Edward Portal, Ghislaine Descours, Christophe Ginevra, Massimo Mentasti, Baharak Afshar, Meera Chand, Jessica Day, Fedoua Echahidi, Laura Franzin, Valeria Gaia, Christian Lück, Alaeddine Meghraoui, Jacob Moran-Gilad, Maria Luisa Ricci, Gerard Lina, Søren Uldum, Jonas Winchell, Robin Howe, Kathryn Bernard, Owen B Spiller, Victoria J Chalker, Sophie Jarraud
Legionella pneumophila, a Gram-negative bacillus, is the causative agent of Legionnaire's disease, a form of severe community-acquired pneumonia. Infection can have high morbidity, with a high proportion of patients requiring ICU admission, and up to 10% mortality, which is exacerbated by the lack of efficacy of typical empirical antibiotic therapy against Legionella spp. The fastidious nature of the entire Legionellaceae family historically required inclusion of activated charcoal in the solid medium to remove growth inhibitors, which inherently interferes with accurate antimicrobial susceptibility determination, an acknowledged methodological shortfall, now rectified by a new solid medium that gives results comparable to those of microbroth dilution...
April 13, 2021: Journal of Antimicrobial Chemotherapy