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major vault protein

Alyssa L Margiotta, Lisa J Bain, Charles D Rice
Cellular vaults are ubiquitous 13 mega Da multi-subunit ribonuceloprotein particles that may have a role in nucleocytoplasmic transport. Seventy percent of the vault's mass consists of a ≈100 kDa protein, the major vault protein. In humans, a drug resistance -associated protein, originally identified as lung resistance protein in metastatic lung cancer, was ultimately shown to be the previously described major vault protein. In this study, a partial major vault protein sequence was cloned from channel catfish...
July 15, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Karolina Wojtowicz, Radoslaw Januchowski, Michal Nowicki, Maciej Zabel
BACKGROUND/AIM: The definition of vault (ribonucleoprotein particles) function remains highly complex. Vaults may cooperate with multidrug resistance (MDR) proteins, supporting their role in drug resistance. This topic is the main theme of this publication. MATERIALS AND METHODS: The cell viability was determined by an MTT assay. The protein expression was detected by western blot analysis. The proteins were knocked-down using siRNA. RESULTS: No major vault protein (MVP) in the LoVo/Dx and W1PR cell lines after tunicamycin treatment was shown...
June 2017: Anticancer Research
Alba Cortés, Miguel García-Ferrús, Javier Sotillo, J Guillermo Esteban, Rafael Toledo, Carla Muñoz-Antolí
The current strategy for the control of helminth infections relies on chemotherapy. However, resistance appearance is promoting the necessity of developing new drugs against trematodes. Herein, potential trematocidal effects of garlic (Allium sativum) are investigated in the context of intestinal foodborne trematodes, employing the Echinostoma caproni-mouse model. Daily administration of dietary doses of garlic was conducted in three groups of mice: (i) before infection (prophylaxis), (ii) after infection (therapeutic) and (iii) both, before and after infection (continuous)...
August 2017: Parasitology Research
Aparna Banerjee Dixit, Devina Sharma, Arpna Srivastava, Jyotirmoy Banerjee, Manjari Tripathi, Deepak Prakash, P Sarat Chandra
PURPOSE: Identifying factors involved in the development of drug resistant epilepsy (DRE) remains a challenge. Candidate gene studies have shown modulation of resistance to drugs by various multidrug resistance proteins in DRE. However the resistance to drugs in DRE could be more complex and multifactorial involving molecules in different pharmacokinetic processes. In this study for the first time we have analyzed the relative expression of four molecules with different drug resistance mechanisms in two most common DRE pathologies, mesial temporal lobe epilepsy (MTLE) and focal cortical dysplasia (FCD) with respect to each other and also with different non-epileptic controls...
April 2017: Seizure: the Journal of the British Epilepsy Association
Mengmeng Xu, Long Che, Zhenguo Yang, Pan Zhang, Jiankai Shi, Jian Li, Yan Lin, Zhengfeng Fang, Lianqiang Che, Bin Feng, De Wu, Shengyu Xu
Primordial follicle formation represents a critical phase of the initiation of embryonic reproductive organ development, while the primordial follicle transition into primary follicle determines whether oestrus or ovulation will occur in female animals. To identify molecular mechanism of new proteins which are involved in ovarian development, we employed 2D-DIGE to compare the protein expression profiles of primordial follicles and primary follicles of fetal ovaries in pigs. Fetal ovaries were collected at distinct time-points of the gestation cycle (g55 and g90)...
2017: BioMed Research International
Yun Teng, Yi Ren, Xin Hu, Jingyao Mu, Abhilash Samykutty, Xiaoying Zhuang, Zhongbin Deng, Anil Kumar, Lifeng Zhang, Michael L Merchant, Jun Yan, Donald M Miller, Huang-Ge Zhang
Exosomes are emerging mediators of intercellular communication; whether the release of exosomes has an effect on the exosome donor cells in addition to the recipient cells has not been investigated to any extent. Here, we examine different exosomal miRNA expression profiles in primary mouse colon tumour, liver metastasis of colon cancer and naive colon tissues. In more advanced disease, higher levels of tumour suppressor miRNAs are encapsulated in the exosomes. miR-193a interacts with major vault protein (MVP)...
February 17, 2017: Nature Communications
Janina Jiang, Guangchao Liu, Valerie A Kickhoefer, Leonard H Rome, Lin-Xi Li, Stephen J McSorley, Kathleen A Kelly
Chlamydia trachomatis genital infection is the most common sexually transmitted bacterial disease, causing a significant burden to females due to reproductive dysfunction. Intensive screening and antibiotic treatment are unable to completely prevent female reproductive dysfunction, thus, efforts have become focused on developing a vaccine. A major impediment is identifying a safe and effective adjuvant which induces cluster of differentiation 4 (CD4) cells with attributes capable of halting genital infection and inflammation...
January 19, 2017: Vaccines
Yu-Lun Chen, Tsung-Ying Yang, Chieh-Liang Wu, Kun-Chieh Chen, Shih-Lan Hsu, Chi-Mei Hsueh
Lung resistance-related protein (LRP) is a human major vault protein (MVP) implicated in drug resistance of cancer cells in a cell-type dependent manner. The primary goal of the study was to understand the role(s) of LRP in doxorubicin (DOX) resistance of non-small cell lung cancer (NSCLC) cells and the underlying working mechanisms. In the study, the roles of LRP in the regulation of DOX dynamics, nuclear import of minor vault proteins (vault poly (ADP-ribose) polymerase, vPARP and telomerase associated protein-1, TEP-1) and DOX-mediated cytotoxicity were examined in CH27 and H460 cells...
December 31, 2016: Chinese Journal of Physiology
Nataliia V Shults, Dividutta Das, Yuichiro J Suzuki
Major vault protein (MVP) is the major component of the vault particle whose functions are not well understood. One proposed function of the vault is to serve as a mechanism of drug transport, which confers drug resistance in cancer cells. We show that MVP can be found in cardiac and smooth muscle. In human airway smooth muscle cells, knocking down MVP was found to cause cell death, suggesting that MVP serves as a cell survival factor. Further, our laboratory found that MVP is S-glutathionylated in response to ligand/receptor-mediated cell signaling...
2016: Receptors & Clinical Investigation
Ting-Ting Song, Dan Li, Shao-Ping Huang, Lin Yang, Xue-Ying Wang, Yong-Sheng Jiang, Yu Liu
OBJECTIVE: To study the effect of cyclooxygenase -2 selective inhibitor celecoxib on the expression of major vault protein ( MVP) in the brain of rats with status epilepticus and its possible roles in the treatment of refractory epilepsy. METHODS: Sixty adult male Sprague-Dawley rats were randomly assigned to blank control (n=16), epilepsy model (n=22) and celecoxib treatment groups (n=22). After the status epilepticus was induced in rats by injecting lithium and pilocarpine, each group had 16 rats enrolled as subjects...
May 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Ya-Nan Zhao, Dong-Ning He, Ya-DI Wang, Jun-Jie Li, Min-Wen Ha
The human major vault protein (MVP) has been linked to the development of multidrug resistance in cancer cells, and overexpression of MVP has been observed in ovarian cancer tissues. The aim of the present study was to investigate the association between single nucleotide polymorphisms (SNPs) in the MVP gene and the tumor response to platinum-based chemotherapy and survival of patients affected by epithelial ovarian cancer (EOC), in addition to confirm whether tetra-primer amplification-refractory mutation system (ARMS)-polymerase chain reaction (PCR) is an accurate genotyping method...
April 2016: Oncology Letters
Dario Barbone, Loes Van Dam, Carlo Follo, Puthen V Jithesh, Shu-Dong Zhang, William G Richards, Raphael Bueno, Dean A Fennell, V Courtney Broaddus
To investigate the underlying causes of chemoresistance in malignant pleural mesothelioma, we have studied mesothelioma cell lines as 3D spheroids, which acquire increased chemoresistance compared to 2D monolayers. We asked whether the gene expression of 3D spheroids would reveal mechanisms of resistance. To address this, we measured gene expression of three mesothelioma cell lines, M28, REN and VAMT, grown as 2D monolayers and 3D spheroids. A total of 209 genes were differentially expressed in common by the three cell lines in 3D (138 upregulated and 71 downregulated), although a clear resistance pathway was not apparent...
2016: PloS One
Xinhong Wang, Yasmine F Ibrahim, Dividutta Das, Makhosazane Zungu-Edmondson, Nataliia V Shults, Yuichiro J Suzuki
AIMS: Pulmonary arterial hypertension (PAH) remains a lethal disease with pronounced narrowing of pulmonary vessels due to abnormal cell growth. Agents that can reduce the pulmonary vascular thickness thus have therapeutic potential. The present study investigated the efficacy of carfilzomib (CFZ), a proteasome inhibitor and a cancer chemotherapeutic drug, on reversing PAH. METHODS AND RESULTS: In two rat models of PAH, SU5416/hypoxia and SU5416/ovalbumin, CFZ effectively reversed pulmonary vascular remodelling with the promotion of apoptosis and autophagy...
May 15, 2016: Cardiovascular Research
Nanfang Peng, Shi Liu, Zhangchuan Xia, Sheng Ren, Jian Feng, Mingzhen Jing, Xin Gao, Erik A C Wiemer, Ying Zhu
Pathogen invasion triggers robust antiviral cytokine production via different transcription factor signaling pathways. We have previously demonstrated that major vault protein (MVP) induces type I IFN production during viral infection; however, little is known about the role of MVP in proinflammatory responses. In this study, we found in vitro that expression of MVP, IL-6, and IL-8 was inducible upon dsRNA stimulation or viral infection. Moreover, MVP was essential for the induction of IL-6 and IL-8, as impaired expression of IL-6 and IL-8 in MVP-deficient human PBMCs, human lung epithelial cells (A549), and THP-1 monocytes, as well as in murine splenocytes, peritoneal macrophages, and PBMCs from MVP-knockout (MVP(-/-)) mice, was observed...
March 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Qiang Fu, Zhen-Fang Liu, Yu-Lin Huang, Yang-Qing Lu, Ming Zhang
Maternal protein components change markedly during mammalian oogenesis. Many of these proteins have yet to be characterized and verified. In this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the Swamp Buffalo (Bubalus bubalis). Proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. Image analysis indicated that 17 proteins were differentially expressed between the two groups...
January 14, 2016: International Journal of Molecular Sciences
L A Henríquez-Hernández, A Valenciano, P Foro-Arnalot, M J Álvarez-Cubero, J M Cozar, J F Suárez-Novo, M Castells-Esteve, P Fernández-Gonzalo, B De-Paula-Carranza, M Ferrer, F Guedea, G Sancho-Pardo, J Craven-Bartle, M J Ortiz-Gordillo, P Cabrera-Roldán, J I Rodríguez-Melcón, E Herrera-Ramos, C Rodríguez-Gallego, P C Lara
BACKGROUND: Novel predictors of prognosis and treatment response for prostate cancer (PCa) are required to better individualize treatment. Single-nucleotide polymorphisms (SNPs) in four genes directly (XRCC5 (X-ray repair complementing defective repair in Chinese hamster cells 5) and XRCC6 (X-ray repair complementing defective repair in Chinese hamster cells 6)) or indirectly (PARP1 and major vault protein (MVP)) involved in non-homologous end joining were examined in 494 Spanish PCa patients...
March 2016: Prostate Cancer and Prostatic Diseases
Dividutta Das, Yi-Hsuan Wang, Cheng-Ying Hsieh, Yuichiro J Suzuki
Major vault protein forms a hollow, barrel-like structure in the cell called the vault, whose functions and regulation are not well understood. The present study reports that major vault protein regulates growth/survival signaling in human airway smooth muscle cells through oxidative modifications. The promotion of protein S-glutathionylation by asthma mediators such as interleukin-22 and platelet-derived growth factor or by knocking down glutaredoxin-1 or thioredoxin activated cell growth signaling. Mass spectrometry identified that major vault protein is glutathionylated...
January 2016: Cellular Signalling
Meng Wang, Danny Abad, Valerie A Kickhoefer, Leonard H Rome, Shaily Mahendra
Vault nanoparticles packaged with enzymes were synthesized as agents for efficiently degrading environmental contaminants. Enzymatic biodegradation is an attractive technology for in situ cleanup of contaminated environments because enzyme-catalyzed reactions are not constrained by nutrient requirements for microbial growth and often have higher biodegradation rates. However, the limited stability of extracellular enzymes remains a major challenge for practical applications. Encapsulation is a recognized method to enhance enzymatic stability, but it can increase substrate diffusion resistance, lower catalytic rates, and increase the apparent half-saturation constants...
November 24, 2015: ACS Nano
Luo Zhou, Mengqi Zhang, Hongyu Long, Lili Long, Yuanyuan Xie, Zhaoqian Liu, Jin Kang, Qihua Chen, Li Feng, Bo Xiao
Drug resistance in epilepsy is common despite many antiepileptic drugs (AEDs) available for treatment. The development of drug resistant epilepsy may be a result of multiple factors. Several previous studies reported that the major vault protein (MVP) was significantly increased in epileptogenic brain tissues resected from patients with partial-onset seizures, indicating the possible involvement of MVP in drug resistance. In this article, we aimed to identify the association between single nucleotide polymorphisms (SNPs) of MVP gene and drug resistance of partial epilepsy in a Chinese Han population...
November 15, 2015: Journal of the Neurological Sciences
Mitsugu Usui, Toshihiko Fujikawa, Masako Osawa, Chikako Hakii, Natsumi Ikumi, Takamasa Nozaki, Noboru Kitamura, Yoshihiro Hatta, Shigeyoshi Fujiwara, Masami Takei
We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene β-actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs...
November 27, 2015: Biochemical and Biophysical Research Communications
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