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JOURNAL ARTICLE
Caitríona Grönberg, Sara Rattik, Cuong Tran-Manh, Xiang Zhou, Aleix Rius Rigau, Yi-Nan Li, Andrea-Hermina Györfi, Nicholas Dickel, Meik Kunz, Alexander Kreuter, Emil-Alexandru Matei, Honglin Zhu, Petter Skoog, David Liberg, Jörg Hw Distler, Thuong Trinh-Minh
BACKGROUND: The interleukin (IL)-1 receptor accessory protein (IL1RAP) is an essential coreceptor required for signalling through the IL-1, IL-33 and IL-36 receptors. Here, we investigate the antifibrotic potential of the combined inhibition of these cytokines by an anti-IL1RAP antibody to provide a scientific background for clinical development in systemic sclerosis (SSc). METHODS: The expression of IL1RAP-associated signalling molecules was determined by data mining of publicly available RNA sequencing (RNAseq) data as well as by imaging mass cytometry...
April 9, 2024: Annals of the Rheumatic Diseases
#22
JOURNAL ARTICLE
Ching-Yi Lee, Yu-Cheng Chen, Yen-Hua Huang, Yi Lien, Cheng-Yang Huang
The carnivorous pitcher plants of the genus Nepenthes have long been known for their ethnobotanical applications. In this study, we prepared various extracts from the pitcher, stem, and leaf of Nepenthes miranda using 100% ethanol and assessed their inhibitory effects on key enzymes related to skin aging, including elastase, tyrosinase, and hyaluronidase. The cytotoxicity of the stem extract of N. miranda on H838 human lung carcinoma cells were also characterized by effects on cell survival, migration, proliferation, apoptosis induction, and DNA damage...
March 11, 2024: Plants (Basel, Switzerland)
#23
JOURNAL ARTICLE
Xudong Sang, Tian Gan, Gai Ge, Dan Li, Youming Mei, Chun Pan, Siyu Long, Bibo Xie, Xiaobing Yu, Zhiming Chen, Hongsheng Wang
BACKGROUND: Psoriasis, a systemic disorder mediated by the immune system, can appear on the skin, joints, or both. Individuals with cutaneous psoriasis (PsC) have an elevated risk of developing psoriatic arthritis (PsA) during their lifetime. Despite this known association, the cellular and molecular mechanisms underlying this progression remain unclear. METHODS: We performed high-dimensional, in-depth immunophenotyping of peripheral blood mononuclear cells (PBMCs) in patients with PsA and psoriasis vulgaris (PsV) by mass cytometry...
2024: Journal of Immunology Research
#24
Debduti Mukhopadhyay, Taher Sbitli, Anandita Kishore, Elijah R Ilasin, Umair Masood
This case report presents a rare and intriguing clinical scenario involving a 63-year-old male with recurrent left-sided hydroureteronephrosis, hypertension, and hyperlipidemia presenting with fatigue, dyspnea, and weight loss. Laboratory findings revealed anemia, basophilic stippling, spherocytosis, and nucleated red blood cells on the peripheral blood smear, raising concerns for hemolysis. Concomitant iron deficiency anemia led to further investigations, revealing gastritis and a colonic mass. A CT scan revealed splenomegaly with an accessory spleen...
March 2024: Curēus
#25
JOURNAL ARTICLE
Fang Lyu, Sihuai Huang, Zhecheng Yan, Qingliu He, Chunyu Liu, Lulin Cheng, Yukun Cong, Kang Chen, Yarong Song, Yifei Xing
The development of resistance to cisplatin (CDDP) in bladder cancer presents a notable obstacle, with indications pointing to the substantial role of circular RNAs (circRNAs) in this resistance. Nevertheless, the precise mechanisms through which circRNAs govern resistance are not yet fully understood. Our findings demonstrate that circUGGT2 is significantly upregulated in bladder cancer, facilitating cancer cell migration and invasion. Additionally, our analysis of eighty patient outcomes revealed a negative correlation between circUGGT2 expression levels and prognosis...
April 5, 2024: Cellular Signalling
#26
JOURNAL ARTICLE
Katey S S Enfield, Emma Colliver, Claudia S Y Lee, Alastair Magness, David A Moore, Monica Sivakumar, Kristiana Grigoriadis, Oriol Pich, Takahiro Karasaki, Philip S Hobson, Dina Levi, Selvaraju Veeriah, Clare Puttick, Emma L Nye, Mary Green, Krijn K Dijkstra, Masako Shimato, Ayse U Akarca, Teresa Marafioti, Roberto Salgado, Allan Hackshaw, TRACERx Consortium, Mariam Jamal-Hanjani, Febe van Maldegem, Nicholas McGranahan, Benjamin Glass, Hanna Pulaski, Eric Walk, James L Reading, Sergio A Quezada, Crispin T Hiley, Julian Downward, Erik Sahai, Charles Swanton, Mihaela Angelova
Understanding the role of the tumour microenvironment (TME) in lung cancer is critical to improving patient outcome. We identified four histology-independent archetype TMEs in treatment-naive early-stage lung cancer using imaging mass cytometry in the TRACERx study (n=81 patients/198 samples/2.3million cells). In immune-hot adenocarcinomas, spatial niches of T cells and macrophages increased with clonal neoantigen burden, whereas such an increase was observed for niches of plasma and B cells in immune-excluded squamous cell carcinomas (LUSC)...
April 6, 2024: Cancer Discovery
#27
JOURNAL ARTICLE
Zijan Wang, Pedro C Ciarlini, Kwadwo A Oduro, Ramya Gadde, Stacey O'Neill, Chen Zhao, Howard J Meyerson
OBJECTIVES: We assessed the utility of red blood cell (RBC) CD105 and side scatter (SSC) parameters by flow cytometry for the detection of low-grade myelodysplastic neoplasms (MDS) in bone marrow specimens. METHODS: Ten RBC parameters incorporating CD105 or SSC combined with the Meyerson-Alayed scoring system (MASS) metrics were retrospectively evaluated by flow cytometry for utility in detecting low-grade MDS (n = 56) compared with cytopenic controls (n = 86)...
April 6, 2024: American Journal of Clinical Pathology
#28
JOURNAL ARTICLE
Gan Gao, Xiang-Yang Bao, Qian-Nan Wang, Xiao-Peng Wang, Fang-Bin Hao, Si-Meng Liu, Min-Jie Wang, Qing-Bao Guo, Jing-Jie Li, Lian Duan
OBJECTIVE: This study aimed to investigate whether high homocysteine (Hcy) levels associated with the MTHFR gene influence the formation of the collateral vascular network in patients with moyamoya disease (MMD) after encephaloduroarteriosynangiosis (EDAS) by influencing the number of endothelial progenitor cells (EPCs) in peripheral blood. METHODS: A total of 118 Chinese patients with bilateral primary MMD were prospectively included. Blood samples were collected from the anterior cubital vein before surgery, and MTHFR rs9651118 was genotyped using high-throughput mass spectrometry to determine the genotype of the test specimen...
April 5, 2024: Journal of Neurosurgery
#29
JOURNAL ARTICLE
Ricardo Calderon-Gonzalez, Amy Dumigan, Joana Sá-Pessoa, Adrien Kissenpfennig, José A Bengoechea
In vivo single-cell approaches have transformed our understanding of the immune populations in tissues. Mass cytometry (CyTOF), that combines the resolution of mass spectrometry with the ability to conduct multiplexed measurements of cell molecules at the single cell resolution, has enabled to resolve the diversity of immune cell subsets, and their heterogeneous functionality. Here we assess the feasibility of taking CyTOF one step further to immuno profile cells while tracking their interactions with bacteria, a method we term Bac-CyTOF...
April 5, 2024: PLoS Pathogens
#30
JOURNAL ARTICLE
Anupama Bahadur, Rajlaxmi Mundhra, Poonam Gill, Ashok Singh
Involvement of the cervix with acute lymphoblastic leukaemia (ALL) is extremely rare. In this case report, we discuss an unmarried woman in her early 20s, who presented in the emergency with lower abdominal pain and irregular vaginal bleeding for 1 month. Clinical examination and imaging revealed a large cervical mass probably neoplastic with obstructive uropathy. On evaluation, she was diagnosed incidentally with CALLA-positive precursor B cell ALL in peripheral blood flow cytometry. Involvement of B cell ALL in cervical mass was confirmed by histopathological examination of cervical biopsy and immunohistochemistry markers...
April 2, 2024: BMJ Case Reports
#31
JOURNAL ARTICLE
Solange Torres, Fabián Figueroa, Virginia Casas Arrojo, Cristian Riquelme, Allisson Astuya, Roberto T Abdala-Díaz, José Becerra, Mario Rajchenberg, María Belén Pildain, Juliano Marcon Baltazar, Jaime R Cabrera-Pardo, Claudia Pérez
Polysaccharides from wood-rooting fungi have attracted attention due to their broad pharmacological properties. Herein, we report the antitumor and immunomodulatory activities of acid polysaccharides isolated from fungi Gloeosoma mirabile. The polysaccharide extracts displayed significant antiproliferative activity against cancer cell lines (MCF-7, HCT-116, U-937) in a dose-dependent manner and induction of IL-6 in macrophage RAW 264.7. Furthermore, flow cytometry analysis showed that high polysaccharide concentrations induced apoptosis by 83% in HL-60 cells...
April 3, 2024: Natural Product Research
#32
JOURNAL ARTICLE
Jasmine Gujral, Brian A Kidd, Christine Becker, Eddye Golden, Hao-Chih Lee, Seunghee Kim-Schulze, Mabel Yau, Joel Dudley, Robert Rapaport
INTRODUCTION: Growth hormone (GH) and the immune system have multiple bidirectional interactions. Data about the acute effects of GH on the immune system are lacking. The objective of our study was to evaluate the acute effects of GH on the immune system using time-of-flight mass cytometry. METHODS: This was a prospective study of pediatric patients who were being evaluated for short stature and underwent a GH stimulation test at a tertiary care center. Blood samples for immunologic markers, i...
April 2024: Curēus
#33
JOURNAL ARTICLE
Peicong Ge, Chuming Tao, Wenjing Wang, Qiheng He, Chenglong Liu, Zhiyao Zheng, Siqi Mou, Bojian Zhang, Xingju Liu, Qian Zhang, Rong Wang, Hao Li, Dong Zhang, Jizong Zhao
BACKGROUND: Moyamoya disease (MMD) stands as a prominent cause of stroke among children and adolescents in East Asian populations. Although a growing body of evidence suggests that dysregulated inflammation and autoimmune responses might contribute to the development of MMD, a comprehensive and detailed understanding of the alterations in circulating immune cells associated with MMD remains elusive. METHODS: In this study, we employed a combination of single-cell RNA sequencing (scRNA-seq), mass cytometry and RNA-sequencing techniques to compare immune cell profiles in peripheral blood samples obtained from patients with MMD and age-matched healthy controls...
April 2024: Clinical and Translational Medicine
#34
JOURNAL ARTICLE
Jing Wang, Zhong-Jin Chen, Ze-Yi Zhang, Mei-Ping Shen, Bo Zhao, Wei Zhang, Ye Zhang, Ji-Gang Lei, Cheng-Jie Ren, Jing Chang, Cui-Li Xu, Meng Li, Yang-Yang Pi, Tian-Lun Lu, Cheng-Xiang Dai, Su-Ke Li, Ping Li
BACKGROUND: Human adipose stromal cells-derived extracellular vesicles (haMSC-EVs) have been shown to alleviate inflammation in acute lung injury (ALI) animal models. However, there are few systemic studies on clinical-grade haMSC-EVs. Our study aimed to investigate the manufacturing, quality control (QC) and preclinical safety of clinical-grade haMSC-EVs. METHODS: haMSC-EVs were isolated from the conditioned medium of human adipose MSCs incubated in 2D containers...
April 2, 2024: Stem Cell Research & Therapy
#35
JOURNAL ARTICLE
Laise Rodrigues Reis, Ismael Artur Costa-Rocha, Thais Abdala-Torres, Ana Carolina Campi-Azevedo, Vanessa Peruhype-Magalhães, Márcio Sobreira Silva Araújo, Elaine Spezialli, Lis Ribeiro do Valle Antonelli, Rosiane Aparecida da Silva-Pereira, Gregório Guilherme Almeida, Eder Gatti Fernandes, Francieli Fontana Sutile Tardetti Fantinato, Carla Magda Allan Santos Domingues, Maria Cristina Ferreira Lemos, Alexandre Chieppe, Jandira Aparecida Campos Lemos, Jordana Grazziela Coelho-Dos-Reis, Sheila Maria Barbosa de Lima, Adriana de Souza Azevedo, Waleska Dias Schwarcz, Luiz Antônio Bastos Camacho, Maria de Lourdes de Sousa Maia, Tatiana Guimarães de Noronha, Caroline Duault, Yael Rosenberg-Hasson, Andréa Teixeira-Carvalho, Holden Terry Maecker, Olindo Assis Martins-Filho
The present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features of cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF vaccine, administered as a single subcutaneous injection. The immunological parameters of each volunteer was monitored at two time points, referred as: before (Day 0) [Non-Vaccinated, NV(D0) ] and after vaccination (Day 30-45) [Primary Vaccinees, PV(D30-45) ]. Data demonstrated high levels of neutralizing antibodies for PV(D30-45) leading to a seropositivity rate of 93%...
April 2, 2024: Scientific Reports
#36
Qian Zhu, Akhila Balasubramanian, Jaya Ruth Asirvatham, Danthasinghe Waduge Badrajee Piyarathna, Jaspreet Kaur, Nada Mohamed, Ling Wu, Megha Chatterjee, Stacy Wang, Niloufar Pourfarrokh, Uttam Rasaily, Yitian Xu, Junjun Zheng, Deborah Jebakumar, Arundathi Rao, Shu-Hsia Chen, Yi Li, Eric Chang, Xiaoxian Li, Ritu Aneja, Xiang H-F Zhang, Arun Sreekumar
Racial disparities in triple-negative breast cancer (TNBC) outcomes have been reported. However, the biological mechanisms underlying these disparities remain unclear. We integrated imaging mass cytometry and spatial transcriptomics, to characterize the tumor microenvironment (TME) of African American (AA) and European American (EA) patients with TNBC. The TME in AA patients was characterized by interactions between endothelial cells, macrophages, and mesenchymal-like cells, which were associated with poor patient survival...
March 31, 2024: bioRxiv
#37
JOURNAL ARTICLE
Xuemei Xu, Zhaojingtao Zhu, Siyu Chen, Yanneng Fu, Jinxia Zhang, Yangyang Guo, Zhouyang Xu, Yingying Xi, Xuebao Wang, Faqing Ye, Huijun Chen, Xiaojiao Yang
Introduction: Cancer, a significant global health concern, necessitates innovative treatments. The pivotal role of chronic inflammation in cancer development underscores the urgency for novel therapeutic strategies. Benzothiazole derivatives exhibit promise due to their distinctive structures and broad spectrum of biological effects. This study aims to explore new anti-tumor small molecule drugs that simultaneously anti-inflammatory and anticancer based on the advantages of benzothiazole frameworks. Methods: The compounds were characterized by nuclear magnetic resonance (NMR), liquid chromatograph-mass spectrometer (LC-MS) and high performance liquid chromatography (HPLC) for structure as well as purity and other related physicochemical properties...
2024: Frontiers in Chemistry
#38
JOURNAL ARTICLE
David Kaplan, Hillard M Lazarus, Eric Christian
BACKGROUND: Cytometric analysis has been commonly used to delineate distinct cell subpopulations among peripheral blood mononuclear cells by the differential expression of surface receptors. This capability has reached its apogee with high-dimensional approaches such as mass cytometry and spectral cytometry that include simultaneous assessment of 20-50 analytes. Unfortunately, this approach also engenders significant complexity with analytical and interpretational pitfalls. METHODS: Here, we demonstrate a complementary approach with restricted-dimensionality to assess cell-type specific intracellular molecular expression levels at exceptional levels of precision...
April 1, 2024: European Journal of Clinical Investigation
#39
JOURNAL ARTICLE
Sara De Biasi, Domenico Lo Tartaro, Anita Neroni, Moritz Rau, Nikolaos Paschalidis, Rebecca Borella, Elena Santacroce, Annamaria Paolini, Lara Gibellini, Alin Liviu Ciobanu, Michela Cuccorese, Tommaso Trenti, Ignacio Rubio, Francesca Vitetta, Martina Cardi, Rafael José Argüello, Diana Ferraro, Andrea Cossarizza
Disease-modifying therapies (DMT) administered to patients with multiple sclerosis (MS) can influence immune responses to SARS-CoV-2 and vaccine efficacy. However, data on the detailed phenotypic, functional and metabolic characteristics of antigen (Ag)-specific cells following the third dose of mRNA vaccine remain scarce. Here, using flow cytometry and 45-parameter mass cytometry, we broadly investigate the phenotype, function and the single-cell metabolic profile of SARS-CoV-2-specific T and B cells up to 8 months after the third dose of mRNA vaccine in a cohort of 94 patients with MS treated with different DMT, including cladribine, dimethyl fumarate, fingolimod, interferon, natalizumab, teriflunomide, rituximab or ocrelizumab...
March 29, 2024: Nature Communications
#40
JOURNAL ARTICLE
Yongjuan Chen, Aaminah Khan, Christopher Katsinas, Filip Michniewicz, Jessie Goldberg, Laura Franshaw, Maria Tsoli, David S Ziegler
BACKGROUND: Diffuse intrinsic pontine gliomas (DIPGs) pose a significant challenge as a highly aggressive and currently incurable form of pediatric brain cancer, necessitating the development of novel therapeutic strategies. Omacetaxine, an FDA-approved protein synthesis inhibitor for treating certain hematological malignancies, was investigated for its potential antitumor effects against preclinical DIPG models. METHODS: We employed primary DIPG cultures to study omacetaxine's cytotoxicity and its impact on colony formation...
2024: Neuro-oncology advances
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