keyword
MENU ▼
Read by QxMD icon Read
search

Non viral vectors

keyword
https://www.readbyqxmd.com/read/29447156/west-nile-and-st-louis-encephalitis-viral-genetic-determinants-of-avian-host-competence
#1
Payal D Maharaj, Angela M Bosco-Lauth, Stanley A Langevin, Michael Anishchenko, Richard A Bowen, William K Reisen, Aaron C Brault
West Nile virus (WNV) and St. Louis encephalitis (SLEV) virus are enzootically maintained in North America in cycles involving the same mosquito vectors and similar avian hosts. However, these viruses exhibit dissimilar viremia and virulence phenotypes in birds: WNV is associated with high magnitude viremias that can result in mortality in certain species such as American crows (AMCRs, Corvus brachyrhynchos) whereas SLEV infection yields lower viremias that have not been associated with avian mortality. Cross-neutralization of these viruses in avian sera has been proposed to explain the reduced circulation of SLEV since the introduction of WNV in North America; however, in 2015, both viruses were the etiologic agents of concurrent human encephalitis outbreaks in Arizona, indicating the need to re-evaluate host factors and cross-neutralization responses as factors potentially affecting viral co-circulation...
February 15, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29442899/highly-efficient-glioma-targeting-of-tat-peptide-tta1-aptamer-polyephylene-glycol-modified-gelatin-siloxane-nanoparticles
#2
Xiao-Ning Lin, Xinli Tian, Wang Li, Jin Sun, Feng Wei, Wei Feng, Zhi-Chun Huang, Xin-Hua Tian
Gliomas are the most common type of intracranial malignant tumor; however, current treatment approaches are often ineffective due to limited penetration of genes or drugs through the blood-brain barrier (BBB). Here we describe the synthesis of gelatin-siloxane nanoparticles (GS NPs) as candidate gene carriers through a two-step sol-gel process. To increase the efficiency of glioma targeting, human immunodeficiency virus-derived Tat, tumor-targeting aptamer (TTA)1, and polyethylene glycol (PEG) were conjugated to the GS NPs to generate Tat-TTA1-PEG-GS NPs...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442622/chitosan-stabilized-gold-folate-poly-lactide-co-glycolide-nanoplexes-facilitate-efficient-gene-delivery-in-hepatic-and-breast-cancer-cells
#3
Jude Akinyelu, Moganavelli Singh
The biodegradable polymer, poly(lactide-co-glycolide) is a popular polymer of choice in many nanotherapeutic studies. Herein, we report on the synthesis and evaluation of four chitosan stabilized poly(lactide-co-glycolide) nanoparticles with and without coating with gold, and the targeting ligand, folic acid, as potential non-viral gene delivery vectors. The poly(lactide-co-glycolide) nanoparticles were synthesized via nanoprecipitation/solvent evaporation method in conjunction with the surface functionalizing folic acid and chitosan...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29429122/optimal-control-approach-for-establishing-wmelpop-wolbachia-infection-among-wild-aedes-aegypti-populations
#4
Doris E Campo-Duarte, Olga Vasilieva, Daiver Cardona-Salgado, Mikhail Svinin
Wolbachia-based biocontrol has recently emerged as a potential method for prevention and control of dengue and other vector-borne diseases. Major vector species, such as Aedes aegypti females, when deliberately infected with Wolbachia become less capable of getting viral infections and transmitting the virus to human hosts. In this paper, we propose an explicit sex-structured population model that describes an interaction of uninfected (wild) male and female mosquitoes and those deliberately infected with wMelPop strain of Wolbachia in the same locality...
February 10, 2018: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/29427436/highly-metastatic-k7m2-cell-line-a-novel-murine-model-capable-of-in-vivo-imaging-via-luciferase-vector-transfection
#5
Brian T Grisez, Justin J Ray, Phillip A Bostian, Justin E Markel, Brock A Lindsey
Osteosarcoma is rare and little improvement in survival rates has occurred in the last 25 years despite modern chemotherapeutic treatment. Bioluminescent cell lines for the modeling of osteosarcoma have shown success in tracking metastases in vivo, but commonly use adenoviral vectors to transfect the native cell line with bioluminescent reporters. The purpose of this study was to develop an orthotopic model for metastatic osteosarcoma capable of in vivo monitoring of metastatic and primary tumor burden in an immunocompetent mouse and compare that model to its wild type pathogenesis...
February 10, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29427115/gene-therapies-for-polyglutamine-diseases
#6
Carlos A Matos, Vítor Carmona, Udaya-Geetha Vijayakumar, Sara Lopes, Patrícia Albuquerque, Mariana Conceição, Rui Jorge Nobre, Clévio Nóbrega, Luís Pereira de Almeida
Polyglutamine diseases are hereditary degenerative disorders of the nervous system that have remained, to this date, untreatable. Promisingly, investigation into their molecular etiology and the development of increasingly perfected tools have contributed to the design of novel strategies with therapeutic potential. Encouraging studies have explored gene therapy as a means to counteract cell demise and loss in this context. The current chapter addresses the two main focuses of research in the area: the characteristics of the systems used to deliver nucleic acids to cells and the molecular and cellular actions of the therapeutic agents...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29423922/polymer-coated-viral-vectors-hybrid-nanosystems-for-gene-therapy
#7
REVIEW
Pratheppa Rajagopal, Sowmiya Duraiswamy, Swaminathan Sethuraman, Giridhara R Jayandharan, Uma Maheswari Krishnan
AIM: The advantages and critical aspects of nano-dimensional polymer-coated viral vector systems potentially applicable for gene delivery are reviewed in this article. DISCUSSION: Various viral and non-viral vectors have been explored for gene therapy. Viral gene transfer methods though highly efficient, are limited by their immunogenicity. Non-viral vectors have lower transfection efficiency due to their inability to escape from the endosome. To overcome these drawbacks, novel nanotechnology-mediated interventions that involve coating or modification of virus using polymers have emerged as a new paradigm in gene therapy...
February 8, 2018: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29422377/icon-immunoconjugate-treatment-results-in-regression-of-red-lesions-in-a-non-human-primate-papio-anubis-model-of-endometriosis
#8
Demetra Hufnagel, Laura G Goetz, Zhiwei Hu, Atunga Nyachieo, Thomas D'Hooghe, Asgerally Fazleabas, Antoni Duleba, Graciela Krikun, Hugh S Taylor, Charles J Lockwood
Endometriosis is a common condition in reproductive-aged women characterized by ectopic endometrial lesions of varied appearance, including red, white, blue, black or powder burn coloration, which contribute to chronic pain and infertility. The immunoconjugate molecule (Icon) targets Tissue Factor, a transmembrane receptor for Factor VII/VIIa that is aberrantly expressed in the endothelium supporting ectopic endometrial tissue. Icon has been shown to cause regression of endometriosis in a murine model of disease but prior to this study had not been tested in non-human primates...
February 5, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29415514/covalent-strategies-for-targeting-messenger-and-non-coding-rnas-an-updated-review-on-sirna-mirna-and-antimir-conjugates
#9
REVIEW
Santiago Grijalvo, Adele Alagia, Andreia F Jorge, Ramon Eritja
Oligonucleotide-based therapy has become an alternative to classical approaches in the search of novel therapeutics involving gene-related diseases. Several mechanisms have been described in which demonstrate the pivotal role of oligonucleotide for modulating gene expression. Antisense oligonucleotides (ASOs) and more recently siRNAs and miRNAs have made important contributions either in reducing aberrant protein levels by sequence-specific targeting messenger RNAs (mRNAs) or restoring the anomalous levels of non-coding RNAs (ncRNAs) that are involved in a good number of diseases including cancer...
February 6, 2018: Genes
https://www.readbyqxmd.com/read/29413593/therapeutic-strategies-for-flexor-tendon-healing-by-nanoparticle-mediated-co-delivery-of-bfgf-and-vegfa-genes
#10
Qian Qian Yang, Yi Xiang Shao, Lu Zhong Zhang, You Lang Zhou
Tendon injuries are a common injury of musculocutaneous system. Due to the lack of sufficient cellularity and low growth factor activity, healing of disrupted digital flexor tendon is troublesome and the process is lengthy and ineffective. bFGF and VEGFA gene were proved to be responsible and critical for promoting tendon healing. How to continuously enhance expression of these genes is a challenge. In this study, we developed a combination therapeutic approach that corrects the fundamental problem underlying intrasynovial tendon healing with introduction of growth factor genes via non-viral vector nanoparticle...
January 27, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29408269/aedes-aegypti-antiviral-adaptive-response-against-denv-2
#11
Javier Serrato-Salas, Javier Izquierdo-Sánchez, Martha Argüello, Renáud Conde, Alejandro Alvarado-Delgado, Humberto Lanz-Mendoza
Priming is the conceptual term defining memory phenomenon in innate immune response. Numerous examples of enhanced secondary immune response have been described in diverse taxa of invertebrates; which naturally lacks memory response. In mosquitoes, a previous non-lethal challenge with some specific pathogens modify their immune response against the same microorganism; developing an improved antimicrobial reaction. In this work, we explore the ability of Aedes aegypti to mount a higher antiviral response upon a second oral DENV challenge...
February 2, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29407238/outbreak-of-zika-virus-pathogenesis-and-quest-of-its-vaccine-development-where-do-we-stand-now
#12
REVIEW
Ameer Khusro, Chirom Aarti, Alberto Barbabosa-Pilego, Pedro Sanchez-Aparicio
Zika virus (ZIKV) infection is a life-threatening tropical infection, mainly caused by mosquito bite. After a very long period of quietness, ZIKV infections have become a problematic issue again. Previously, the virus was limited to Africa and Asia only but later it emerged in Brazil, South America, and other parts of the world in 2015. In 2016, there are emerging new cases of sexually transmitted ZIKV infection as well. At present, there is no proper treatment and available pronounced vaccines for the treatment of ZIKV infection...
February 3, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29395384/the-polyplex-protein-corona-cell-interplay-tips-and-drawbacks
#13
Daniele Maiolo, Jessica Colombo, Jennifer Beretta, Chiara Malloggi, Gabriele Candiani, Francesca Baldelli Bombelli
Polyplexes (PX) are soft materials, obtained by blending polycations and nucleic acids, designed for gene delivery applications. While much is known about the transfection properties of PX, their protein corona, the biomolecules interacting with colloids once in a biological environment, represents an underlooked parameter in gene transfection. In this study, linear and branched polyethylenimines (lPEI and bPEI), the golden standard among non-viral vectors, were selected and used throughout the work: their physicochemical properties and protein corona when complexed to DNA were studied and linked to the toxicity and transfection efficiency arisen upon their delivery to cells...
January 30, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29373809/pressing-diseases-that-represent-promising-targets-for-gene-therapy
#14
Marie Beitelshees, Andrew Hill, Pooya Rostami, Charles H Jones, Blaine A Pfeifer
Over time, there has been a growing interest in the application of gene therapy within the healthcare industry as demonstrated by the nearly 3,000 clinical trials associated with gene therapy that are listed in clinicaltrials.gov. However, there are various difficulties associated with gene therapy that have limited the realization of licensed gene therapies to only a handful of treatments. Furthermore, efforts to develop gene therapeutics have been narrowly focused and most clinical trials have sought to develop treatments for cancer (64...
December 2017: Discovery Medicine
https://www.readbyqxmd.com/read/29367685/in-vivo-imaging-of-adeno-associated-viral-vector-labelled-retinal-ganglion-cells
#15
Corey A Smith, Balwantray C Chauhan
A defining characteristic of optic neuropathies, such as glaucoma, is progressive loss of retinal ganglion cells (RGCs). Current clinical tests only provide weak surrogates of RGC loss, but the possibility of optically visualizing RGCs and quantifying their rate of loss could represent a radical advance in the management of optic neuropathies. In this study we injected two different adeno-associated viral (AAV) vector serotypes in the vitreous to enable green fluorescent protein (GFP) labelling of RGCs in wild-type mice for in vivo and non-invasive imaging...
January 24, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29364270/an-efficient-in-vitro-transposition-method-by-a-transcriptionally-regulated-sleeping-beauty-system-packaged-into-an-integration-defective-lentiviral-vector
#16
Daniela Benati, Fabienne Cocchiarella, Alessandra Recchia
The Sleeping Beauty (SB) transposon is a non-viral integrating system with proven efficacy for gene transfer and functional genomics. To optimize the SB transposon machinery, a transcriptionally regulated hyperactive transposase (SB100X) and T2-based transposon are employed. Typically, the transposase and transposon are provided transiently by plasmid transfection and SB100X expression is driven by a constitutive promoter. Here, we describe an efficient method to deliver the SB components to human cells that are resistant to several physical and chemical transfection methods, to control SB100X expression and stably integrate a gene of interest (GOI) through a "cut and paste" SB mechanism...
January 12, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29353669/transmission-of-dengue-virus-from-deceased-donors-to-solid-organ-transplant-recipients-case-report-and-literature-review
#17
Fernando Rosso, Juan C Pineda, Ana M Sanz, Jorge A Cedano, Luis A Caicedo
Dengue fever is a vector-transmitted viral infection. Non-vectorial forms of transmission can occur through organ transplantation. We reviewed medical records of donors and recipients with suspected dengue in the first post-transplant week. We used serologic and molecular analysis to confirm the infection. Herein, we describe four cases of dengue virus transmission through solid organ transplantation. The recipients had positive serology and RT-PCR. Infection in donors was detected through serology. All cases presented with fever within the first week after transplantation...
January 18, 2018: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29353086/ruthenium-dendrimers-as-carriers-for-anticancer-sirna
#18
Sylwia Michlewska, Maksim Ionov, Marta Maroto-Díaz, Aleksandra Szwed, Aliaksei Ihnatsyeu-Kachan, Svetlana Loznikova, Dzmitry Shcharbin, Marek Maly, Rafael Gomez Ramirez, Francisco Javier de la Mata, Maria Bryszewska
Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence...
January 12, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29352891/efficient-gene-delivery-by-oligochitosan-conjugated-serum-albumin-facile-synthesis-polyplex-stability-and-transfection
#19
Monika Kumari, Chi-Hsien Liu, Wei-Chi Wu
Chitosan and its derivatives have shown to be potential gene carriers with biocompatiblility and safety. However, their practical delivery is far from being ideal because of the low transfection efficiency. The present work describes the potential of a natural protein, bovine serum albumin (BSA), conjugated with a natural oligosaccharide, oligochitosan (OC), as a considerable promising approach for a safe and efficient non-viral gene delivery vector. The FTIR spectra proved the effective conjugation of BSA with OC through covalent bond...
March 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29343574/novel-human-polyomavirus-non-coding-control-regions-differ-in-bi-directional-gene-expression-according-to-host-cell-large-t-antigen-expression-and-clinically-occurring-rearrangements
#20
Elvis T Ajuh, Zongsong Wu, Emma Kraus, Fabian H Weissbach, Tobias Bethge, Rainer Gosert, Nicole Fischer, Hans H Hirsch
Human polyomavirus (HPyV) DNA genomes contain three regions denoted early viral gene region (EVGR) encoding the regulatory T-antigens and one microRNA, late viral gene region (LVGR) encoding the structural Vp capsid proteins, and non-coding control region (NCCR). The NCCR harbours the origin of viral genome replication and bi-directional promoter/enhancer functions governing EVGR- and LVGR-expression on opposite DNA strands. Despite principle similarities, HPyV-NCCRs differ in length, sequence, and architecture...
January 17, 2018: Journal of Virology
keyword
keyword
11098
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"