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https://www.readbyqxmd.com/read/28729783/cytotoxic-evaluation-of-a-new-ceramic-based-root-canal-sealer-on-human-fibroblasts
#1
Sandra Chakar, Sylvie Changotade, Nada Osta, Issam Khalil
OBJECTIVES: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). MATERIALS AND METHODS: Cell viability was assessed through direct and indirect contact between human fibroblasts and sealer. Direct contact was performed at 24 h, whereas the indirect contact was performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28726876/bioceramic-nanocomposite-thiol-acrylate-polyhipe-scaffolds-for-enhanced-osteoblastic-cell-culture-in-3d
#2
Aaron Lee, Caitlin R Langford, Luis M Rodriguez-Lorenzo, Helmut Thissen, Neil R Cameron
Emulsion-templated (polyHIPE) scaffolds for bone tissue engineering were produced by photopolymerisation of a mixture of trimethylolpropane tris(3-mercaptopropionate) and dipentaerythritol penta-/hexa-acrylate in the presence of hydroxyapatite (HA) or strontium-modified hydroxyapatite (SrHA) nanoparticles. Porous and permeable polyHIPE materials were produced regardless of the type or incorporation level of the bioceramic, although higher loadings resulted in a larger average pore diameter. Inclusion of HA and SrHA into the scaffolds was confirmed by EDX-SEM, FTIR and XPS and quantified by thermogravimetry...
July 20, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28714662/enhancing-the-osteogenic-capability-of-core-shell-bilayered-bioceramic-microspheres-with-adjustable-biodegradation
#3
Xiurong Ke, Chen Zhuang, Xianyan Yang, Jia Fu, Sanzhong Xu, Lijun Xie, Zhongru Gou, Juncheng Wang, Lei Zhang, Guojing Yang
This study describes the fabrication and biological evaluation of core-shell bilayered bioceramic microspheres with adjustable compositional distribution via a coaxial bilayer capillary system. Beyond the homogeneous hybrid composites, varying the diameter of capillary nozzles and the composition of the bioceramic slurries makes it easy to create bilayered β-tricalcium phosphate (CaP)/β-calcium silicate (CaSi) microspheres with controllable compositional distribution in the core or shell layer. Primary investigations in vitro revealed that biodegradation could be adjusted by compositional distribution or shell thickness and that poorly soluble CaP located on the shell layer of CaP or CaSi@CaP microspheres was particularly beneficial for mesenchymal stem cell adhesion and growth in the early stage, but the ion release from the CaP@CaSi exhibited a potent stimulating effect on alkaline phosphatase expression of the cells at longer times...
July 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28713016/a-conducive-bioceramic-polymer-composite-biomaterial-for-diabetic-wound-healing
#4
Fang Lv, Jie Wang, Peng Xu, Yiming Han, Hongshi Ma, He Xu, Shijie Chen, Jiang Chang, Qinfei Ke, Mingyao Liu, Zhengfang Yi, Chengtie Wu
Diabetic wound is a common complication of diabetes. Biomaterials offer great promise in inducing tissue regeneration for chronic wound healing. Herein, we reported a conducive Poly (caprolactone) (PCL)/gelatin nanofibrous composite scaffold containing silicate-based bioceramic particles (Nagelschmidtite, NAGEL, Ca7P2Si2O16) for diabetic wound healing. NAGEL bioceramic particles were well distributed in the inner of PCL/gelatin nanofibers via co-electrospinning process and the Si ions maintained a sustained release from the composite scaffolds during the degradation process...
July 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28707315/dissolution-behavior-of-cao-mgo-sio2-based-multiphase-bioceramic-powders-and-effects-of-the-released-ions-on-osteogenesis
#5
Mengjiao Zhang, Xianchun Chen, Ximing Pu, Xiaoming Liao, Zhongbing Huang, Guangfu Yin
In the present study, the dissolution behavior of the CaO-MgO-SiO2 -based multiphase bioceramic powders as well as the effects of released ions on osteogenesis was investigated. In the dissolution process, Ca, Mg and Si ions could be dissolved out from the powders. The incorporation of Mg could slow down the degradation rate of the powders so that to reduce the local concentration of Ca and Si ions. In return, the dissolution of Ca and Si ions leading to the formation of a porous SiO2 -rich layer could enhance the readsorption of free Mg ions in solution and thus down-regulate the concentration of Mg ions...
July 13, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28686244/3d-printed-porous-ceramic-scaffolds-for-bone-tissue-engineering-a-review
#6
REVIEW
Yu Wen, Sun Xun, Meng Haoye, Sun Baichuan, Chen Peng, Liu Xuejian, Zhang Kaihong, Yang Xuan, Peng Jiang, Lu Shibi
This study summarizes the recent research status and development of three-dimensional (3D)-printed porous ceramic scaffolds in bone tissue engineering. Recent literature on 3D-printed porous ceramic scaffolds was reviewed. Compared with traditional processing and manufacturing technologies, 3D-printed porous ceramic scaffolds have obvious advantages, such as enhancement of the controllability of the structure or improvement of the production efficiency. More sophisticated scaffolds were fabricated by 3D printing technology...
July 7, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28684351/effectiveness-of-chitosan-scaffold-in-skin-bone-and-cartilage-healing
#7
REVIEW
Ahmad Oryan, Sonia Sahvieh
Chitosan (CS) is a carbohydrate biopolymer, which has been known as a biological material in promoting the healing process of soft and hard connective tissues. It is biocompatible, biodegradable, bioactive, non-toxic, non-expensive and non-immunogenic, with antibacterial capability. Additionally, the capacity of forming complexes with other anionic biomaterials and molecules offers CS the characteristics to be used in biomedical applications. Therefore, this natural polysaccharide has widely been used as a wound dressing and in bone and cartilage regeneration because of these considerable properties...
July 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28680552/investigations-on-the-effects-of-mouthrinses-on-the-colour-stability-and-surface-roughness-of-different-dental-bioceramics
#8
Koray Soygun, Osman Varol, Ali Ozer, Giray Bolayir
PURPOSE: In this study, three bioceramic materials, [IPS Empress CAD (Ivoclar), IPS e.max CAD (Ivoclar), and Lava Ultimate CAD (3M ESPE)] were treated with three commercial mouthrinses [Listerine, Tantum Verde, and Klorhex]; and changes in colour reflectance and surface roughness values were then quantitatively assessed. MATERIALS AND METHODS: One hundred and twenty ceramic samples, with dimensions of 2 × 12 × 14 mm, were prepared and divided into nine sample groups, except three control samples...
June 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/28631614/3d-printed-bioceramic-scaffolds-with-antibacterial-and-osteogenic-activity
#9
Yongliang Zhang, Dong Zhai, Mengchi Xu, Qingqiang Yao, Huiying Zhu, Jiang Chang, Chengtie Wu
Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Silver (Ag) nanoparticles were uniformly dispersed on graphene oxide (GO) to form a homogeneous nanocomposite (named Ag@GO) with different Ag-to-graphene oxide mass ratios, with this being synthesized via the liquid chemical reduction approach...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28629069/evaluation-of-electrospun-biomimetic-substrate-surface-decorated-with-nanohydroxyapatite-precipitation-for-osteoblasts-behavior
#10
Sai Zhang, Guojun Jiang, Molamma P Prabhakaran, Xiaohong Qin, Seeram Ramakrishna
To engineer bone tissue, it is crucial to design scaffolds with micro- and nano-sized architecture imitating approximate hierarchical structure of native bone, and afford desirable biological properties by introducing biocompatible polymers and bioceramics into the scaffolds. Here, a novel scaffold consisting of poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV)/polyaspartic acid (PAA) was fabricated by electrospinning and nano-hydroxyapatite (nHA) was deposited by calcium-phosphate dipping process for bone tissue regeneration...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28620190/formation-of-biphasic-hydroxylapatite-beta-magnesium-tricalcium-phosphate-in-heat-treated-salmonid-vertebrae
#11
Don H Butler, Ruth Shahack-Gross
Ichthyoarchaeological evidence is uncommon at ancient hunter-gatherer sites from various regions and timeframes. This research contributes to the development of microarchaeological techniques useful for identifying fishing economies in situations where classifiable bones are unavailable. Specifically, traces of heat altered bone mineral in domestic hearths are expected to provide markers for discarded fish remains. We used a series of laboratory incineration experiments to characterize the mineralogy of burned salmonid vertebrae...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616254/in-vitro-ion-adsorption-and-cytocompatibility-of-dicalcium-phosphate-ceramics
#12
Martha Schamel, Jake E Barralet, Jürgen Groll, Uwe Gbureck
BACKGROUND: In vitro cell testing of degradable bioceramics such as brushite or monetite is often challenging due to the ion release into or adsorption from the culture medium. These ionic changes are then mostly responsible for cell proliferation and activity, which prohibits the investigation of effects originating from surface topography or further material modifications. METHODS: Here, we aimed to solve this problem by developing a pre-conditioning regime following the repeated immersion of brushite and monetite samples in various Ca(2+), Mg(2+) and PO4(3-) containing electrolytes, followed by studying ion adsorption / release as well as changes in phase composition and in vitro cytocompatibility with MG63 cells...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/28600540/electric-field-assisted-3d-fibrous-bioceramic-based-scaffolds-for-bone-tissue-regeneration-fabrication-characterization-and-in-vitro-cellular-activities
#13
Minseong Kim, Hui-Suk Yun, Geun Hyung Kim
Nano/microfibrous structure can induce high cellular activities because of the topological similarity of the extracellular matrix, and thus, are widely used in various tissue regenerative materials. However, the fabrication of a bioceramic (high weight percent)-based 3D microfibrous structure is extremely difficult because of the low process-ability of bioceramics. In addition, three-dimensional (3D) microfibrous structure can induce more realistic cellular behavior when compared to that of 2D fibrous structure...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28600538/an-innovative-cell-laden-%C3%AE-tcp-collagen-scaffold-fabricated-using-a-two-step-printing-process-for-potential-application-in-regenerating-hard-tissues
#14
Won Jin Kim, Hui-Suk Yun, Geun Hyung Kim
Cell-laden scaffolds are widely investigated in tissue engineering because they can provide homogenous cell distribution after long culture periods, and deposit multiple types of cells into a designed region. However, producing a bioceramic 3D cell-laden scaffold is difficult because of the low processability of cell-loaded bioceramics. Therefore, designing a 3D bioceramic cell-laden scaffold is important for ceramic-based tissue regeneration. Here, we propose a new strategy to fabricate an alpha-tricalcium-phosphate (α-TCP)/collagen cell-laden scaffold, using preosteoblasts (MC3T3-E1), in which the volume fraction of the ceramic exceeded 70% and was fabricated using a two-step printing process...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28576047/optimally-designed-collagen-polycaprolactone-biocomposites-supplemented-with-controlled-release-of-ha-tcp-rhbmp-2-and-ha-tcp-prp-for-hard-tissue-regeneration
#15
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Collagen has been widely used as a very promising material to regenerate various tissues. It is a chief component of the extracellular matrix, and encourages various biological effects conducive to tissue regeneration. However, poor mechanical stability, low processability, and high level of water absorption can lead to impaired control of growth factor release and have impeded the use of collagen as a functional biomedical scaffold. Here, to overcome the shortcomings of collagen scaffolds, we have additively manufactured collagen/polycaprolactone (PCL) biocomposites supplemented with a bioceramic (hydroxyapatite (HA)/β-tricalcium-phosphate (TCP)) and two growth factors (recombinant human bone morphogenetic protein-2 [rhBMP-2] and platelet-rich plasma [PRP])...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28559709/comparative-radiographic-assessment-of-a-new-bioceramic-based-root-canal-sealer
#16
Dana Hrab, Andrea Maria Chisnoiu, Mindra Eugenia Badea, Marioara Moldovan, Radu Marcel Chisnoiu
BACKGROUND AND AIMS: The aim of this study was to assess the radiopacity of two bioceramic-based root canal sealers, the conventional TotalFill BC sealer (FKG Dentaire Switzerland) and a new experimental filling material developed in collaboration with 'Raluca Ripan' Institute for Research in Chemistry, Cluj-Napoca. METHODS: Five disc samples were prepared using both materials (10 mm diameter × 1 mm thickness), being subjected to digital radiography together with aluminum step wedges (1 to 12 mm in thickness), in accordance with ISO 6876: 2012...
2017: Clujul Medical (1957)
https://www.readbyqxmd.com/read/28544592/direct-influence-of-titanium-and-zirconia-particles-on-the-morphology-and-functionality-of-mature-human-osteoclasts
#17
Juliane Pasold, Jana Markhoff, Jenny Tillmann, Martin Krogull, Phillip Pisowocki, Rainer Bader
Within the last ten years of biomedical implants, the focus is increasingly on bioceramics, specifically on zirconia (ZrO2 ). Hence, we analyzed the impact of ZrO2 particles in comparison to titanium particles on mature human osteoclasts (OCs) as little is known about the direct effect of wear particles on mature OCs and their role in the osteolytic process during aseptic endoprosthesis loosening. Changes in cell morphology and functionality of OCs incubated with particles in different concentrations were investigated in vitro...
May 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28531999/zro2-toughened-al2o3-based-near-net-shaped-femoral-head-unique-fabrication-approach-3d-microstructure-burst-strength-and-muscle-cell-response
#18
Debasish Sarkar, Sourav Mandal, B S Reddy, Nitu Bhaskar, D C Sundaresh, Bikramjit Basu
The development of reliable biomedical devices demands the use of an integrated manufacturing protocol with comprehensive understanding of prototype characterization in terms of 3D microstructural analysis along with biocompatibility. While addressing these issues for ZrO2 (3mol% Y2O3 stabilized)-toughened Al2O3-based femoral head prototypes, the present work reports a unique fabrication protocol involving a sequence of uniaxial compaction followed by pre-sintering, machining, final sintering and polishing to ensure dimensional tolerance with respect to the design of patient-specific femoral head...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28527187/quantitative-stereological-analysis-of-the-highly-porous-hydroxyapatite-scaffolds-using-x-ray-cm-and-sem
#19
Justyna Zygmuntowicz, Aneta Zima, Joanna Czechowska, Karol Szlazak, Anna Ślosarczyk, Katarzyna Konopka
BACKGROUND: Material properties of the scaffolds as well as their microstructure are vital in determining in vivo cellular response. Three-dimensional (3D), highly porous scaffolds are used in tissue engineering to provide a suitable microenvironment and to support regeneration of bone. Both pore sizes and their architecture, in particular interconnection density, impact functionality of scaffold during its biomedical applications. OBJECTIVE: In this paper a comparative study of the microstructure of highly porous hydroxyapatite scaffolds produced via gelcasting of foamed slurries and replication of polyurethane sponge were carried out...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28512480/effect-of-intra-canal-calcium-hydroxide-remnants-on-the-push-out-bond-strength-of-two-endodontic-sealers
#20
Sholeh Ghabraei, Behnam Bolhari, Fatemeh Yaghoobnejad, Naghmeh Meraji
INTRODUCTION: The aim of this study was to evaluate the effect of intra-canal calcium hydroxide (CH) remnants after ultrasonic irrigation and hand file removal on the push out bond strength of AH-26 and EndoSequence Bioceramic sealer (BC Sealer). METHODS AND MATERIALS: A total of 102 single-rooted extracted human teeth were used in this study. After root canal preparation up to 35/0.04 Mtwo rotary file, all the specimens received CH dressing except for 34 specimens in the control group...
2017: Iranian Endodontic Journal
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