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https://www.readbyqxmd.com/read/27659695/development-of-an-indicator-displacement-based-detection-of-malaria-targeting-hrp-ii-as-biomarker-for-application-in-point-of-care-settings
#1
Babina Chakma, Priyamvada Jain, Naveen K Singh, Pranab Goswami
A novel label free spectrophotometric detection of malarial biomarker HRP-II following an indicator displacement assay has been developed. The assay is based on competitive displacement of murexide dye from its complex with Ni(2+) by HRP-II present in serum samples. The binding constant (Kd) discerned for the dye and HRP-II to Ni(2+) were 1.4 × 10(-6) M(-1) and 6.8 × 10(-9) M(-1), respectively. The progress of the reaction could be monitored from the change of color from orange (∼λ482 nm) to pink (∼λ515 nm) with the concomitant increase in HRP-II concentration in the mixture...
October 18, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27621696/the-quality-characteristics-of-salted-ground-pork-patties-containing-various-fat-levels-by-microwave-cooking
#2
Jong Youn Jeong, Seung Taek Lim, Cheon Jei Kim
This study was carried out to evaluate the effects of fat level on the microwave cooking properties of ground pork patties with NaCl (1.5%). Ground pork patties were processed from pork hams to achieve fat levels of 10%, 15%, 20%, and 25%, respectively. Each patty was cooked from a thawed state to 75℃ in a microwave oven at full power (700 W). After microwave cooking, protein content, moisture content, fat retention, and shear force values in patties decreased as fat level increased from 10 to 25%. As fat level increased, cooking time decreased but total cooking loss and drip loss were increased, whereas slight differences in diameter reduction and thickness of patties were observed...
2016: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/27562780/halopiger-thermotolerans-sp-nov-a-thermo-tolerant-haloarchaeon-isolated-from-commercial-salt
#3
Hiroaki Minegishi, Ryuta Shimogaki, Shigeaki Enomoto, Akinobu Echigo, Yusuke Kondo, Shuhei Nagaoka, Yasuhiro Shimane, Masahiro Kamekura, Takashi Itoh, Moriya Ohkuma, Takuro Nunoura, Ken Takai, Ron Usami
Three thermo-tolerant halophilic archaeal strains, SR-441T, SR-412 and SR-188 were isolated from commercial salt samples. Cells were non-motile pleomorphic rod-shaped, and stained Gram-negative. Colonies were pink-pigmented. The three strains were able to grow at 1.7-4.6 M NaCl (optimum, 2.5 M) at pH 6.5-9.0 (optimum, pH 8.0) and at 35-60 °C (optimum, 45 °C). The orthologous 16S rRNA gene sequence similarities amongst three strains were 98.8-99.3 %, and the level of DNA-DNA relatedness was 71-74 and 72-75 % (reciprocally)...
August 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27189380/multispectroscopic-and-isotopic-ratio-analysis-to-characterize-the-inorganic-binder-used-on-pompeian-pink-and-purple-lake-pigments
#4
Iker Marcaida, Maite Maguregui, Héctor Morillas, Cristina García-Florentino, Ulla Knuutinen, Jose Antonio Carrero, Silvia Fdez-Ortiz de Vallejuelo, Africa Pitarch Martı́, Kepa Castro, Juan Manuel Madariaga
Because of the fact that pigments are not ubiquitous in the archeological record, the application of noninvasive analytical methods is a necessity. In this work, pink and purple lake pigments recovered from the excavations of the ancient city of Pompeii (Campania, Italy) and preserved in their original bowls at the Naples National Archaeological Museum (Italy) were analyzed to characterize the composition of their inorganic binders (mordants). In situ preliminary analyses using a hand-held energy dispersive X-ray fluorescence spectrometer (HH-ED-XRF) allowed us to determine the use of an aluminosilicate enriched in Cu and Pb...
June 21, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/26394634/overview-and-assessment-of-the-histochemical-methods-and-reagents-for-the-detection-of-%C3%AE-galactosidase-activity-in-transgenic-animals
#5
Stefan Trifonov, Yuji Yamashita, Masahiko Kase, Masato Maruyama, Tetsuo Sugimoto
Bacterial β-galactosidase is one of the most widely used reporter genes in experiments involving transgenic and knockout animals. In this review we discuss the current histochemical methods and available reagents to detect β-galactosidase activity. Different substrates are available, but the most commonly used is X-gal in combination with potassium ferri- and ferro-cyanide. The reaction produces a characteristic blue precipitate in the cells expressing β-galactosidase, and despite its efficiency in staining whole embryos, its detection on thin tissue sections is difficult...
January 2016: Anatomical Science International
https://www.readbyqxmd.com/read/26246266/halophilic-microbial-communities-in-deteriorated-buildings
#6
REVIEW
Justyna Adamiak, Anna Otlewska, Beata Gutarowska
Halophilic microorganisms were traditionally isolated from an aquatic environment. There has been little research conducted into halophiles inhabiting the terrestrial environment in which historic monuments deteriorate. Salt efflorescence deposited on the walls is an observed phenomenon on the surface of historic buildings, and would favour the growth of halophiles. However, some conditions have to be fulfilled in order for efflorescence to occur: (1) the presence of salts, (2) porosity, (3) a source of water...
October 2015: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/26207611/electrostatic-and-allosteric-cooperativity-in-ion-pair-binding-a-quantitative-and-coupled-experiment-theory-study-with-aryl-triazole-ether-macrocycles
#7
Bo Qiao, Arkajyoti Sengupta, Yun Liu, Kevin P McDonald, Maren Pink, Joseph R Anderson, Krishnan Raghavachari, Amar H Flood
Cooperative binding of ion pairs to receptors is crucial for the manipulation of salts, but a comprehensive understanding of cooperativity has been elusive. To this end, we combine experiment and theory to quantify ion-pair binding and to separate allostery from electrostatics to understand their relative contributions. We designed aryl-triazole-ether macrocycles (MC) to be semiflexible, which allows ion pairs (NaX; X = anion) to make contact, and to be monocyclic to simplify analyses. A multiequilibrium model allows us to quantify, for the first time, the experimental cooperativity, α, for the equilibrium MC·Na(+) + MC·X(-) ⇌ MC·NaX + MC, which is associated with contact ion-pair binding of NaI (α = 1300, ΔGα = -18 kJ mol(-1)) and NaClO4 (α = 400, ΔGα = -15 kJ mol(-1)) in 4:1 dichloromethane-acetonitrile...
August 5, 2015: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/26003439/development-of-selective-and-differential-medium-for-shigella-sonnei-using-three-carbohydrates-lactose-sorbitol-and-xylose-and-x-gal
#8
G N Na, S A Kim, O C Kwon, M S Rhee
The aim of this study was to develop a new selective and differential medium for isolating Shigella sonnei (designated 3SD medium). The new medium was based on three carbohydrates (lactose, sorbitol, and xylose) and a chromogenic substrate (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, X-Gal). S. sonnei cannot ferment lactose, sorbitol, or xylose, but can ferment X-Gal, which generates turquoise-blue colonies with rough edges. Other bacteria (54 strains of foodborne pathogens and spoilage bacteria) produced visually distinct colonies on 3SD medium (colorless or pink-violet colonies), or their growth was inhibited on 3SD medium...
August 2015: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/25800527/possible-correlation-between-bile-salt-hydrolysis-and-ahl-deamidation-staphylococcus-epidermidis-rm1-a-potent-quorum-quencher-and-bile-salt-hydrolase-producer
#9
Ruchira Mukherji, Asmita Prabhune
The aim of the present work was to isolate a bile salt hydrolase (BSH) producer from fermented soy curd and explore the ability of the BSH produced to cleave bacterial quorum sensing signals. Bacterial isolates with possible ability to deconjugate bile salts were enriched and isolated on De Man, Rogosa and Sharpe (MRS) medium containing 0.2% bile salts. BSH-producing positive isolate with orange-pink-pigmented colonies was isolated and was identified as a strain of Staphylococcus epidermidis using biochemical and phylogenetic tools...
May 2015: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/25744586/halorubrum-persicum-sp-nov-an-extremely-halophilic-archaeon-isolated-from-sediment-of-a-hypersaline-lake
#10
Paulina Corral, Rafael R de la Haba, Cristina Sánchez-Porro, Mohammad Ali Amoozegar, R Thane Papke, Antonio Ventosa
An extremely halophilic archaeon belonging to the genus Halorubrum, strain C49T, was isolated from sediment of the hypersaline lake Aran-Bidgol in Iran. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain C49T was closely related to Halorubrum saccharovorum JCM 8865T (99.5 %) and other species of the genus Halorubrum. Studies based on multilocus sequence analysis revealed that strain C49T is placed among the species of Halorubrum; the strain constituted a defined branch in comparison with the type strains of species of Halorubrum, while the 16S rRNA gene sequence divergence could not define the status of the newly isolated strain...
June 2015: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/25721723/halococcus-agarilyticus-sp-nov-an-agar-degrading-haloarchaeon-isolated-from-commercial-salt
#11
Hiroaki Minegishi, Akinobu Echigo, Yasuhiro Shimane, Masahiro Kamekura, Takashi Itoh, Moriya Ohkuma, Ron Usami
Two agar-degrading halophilic archaeal strains, 62 E(T) and 197 A, were isolated from commercial salt samples. Cells were non-motile cocci, approximately 1.2-2.0 µm in diameter and stained Gram-negative. Colonies were pink-pigmented. Strain 62 E(T) was able to grow with 24-30% (w/v) NaCl (optimum, 27%), at pH 6.5-8.5 (optimum, pH 7.5) and at 22-47 °C (optimum, 42 °C). The 16S rRNA gene sequences of strains 62 E(T) and 197 A were identical, and the level of DNA-DNA relatedness between them was 90 and 90% (reciprocally)...
May 2015: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/25479949/halostagnicola-bangensis-sp-nov-an-alkaliphilic-haloarchaeon-from-a-soda-lake
#12
Paulina Corral, Angela Corcelli, Antonio Ventosa
An extremely haloalkaphilic archaeon, strain T26(T), belonging to the genus Halostagnicola, was isolated from sediment of the soda lake Bange in the region of Tibet, China. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain T26(T) was closely related to Halostagnicola alkaliphila 167-74(T) (98.4 %), Halostagnicola larsenii XH-48(T) (97.5 %) and Halostagnicola kamekurae 194-10(T) (96.8 %). Strain T26(T) grew optimally in media containing 25 % (w/v) salts, at pH 9.0 and 37 °C in aerobic conditions...
March 2015: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/25367341/halorubrum-laminariae-sp-nov-isolated-from-the-brine-of-salted-brown-alga-laminaria
#13
Dong Han, Heng-Lin Cui
Two halophilic archaeal strains, R60(T) and R61, were isolated from the brine of salted brown alga Laminaria. Cells of the two strains were observed to be rod-shaped, stain Gram-negative and to lyse in distilled water. Strain R60(T) was found to contain gas vacuoles and to produce pink-pigmented colonies, while strain R61 lacked gas vacuoles and produces red-pigmented colonies. Both strains were found to be able to grow at 20-50 °C (optimum 30 °C), at 1.7-4.8 M NaCl (optimum 2.6-3.1 M NaCl), at 0-1.0 M MgCl2 (optimum 0...
January 2015: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/25319616/colorimetric-detection-of-in-situ-metal-acetates-and-fluorides-by-a-bipyridyl-linked-schiff-base
#14
Sivalingam Suganya, Hye Jin Zo, Jong S Park, Sivan Velmathi
Here, we present a new bipyridyl moiety linked Schiff base (bipy-1) that is well characterized using spectroscopic techniques. Colorimetric and UV-vis titrations were used to study the photophysical properties of bipy-1 in the presence of various tetrabutyl ammonium salt of anions and metal salts containing different counter cations. bipy-1 showed selective recognition of dimethyl sulphoxide solution of tetrabutyl ammonium salt of F(-) ion accompanied with a UV-vis band at 529 nm and interesting binding of aqueous Co, Ni, and Cu acetates/fluorides, as confirmed by distinct color changes from fluorescent green to pink or orange and a strong band around 480-510 nm in the UV-vis spectrum...
December 2014: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/25310808/efficacy-of-supplementation-of-selected-medicinal-mushrooms-with-inorganic-selenium-salts
#15
Przemysław Niedzielski, Mirosław Mleczek, Marek Siwulski, Monika Gąsecka, Lidia Kozak, Iwona Rissmann, Patrycja Mikołajczak
The aim of the study was to evaluate the possibility of supplementation with inorganic forms of selenium (Na2SeO4 and Na2SeO3) in concentrations of 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 and 1.5 mM of three medicinal mushroom species: Agrocybe aegerita, Hericium erinaceus and Ganoderma lucidum. Tested mushroom species grew in Se additions of 0-0.6 mM (A. aegerita and H. erinaceus), while growth of G. lucidum bodies was observed for 0-0.8 mM. For the latter mushroom species, the total Se content was the highest...
2014: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/25019418/validation-of-caffeine-dehydrogenase-from-pseudomonas-sp-strain-cbb1-as-a-suitable-enzyme-for-a-rapid-caffeine-detection-and-potential-diagnostic-test
#16
Sujit K Mohanty, Chi Li Yu, Sridhar Gopishetty, Mani Subramanian
Excess consumption of caffeine (>400 mg/day/adult) can lead to adverse health effects. Recent introduction of caffeinated products (gums, jelly beans, energy drinks) might lead to excessive consumption, especially among children and nursing mothers, hence attracting the Food and Drug Administration's attention and product withdrawals. An "in-home" test will aid vigilant consumers in detecting caffeine in beverages and milk easily and quickly, thereby restricting its consumption. Known diagnostic methods lack speed and sensitivity...
August 6, 2014: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/24863363/halophilic-bacteria-are-colonizing-the-exhibition-areas-of-the-capuchin-catacombs-in-palermo-italy
#17
G Piñar, L Kraková, D Pangallo, D Piombino-Mascali, F Maixner, A Zink, K Sterflinger
The Capuchin Catacombs of Palermo, Italy, contain over 1800 mummies dating from the 16th to 20th centuries AD. Their environment is not conducive to the conservation of the remains due to, among other factors, water infiltration, which is producing salt efflorescences on the walls. A multiphasic approach was applied to investigate the halophilic microbiota present in the Catacombs. Enrichment cultures were conducted on media containing different NaCl concentrations, ranging from 3 to 20 %. For screening of the strains, the following two PCR-based methods were used and compared: fluorescence internal transcribed spacer PCR (f-ITS) and random amplification of polymorphic DNA (RAPD) analyses...
July 2014: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/24862325/effects-of-dry-brining-liquid-smoking-and-high-pressure-treatment-on-the-physical-properties-of-aquacultured-king-salmon-oncorhynchus-tshawytscha-during-refrigerated-storage
#18
COMPARATIVE STUDY
Kelvin Jia Wey Kong, Zayde Alçiçek, Murat O Balaban
BACKGROUND: Aquacultured King salmon (Oncorhynchus tshawytscha) pieces were dry brined with a salt/brown sugar mix, dipped in liquid smoke for 3 min, vacuum packed, high hydrostatic pressure (HHP) treated at 600 or 200 MPa for 5 min and stored at 4 °C for up to 40 days. RESULTS: The surface redness (average a*) of the samples increased after dry brining, then decreased after liquid smoke treatment. HHP did not change the outside color of liquid-smoked samples. However, the inside color changed depending on pressure...
March 15, 2015: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/24857419/a-nitrido-salt-reagent-of-titanium
#19
Rick Thompson, Chun-Hsing Chen, Maren Pink, Gang Wu, Daniel J Mindiola
Deprotonation of the parent titanium imido ((tBu)nacnac)Ti≡NH(Ntolyl2) ((tBu)nacnac(-) = [ArNC(t)Bu]2CH; Ar = 2,6-(i)Pr2C6H3) with KCH2Ph forms a rare example of a molecular titanium nitride as a dimer, {[K][((tBu)nacnac)Ti≡N(Ntolyl2)]}2. From the parent imido or nitride salt, the corresponding aluminylimido-etherate adduct, ((tBu)nacnac)Ti≡N[AlMe2(OEt2)](Ntolyl2), can be isolated and structurally characterized. The parent imido is also a source for the related borylimido, ((tBu)nacnac)Ti═NBEt2(Ntolyl2)...
June 11, 2014: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/24598995/alleviation-of-salt-stress-by-enterobacter-sp-ej01-in-tomato-and-arabidopsis-is-accompanied-by-up-regulation-of-conserved-salinity-responsive-factors-in-plants
#20
Kangmin Kim, Ye-Jin Jang, Sang-Myeong Lee, Byung-Taek Oh, Jong-Chan Chae, Kui-Jae Lee
Microbiota in the niches of the rhizosphere zones can affect plant growth and responses to environmental stress conditions via mutualistic interactions with host plants. Specifically, some beneficial bacteria, collectively referred to as Plant Growth Promoting Rhizobacteria (PGPRs), increase plant biomass and innate immunity potential. Here, we report that Enterobacter sp. EJ01, a bacterium isolated from sea china pink (Dianthus japonicus thunb) in reclaimed land of Gyehwa-do in Korea, improved the vegetative growth and alleviated salt stress in tomato and Arabidopsis...
February 2014: Molecules and Cells
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