keyword
MENU ▼
Read by QxMD icon Read
search

igg3

keyword
https://www.readbyqxmd.com/read/28334051/dissecting-the-relationships-of-igg-subclasses-and-complements-in-membranous-lupus-nephritis-and-idiopathic-membranous-nephropathy
#1
Woong Na, Kijong Yi, Young Soo Song, Moon Hyang Park
Membranous lupus nephritis (MLN) and idiopathic membranous nephropathy (IMN) are kidney diseases with similar morphology, but distinct etiologies, both producing glomeruli with immune deposits. Immunoglobulins and complements, the main components of the deposits, can be detected by immunofluorescence (IF) microscopy. Previous researches characterized the immune deposits only individually, but not the interactions between them. To study these relationships we analyzed an IF profile of IgG subclasses and complements (IgG1, IgG2, IgG3, IgG4, C3, C1q, and C4) in 53 and 95 cases of biopsy-confirmed MLNs and IMNs, respectively, mainly using information theory and Bayesian networks...
2017: PloS One
https://www.readbyqxmd.com/read/28329101/naturally-acquired-antibodies-target-the-glutamate-rich-protein-on-intact-merozoites-and-predict-protection-against-febrile-malaria
#2
Ikhlaq Hussain Kana, Bright Adu, Régis Wendpayangde Tiendrebeogo, Susheel Kumar Singh, Daniel Dodoo, Michael Theisen
Background.: Plasmodium species antigens accessible at the time of merozoite release are likely targets of biologically functional antibodies. Methods.: Immunoglobulin G (IgG) antibodies against intact merozoites were quantified in the plasma of Ghanaian children from a longitudinal cohort using a novel flow cytometry-based immunofluorescence assay. Functionality of these antibodies, as well as glutamate-rich protein (GLURP)-specific affinity-purified IgG from malaria hyperimmune Liberian adults, was assessed by the opsonic phagocytosis (OP) assay...
February 21, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28325049/antibody-subclass-detection-using-graphene-nanopore
#3
Amir Barati Farimani, Mohammad Heiranian, Kyoungmin Min, Narayana R Aluru
Solid-state nanopores are promising for label free protein detection. The large thickness, ranging from several tens of nanometers to micrometers and larger, of solid-state nanopores prohibits atomic scale scanning or interrogation of proteins. Here, a single-atom thick graphene nanopore is shown to be highly capable of sensing and discriminating between different subclasses of IgG antibodies despite their minor and subtle variation in atomic structure. Extensive molecular dynamics (MD) simulations, rigorous statistical analysis with a total aggregate simulation time of 2...
March 21, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28315348/fc-gamma-receptor-3a-polymorphism-predicts-the-incidence-of-urinary-tract-infection-in-kidney-transplant-recipients
#4
Lalit Kumar Das, Kentaro Ide, Asuka Tanaka, Hiroshi Morimoto, Seiichi Shimizu, Naoki Tanimine, Yuka Tanaka, Hideki Ohdan
We investigated the impact of polymorphisms in host innate immunoregulatory genes on the development of infectious complications after kidney transplantation (KT). The single-nucleotide polymorphisms (SNPs) of C1QA [276 A/G], FCGR2A [131 H/R], and FCGR3A [158 F/V], genes encoding the Fc gamma receptor (FcγR), were analyzed in 81 KT recipients in relation to the occurrences of postoperative infectious complications within 30 days after KT. Consistent with a lower affinity of the isoform encoded by the FCGR3A [158 F] to both IgG1 and IgG3, a significantly higher incidence of urinary tract infections (UTIs) was observed in the FCGR3A [158 F/V or F/F] individuals (65...
March 14, 2017: Human Immunology
https://www.readbyqxmd.com/read/28219072/human-allergen-specific-igg-subclass-antibodies-measured-using-immunocap-technology
#5
Robert Movérare, Karin Blume, Peter Lind, René Crevel, Åsa Marknell DeWitt, Stella Cochrane
BACKGROUND: Knowledge of human IgG subclass antibody responses to various allergens has been hampered by a lack of reliable standardized assays. The aim here was to develop quantitative immunoassays for human IgG1, IgG2, and IgG3 antibodies using ImmunoCAP® technology and to evaluate their application. METHODS: Enzyme conjugates with isotype-specific monoclonal antibodies and calibrators composed of purified myeloma paraproteins were developed for each assay and used together with other standardized assay reagents for the Phadia® 100 instrument...
2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28177177/purification-and-quantification-of-heavy-chain-antibodies-from-the-milk-of-bactrian-camels
#6
Hongqiang Yao, Min Zhang, Yi Li, Jirimutu Yao, He Meng, Siriguleng Yu
Camel milk has a unique composition with naturally occurring heavy-chain antibodies (HCAbs), which exert rehabilitating potencies in infection and immunity. To characterize HCAb in camel milk, immunoglobulin G (IgG) was isolated from the milk of Camelus bactrianus by a combination of affinity chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis to purify and size-fractionate protein A and protein G, which were further identified by Western blotting, and were quantified by bicinchoninic acid (BCA) and ELISA...
February 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28159571/igg3-snitcher-of-rsv-infections-in-the-very-young
#7
Bert Schepens, Xavier Saelens
No abstract text is available yet for this article.
January 30, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28125577/identification-of-three-immunodominant-motifs-with-atypical-isotype-profile-scattered-over-the-onchocerca-volvulus-proteome
#8
Ole Lagatie, Bieke Van Dorst, Lieven J Stuyver
Understanding the immune response upon infection with the filarial nematode Onchocerca volvulus and the mechanisms that evolved in this parasite to evade immune mediated elimination is essential to expand the toolbox available for diagnostics, therapeutics and vaccines development. Using high-density peptide microarrays we scanned the proteome-wide linear epitope repertoire in Cameroonian onchocerciasis patients and healthy controls from Southern Africa which led to the identification of 249 immunodominant antigenic peptides...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28122974/monoclonal-antibodies-to-v2-v3-the-cd4-binding-site-and-gp41-hiv-1-mediate-phagocytosis-in-a-dose-dependent-manner
#9
Thomas Musich, Liuzhe Li, Lily Liu, Susan Zolla-Pazner, Marjorie Robert-Guroff, Miroslaw K Gorny
In light of weak or absent neutralizing activity mediated by anti-V2 monoclonal Abs (mAbs), we tested whether they can mediate Ab-dependent cellular phagocytosis (ADCP) which is an important element of anti-HIV-1 immunity. We tested six anti-V2 mAbs and compared them with 21 mAbs specific for V3, the CD4-binding site (CD4bs), and gp41 derived from HIV-1 chronically infected individuals and produced by hybridoma cells. ADCP activity was measured by flow cytometry using uptake by THP-1 monocytic cells of fluorescent beads coated with gp120, gp41, BG505 SOSIP...
January 25, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28121454/igg-antibody-response-elicited-by-a-fully-synthetic-two-component-carbohydrate-based-cancer-vaccine-candidate-with-%C3%AE-galactosylceramide-as-built-in-adjuvant
#10
Xu-Guang Yin, Xiang-Zhao Chen, Wen-Mei Sun, Xiao-Shan Geng, Xiao-Kang Zhang, Jian Wang, Pan-Pan Ji, Zhong-Yin Zhou, Dong Jae Baek, Guang-Fu Yang, Zheng Liu, Jun Guo
A fully synthetic self-adjuvanting cancer vaccine candidate was constructed through covalent conjugation of invariant natural killer T (iNKT) cell ligand α-galactosylceramide (αGalCer) with sialyl Tn (STn), a representative tumor-associated carbohydrate antigen (TACA). This two-component vaccine STn-αGalCer is devoid of antigenic peptide, featuring the well-defined structure with high simplicity. STn-αGalCer showed remarkable efficacy in inducing antibody class switching from IgM to STn-specific IgG. Subtypes of IgG antibody were primarily IgG1 and IgG3...
January 25, 2017: Organic Letters
https://www.readbyqxmd.com/read/28118850/humans-from-wuchereria-bancrofti-endemic-area-elicit-substantial-immune-response-to-proteins-of-the-filarial-parasite-brugia-malayi-and-its-endosymbiont-wolbachia
#11
Ruchi Jha, Mamta Gangwar, Dhanvantri Chahar, Anand Setty Balakrishnan, Mahendra Pal Singh Negi, Shailja Misra-Bhattacharya
BACKGROUND: In the past, immune responses to several Brugia malayi immunodominant antigens have been characterized in filaria-infected populations; however, little is known regarding Wolbachia proteins. We earlier cloned and characterized few B. malayi (trehalose-6-phosphate phosphatase, Bm-TPP and heavy chain myosin, BmAF-Myo) and Wolbachia (translation initiation factor-1, Wol Tl IF-1 and NAD(+)-dependent DNA ligase, wBm-LigA) proteins and investigated the immune responses, which they triggered in animal models...
January 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28115508/eye-drop-inoculation-leads-to-sublethal-leptospirosis-in-mice
#12
Joseph Pierce Sullivan, Nisha Nair, Hari-Hara Potula, Maria Gomes-Solecki
Leptospirosis is potentially a fatal zoonosis acquired by contact of skin and mucosal surfaces with soil and water contaminated with infected urine. We analyzed the outcome of infection of C3H-HeJ mice with Leptospira interrogans serovar Copenhageni, using an enzootic mode of transmission, the conjunctival route. Infection led to weight loss and L. interrogans dissemination from blood to urine. However, the sequence of these events occurred later and were not as clearly separated in time given that spirochete detection overlapped in both tissues...
January 23, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28111238/age-specific-profiles-of-antibody-responses-against-respiratory-syncytial-virus-infection
#13
Nao Jounai, Megumi Yoshioka, Miyuki Tozuka, Kazue Inoue, Tatsuya Oka, Kazuki Miyaji, Katsuyasu Ishida, Naoki Kawai, Hideyuki Ikematsu, Chiaki Kawakami, Hiroyuki Shimizu, Masaaki Mori, Ken J Ishii, Fumihiko Takeshita
Respiratory syncytial virus (RSV) is one of the most prevalent causative agents of lower respiratory tract infections worldwide, especially in infants around 3 to 4months old. Infants at such a young age have maternally-transferred passive antibodies against RSV but do not have active immune systems efficient enough for the control of RSV infection. In order to elucidate age-specific profiles of immune responses against RSV protection, antibody responses were examined by using blood samples in both acute and convalescent phases obtained from child patients and adult patients...
February 2017: EBioMedicine
https://www.readbyqxmd.com/read/28102026/piperine-a-pungent-alkaloid-from-black-pepper-inhibits-b-lymphocyte-activation-and-effector-functions
#14
David A Soutar, Carolyn D Doucette, Robert S Liwski, David W Hoskin
Piperine has several well-documented anti-inflammatory properties; however, little is known regarding its effect on humoral immunity. In this study, we describe the immunosuppressive effect of piperine on B lymphocytes, which are integral to the humoral immune response. Mouse B cells were cultured in the absence or presence of non-cytotoxic concentrations (25, 50, and 100 μM) of piperine during T-dependent or T-independent stimulation. Piperine inhibited B cell proliferation by causing G0/G1 phase cell cycle arrest in association with reduced expression of cyclin D2 and D3...
January 19, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28091579/impact-of-age-and-vaccination-history-on-long-term-serological-responses-after-symptomatic-b-pertussis-infection-a-high-dimensional-data-analysis
#15
Inonge van Twillert, Axel A Bonačić Marinović, Betsy Kuipers, Jacqueline A M van Gaans-van den Brink, Elisabeth A M Sanders, Cécile A C M van Els
Capturing the complexity and waning patterns of co-occurring immunoglobulin (Ig) responses after clinical B. pertussis infection may help understand how the human host gradually loses protection against whooping cough. We applied bi-exponential modelling to characterise and compare B. pertussis specific serological dynamics in a comprehensive database of IgG, IgG subclass and IgA responses to Ptx, FHA, Prn, Fim2/3 and OMV antigens of (ex-) symptomatic pertussis cases across all age groups. The decay model revealed that antigen type and age group were major factors determining differences in levels and kinetics of Ig (sub) classes...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090683/similar-but-not-consistent-revisiting-the-pitfalls-of-measuring-igg-subclasses-with-different-assays
#16
Beatrice Ludwig-Kraus, Frank Bernhard Kraus
BACKGROUND: Laboratory quantification of IgG subclasses (IgGSc) is a well-established second-line tool for differential diagnosis of immune deficiencies. However, so far there is still no internationally approved standard available for IgGSc, and different assays are prone to produce divergent results. In this study, we evaluated the comparability and equivalence of two commercially available IgGSc assays, one being the Siemens IgGSc assay on a BN ProSpec analyzer and the other being The Binding Site (TBS) IgGSc assay on a Roche cobas c502 analyzer...
January 16, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28078662/low-immunoglobulin-e-flags-two-distinct-types-of-immune-dysregulation
#17
M Elkuch, V Greiff, C T Berger, M Bouchenaki, T Daikeler, A Bircher, A A Navarini, I Heijnen, M Recher
During the last two decades, hyper-immunoglobulin (Ig)E syndromes have been characterized clinically and molecularly in patients with genetically determined primary immunodeficiencies. However, the detection of low IgE levels, defined here as below detection limit in the routine clinical immunology laboratory, has received little attention. We analysed the association of serum IgA, IgM and IgG levels (including IgG subclasses) with low, normal or high serum IgE levels in patients evaluated in a single-centre out-patient immunodeficiency and allergy clinic...
March 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28075502/antibody-and-cytokine-levels-in-humans-fed-on-by-the-common-bed-bug-cimex-lectularius-l
#18
Johnathan M Sheele, Gale E Ridge, Katirina Coppolino, Tracey Bonfield, Andrew B Young, Stephanie L Gaines, Thomas S McCormick
Little is known about cimicosis, the resultant dermal reaction from feeding activity by the common bed bug, Cimex lectularius L. We fed C. lectularius on human study subjects four times over four weeks, measured serum cytokine and antibody levels, and subjects recorded any cimicosis. The average time for subjects to develop cimicosis decreased with each feeding from 8.4, to 2.1, 1.5, and 1.3 days, respectively. There were no significant changes in total IgG, IgG1, IgG2, IgG4, or IgE levels between the first and fourth bed bug feedings, but there was a significant decrease in total IgG3 levels (p < 0...
January 11, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28065739/r-loop-depletion-by-over-expressed-rnase-h1-in-mouse-b-cells-increases-activation-induced-deaminase-access-to-the-transcribed-strand-without-altering-frequency-of-isotype-switching
#19
Robert W Maul, Hyongi Chon, Kiran Sakhuja, Susana M Cerritelli, Lina A Gugliotti, Patricia J Gearhart, Robert J Crouch
R-loops, three-strand structures consisting of mRNA hybridized to the complementary DNA and a single-stranded DNA loop, are formed in switch regions on the heavy-chain immunoglobulin locus. To determine if R-loops have a direct effect on any of the steps involved in isotype switching, we generated a transgenic mouse that over-expressed RNase H1, an enzyme that cleaves the RNA of RNA/DNA hybrids in B cells. R-loops in the switch μ region were depleted by 70% in ex vivo activated splenic B cells. Frequencies of isotype switching to IgG1, IgG2b, IgG2c, and IgG3 were the same as C57BL/6 control cells...
January 6, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28062698/monoclonal-antibodies-against-epidermal-growth-factor-receptor-acquire-an-ability-to-kill-tumor-cells-through-complement-activation-by-mutations-that-selectively-facilitate-the-hexamerization-of-igg-on-opsonized-cells
#20
Annalina Tammen, Stefanie Derer, Ralf Schwanbeck, Thies Rösner, Anna Kretschmer, Frank J Beurskens, Janine Schuurman, Paul W H I Parren, Thomas Valerius
Triggering of the complement cascade induces tumor cell lysis via complement-dependent cytotoxicity (CDC) and attracts and activates cytotoxic cells. It therefore represents an attractive mechanism for mAb in cancer immunotherapy development. The classical complement pathway is initiated by IgG molecules that have assembled into ordered hexamers after binding their Ag on the tumor cell surface. The requirements for CDC are further impacted by factors such as Ab epitope, valency, and affinity. Thus, mAb against well-validated solid tumor targets, such as the epidermal growth factor receptor (EGFR) that effectively induces complement activation and CDC, are highly sought after...
January 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
keyword
keyword
111035
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"