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https://www.readbyqxmd.com/read/28342879/stable-subcutaneous-cartilage-regeneration-of-bone-marrow-stromal-cells-directed-by-chondrocyte-sheet
#1
Dan Li, Lian Zhu, Yu Liu, Zongqi Yin, Yi Liu, Fangjun Liu, Aijuan He, Shaoqing Feng, Yixin Zhang, Zhiyong Zhang, Wenjie Zhang, Wei Liu, Yilin Cao, Guangdong Zhou
In vivo niche plays an important role in regulating differentiation fate of stem cells. Due to lack of proper chondrogenic niche, stable cartilage regeneration of bone marrow stromal cells (BMSCs) in subcutaneous environments is always a great challenge. This study explored the feasibility that chondrocyte sheet created chondrogenic niche retained chondrogenic phenotype of BMSC engineered cartilage (BEC) in subcutaneous environments. Porcine BMSCs were seeded into biodegradable scaffolds followed by 4 weeks of chondrogenic induction in vitro to form BEC, which were wrapped with chondrocyte sheets (Sheet group), acellular small intestinal submucosa (SIS, SIS group), or nothing (Blank group) respectively and then implanted subcutaneously into nude mice to trace the maintenance of chondrogenic phenotype...
March 22, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28339007/mir%C3%A2-217-inhibits-osteogenic-differentiation-of-rat-bone-marrow%C3%A2-derived-mesenchymal-stem-cells-by-binding-to-runx2
#2
Yu-Long Zhu, Shui Wang, De-Gang Ding, Liang Xu, Hai-Tao Zhu
The elucidation of the underlying molecular mechanisms regulating the osteogenic differentiation of bone marrow‑derived mesenchymal stem cells (BMSCs) is of great importance in improving the treatment of bone‑associated diseases. MicroRNAs (miRNAs) have been proven to regulate the osteogenic differentiation of BMSCs. The present study investigated the role of miR‑217 in the osteogenic differentiation of rat BMSCs. It was observed that miR‑217 expression levels were downregulated during the process of osteogenic differentiation...
March 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28338178/the-involvement-of-senescence-induced-by-the-telomere-shortness-in-the-decline-of-osteogenic-differentiation-in-bmscs
#3
C Li, G-J Wei, L Xu, J-S Rong, S-Q Tao, Y-S Wang
OBJECTIVE: BMSC (Bone marrow mesenchymal stem cells) is an important seed cell for the repair of bone and cartilage defect in the tissue engineering. The proliferation rate and differentiation capacity of BMSCs from the old donors were less than that from young donors; however, the related mechanism remained unclear. MATERIALS AND METHODS: Sprague Dawley (SD) rats BMSCs were cultured and treated. Hydrogen peroxide (H2O2) and continual passage of BMSCs were performed to induce senescence...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28320453/dysregulated-systemic-lymphocytes-affect-the-balance-of-osteogenic-adipogenic-differentiation-of-bone-mesenchymal-stem-cells-after-local-irradiation
#4
Xiaoya Xu, Ruixia Li, Yi Zhou, Qiong Zou, Qiaoling Ding, Jinfeng Wang, Weifang Jin, Guoqiang Hua, Jianjun Gao
BACKGROUND: While it is known that irradiation can induce local and systemic bone loss over time, how focal irradiation induces systemic bone complications remains unclear. Immune cells are thought to be crucial to bone homeostasis, and abnormal immune cells lead to serious disruption of bone homeostasis, such as in acute lymphoblastic leukaemia. This disruption primarily occurs due to inhibition of the osteogenic differentiation of bone mesenchymal stem cells (BMSCs). METHODS: In this study, we detected local and systemic bone loss in trabecular bone by micro-computed tomography (micro-CT) and measurement of peroxisome proliferator-activated receptor gamma (PPARγ) and runt-related transcription factor 2 (RUNX2) expression in BMSCs using real-time polymerase chain reaction and western blotting...
March 20, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28319735/different-combinations-of-growth-factors-for-the-tenogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-in-monolayer-culture-and-in-fibrin-based-three-dimensional-constructs
#5
Marta Bottagisio, Silvia Lopa, Valentina Granata, Giuseppe Talò, Chiara Bazzocchi, Matteo Moretti, Arianna Barbara Lovati
Tendon injuries are severe burdens in clinics. The poor tendon healing is related to an ineffective response of resident cells and inadequate vascularization. Thanks to the high proliferation and multi-lineage differentiation capability, bone marrow-derived mesenchymal stem cells (BMSCs) are a promising cell source to support the tendon repair. To date, the association of various growth factors to induce the in vitro tenogenic differentiation of multipotent progenitor cells is poorly investigated. This study aimed to investigate the tenogenic differentiation of rabbit BMSCs by testing the combination of bone morphogenetic proteins (BMP-12 and 14) with transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) both in 2D and 3D cultures within fibrin-based constructs...
March 16, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/28318921/influence-of-tgf-%C3%AE-1-on-tumor-transition-in-oral-cancer-cell-and-bmsc-co-cultures
#6
F Böhrnsen, F Godek, J Kiesel, F J Kramer, P Brockmeyer, H Schliephake
OBJECTIVES: TGF-β1 signaling modulates epithelial mesenchymal transitions (EMT) of head and neck squamous cell carcinoma (HNSCC). Bone marrow mesenchymal stromal cells (BMSC) are able to exert a regulating influence on the expression of markers of EMT in HNSCC cells. It was thus the aim of this study to test the hypothesis that TGF-β1 modulates the interactions of tumor transition between BMSCs and HNSCC, affecting the expression of E-cadherin, Vimentin, Snail, Twist, MMP14 and beta-catenin...
February 17, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28316120/synergistic-effects-of-g-csf-and-bone-marrow-stromal-cells-on-nerve-regeneration-with-acellular-nerve-xenografts
#7
Hua Jia, Ying Wang, Tao Wang, Yi Dong, Wei-Li Li, Jun-Ping Li, Wen-Zhi Ma, Xiao-Jie Tong, Zhong-Yi He
Peripheral nerve defects result in severe denervation presenting sensory and motor functional incapacitation. Currently, a satisfactory therapeutic treatment promoting the repair of injured nerves is not available. As shown in our previous study, acellular nerve xenografts (ANX) implanted with bone marrow stromal cells (BMSCs) replaced allografts and promoted nerve regeneration. Additionally, granulocyte-colony stimulating factor (G-CSF) has been proven to mobilize supplemental cells and enhance vascularization in the niche...
March 18, 2017: Synapse
https://www.readbyqxmd.com/read/28314478/osteoinductive-effects-of-glyceollins-on-adult-mesenchymal-stromal-stem-cells-from-adipose-tissue-and-bone-marrow
#8
Marjorie E Bateman, Amy L Strong, Ryan S Hunter, Melyssa R Bratton, Rajesh Komati, Jayalakshmi Sridhar, Kevin E Riley, Guangdi Wang, Daniel J Hayes, Stephen M Boue, Matthew E Burow, Bruce A Bunnell
BACKGROUND: While current therapies for osteoporosis focus on reducing bone resorption, the development of therapies to regenerate bone may also be beneficial. Promising anabolic therapy candidates include phytoestrogens, such as daidzein, which effectively induce osteogenesis of adipose-derived stromal cells (ASCs) and bone marrow stromal cells (BMSCs). PURPOSE: To investigate the effects of glyceollins, structural derivatives of daidzein, on osteogenesis of ASCs and BMSCs...
April 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28301671/a-clinical-study-of-bone-mesenchymal-stem-cells-for-the-treatment-of-hepatic-fibrosis-induced-by-hepatolenticular-degeneration
#9
D Zhang
The efficacy of bone marrow mesenchymal stem cell (BMSC) on liver fibrosis in animal has been proven, but a few studies have been made in human body and few such researches in China. This study was designed to investigate the effect of BMSC treatment on hepatic fibrosis induced by hepatolenticular degeneration and the influence on serological indicators. Sixty patients with liver fibrosis induced by hepatolenticular degeneration were randomly divided into two groups, a penicillamine group and a BMSCs plus penicillamine group, with 30 patients in each...
March 15, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28300491/dynamic-regulation-of-twist1-expression-during-chondrogenic-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells
#10
Mairéad A Cleary, Roberto Narcisi, Anna Albiero, Florien Jenner, Laurie M G de Kroon, Wendy J L M Koevoet, Pieter A J Brama, Gerjo J V M van Osch
Human bone marrow-derived mesenchymal stem cells (BMSCs) are clinically promising to repair damaged articular cartilage. This study investigated TWIST1, an important transcriptional regulator in mesenchymal lineages, in BMSC chondrogenesis. We hypothesized that downregulation of TWIST1 expression is required for in vitro chondrogenic differentiation. Indeed, significant downregulation of TWIST1 was observed in murine skeletal progenitor cells during limb development (N = 3 embryos), and during chondrogenic differentiation of culture-expanded human articular chondrocytes (N = 3 donors) and isolated adult human BMSCs (N = 7 donors), consistent with an inhibitory effect of TWIST1 expression on chondrogenic differentiation...
March 16, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28292847/human-umbilical-cord-blood-borne-fibroblasts-contain-marrow-niche-precursors-that-form-a-bone-marrow-organoid-in-vivo
#11
Alice Pievani, Benedetto Sacchetti, Alessandro Corsi, Benedetta Rambaldi, Samantha Donsante, Valeria Scagliotti, Patrizia Vergani, Cristina Remoli, Andrea Biondi, Pamela G Robey, Mara Riminucci, Marta Serafini
Human umbilical cord blood (CB) has attracted much attention as a reservoir for functional hematopoietic stem and progenitor cells, and, recently, as a source of blood-borne fibroblasts (CB-BFs). Previously, we demonstrated that bone marrow stromal cell (BMSC) and CB-BF pellet cultures make cartilage in vitro Furthermore, upon in vivo transplantation, BMSC pellets remodelled into miniature bone/marrow organoids. Using this in vivo model, we asked whether CB-BF populations that express characteristics of the hematopoietic stem cell (HSC) niche contain precursors that reform the niche...
March 15, 2017: Development
https://www.readbyqxmd.com/read/28282033/pi3k%C3%AE-and-pi3k%C3%AE-isoforms-have-distinct-functions-in-regulating-pro-tumoural-signalling-in-the-multiple-myeloma-microenvironment
#12
R E Piddock, N Loughran, C R Marlein, S D Robinson, D R Edwards, S Yu, G E Pillinger, Z Zhou, L Zaitseva, M J Auger, S A Rushworth, K M Bowles
Phosphoinositide-3-kinase and protein kinase B (PI3K-AKT) is upregulated in multiple myeloma (MM). Using a combination of short hairpin RNA (shRNA) lentivirus-mediated knockdown and pharmacologic isoform-specific inhibition we investigated the role of the PI3K p110γ (PI3Kγ) subunit in regulating MM proliferation and bone marrow microenvironment-induced MM interactions. We compared this with inhibition of the PI3K p110δ (PI3kδ) subunit and with combined PI3kδ/γ dual inhibition. We found that MM cell adhesion and migration were PI3Kγ-specific functions, with PI3kδ inhibition having no effect in MM adhesion or migration assays...
March 10, 2017: Blood Cancer Journal
https://www.readbyqxmd.com/read/28267421/use-of-pig-as-a-model-for-mesenchymal-stem-cell-therapies-for-bone-regeneration
#13
Marcello Rubessa, Kathryn Polkoff, Massimo Bionaz, Elisa Monaco, Derek J Milner, Scott J Holllister, Michael S Goldwasser, Matthew B Wheeler
Bone is a plastic tissue with a large healing capability. However, extensive bone loss due to disease or trauma requires extreme therapy such as bone grafting or tissue-engineering applications. Presently, bone grafting is the gold standard for bone repair, but presents serious limitations including donor site morbidity, rejection, and limited tissue regeneration. The use of stem cells appears to be a means to overcome such limitations. Bone marrow mesenchymal stem cells (BMSC) have been the choice thus far for stem cell therapy for bone regeneration...
March 7, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28260319/-protective-effect-and-related-mechanism-of-tanshinol-borneol-ester-on-homocysteine-induced-rat-bone-marrow-mesenchymal-stem-cells-damage
#14
X Wang, J Yang, H Yuan, X L Liu, Y Li, P Jia, X H Zheng, X P Zheng
Objective: To investigate the protective effect and potential mechanism of tanshinol borneol ester (TBE) on homocysteine(Hcy) induced rat bone marrow mesenchymal stem cells (BMSCs) damage. Methods: BMSCs were isolated and cultured in vitro by density gradient centrifugation and adherent culture method. BMSCs were divided into the control (normal isolation and culture), TBE-1(10 μmol/L TBE-1 solution with 100 μl), TBE-2 (10 μmol/L TBE-2 solution with 100 μl), Hcy (0.5 mmol/L Hcy solution with 100 μl), Hcy + TBE-1(0...
February 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28260000/intraparenchymal-treatment-with-bone-marrow-mesenchymal-stem-cell-conditioned-medium-exerts-neuroprotection-following-intracerebral-hemorrhage
#15
Changmeng Cui, Ying Cui, Junling Gao, Ran Li, Xiaohua Jiang, Yanxia Tian, Kaijie Wang, Jianzhong Cui
Intracerebral hemorrhage (ICH) is a life-threatening type of stroke. Previous studies have reported that bone marrow mesenchymal stem cells (BMSCs) may exert beneficial effects on the treatment of ICH. However, it remains unknown whether the neuroprotection exerted by BMSCs on ICH is due to the differentiation of BMSCs, or the trophic factors secreted into their conditioned medium (CM). In addition, growth‑associated protein‑43 (GAP‑43) is a protein associated with neurite extension, which may be considered a prospective therapeutic target in the treatment of ICH...
February 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28246930/association-of-plasma-sdf-1-with-bone-mineral-density-body-composition-and-hip-fractures-in-older-adults-the-cardiovascular-health-study
#16
Laura D Carbone, Petra Bůžková, Howard A Fink, John A Robbins, Monique Bethel, Mark W Hamrick, William D Hill
Aging is associated with an increase in circulating inflammatory factors. One, the cytokine stromal cell-derived factor 1 (SDF-1 or CXCL12), is critical to stem cell mobilization, migration, and homing as well as to bone marrow stem cell (BMSC), osteoblast, and osteoclast function. SDF-1 has pleiotropic roles in bone formation and BMSC differentiation into osteoblasts/osteocytes, and in osteoprogenitor cell survival. The objective of this study was to examine the association of plasma SDF-1 in participants in the cardiovascular health study (CHS) with bone mineral density (BMD), body composition, and incident hip fractures...
February 28, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28243602/enhancement-of-bone-marrow-derived-mesenchymal-stem-cell-angiogenic-capacity-by-npwt-for-a-combinatorial-therapy-to-promote-wound-healing-with-large-defect
#17
Kangquan Shou, Yahui Niu, Xun Zheng, Zhanjun Ma, Chao Jian, Baiwen Qi, Xiang Hu, Aixi Yu
Poor viability of engrafted bone marrow mesenchymal stem cells (BMSCs) often hinders their application for wound healing, and the strategy of how to take full advantage of their angiogenic capacity within wounds still remains unclear. Negative pressure wound therapy (NPWT) has been demonstrated to be effective for enhancing wound healing, especially for the promotion of angiogenesis within wounds. Here we utilized combinatory strategy using the transplantation of BMSCs and NPWT to investigate whether this combinatory therapy could accelerate angiogenesis in wounds...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28218578/bone-marrow-derived-mesenchymal-stem-cells-ameliorate-nephrosis-through-repair-of-impaired-podocytes
#18
Yi Chen, Jun Chen, Jianxin Wan, Na Gao, Jiong Cui, Danyu You, Zhenhuan Zou
PURPOSE: The purpose of this study was to investigate the effects of bone marrow-derived mesenchymal stem cells (BMSC) on podocytes of puromycin amino nuclear glucoside (PAN) -induced nephrosis in mice. METHODS: Mice were randomly divided into Control, PAN and BMSC groups. Mice were injected with PAN (0.5 mg/g weight) via the tail vein. The 24-h urinary protein was obtained after modelling, and urinary protein excretion was determined. The blood and kidney specimens were isolated after the tenth day of modelling...
February 19, 2017: Clinical and Investigative Medicine. Médecine Clinique et Experimentale
https://www.readbyqxmd.com/read/28216148/epigenetic-modification-of-the-ccl5-ccr1-erk-axis-enhances-glioma-targeting-in-dedifferentiation-reprogrammed-bmscs
#19
Rui Chen, Wayne Yuk-Wai Lee, Xiao Hu Zhang, Jie Ting Zhang, Sien Lin, Liang Liang Xu, Biao Huang, Fu Yuan Yang, Hai Long Liu, Bin Wang, Lai Ling Tsang, Sandrine Willaime-Morawek, Gang Li, Hsiao Chang Chan, Xiaohua Jiang
The success of stem cell-mediated gene therapy in cancer treatment largely depends on the specific homing ability of stem cells. We have previously demonstrated that after in vitro induction of neuronal differentiation and dedifferentiation, bone marrow stromal cells (BMSCs) revert to a primitive stem cell population (De-neu-BMSCs) distinct from naive BMSCs. We report here that De-neu-BMSCs express significantly higher levels of chemokines, and display enhanced homing abilities to glioma, the effect of which is mediated by the activated CCL5/CCR1/ERK axis...
March 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28214894/mechanical-loading-improves-tendon-bone-healing-in-a-rabbit-anterior-cruciate-ligament-reconstruction-model-by-promoting-proliferation-and-matrix-formation-of-mesenchymal-stem-cells-and-tendon-cells
#20
Fanglong Song, Dawei Jiang, Tianchen Wang, Yi Wang, Fengmei Chen, Guoxing Xu, Yifan Kang, Yinquan Zhang
BACKGROUND/AIMS: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). METHODS: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface...
February 16, 2017: Cellular Physiology and Biochemistry
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