sprague-Dawley Crl CD rats and CHS Swiss ICO:OFI (IOPS Caw) mice. Thirteen week preliminary and final subchronic oral toxicity studies were conducted in male and female rats...
June 3, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28580429/specific-targeting-of-the-deubiquitinase-and-e3-ligase-families-with-engineered-ubiquitin-variants
#13
REVIEW
Maryna Gorelik, Sachdev S Sidhu
The ubiquitin proteasome system (UPS) has garnered much attention due to its potential for the development of therapeutics. Following a successful clinical application of general proteasome inhibitors much effort has been devoted to targeting individual UPS components including E3 enzymes and deubiquitinases that control specificity of ubiquitination. Our group has developed a novel approach for targeting the UPS proteins using engineered ubiquitin variants (Ubvs). These drug-like proteins can serve as valuable tools to study biological function of UPS components and assist in the development of small molecules for clinical use...
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/28575008/plasma-metabolome-and-skin-proteins-in-charcot-marie-tooth-1a-patients
#14
Beatriz Soldevilla, Carmen Cuevas-Martín, Clara Ibáñez, Fulvio Santacatterina, María A Alberti, Carolina Simó, Carlos Casasnovas, Celedonio Márquez-Infante, Teresa Sevilla, Samuel I Pascual, María Sánchez-Aragó, Carmen Espinos, Francesc Palau, José M Cuezva
OBJECTIVE: Charcot-Marie-Tooth 1A (CMT1A) disease is the most common inherited neuropathy that lacks of therapy and of molecular markers to assess disease severity. Herein, we have pursued the identification of potential biomarkers in plasma samples and skin biopsies that could define the phenotype of CMT1A patients at mild (Mi), moderate (Mo) and severe (Se) stages of disease as assessed by the CMT neuropathy score to contribute to the understanding of CMT pathophysiology and eventually inform of the severity of the disease...
2017: PloS One
https://www.readbyqxmd.com/read/28567021/assessment-of-fetal-development-using-cardiac-valve-intervals
#15
Faezeh Marzbanrad, Ahsan H Khandoker, Yoshitaka Kimura, Marimuthu Palaniswami, Gari D Clifford
An automated method to assess the fetal physiological development is introduced which uses the component intervals between fetal cardiac valve timings and the Q-wave of fetal electrocardiogram (fECG). These intervals were estimated automatically from one-dimensional Doppler Ultrasound and noninvasive fECG. We hypothesize that the fetal growth can be estimated by the cardiac valve intervals. This hypothesis was evaluated by modeling the fetal development using the cardiac intervals and validating against the gold standard gestational age identified by Crown-Rump Length (CRL)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28554384/synthesis-of-fibrous-and-non-fibrous-mesoporous-silica-magnetic-yolk-shell-microspheres-as-recyclable-supports-for-immobilization-of-candida-rugosa-lipase
#16
Zafar Ali, Lei Tian, Baoliang Zhang, Nisar Ali, Muhammad Khan, Qiuyu Zhang
The mesoporous yolk-shell nanoparticles (MYSNs), with a movable Fe3O4 core inside the hollow capsules, with two different morphologies (1) Fe3O4@SiO2@hollow@fibrous SiO2 (Yolk Shell-1) (2) Fe3O4@SiO2@hollow@mesoporousSiO2 (Yolk Shell-2) were fabricated as carriers for Candida rugosa lipase (CRL) immobilization, through a template-assistant selectively etching method. Firstly the hydrophilic Fe3O4 nanoparticles were synthesized according to the solvothermal method. Then, SiO2 shell was coated on the Fe3O4 nanoparticle surface by a sol-gel reaction...
August 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28553771/the-first-trimester-combined-test-for-aneuploidies-a-single-center-experience
#17
Alina Veduta, Ana Maria Vayna, Simona Duta, Anca Panaitescu, Florin Popescu, Maria Bari, Gheorghe Peltecu, Florina Nedelea
PURPOSE: We present the results of the systematic application of the first trimester combined test for aneuploidies, in a Romanian center. METHODS: Since October 2009, in Filantropia Hospital in Bucharest, we have systematically been using the FMF (Fetal Medicine Foundation) combined first trimester test to screen for common aneuploidies at 11 to 13 + 6 weeks of gestation. We assessed the crown - rump length (CRL), nuchal translucency, fetal heart rate as well as PAPP-A and free β-hCG in maternal serum...
May 28, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28549792/endocannabinoids-modulate-apoptosis-in-endometriosis-and-adenomyosis
#18
Elif Bilgic, Elif Guzel Meydanli, Sevil Kose, Makbule Cisel Aydin, Eda Karaismailoglu, Irem Akar, Alp Usubutun, Petek Korkusuz
Adenomyosis that is a form of endometriosis is the growth of ectopic endometrial tissue within the muscular wall of the uterus (myometrium), which may cause dysmenorrhea and infertility. Endocannabinoid mediated apoptotic mechanisms of endometriosis and adenomyosis are not known. We hypothesized that the down regulation of endocannabinoid receptors and/or alteration in their regulatory enzymes may have a direct role in the pathogenesis of endometriosis and adenomyosis through apoptosis. Endocannabinoid receptors CB1 and CB2, their synthesizing and catabolizing enzymes (FAAH, NAPE-PLD, DAGL, MAGL) and the apoptotic indexes were immunohistochemically assessed in endometriotic and adenomyotic tissues...
May 23, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28547130/extracellular-dopamine-acetylcholine-and-activation-of-dopamine-d1-and-d2-receptors-after-selective-breeding-for-cocaine-self-administration-in-rats
#19
Haiyang Xu, Sasmita Das, Marc Sturgill, Colin Hodgkinson, Qiaoping Yuan, David Goldman, Kenneth Grasing
RATIONALE: The low self-administration (LS)/Kgras (LS) and high self-administration (HS)/Kgras (HS) rat lines were generated by selective breeding for low- and high-intravenous cocaine self-administration, respectively, from a common outbred Wistar stock (Crl:WI). This trait has remained stable after 13 generations of breeding. OBJECTIVE: The objective of the present study is to compare cocaine preference, neurotransmitter release, and dopamine receptor activation in LS and HS rats...
May 26, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28546788/dmd-transcripts-in-crl-2061-rhabdomyosarcoma-cells-show-high-levels-of-intron-retention-by-intron-specific-pcr-amplification
#20
Emma Tabe Eko Niba, Ryo Yamanaka, Abdul Qawee Mahyoob Rani, Hiroyuki Awano, Masaaki Matsumoto, Hisahide Nishio, Masafumi Matsuo
BACKGROUND: The DMD gene encoding dystrophin is mutated in Duchenne muscular dystrophy, a fatal progressive muscle wasting disease. DMD has also been shown to act as a tumor suppressor gene. Rhabdomyosarcoma (RMS) is a mesodermal sarcoma that shares characteristics of skeletal muscle precursors. Products of the DMD gene in RMS have not yet been fully clarified. Here, DMD products were analyzed in CRL-2061 cells established from alveolar RMS. METHODS: The 14-kb long DMD cDNA was PCR amplified as 20 separated fragments, as were nine short intron regions...
2017: Cancer Cell International
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