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Satish Rao

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https://www.readbyqxmd.com/read/27890954/prevalence-of-vitamin-b12-and-folic-acid-deficiency-in-hiv-positive-patients-and-its-association-with-neuropsychiatric-symptoms-and-immunological-response
#1
Prabha M R Adhikari, Mukta N Chowta, John T Ramapuram, Satish Rao, Karthik Udupa, Sahana Devdas Acharya
BACKGROUND: Deficiency of micronutrients is prevalent even before the development of symptoms of HIV disease and is associated with accelerated HIV disease progression. AIMS: This study evaluates the prevalence of folate and Vitamin B12 deficiency in HIV-positive patients with or without tuberculosis (TB) and its association with neuropsychiatric symptoms and immunological response. SETTINGS AND DESIGN: Cross-sectional, observational study in an outpatient setting...
July 2016: Indian Journal of Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27875744/identification-of-protein-secondary-structures-by-laser-induced-autofluorescence-a-study-of-urea-and-gnhcl-induced-protein-denaturation
#2
Manjunath Siddaramaiah, Kapaettu Satyamoorthy, Bola Sadashiva Satish Rao, Suparna Roy, Subhash Chandra, Krishna Kishore Mahato
In the present study an attempt has been made to interrogate the bulk secondary structures of some selected proteins (BSA, HSA, lysozyme, trypsin and ribonuclease A) under urea and GnHCl denaturation using laser induced autofluorescence. The proteins were treated with different concentrations of urea (3M, 6M, 9M) and GnHCl (2M, 4M, 6M) and the corresponding steady state autofluorescence spectra were recorded at 281nm pulsed laser excitations. The recorded fluorescence spectra of proteins were then interpreted based on the existing PDB structures of the proteins and the Trp solvent accessibility (calculated using "Scratch protein predictor" at 30% threshold)...
November 14, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27816361/genetic-variants-in-cd44-and-mat1a-confer-susceptibility-to-acute-skin-reaction-in-breast-cancer-patients-undergoing-radiation-therapy
#3
Kamalesh Dattaram Mumbrekar, Satish Rao Bola Sadashiva, Shama Prasada Kabekkodu, Donald Jerard Fernandes, Bejadi Manjunath Vadhiraja, Tomo Suga, Yoshimi Shoji, Fumiaki Nakayama, Takashi Imai, Kapaettu Satyamoorthy
PURPOSE: Heterogeneity in radiation therapy (RT)-induced normal tissue toxicity is observed in 10% of cancer patients, limiting the therapeutic outcomes. In addition to treatment-related factors, normal tissue adverse reactions also manifest from genetic alterations in distinct pathways majorly involving DNA damage-repair genes, inflammatory cytokine genes, cell cycle regulation, and antioxidant response. Therefore, the common sequence variants in these radioresponsive genes might modify the severity of normal tissue toxicity, and the identification of the same could have clinical relevance as a predictive biomarker...
September 20, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27793084/bigmac-breaking-inaccurate-genomes-and-merging-assembled-contigs-for-long-read-metagenomic-assembly
#4
Ka-Kit Lam, Richard Hall, Alicia Clum, Satish Rao
BACKGROUND: The problem of de-novo assembly for metagenomes using only long reads is gaining attention. We study whether post-processing metagenomic assemblies with the original input long reads can result in quality improvement. Previous approaches have focused on pre-processing reads and optimizing assemblers. BIGMAC takes an alternative perspective to focus on the post-processing step. RESULTS: Using both the assembled contigs and original long reads as input, BIGMAC first breaks the contigs at potentially mis-assembled locations and subsequently scaffolds contigs...
October 28, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/27579587/ethambutol-induces-testicular-damage-and-decreases-the-sperm-functional-competence-in-swiss-albino-mice
#5
Arpitha Rao, Guruprasad Nayak, Sandhya Kumari, Amratha D Prabhu, Nalini Khandige, Sneha Guruprasad Kalthur, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga
The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P<0.001) and DNA damage increased (P<0...
August 12, 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27528177/repeat-treatment-with-rifaximin-is-safe-and-effective-in-patients-with-diarrhea-predominant-irritable-bowel-syndrome
#6
Anthony Lembo, Mark Pimentel, Satish S Rao, Philip Schoenfeld, Brooks Cash, Leonard B Weinstock, Craig Paterson, Enoch Bortey, William P Forbes
BACKGROUND & AIMS: Few treatments have demonstrated efficacy and safety for diarrhea-predominant irritable bowel syndrome (IBS-D). A phase 3, randomized, double-blind, placebo-controlled trial was performed to evaluate the safety and efficacy of repeat treatment with the nonsystemic antibiotic rifaximin. METHODS: The trial included adults with IBS-D, mean abdominal pain and bloating scores of 3 or more, and loose stool, located at 270 centers in the United States and Europe from February 2012 through June 2014...
December 2016: Gastroenterology
https://www.readbyqxmd.com/read/27495130/photo-biomodulatory-response-of-low-power-laser-irradiation-on-burn-tissue-repair-in-mice
#7
Bharath Rathnakar, Bola Sadashiva Satish Rao, Vijendra Prabhu, Subhash Chandra, Sharada Rai, Anuradha Calicut Kini Rao, Mrinalini Sharma, Pradeep Kumar Gupta, Krishna Kishore Mahato
The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm(2)) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm(2)). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm(2) fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study...
December 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27487312/safety-of-completion-thyroidectomy-for-initially-misdiagnosed-thyroid-carcinoma
#8
Gangiti Kranthikumar, Nusrath Syed, Hemantkumar Nemade, Satish Pawar, L M Chandra Sekhara Rao, T Subramanyeshwar Rao
INTRODUCTION: Completion thyroidectomy is defined as the surgical removal of the remnant thyroid tissue following procedures of less than total or near-total thyroidectomy. Whether thyroid reoperations are associated with an increased complication risk is controversial. OBJECTIVE: A retrospective analysis was done of patients undergoing completion thyroidectomy for cancer of the thyroid who had undergone surgery elsewhere for solitary thyroid nodule. The incidence of surgical complications in these patients after reoperation was investigated in this study...
2016: Rambam Maimonides Medical Journal
https://www.readbyqxmd.com/read/27467820/update-interim-guidance-for-health-care-providers-caring-for-pregnant-women-with-possible-zika-virus-exposure-united-states-july-2016
#9
Titilope Oduyebo, Irogue Igbinosa, Emily E Petersen, Kara N D Polen, Satish K Pillai, Elizabeth C Ailes, Julie M Villanueva, Kim Newsome, Marc Fischer, Priya M Gupta, Ann M Powers, Margaret Lampe, Susan Hills, Kathryn E Arnold, Laura E Rose, Carrie K Shapiro-Mendoza, Charles B Beard, Jorge L Muñoz, Carol Y Rao, Dana Meaney-Delman, Denise J Jamieson, Margaret A Honein
CDC has updated its interim guidance for U.S. health care providers caring for pregnant women with possible Zika virus exposure, to include the emerging data indicating that Zika virus RNA can be detected for prolonged periods in some pregnant women. To increase the proportion of pregnant women with Zika virus infection who receive a definitive diagnosis, CDC recommends expanding real-time reverse transcription-polymerase chain reaction (rRT-PCR) testing. Possible exposures to Zika virus include travel to or residence in an area with active Zika virus transmission, or sex* with a partner who has traveled to or resides in an area with active Zika virus transmission without using condoms or other barrier methods to prevent infection...
2016: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/27437708/intra-specific-differentiation-of-fungal-endosymbiont-alternaria-longissima-clb44-using-rna-secondary-structure-analysis-and-their-anti-infective-potential
#10
H C Yashavantha Rao, Sreedharamurthy Satish
New antimicrobial agents derived from endosymbio-tic fungi with unique and targeted mode of action are crucially rudimentary to combat multidrug-resistant infections. Most of the fungi isolated as endosymbionts show close morphological feature resemblance to plant pathogenic or free-living forms, and it is difficult to differentiate these different lifestyles. A fungal endosymbiont strain CLB44 was isolated from Combretum latifolium Blume (Combretaceae). CLB44 was then identified as Alternaria longissima based on morphological and internal transcribed spacer (ITS) intervening 5...
August 2016: Die Naturwissenschaften
https://www.readbyqxmd.com/read/27403378/biomimetic-synthesis-of-silver-nanoparticles-using-endosymbiotic-bacterium-inhabiting-euphorbia-hirta-l-and-their-bactericidal-potential
#11
Baker Syed, Hoovinakola Chinnappa Yashavantha Rao, Mysore Nagalingaswamy Nagendra-Prasad, Ashwini Prasad, Ballagere Puttaraju Harini, Pasha Azmath, Devaraju Rakshith, Sreedharamurthy Satish
The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques...
2016: Scientifica
https://www.readbyqxmd.com/read/27365952/effect-of-vitamin-b12-and-folic-acid-supplementation-on-neuropsychiatric-symptoms-and-immune-response-in-hiv-positive-patients
#12
Prabha M Adhikari, Mukta N Chowta, John T Ramapuram, Satish B Rao, Karthik Udupa, Sahana D Acharya
BACKGROUND: Micronutrients such as B12 and folic acid deficiencies are found in higher number in HIV-infected patients. OBJECTIVE: We conducted a study to examine the effect of Vitamin B12 and folic acid supplementation on neuropsychiatric manifestations, CD4 count, and anthropometric measurements in HIV-positive patients. MATERIALS AND METHODS: Three different groups of HIV patients, namely, HIV patients with tuberculosis, HIV patients with neuropsychiatric manifestations, and asymptomatic HIV patients with 50 patients in each group were included in the study...
July 2016: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/27270989/diagnosis-and-treatment-of-dyssynergic-defecation
#13
REVIEW
Satish S C Rao, Tanisa Patcharatrakul
Dyssynergic defecation is common and affects up to one half of patients with chronic constipation. This acquired behavioral problem is due to the inability to coordinate the abdominal and pelvic floor muscles to evacuate stools. A detailed history, prospective stool diaries, and a careful digital rectal examination will not only identify the nature of bowel dysfunction, but also raise the index of suspicion for this evacuation disorder. Anorectal physiology tests and balloon expulsion test are essential for a diagnosis...
July 30, 2016: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/27236214/-polymorphisms-in-double-strand-break-repair-related-genes-influence-radiosensitivity-phenotype-in-lymphocytes-from-healthy-individuals-dna-repair-40-2016-27-34
#14
Kamalesh Dattaram Mumbrekar, Hassan Venkatesh Goutham, Bejadi Manjunath Vadhiraja, Satish Rao Bola Sadashiva
No abstract text is available yet for this article.
May 25, 2016: DNA Repair
https://www.readbyqxmd.com/read/27162571/transorbital-orbitocranial-penetrating-injury-with-an-iron-rod
#15
Amit Agrawal, V Umamaheswara Reddy, S Satish Kumar, Kishor V Hegde, G Malleswara Rao
Transorbital orbitocranial penetrating injuries (TOPIs) are relatively rare, can be caused by high-speed projectile foreign bodies to low-energy trauma (which is rarer), and account for 24% of penetrating head injuries in adults and approximately 45% in children. We report an uncommon nonfatal case of TOPI where a 16-year-old male child sustained injury due to accidental penetration of metal bar into the forehead. A bicoronal flap was raised to remove the metal bar. The patient recovered well, had normal vision, and doing well at follow-up...
June 2016: Craniomaxillofacial Trauma & Reconstruction
https://www.readbyqxmd.com/read/27154505/implication-of-pks-type-i-gene-and-chromatographic-strategy-for-the-biodiscovery-of-antimicrobial-polyketide-metabolites-from-endosymbiotic-nocardiopsis-prasina-cla68
#16
H C Yashavantha Rao, Devaraju Rakshith, D M Gurudatt, Sreedharamurthy Satish
Advanced approach in probing for polyketide antimicrobials requires novel genomics and chromatographic strategies. An endophytic strain CLA68 was isolated from the root of Combretum latifolium Blume (Combretaceae) collected from the Western Ghats of Southern India. Strain CLA68 was then identified as Nocardiopsis prasina by its characteristic culture morphology and analysis of 16S rRNA gene sequence. Biosynthetic polyketide synthase genes were investigated using two pairs of degenerate primers. Ethyl acetate extract of CLA68 exhibited broad spectrum activity against a panel of test human pathogens...
June 2016: Die Naturwissenschaften
https://www.readbyqxmd.com/read/27144634/rome-iv-diagnostic-questionnaires-and-tables-for-investigators-and-clinicians
#17
Olafur S Palsson, William E Whitehead, Miranda A L van Tilburg, Lin Chang, William Chey, Michael D Crowell, Laurie Keefer, Anthony J Lembo, Henry P Parkman, Satish Sc Rao, Ami Sperber, Brennan Spiegel, Jan Tack, Stephen Vanner, Lynn S Walker, Peter Whorwell, Yunsheng Yang
The Rome IV Diagnostic Questionnaires were developed to screen for functional gastrointestinal disorders (FGIDs), serve as inclusion criteria in clinical trials, and support epidemiological surveys. Separate questionnaires were developed for adults, children/adolescents, and infants/toddlers. For the adult questionnaire, we first surveyed 1,162 adults without gastrointestinal disorders, and recommended the 90(th) percentile symptom frequency as the threshold for defining what is abnormal. Diagnostic questions were formulated and verified with clinical experts using a recursive process...
February 13, 2016: Gastroenterology
https://www.readbyqxmd.com/read/27144630/functional-anorectal-disorders
#18
Satish Sc Rao, Adil E Bharucha, Giuseppe Chiarioni, Richelle Felt-Bersma, Charles Knowles, Allison Malcolm, Arnold Wald
This report defines criteria and reviews the epidemiology, pathophysiology, and management of common anorectal disorders: fecal incontinence (FI), functional anorectal pain and functional defecation disorders. FI is defined as the recurrent uncontrolled passage of fecal material for at least 3 months. The clinical features of FI are useful for guiding diagnostic testing and therapy. Anorectal manometry and imaging are useful for evaluating anal and pelvic floor structure and function. Education, antidiarrheals and biofeedback therapy are the mainstay of management; surgery may be useful in refractory cases...
March 25, 2016: Gastroenterology
https://www.readbyqxmd.com/read/27134590/a-review-of-esophageal-chest-pain
#19
Enrique Coss-Adame, Satish S C Rao
Noncardiac chest pain is a term that encompasses all causes of chest pain after a cardiac source has been excluded. This article focuses on esophageal sources for chest pain. Esophageal chest pain (ECP) is common, affects quality of life, and carries a substantial health care burden. The lack of a systematic approach toward the diagnosis and treatment of ECP has led to significant disability and increased health care costs for this condition. Identifying the underlying cause(s) or mechanism(s) for chest pain is key for its successful management...
November 2015: Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27090893/losartan-attenuates-degradation-of-aorta-and-lung-tissue-micromechanics-in-a-mouse-model-of-severe-marfan-syndrome
#20
Jia-Jye Lee, Josephine Galatioto, Satish Rao, Francesco Ramirez, Kevin D Costa
Marfan syndrome (MFS) is an autosomal dominant disease of the connective tissue due to mutations in the fibrillin-1 gene (FBN1). This study aimed at characterizing microelastic properties of the ascending aortic wall and lung parenchyma tissues from wild type (WT) and age-matched Fbn1 hypomorphic mice (Fbn1(mgR/mgR) mice) to identify tissue-specific biomechanical effects of aging and disease in MFS. Atomic force microscopy was used to indent lung parenchyma and aortic wall tissues, using Hybrid Eshelby Decomposition analysis to extract layer-specific properties of the intima and media...
October 2016: Annals of Biomedical Engineering
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