keyword
MENU ▼
Read by QxMD icon Read
search

COLON MSI

keyword
https://www.readbyqxmd.com/read/29284010/safety-and-antitumor-activity-of-the-anti-pd-1-antibody-pembrolizumab-in-patients-with-advanced-colorectal-carcinoma
#1
Bert H O'Neil, John M Wallmark, David Lorente, Elena Elez, Judith Raimbourg, Carlos Gomez-Roca, Samuel Ejadi, Sarina A Piha-Paul, Mark N Stein, Albiruni R Abdul Razak, Katia Dotti, Armando Santoro, Roger B Cohen, Marlena Gould, Sanatan Saraf, Karen Stein, Sae-Won Han
BACKGROUND: Colorectal cancers (CRCs) expressing programmed death ligand 1 (PD-L1) have poor prognosis. In the multicohort KEYNOTE-028 trial, the anti-PD-1 antibody pembrolizumab was evaluated in 20 PD-L1-positive advanced solid tumors. Herein, we report results for the advanced CRC cohort. METHODS: Patients with advanced, treatment-resistant PD-L1-positive carcinoma of the colon or rectum were enrolled, regardless of microsatellite instability (MSI) status. Pembrolizumab 10 mg/kg was administered every 2 weeks for up to 2 years or until disease progression/unacceptable toxicity...
2017: PloS One
https://www.readbyqxmd.com/read/29230882/molecular-profiling-and-genome-wide-analysis-based-on-somatic-copy-number-alterations-in-advanced-colorectal-cancers
#2
Tamotsu Sugai, Yayoi Takahashi, Makoto Eizuka, Ryo Sugimoto, Yasuko Fujita, Wataru Habano, Kouki Otsuka, Akira Sasaki, Eiichiro Yamamoto, Takayuki Matsumoto, Hiromu Suzuki
To characterize somatic alterations in colorectal cancer (CRC), we conducted a genome-scale analysis of 106 CRC specimens. We assessed comprehensive somatic copy number alterations (SCNAs) in these CRC specimens. In addition, we examined microsatellite instability (MSI; low and high), genetic mutations (KRAS, BRAF, TP53, and PIK3CA), and DNA methylation status (classified into low, intermediate, and high type). We stratified molecular alterations in the CRCs using a hierarchical cluster analysis. The examined CRCs could be categorized into 3 subgroups using hierarchical cluster analysis...
December 12, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29227110/itraq-quantitative-proteomic-profiling-and-maldi-msi-of-colon-cancer-spheroids-treated-with-combination-chemotherapies-in-a-3d-printed-fluidic-device
#3
Gabriel J LaBonia, Katelyn R Ludwig, C Bruce Mousseau, Amanda B Hummon
For a patient with metastatic colorectal cancer there are limited clinical options aside from chemotherapy. Unfortunately, the development of new chemotherapeutics is a long and costly process. New methods are needed to identify promising drug candidates earlier in the drug development process. Most chemotherapies are administered to patients in combinations. Here, an in vitro platform is used to assess the penetration and metabolism of combination chemotherapies in three-dimensional colon cancer cell cultures, or spheroids...
December 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29227006/major-improvement-in-the-detection-of-microsatellite-instability-in-colorectal-cancer-using-hsp110-t17-e-ice-cold-pcr
#4
Alexandre How-Kit, Antoine Daunay, Olivier Buhard, Clément Meiller, Mourad Sahbatou, Ada Collura, Alex Duval, Jean-François Deleuze
Every colorectal cancer (CRC) patient should be tested for microsatellite instability (MSI) to screen for Lynch syndrome. Evaluation of MSI status involves screening tumor DNA for the presence of somatic deletions in DNA repeats using PCR followed by fragment analysis. While this method may lack sensitivity due to the presence of a high level of germline DNA, which frequently contaminates the core of primary colon tumors, no other method developed to date is capable of modifying the standard PCR protocol to achieve improvement of MSI detection...
December 11, 2017: Human Mutation
https://www.readbyqxmd.com/read/29222172/proximal-aberrant-crypt-foci-associate-with-synchronous-neoplasia-and-are-primed-for-neoplastic-progression
#5
David A Drew, Allen Mo, James J Grady, Richard G Stevens, Joel B Levine, Bruce M Brenner, Joseph C Anderson, Faripour Forouhar, Michael J O'Brien, Thomas J Devers, Daniel W Rosenberg
Aberrant crypt foci (ACF) are the earliest morphologically identifiable lesion found within the human colon. Despite their relatively high frequency in the distal colon, few studies have examined the molecular characteristics of ACF within the proximal colon. In the following study, clinical participants (n=184) were screened for ACF using high-definition chromoendoscopy with contrast dye-spray. Following pathological confirmation, ACF biopsies were subjected to laser-capture microdissection (LCM) and epithelial cells were evaluated for somatic mutations with a customized colorectal cancer mutation panel using DNA-mass spectrometry...
December 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29213343/exome-sequencing-characterizes-the-somatic-mutation-spectrum-of-early-serrated-lesions-in-a-patient-with-serrated-polyposis-syndrome-sps
#6
Sukanya Horpaopan, Jutta Kirfel, Sophia Peters, Michael Kloth, Robert Hüneburg, Janine Altmüller, Dmitriy Drichel, Margarete Odenthal, Glen Kristiansen, Christian Strassburg, Jacob Nattermann, Per Hoffmann, Peter Nürnberg, Reinhard Büttner, Holger Thiele, Philip Kahl, Isabel Spier, Stefan Aretz
Background: Serrated or Hyperplastic Polyposis Syndrome (SPS, HPS) is a yet poorly defined colorectal cancer (CRC) predisposition characterised by the occurrence of multiple and/or large serrated polyps throughout the colon. A serrated polyp-CRC sequence (serrated pathway) of CRC formation has been postulated, however, to date only few molecular signatures of serrated neoplasia (BRAF, KRAS, RNF43 mutations, CpG Island Methylation, MSI) have been described in a subset of SPS patients and neither the etiology of the syndrome nor the distinct genetic alterations during tumorigenesis have been identified...
2017: Hereditary Cancer in Clinical Practice
https://www.readbyqxmd.com/read/29212164/targeted-sequencing-of-established-and-candidate-colorectal-cancer-genes-in-the-colon-cancer-family-registry-cohort
#7
Leon Raskin, Yan Guo, Liping Du, Mark Clendenning, Christophe Rosty, Noralane M Lindor, Stephen B Gruber, Daniel D Buchanan
The underlying genetic cause of colorectal cancer (CRC) can be identified for 5-10% of all cases, while at least 20% of CRC cases are thought to be due to inherited genetic factors. Screening for highly penetrant mutations in genes associated with Mendelian cancer syndromes using next-generation sequencing (NGS) can be prohibitively expensive for studies requiring large samples sizes. The aim of the study was to identify rare single nucleotide variants and small indels in 40 established or candidate CRC susceptibility genes in 1,046 familial CRC cases (including both MSS and MSI-H tumor subtypes) and 1,006 unrelated controls from the Colon Cancer Family Registry Cohort using a robust and cost-effective DNA pooling NGS strategy...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29187431/microsatellite-instability-as-a-prognostic-factor-in-stage-ii-colon-cancer-patients-a-meta-analysis-of-published-literature
#8
REVIEW
Ioannis Gkekas, Jan Novotny, Ladislav Pecen, Karin Strigård, Richard Palmqvist, Ulf Gunnarsson
BACKGROUND/AIM: The prognostic role of microsatellite instability (MSI) in stage II colon cancer patients remains controversial despite the fact that it has been investigated in a number of studies. Hazard ratios differ considerably among these studies. We performed a meta-analysis to define the significance of MSI in this group of patients. MATERIALS AND METHODS: Studies indexed in PubMed presenting separate data on MSI status and survival outcomes for stage II colon cancer patients have been analyzed using fixed-effect meta-analysis of hazard ratio (HR) according to the method of Peto...
December 2017: Anticancer Research
https://www.readbyqxmd.com/read/29180604/classifying-colorectal-cancer-by-tumor-location-rather-than-sidedness-highlights-a-continuum-in-mutation-profiles-and-consensus-molecular-subtypes
#9
Jonathan M Loree, Allan Al Pereira, Michael Lam, Alexandra N Willauer, Kanwal Raghav, Arvind Dasari, Van K Morris, Shailesh M Advani, David G Menter, Cathy Eng, Kenna Shaw, Russell Broaddus, Mark J Routbort, Yusha Liu, Jeffrey Morris, Rajyalakshmi Luthra, Funda Meric-Bernstam, Michael J Overman, Dipen M Maru, Scott Kopetz
PURPOSE: Colorectal cancers (CRCs) are classified as right/left sided based on whether they occur before/after the splenic flexure, with established differences in molecular subtypes and outcomes.  However, it is unclear if this division is optimal and whether precise tumor location provides further information. EXPERIMENTAL DESIGN: In 1,876 patients with CRC we compared mutation prevalence and overall survival (OS) according to side and location. Consensus Molecular Subtype (CMS) was compared in a separate cohort of 608 patients...
November 27, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29167892/association-of-prognostic-value-of-primary-tumor-location-in-stage-iii-colon-cancer-with-ras-and-braf-mutational-status
#10
Julien Taieb, Hampig Raphael Kourie, Jean-François Emile, Karine Le Malicot, Ralyath Balogoun, Josep Tabernero, Enrico Mini, Gunnar Folprecht, Jean-Luc Van Laethem, Claire Mulot, Olivier Bouché, Thomas Aparicio, Pierre Michel, Josef Thaler, John Bridgewater, Eric Van Cutsem, Géraldine Perkins, Come Lepage, Ramon Salazar, Pierre Laurent-Puig
Importance: We know of no data on the prognostic value of primary tumor location (PTL) according to BRAF, RAS, and microsatellite instability (MSI) status in patients who have undergone resection for colon cancer (CC) and have been treated with current standard adjuvant chemotherapy. Objective: To determine the prognostic and predictive value of PTL according to BRAF, RAS, and MSI status in patients with stage III CC receiving adjuvant treatment with FOLFOX (folinic acid [leucovorin calcium], fluorouracil, and oxaliplatin) with or without cetuximab...
November 22, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/29137623/analysis-of-factors-influencing-molecular-testing-at-diagnostic-of-colorectal-cancer
#11
Quentin Thiebault, Gautier Defossez, Lucie Karayan-Tapon, Pierre Ingrand, Christine Silvain, David Tougeron
BACKGROUND: The aim of the study was to evaluate the current rate of molecular testing prescription (KRAS codons 12/13, BRAF and microsatellite instability (MSI)) in newly diagnosed colorectal cancer (CRC) patients and to determine which factors influence testing. METHODS: All incident CRC cases in 2010 were identified in the Poitou-Charentes General Cancer Registry. The exhaustive molecular testing performed was accessed in the French molecular genetics platform...
November 14, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29137387/infrequently-expressed-mirnas-influence-survival-after-diagnosis-with-colorectal-cancer
#12
Martha L Slattery, Andrew J Pellatt, Frances Y Lee, Jennifer S Herrick, Wade S Samowitz, John R Stevens, Roger K Wolff, Lila E Mullany
Half of miRNAs expressed in colorectal tissue are expressed < 50% of the population. Many infrequently expressed miRNAs have low levels of expression. We hypothesize that less frequently expressed miRNAs, when expressed at higher levels, influence both disease stage and survival after diagnosis with colorectal cancer (CRC); low levels of expression may be background noise. We examine 304 infrequently expressed miRNAs in 1893 population-based cases of CRC with paired carcinoma and normal mucosa miRNA profiles...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29128931/universal-determination-of-microsatellite-instability-using-bat26-as-a-single-marker-in-an-argentine-colorectal-cancer-cohort
#13
María Laura González, Natalia Causada-Calo, Juan Pablo Santino, Mev Dominguez-Valentin, Fabiana Alejandra Ferro, Inés Sammartino, Pablo Germán Kalfayan, Maria Alicia Verzura, Tamara Alejandra Piñero, Andrea Romina Cajal, Walter Pavicic, Carlos Vaccaro
Microsatellite instability (MSI) is a hallmark tool for Lynch syndrome (LS) screening and a prognostic marker for sporadic colorectal cancer (CRC). In regions with limited resources and scarce CRC molecular characterization as South America, the implementation of universal MSI screening is under debate for both its purposes. We sought to estimate the frequency of BAT26 in colorectal adenocarcinomas and to determine associated clinical and histological features. Consecutive patients from a CRC registry were included...
November 11, 2017: Familial Cancer
https://www.readbyqxmd.com/read/29126141/gastric-carcinomas-with-lymphoid-stroma-categorization-and-comparison-with-solid-type-colonic-carcinomas
#14
Raul S Gonzalez, Justin M M Cates, Frank Revetta, Loralee A McMahon, Kay Washington
Objectives: To determine whether histologic features could help identify gastric carcinomas with lymphoid stroma associated with microsatellite instability (MSI) (ie, "medullary carcinomas"), Epstein-Barr virus (EBV) infection (termed lymphoepithelioma-like carcinomas in other organ systems), or neither. Methods: We identified 17 solid-type gastric carcinomas with lymphoid stroma, assessed EBV and MSI status, and compared features across groups. We also compared them with 51 solid-type colorectal adenocarcinomas...
November 8, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29123583/medullary-colonic-carcinoma-with-microsatellite-instability-has-lower-survival-compared-with-conventional-colonic-adenocarcinoma-with-microsatellite-instability
#15
Miguel A Gómez-Álvarez, Leonardo S Lino-Silva, Rosa A Salcedo-Hernández, Alejandro Padilla-Rosciano, Erika B Ruiz-García, Horacio N López-Basave, German Calderillo-Ruiz, José M Aguilar-Romero, Jorge A Domínguez-Rodríguez, Ángel Herrera-Gómez, Abelardo Meneses-García
Introduction: Colorectal medullary carcinoma (MC) is a rare subtype of poorly differentiated adenocarcinoma (PDA) with unclear prognostic significance. Microsatellite instable (MSI) colorectal carcinomas have demonstrated better prognosis in clinical stage II. Aim: To analyze the survival and clinicopathological characteristics of MCs versus PDAs with MSI in clinical stage III. Material and methods: We studied 22 cases of PDAs with MSI versus 10 MCs...
2017: Przegla̜d Gastroenterologiczny
https://www.readbyqxmd.com/read/29106440/identification-of-pmn-released-mutagenic-factors-in-a-co-culture-model-for-colitis-associated-cancer
#16
Nicolas Granofszky, Michaela Lang, Vineeta Khare, Gerald Schmid, Theresa Scharl, Franziska Ferk, Kristine Jimenez, Siegfried Knasmüller, Christoph Campregher, Christoph Gasche
Microsatellite instability (MSI) is present in ulcerative colitis and colitis-associated colorectal cancers. Certain factors released by polymorphonuclear cells (PMN) may drive mucosal frameshift mutations resulting in MSI and cancer. Here, we applied a co-culture system with PMNs and colon epithelial cells to identify such culprit factors. Subjecting HCT116+chr3 and HCEC-1CT MSI-reporter cell lines harboring mono-, di- or tetranucleotide DNA repeats linked to EGFP to activated PMNs induced frameshift mutations within all repeats, as quantified by flow cytometry...
November 2, 2017: Carcinogenesis
https://www.readbyqxmd.com/read/29105220/correlative-mass-spectrometry-imaging-applying-tof-sims-and-ap-maldi-to-a-single-tissue-section
#17
N Desbenoit, A Walch, B Spengler, A Brunelle, A Römpp
RATIONALE: Mass spectrometry imaging (MSI) is a powerful tool for mapping the surface of a sample. Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization (AP-MALDI) offer complementary capabilities. Here, we present a workflow to apply both techniques to a single tissue section and combine the resulting data on the example of human colon cancer tissue. METHODS: Following cryo-sectioning, images were acquired using the high spatial resolution (1 μm pixel size) provided by TOF-SIMS...
November 3, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29038296/a-blueprint-to-advance-colorectal-cancer-immunotherapies
#18
Dung T Le, Vanessa M Hubbard-Lucey, Michael A Morse, Christopher R Heery, Andrea Dwyer, Thomas H Marsilje, Arthur N Brodsky, Emily Chan, Dustin A Deming, Luis A Diaz, Wolf H Fridman, Richard M Goldberg, Stanley R Hamilton, Franck Housseau, Elizabeth M Jaffee, S Peter Kang, Smitha S Krishnamurthi, Christopher H Lieu, Wells Messersmith, Cynthia L Sears, Neil H Segal, Arvin Yang, Rebecca A Moss, Edward Cha, Jill O'Donnell-Tormey, Nancy Roach, Anjelica Q Davis, Keavy McAbee, Sharyn Worrall, Al B Benson
Immunotherapy is rapidly becoming a standard of care for many cancers. However, colorectal cancer had been generally resistant to immunotherapy, despite features in common with sensitive tumors. Observations of substantial clinical activity for checkpoint blockade in colorectal cancers with defective mismatch repair (microsatellite instability-high tumors) have reignited interest in the search for immunotherapies that could be extended to the larger microsatellite stable (MSS) population. The Cancer Research Institute and Fight Colorectal Cancer convened a group of scientists, clinicians, advocates, and industry experts in colorectal cancer and immunotherapy to compile ongoing research efforts, identify gaps in translational and clinical research, and provide a blueprint to advance immunotherapy...
October 16, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28989593/colorectal-cancer-screening-a-comprehensive-review-to-recent-non-invasive-methods
#19
REVIEW
Leila Hamzehzadeh, Meysam Yousefi, Seyed-Hamidollah Ghaffari
Colorectal cancer (CRC) is one of the most common cancers worldwide and considered to be one of the hassles in medical communities. CRC develops from precancerous polyps in the colon or rectum and is preventable and curable by an early diagnosis and with the removal of premalignant polyps. In recent years, scientists have looked for inexpensive and safe ways to detect CRC in its earliest stages. Strong evidence shows that screening for CRC is a crucial way to reduce the incidence and mortality of this devastating disease...
July 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
https://www.readbyqxmd.com/read/28957395/high-mrna-expression-of-splice-variant-syk-short-correlates-with-hepatic-disease-progression-in-chemonaive-lymph-node-negative-colon-cancer-patients
#20
MULTICENTER STUDY
Robert R J Coebergh van den Braak, Anieta M Sieuwerts, Raju Kandimalla, Zarina S Lalmahomed, Sandra I Bril, Anne van Galen, Marcel Smid, Katharina Biermann, J Han J M van Krieken, Wigard P Kloosterman, John A Foekens, Ajay Goel, John W M Martens, Jan N M IJzermans
OBJECTIVE: Overall and splice specific expression of Spleen Tyrosine Kinase (SYK) has been posed as a marker predicting both poor and favorable outcome in various epithelial malignancies. However, its role in colorectal cancer is largely unknown. The aim of this study was to explore the prognostic role of SYK in three cohorts of colon cancer patients. METHODS: Total messenger RNA (mRNA) expression of SYK, SYK(T), and mRNA expression of its two splice variants SYK short (S) and SYK long (L) were measured using quantitative reverse transcriptase (RT-qPCR) in 240 primary colon cancer patients (n = 160 patients with chemonaive lymph node negative [LNN] and n = 80 patients with adjuvant treated lymph node positive [LNP] colon cancer) and related to microsatellite instability (MSI), known colorectal cancer mutations, and disease-free (DFS), hepatic metastasis-free (HFS) and overall survival (OS)...
2017: PloS One
keyword
keyword
111143
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"