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Lysophosphatidic acid

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https://www.readbyqxmd.com/read/29226557/lysophosphatidic-acid-accelerates-development-of-porcine-embryos-by-activating-formation-of-the-blastocoel
#1
Min-Young Shin, Seung-Eun Lee, Yeo-Jin Son, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Culture media modifications, including the addition of various factors, are important for the in vitro production of oocytes and embryos. In this study, we investigated the effects of lysophosphatidic acid (LPA) on porcine embryo development. Porcine parthenogenetic embryos were cultured with 0, 0.1, 1, and 10 μM LPA for 7 days, or cultured in basic medium until Day 4 and then treated with LPA from Days 4 to 7. No difference in the in vitro development of embryos cultured with LPA for 7 days was observed. Conversely, rates of blastocyst and over-expanded blastocyst formation were higher in the 0...
December 11, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29217823/lysophosphatidic-acid-protects-against-acetaminophen-induced-acute-liver-injury
#2
Geon Ho Bae, Sung Kyun Lee, Hyung Sik Kim, Mingyu Lee, Ha Young Lee, Yoe-Sik Bae
We investigated the effect of lysophosphatidic acid (LPA) in experimental acetaminophen (APAP)-induced acute liver injury. LPA administration significantly reduced APAP-challenged acute liver injury, showing attenuated liver damage, liver cell death and aspartate aminotransferase and alanine aminotransferase levels. APAP overdose-induced mortality was also significantly decreased by LPA administration. Regarding the mechanism involved in LPA-induced protection against acute liver injury, LPA administration significantly increased the glutathione level, which was markedly decreased in APAP challenge-induced acute liver injury...
December 8, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29211777/alpha-conotoxin-buia-globular-isomer-is-a-competitive-antagonist-for-oleoyl-l-alpha-lysophosphatidic-acid-binding-to-lpar6-a-molecular-dynamics-study
#3
Saima Younis, Sajid Rashid
Lysophosphatidic acid receptor 6 (LPAR6) is a G-protein coupled receptor (GPCR) involved in hair development and cytoskeleton formation in mammals. Its proliferation is implicated in several forms of cancer including liver cancer, squamous cell carcinoma and metastatic prostate cancer. Current study emphasizes the isolation of competitive non-lipid and stable peptide antagonists for Lysophosphatidic acid ligand. A total of 148 conotoxin structures were characterized for their binding abilities against LPAR6...
2017: PloS One
https://www.readbyqxmd.com/read/29211497/the-rho-kinase-isoforms-rock1-and-rock2-each-contribute-to-the-development-of-experimental-pulmonary-fibrosis
#4
Rachel S Knipe, Clemens K Probst, David Lagares, Alicia Franklin, Jillian J Spinney, Patricia L Brazee, Paula Grasberger, Linlin Zhang, Katharine E Black, Norihiko Sakai, Barry S Shea, James K Liao, Benjamin D Medoff, Andrew M Tager
Pulmonary fibrosis is thought to result from dysregulated wound repair after repetitive lung injury. Many cellular responses to injury involve rearrangements of the actin cytoskeleton mediated by the two isoforms of the Rho-associated coiled coil forming protein kinase, ROCK1 and ROCK2. Additionally, profibrotic mediators such as transforming growth factor-beta (TGF-β), thrombin and lysophosphatidic acid (LPA) act through receptors that activate ROCK. Inhibition of ROCK activation may be a potent therapeutic strategy for human pulmonary fibrosis...
December 6, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29209219/differential-effects-of-inhibitor-combinations-on-lysophosphatidic-acid-mediated-chemokine-secretion-in-unprimed-and-tumor-necrosis-factor-%C3%AE-primed-synovial-fibroblasts
#5
Weili Hui, Chenqi Zhao, Sylvain G Bourgoin
Lysophosphatidic acid (LPA) is a pleiotropic bioactive lysophospholipid involved in inflammatory mediator synthesis. Signaling through p38MAPK, ERK, Rho kinase, and MSK-CREB contributes to LPA-mediated IL-8 production in fibroblast-like synoviocytes (FLS) from rheumatoid arthritis (RA) patients. The study was undertaken to investigate how LPA activates MSKs and how signaling crosstalk between TNFα and LPA contributes to the super-production of cytokines/chemokines. RAFLS pretreated or not with TNFα were stimulated with LPA...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29208511/identification-of-potent-lysophosphatidic-acid-receptor-5-lpa5-antagonists-as-potential-analgesic-agents
#6
Yuichiro Kawamoto, Ryushi Seo, Nobuhito Murai, Hideki Hiyama, Hiromasa Oka
Lysophosphatidic acid (LPA) plays an important role in a variety of cellular functions. In particular, LPA5 receptor is highly expressed in spinal cord and dorsal root ganglion, which are associated with pain. This fact prompted us to hypothesize that LPA5 antagonists show analgesic effects. To search for potent LPA5 antagonists with blood brain barrier (BBB) permeability, we conducted high throughput screening (HTS). In HTS campaign, we found a 2H-isoquinoline-1-one scaffold showing antagonistic activity against LPA5 and synthesized a series of 2H-isoquinoline-1-one derivatives and evaluated their LPA5 activities...
November 26, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29198709/cholesterol-inhibits-entotic-cell-in-cell-formation-and-actomyosin-contraction
#7
Banzhan Ruan, Bo Zhang, Ang Chen, Long Yuan, Jianqing Liang, Manna Wang, Zhengrong Zhang, Jie Fan, Xiaochen Yu, Xin Zhang, Zubiao Niu, You Zheng, Songzhi Gu, Xiaoqing Liu, Hongli Du, Jufang Wang, Xianwen Hu, Lihua Gao, Zhaolie Chen, Hongyan Huang, Xiaoning Wang, Qiang Sun
Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cell-in-cell structures by utilizing liposome as a vector. We found that Lipofectamine-2000, the reagent commonly used for routine transfection, could significantly reduce entotic cell-in-cell formation in a cell-specific manner, which is correlated with suppressed actomyosin contraction as indicated by reduced β-actin expression and myosin light chain phosphorylation...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29197066/characterisation-of-the-properties-of-a-selective-orally-bioavailable-autotaxin-inhibitor-in-preclinical-models-of-advanced-stages-of-liver-fibrosis
#8
Manuel Baader, Tom Bretschneider, Andre Broermann, Joerg F Rippmann, Birgit Stierstorfer, Christian A Kuttruff, Michael Mark
BACKGROUND AND PURPOSE: Autotaxin (ATX) is a secreted phospholipase which hydrolyses lysophosphatidylcholine (LPC) to generate lysophosphatidic acid (LPA). The extracellular signalling molecule LPA exerts its biological actions through activation of six G protein-coupled receptors expressed in various cell types including fibroblasts. Multiple preclinical studies using knockout animals, LPA receptor antagonists or ATX inhibitors have provided evidence for a potential role of the ATX/LPA axis in tissue fibrosis...
December 2, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29182668/extracellular-vesicles-released-by-fibroblasts-undergoing-h-ras-induced-senescence-show-changes-in-lipid-profile
#9
Sandra Buratta, Lorena Urbanelli, Krizia Sagini, Stefano Giovagnoli, Silvia Caponi, Daniele Fioretto, Nico Mitro, Donatella Caruso, Carla Emiliani
Cells release extracellular vesicles (EVs) in their environment and cellular lipids play an important role in their formation, secretion and uptake. Besides, there is also evidence that EV transferred lipids impact on recipient's cell signaling. Cellular senescence is characterized by a state of permanent proliferation arrest and represents a barrier towards the development of neoplastic lesions. A peculiar feature of senescence is the release of many soluble factors, the so-called Senescence-Associated Secretory Phenotype, which play a key role in triggering paracrine senescence signals...
2017: PloS One
https://www.readbyqxmd.com/read/29182028/altered-expression-of-lysophosphatidic-acid-receptors-in-association-with-the-synthesis-of-estrogens-and-androgens-in-type-1-endometrial-cancer-biology
#10
Tomasz Wasniewski, Izabela Woclawek-Potocka
To establish association between two main lysophosphatidic acid (LPA) receptors (LPAR2 and LPAR1) with the synthesis of estrogens and androgens in type-1 endometrial carcinoma (EC), we evaluated correlation of LPARs expression with expression of steroid 5 alpha-reductase 2 - aromatase (SRD5A2), or cytochrome P450 family 19 subfamily A member 1-5α-reductase (CYP19A1) in EC. Moreover, we aimed to investigate SRD5A2 and CYP19A1 expression in type 1 endometrial cancer and normal endometrium with its correlation to selected clinicopathological features...
November 28, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29178922/elevated-plasma-levels-of-lysophosphatidic-acid-and-aberrant-expression-of-lysophosphatidic-acid-receptors-in-adenomyosis
#11
Bicheng Yang, Liqun Wang, Xiaoju Wan, Yunjun Li, Xiaohong Yu, Yunna Qin, Yong Luo, Feng Wang, Ouping Huang
BACKGROUND: Given the important roles of the receptor-mediated lysophosphatidic acid (LPA) signaling in both reproductive tract function and gynecological cancers, it will be informative to investigate the potential role of LPA in the development of adenomyosis. The objective of this study was to evaluate the levels of LPA in plasma and the expression of six LPA receptors in the endometrial tissue collected from women with and without adenomyosis. METHODS: Plasma and endometrial tissue samples were collected form women with and without adenomyosis...
November 25, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/29170807/intracellular-lysophosphatidic-acid-influences-cell-migration
#12
EDITORIAL
Regina Stöber
No abstract text is available yet for this article.
November 24, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29167463/structural-insights-into-the-committed-step-of-bacterial-phospholipid-biosynthesis
#13
Zhenjian Li, Yannan Tang, Yiran Wu, Suwen Zhao, Juan Bao, Yitian Luo, Dianfan Li
The membrane-integral glycerol 3-phosphate (G3P) acyltransferase PlsY catalyses the committed and essential step in bacterial phospholipid biosynthesis by acylation of G3P, forming lysophosphatidic acid. It contains no known acyltransferase motifs, lacks eukaryotic homologs, and uses the unusual acyl-phosphate as acyl donor, as opposed to acyl-CoA or acyl-carrier protein for other acyltransferases. Previous studies have identified several PlsY inhibitors as potential antimicrobials. Here we determine the crystal structure of PlsY at 1...
November 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/29162771/in-vivo-evidence-for-possible-up-regulating-roles-of-lysophosphatidic-acid-around-fertilization-in-rats
#14
Tae Takeda, Miki Shirasaka, Makoto Sugiyama, Ryota Terashihma, Mitsumori Kawaminami, Shiro Kurusu
Lysophosphatidic acid (LPA) produced by autotaxin (ATX) is recognized as a multi-functional mediator in mammalian reproduction. This study focused on possible effect(s) of LPA on ovulated cumulus-oocyte complexes (COCs) around fertilization in rats in vivo. Immunohistochemistry revealed the cell-type-dependent localization of candidates of synthetic enzymes, ATX and two phospholipases A2 isofroms, and LPA receptors LPA1~4 in ovulated COCs and in oviductal epithelium. The eggs ovulated with a form of COCs became denuded of cumulus cells and underwent fragmentation in the absence of fertilization...
November 21, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29147686/lipoprotein-a-associated-molecules-are-prominent-components-in-plasma-and-valve-leaflets-in-calcific-aortic-valve-stenosis
#15
Michael Torzewski, Amir Ravandi, Calvin Yeang, Andrea Edel, Rahul Bhindi, Stefan Kath, Laura Twardowski, Jens Schmid, Xiaohong Yang, Ulrich F W Franke, Joseph L Witztum, Sotirios Tsimikas
The LPA gene is the only monogenetic risk factor for calcific aortic valve stenosis (CAVS). Oxidized phospholipids (OxPL) and lysophosphatidic acid generated by autotaxin (ATX) from OxPL are pro-inflammatory. Aortic valve leaflets were categorized pathologically from Both ATX-apoB and ATX-apo(a) were measureable in plasma. Lp(a), autotaxin, OxPL and MDA epitopes progressively increased in immunostaining (p<0.001 for all). Six species of OxPL and LysoPA were identified following extraction from valve leaflets...
June 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/29145606/-prokaryotic-pathway-is-not-prokaryotic-non-cyanobacterial-origin-of-the-chloroplast-lipid-biosynthetic-pathway-revealed-by-comprehensive-phylogenomic-analysis
#16
Naoki Sato, Koichiro Awai
Lipid biosynthesis within the chloroplast, or more generally plastids, was conventionally called "prokaryotic pathway", which produces glycerolipids bearing C18 acids at the sn-1 position and C16 acids at the sn-2 position, as in cyanobacteria such as Anabaena and Synechocystis. This positional specificity is determined during the synthesis of phosphatidate, which is a precursor to diacylglycerol, the acceptor of galactose for the synthesis of galactolipids. The first acylation at sn-1 is catalyzed by glycerol-3-phosphate acyltransferase (GPAT or GPT), whereas the second acylation at sn-2 is performed by lysophosphatidate acyltransferase (LPAAT, AGPAT or PlsC)...
November 14, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29143552/metabonomics-analysis-of-serum-from-rats-given-long-term-and-low-level-cadmium-by-ultra-performance-liquid-chromatography-mass-spectrometry
#17
Liyan Hu, Lu Bo, Meiyan Zhang, Siqi Li, Xiujuan Zhao, Changhao Sun
1. This study evaluated the toxicity of chronic exposure to low-level cadmium (Cd) in rats using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Forty male Sprague-Dawley rats were randomly assigned to four groups, namely, the control group, low-dose group (0.13 mg/kg·bw), middle-dose group (0.8 mg/kg·bw) and high-dose group (4.89 mg/kg·bw). The rats continuously received CdCl2 via drinking water for 24 weeks. Serum samples were collected for metabonomics analysis. The data generated from the UPLC-MS was analysed using principal components analysis (PCA) and partial least-squares discriminant analysis (PLS-DA)...
November 16, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29128569/lysophosphatidic-acid-receptor-1-is-important-for-intestinal-epithelial-barrier-function-and-susceptibility-to-colitis
#18
Songbai Lin, Yiran Han, Kayte Jenkin, Sei-Jung Lee, Maiko Sasaki, Jan-Michael Klapproth, Peijian He, C Chris Yun
Intestinal epithelial cells form a barrier that is critical in protecting the host from the hostile luminal environment. Previously, we showed that lysophosphatidic acid receptor 1 (LPAR1) regulates proliferation of intestinal epithelial cells such that the absence of LPA1 mitigates epithelial wound healing process. This study provides evidence that LPA1 is important for the maintenance of epithelial barrier integrity. The epithelial permeability, determined by fluorescently labeled dextran flux and transepithelial resistance, is increased in Lpar1(-/-) (Lpa1(-/-)) mouse intestine...
November 8, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29127342/chemical-analysis-of-morphological-changes-in-lysophosphatidic-acid-treated-ovarian-cancer-cells
#19
Karen A Bailey, Yuliya Klymenko, Peter E Feist, Amanda B Hummon, M Sharon Stack, Zachary D Schultz
Ovarian cancer (OvCa) cells are reported to undergo biochemical changes at the cell surface in response to treatment with lysophosphatidic acid (LPA). Here we use scanning electron microscopy (SEM) and multiplex coherent anti-Stokes Raman scattering (CARS) imaging via supercontinuum excitation to probe morphological changes that result from LPA treatment. SEM images show distinct shedding of microvilli-like features upon treatment with LPA. Analysis of multiplex CARS images can distinguish between molecular components, such as lipids and proteins...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29081100/-the-clinical-study-on-the-correlation-of-lysophosphatidic-acid-and-vascular-endotheli-growth-factor-and-endothelin-in-serum-of-pneumoconiosis
#20
L J Mao, Y L Zhang, C C Cong, L Guan, S Q Li, J Y Zhao
Objective: To explore the role of lysophosphatidic acid, vascular endothelial growth facor and endothelin in the pathogenesis of pulmonary fibrosis in coal workers'pneumoconiosis patients, the relationship of lysophosphatidic acid, VEGF and ET in serum was studied. Methods: Sixty two pneumoconiosis patients were selected as cases group, which included 23 cases of stage Ⅰ, 25 cases of stageⅡand 14 cases of stageⅢ. Twenty workers were selected as dust exposure group who exposed to coal dust for more than 2 years and had not been diagnosed as pneumoconiosis...
July 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
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