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leukocyte esterase

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https://www.readbyqxmd.com/read/29679431/synthesis-and-characterization-of-pyridine-compounds-for-amperometric-measurements-of-leukocyte-esterase
#1
Douglas Hanson, Travis Menard, Teresa Blazek, Stanton McHardy, Waldemar Gorski
The present work introduces a new class of substrates for enzyme leukocyte esterase (LE). The proposed substrates (compounds I-III) react with LE yielding anodic current in a direct proportion to LE activity. The kinetic constants Km and kcat for the enzymatic reactions were determined by amperometry at a glassy carbon electrode. The binding affinity of compounds I-III for LE was two orders of magnitude better than that of existing optical LE substrates. The specificity constant kcat/Km was equal to 2.7, 3...
April 20, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29656969/the-leukocyte-esterase-test-strip-is-a-poor-rule-out-test-for-periprosthetic-joint-infection
#2
Carl A Deirmengian, Lihua Liang, John P Rosenberger, Tony R Joaquim, Martin R Gould, Patrick A Citrano, Keith W Kardos
BACKGROUND: The urinary leukocyte esterase (LE) test strip has been suggested as a good screening test for periprosthetic joint infection (PJI). The purpose of this study is to compare the diagnostic profile of LE assays from different manufacturers and determine whether the LE test strip is a good rule-out test. METHODS: Synovial fluid samples (N = 344), sent to 1 laboratory for PJI testing, were used in this prospective study. Four different tests for synovial fluid LE were simultaneously evaluated for their performance in detecting white blood cell (WBC) positive samples (>3000 cells/µL)...
March 13, 2018: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29618582/uropathogens-and-pyuria-in-children-with-neurogenic-bladders
#3
Catherine S Forster, Nader Shaikh, Alejandro Hoberman, Elizabeth Jackson
BACKGROUND: A recent study revealed that specific uropathogens are associated with lower odds of pyuria in a general pediatrics population. Children with neurogenic bladders who require clean intermittent catheterization (CIC) frequently have pyuria. Our objective with this study was to determine if an association exists between pyuria and type of uropathogen in CIC-dependent children. METHODS: We obtained urinalysis and urine culture results from electronic medical records from January 2008 through December 2014 for patients ≤18 years of age with neurogenic bladders managed at a single institution...
April 4, 2018: Pediatrics
https://www.readbyqxmd.com/read/29557491/a-meta-analysis-of-synovial-biomarkers-in-periprosthetic-joint-infection-synovasure%C3%A2-is-less-effective-than-the-elisa-based-alpha-defensin-test
#4
REVIEW
Sufian S Ahmad, Michael T Hirschmann, Roland Becker, Ahmed Shaker, Atesch Ateschrang, Marius J B Keel, Christoph E Albers, Lukas Buetikofer, Sithombo Maqungo, Ulrich Stöckle, Sandro Kohl
PURPOSE: (1) To determine the overall accuracy of synovial alpha-defensin, synovial C-reactive protein (sCRP), interleukin-6 (sIL-6), and leukocyte esterase (sLE) as diagnostic markers for periprosthetic joint infection (PJI) and (2) to independantly evaluate the accuracy of both the laboratory-based ELISA alpha-defensin test and the Synovasure™ alpha-defensin test kit. METHODS: An EMBASE and MEDLINE (PubMed) database search was performed using a set of professionally set search terms...
March 20, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29551303/the-2018-definition-of-periprosthetic-hip-and-knee-infection-an-evidence-based-and-validated-criteria
#5
Javad Parvizi, Timothy L Tan, Karan Goswami, Carlos Higuera, Craig Della Valle, Antonia F Chen, Noam Shohat
BACKGROUND: The introduction of the Musculoskeletal Infection Society (MSIS) criteria for periprosthetic joint infection (PJI) in 2011 resulted in improvements in diagnostic confidence and research collaboration. The emergence of new diagnostic tests and the lessons we have learned from the past 7 years using the MSIS definition, prompted us to develop an evidence-based and validated updated version of the criteria. METHODS: This multi-institutional study of patients undergoing revision total joint arthroplasty was conducted at 3 academic centers...
February 26, 2018: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29479748/ascorbic-acid-a-black-hole-of-urine-chemistry-screening
#6
Adriana Unic, Nora Nikolac Gabaj, Marijana Miler, Jelena Culej, Adrijana Lisac, Anita Horvat, Nada Vrkic
BACKGROUND: Study was performed in order: (i) to assess the comparability of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; (ii) to assess accuracy of glucose, bilirubin, hemoglobin, leukocyte esterase, and protein; and (iii) to evaluate interference of ascorbic acid on the glucose, bilirubin, hemoglobin, and nitrite determination using 2 different dipsticks: iChem Velocity, Iris Diagnostics and Combur-10M, Roche Diagnostics. METHODS: Random urine specimens were included in the study...
February 25, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29470628/bladder-debris-on-ultrasound-in-the-emergency-department-correlation-with-urinalysis
#7
Ghaneh Fananapazir, Behrad Golshani, Ling-Xin Chen, John P McGahan, Angelo M de Mattos, Michael T Corwin
PURPOSE: To evaluate the correlation between the presence of bladder debris on ultrasound and urinalysis results in the emergency department setting. METHODS: Adult patients presenting to the emergency department with an ultrasound of the bladder and a urinalysis performed within 24 h of the ultrasound were included in this retrospective study. Two radiologists in consensus evaluated for the presence or absence of debris within the bladder. Urinalysis results were recorded including continuous variables (specific gravity and pH) and categorical variables (presence of occult blood, bilirubin, ketones, glucose, protein, urobilinogen, nitrite, leukocyte esterase, white blood cells, and red blood cells)...
February 22, 2018: Abdominal Radiology
https://www.readbyqxmd.com/read/29427551/quantitative-urine-test-strip-reading-for-leukocyte-esterase-and-hemoglobin-peroxidase
#8
Matthijs N Oyaert, Jonas Himpe, Marijn M Speeckaert, Veronique V Stove, Joris R Delanghe
BACKGROUND: Recently, urine test strip readers have become available for automated test strip analysis. We explored the possibilities of the Sysmex UC-3500 automated urine chemistry analyzer based on complementary metal oxide semiconductor (CMOS) sensor technology with regard to accuracy of leukocyte esterase and hemoglobin peroxidase results. We studied the influence of possible confounders on these measurements. METHODS: Reflectance data of leukocyte esterase and hemoglobin peroxidase were measured using CMOS technology on the Sysmex UC-3500 automated urine chemistry analyzer...
February 10, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29417012/the-reliability-and-validity-of-using-the-urine-dipstick-test-by-patient-self-assessment-for-urinary-tract-infection-screening-in-spinal-cord-injury-patients
#9
Krit Duanngai, Patpiya Sirasaporn, Siriwan Surapaitoon Ngaosinchai
Aims: The aim of this is to evaluate the reliability of the urine dipstick test by patients' self-assessment for urinary tract infection (UTI) screening and to determine the validity of urine dipstick test. Settings: Rehabilitation Department, Srinagarind Hospital, Thailand. Study Design: A diagnostic study. Subjects and Methods: This study compared the urine dipstick test (index test) with the National Institute on Disability and Rehabilitation Research (NIDRR) criteria (gold standard test) in spinal cord injury (SCI) patients...
July 2017: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/29339564/accuracy-of-the-urinalysis-for-urinary-tract-infections-in-febrile-infants-60-days-and-younger
#10
Leah Tzimenatos, Prashant Mahajan, Peter S Dayan, Melissa Vitale, James G Linakis, Stephen Blumberg, Dominic Borgialli, Richard M Ruddy, John Van Buren, Octavio Ramilo, Nathan Kuppermann
OBJECTIVES: Reports of the test accuracy of the urinalysis for diagnosing urinary tract infections (UTIs) in young febrile infants have been variable. We evaluated the test characteristics of the urinalysis for diagnosing UTIs, with and without associated bacteremia, in young febrile infants. METHODS: We performed a planned secondary analysis of data from a prospective study of febrile infants ≤60 days old at 26 emergency departments in the Pediatric Emergency Care Applied Research Network...
February 2018: Pediatrics
https://www.readbyqxmd.com/read/29332864/associations-of-periparturient-plasma-biochemical-parameters-endometrial-leukocyte-esterase-and-myeloperoxidase-and-bacterial-detection-with-clinical-and-subclinical-endometritis-in-postpartum-dairy-cows
#11
Sayed Ahmad Nazhat, Go Kitahara, Nobutoshi Kozuka, Shogo Mido, Mohammed Sadawy, Hossam El-Sheikh Ali, Takeshi Osawa
This study was aimed at demonstrating associations between peripheral biochemical parameters, endometrial leukocyte esterase (LE) and myeloperoxidase (MPO), and bacterial detection with the degree of endometrial inflammation, and determining the best time postpartum for diagnosing endometritis to predict subsequent fertility in dairy cows. Plasma albumin, blood urea nitrogen (BUN), total cholesterol (T-cho), NEFA, and BHBA concentrations were analyzed in 43 Holstein cows at 3, 5 and 7 weeks postpartum (W3, W5 and W7)...
February 20, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29298259/the-accuracy-of-the-alpha-defensin-lateral-flow-device-for-diagnosis-of-periprosthetic-joint-infection-comparison-with-a-gold-standard
#12
Thorsten Gehrke, Christian Lausmann, Mustafa Citak, Tommaso Bonanzinga, Lars Frommelt, Akos Zahar
BACKGROUND: Alpha defensin is a new biomarker that has been shown to have a very high accuracy to rule out periprosthetic joint infection. Recently, a new rapid lateral flow version of the alpha defensin test was developed and introduced to detect high levels of alpha defensin in synovial fluid quickly and with ease. We conducted a single-center prospective clinical study to compare the results of the Synovasure Alpha Defensin Test with those of the Musculoskeletal Infection Society (MSIS) criteria, which are considered to be the gold standard for diagnosing periprosthetic joint infection...
January 3, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29279452/prevalence-and-genotype-distribution-of-chlamydia-trachomatis-in-urine-among-men-attending-sexually-transmitted-disease-clinics-in-guangdong-province-china-in-2016
#13
Yaohua Xue, Heping Zheng, Weiming Tang, Zhida Mai, Jinmei Huang, Shujie Huang, Xiaolin Qin, Lei Chen, Lei Zheng
Studies have rarely assessed the genotype distribution of Chlamydia trachomatis (CT) in urine among men attending sexually transmitted disease clinics (MSCs) in China. This study was aimed at investigating the prevalence and molecular epidemiology of CT infection by examining urine samples among MSCs from different geographic areas of Guangdong Province, China. A cross-sectional study was conducted among MSCs from 10 human immunodeficiency virus sentinel surveillance sites in Guangdong Province. CT DNA was extracted from male urine samples and analyzed using a Roche cobas 4800 CT/NG...
March 22, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29214126/pharmacologic-complement-inhibition-in-clinical-transplantation
#14
REVIEW
Vasishta S Tatapudi, Robert A Montgomery
Purpose of Review: Over the past two decades, significant strides made in our understanding of the etiology of antibody-mediated rejection (AMR) in transplantation have put the complement system in the spotlight. Here, we review recent progress made in the field of pharmacologic complement inhibition in clinical transplantation and aim to understand the impact of this therapeutic approach on outcomes in transplant recipients. Recent Findings: Encouraged by the success of agents targeting the complement cascade in disorders of unrestrained complement activation like paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS), investigators are testing the safety and efficacy of pharmacologic complement blockade in mitigating allograft injury in conditions ranging from AMR to recurrent post-transplant aHUS, C3 glomerulopathies and antiphospholipid anti-body syndrome (APS)...
2017: Current Transplantation Reports
https://www.readbyqxmd.com/read/29189595/fast-and-sensitive-automated-point-of-care-urine-dips
#15
Laurie Malia, Kaitlin Strumph, Sharon Smith, John Brancato, Stephanie T Johnson, Henry Chicaiza
OBJECTIVE: Point-of-care (POC) urine dipstick is a highly used test in the pediatric emergency department (PED) owing to its fast turn-around time and inexpensive cost. Past studies have shown hand-held urine dipsticks and automated urinalysis in children younger than 48 months to be sensitive predictors for urinary tract infection (UTI). It is hypothesized that POC dip testing is as accurate as laboratory urinalysis in the diagnosis of UTI. METHODS: A retrospective chart review was conducted on patients (aged birth through 18 years) presenting to a PED between January 2015 and December 2015...
November 20, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29146619/urinary-neutrophil-gelatinase-associated-lipocalin-for-the-diagnosis-of-urinary-tract-infections
#16
Tamar R Lubell, Jonathan M Barasch, Katherine Xu, Maria Ieni, Keven I Cabrera, Peter S Dayan
OBJECTIVES: To determine the accuracy of the novel biomarker urinary neutrophil gelatinase-associated lipocalin (uNGAL) to diagnose urinary tract infections (UTIs) in febrile infants and young children. METHODS: Prospective cross-sectional study of febrile infants <3 months ( ≥ 38.0°C) and children 3 to 24 months (≥ 39.0°C) evaluated for UTIs. uNGAL levels, urinalysis, Gram-stain and culture were obtained. UTI was defined by colony counts. RESULTS: Of 260 patients, 35 (13...
December 2017: Pediatrics
https://www.readbyqxmd.com/read/29135902/should-the-absence-of-urinary-nitrite-influence-empiric-antibiotics-for-urinary-tract-infection-in-young-children
#17
Pradip P Chaudhari, Michael C Monuteaux, Richard G Bachur
OBJECTIVES: Screening for urinary tract infection (UTI) includes urinary nitrite testing by dipstick urinalysis. Gram-negative enteric organisms produce urinary nitrite and represent the most common uropathogens. Enterococcus, a less common uropathogen, does not produce nitrite and has a unique antibiotic resistance pattern. Whether to adjust empiric antibiotics in the absence of urinary nitrite has not been established. Our primary objective was to determine prevalence of enterococcal UTI among young children with a nitrite negative urinalysis...
November 14, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29099342/synovial-fluid-leukocyte-esterase-in-the-diagnosis-of-peri-prosthetic-joint-infection-a-systematic-review-and-meta-analysis
#18
Chi Wang, Rui Li, Qi Wang, Chengbin Wang
BACKGROUND: Peri-prosthetic joint infection (PJI) is a serious and frequent complication of total joint arthroplasty (TJA). Recently, synovial fluid leukocyte esterase (LE), measurement of which is convenient and fast, has been examined as a marker of PJI. We summarized the articles describing synovial fluid LE as a biomarker for the diagnosis of PJI and assessed its diagnostic value in patients suspected of having PJI. METHODS: We searched with appropriate key words in PubMed, Embase, Web of Science, the Cochrane database, and Science Direct...
November 3, 2017: Surgical Infections
https://www.readbyqxmd.com/read/29059113/the-diagnostic-utility-of-synovial-fluid-markers-in-periprosthetic-joint-infection-a-systematic-review-and-meta-analysis
#19
REVIEW
Anas Saleh, Deepak Ramanathan, Marcelo Bogliolo P Siqueira, Alison K Klika, Wael K Barsoum, Carlos A Higuera Rueda
INTRODUCTION: This study is a systematic review of all reported synovial fluid markers for the diagnosis of periprosthetic joint infection and a meta-analysis of the most frequently reported markers to identify those of greatest diagnostic utility. METHODS: A search of six databases was conducted to identify all studies evaluating the utility of synovial fluid markers in the diagnosis of periprosthetic joint infection. Two observers assessed methodologic quality and extracted data independently...
November 2017: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29020720/pyelonephritis-can-lead-to-life-threatening-complications
#20
Declan B Keenan, Declan M O'Rourke, Aisling E Courtney
Acute pyelonephritis is suggested by the constellation of fever (temperature ≥ 38.5° C), flank pain (typically unilateral), nausea and vomiting, and costovertebral angle tenderness. Complaints typical of lower UTI are variably present. The severity of symptoms ranges from a mild pyrexial illness to life-threatening sepsis. The diagnosis of acute pyelonephritis should be suspected on the basis of the history and clinical examination. If the urine dipstick is negative for nitrites and leukocyte esterase this does not exclude the diagnosis, but it should prompt a re-evaluation of the clinical features and consideration of other potential diagnoses...
February 2017: Practitioner
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