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https://www.readbyqxmd.com/read/28544415/inertial-microfluidic-cell-stretcher-imcs-fully-automated-high-throughput-and-near-real-time-cell-mechanotyping
#1
Yanxiang Deng, Steven P Davis, Fan Yang, Kevin S Paulsen, Maneesh Kumar, Rebecca Sinnott DeVaux, Xianhui Wang, Douglas S Conklin, Assad Oberai, Jason I Herschkowitz, Aram J Chung
Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure cell mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming and labor-intensive processes, not allowing techniques to be practically used for cell biology research applications...
May 23, 2017: Small
https://www.readbyqxmd.com/read/28543392/mechanical-analysis-of-the-vascularized-fibular-graft-prosthetic-composite-vfgpc-for-internal-hemipelvectomy-reconstruction
#2
Jonathan Morris, Rui Yang, Michael Roth, Jonathan Gill, Richard Gorlick, Yungtai Lo, Bang H Hoang, Evan Garfein, David S Geller
INTRODUCTION: The vascularized fibular graft prosthetic composite (VFGPC) is used for reconstruction after internal hemipelvectomy. The purpose of this study was to create a mathematical model that calculates the mechanical effects of the vascularized fibular graft on the VFGPC. METHODS: The effects of the VFG positioning were calculated based on three-dimensional static analyzes to determine the direction, magnitude, and distribution of the forces through the prosthesis and VFG...
May 22, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28543319/glycocalyx-bending-by-an-electric-field-increases-cell-motility
#3
Francis X Hart, John R Palisano
The application of physiological strength electric fields may produce a wide range of effects on cells. The mechanisms by which cells detect the presence of these fields, however, are not fully understood. Previous experiments have shown that directionality of cells in the field is governed by an electromechanical mechanism in which the field exerts a torque on the negatively charged, inner glycocalyx that is then transmitted as a force on the cytoskeleton. This mechanism is similar to that by which cells detect fluid shear forces...
May 24, 2017: Bioelectromagnetics
https://www.readbyqxmd.com/read/28542533/ultrasonography-methods-for-predicting-malignancy-in-canine-mammary-tumors
#4
Marcus Antonio Rossi Feliciano, Ricardo Andrés Ramirez Uscategui, Marjury Cristina Maronezi, Ana Paula Rodrigues Simões, Priscila Silva, Beatriz Gasser, Leticia Pavan, Cibele Figueira Carvalho, Júlio Carlos Canola, Wilter Ricardo Russiano Vicente
The aim of this study was to evaluate and compare the efficacy of B-mode, Doppler, contrast-enhanced ultrasonography (CEUS), and Acoustic Radiation Force Impulse (ARFI) elastography in predicting malignancy in canine mammary masses. This was a prospective cohort study from 2014 to 2016, which included 153 bitches with one or more mammary masses. A total of 300 masses were evaluated by ultrasonography (B-mode, Doppler, CEUS, and ARFI) and subsequently classified as benign or malignant by histopathology. Each ultrasound parameters studied were compared between benign and malignant masses by Chi-square or Student tests and differences were considered significant when P < 0...
2017: PloS One
https://www.readbyqxmd.com/read/28541023/correlation-between-biological-characteristics-of-thrombus-and-short-term-efficiency-of-thrombolytic-therapy-by-acoustic-radiation-force-impulse-imaging-technique
#5
Xiaolei Yi, Caifang Ni, Mingming Li, Qiong Wu, Yi Li
BACKGROUND: Evaluate the biological characteristics of lower extremity deep venous thrombosis by applying acoustic radiation force impulse imaging technique(ARFI), explore the correlation between the value of shear wave speed (SWV) and short-term efficacy of thrombolytic therapy as well as analyze the application value of shear wave velocity in predicting the short-term efficacy of thrombolytic therapy. METHODS: Examine acoustic radiation force impulse imaging for 120 patients with lower extremity deep venous thrombosis and measure the shear wave velocity values...
May 24, 2017: International Angiology: a Journal of the International Union of Angiology
https://www.readbyqxmd.com/read/28540469/a-3d-microfluidic-perfusion-system-made-from-glass-for-multiparametric-analysis-of-stimulus-secretioncoupling-in-pancreatic-islets
#6
Torben Schulze, Kai Mattern, Eike Früh, Lars Hecht, Ingo Rustenbeck, Andreas Dietzel
Microfluidic perfusion systems (MPS) are well suited to perform multiparametric measurements with small amounts of tissue to function as an Organ on Chip device (OOC). Such microphysiolgical characterization is particularly valuable in research on the stimulus-secretion-coupling of pancreatic islets. Pancreatic islets are fully functional competent mini-organs, which serve as fuel sensors and transduce metabolic activity into rates of hormone secretion. To enable the simultaneous measurement of fluorescence and oxygen consumption we designed a microfluidic perfusion system from borosilicate glass by 3D femtosecond laser ablation...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28533383/mechanosensing-of-shear-by-pseudomonas-aeruginosa-leads-to-increased-levels-of-the-cyclic-di-gmp-signal-initiating-biofilm-development
#7
Christopher A Rodesney, Brian Roman, Numa Dhamani, Benjamin J Cooley, Ahmed Touhami, Vernita D Gordon
Biofilms are communities of sessile microbes that are phenotypically distinct from their genetically identical, free-swimming counterparts. Biofilms initiate when bacteria attach to a solid surface. Attachment triggers intracellular signaling to change gene expression from the planktonic to the biofilm phenotype. For Pseudomonas aeruginosa, it has long been known that intracellular levels of the signal cyclic-di-GMP increase upon surface adhesion and that this is required to begin biofilm development. However, what cue is sensed to notify bacteria that they are attached to the surface has not been known...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28526627/dynamic-fatigue-measurement-of-human-erythrocytes-using-dielectrophoresis
#8
Yuhao Qiang, Jia Liu, E Du
Erythrocytes must undergo severe deformation to pass through narrow capillaries and submicronic splenic slits for several hundred thousand times in their normal lifespan. Studies of erythrocyte biomechanics have been mainly focused on cell deformability and rheology measured from a single application of stress and mostly under a static or quasi-static state using classical biomechanical techniques, such as optical tweezers and micropipette aspiration. Dynamic behavior of erythrocytes in response to cyclic stresses that contributes to the membrane failure in blood circulation is not fully understood...
May 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28525548/computational-fluid-dynamics-characterization-of-pulsatile-flow-in-central-and-sano-shunts-connected-to-the-pulmonary-arteries-importance-of-graft-angulation-on-shear-stress-induced-platelet-mediated-thrombosis
#9
Robert Ascuitto, Nancy Ross-Ascuitto, Martin Guillot, Carey Celestin
OBJECTIVES: Central (aorta) and Sano (right ventricle)-to-pulmonary artery (PA) shunts, palliative operations for infants with complex heart defects, can develop life-threatening thrombosis. We employed computational fluid dynamics (CFD) to study pulsatile flow in these shunts, with the goal to identify haemodynamic characteristics conducive to thrombus formation. METHODS: CFD, using the finite volume method with cardiac catheterization data, and computer simulations, based on angiography, were employed to determine flow-velocity field, wall shear stress (WSS) profile and oscillatory shear index (OSI)...
May 19, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28518083/bronchoalveolar-lavage-of-murine-lungs-to-analyze-inflammatory-cell-infiltration
#10
Lien Van Hoecke, Emma Richelle Job, Xavier Saelens, Kenny Roose
Bronchoalveolar Lavage (BAL) is an experimental procedure that is used to examine the cellular and acellular content of the lung lumen ex vivo to gain insight into an ongoing disease state. Here, a simple and efficient method is described to perform BAL on murine lungs without the need of special tools or equipment. BAL fluid is isolated by inserting a catheter in the trachea of terminally anesthetized mice, through which a saline solution is instilled into the bronchioles. The instilled fluid is gently retracted to maximize BAL fluid retrieval and to minimize shearing forces...
May 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516388/effects-of-different-selenium-sources-on-growth-performance-antioxidant-capacity-and-meat-quality-of-local-chinese-subei-chickens
#11
J L Li, L Zhang, Z Y Yang, Z Y Zhang, Y Jiang, F Gao, G H Zhou
Despite increasing evidence indicating the essential involvement of selenium (Se) on growth performance, antioxidant capacity, and meat quality of commercial broilers, the effects of different Se sources on local Chinese Subei chickens is unclear. A total of 360 50-day-old male chickens were individually weighed and randomly allocated to four treatment groups. Chickens in each of the four groups were fed diets supplemented with 0.3 mg Se/kg as sodium Se (SS), Se-enriched yeast (SY), selenomethionine (Met-Se), or nano red element Se (Nano-Se) for 40 days...
May 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28515439/the-biomechanics-behind-extreme-osteophagy-in-tyrannosaurus-rex
#12
Paul M Gignac, Gregory M Erickson
Most carnivorous mammals can pulverize skeletal elements by generating tooth pressures between occluding teeth that exceed cortical bone shear strength, thereby permitting access to marrow and phosphatic salts. Conversely, carnivorous reptiles have non-occluding dentitions that engender negligible bone damage during feeding. As a result, most reptilian predators can only consume bones in their entirety. Nevertheless, North American tyrannosaurids, including the giant (13 metres [m]) theropod dinosaur Tyrannosaurus rex stand out for habitually biting deeply into bones, pulverizing and digesting them...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28515328/mechanotransmission-in-endothelial-cells-subjected-to-oscillatory-and-multi-directional-shear-flow
#13
Mahsa Dabagh, Payman Jalali, Peter J Butler, Amanda Randles, John M Tarbell
Local haemodynamics are linked to the non-uniform distribution of atherosclerosic lesions in arteries. Low and oscillatory (reversing in the axial flow direction) wall shear stress (WSS) induce inflammatory responses in endothelial cells (ECs) mediating disease localization. The objective of this study is to investigate computationally how the flow direction (reflected in WSS variation on the EC surface over time) influences the forces experienced by structural components of ECs that are believed to play important roles in mechanotransduction...
May 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28514259/does-type-1-diabetes-mellitus-affect-the-shear-wave-velocity-of-the-thyroid-gland-of-children-without-autoimmune-thyroiditis
#14
Dilek Sağlam, Meltem Ceyhan Bilgici, Cengiz Kara, Gülay Can Yilmaz, Asli Tanrivermiş Sayit
OBJECTIVE: The aim of this study is to evaluate the shear wave velocity (SWV) of the thyroid gland with acoustic radiation force impulse elastography in children with type 1 diabetes mellitus (T1D). MATERIALS AND METHODS: Between November 2015 and April 2016, 35 T1D patients who were referred to our hospital's endocrinology outpatient clinic (mean age, 11.88 ± 4.1 years) and 30 children (mean age, 11.3 ± 3.08 years) in the control group were enrolled in the study...
May 16, 2017: Ultrasound Quarterly
https://www.readbyqxmd.com/read/28513698/unzipping-and-shearing-dna-with-electrophoresed-nanoparticles-in-hydrogels
#15
Keitel Cervantes-Salguero, Ibuki Kawamata, Shin-Ichiro M Nomura, Satoshi Murata
We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm(-1)) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.
May 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28513280/application-of-acoustic-radiation-force-impulse-arfi-elastography-in-imaging-of-delayed-onset-muscle-soreness-doms-a-comparative-analysis-with-3t-mri
#16
Thilo Hotfiel, Marion Kellermann, Bernd Swoboda, Dane Wildner, Tobias Golditz, Casper Grim, Martin Raithel, Michael Uder, Rafael Heiss
CONTEXT: DOMS is one of the most common reasons for impaired muscle performance in sports and is associated with reduced muscle strength and frequently observed both in professional and recreational athletes. OBJECTIVE: To emphasize the diagnostic value of Acoustic Radiation Force Impulse (ARFI) in imaging of Delayed Onset Muscle Soreness by comparing findings to high-resolution 3T MRI T2 weighted sequences. DESIGN: Case series. SETTING: Laboratory environment...
May 17, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28507125/effective-control-of-complex-turbulent-dynamical-systems-through-statistical-functionals
#17
Andrew J Majda, Di Qi
Turbulent dynamical systems characterized by both a high-dimensional phase space and a large number of instabilities are ubiquitous among complex systems in science and engineering, including climate, material, and neural science. Control of these complex systems is a grand challenge, for example, in mitigating the effects of climate change or safe design of technology with fully developed shear turbulence. Control of flows in the transition to turbulence, where there is a small dimension of instabilities about a basic mean state, is an important and successful discipline...
May 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28505719/active-rotational-and-translational-microrheology-beyond-the-linear-spring-regime
#18
Lachlan J Gibson, Shu Zhang, Alexander B Stilgoe, Timo A Nieminen, Halina Rubinsztein-Dunlop
Active particle tracking microrheometers have the potential to perform accurate broadband measurements of viscoelasticity within microscopic systems. Generally, their largest possible precision is limited by Brownian motion and low frequency changes to the system. The signal to noise ratio is usually improved by increasing the size of the driven motion compared to the Brownian as well as averaging over repeated measurements. New theory is presented here whereby error in measurements of the complex shear modulus can be significantly reduced by analyzing the motion of a spherical particle driven by nonlinear forces...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28504166/functionality-of-chitosan-in-batter-formulations-for-coating-of-fish-sticks-effect-on-physicochemical-quality
#19
K A Martin Xavier, Hauzoukim, Nagalakshmi Kannuchamy, Amjad K Balange, M K Chouksey, Venkateshwarlu Gudipati
Chitosan is a natural polymer having diverse applications in food industry. The present study was undertaken to evaluate chitosan as a hydrocolloid in batter composition in developing enrobed fish sticks with better functional properties and improved quality. Different concentrations of chitosan gel were made in acetic acid medium and incorporated into batter for making enrobed fish sticks. Coating parameters, fat uptake, oil reduction, chemical quality parameters, instrumental texture analysis and colour were studied...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28501711/protection-of-cortex-by-overlying-meninges-tissue-during-dynamic-indentation-of-the-adolescent-brain
#20
David B MacManus, Baptiste Pierrat, Jeremiah G Murphy, Michael D Gilchrist
Traumatic brain injury (TBI) has become a recent focus of biomedical research with a growing international effort targeting material characterization of brain tissue and simulations of trauma using computer models of the head and brain to try to elucidate the mechanisms and pathogenesis of TBI. The meninges, a collagenous protective tri-layer which encloses the entire brain and spinal cord has been largely overlooked in these material characterization studies. This has resulted in a lack of accurate constitutive data for the cranial meninges, particularly under dynamic conditions such as those experienced during head impacts...
May 10, 2017: Acta Biomaterialia
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