Katalin Todorov-Völgyi, Judit González-Gallego, Stephan A Müller, Nathalie Beaufort, Rainer Malik, Martina Schifferer, Mihail Ivilinov Todorov, Dennis Crusius, Sophie Robinson, Andree Schmidt, Jakob Körbelin, Florence Bareyre, Ali Ertürk, Christian Haass, Mikael Simons, Dominik Paquet, Stefan F Lichtenthaler, Martin Dichgans
Age-related decline in brain endothelial cell (BEC) function contributes critically to neurological disease. Comprehensive atlases of the BEC transcriptome have become available, but results from proteomic profiling are lacking. To gain insights into endothelial pathways affected by aging, we developed a magnetic-activated cell sorting-based mouse BEC enrichment protocol compatible with proteomics and resolved the profiles of protein abundance changes during aging. Unsupervised cluster analysis revealed a segregation of age-related protein dynamics with biological functions, including a downregulation of vesicle-mediated transport...
March 22, 2024: Nature aging