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major vault protein

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https://www.readbyqxmd.com/read/27817195/mechanisms-underlying-lung-resistance-related-protein-lrp-mediated-doxorubicin-resistance-of-non-small-cell-lung-cancer-cells
#1
Yu-Lun Chen, Tsung-Ying Yang, Chieh-Liang Wu, Kun-Chieh Chen, Shih-Lan Hsu, Chi-Mei Hsueh
Lung resistance-related protein (LRP) is a human major vault protein (MVP) implicated in drug resistance of cancer cells in a cell-type dependent manner. The primary goal of the study was to understand the role(s) of LRP in doxorubicin (DOX) resistance of non-small cell lung cancer (NSCLC) cells and the underlying working mechanisms. In the study, the roles of LRP in the regulation of DOX dynamics, nuclear import of minor vault proteins (vPARP and TEP1) and DOX-mediated cytotoxicity were examined in CH27 and H460 cells...
December 31, 2016: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/27275002/major-vault-protein-in-cardiac-and-smooth-muscle
#2
Nataliia V Shults, Dividutta Das, Yuichiro J Suzuki
Major vault protein (MVP) is the major component of the vault particle whose functions are not well understood. One proposed function of the vault is to serve as a mechanism of drug transport, which confers drug resistance in cancer cells. We show that MVP can be found in cardiac and smooth muscle. In human airway smooth muscle cells, knocking down MVP was found to cause cell death, suggesting that MVP serves as a cell survival factor. Further, our laboratory found that MVP is S-glutathionylated in response to ligand/receptor-mediated cell signaling...
2016: Receptors & Clinical Investigation
https://www.readbyqxmd.com/read/27165595/-effects-of-cyclooxygenase-2-selective-inhibitor-celecoxib-on-the-expression-of-major-vault-protein-in-rats-with-status-epilepticus
#3
Ting-Ting Song, Dan Li, Shao-Ping Huang, Lin Yang, Xue-Ying Wang, Yong-Sheng Jiang, Yu Liu
OBJECTIVE: To study the effect of cyclooxygenase -2 selective inhibitor celecoxib on the expression of major vault protein ( MVP) in the brain of rats with status epilepticus and its possible roles in the treatment of refractory epilepsy. METHODS: Sixty adult male Sprague-Dawley rats were randomly assigned to blank control (n=16), epilepsy model (n=22) and celecoxib treatment groups (n=22). After the status epilepticus was induced in rats by injecting lithium and pilocarpine, each group had 16 rats enrolled as subjects...
May 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27073578/association-of-single-nucleotide-polymorphisms-in-the-mvp-gene-with-platinum-resistance-and-survival-in-patients-with-epithelial-ovarian-cancer
#4
Ya-Nan Zhao, Dong-Ning He, Ya-DI Wang, Jun-Jie Li, Min-Wen Ha
The human major vault protein (MVP) has been linked to the development of multidrug resistance in cancer cells, and overexpression of MVP has been observed in ovarian cancer tissues. The aim of the present study was to investigate the association between single nucleotide polymorphisms (SNPs) in the MVP gene and the tumor response to platinum-based chemotherapy and survival of patients affected by epithelial ovarian cancer (EOC), in addition to confirm whether tetra-primer amplification-refractory mutation system (ARMS)-polymerase chain reaction (PCR) is an accurate genotyping method...
April 2016: Oncology Letters
https://www.readbyqxmd.com/read/26982031/analysis-of-gene-expression-in-3d-spheroids-highlights-a-survival-role-for-ass1-in-mesothelioma
#5
Dario Barbone, Loes Van Dam, Carlo Follo, Puthen V Jithesh, Shu-Dong Zhang, William G Richards, Raphael Bueno, Dean A Fennell, V Courtney Broaddus
To investigate the underlying causes of chemoresistance in malignant pleural mesothelioma, we have studied mesothelioma cell lines as 3D spheroids, which acquire increased chemoresistance compared to 2D monolayers. We asked whether the gene expression of 3D spheroids would reveal mechanisms of resistance. To address this, we measured gene expression of three mesothelioma cell lines, M28, REN and VAMT, grown as 2D monolayers and 3D spheroids. A total of 209 genes were differentially expressed in common by the three cell lines in 3D (138 upregulated and 71 downregulated), although a clear resistance pathway was not apparent...
2016: PloS One
https://www.readbyqxmd.com/read/26952044/carfilzomib-reverses-pulmonary-arterial-hypertension
#6
Xinhong Wang, Yasmine F Ibrahim, Dividutta Das, Makhosazane Zungu-Edmondson, Nataliia V Shults, Yuichiro J Suzuki
AIMS: Pulmonary arterial hypertension (PAH) remains a lethal disease with pronounced narrowing of pulmonary vessels due to abnormal cell growth. Agents that can reduce the pulmonary vascular thickness thus have therapeutic potential. The present study investigated the efficacy of carfilzomib (CFZ), a proteasome inhibitor and a cancer chemotherapeutic drug, on reversing PAH. METHODS AND RESULTS: In two rat models of PAH, SU5416/hypoxia and SU5416/ovalbumin, CFZ effectively reversed pulmonary vascular remodelling with the promotion of apoptosis and autophagy...
May 15, 2016: Cardiovascular Research
https://www.readbyqxmd.com/read/26843330/inducible-major-vault-protein-plays-a-pivotal-role-in-double-stranded-rna-or-virus-induced-proinflammatory-response
#7
Nanfang Peng, Shi Liu, Zhangchuan Xia, Sheng Ren, Jian Feng, Mingzhen Jing, Xin Gao, Erik A C Wiemer, Ying Zhu
Pathogen invasion triggers robust antiviral cytokine production via different transcription factor signaling pathways. We have previously demonstrated that major vault protein (MVP) induces type I IFN production during viral infection; however, little is known about the role of MVP in proinflammatory responses. In this study, we found in vitro that expression of MVP, IL-6, and IL-8 was inducible upon dsRNA stimulation or viral infection. Moreover, MVP was essential for the induction of IL-6 and IL-8, as impaired expression of IL-6 and IL-8 in MVP-deficient human PBMCs, human lung epithelial cells (A549), and THP-1 monocytes, as well as in murine splenocytes, peritoneal macrophages, and PBMCs from MVP-knockout (MVP(-/-)) mice, was observed...
March 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/26784167/comparative-proteomic-analysis-of-mature-and-immature-oocytes-of-the-swamp-buffalo-bubalus-bubalis
#8
Qiang Fu, Zhen-Fang Liu, Yu-Lin Huang, Yang-Qing Lu, Ming Zhang
Maternal protein components change markedly during mammalian oogenesis. Many of these proteins have yet to be characterized and verified. In this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the Swamp Buffalo (Bubalus bubalis). Proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. Image analysis indicated that 17 proteins were differentially expressed between the two groups...
January 14, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26754263/association-between-single-nucleotide-polymorphisms-in-dna-double-strand-break-repair-genes-and-prostate-cancer-aggressiveness-in-the-spanish-population
#9
L A Henríquez-Hernández, A Valenciano, P Foro-Arnalot, M J Álvarez-Cubero, J M Cozar, J F Suárez-Novo, M Castells-Esteve, P Fernández-Gonzalo, B De-Paula-Carranza, M Ferrer, F Guedea, G Sancho-Pardo, J Craven-Bartle, M J Ortiz-Gordillo, P Cabrera-Roldán, J I Rodríguez-Melcón, E Herrera-Ramos, C Rodríguez-Gallego, P C Lara
BACKGROUND: Novel predictors of prognosis and treatment response for prostate cancer (PCa) are required to better individualize treatment. Single-nucleotide polymorphisms (SNPs) in four genes directly (XRCC5 (X-ray repair complementing defective repair in Chinese hamster cells 5) and XRCC6 (X-ray repair complementing defective repair in Chinese hamster cells 6)) or indirectly (PARP1 and major vault protein (MVP)) involved in non-homologous end joining were examined in 494 Spanish PCa patients...
March 2016: Prostate Cancer and Prostatic Diseases
https://www.readbyqxmd.com/read/26499037/major-vault-protein-regulates-cell-growth-survival-signaling-through-oxidative-modifications
#10
Dividutta Das, Yi-Hsuan Wang, Cheng-Ying Hsieh, Yuichiro J Suzuki
Major vault protein forms a hollow, barrel-like structure in the cell called the vault, whose functions and regulation are not well understood. The present study reports that major vault protein regulates growth/survival signaling in human airway smooth muscle cells through oxidative modifications. The promotion of protein S-glutathionylation by asthma mediators such as interleukin-22 and platelet-derived growth factor or by knocking down glutaredoxin-1 or thioredoxin activated cell growth signaling. Mass spectrometry identified that major vault protein is glutathionylated...
January 2016: Cellular Signalling
https://www.readbyqxmd.com/read/26493711/vault-nanoparticles-packaged-with-enzymes-as-an-efficient-pollutant-biodegradation-technology
#11
Meng Wang, Danny Abad, Valerie A Kickhoefer, Leonard H Rome, Shaily Mahendra
Vault nanoparticles packaged with enzymes were synthesized as agents for efficiently degrading environmental contaminants. Enzymatic biodegradation is an attractive technology for in situ cleanup of contaminated environments because enzyme-catalyzed reactions are not constrained by nutrient requirements for microbial growth and often have higher biodegradation rates. However, the limited stability of extracellular enzymes remains a major challenge for practical applications. Encapsulation is a recognized method to enhance enzymatic stability, but it can increase substrate diffusion resistance, lower catalytic rates, and increase the apparent half-saturation constants...
November 24, 2015: ACS Nano
https://www.readbyqxmd.com/read/26476776/absence-of-association-between-major-vault-protein-mvp-gene-polymorphisms-and-drug-resistance-in-chinese-han-patients-with-partial-epilepsy
#12
Luo Zhou, Mengqi Zhang, Hongyu Long, Lili Long, Yuanyuan Xie, Zhaoqian Liu, Jin Kang, Qihua Chen, Li Feng, Bo Xiao
Drug resistance in epilepsy is common despite many antiepileptic drugs (AEDs) available for treatment. The development of drug resistant epilepsy may be a result of multiple factors. Several previous studies reported that the major vault protein (MVP) was significantly increased in epileptogenic brain tissues resected from patients with partial-onset seizures, indicating the possible involvement of MVP in drug resistance. In this article, we aimed to identify the association between single nucleotide polymorphisms (SNPs) of MVP gene and drug resistance of partial epilepsy in a Chinese Han population...
November 15, 2015: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/26456650/self-assembly-formed-by-a-short-dna-probe-pair-application-for-highly-sensitive-mrna-species-detection-without-reverse-transcription
#13
Mitsugu Usui, Toshihiko Fujikawa, Masako Osawa, Chikako Hakii, Natsumi Ikumi, Takamasa Nozaki, Noboru Kitamura, Yoshihiro Hatta, Shigeyoshi Fujiwara, Masami Takei
We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene β-actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs...
November 27, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26365998/dual-ph-and-temperature-responsive-protein-nanoparticles
#14
Nicholas M Matsumoto, George W Buchman, Leonard H Rome, Heather D Maynard
Multiply responsive protein nanoparticles are interesting for a variety of applications. Herein, we describe the synthesis of a vault nanoparticle that responds to both temperature and pH. Specifically, poly(N-isopropylacrylamide-co-acrylic acid) with a pyridyl disulfide end group was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymer had a lower critical solution temperature (LCST) of 31.9 °C at pH 5, 44.0 °C at pH 6 and above 60 °C at pH 7. The polymer was conjugated to human major vault protein (hMVP), and the resulting nanoparticle was analyzed by UV-Vis, dynamic light scattering (DLS) and electron microscopy...
August 1, 2015: European Polymer Journal
https://www.readbyqxmd.com/read/25952297/expression-of-the-vault-rna-protects-cells-from-undergoing-apoptosis
#15
Melanie Amort, Birgit Nachbauer, Selma Tuzlak, Arnd Kieser, Aloys Schepers, Andreas Villunger, Norbert Polacek
Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation...
May 8, 2015: Nature Communications
https://www.readbyqxmd.com/read/25869624/alteration-of-major-vault-protein-in-human-glioblastoma-and-its-relation-with-egfr-and-pten-status
#16
L Navarro, R Gil-Benso, J Megías, L Muñoz-Hidalgo, T San-Miguel, R C Callaghan, J M González-Darder, C López-Ginés, M J Cerdá-Nicolás
Glioblastoma (GBM) is the most frequent and malignant primary brain tumor. Conventional therapy of surgical removal, radiation and chemotherapy is largely palliative. Major vault protein (MVP), the main component of the vault organelle has been associated with multidrug resistance by reducing cellular accumulation of chemotherapeutic agents. With regard to cancer, MVP has been shown to be overexpressed in drug resistance development and malignant progression. The aim of the present study was to evaluate the MVP gene dosage levels in 113 archival samples from GBM and its correlation with patients' survival and epidermal growth factor receptor (EGFR) and phosphatase and tensin homolog (PTEN) gene dosages...
June 25, 2015: Neuroscience
https://www.readbyqxmd.com/read/25792599/a-retrovirus-packages-nascent-host-noncoding-rnas-from-a-novel-surveillance-pathway
#17
Matthew J Eckwahl, Soyeong Sim, Derek Smith, Alice Telesnitsky, Sandra L Wolin
Although all retroviruses recruit host cell RNAs into virions, both the spectrum of RNAs encapsidated and the mechanisms by which they are recruited remain largely unknown. Here, we used high-throughput sequencing to obtain a comprehensive description of the RNAs packaged by a model retrovirus, murine leukemia virus. The major encapsidated host RNAs are noncoding RNAs (ncRNAs) and members of the VL30 class of endogenous retroviruses. Remarkably, although Moloney leukemia virus (MLV) assembles in the cytoplasm, precursors to specific tRNAs, small nuclear RNAs (snRNAs), and small nucleolar RNAs (snoRNAs) are all enriched in virions...
March 15, 2015: Genes & Development
https://www.readbyqxmd.com/read/25706550/proteomic-analysis-of-pure-human-airway-gland-mucus-reveals-a-large-component-of-protective-proteins
#18
Nam Soo Joo, Idil Apak T Evans, Hyung-Ju Cho, Il-Ho Park, John F Engelhardt, Jeffrey J Wine
Airway submucosal glands contribute to innate immunity and protect the lungs by secreting mucus, which is required for mucociliary clearance and which also contains antimicrobial, anti-inflammatory, anti-proteolytic and anti-oxidant proteins. We stimulated glands in tracheal trimmings from three lung donors and collected droplets of uncontaminated mucus as they formed at the gland orifices under an oil layer. We analyzed the mucus using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analysis identified 5486 peptides and 441 proteins from across the 3 samples (269-319 proteins per subject)...
2015: PloS One
https://www.readbyqxmd.com/read/25603048/abcb1-abcc1-and-lrp-gene-expressions-are-altered-by-ldl-hdl-and-serum-deprivation-in-a-human-doxorubicin-resistant-uterine-sarcoma-cell-line
#19
Andréa Turbuck Celestino, Débora Levy, Jorge Luis Maria Ruiz, Sérgio Paulo Bydlowski
Multidrug resistance (MDR) is the major cause of cancer treatment failure. The ATP-binding cassette-B1 (ABCB1) transporter, also known as MDR1 or P-glycoprotein, is thought to promote the efflux of drugs from cells. MDR is also associated with the multidrug resistance-associated protein 1 (ABCC1) and the lung resistance-related protein (LRP), a human major vault protein. Moreover, MDR has a complex relationship with lipids. The ABCB1 has been reported to modulate cellular cholesterol homeostasis. Conversely, cholesterol has been reported to modulate multidrug transporters...
February 20, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25481566/human-hepatitis-b-virus-surface-and-e-antigens-inhibit-major-vault-protein-signaling-in-interferon-induction-pathways
#20
Shi Liu, Nanfang Peng, Jiajia Xie, Qian Hao, Mo Zhang, Yi Zhang, Zhangchuan Xia, Gang Xu, Fanpeng Zhao, Qing Wang, Tao Han, Ying Zhu
BACKGROUND & AIMS: We previously demonstrated that major vault protein (MVP) is a novel virus-induced host factor and its expression upregulates type-I interferon production, leading to cellular antiviral response. However, it remains unclear whether the antiviral function of MVP is impaired during hepatitis B virus (HBV) infection and what mechanisms are involved. Therefore, the aim of this study was to assess whether HBV can alter MVP expression despite the lack of type-I IFN induction and shed light on the underlying mechanisms HBV utilizes to evade host innate immune response...
May 2015: Journal of Hepatology
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