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tracheal regeneration

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https://www.readbyqxmd.com/read/28941146/tracheal-repair-with-acellular-human-amniotic-membrane-in-a-rabbit-model
#1
Lianna Ferrari Jorge, Julio C Francisco, Nelson Bergonse, Cristina Baena, Katherine Athayde T Carvalho, Eltyeb Abdelwahid, Jose Rocha Faria Neto, Luiz Felipe Pinho Moreira, Luiz Cesar Guarita-Souza
BACKGROUND: Surgical correction of tracheal stenosis is still a complex and challenging procedure. Acellular human amniotic membranes (AHAM) represent a promising biomaterial source for tissue regeneration. The aim of this study was to evaluate whether AHAM grafts improve tissue regeneration of the trachea in a rabbit model of tracheostomy. METHODS: Twenty rabbits were randomized into two groups. Animals in the control group underwent surgical tracheostomy only and animals in the AHAM group underwent surgical tracheostomy and received an AHAM graft that covered the defect site...
September 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28934345/mesenchymal-stem-cell-therapy-for-laryngotracheal-stenosis-a-systematic-review-of-preclinical-studies
#2
REVIEW
Kathrine Kronberg Jakobsen, Christian Grønhøj, David H Jensen, Anne Fischer-Nielsen, Thomas Hjuler, Christian von Buchwald
BACKGROUND: Laryngotracheal stenosis (LTS) can be either congenital or acquired. Laryngeal stenosis is most often encountered after prolonged intubation. The mechanism for stenosis following intubation is believed to be hypertrophic scarring. Mesenchymal stem cells (MSCs) therapy has shown promising results in regenerative medicine. We aimed to systematically review the literature on MSC therapy for stenosis of the conductive airways. METHODS: PubMed, EMBASE, Google Scholar and the Cochrane Library were systematically searched from January 1980-January 2017 with the purpose of identifying all studies addressing the effect of MSC therapy on the airway...
2017: PloS One
https://www.readbyqxmd.com/read/28819688/airway-reconstruction-using-decellularized-tracheal-allografts-in-a-porcine-model
#3
Michinobu Ohno, Yasushi Fuchimoto, Huai-Che Hsu, Masataka Higuchi, Makoto Komura, Tetsuji Yamaoka, Akihiro Umezawa, Shin Enosawa, Tatsuo Kuroda
PURPOSE: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. METHODS: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies...
August 17, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28702984/regenerative-medicine-in-the-treatment-of-gastro-esophageal-reflux-disease-and-laryngo-pharyngeal-reflux-from-research-to-cure
#4
S E Aragona, G Mereghetti, M Bianchetti, B Mangiavillano, T Zurlo, J Lotti, I La Mantia, K França, T Lotti
We present our observational study on 40 patients treated with the medical device containing sodium hyaluronic acid and magnesium alginate, performing a lubricating and hydrating action. This device is in the form of a gel, with topical action to contrast gastroesophageal reflux and to exert a mechanical role of protection of the mucosal tissues (mouth-pharyngo-esophageal mucosa and gastric mucose.). Forty patients were recruited aged between 22- and 72-years-old with painful dyspeptic Gastro Esophageal Reflux Disease (GERD) symptomatology in acute or in clinical phase (25 patients) and with pharyngolaryngo- tracheal symptomatology (15 patients)...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28658997/evaluation-of-the-potential-of-kartogenin-encapsulated-poly-l-lactic-acid-co-caprolactone-collagen-nanofibers-for-tracheal-cartilage-regeneration
#5
Haiyue Yin, Juan Wang, Ziqi Gu, Wenhao Feng, Manchen Gao, Yu Wu, Hao Zheng, Xiaomin He, Xiumei Mo
Tracheal stenosis is one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, we fabricated coaxial electrospun fibers using poly(L-lactic acid-co-caprolactone) and collagen solution as shell fluid and kartogenin solution as core fluid. Scanning electron microscope and transmission electron microscope images demonstrated that nanofibers had uniform and smooth structure...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28604083/implantation-of-induced-pluripotent-stem-cell-derived-tracheal-epithelial-cells
#6
Masakazu Ikeda, Mitsuyoshi Imaizumi, Susumu Yoshie, Ryosuke Nakamura, Koshi Otsuki, Shigeyuki Murono, Koichi Omori
OBJECTIVES: Compared with using autologous tissue, the use of artificial materials in the regeneration of tracheal defects is minimally invasive. However, this technique requires early epithelialization on the inner side of the artificial trachea. After differentiation from induced pluripotent stem cells (iPSCs), tracheal epithelial tissues may be used to produce artificial tracheas. Herein, we aimed to demonstrate that after differentiation from fluorescent protein-labeled iPSCs, tracheal epithelial tissues survived in nude rats with tracheal defects...
July 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28546133/tissue-engineered-trachea-regeneration-using-decellularized-trachea-matrix-treated-with-laser-micropore-technique
#7
Yong Xu, Dan Li, Zongqi Yin, Aijuan He, Miaomiao Lin, Gening Jiang, Xiao Song, Xuefei Hu, Yi Liu, Jinpeng Wang, Xiaoyun Wang, Liang Duan, Guangdong Zhou
Tissue-engineered trachea provides a promising approach for reconstruction of long segmental tracheal defects. However, a lack of ideal biodegradable scaffolds greatly restricts its clinical translation. Decellularized trachea matrix (DTM) is considered a proper scaffold for trachea cartilage regeneration owing to natural tubular structure, cartilage matrix components, and biodegradability. However, cell residual and low porosity of DTM easily result in immunogenicity and incomplete cartilage regeneration. To address these problems, a laser micropore technique (LMT) was applied in the current study to modify trachea sample porosity to facilitate decellular treatment and cell ingrowth...
May 22, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28359676/transplantation-of-the-decellularized-tracheal-allograft-in-animal-model-rabbit
#8
Reza Ershadi, Mohamadbagher Rahim, Siavash Jahany, Siamak Rakei
BACKGROUND: It has been difficult to perform tracheal allotransplantation without immunosuppression. To determine whether decellularized trachea can be used in tracheal replacement, we evaluated the viability of decellularized tracheal allografts in a rabbit model of immunosuppressant-free transplantation. METHOD: Half allograft (Group 1, n = 7) was harvested from adult New Zealand white rabbits, subjected to a detergent-enzymatic method (containing sodium deoxycholate/DNase lavations) of decellularization for as many cycles as needed, and the other half was stored in phosphate-buffered saline at 4°C as a control (Group 2, n = 7)...
March 27, 2017: Asian Journal of Surgery
https://www.readbyqxmd.com/read/28131109/fabrication-of-chitosan-silk-based-tracheal-scaffold-using-freeze-casting-method
#9
Zeinab Nematollahi, Mohammad Tafazzoli-Shadpour, Ali Zamanian, Amir Seyedsalehi, Shadmehr Mohammad-Behgam, Fariba Ghorbani, Fereshte Mirahmadi
Background: Since the treatments of long tracheal lesions are associated with some limitations, tissue engineered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods: Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) and glutaraldehyde (GA) concentrations (0, 0.4, and 0.8 wt%). Samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments...
July 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28109571/in%C3%A2-vivo-tissue-engineering-of-human%C3%A2-airways
#10
Emmanuel Martinod, Joseph Paquet, Hervé Dutau, Dana M Radu, Morad Bensidhoum, Sébastien Abad, Yurdagül Uzunhan, Eric Vicaut, Hervé Petite
BACKGROUND: Airway transplantation remains a major challenge in thoracic surgery. Based on our previous laboratory work, we developed the techniques required to bioengineer a tracheal substitute in vivo using cryopreserved aortic allografts as biological matrices (Replacement of the Airways and/or the Pulmonary Vessels Using a Cryopreserved Arterial Allograft [TRACHEOBRONCART] Study, NCT01331863). We present here 2 patients who had a definitive tracheostomy for complex laryngotracheal stenoses refractory to conventional therapy...
May 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28105760/aerosol-based-airway-epithelial-cell-delivery-improves-airway-regeneration-and-repair
#11
E Kardia, E S Ch'ng, B H Yahaya
Aerosol-based cell therapy has emerged as a novel and promising therapeutic strategy for treating lung diseases. The goal of this study was to determine the safety and efficacy of aerosol-based airway epithelial cell (AEC) delivery in the setting of acute lung injury induced by tracheal brushing in rabbit. Twenty-four hours following injury, exogenous rabbit AECs were labelled with bromodeoxyuridine and aerosolized using the MicroSprayer® Aerosolizer into the injured airway. Histopathological assessments of the injury in the trachea and lungs were quantitatively scored (1 and 5 days after cell delivery)...
January 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28101973/heparin-cross-linked-collagen-sponge-scaffolds-improve-functional-regeneration-of-rat-tracheal-epithelium
#12
Ryosuke Nakamura, Akiko Tani, Susumu Yoshie, Masakazu Ikeda, Ikuo Wada, Akihiro Hazama, Yukio Nomoto, Yasuhiro Tada, Tatsuo Nakamura, Koichi Omori
Tracheal epithelial cells maintain airway homeostasis by mediating mucociliary clearance. Following tracheal reconstruction, timely epithelial regeneration is required to prevent respiratory compromise and infectious diseases. To achieve rapid tracheal epithelial regeneration, a heparin cross-linked collagen sponge containing fibroblast growth factor-2 (FGF-2) was prepared as a graft for tracheal reconstruction. The heparin cross-linked sponge exhibited a high FGF-2 retaining capacity, and tracheal epithelial and mesenchymal cells cultured in this sponge containing FGF-2 showed high proliferative capacities...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27935870/changes-in-the-methylation-status-of-the-oct3-4-nanog-and-sox2-promoters-in-stem-cells-during-regeneration-of-rat-tracheal-epithelium-after-injury
#13
Ying Zhou, Nan Song, Xin Li, Ying Han, Zihan Ren, Jing-Xian Xu, Yu-Chen Han, Fang Li, Xinshan Jia
We investigated the relationship between promoter methylation and tracheal stem cell activation. We developed a model of rat tracheal epithelium regeneration after 5-fluorouracil (5-FU)-induced injury. Using immunohistochemistry and Western blotting, the expression levels of the stem cell pluripotency regulator Oct3/4 and differentiation marker CK14 were measured after 5-FU treatment. The methylation status of the Oct3/4, Nanog, and Sox2 promoters was investigated using methylation-specific PCR. Additionally, the effects of 5-azacytidine (5-azaC), a demethylating agent, on Oct3/4, Nanog, and Sox2 mRNA and protein expression were evaluated...
January 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/27925229/plga-ptmc-cultured-bone-mesenchymal-stem-cell-scaffold-enhances-cartilage-regeneration-in-tissue-engineered-tracheal-transplantation
#14
Bingyang Yan, Zhipei Zhang, Xiaoping Wang, Yunfeng Ni, Yongshi Liu, Tao Liu, Wuping Wang, Hao Xing, Ying Sun, Jian Wang, Xiao-Fei Li
The treatment of long-segment tracheal defect requires the transplantation of effective tracheal substitute, and the tissue-engineered trachea (TET) has been proposed as an ideal tracheal substitute. The major cause of the failure of segmental tracheal defect reconstruction by TET is airway collapse caused by the chondromalacia of TET cartilage. The key to maintain the TET structure is the regeneration of chondrocytes in cartilage, which can secrete plenty of cartilage matrices. To address the problem of the chondromalacia of TET cartilage, this study proposed an improved strategy...
May 2017: Artificial Organs
https://www.readbyqxmd.com/read/27885873/effects-of-artificial-tracheal-fixation-on-tracheal-epithelial-regeneration-and-prevention-of-tracheal-stenosis
#15
Yuta Nakaegawa, Ryosuke Nakamura, Yasuhiro Tada, Ryo Suzuki, Toshiaki Takezawa, Tatsuo Nakamura, Koichi Omori
CONCLUSION: Tight fixation of the artificial trachea is important for epithelialization and tracheal stenosis. OBJECTIVE: The authors have developed an artificial trachea and have used it for tracheal reconstruction. Although various studies on tracheal reconstruction have been conducted, no studies have examined the effect of artificial tracheal fixation on tracheal stenosis and regeneration. Therefore, the purpose of the present study was to evaluate the effect of artificial tracheal fixation...
June 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27873529/tracheal-allotransplantation-and-regeneration
#16
REVIEW
P Delaere, M Molitor
Non-malignant and malignant obstruction of the tracheal airway causes significant morbidity and mortality. With increased use of artificial airways, benign and iatrogenic complications are increasing. A tracheal stenosis that is less than 5 cm in length can be resected with end-to-end anastomosis. Longer tracheal lesions can be treated in a palliative way by placement of a stent to secure airway lumen patency. The management of tracheal defects is an evolving field. Tracheal transplantation and tracheal regeneration may provide major treatment advances to cases with long-segment tracheal involvement...
December 0: Acta Chirurgiae Plasticae
https://www.readbyqxmd.com/read/27770937/evaluation-of-the-potential-of-rhtgf-%C3%AE-3-encapsulated-p-lla-cl-collagen-nanofibers-for-tracheal-cartilage-regeneration-using-mesenchymal-stems-cells-derived-from-wharton-s-jelly-of-human-umbilical-cord
#17
Jing Wang, Binbin Sun, Lingling Tian, Xiaomin He, Qiang Gao, Tong Wu, Seeram Ramakrishna, Jinghao Zheng, Xiumei Mo
Tracheal injuries are one of major challenging issues in clinical medicine because of the poor intrinsic ability of tracheal cartilage for repair. Tissue engineering provides an alternative method for the treatment of tracheal defects by generating replacement tracheal structures. In this study, core-shell nanofibrous scaffold was fabricated to encapsulate bovine serum albumin & rhTGF-β3 (recombinant human transforming growth factor-β3) into the core of the nanofibers for tracheal cartilage regeneration. Characterization of the core-shell nanofibrous scaffold was carried out by scanning electron microscope (SEM), transmission electron microscope (TEM), laser scanning confocal microscopy (LSCM), and tensile mechanical test...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27758155/an-investigation-of-topics-and-trends-of-tracheal-replacement-studies-using-co-occurrence-analysis
#18
REVIEW
Fariba Ghorbani, Mansoureh Feizabadi, Roya Farzanegan, Esmaeil Vaziri, Saeed Samani, Seyedamirmohammad Lajevardi, Lida Moradi, Mohammad Behgam Shadmehr
This study evaluated tracheal regeneration studies using scientometric and co-occurrence analysis to identify the most important topics and assess their trends over time. To provide the adequate search options, PubMed, Scopus, and Web of Science (WOS) were used to cover various categories such as keywords, countries, organizations, and authors. Search results were obtained by employing Bibexcel. Co-occurrence analysis was applied to evaluate the publications. Finally, scientific maps, author's network, and country contributions were depicted using VOSviewer and NetDraw...
April 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27696973/tracheal-tissue-engineering-in-vivo-biocompatibility-of-mechanically-stripped-allogenic-rabbit-trachea-with-autologous-epithelial-covering
#19
Margot Den Hondt, Bart M Vanaudenaerde, Erik K Verbeken, Jan J Vranckx
BACKGROUND: Successful trachea transplantation comprises the use of biocompatible constructs with little immune-reactivity, submucosal revascularization and creation of an epithelial covering. Allogenic chondrocytes might be protected from an overt immune-response due to physical isolation. Our aim was to evaluate in-vivo biocompatibility of allotracheae, stripped of their highly-immunogenic inner lining. Secondly, we established whether these constructs might serve as suitable scaffolds for autologous epithelial grafting...
October 4, 2016: Acta Chirurgica Belgica
https://www.readbyqxmd.com/read/27520928/engineered-tissue-stent-biocomposites-as-tracheal-replacements
#20
Liping Zhao, Sumati Sundaram, Andrew V Le, Angela H Huang, Jiasheng Zhang, Go Hatachi, Arkadi Beloiartsev, Michael G Caty, Tai Yi, Katherine Leiby, Ashley Gard, Mehmet H Kural, Liqiong Gui, Kevin A Rocco, Amogh Sivarapatna, Elizabeth Calle, Allison Greaney, Luca Urbani, Panagiotis Maghsoudlou, Alan Burns, Paolo DeCoppi, Laura E Niklason
Here we report the creation of a novel tracheal construct in the form of an engineered, acellular tissue-stent biocomposite trachea (TSBT). Allogeneic or xenogeneic smooth muscle cells are cultured on polyglycolic acid polymer-metal stent scaffold leading to the formation of a tissue comprising cells, their deposited collagenous matrix, and the stent material. Thorough decellularization then produces a final acellular tubular construct. Engineered TSBTs were tested as end-to-end tracheal replacements in 11 rats and 3 nonhuman primates...
September 2016: Tissue Engineering. Part A
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