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Lactate AND PPAR gamma

Seung-Hwan Moon, Su Jin Lee, Kyung-Ho Jung, Cung Hoa Thien Quach, Jin-Won Park, Jin Hee Lee, Young Seok Cho, Kyung-Han Lee
UNLABELLED: We evaluated how troglitazone influences cancer cell glucose metabolism and uptake of (18)F-FDG, and we investigated its molecular mechanism and relation to the drug's anticancer effect. METHODS: Human T47D breast and HCT116 colon cancer cells that had been treated with troglitazone were measured for (18)F-FDG uptake, lactate release, oxygen consumption rate, mitochondrial membrane potential, and intracellular reactive oxygen species. Viable cell content was measured by sulforhodamine-B assays...
January 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Soundharrajan Ilavenil, Da Hye Kim, Mariadhas Valan Arasu, Srisesharam Srigopalram, Ravikumar Sivanesan, Ki Choon Choi
Synthetic drugs are commonly used to cure various human ailments at present. However, the uses of synthetic drugs are strictly regulated because of their adverse effects. Thus, naturally occurring molecules may be more suitable for curing disease without unfavorable effects. Therefore, we investigated phenyllactic acid (PLA) from Lactobacillus plantarum with respect to its effects on adipogenic genes and their protein expression in 3T3-L1 pre-adipocytes by qPCR and western blot techniques. PLA enhanced differentiation and lipid accumulation in 3T3-L1 cells at the concentrations of 25, 50, and 100 μM...
2015: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jaroslav Zelenka, Aleš Dvořák, Lukáš Alán
A moderate elevation of reactive oxygen species (ROS) production and a mild inhibition of mitochondrial respiratory chain have been associated with a health promotion and a lifespan extension in several animal models of aging. Here, we tested whether this phenomenon called mitohormesis could be mediated by L-lactate. The treatment with 5 mM L-lactate significantly increased H2O2 production and slightly inhibited the respiration in cultured skin fibroblasts and in isolated mitochondria. The L-lactate exposure was associated with oxidation of intracellular glutathione, phosphorylation of 5'AMP-activated protein kinase (AMPK), and induction of peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) transcription...
2015: Oxidative Medicine and Cellular Longevity
Grégory Pourié, Nicolas Martin, Carine Bossenmeyer-Pourié, Nassila Akchiche, Rosa Maria Guéant-Rodriguez, Andréa Geoffroy, Elise Jeannesson, Sarah El Hajj Chehadeh, Khalid Mimoun, Patrick Brachet, Violette Koziel, Jean-Marc Alberto, Deborah Helle, Renée Debard, Brigitte Leininger, Jean-Luc Daval, Jean-Louis Guéant
Deficiency in the methyl donors vitamin B12 and folate during pregnancy and postnatal life impairs proper brain development. We studied the consequences of this combined deficiency on cerebellum plasticity in offspring from rat mothers subjected to deficient diet during gestation and lactation and in rat neuroprogenitor cells expressing cerebellum markers. The major proteomic change in cerebellum of 21-d-old deprived females was a 2.2-fold lower expression of synapsins, which was confirmed in neuroprogenitors cultivated in the deficient condition...
September 2015: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Sílvia Regina de Lima Reis, Naoel Hassan Feres, Leticia Martins Ignacio-Souza, Roberto Vilela Veloso, Vanessa Cristina Arantes, Nair Honda Kawashita, Edson Moleta Colodel, Bárbara Laet Botosso, Marise Auxiliadora de Barros Reis, Márcia Queiroz Latorraca
We evaluated the effects of postweaning nutritional recovery with a soybean flour diet on de novo hepatic lipogenesis and inflammation in adult rats exposed to protein restriction during intrauterine life and lactation. Rats from mothers fed with protein (casein) in a percentage of 17% (control, C) or 6% (low, L) during pregnancy and lactation were fed with diet that contained 17% casein (CC and LC groups, resp.) or soybean (CS and LS groups, resp.) after weaning until 90 days of age. LS and CS rats had low body weight, normal basal serum triglyceride levels, increased ALT concentrations, and high HOMA-IR indices compared with LC and CC rats...
2015: Mediators of Inflammation
D Lifanov, M N Khadyeva, L Sh Rahmatullina, S V Demenev, R R Ibragimov
The aim of this study was to evaluate the effects of short-term creatine supplementation on exercise performance in male athletes depending on the studied genotypes. The present study was limited as long as to two common polymorphisms, such as C34T AMPD1 and Pro12Ala PPARG, selected because previously reported these associations with various aspects of metabolic abnormalities. Athletes had significantly higher frequency of T allele compared to controls AMPD1 34T (7.9 vs. 15.6 %, p < 0.0001) and PPARG 12Ala allele compared to controls (20...
June 2014: Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova
Zheng Jia, Xiao Xinhua, Zhang Qian, Yu Miao, Xu Jianping, Wang Zhixin, Liu Yijing, Li Mingmin
Peroxisome proliferator-activated receptors (PPARs) are a group of nuclear receptor proteins that regulate gene transcription. PPARs play essential roles in modulating cell differentiation, development, and metabolism (carbohydrate, lipid, protein). Here, we investigated whether PPARγ plays a role in linking maternal malnutrition and aberrant metabolism in the offspring of mice. After feeding dams with high fat (HF) and low protein (LP) diet during pregnancy and lactation, we examined the effects on the offspring at weaning (age of 3-week)...
January 2015: Yi Chuan, Hereditas
Jiangjiang Zhu, Yuting Sun, Jun Luo, Min Wu, Jianhua Li, Yanhong Cao
Specificity protein 1 (SP1) is a ubiquitous transcription factor that plays an important role in controlling gene expression. Although important in mediating the function of various hormones, the role of SP1 in regulating milk fat formation remains unknown. To investigate the sequence and expression information, as well as its role in modulating lipid metabolism, we cloned SP1 gene from mammary gland of Xinong Saanen dairy goat. The full-length cDNA of the SP1 gene is 4376 bp including 103 bp of 5'UTR, 2358 bp of ORF (HM_236311) and 1915 bp of 3'UTR, which is predicted to encode a 786 amino acids polypeptide...
2015: International Journal of Molecular Sciences
Hylde Zirpoli, Mariane Abdillahi, Nosirudeen Quadri, Radha Ananthakrishnan, Lingjie Wang, Rosa Rosario, Zhengbin Zhu, Richard J Deckelbaum, Ravichandran Ramasamy
Dietary n-3 fatty acids (FAs) may reduce cardiovascular disease risk. We questioned whether acute administration of n-3 rich triglyceride (TG) emulsions could preserve cardiac function and decrease injury after ischemia/reperfusion (I/R) insult. We used two different experimental models: in vivo, C57BL/6 mice were exposed to acute occlusion of the left anterior descending coronary artery (LAD), and ex-vivo, C57BL/6 murine hearts were perfused using Langendorff technique (LT). In the LAD model, mice treated with n-3 TG emulsion (1...
2015: PloS One
Priscila P Santos, Fernando Oliveira, Vanessa C M P Ferreira, Bertha F Polegato, Meliza G Roscani, Ana Angelica Fernandes, Pamela Modesto, Bruna P M Rafacho, Silmeia G Zanati, Annarita Di Lorenzo, Luiz S Matsubara, Sergio A R Paiva, Leonardo A M Zornoff, Marcos F Minicucci, Paula S Azevedo
BACKGROUND/AIMS: Experimental and clinical studies have shown the direct toxic effects of cigarette smoke (CS) on the myocardium, independent of vascular effects. However, the underlying mechanisms are not well known. METHODS: Wistar rats were allocated to control (C) and cigarette smoke (CS) groups. CS rats were exposed to cigarette smoke for 2 months. RESULTS: After that morphometric, functional and biochemical parameters were measured. The echocardiographic study showed enlargement of the left atria, increase in the left ventricular systolic volume and reduced systolic function...
2014: PloS One
Eleni Tsiplakou, Emmanouil Flemetakis, Evangelia-Diamanto Kouri, Kyriaki Sotirakoglou, George Zervas
Milk fatty acid (FA) synthesis by the mammary gland involves expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, we examined the effect of long-term under- and over-feeding on the expression of genes (acetyl Co A carboxylase, ACC; fatty acid synthetase, FAS; lipoprotein lipase, LPL; stearoyl Co A desaturase, SCD; peroxisome proliferator activated receptor γ2, PPARγ2; sterol regulatory element binding protein-1, SREBP-1c; and hormone sensitive lipase, HSL) related to FA metabolism in sheep mammary tissue (MT)...
February 2015: Journal of Dairy Research
Yogeeta O Agrawal, Pankaj Kumar Sharma, Birendra Shrivastava, Shreesh Ojha, Harshita M Upadhya, Dharamvir Singh Arya, Sameer N Goyal
The present study investigated the effect of hesperidin, a natural flavonoid, in cardiac ischemia and reperfusion (I/R) injury in diabetic rats. Male Wistar rats with diabetes were divided into five groups and were orally administered saline once daily (IR-sham and IR-control), Hesperidin (100 mg/kg/day; IR-Hesperidin), GW9962 (PPAR-γ receptor antagonist), or combination of both for 14 days. On the 15th day, in the IR-control and IR-treatment groups, rats were subjected to left anterior descending (LAD) coronary artery occlusion for 45 minutes followed by a one-hour reperfusion...
2014: PloS One
Gang Shu, Nai-Sheng Lu, Xiao-Tong Zhu, Yong Xu, Min-Qing Du, Qiu-Ping Xie, Can-Jun Zhu, Qi Xu, Song-Bo Wang, Li-Na Wang, Ping Gao, Qian-Yun Xi, Yong-Liang Zhang, Qing-Yan Jiang
Adipocyte dysfunction is associated with many metabolic diseases such as obesity, insulin resistance and diabetes. Previous studies found that phloretin promotes 3T3-L1 cells differentiation, but the underlying mechanisms for phloretin's effects on adipogenesis remain unclear. In this study, we demonstrated that phloretin enhanced the lipid accumulation in porcine primary adipocytes in a time-dependent manner. Furthermore, phloretin increased the utilization of glucose and nonesterified fatty acid, while it decreased the lactate output...
December 2014: Journal of Nutritional Biochemistry
Jie Wang, Yanjie Bian, Zhuoran Wang, Dan Li, Chunmei Wang, Qingzhang Li, Xuejun Gao
MicroRNAs (miRNAs) are a class of small non-coding, endogenous regulatory RNAs that function by controlling gene expression at the post-transcriptional level. Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1), which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows...
2014: PloS One
Zun-Jing Liu, Hong-Qiang Liu, Cheng Xiao, Hui-Zhen Fan, Qing Huang, Yun-Hai Liu, Yu Wang
The turmeric derivative curcumin protects against cerebral ischemic injury. We previously demonstrated that curcumin activates peroxisome proliferator-activated receptor-γ (PPARγ), a ligand-activated transcription factor involved in both neuroprotective and anti-inflammatory signaling pathways. This study tested whether the neuroprotective effects of curcumin against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced injury of rat cortical neurons are mediated (at least in part) by PPARγ. Curcumin (10 μM) potently enhanced PPARγ expression and transcriptional activity following OGD/R...
November 2014: Journal of Neuroscience Research
Yogeeta O Agrawal, Pankaj Kumar Sharma, Birendra Shrivastava, Dharamvir Singh Arya, Sameer N Goyal
Hesperidin has been shown to possess cardioprotective and anti-diabetic potential. Hitherto, its molecular mechanism on isoproterenol (ISO)-induced myocardial dysfunction in diabetes is still not explored. Hence, for the first time we sought to investigate whether hesperidin exerts any beneficial effect on the pathophysiology of myocardial infarction (MI) in diabetes through the PPAR-γ pathway by assessing a variety of indices e.g., apoptosis, hemodynamic, biochemical and histoarchitectural changes. Diabetes was induced by a single dose of STZ (50 mg/kg IP)...
August 5, 2014: Chemico-biological Interactions
M Ilamathi, S Senthilkumar, P C Prabu, S Panchapakesan, V Sivaramakrishnan
The salubrious effects of 3-Formylchromone (3-FC) against nitrosodiethylamine (NDEA) mediated early hepatocellular carcinogenesis was investigated in vivo by this study. Male Wistar rats were administered three interspersed intraperitoneal injections of NDEA (200 mg/kg body weight) until sixth week, followed by, thrice a week oral dose of 3-FC (25 mg/kg body weight) from the seventh week to eleventh week. Oral supplementation of Wistar rats with 3-FC prevented the increase in serum marker enzymes (AST, ALT, LDH) and serum pre-neoplastic marker (γ-GT) induced by NDEA...
August 5, 2014: Chemico-biological Interactions
Huaiping Shi, Hengbo Shi, Jun Luo, Wei Wang, Abiel B Haile, Huifen Xu, Jun Li
Although research on dairy goat mammary gland have referred extensively to molecular mechanisms, research on lines of dairy goat mammary epithelial cells (MECs) are still rare. This paper sought to establish an immortal MEC line by stable transfection of human telomerase. MECs from a lactating (45 days post-parturition) Xinong Saanen dairy goat were cultured purely and subsequently transfected with a plasmid carrying the sequence of human telomerase. Immortalized MECs by human telomerase (hT-MECs) exhibited a typical cobblestone morphology and activity and expression levels of telomerase resembled that of MCF-7 cells...
July 2014: Animal Science Journal, Nihon Chikusan Gakkaihō
Audrey Carrière, Yannick Jeanson, Sandra Berger-Müller, Mireille André, Vanessa Chenouard, Emmanuelle Arnaud, Corinne Barreau, Romy Walther, Anne Galinier, Brigitte Wdziekonski, Phi Villageois, Katie Louche, Philippe Collas, Cédric Moro, Christian Dani, Francesc Villarroya, Louis Casteilla
The presence of brown adipose tissue (BAT) in human adults opens attractive perspectives to treat metabolic disorders. Indeed, BAT dissipates energy as heat via uncoupling protein (UCP)1. Brown adipocytes are located in specific deposits or can emerge among white fat through the so-called browning process. Although numerous inducers have been shown to drive this process, no study has investigated whether it could be controlled by specific metabolites. Here, we show that lactate, an important metabolic intermediate, induces browning of murine white adipose cells with expression of functional UCP1...
October 2014: Diabetes
Diwakar Vyas, Beverly B Teter, Pierluigi Delmonte, Richard A Erdman
Our objective was to investigate the combination of rosiglitazone (ROSI) and conjugated linoleic acid (CLA) on mammary and hepatic lipogenesis in lactating C57Bl/6 J mice. Twenty-four lactating mice were randomly assigned to one of four treatments applied from postpartum day 6 to day 10. Treatments included: (1) control diet, (2) control plus 1.5 % dietary CLA (CLA) substituted for soybean oil, (3) control plus daily intra-peritoneal (IP) rosiglitazone injections (10 mg/kg body weight) (ROSI), and (4) CLA plus ROSI (CLA-ROSI)...
July 2014: Lipids
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