keyword
MENU ▼
Read by QxMD icon Read
search

cell and gene therapy

keyword
https://www.readbyqxmd.com/read/29656061/hypoxia-inducible-bidirectional-shrna-expression-vector-delivery-using-pei-chitosan-tba-copolymers-for-colorectal-cancer-gene-therapy
#1
Bita Javan, Fatemeh Atyabi, Majid Shahbazi
AIMS: This investigation was conducted to construct a hypoxia/colorectal dual-specific bidirectional short hairpin RNA (shRNA) expression vector and to transfect it into the colon cancer cell line HT-29 with PEI/chitosan-TBA nanoparticles for the simultaneous knock down of β-catenin and Bcl-2 under hypoxia. MAIN METHODS: To construct a pRNA-bipHRE-CEA vector, the carcinoma embryonic antigen (CEA) promoter designed in two directions and the vascular endothelial growth factor (VEGF) enhancer were inserted between two promoters for hypoxic cancer specific gene expression...
April 12, 2018: Life Sciences
https://www.readbyqxmd.com/read/29655990/inorganic-kernel-supported-asymmetric-hybrid-vesicles-for-targeting-delivery-of-stat3-decoy-oligonucleotides-to-overcome-anti-her2-therapeutic-resistance-of-bt474r
#2
Kai Shi, Yan Fang, Shan Gao, Dongjuan Yang, Hongshu Bi, Jianxiu Xue, Anqi Lu, Yuai Li, Liyuan Ke, Xiaojie Lin, Xuechao Jin, Min Li
As a recombinant humanized monoclonal antibody that targets the extracellular region of HER2 tyrosine kinase receptor, trastuzumab (TRAZ) has demonstrated comparable clinical efficacy and improved survival in patients with HER2-positive breast cancer. Nevertheless, the therapeutic potential of TRAZ is often limited due to its frequent resistance to anti-HER2 therapy. Therefore, we investigate the reversal effect of STAT3-specific decoy oligonucleotides (STAT3-decoy ODNs) on TRAZ resistance, which contain the consensus sequence within the targeted gene promoter of STAT3...
April 12, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29655989/in-vivo-migration-of-mesenchymal-stem-cells-to-burn-injury-sites-and-their-therapeutic-effects-in-a-living-mouse-model
#3
Eun Jung Oh, Ho Won Lee, Senthilkumar Kalimuthu, Tae Jung Kim, Hyun Mi Kim, Se Hwan Baek, Liya Zhu, Ji Min Oh, Seung Hyun Son, Ho Yun Chung, Byeong-Cheol Ahn
Mesenchymal stem cell (MSC)-based therapy has emerged as a promising therapeutic strategy for tissue regeneration and repair. In this study, we non-invasively monitored the tracking of MSCs toward burn injury sites using MSCs expressing firefly luciferase (Fluc) gene in living mice, and evaluated the effects of the MSCs at the injury site. Murine MSCs co-expressing Fluc and green fluorescent protein (GFP) were established using a retroviral system (referred to as MSC/Fluc). To evaluate the ability of MSC migration toward burn injury sites, cutaneous burn injury was induced in the dorsal skin of mice...
April 12, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29655638/attenuation-of-the-niemann-pick-type-c2-disease-phenotype-by-intracisternal-administration-of-an-aavrh-10-vector-expressing-npc2
#4
Sandra Markmann, Jasmine Reid, Jonathan B Rosenberg, Bishnu P De, Stephen M Kaminsky, Ronald G Crystal, Dolan Sondhi
Niemann-Pick type C2 (NPC2) disease is a rare, neurodegenerative disorder caused by mutations in the NPC2 gene, leading to lysosomal accumulation of unesterified cholesterol and other lipids. It is characterized by hepatosplenomegaly, liver dysfunction and severe neurological manifestations, resulting in early death. There is no effective therapy for NPC2 disease. Here, we evaluated the effectiveness of an adeno-associated virus (AAV), serotype rh.10 gene transfer vector expressing the mouse Npc2 gene (AAVrh...
April 12, 2018: Experimental Neurology
https://www.readbyqxmd.com/read/29653206/late-effects-screening-guidelines-after-hematopoietic-cell-transplantation-hct-for-hemoglobinopathy-consensus-statement-from-the-second-pediatric-blood-and-marrow-transplant-consortium-international-conference-on-late-effects-after-pediatric-hct
#5
Shalini Shenoy, Javid Gaziev, Emanuele Angelucci, Allison King, Monica Bhatia, Angela Smith, Dorine Bresters, Anne E Haight, Christine N Duncan, Josu de la Fuente, Andrew C Dietz, K Scott Baker, Michael A Pulsipher, Mark C Walters
Allogeneic hematopoietic cell transplantation (HCT) can halt organ damage and eliminate symptoms in hemoglobin disorders, including sickle cell disease (SCD) and thalassemia major (TM). Managing the residual manifestations of pre-HCT disease complications and the long-term effects of HCT requires systematic monitoring, follow-up, and intervention when indicated. Late complications vary with age and disease status at HCT, and with transplant variables such as preparative regimen, donor source and compatibility, and immune reconstitution...
April 10, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29653152/low-dose-norfloxacin-treated-leptospires-induce-less-il-1%C3%AE-release-in-j774a-1-cells-following-discrepant-leptospiral-gene-expression
#6
Yongguo Cao, Xufeng Xie, Wenlong Zhang, Dianjun Wu, Changchun Tu
Currently, accumulating evidence is challenging subtherapeutic therapy. Low-dose Norfloxacin (Nor) has been reported to suppress the immune response and worsen leptospirosis. In this study, we investigated the influence of low-dose Nor (0.03 μg/ml, 0.06 μg/ml, 0.125 μg/ml) on leptospiral gene expression and analyzed the immunomodulatory effects of low-dose Nor-treated leptospires in J774A.1 cells. To study the expression profiles of low-dose Nor-treated leptospires, we chose LipL71/LipL21 as reference genes determined by the geNorm applet in this experiment...
April 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29653048/different-length-hydrazone-activated-polymers-for-plasmid-dna-condensation-and-cellular-transfection
#7
Juan M Priegue, Irene Lostalé-Seijo, Daniel Crisan, Juan R Granja, Francisco Fernández-Trillo, Javier Montenegro
The recent advances in genetic engineering demand the development of conceptually new methods to prepare and identify efficient vectors for the intracellular delivery of different nucleotide payloads ranging from short single-stranded oligonucleotides to larger plasmid double-stranded circular DNAs. Although many challenges still have to be overcome, polymers hold great potential for intracellular nucleotide delivery and gene therapy. We here develop and apply the post-polymerization modification of polyhydrazide scaffolds, with different degree of polymerization, for the in situ preparation of amphiphilic polymeric vehicles for the intracellular delivery of a circular plasmid DNA...
April 13, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29652798/the-double-face-of-exosome-carried-micrornas-in-cancer-immunomodulation
#8
REVIEW
Romina Alfonsi, Ludovica Grassi, Michele Signore, Désirée Bonci
In recent years many articles have underlined the key role of nanovesicles, i.e., exosomes, as information carriers among biological systems including cancer. Tumor-derived exosomes (TEXs) are key players in the dynamic crosstalk between cancer cells and the microenvironment while promote immune system control evasion. In fact, tumors are undoubtedly capable of silencing the immune response through multiple mechanisms, including the release of exosomes. TEXs have been shown to boost tumor growth and promote progression and metastatic spreading via suppression or stimulation of the immune response towards cancer cells...
April 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29652478/isothermal-self-assembly-of-spermidine-dna-nanostructure-complex-as-a-functional-platform-for-cancer-therapy
#9
Dong Wang, Qian Liu, Di Wu, Binfeng He, Jin Li, Chengde Mao, Guansong Wang, Hang Qian
Programmable DNA nanostructure self-assembly offer great potentials in nanomedicine, drug delivery, biosensing and bioimaging. However, the intrinsically negative-charged DNA backbones, the instability of DNA nanostructures in physiological settings pose serious challenges to their practical applications. To overcome this challenge, a strategy that combines the magnesium-free DNA self-assembly and functionalization is proposed in this study. We hypothesize that naturally abundant spermidine may not only mediate the self-assembly of DNA nanostructures, but also shield them from harsh physiological environments...
April 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29651947/transplantation-of-bdnf-gene-recombinant-mesenchymal-stem-cells-and-adhesive-peptide-modified-hydrogel-scaffold-for-spinal-cord-repair
#10
Li-Ming Li, Ling-Ling Huang, Xin-Chi Jiang, Jia-Chen Chen, Hong-Wei OuYang, Jian-Qing Gao
Mesenchymal stem cells (MSCs) are promising candidates for nerve tissue engineering. Brain derived neurotrophic factor (BDNF) secreted by MSCs can function to increase neural differentiation and relieve inflammation response. Gene transfection technology is an efficient strategy to increase the secretion levels of cytokines and enhance cellular functions. However, transfection and in vivo gene expression of environmentally sensitive stem cells have been one of the most challenging subjects due to the requirement in both safety and transfection efficiency...
April 13, 2018: Current Gene Therapy
https://www.readbyqxmd.com/read/29651840/glucosamine-based-supramolecular-nanotubes-for-human-mesenchymal-cell-therapy
#11
Satish Talloj, Bill Cheng, Jen-Po Weng, Hsin-Chieh Lin
Herein, we demonstrate an example of glucosamine-based supramolecular hydrogels that can be used for human mesenchymal cell therapy. We designed and synthesized a series of amino acid derivatives based on a strategy of capping D-glucosamine moiety at C-terminus and fluorinated benzyl group at N-terminus. From a systematic study on chemical structures, we discovered that the glucosamine-based supramolecular hydrogel (PFB-F-Glu) self-assembled with one dimensional nanotubular structures at physiological pH. The self-assembly of newly discovered PFB-F-Glu motif is attributed to synergistic effect of π-π stacking and extensive intermolecular hydrogen bonding network in aqueous medium...
April 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29651410/analysis-of-micrornas-expression-profiles-in-madin-darby-bovine-kidney-cells-infected-with-caprine-parainfluenza-virus-type-3
#12
Jizong Li, Li Mao, Wenliang Li, Fei Hao, Chunyan Zhong, Xing Zhu, Xinqin Ji, Leilei Yang, Wenwen Zhang, Maojun Liu, Jieyuan Jiang
Caprine parainfluenza virus type 3 (CPIV3) is a newly emerging pathogenic respiratory agent infecting both young and adult goats, and it was identified in eastern China in 2013. Cellular microRNAs (miRNAs) have been reported to be important modulators of the intricate virus-host interactions. In order to elucidate the role of miRNAs in madin-darby bovine kidney (MDBK) cells during CPIV3 infection. In this study, we performed high-throughput sequencing technology to analyze small RNA libraries in CPIV3-infected and mock-infected MDBK cells...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29651367/-lazarus-response-to-olaparib-in-a-virtually-chemonaive-breast-cancer-patient-carrying-gross-brca2-gene-deletion
#13
Vladimir M Moiseyenko, Vyacheslav A Chubenko, Fedor V Moiseyenko, Lyudmila A Zagorskaya, Yuliya A Zaytseva, Nataliya E Gesha, Evgeny N Zykov, Valeriya I Ni, Elena V Preobrazhenskaya, Anna P Sokolenko, Evgeny N Imyanitov
This report describes an estrogen receptor-positive breast cancer patient, who relapsed at two and a half years after the completion of adjuvant chemotherapy while being on the aromatase inhibition. Based on the clinical evidence for potential sensitivity of the tumor to hormone ablation, everolimus was added to continuing exemestane treatment. Oral chemotherapy was administered at further disease progression, however, it lasted only for 10 days due to rapidly deteriorating condition of the patient. BRCA test was performed just before the failure of endocrine therapy and revealed a gross deletion within BRCA2 gene...
February 4, 2018: Curēus
https://www.readbyqxmd.com/read/29651283/mirnas-in-tuberculosis-new-avenues-for-diagnosis-and-host-directed-therapy
#14
REVIEW
Naveed Sabir, Tariq Hussain, Syed Zahid Ali Shah, Antonio Peramo, Deming Zhao, Xiangmei Zhou
Tuberculosis (TB) is one of the most fatal infectious diseases and a leading cause of mortality, with 95% of these deaths occurring in developing countries. The causative agent, Mycobacterium tuberculosis ( Mtb ), has a well-established ability to circumvent the host's immune system for its intracellular survival. microRNAs (miRNAs) are small, non-coding RNAs having an important function at the post-transcriptional level and are involved in shaping immunity by regulating the repertoire of genes expressed in immune cells...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29651051/collagen-iv-conveyed-signals-can-regulate-chemokine-production-and-promote-liver-metastasis
#15
George Vaniotis, Roni F Rayes, Shu Qi, Simon Milette, Ni Wang, Stephanie Perrino, France Bourdeau, Hanna Nyström, Yi He, Nathalie Lamarche-Vane, Pnina Brodt
Liver metastases remain a major cause of death from gastrointestinal tract cancers as well as from other malignancies such as breast and lung carcinomas and melanoma. Understanding the underlying biology is essential for the design of effective targeted therapies. We previously reported that collagen IV α1/α2 overexpression in non-metastatic lung carcinoma (M27colIV ) cells increased their metastatic ability, specifically to the liver and documented high collagen IV levels in surgical resections of liver metastases from diverse tumor types...
April 13, 2018: Oncogene
https://www.readbyqxmd.com/read/29650893/-towards-development-of-innovative-cancer-therapies-trans-omics-approach
#16
Reika Kawabata-Iwakawa, Masahiko Nishiyama
Comprehensive genomic and transcriptome analyses using next-generation sequencing(NGS)analysis has lead a discovery of a variety of novel driver gene mutations and new therapeutic targets for cancer patients, and has remarkably improved outcome of the patients through the development novel molecular targeting drugs. Even so, in so-called intractable or refractory cancers, those "druggable"alterations common to the diseases are rarely found due to the high diversity of the tumor. Furthermore, most of molecular target therapy is known to acquire the resistance to the drug by means of multiple factors such as up-regulation of the partially inhibited pathway, mutation of the target, activation of alternative pathways, histological translocation, and oncogene de-addiction...
March 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29650694/lymphatic-vasculature-requires-er-estrogen-receptor-%C3%AE-signaling-to-protect-from-lymphedema
#17
Florent Morfoisse, Florence Tatin, Benoit Chapût, Nicole Therville, Charlotte Vaysse, Raphael Métivier, Julie Malloizel-Delaunay, Francoise Pujol, Anne-Claire Godet, Fabienne De Toni, Frederic Boudou, Katia Grenier, David Dubuc, Eric Lacazette, Anne-Catherine Prats, Julie Guillermet-Guibert, Francoise Lenfant, Barbara Garmy-Susini
OBJECTIVE: Estrogens exert beneficial effect on the blood vascular system. However, their role on the lymphatic system has been poorly investigated. We studied the protective effect of the 17β estradiol-the most potent endogenous estrogen-in lymphedema-a lymphatic dysfunction, which results in a massive fluid and fat accumulation in the limb. APPROACH AND RESULTS: Screening of DNA motifs able to mobilize ERs (estrogen receptors) and quantitative real-time polymerase chain reaction analysis revealed that estradiol promotes transcriptional activation of lymphangiogenesis-related gene expression including VEGF (vascular endothelial growth factor)-D, VEGFR (VEGF receptor)-3, lyve-1, and HASs (hyaluronan synthases)...
April 12, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29650692/microrna-mediated-therapy-modulating-blood-brain-barrier-disruption-improves-vascular-cognitive-impairment
#18
Kensuke Toyama, Joshua M Spin, Alicia C Deng, Ting-Ting Huang, Ke Wei, Markus U Wagenhäuser, Takuya Yoshino, Huy Nguyen, Joscha Mulorz, Soumajit Kundu, Uwe Raaz, Matti Adam, Isabel N Schellinger, Ann Jagger, Philip S Tsao
OBJECTIVE: There are currently no effective treatments for the prevention of dementia associated with vascular cognitive impairment. MicroRNAs regulate gene expression at the post-transcriptional level and play key roles in vascular disorders. TNFα (tumor necrosis factor-α) regulates blood-brain barrier breakdown through modification of cerebral tight junctions. Here, we sought key TNFα-responsive microRNAs that might influence blood-brain barrier breakdown via cerebral tight junction disruption in vascular cognitive impairment...
April 12, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29650420/denosumab-treatment-of-inoperable-or-locally-advanced-giant-cell-tumor-of-bone-multicenter-analysis-outside-clinical-trial
#19
Piotr Rutkowski, Louie Gaston, Aneta Borkowska, Silvia Stacchiotti, Hans Gelderblom, Giacomo Giulio Baldi, Emanuela Palmerini, Paolo Casali, Alessandro Gronchi, Michael Parry, Domenico Andrea Campanacci, Guido Scoccianti, Michal Wagrodzki, Stefano Ferrari, Sander Dijkstra, Andrzej Pieńkowski, Robert Grimer
BACKGROUND: Giant cell tumor of bone (GCTB) is an osteolytic, locally aggressive, rarely metastazing bone tumor. This is a retrospective study evaluating a large series of GCTB patients treated with denosumab in routine practice in 6 European reference centers. METHODS: Patients with locally advanced, unresectable or metastatic GCTB, treated with denosumab outside clinical trials were eligible. Primary end-point was progression-free survival (PFS) for all patients; secondary end-points were: type of surgery, relapse rate and event-free survival for patients after preoperative denosumab + surgery...
March 31, 2018: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29650252/the-interferon-stimulated-gene-trim22-a-double-edged-sword-in-hiv-1-infection
#20
REVIEW
Elisa Vicenzi, Guido Poli
Infection of target cells by the human immunodeficiency virus type-1 (HIV-1) is hampered by constitutively expressed host cell proteins preventing or curtailing virus replication and therefore defined as "restriction factors". Among them, members of the tripartite motif (TRIM) family have emerged as important players endowed with both antiviral effects and modulatory capacity of the innate immune response. TRIM5α and TRIM19 (i.e. promyelocytic leukemia, PML) are among the best-characterized family members; however, in this review we will focus on the potential role of another family member, i...
February 10, 2018: Cytokine & Growth Factor Reviews
keyword
keyword
111041
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"