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https://www.readbyqxmd.com/read/29349973/-progress-on-surgical-treatment-for-femoral-head-preservering-in-the-precollapse-stage-of-femoral-head-necrosis
#1
REVIEW
Yi-Heng Ye, Kai Chen, Ke-Ke Jin, Yi-Fan Zhang, Lei Chen
Osteonecrosis of the femoral head(ONFH), a refractory disease characterized by death of the osteocytes and the bone marrow due to inadequate blood supply caused by various mechanisms, usually leads to the collapse of the femoral head and malfunction of the hip joint. The crux is to diagnose ONFH early in the precollapse stage and prevent subsequent progression of collapsing through early interventions, thus delaying or avoiding the replacement of the hip joint. A number of joint salvaging operation treatments for early stage ONFH are available...
March 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29337251/chromatin-organization-regulated-by-ezh2-mediated-h3k27me3-is-required-for-opn-induced-migration-of-bone-marrow-derived-mesenchymal-stem-cells
#2
Lingling Liu, Qing Luo, Jinghui Sun, Yang Ju, Yasuyuki Morita, Guanbin Song
Osteopontin (OPN) is a chemokine-like extracellular matrix-associated protein involved in the migration of bone marrow-derived mesenchymal stem cells (BMSCs). An increasing number of studies have found that chromatin organization may affect cellular migration. However, whether OPN regulates chromatin organization is not understood, nor are the underlying molecular mechanisms. In this study, we investigated the link between chromatin organization and BMSC migration and demonstrated that OPN-mediated BMSC migration leads to elevated levels of heterochromatin marker histone H3 lysine 27 trimethylation (H3K27me3) through the methyltransferase EZH2...
January 11, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29332347/genetically-engineered-bone-marrow-derived-mesenchymal-stem-cells-co-expressing-ifn-%C3%AE-and-il-10-inhibit-hepatocellular-carcinoma-by-modulating-mapk-pathway
#3
Hao Wang, Jun Wang, Xiaolei Shi, Yitao Ding
PURPOSE: One of the major challenges in delivering cytokines for the treatment of hepatocellular carcinoma (HCC) is the mode of delivery. This study hypothesized that genetically engineered bone marrow derived mesenchymal stem cells (BMSCs) co-expressing IFN-γ and IL-10 can serve as a potential therapeutic strategy in the treatment of HCC by inhibiting cell proliferation. METHODS: Male Sprague-Dawley rats (n=5, 200-250 g) for BMSCs isolation and Nude/SCID mice (n=35,12-20g) to develop liver cancer xenograft model were used...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29331291/global-microrna-profiling-in-human-bone-marrow-skeletal-stromal-or-mesenchymal-stem-cells-identified-candidates-for-bone-regeneration
#4
Chi-Chih Chang, Morten T Venø, Li Chen, Nicholas Ditzel, Dang Q S Le, Philipp Dillschneider, Moustapha Kassem, Jørgen Kjems
Bone remodeling and regeneration are highly regulated multistep processes involving posttranscriptional regulation by microRNAs (miRNAs). Here, we performed a global profiling of differentially expressed miRNAs in bone-marrow-derived skeletal cells (BMSCs; also known as stromal or mesenchymal stem cells) during in vitro osteoblast differentiation. We functionally validated the regulatory effects of several miRNAs on osteoblast differentiation and identified 15 miRNAs, most significantly miR-222 and miR-423, as regulators of osteoblastogenesis...
December 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29296725/replenishing-exosomes-from-older-bone-marrow-stromal-cells-with-mir-340-inhibits-myeloma-related-angiogenesis
#5
Tomohiro Umezu, Satoshi Imanishi, Kenko Azuma, Chiaki Kobayashi, Seiichiro Yoshizawa, Kazuma Ohyashiki, Junko H Ohyashiki
The study of bone marrow stromal cells (BMSCs) and the exosomes they secrete is considered promising for cancer therapy. However, little is known about the effect of donor age on BMSCs. In the present study, we investigated the therapeutic potential of BMSC exosomes derived from donors of different ages using an in vivo model of hypoxic bone marrow in multiple myeloma (MM). We found that donor age was strongly related to senescent changes in BMSCs. Exosomes derived from young BMSCs significantly inhibited MM-induced angiogenesis in Matrigel plugs...
May 23, 2017: Blood Advances
https://www.readbyqxmd.com/read/29290626/tet2-loss-dysregulates-the-behavior-of-bone-marrow-mesenchymal-stromal-cells-and-accelerates-tet2-driven-myeloid-malignancy-progression
#6
Rong Li, Yuan Zhou, Zeng Cao, Lin Liu, Jinhuan Wang, Zizhen Chen, Wen Xing, Shi Chen, Jie Bai, Weiping Yuan, Tao Cheng, Mingjiang Xu, Feng-Chun Yang, Zhigang Zhao
TET2 is a methylcytosine dioxygenase that regulates cytosine hydroxymethylation. Although there are extensive data implicating a pivotal role of TET2 in hematopoietic stem/progenitor cells (HSPCs), the importance of TET2 in bone marrow mesenchymal stromal cells (BMSCs) remains unknown. In this study, we show that loss of TET2 in BMSCs increases cell proliferation and self-renewal and enhances osteoblast differentiation potential of BMSCs, which may in turn alter their behavior in supporting HSPC proliferation and differentiation...
January 9, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29279573/effect-of-local-bone-marrow-stromal-cell-administration-on-ligature-induced-periodontitis-in-mice
#7
Shinya Iguchi, Daigo Suzuki, Eisuke Kawano, Takayuki Mashimo, Mikihito Kajiya, Taku Toriumi, Toshihisa Kawai, Hidemi Kurihara, Keitaro Isokawa, Shuichi Sato, Masaki Honda
Bone marrow-derived multipotent stromal cells (BMSCs) have potent antiinflammatory effects. This study aimed to investigate the antiinflammatory potential of BMSCs using a mouse model of ligature-induced periodontitis. BMSCs were isolated from the femurs and tibiae of mice. Periodontitis was induced by placing a ligature around the right maxillary second molar. After 3 days, the mice were administered BMSC in the gingiva of the mesial interdental papilla around the ligatured molar. The ligatured and non-ligatured mice that were not administered BMSC served as controls...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29274877/comparisons-of-the-therapeutic-effects-of-three-different-routes-of-bone-marrow-mesenchymal-stem-cell-transplantation-in-cerebral-ischemic-rats
#8
Hong-Lian Zhang, Xu-Fang Xie, Ying-Qiong Xiong, Shi-Min Liu, Guo-Zhu Hu, Wen-Feng Cao, Xiao-Mu Wu
Bone marrow mesenchymal stem cells (BMSCs) are mainly administered via three routes: intra-arterial, intravenous and intracerebral. It has been reported that BMSC administration via each route ameliorates the functional deficits after cerebral ischemia. However, there have been no comparisons of the therapeutic benefits of BMSC administration through different delivery routes. In this study, we injected BMSCs into a rat model of transient middle cerebral artery occlusion (MCAO) through the intra-arterial, intravenous, or intracerebral route at day 7 after MCAO...
December 21, 2017: Brain Research
https://www.readbyqxmd.com/read/29274438/the-treatment-of-spinal-cord-injury-in-rats-using-bone-marrow-derived-neural-like-cells-induced-by-cerebrospinal-fluid
#9
Ying Ye, Ting-Ting Feng, Yi-Ran Peng, Shu-Qun Hu, Tie Xu
This study aimed to evaluate the effect of bone mesenchymal stem cells (BMSCs) and BMSC neural-like cells (BMSC-Ns) on the spinal cord injury (SCI) in the rat model of SCI. BMSC-Ns were prepared from the third passage of BMSCs by induction of healthy cerebrospinal fluid (CSF) of an adult human. The SCI rat model was established through a surgical procedure, and after 7 days the rats were randomly divided into 3 (A, B and C) groups. Groups A (BMSC-Ns) and B (BMSCs) were treated with 1 × 106/20 μl cells, while group C (saline) was treated with saline, all via intracerebroventricular injection...
December 20, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29274248/stem-cell-education-for-medical-students-at-tongji-university-primary-cell-culture-and-directional-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells
#10
Caixia Jin, Haibin Tian, Jiao Li, Song Jia, Siguang Li, Guo-Tong Xu, Lei Xu, Lixia Lu
Stem cells are cells that can self-renew and differentiate into a variety of cell types under certain conditions. Stem cells have great potential in regenerative medicine and cell therapy for the treatment of certain diseases. To deliver knowledge about this frontier in science and technology to medical undergraduate students, we designed an innovative practical experiment for freshmen in their second semester. The lab exercise focused on rat bone marrow mesenchymal stem cell (BMSC) isolation, cell culture and differentiation, and it aimed to help students master the aseptic techniques for cell culture, the basic methods and procedures for the primary culture and passage of BMSCs, the basic procedure for the directional differentiation of BMSCs into adipocytes and their subsequent identification by oil-red-O staining...
December 23, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/29258846/bone-marrow-mesenchymal-stromal-cells-alleviate-brain-white-matter-injury-via-the-enhanced-proliferation-of-oligodendrocyte-progenitor-cells-in-focal-cerebral-ischemic-rats
#11
Xiaohe Yu, Hongjuan Wu, Yansong Zhao, Yuanyuan Guo, Yuxi Chen, Peng Dong, Qingjie Mu, Xin Wang, Xiaoli Wang
The effects of transplanting bone marrow mesenchymal stromal cells (BMSCs) for the treatment of white matter damage are not well understood, nor are the underlying mechanisms. Recent studies showed that endogenous oligodendrocyte progenitor cells (OPCs)can be stimulated to proliferate. Therefore, we explore the effects of BMSCs transplantation on white matter damage and the proliferation of OPCs in transient focal cerebral ischemic rats.BMSCs were transplanted into a group of rats that had undergone middle cerebral artery occlusion(MCAO) 24 hours after reperfusion...
December 16, 2017: Brain Research
https://www.readbyqxmd.com/read/29250137/enhanced-osseointegration-of-titanium-implants-in-a-rat-model-of-osteoporosis-using-multilayer-bone-mesenchymal-stem-cell-sheets
#12
Yan Duan, Wei Ma, Dehua Li, Tongfei Wang, Baolin Liu
The present study aimed to investigate whether bone marrow-derived mesenchymal stem cell (BMSC) sheets combined with titanium implants enhanced implant osseointegration in an ovariectomized (OVX) rat model of osteoporosis. Sprague-Dawley rats were randomly assigned into a test group and control group. Allogenic BMSCs were collected from the rats, cultured and stored via cryopreservation. At 6 months post-ovariectomy, establishment of the OVX model was confirmed by micro-computed tomography (CT) measurements...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29242228/bmscs-ameliorate-septic-coagulopathy-through-suppressing-inflammation-in-cecal-ligation-and-puncture-induced-sepsis
#13
Shunyao Xu, Zhen Zhou, Hao Li, Ziying Liu, Xiaojun Pan, Fen Wang, Yueyue Huang, Xiaogang Li, Yunbei Xiao, Jingye Pan, Cong Wang, Dequan Li
Sepsis is an aggressive and life-threatening systemic inflammatory response with a high mortality. Inflammation and coagulation play crucial roles in the pathogenesis of sepsis in a mutually promoting manner. Unlike other single-target molecular therapies that have no obvious effects on clinical sepsis, the bone marrow stromal cell (BMSC) therapy offers a broader spectrum of activities ranging from immune and inflammation suppression to tissue regeneration. In this report, we demonstrated that BMSC injection attenuated the septic coagulopathy...
December 14, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29239597/degradation-of-bioresorbable-mg-4zn-1sr-intramedullary-pins-and-associated-biological-responses-in-vitro-and-in-vivo
#14
Aaron F Cipriano, Jiajia Lin, Alan Lin, Amy Sallee, Belinda Le, Mayra Celene Cortez Alcaraz, Ren-Guo Guan, Gary Botimer, Serkan Inceoğlu, Huinan Liu
This article reports the degradation and biological properties of as-drawn Mg-4Zn-1Sr (designated as ZSr41) and pure Mg (P-Mg) wires as bioresorbable intramedullary pins for bone repair. Specifically, their cytocompatibility with bone marrow derived mesenchymal stem cells (BMSCs) and degradation in vitro, and their biological effects on peri-implant tissues and in vivo degradation in rat tibiae were studied. The as-drawn ZSr41 pins showed a significantly faster degradation than P-Mg in vitro and in vivo. The in vivo average daily degradation rates of both ZSr41 and P-Mg intramedullary pins were significantly greater than their respective in vitro degradation rates, likely because the intramedullary site of implantation is highly vascularized for removal of degradation products...
December 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29237155/paracrine-activation-of-the-wnt-%C3%AE-catenin-pathway-by-bone-marrow-stem-cell-attenuates-cisplatin-induced-kidney-injury
#15
Xiaoyan Jiao, Jieru Cai, Xiaofang Yu, Xiaoqiang Ding
BACKGROUND/AIMS: Cisplatin-induced acute kidney injury (AKI) involves damage to tubular cells via excess reactive oxygen species (ROS) generation. Stem cell-based therapies have shown great promise in AKI treatment. In this study, we aimed to assess the protective effect and mechanism of bone marrow mesenchymal stem cell (BMSC)-derived conditioned medium (CM) against cisplatin-induced AKI. METHODS: In vitro, NRK-52E cells were incubated with cisplatin in the presence or absence of CM, followed by the assessment of cell viability, apoptosis and cell cycle distribution...
December 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29234953/involvement-of-microrna-23b-in-tnf-%C3%AE-reduced-bmsc-osteogenic-differentiation-via-targeting-runx2
#16
Lin Deng, Guoli Hu, Lei Jin, Chenglong Wang, Hongwen Niu
Elucidation of the molecular mechanism governing bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation is of great importance for improving the treatment of osteoporosis. TNF-α is a well-known inhibitory factor during osteogenic differentiation of BMSCs. In our experiment, we consistently observed that TNF-α significantly inhibited BMSC osteogenic differentiation, which was partially rescued by BAY 11-7082 (NF-κB inhibitor). In this study, we examined the potential roles of microRNAs (miRNAs) involved in TNF-α-mediated reduction of BMSC osteogenesis...
December 12, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29228886/continuous-stimulation-with-differentiation-factors-is-necessary-to-enhance-osteogenic-differentiation-of-human-mesenchymal-stem-cells-in-vitro
#17
Bruno Reible, Gerhard Schmidmaier, Matthäus Prokscha, Arash Moghaddam, Fabian Westhauser
Bone defect treatment belongs to the most challenging fields in orthopedic surgery and requires the well-coordinated application of mesenchymal stem cells (MSC) and differentiation factors. MSC isolated from reaming material (RMSC) and iliac crest (BMSC) in combination with bone morphogenetic protein-7 (BMP-7) and insulin-like growth factor-1 (IGF-1) have been used. The short half-life of both factors limit their applications: a burst release of the factor can probably not induce sustainable differentiation...
October 2017: Growth Factors
https://www.readbyqxmd.com/read/29228730/wisp2-disruption-represses-cxcr4-expression-and-inhibits-bmscs-homing-to-injured-liver
#18
Dan Qin, Yi Yan, Bian Hu, Wanpo Zhang, Hanmin Li, Xiaodong Li, Shenghui Liu, Depeng Dai, Xiongji Hu, Xingxu Huang, Lisheng Zhang
Liver regeneration/repair is a compensatory regrowth following acute liver failure, and bone marrow-derived mesenchyme stem cell (BMSC) transplantation is an effective therapy that promotes liver regeneration/repair. Wnt1 inducible signaling pathway protein 2 (Wisp2) is highly expressed in BMSCs, however, its function remains unclear. In this work, we used clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein -9 nuclease (CRISPR/Cas9) genome editing technology to knockdown Wisp2 in BMSCs, and these modified cells were then transplanted into rats which were induced by the 2-AAF/PH...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29216639/exendin-4-induces-bone-marrow-stromal-cells-migration-through-bone-marrow-derived-macrophages-polarization-via-pka-stat3-signaling-pathway
#19
Ning Wang, Jian Gao, Min Jia, Xue Ma, Zhanxiang Lei, Fei Da, Fei Yan, Huinan Zhang, Ying Zhou, Mingkai Li, Gonghao He, Jingru Meng, Xiaoxing Luo
BACKGROUND/AIMS: The synthesis and degradation processes involved in bone remodeling are critically regulated by osteoblasts and osteoclasts. The GLP-1 receptor agonist Exendin-4 is beneficial for osteoblast differentiation and increases the number of osteoblasts. METHODS: We constructed an ovariectomized model to evaluate the impact of Exendin-4 on bone formation in osteoporosis. A macrophage-depleted model was also created to investigate the effect of macrophages on bone formation...
December 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#20
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
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