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https://www.readbyqxmd.com/read/29774455/semaphorin-3a-promotes-osteogenic-differentiation-of-bmsc-from-type-2-diabetes-mellitus-rats
#1
Qiao Qiao, Xiaoru Xu, Yingliang Song, Shuang Song, Wenzhong Zhu, Fenglan Li
Bone regeneration is impaired in patients with type 2 diabetes mellitus (T2DM), which leads to non-healing after bone loss. The decreased osteogenic capacity of bone mesenchymal stem cells (BMSCs) might be a main reason. Sema3A, as a powerful protein promoting osteocyte differentiation, shows potential for bone regeneration treatment. BMSCs may be a therapeutic solution. In this study, we divided BMSCs from T2DM rats (BMSCs-D) and normal rats (BMSCs-N), identified their ability to differentiate into different cell types...
May 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29772189/comparison-of-intraoral-bone-regeneration-with-iliac-and-alveolar-bmscs
#2
F Wang, Y Zhou, J Zhou, M Xu, W Zheng, W Huang, W Zhou, Y Shen, K Zhao, Y Wu, D Zou
This study compared the osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) of iliac and alveolar origins (I-BMSCs and Al-BMSCs, respectively), which were transplanted in combination with β tricalcium phosphate (β-TCP) in peri-implant bone defects to investigate the osseointegration between dental implants and tissue-engineered bone in dogs. Specifically, I-BMSCs and Al-BMSCs were cultured, characterized, and seeded on β-TCP and subjected to immunoblotting analyses and alkaline phosphatase activity assays...
May 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29767463/mechanical-and-biological-properties-of-a-biodegradable-mg-zn-ca-porous-alloy
#3
Yong-Qiang Zhang, Yang Li, Huan Liu, Jing Bai, Ni-Rong Bao, Yue Zhang, Peng He, Jian-Ning Zhao, Li Tao, Feng Xue, Guang-Xin Zhou, Gen-Tao Fan
OBJECTIVES: As promising alternative to current metallic biomaterials, the porous Mg scaffold with a 3-D open-pore framework has drawn much attention in recent years due to its suitable biodegradation, biocompatibility, and mechanical properties for human bones. This experiment's aim is to study the mechanical properties, biosafety, and osteogenesis of porous Mg-Zn alloy. METHODS: A porous Mg-2Zn-0.3Ca (wt%) alloy was successfully prepared by infiltration casting, and the size of NaCl particles was detected by a laser particle size analyzer...
May 16, 2018: Orthopaedic Surgery
https://www.readbyqxmd.com/read/29765422/the-effect-of-pulsatile-flow-on-bmsc-derived-endothelial-like-cells-in-a-small-sized-artificial-vessel-made-by-3-dimensional-bioprinting
#4
Kang Woog Lee, Dae-Hyun Kim, Jun Hee Lee, Young-Nam Youn
Replacement of small-sized vessels is still challenging. This study is aimed at investigating the possibility of small-sized artificial vessels made by 3-dimensional bioprinting and the effect of pulsatile flow on bMSC-derived endothelial-like cells. Cells were harvested from rabbit bone marrow and primary cultured with or without growth factors. Endothelial differentiation was confirmed by the Matrigel tube formation assay, Western blot, and qRT-PCR. In addition, embedment of endothelial-like cells in an artificial vessel was made by 3-dimensional bioprinting, and the pulsatile flow was performed...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29765415/human-recombinant-peptide-sponge-enables-novel-less-invasive-cell-therapy-for-ischemic-stroke
#5
Michiyuki Miyamoto, Kentaro Nakamura, Hideo Shichinohe, Tomohiro Yamauchi, Masaki Ito, Hisayasu Saito, Masahito Kawabori, Toshiya Osanai, Tasuku Sasaki, Kiyohiro Houkin, Satoshi Kuroda
Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29765408/the-interaction-between-bmal1-and-per2-in-mouse-bmsc-osteogenic-differentiation
#6
Haiya Zhuo, Yuhong Wang, Qing Zhao
The circadian clock is a system that controls endogenous time of organisms, and it regulates the physiology and behavior of bodies. The transcription factors Brain and Muscle ARNT-like Protein 1 (BMAL1) and Period2 (Per2) are components of the circadian clock, and they play vital roles in circadian clock function. Both Bmal1-/- mice and Per2-/- mice display obvious bone volume changes. In this study, we inhibited the expression of Bmal1 in bone marrow-derived mesenchymal stem cells (BMSCs) using a lentiviral vector harboring RNAi sequences, which increased the osteogenic differentiation capability of BMSCs...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29765406/the-differentiation-balance-of-bone-marrow-mesenchymal-stem-cells-is-crucial-to-hematopoiesis
#7
REVIEW
Jiang Wu, Weiwei Zhang, Qian Ran, Yang Xiang, Jiang F Zhong, Shengwen Calvin Li, Zhongjun Li
Bone marrow mesenchymal stem cells (BMSCs), the important component and regulator of bone marrow microenvironment, give rise to hematopoietic-supporting stromal cells and form hematopoietic niches for hematopoietic stem cells (HSCs). However, how BMSC differentiation affects hematopoiesis is poorly understood. In this review, we focus on the role of BMSC differentiation in hematopoiesis. We discussed the role of BMSCs and their progeny in hematopoiesis. We also examine the mechanisms that cause differentiation bias of BMSCs in stress conditions including aging, irradiation, and chemotherapy...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29765377/neutrophils-inhibit-synthesis-of-mineralized-extracellular-matrix-by-human-bone-marrow-derived-stromal-cells-in-vitro
#8
Okan W Bastian, Michiel Croes, Jacqueline Alblas, Leo Koenderman, Luke P H Leenen, Taco J Blokhuis
Although controlled local inflammation is essential for adequate bone regeneration, several studies have shown that hyper-inflammatory conditions after major trauma are associated with impaired fracture healing. These hyper-inflammatory conditions include the trauma-induced systemic inflammatory response to major injury, open fractures, and significant injury to the surrounding soft tissues. The current literature suggests that increased or prolonged influx of neutrophils into the fracture hematoma may mediate impairment of bone regeneration after hyper-inflammatory conditions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29763517/novel-engineered-tendon-fibrocartilage-bone-composite-with-cyclic-tension-for-rotator-cuff-repair
#9
Qian Liu, Taku Hatta, Jun Qi, Haoyu Liu, Andrew R Thoreson, Peter C Amadio, Steven L Moran, Scott P Steinmann, Anne Gingery, Chunfeng Zhao
Surgical repair of rotator cuff tears presents a significant clinical challenge with high failure rates and inferior functional outcomes. Graft augmentation improves repair outcomes, however currently available grafting materials have limitations. While cell-seeded decellularized tendon slices may facilitate cell infiltration, promote tendon incorporation and preserve original mechanical strength, the unique fibrocartilage zone is yet to be successfully reestablished. In this study, we investigated the biological and mechanical properties of an engineered tendon-fibrocartilage-bone composite (TFBC) with cyclic tension (3% strain, 0...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29757659/bone-marrow-derived-mesenchymal-stem-cells-promoted-cutaneous-wound-healing-by-regulating-keratinocyte-migration-via-the-%C3%AE-2-adrenergic-receptor-signaling
#10
Jiahui Huo, Sujing Sun, Zhijun Geng, Wei Sheng, Runkai Chen, Kui Ma, Xiaoyan Sun, Xiaobing Fu
Mesenchymal stem cells (MSCs) play an important role in the cutaneous wound healing; however, the functional mechanisms involved in the healing process are poorly understood. A series of studies indicate that keratinocytes which migrate into the wound bed rely on an epithelial-mesenchymal transition (EMT)-like process to initiate re-epithelialization. We therefore examined whether bone marrow-derived MSCs (BMSCs) could affect biological behavior and induce EMT-like characteristics in the human epidermal keratinocytes (HEKs) and in the immortalized human keratinocyte cell line HaCaT cells; and we investigated the signaling pathways of BMSC-mediated phenotypic changes...
May 14, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29749549/enhanced-proliferation-and-differentiation-of-ho-1-gene-modified-bone-marrow-derived-mesenchymal-stem-cells-in-the-acute-injured-kidney
#11
Nanmei Liu, Huiling Wang, Guofeng Han, Jin Cheng, Weifeng Hu, Jinyuan Zhang
The aim of the present study was to investigate the effect of heme oxygenase-1 (HO-1) overexpression on the survival and differentiation ability of bone marrow‑derived mesenchymal stem cells (BMSCs) in the acute kidney injury (AKI) microenvironment. HO-1-BMSCs and enhanced green fluorescent protein (eGFP)-BMSCs were constructed. Rat ischemia/reperfusion (I/R)‑AKI-kidney homogenate supernatant was prep-ared to treat the BMSCs, eGFP-BMSCs and HO-1-BMSCs in vitro. In the AKI microenvironment, the HO-1-BMSCs exhibited a smaller proportion of cells at the G0/G1 phase, and a larger proportion of cells expressing proliferating cell nuclear antigen (PCNA) and cytokeratin 18 (CK18)...
May 11, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29737241/human-mesenchymal-stromal-cell-sheets-induce-macrophages-predominantly-to-an-anti-inflammatory-phenotype
#12
Panithi Sukho, Jan Willem Hesselink, Nicole Kops, Jolle Kirpensteijn, Femke Verseijnden, Yvonne Bastiaansen-Jenniskens
Tissue healing is a highly complex process involving a cascade of biochemical and cellular events. Excessive inflammation can impair the healing response. Previous in vitro studies have shown that mesenchymal stromal cells can modulate macrophage-induced inflammation and therefore are promising candidates for cell-based therapies aimed at promoting tissue repair. Recently, cell sheets were introduced as a new method of delivering stromal cells to the repair-site. The goal of the current study was to compare the effect of different types of stromal cell sheets on the inflammatory state of macrophages <i>in vitro</i>...
May 8, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29736392/quercetin-stimulates-bone-marrow-mesenchymal-stem-cell-differentiation-through-an-estrogen-receptor-mediated-pathway
#13
Xin-Gang Pang, Yu Cong, Ni-Rong Bao, Yong-Gang Li, Jian-Ning Zhao
Objectives: The present study aimed to investigate the overall effect of quercetin on mouse bone marrow mesenchymal stem cell (BMSC) proliferation and osteogenic differentiation in vitro . Materials and Methods: BMSCs were treated with different concentrations of quercetin for 6 days. The effects of quercetin on cell proliferation were assessed at predetermined times using Cell Counting Kit-8 (CCK-8) assay. The cells were then treated with quercetin, estrogen, or an estrogen receptor (ER) antagonist (which was also administered in the presence of quercetin or estrogen) for 7 or 21 days...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29717383/-18-f-dpa-714-pet-imaging-shows-immunomodulatory-effect-of-intravenous-administration-of-bone-marrow-stromal-cells-after-transient-focal-ischemia
#14
Chengbo Tan, Songji Zhao, Kei Higashikawa, Zifeng Wang, Masahito Kawabori, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Naoyuki Ukon, Hironobu Yasui, Nagara Tamaki, Yuji Kuge, Hideo Shichinohe, Kiyohiro Houkin
BACKGROUND: The potential application of bone marrow stromal cell (BMSC) therapy in stroke has been anticipated due to its immunomodulatory effects. Recently, positron emission tomography (PET) with [18 F]DPA-714, a translocator protein (TSPO) ligand, has become available for use as a neural inflammatory indicator. We aimed to evaluate the effects of BMSC administration after transient middle cerebral artery occlusion (MCAO) using [18 F]DPA-714 PET. The BMSCs or vehicle were administered intravenously to rat MCAO models at 3 h after the insult...
May 2, 2018: EJNMMI Research
https://www.readbyqxmd.com/read/29717356/mesenchymal-stem-cells-seeded-onto-tissue-engineered-osteoinductive-scaffolds-enhance-the-healing-process-of-critical-sized-radial-bone-defects-in-rat
#15
Ahmad Oryan, Mohamadreza Baghaban Eslaminejad, Amir Kamali, Samaneh Hosseini, Ali Moshiri, Hossein Baharvand
Long bone defects comprise one of the most prevalent clinical problems worldwide and the current bone grafting materials have major limitations to repair them. Although tremendous efforts have been made to repair critical-sized long bone defects in animal models, designing an optimal bone tissue-engineered substitute remains one of the main challenges. Hence, this study aims to closely mimic a natural bone healing process by a tissue-engineered construct including osteoinductive materials pre-seeded with bone marrow-derived mesenchymal stem cells (BMSCs)...
May 1, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29716948/tension-loaded-bone-marrow-stromal-cells-potentiate-the-paracrine-osteogenic-signaling-of-co-cultured-vascular-endothelial-cells
#16
Yu Nan Jiang, Jun Zhao, Feng Ting Chu, Yang Yang Jiang, Guo Hua Tang
Co-culture of bone marrow stromal cells (BMSCs) and vascular endothelial cells (VECs) is a promising strategy for better osteogenesis and pre-vascularization in bone tissue engineering. Recent reports have shown that mechanical stretching further promotes osteogenesis in BMSC/VEC co-culture systems, but the underlying mechanism of this process remains unclear. In this study, noncontact co-cultures of rat primary BMSCs and VECs were employed to interrogate paracrine cell-to-cell communications in response to tensions...
May 1, 2018: Biology Open
https://www.readbyqxmd.com/read/29705655/spatially-confined-induction-of-endochondral-ossification-by-functionalized-hydrogels-for-ectopic-engineering-of-osteochondral-tissues
#17
Chiara Stüdle, Queralt Vallmajó Martín, Alexander Haumer, Julien Guerrero, Matteo Centola, Arne Mehrkens, Dirk J Schaefer, Martin Ehrbar, Andrea Barbero, Ivan Martin
Despite the various reported approaches to generate osteochondral composites by combination of different cell types and materials, engineering of templates with the capacity to autonomously and orderly develop into cartilage-bone bi-layered structures remains an open challenge. Here, we hypothesized that the embedding of cells inducible to endochondral ossification (i.e. bone marrow derived mesenchymal stromal cells, BMSCs) and of cells capable of robust and stable chondrogenesis (i.e. nasal chondrocytes, NCs) adjacent to each other in bi-layered hydrogels would develop directly in vivo into osteochondral tissues...
April 21, 2018: Biomaterials
https://www.readbyqxmd.com/read/29705604/bone-marrow-mesenchymal-stem-cells-conditioned-medium-protects-vsc4-1-cells-against-2-5-hexanedione-induced-autophagy-via-ngf-pi3k-akt-mtor-signaling-pathway
#18
Xin Zhang, Ying Kong, Yijie Sun, Zhiqiang Qian, Chenxue Gao, Xiaoxia Shi, Shuangyue Li, Yongjun Piao, Fengyuan Piao
We aimed to investigate the effects of bone marrow mesenchymal stem cell conditioned medium (BMSC-CM) in preventing 2,5-hexanedione (HD)-induced damage to motoneurons, and examined the molecular mechanisms that mediate these effects. VSC4.1 cells were exposed to 25 mM HD for 24 h followed by incubation with DMEM for 24 h. HD-treated cells were incubated with BMSC-CM at varied concentrations. Incubation with BMSC-CM ameliorated the decreased cell viability and reduced LDH release from cells exposed to HD. BMSC-CM suppressed the elevated number of autophagic vacuoles, cells with LC3 puncta, increased LC3-II/LC3-I ratio, and decreased p62 caused by HD exposure...
April 26, 2018: Brain Research
https://www.readbyqxmd.com/read/29702747/allogeneic-chondrogenically-differentiated-human-bone-marrow-stromal-cells-do-not-induce-dendritic-cell-maturation
#19
C H Kiernan, A KleinJan, M Peeters, E B Wolvius, E Farrell, P A J Brama
Bone marrow stromal cell (BMSC) mediated endochondral bone formation may be a promising alternative to the current gold standards of autologous bone transplantation, in the development of novel methods for bone repair. Implantation of chondrogenically differentiated BMSCs leads to bone formation in vivo via endochondral ossification. The success of this bone formation in an allogeneic system depends upon the interaction between the implanted constructs and the host immune system. The current study investigated the effect of chondrogenically differentiated hBMSC pellets on the maturation and function of dendritic cells (DCs) by directly co-culturing bone forming chondrogenic hBMSC pellets and immature or lipopolysaccharide (LPS)-matured DCs in vitro...
April 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29702290/construction-of-tendon-replacement-tissue-based-on-collagen-sponge-and-mesenchymal-stem-cells-by-coupled-mechano-chemical-induction-and-evaluation-of-its-tendon-repair-abilities
#20
Bingyu Zhang, Qing Luo, Bin Deng, Yasuyuki Morita, Yang Ju, Guanbin Song
Tissue engineering is an ideal therapeutic strategy for the development of functional tendon replacement tissue for tendon repair in the clinic. Currently, the synergistic roles of mechano-chemical factors and the mechanisms involved in tendon repair and regeneration are not fully understood. In this study, we developed a three-dimensional (3D) culture system based on a silicone chamber and collagen sponge scaffold that can deliver cyclic mechanical stretch and biochemical stimulation to bone marrow-derived mesenchymal stem cells (BMSCs) seeded on the scaffold...
April 24, 2018: Acta Biomaterialia
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