Yang Zhao, Sainan Liu, Zhili Sheng, Xue Li, Yanan Chang, Weichang Dai, Sui Kiat Chang, Junmei Liu, Yuchun Yang
To investigate the effect and mechanism of pinolenic acid (PNA) on H2 O2 -induced oxidative stress injury in HepG2 cells. Methods: PNA was used to regulate oxidative stress injury of HepG2 cells induced by H2 O2 . Quantification of cell survival rate, accumulation of intracellular reactive oxygen species (ROS), and expression levels of anti-oxidation-related genes were determined using MTT, fluorescent probe technology (DCFH-DA), and real-time quantitative reverse transcription polymerase chain technology (qRT-PCR) method, respectively...
October 2021: Food Science & Nutrition