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Methylation

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https://www.readbyqxmd.com/read/28107736/crystal-structures-binding-interactions-and-adme-evaluation-of-brain-penetrant-n-substituted-indazole-5-carboxamides-as-subnanomolar-selective-monoamine-oxidase-b-and-dual-mao-a-b-inhibitors
#1
Nikolay T Tzvetkov, Hans-Georg Stammler, Beate Neumann, Silvia Hristova, Liudmil Antonov, Marcus Gastreich
The pharmacological and physicochemical analysis of structurally optimized N-alkyl-substituted indazole-5-carboxamides, developed as potential drug and radioligand candidates for the treatment and diagnosis of Parkinson's disease (PD) and other neurological disorders, is reported. Recent efforts have been focused on the development of subnanomolar potent, selective MAO-B (N1-alkyl-substituted compounds 12a-14a and 15) and dual active MAO-A/B (N2-methylated compounds 12b-14b) inhibitors with nanomolar potency towards MAO-B and moderately active against MAO-A enzyme, respectively...
January 11, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28107709/quantification-of-fatty-acids-in-the-muscle-of-antarctic-fish-trematomus-bernacchii-by-gas-chromatography-mass-spectrometry-optimization-of-the-analytical-methodology
#2
C Truzzi, S Illuminati, A Annibaldi, M Antonucci, G Scarponi
This work presents data on the quantification of fatty acids (FAs, in terms of mass unit per tissue weight) in the muscle of Trematomus bernacchii, a key species in Antarctica, often used as bioindicator for contamination studies. Modifications in fatty acids content should be considered a useful biomarker to study how contaminants affect Antarctic biota. Until now, very few studies quantified fatty acids of muscle of T. bernacchii, and only as percentage of a single fatty acid on total lipids. To perform the quantification of fatty acids, we used an analytical method based on a fast microwave-assisted extraction of lipids from a lyophilized sample, a base-catalyzed trans-esterification of lipid extract to obtain Fatty Acids Methyl Esters (FAMEs), and a separation and identification of FAMEs by gas chromatography-mass spectrometry...
December 31, 2016: Chemosphere
https://www.readbyqxmd.com/read/28107650/acetylation-enhances-tet2-function-in-protecting-against-abnormal-dna-methylation-during-oxidative-stress
#3
Yang W Zhang, Zhihong Wang, Wenbing Xie, Yi Cai, Limin Xia, Hariharan Easwaran, Jianjun Luo, Ray-Whay Chiu Yen, Yana Li, Stephen B Baylin
TET proteins, by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), are hypothesized, but not directly shown, to protect promoter CpG islands (CGIs) against abnormal DNA methylation (DNAm) in cancer. We define such a protective role linked to DNA damage from oxidative stress (OS) known to induce this abnormality. TET2 removes aberrant DNAm during OS through interacting with DNA methyltransferases (DNMTs) in a "Yin-Yang" complex targeted to chromatin and enhanced by p300 mediated TET2 acetylation...
January 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28107644/synergism-of-dam-muth-and-muts-in-methylation-directed-mismatch-repair-in-escherichia-coli
#4
Changkun Hu, Yunqi Zhao, Huiyun Sun, Yixin Yang
DNA mismatch repair (MMR) is a critical mutation surveillance system for recognizing and repairing erroneous insertion, deletion, and disincorporation of base. Major components of mismatch repair system consist of MutH, MutL, and MutS. Dam methylates adenine to distinguish newly synthesized daughter strands from the parent strands. Employing a tyrosine-auxotrophic E. coli FX-11 strain, the mutation frequency can be determined by the number of tyrosine revertants and the cell viability of FX-11 with deficiencies in dam and mismatch repair proteins...
January 6, 2017: Mutation Research
https://www.readbyqxmd.com/read/28107630/2-o-methyl-5-hydroxymethylcytidine-a-second-oxidative-derivative-of-5-methylcytidine-in-rna
#5
Sabrina M Huber, Pieter van Delft, Arun A Tanpure, Eric A Miska, Shankar Balasubramanian
5-hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopomers and high-resolution tandem LC-MS/MS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms, revealed varying levels and TET-independent formation of this new RNA modification.
January 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28107589/serum-lincrna-p21-as-a-potential-biomarker-of-liver-fibrosis-in-chronic-hepatitis-b-patients
#6
Fujun Yu, Guangyao Zhou, Kate Huang, XuFei Fan, Guojun Li, Bicheng Chen, Peihong Dong, Jianjian Zheng
Serum long non-coding RNAs (lncRNAs) are emerging as promising biomarkers for various human diseases. The aim of this study was to investigate the feasibility of using serum long intergenic non-coding RNA-p21 (lincRNA-p21) as a biomarker for chronic hepatitis B patients. Serum lincRNA-p21 levels were quantified using real-time PCR in 417 CHB patients and 363 healthy controls. The promoter methylation level of lincRNA-p21 was detected using bisulfite-sequencing analysis in primary hepatic stellate cells (HSCs)...
January 20, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28107587/p450-bm3-catalyzed-regio-and-stereoselective-hydroxylation-aiming-at-the-synthesis-of-phthalides-and-isocoumarins
#7
Claudia Holec, Ute Hartrampf, Katharina Neufeld, Joerg Pietruszka
Cytochrome P450 BM3 monooxygenases are able to catalyze the regio- and stereoselective oxygenation of a broad range of substrates with promising potential for synthetic applications. To study the ability of P450 BM3 variants for the stereoselective benzylic hydroxylation of 2-alkylated benzoic acid esters, the biotransformation of methyl 2-ethylbenzoate was investigated resulting in both enantiomeric forms of 3-methylphthalide. In case of methyl 2-propylbenzoate as a substrate the regioselectivity of the reaction was shifted towards -hydroxylation resulting in the synthesis of enantioenriched (R)- and (S)-configured 3-methylisochroman-1-one...
January 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28107536/correction-methylation-markers-of-early-stage-non-small-cell-lung-cancer
#8
Kaie Lokk, Tõnu Vooder, Raivo Kolde, Kristjan Välk, Urmo Võsa, Retlav Roosipuu, Lili Milani, Krista Fischer, Marina Koltsina, Egon Urgard, Tarmo Annilo, Andres Metspalu, Neeme Tõnisson
[This corrects the article DOI: 10.1371/journal.pone.0039813.].
2017: PloS One
https://www.readbyqxmd.com/read/28107465/lead-exposure-induces-weight-gain-in-adult-rats-accompanied-by-dna-hypermethylation
#9
Honglin Sun, Ningjian Wang, Xiaomin Nie, Li Zhao, Qin Li, Zhen Cang, Chi Chen, Meng Lu, Jing Cheng, Hualing Zhai, Fangzhen Xia, Lin Ye, Yingli Lu
OBJECTIVE: Previous studies have revealed the association of lead (Pb) exposure with obesity. DNA methylation alteration has been suggested to be one of the regulatory mechanisms of obesity. We aimed to explore whether Pb exposure is related with weight gain and DNA methylation alteration. METHODS: Male adult 8 week Wistar rats were divided into 5 groups: the normal chow diet (NCD); the NCD+0.05%Pb; the NCD+0.15%Pb; the NCD+0.45%Pb and the high fat diet. Rats were exposed to different dosages of Pb through drinking water for 21 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28107440/new-composites-lnbdc-ac-and-cb-6-ac-from-design-toward-selective-adsorption-of-methylene-blue-or-methyl-orange
#10
Guilherme de C Santos, Amanda L Barros, Carlos A F de Oliveira, Leonis L da Luz, Fausthon F da Silva, Grégoire J-F Demets, Severino Alves Júnior
New porous composites LnBDC@AC (AC = Activated carbon, Ln = Eu and Gd and BDC = 1,4-benzenedicaboxylate) and CB[6]@AC (CB[6] = Cucurbit[6]uril) were obtained using hydrothermal route. The LnBDC and CB[B] are located inside the pore of the carbon materials as was observed in SEM-EDS, XRPD and FT-IR analysis. Porosimetry analysis showed values typically between AC and LnBDC material, with pore size and surface area, respectively, 29,56 Å and 353.98 m2g-1 for LnBDC@AC and 35,53 Å and 353.98 m2g-1 for CB[6]@AC...
2017: PloS One
https://www.readbyqxmd.com/read/28107365/parenclitic-network-analysis-of-methylation-data-for-cancer-identification
#11
Alexander Karsakov, Thomas Bartlett, Artem Ryblov, Iosif Meyerov, Mikhail Ivanchenko, Alexey Zaikin
We make use of ideas from the theory of complex networks to implement a machine learning classification of human DNA methylation data, that carry signatures of cancer development. The data were obtained from patients with various kinds of cancers and represented as parenclictic networks, wherein nodes correspond to genes, and edges are weighted according to pairwise variation from control group subjects. We demonstrate that for the 10 types of cancer under study, it is possible to obtain a high performance of binary classification between cancer-positive and negative samples based on network measures...
2017: PloS One
https://www.readbyqxmd.com/read/28107360/correction-methyl-farnesoate-plays-a-dual-role-in-regulating-drosophila-metamorphosis
#12
Di Wen, Crisalejandra Rivera-Perez, Mohamed Abdou, Qiangqiang Jia, Qianyu He, Xi Liu, Ola Zyaan, Jingjing Xu, William G Bendena, Stephen S Tobe, Fernando G Noriega, Subba R Palli, Jian Wang, Sheng Li
[This corrects the article DOI: 10.1371/journal.pgen.1005038.].
January 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28107179/nuclear-prmt5-cyclin-d1-and-il-6-are-associated-with-poor-outcome-in-oropharyngeal-squamous-cell-carcinoma-patients-and-is-inversely-associated-with-p16-status
#13
Bhavna Kumar, Arti Yadav, Nicole V Brown, Songzhu Zhao, Michael J Cipolla, Paul E Wakely, Alessandra C Schmitt, Robert A Baiocchi, Theodoros N Teknos, Matthew Old, Pawan Kumar
Protein arginine methyltransferase-5 (PRMT5) plays an important role in cancer progression by repressing the expression of key tumor suppressor genes via the methylation of transcriptional factors and chromatin-associated proteins. However, very little is known about the expression and biological role of PRMT5 in head and neck cancer. In this study, we examined expression profile of PRMT5 at subcellular levels in oropharyngeal squamous cell carcinoma (OPSCC) and assessed its correlation with disease progression and patient outcome...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28107178/influence-of-structure-of-lactones-with-the-methylcyclohexene-and-dimethylcyclohexene-ring-on-their-biotransformation-and-antimicrobial-activity
#14
Katarzyna Wińska, Małgorzata Grabarczyk, Wanda Mączka, Barbara Żarowska, Gabriela Maciejewska, Mirosław Anioł
The aim of this article is influence of the structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. This work was based on the general remark that even the smallest change in the structure of a compound can affect its biological properties. The results of the biotransformation of four bicyclic unsaturated lactones with one or two methyl groups in the cyclohexene ring was tested using fifteen fungal strains (Fusarium species, Penicillium species, Absidia species, Cunninghamella japonica, and Pleurotus ostreatus) and five yeast strains (Yarrowia lipolytica, Rhodorula marina, Rhodorula rubra, Candida viswanathii, and Saccharomyces cerevisiae)...
January 20, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28107177/antinociceptive-effect-of-aristolochia-trilobata-stem-essential-oil-and-6-methyl-5-hepten-2yl-acetate-its-main-compound-in-rodents
#15
Jullyana de Souza Siqueira Quintans, Rafael Dos Santos Alves, Darlisson de Alexandria Santos, Mairim Russo Serafini, Péricles Barreto Alves, Emmanoel Vilaça Costa, Gokhan Zengin, Lucindo José Quintans-Júnior, Adriana Gibara Guimarães
Aristolochia trilobata L. is an aromatic plant, popularly known as "mil-homens", and its essential oil (EO) is generally used to treat colic, diarrhea and dysentery disorders. We evaluated the antinociceptive effect of A. trilobata stem EO and of its major compound, the (R)-(-)-6-methyl-5-hepten-2-yl acetate (sulcatyl acetate: SA), using acetic acid (0.85%)-induced writhing response and formalin-induced (20 μL of 1%) nociceptive behavior in mice. We also evaluated the EO and SA effect on motor coordination, using the rota-rod apparatus...
January 20, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28106993/mild-aliphatic-and-benzylic-hydrocarbon-c-h-bond-chlorination-using-trichloroisocyanuric-acid-tcca
#16
Sascha H Combe, Abolfazl Hosseini, Alejandro Parra, Peter R Schreiner
We present the controlled monochlorination of aliphatic and benzylic hydrocarbons with only one equivalent of substrate at 25-30 °C using N-hydroxyphthalimide (NHPI) as radical initiator and commercially available trichloroisocyanuric acid (TCCA) as the chlorine source. Catalytic amounts of CBr4 reduced the reaction times considerably due to the formation of chain-carrying •CBr3 radicals. Benzylic C-H chlorination affords moderate to good yields for arenes carrying electron-withdrawing (50-85%) or weakly electron-donating groups (31-73%); cyclic aliphatic substrates provide low yields (24-38%)...
January 20, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28106943/a-pilot-investigation-of-the-impact-of-smoking-cessation-on-biological-age
#17
Man-Kit Lei, Steven R H Beach, Meeshanthini V Dogan, Robert A Philibert
BACKGROUND AND OBJECTIVES: Smoking is known to increase biological age. However, whether this process is reversible through smoking cessation is not known. In this pilot study, we attempt to determine whether smoking cessation reduces biological age. METHODS: We conducted regression analyses of methylation data from 22 subjects, as they entered and exited inpatient substance use treatment, to determine change in biological age, as indicated by the deviation of their methylomic age from chronological age across two time points...
January 20, 2017: American Journal on Addictions
https://www.readbyqxmd.com/read/28106929/conformational-analysis-of-an-antibacterial-cyclodepsipeptide-active-against-m-tuberculosis-by-a-combined-roe-and-rdc-analysis
#18
Maic Fredersdorf, Michael Kurz, Armin Bauer, Marc-Olivier Ebert, Carla Rigling, Laurie Lannes, Christina Thiele
Griselimycin (GM) and methylgriselimycin (MGM), naturally produced by microorganisms of the genus Streptomyces, are cyclic depsipeptides composed of ten amino acids. They exhibit antibacterial activity against mycobacterium species by inhibiting the sliding clamp of prokaryotic DNA polymerase III and are therefore considered as potential anti-tuberculosis drugs. The difference between both peptides is the presence of L -(R)-4-methylproline in MGM instead of L -proline in GM at position 8 of the amino acid sequence...
January 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28106918/divergent-cytosine-dna-methylation-patterns-in-single-cell-soybean-root-hairs
#19
Md Shakhawat Hossain, Taiji Kawakatsu, Kyung Do Kim, Zhang Ning, Cuong T Nguyen, Saad M Khan, Josef M Batek, Trupti Joshi, Jeremy Schmutz, Jane Grimwood, Robert J Schmitz, Dong Xu, Scott A Jackson, Joseph R Ecker, Gary Stacey
Chromatin modifications, such as cytosine methylation of DNA, play a significant role in mediating gene expression in plants, which affects growth, development, and cell differentiation. As root hairs are single-cell extensions of the root epidermis and the primary organs for water uptake and nutrients, we sought to use root hairs as a single-cell model system to measure the impact of environmental stress. We measured changes in cytosine DNA methylation in single-cell root hairs as compared with multicellular stripped roots, as well as in response to heat stress...
January 20, 2017: New Phytologist
https://www.readbyqxmd.com/read/28106826/colorectal-carcinoma-a-general-overview-and-future-perspectives-in-colorectal-cancer
#20
REVIEW
Inés Mármol, Cristina Sánchez-de-Diego, Alberto Pradilla Dieste, Elena Cerrada, María Jesús Rodriguez Yoldi
Colorectal cancer (CRC) is the third most common cancer and the fourth most common cause of cancer-related death. Most cases of CRC are detected in Western countries, with its incidence increasing year by year. The probability of suffering from colorectal cancer is about 4%-5% and the risk for developing CRC is associated with personal features or habits such as age, chronic disease history and lifestyle. In this context, the gut microbiota has a relevant role, and dysbiosis situations can induce colonic carcinogenesis through a chronic inflammation mechanism...
January 19, 2017: International Journal of Molecular Sciences
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