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https://www.readbyqxmd.com/read/28524176/lentivirus-mediated-microrna-124-gene-modified-bone-marrow-mesenchymal-stem-cell-transplantation-promotes-the-repair-of-spinal-cord-injury-in-rats
#1
Jia-Lin Song, Wei Zheng, Wei Chen, Yun Qian, Yuan-Ming Ouyang, Cun-Yi Fan
Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group...
May 19, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28523456/bone-marrow-mesenchymal-stem-cell-administration-significantly-improves-outcome-after-retinal-ischemia-in-rats
#2
Biji Mathew, Jacqueline N Poston, John C Dreixler, Leianne Torres, Jasmine Lopez, Ruth Zelkha, Irina Balyasnikova, Maciej S Lesniak, Steven Roth
PURPOSE: Ischemia-associated retinal degeneration is one of the leading causes of vision loss, and to date, there are no effective treatment options. We hypothesized that delayed injection of bone-marrow stem cells (BMSCs) 24 h after the onset of ischemia could effectively rescue ischemic retina from its consequences, including apoptosis, inflammation, and increased vascular permeability, thereby preventing retinal cell loss. METHODS: Retinal ischemia was induced in adult Wistar rats by increasing intraocular pressure (IOP) to 130-135 mmHg for 55 min...
May 18, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28514770/mm-bmscs-induce-na%C3%A3-ve-cd4-t-lymphocytes-dysfunction-through-fibroblast-activation-protein-%C3%AE
#3
Xiaofei Wu, Yadan Wang, Jian Xu, Ting Luo, Jun Deng, Yu Hu
BACKGROUND: The tumor microenvironment plays a major role in multiple myelomas (MM). MM-BMSCs (bone marrow mesenchymal stromal cells) can support tumor growth and immune surveillance escape. On the other hand, fibroblast activation protein α, expressed by cancer stroma cells including BMSCs, has been shown to potentiate epithelial cancers growth and immune suppression. RESULTS: MM-BMSC inhibited proliferation of T cells (P = 0.0138), promoted senescence of T cells (P < 0...
April 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28505619/bone-marrow-mesenchymal-stem-cell-transplantation-increases-gap-43-expression-via-erk1-2-and-pi3k-akt-pathways-in-intracerebral-hemorrhage
#4
Jianzhong Cui, Changmeng Cui, Ying Cui, Ran Li, Huaxin Sheng, Xiaohua Jiang, Yanxia Tian, Kaijie Wang, Junling Gao
BACKGROUND/AIMS: Intracerebral hemorrhage (ICH) occurs in hypertensive patients and results in high rates of mortality and disability. This study determined whether bone marrow mesenchymal stem cell (BMSC) transplantation affects axonal regeneration and examined the underlying mechanisms after the administration of PD98059 (p-ERK1/2 inhibitor) or/ and LY294002 (PI3K inhibitor). The hypothesis that was intended to be tested was that BMSC transplantation regulates the expression of growth-associated protein-43 (GAP-43) via the ERK1/2 and PI3K/Akt signaling pathways...
May 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28502304/-inula-britannica-flower-total-flavonoids-reduces-the-apoptosis-of-aging-bone-marrow-mesenchymal-stem-cells-by-anti-oxidation
#5
Yuanyuan Long, Hui Chen, Lu Liu, Lei Guo
Objective To investigate the beneficial effect of Inula Britannica flower total flavonoids (IBFTF) on aging bone mesenchymal stem cell (BMSC) and its potential mechanism. Methods The aging BMSCs were induced by D-galactose, and then treated with 12.5, 25, 50 μg/mL IBFTF. The cell viability was detected by CCK-8 assay. The activity of catalase (CAT) and superoxide dismutase (SOD), the content of malondialdehyde (MDA) and reactive oxygen species (ROS) were measured by a commercial kit. The apoptosis was assessed by flow cytometry...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28492559/mk2206-enhances-the-cytocidal-effects-of-bufalin-in-multiple-myeloma-by-inhibiting-the-akt-mtor-pathway
#6
Ru-Fang Xiang, Yan Wang, Nan Zhang, Wen-Bin Xu, Yang Cao, Jia Tong, Jun-Min Li, Ying-Li Wu, Hua Yan
Despite the development of promising cancer therapeutic drugs, multiple myeloma (MM) remains an incurable disease. Bufalin is a bufanolide steroid compound of the traditional Chinese medicine Chan Su that was previously shown to exert growth suppression effects on myeloma cell lines. Previous studies conducted by our group demonstrated that bufalin activated the AKT/mTOR pathway in myeloma cells, which is considered an essential pathway to disease progression and is related to drug resistance in MM. In view of the significant role of AKT in MM, the allosteric AKT inhibitor MK2206 was selected in order to enhance the antitumor effects of bufalin in different MM cell lines (NCI-H929, U266, LP-1 and RPMI8226)...
May 11, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28491279/-mesenchymal-stem-cells-fact-or-fiction-and-implications-in-their-therapeutic-use
#7
REVIEW
Pamela Robey
The concept of a post-natal "mesenchymal stem cell" ("MSC") originated from studies focused on bone marrow stromal cells (BMSCs), which are non-hematopoietic adherent cells, a subset of which are skeletal stem cells (SSCs), able to form cartilage, bone, hematopoiesis-supportive stroma, and marrow adipocytes based on rigorous clonal and differentiation assays. Subsequently, it was speculated that BMSCs could form other mesodermal derivatives and even cell types from other germ layers. Based on BMSC surface markers, representative of fibroblastic cells, and imprecise differentiation assays, it was further imagined that "MSCs" are ubiquitous and equipotent...
2017: F1000Research
https://www.readbyqxmd.com/read/28478702/sdf-1-accelerates-cartilage-defect-repairing-by-recruiting-bmscs-and-promoting-chondrolgenic-differentiation
#8
Yuze Wang, Xiaojuan Sun, Jia Lv, Lingyuan Zeng, Xiaochun Wei, Lei Wei
Chemokine stromal cell-derived factor-1 (SDF-1) is a powerful chemoattractant for the localization of CXCR4 positive bone marrow stem cell (BMSC) into the bone marrow. We studied the effects of SDF-1 on the cartilage defect repair by recruiting BMSCs and promoting its chondrolgenic differentiation in vitro and in vivo. Chemotaxis analysis with Transwell plate showed that SDF-1 could recruit BMSCs through SDF-1/CXCR4 axis. RT-PCR, ELISA, and western blot results suggested that the levels of type II collagen and GAG were increased after incubation the BMSCs with SDF-1 compared with without SDF-1 group...
May 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28469796/low-dose-radiation-modulates-human-mesenchymal-stem-cell-proliferation-through-regulating-cdk-and-rb
#9
Lei Yang, Ziling Liu, Chen Chen, Xiaofeng Cong, Zhi Li, Shasha Zhao, Meng Ren
Low-dose radiation (LDR) has been known to stimulate cell proliferation. The effect of LDR on human bone marrow mesenchymal stem cells (BMSCs), however, remains to be determined. The current study, therefore, aimed to investigate the effect of LDR on human BMSC proliferation and its mechanisms. To accomplish this, human BMSCs were isolated from ribs and cultured with or without exposition to LDR (75 mGy) for 24 h. Cell proliferation was assessed by MTT assay, the cytokines secreted by the BMSCs were quantified by ELISA, and the proteins associated with cell proliferation and cell cycle were evaluated by immunoblot analysis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28462906/biomimetic-design-and-fabrication-of-multilayered-osteochondral-scaffolds-by-low-temperature-deposition-manufacturing-and-thermal-induced-phase-separation-techniques
#10
Ting Zhang, Hefeng Zhang, Laquan Zhang, Shuaijun Jia, Jian Liu, Zhuo Xiong, Wei Sun
Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it is necessary that scaffolds effectively mimic the extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic osteochondral scaffolds containing an oriented cartilage layer, a compact layer, and a three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The oriented cartilage layer was designed to mimic the structural and material characteristics of native cartilage tissue and was fabricated with cartilage matrix-chitosan materials, using thermal-induced phase-separation technology...
May 2, 2017: Biofabrication
https://www.readbyqxmd.com/read/28454328/reconstruction-of-mandibular-defects-with-autogenous-bone-and-decellularized-bovine-bone-grafts-with-freeze-dried-bone-marrow-stem-cell-paracrine-factors
#11
Ann Kakabadze, Konstantine Mardaleishvili, George Loladze, Lia Karalashvili, Gocha Chutkerashvili, David Chakhunashvili, Zurab Kakabadze
The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze-dried bone marrow stem cells (BMSCs) paracrine factors and three-dimensional bone scaffold derived from cancellous bovine bone following decellularization...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453953/dual-functioning-peptides-discovered-by-phage-display-increase-the-magnitude-and-specificity-of-bmsc-attachment-to-mineralized-biomaterials
#12
Harsha Ramaraju, Sharon J Miller, David H Kohn
Design of biomaterials for cell-based therapies requires presentation of specific physical and chemical cues to cells, analogous to cues provided by native extracellular matrices (ECM). We previously identified a peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) using phage display. The aims of this study were to identify a human MSC-specific peptide sequence through phage display, combine it with the apatite-specific sequence, and verify the specificity of the combined dual-functioning peptide to both apatite and human bone marrow stromal cells...
April 18, 2017: Biomaterials
https://www.readbyqxmd.com/read/28447748/tenascin-c-expression-and-its-associated-pathway-in-bmscs-following-co-culture-with-mechanically-stretched-ligament-fibroblasts
#13
Bing Zhao, Mengcai Hu, Huiyan Wu, Chenchen Ren, Jianshe Wang, Shihong Cui
The occurrence of pelvic organ prolapse (POP) is closely associated with alterations in the extracellular matrix proteins of the supporting ligament. Bone marrow mesenchymal stem cells (BMSCs) have the potential to differentiate into a variety of cell types, including osteoblasts, chondroblasts and adipocytes. Therefore, BMSCs have the potential to improve the clinical outcomes of POP. Tenascin‑C is a large glycoprotein that is present in the ECM and is involved in morphogenetic movements, and tissue patterning and repair...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28447618/manufacturing-differences-affect-human-bone-marrow-stromal-cell-characteristics-and-function-comparison-of-production-methods-and-products-from-multiple-centers
#14
Shutong Liu, Luis F de Castro, Ping Jin, Sara Civini, Jiaqiang Ren, Jo-Anna Reems, Jose Cancelas, Ramesh Nayak, Georgina Shaw, Timothy O'Brien, David H McKenna, Myriam Armant, Leslie Silberstein, Adrian P Gee, Derek J Hei, Peiman Hematti, Sergei A Kuznetsov, Pamela G Robey, David F Stroncek
Human bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) are manufactured using many different methods, but little is known about the spectrum of manufacturing methods used and their effects on BMSC characteristics and function. Seven centers using, and one developing, Good Manufacturing Practices (GMP) processes were surveyed as to their production methods. Among the seven centers, all used marrow aspirates as the starting material, but no two centers used the same manufacturing methods...
April 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28440742/leptin-overexpression-in-bone-marrow-stromal-cells-promotes-periodontal-regeneration-in-a-rat-model-of-osteoporosis
#15
Baoyu Zheng, Jun Jiang, Yuling Chen, Minkui Lin, Zhibin Du, Yin Xiao, Kai Luo, Fuhua Yan
BACKGROUND: Osteoporosis (OP) is associated with widespread periodontitis and impaired periodontal healing. However, there is a lack of information about the outcomes of regenerative approaches under the influence of OP. This study investigated the effect of leptin (LEP) overexpression on the regenerative potential of bone marrow stromal cells (BMSCs) in an osteoporotic rat periodontal fenestration defect model. METHODS: Rat BMSCs were transfected with adenoviruses harboring the human (h) LEP gene...
April 25, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28433514/cox-2-inhibition-potentiates-mouse-bone-marrow-stem-cell-engraftment-and-differentiation-mediated-wound-repair
#16
Ramasatyaveni Geesala, Neha R Dhoke, Amitava Das
BACKGROUND: Engraftment of transplanted stem cells is often limited by cytokine and noncytokine proinflammatory mediators at the injury site. We examined the role of Cyclooxygenase-2 (Cox-2)-induced cytokine-mediated inflammation on engraftment of transplanted bone marrow stem cells (BMSCs) at the wound site. METHODS: BMSCs isolated from male C57/BL6J mice were transplanted onto excisional splinting wounds in syngenic females in presence or absence of celecoxib, Cox-2 specific inhibitor (50 mg/kg, body weight [b wt]), to evaluate engraftment and wound closure...
April 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28420460/-bone-marrow-mesenchymal-stem-cells-modulated-the-inflammatory-response-by-regulating-the-expression-of-il-4-and-rage-products-in-the-rats-with-mods
#17
Xia Zhou, Guanghui Xiu, Yichao Zhu, Xiaolei Chen, Wei Xiong, Xinghua Pan, Jie Sun, Bin Ling
OBJECTIVE: To investigate the underlying mechanism of bone marrow mesenchymal stem cells (BMSC) modulating the inflammatory response during the multiple organ dysfunction syndrome (MODS), especially the expression of inflammatory cytokines, which will provide new theoretical and experimental basis of MODS in clinic. METHODS: BMSC of Sprague-Dawley (SD) rat (female, 4 weeks) was extracted and cultivated, and the 4th passage were used in experimental study. According to the random number table, 60 female SD rats were divided into three groups (n = 20 per group): sham group, MODS group, BMSC group...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28418864/bone-marrow-derived-mesenchymal-stem-cells-overexpressing-mir-21-efficiently-repair-myocardial-damage-in-rats
#18
Yan-Ling Zeng, Hao Zheng, Qiu-Ru Chen, Xiao-Hong Yuan, Jin-Hua Ren, Xiao-Feng Luo, Ping Chen, Zhe-Yao Lin, Shao-Zhen Chen, Xue-Qiong Wu, Min Xiao, Yong-Quan Chen, Zhi-Zhe Chen, Jian-Da Hu, Ting Yang
OBJECTIVE: We investigated the ability of bone marrow derived mesenchymal stem cells (BMSCs) overexpressing microRNA-21 (miR-21) to repair cardiac damage induced by anthracyclines in rats. METHODS: Sprague-Dawley (SD) rats of 2~3 weeks old were selected to isolate and culture BMSCs. A lentivirus harboring pLVX-miR-21 was generated and transfected into rat BMSCs. The rats were assigned into an untreated negative control group, and groups injected with adriamycin alone or with adriamycin followed by BMSCs, pLVX-BMSCs or pLVX-miR-21-BMSCs (n = 10 each)...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415701/wogonin-induces-retinal-neuron-like-differentiation-of-bone-marrow-stem-cells-by-inhibiting-notch-1-signaling
#19
Qinmeng Shu, Hong Zhuang, Jiawen Fan, Xin Wang, Gezhi Xu
Age-related macular degeneration and retinitis pigmentosa are major causes of irreversible vision loss in the elderly and, despite sustained efforts, current treatments are largely ineffective. Wogonin is a bioactive plant flavonoid possessing a range of beneficial properties, including neuroprotective effects. We investigated the ability of wogonin to promote retinal neuron-like differentiation of bone marrow stem cells (BMSCs) and assessed the involvement of Notch-1 signaling in this process. Cultured mouse BMSCs were left untreated or exposed to neurotrophic factors in the presence or absence of wogonin, and western blotting, RT-PCR and immunofluorescence were used to identify changes in molecular markers of stemness and neuroretinal differentiation...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28408732/glial-cell-line-derived-neurotrophic-factor-transfected-placenta-derived-versus-bone-marrow-derived-mesenchymal-cells-for-treating-spinal-cord-injury
#20
Yao Lu, Hui Gao, Man Zhang, Bing Chen, Huilin Yang
BACKGROUND Placenta-derived mesenchymal stem cells (PMSCs) were isolated from placenta and had differentiation and self-renewal potential. We transfected PMSCs with glial cell line-derived neurotrophic factor (GDNF) and compared their effect for repairing spinal cord injury (SCI) with that of GDNF-transfected bone marrow-derived mesenchymal stem cell (BMSC). MATERIAL AND METHODS The PMSCs were isolated from Sprague-Dawley rat placenta; BMSCs were isolated from Sprague-Dawley rat thigh bone marrow. Primary cultured BMSCs and PMSCs were uniformly spindle-shaped...
April 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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