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https://www.readbyqxmd.com/read/28804540/restoration-of-bone-defects-using-modified-heterogeneous-deproteinized-bone-seeded-with-bone-marrow-mesenchymal-stem-cells
#1
Jun Li, Zeyu Huang, Liyan Chen, Xin Tang, Yue Fang, Lei Liu
The aim of the present study was to investigate the effect of modified heterogeneous deproteinized bone combined with bone marrow mesenchymal stem cells (BMSCs) in the restoration of a validated bone defect model. BMSCs were identified by flow cytometry and multilineage differentiation assay. The structural features of the modified heterogeneous deproteinized bone scaffold and biocompatibility between BMSCs and the scaffold were confirmed by scanning electron microscope (SEM) detection. The cytotoxicity of the modified heterogeneous deproteinized bone scaffolds were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28777945/long-term-engraftment-of-primary-bone-marrow-stromal-cells-repairs-niche-damage-and-improves-hematopoietic-stem-cell-transplantation
#2
Jean-Paul Abbuehl, Zuzana Tatarova, Werner Held, Joerg Huelsken
Hematopoietic stem cell (HSC) transplantation represents a curative treatment for various hematological disorders. However, delayed reconstitution of innate and adaptive immunity often causes fatal complications. HSC maintenance and lineage differentiation are supported by stromal niches, and we now find that bone marrow stroma cells (BMSCs) are severely and permanently damaged by the pre-conditioning irradiation required for efficient HSC transplantation. Using mouse models, we show that stromal insufficiency limits the number of donor-derived HSCs and B lymphopoiesis...
August 3, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28769765/mesenchymal-stem-cell-derived-extracellular-vesicles-ameliorates-hippocampal-synaptic-impairment-after-transient-global-ischemia
#3
Mingyang Deng, Han Xiao, Hainan Zhang, Hongling Peng, Huan Yuan, Yunxiao Xu, Guangsen Zhang, Zhiping Hu
Recent studies have found that administration of stem cells or extracellular vehicles (EVs) derived from stem cells exert neuroprotective effects after transient global ischemia. However, the underlying mechanisms of this effect remain unclear, especially at the level of synaptic functions. In this study, we compared the suppressive effects on cyclooxygenase-2 (COX-2) upregulation by EVs derived from bone marrow mesenchymal stem cells (BMSC-EV), adipose tissue MSC (AdMSC-EV) and serum (serum-EV). Then we examined whether BMSC-EVs could restore functional integrity of synaptic transmission and plasticity...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28739546/in-situ-tissue-regeneration-through-sdf-1%C3%AE-driven-cell-recruitment-and-stiffness-mediated-bone-regeneration-in-a-critical-sized-segmental-femoral-defect
#4
Amaia Cipitria, Kathrin Boettcher, Sophia Schoenhals, Daniela S Garske, Katharina Schmidt-Bleek, Agnes Ellinghaus, Anke Dienelt, Anja Peters, Manav Mehta, Christopher M Madl, Nathaniel Huebsch, David J Mooney, Georg N Duda
In-situ tissue regeneration aims to utilize the body's endogenous healing capacity through the recruitment of host stem or progenitor cells to an injury site. Stromal cell-derived factor-1 α (SDF-1 α) is widely discussed as a potent chemoattractant. Here we use a cell-free biomaterial-based approach to (i) deliver SDF-1 α for the recruitment of endogenous bone marrow-derived stromal cells (BMSC) into a critical-sized segmental femoral defect in rats and to (ii) induce hydrogel stiffness-mediated osteogenic differentiation in-vivo...
July 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28733324/nadph-oxidase-2-derived-superoxide-drives-mitochondrial-transfer-from-bone-marrow-stromal-cells-to-leukemic-blasts
#5
Christopher R Marlein, Lyubov Zaitseva, Rachel E Piddock, Stephen Robinson, Dylan Edwards, Manar S Shafat, Zhigang Zhou, Matthew Lawes, Kristian M Bowles, Stuart A Rushworth
Improvements in the understanding of the metabolic cross-talk between cancer and its micro-environment are expected to lead to novel therapeutic approaches. Acute myeloid leukemia (AML) cells have increased mitochondria compared to non-malignant CD34+ hematopoietic progenitor cells. Furthermore, contrary to the Warburg hypothesis, (AML) relies on oxidative phosphorylation to generate ATP. Here we report that in human AML, NOX2 generates superoxide which stimulates bone marrow stromal cells (BMSC) to AML blast transfer of mitochondria through AML derived tunnelling nanotubes...
July 21, 2017: Blood
https://www.readbyqxmd.com/read/28714177/curved-microstructures-promote-osteogenesis-of-mesenchymal-stem-cells-via-the-rhoa-rock-pathway
#6
Qi Zhang, Shiyu Lin, Tao Zhang, Taoran Tian, Quanquan Ma, Xueping Xie, Changyue Xue, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: Cells in the osteon reside in a curved space, accordingly, the curvature of the microenvironment is an important geometric feature in bone formation. However, it is not clear how curved microstructures affect cellular behaviour in bone tissue. MATERIALS AND METHODS: Rat primary bone marrow mesenchymal stem cells (BMSCs) on wavy microgrooves were exposed to PDMS substrates with various curvatures to investigate alterations in cellular morphology and osteogenic differentiation...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28713978/bone-marrow-mesenchymal-stem-cell%C3%A2-derived-extracellular-vesicles-improve-the-survival-of-transplanted-fat-grafts
#7
He Huang, Shaoqing Feng, Wenjie Zhang, Wei Li, Peng Xu, Xiangsheng Wang, Ai Ai
Autologous fat grafting is a promising surgical technique for soft tissue augmentation, reconstruction and rejuvenation. However, it is limited by the low survival rate of the transplanted fat, due to the slow revascularization of such grafts. Previous studies have demonstrated that bone marrow mesenchymal stem cell‑derived extracellular vesicles (BMSC‑EVs) are proangiogenic. The present study aimed to investigate whether BMSC‑EVs could improve the survival of transplanted fat grafts. Extracellular vesicles were isolated from the supernatant of cultured rat bone marrow mesenchymal stem cells, and characterized by flow cytometry and scanning electron microscopy...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713741/reconstruction-of-drug-induced-cleft-palate-using-bone-marrow-mesenchymal-stem-cell-in-rodents
#8
Julie Christy Amalraj, Manasa Gangothri, Hari Babu
BACKGROUND: Triamcinolone acetonide (TAC) (Kenacort*) is a commonly used synthetic glucocorticoid in today's medical practice. The drug is also a potential agent in inducing cleft palates in rats. This drug has been used to induce cleft palate in the fetus of the pregnant rats to bring out a suitable animal model for human cleft lip and palate. The drug was given intraperitoneally to induce congenital cleft palate in pregnant mother rats. AIM: The aim of this study is to induce congenital cleft palate in pregnant Wister albino rats and reconstruct the defect with bone marrow mesenchymal stem cells (BMSCs) isolated from the same species along with PLGA (poly lactic co glycolic acid) scaffold...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28701922/bone-marrow-mesenchymal-stem-cell-transplantation-promotes-functional-improvement-associated-with-cntf-stat3-activation-after-hemi-sectioned-spinal-cord-injury-in-tree-shrews
#9
Liu-Lin Xiong, Fei Liu, Bing-Tuan Lu, Wen-Ling Zhao, Xiu-Juan Dong, Jia Liu, Rong-Ping Zhang, Piao Zhang, Ting-Hua Wang
Hemi-sectioned spinal cord injury (hSCI) can lead to spastic paralysis on the injured side, as well as flaccid paralysis on the contralateral side, which can negatively affect a patient's daily life. Stem-cell therapy may offer an effective treatment option for individuals with hSCI. To examine the role of bone marrow mesenchymal stem cells (BMSCs) transplantation on hSCI and explore related mechanisms in the tree shrews, here, we created a model of hSCI by inducing injury at the tenth thoracic vertebra (T10)...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28691752/mineral-trioxide-aggregate-improves-healing-response-of-periodontal-tissue-to-injury-in-mice
#10
I Vidovic Zdrilic, I O de Azevedo Queiroz, B G Matthews, J E Gomes-Filho, M Mina, I Kalajzic
BACKGROUND AND OBJECTIVE: Mineral trioxide aggregate (MTA) is a biomaterial used in endodontic procedures as it exerts beneficial effects on regenerative processes. In this study, we evaluate the effect of MTA on healing of periodontal ligament (PDL) and surrounding tissue, following injury, in a transgenic mouse model and on the differentiation of murine mesenchymal progenitor cells in vitro. MATERIAL AND METHODS: We used an inducible Cre-loxP in vivo fate mapping approach to examine the effects of MTA on the contributions of descendants of cells expressing the αSMA-CreERT2 transgene (SMA9(+) ) to the PDL and alveolar bone after experimental injury to the root furcation on the maxillary first molars...
July 10, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28681586/effects-on-cytotoxicity-and-antibacterial-properties-of-the-incorporations-of-silver-nanoparticles-into-the-surface-coating-of-dental-alloys
#11
Xiao-Ting Shen, Yan-Zhen Zhang, Fang Xiao, Jing Zhu, Xiao-Dong Zheng
The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO3+NaBH4→ Ag+1/2H2+1/2B2H6+NaNO3. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28679425/overexpression-of-c-met-in-bone-marrow-mesenchymal-stem-cells-improves-their-effectiveness-in-homing-and-repair-of-acute-liver-failure
#12
Kun Wang, Yuwen Li, Tiantian Zhu, Yongting Zhang, Wenting Li, Wenyu Lin, Jun Li, Chuanlong Zhu
BACKGROUND: Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) has emerged as a novel therapy for acute liver failure (ALF). However, the homing efficiency of BMSCs to the injured liver sites appears to be poor. In this study, we aimed to determine if overexpression of c-Met in BMSCs could promote the homing ability of BMSCs to rat livers affected by ALF. METHODS: Overexpression of c-Met in BMSCs (c-Met-BMSCs) was attained by transfection of naive BMSCs with the lenti-c-Met-GFP...
July 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28675146/evaluation-of-the-effectiveness-of-a-bmsc-and-bmp-2-polymeric-trilayer-system-in-cartilage-repair
#13
Raquel Vayas, Ricardo Reyes, María Rodríguez-Évora, Carlos Del Rosario, Araceli Delgado, Carmen Évora
In this study a poly(lactide-co-glycolide) acid (PLGA) tri-layer scaffold is proposed for cartilage repair. The trilayer system consists of a base layer formed by a tablet of PLGA microspheres, a second layer composed of a microsphere suspension placed on top of the tablet, and the third layer, which constitutes an external electrospun PLGA thin polymeric membrane. Combinations of bone morphogenetic protein-2 (BMP-2) encapsulated in the microspheres of the suspension layer, and bone marrow mesenchymal stem cells (bMSC) seeded on the electrospun membrane, are evaluated by histologic analyses and immunohistochemistry in a critical size osteochondral defect in rabbits...
July 4, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28675022/-tumor-necrosis-factor-%C3%AE-regulates-the-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-in-chronic-periodontitis
#14
Li Xiaoguang, Wang Yizhu, Guo Bin
Bone marrow mesenchymal stem cells (BMSCs) and ideal adult stem cells for alveolar bone regeneration considerably help restore the structure and function of the periodontium and promote the healing of periodontal disease. Thus, BMSC features, especially the mechanism of osteogenic differentiation, has recently become a research hotspot. Tumor necrosis factor-α (TNF-α), which is the main factor in the periodontal inflammatory microenvironment, is directly related to the osteogenic differentiation of BMSCs...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28672913/amniotic-membrane-derived-stem-cells-help-repair-osteochondral-defect-in-a-weight-bearing-area-in-rabbits
#15
Zhijin Zhang, Linru Zeng, Jun Yang, Lin Guo, Qiao Hou, Fangbing Zhu
Our study evaluated the use of amniotic membrane-derived stem cells for repairing osteochondral defects in a weight-bearing area in rabbits. Twenty-four 3-month-old male or female New Zealand white rabbits were selected. The rabbits were randomly divided into 3 groups of eight, according to the treatment received for an experimentally inflicted femoral medial malleolus lesion, group I received a human acellular amniotic membrane seeded with bone marrow-derived mesenchymal stem cells (HAAM-BMSCs) implant; group II received a simple HAAM implant and the control group received no experimental lesion or treatment...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28667462/the-evaluation-of-cartilage-differentiations-using-transforming-growth-factor-beta3-alone-and-with-combination-of-bone-morphogenetic-protein-6-on-adult-stem-cells
#16
C C Ude, H C Chen, M Y Norhamdan, B M Azizi, B S Aminuddin, B H I Ruszymah
In our quest to standardize our formula for a clinical trial, transforming growth factor-beta3 (TGF-β3) alone and in combination with bone morphogenetic protein-6 (BMP-6) were evaluated for their effectiveness in cartilage differentiation. Bone Marrow Stem Cells (BMSCs) and Adipose Derived Stem Cells (ADSCs) were induced to chondrogenic lineage using two different media. Native chondrocytes served as positive control. ADSCs and BMSCs proved multipotency by tri-lineage differentiations. ADSC has significantly higher growth kinetics compare to Chondrocyte only p ≤ 0...
June 30, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28659496/secretion-of-interleukin-6-by-bone-marrow-mesenchymal-stem-cells-promotes-metastasis-in-hepatocellular-carcinoma
#17
Fei Mi, Liansheng Gong
Mesenchymal stem cells (MSCs) interact with tumor cells and regulate tumorigenesis and metastasis. As one of the important components of the tumor microenvironment, MSC-secreted cytokines play a critical role in cancer development. However, whether and how bone marrow MSCs (BMSCs) and their secreted cytokines participate in hepatocellular carcinoma (HCC) progression, still remains largely unknown. In the present study, we first measured the concentration of interleukin-6 (IL-6) in BMSC conditioned medium (BMSC-CM)...
June 28, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28656221/effect-of-transplantation-of-olfactory-ensheathing-cell-conditioned-medium-induced-bone-marrow-stromal-cells-on-rats-with-spinal-cord-injury
#18
Linjie Feng, Hongquan Gan, Wenguo Zhao, Yingjie Liu
Spinal cord injury is a serious threat to human health and various techniques have been deployed to ameliorate or cure its effects. Stem cells transplantation is one of the promising methods. The primary aim of the present study was to investigate the effect of the transplantation of olfactory ensheathing cell (OEC) conditioned medium‑induced bone marrow stromal cells (BMSCs) on spinal cord injury. Rat spinal cord compression injury animal models were generated, and the rats divided into the following three groups: Group A, (control) Dulbecco's modified Eagle's medium‑treated group; group B, normal BMSC‑treated group; group C, OEC conditioned medium‑induced BMSC‑treated group...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28649933/bone-mesenchymal-stem-cell-conditioned-medium-decreases-the-generation-of-astrocytes-during-the-process-of-neural-stem-cells-differentiation
#19
Huang Fang, Peiwen Song, Yuening Shen, Cailiang Shen, Xiaoying Liu
OBJECTIVES: The aim of this study was to investigate the effect of bone mesenchymal stem cell (BMSC) conditioned medium (CM) and Bone morphogenetic protein-4 (BMP-4) on the generation of astrocytes during the process of NSCs differentiation. DESIGN: Neural stem cells (NSCs) were grown under different culture conditions. SETTING: The First Affiliated Hospital of Anhui Medical University, Hefei, China. OUTCOME MEASURES: The study consisted of four groups: NSCs cultured under control conditions (group 1) or with the addition of BMSC-CM (group 2);(BMP-4) (group 3) or both (group 4)...
June 26, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/28642327/declining-histone-acetyltransferase-gcn5-represses-bmsc-mediated-angiogenesis-during-osteoporosis
#20
Huan Jing, Li Liao, Xiaoxia Su, Yi Shuai, Xinjing Zhang, Zhihong Deng, Yan Jin
Angiogenesis is disrupted in age-related and postmenopausal osteoporosis. However, the mechanisms of the disorder remain elusive. We confirmed in this study that, in accordance with the decrease of H-type vessels, the proangiogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) declined during osteoporosis. Screening of the histone acetyltransferase family revealed that GCN5 decreased in BMSCs derived from osteoporotic femur. Further analysis identified that GCN5 plays important roles in regulating the proangiogenic potential of BMSCs...
June 22, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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