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https://www.readbyqxmd.com/read/29036735/facile-synthesis-and-characterization-of-l-aspartic-acid-coated-iron-oxide-magnetic-nanoparticles-ionps-for-biomedical-applications
#1
Marziyeh Salehiabar, Hamed Nosrati, Soodabeh Davaran, Hossein Danafar, Hamidreza Kheiri Manjili
Natural L-aspartic acid coated iron oxide magnetic nanoparticles (Asp@IONPs) were prepared by a one pot, in-situ and green co-precipitation method in an aqueous medium. Functionalized iron oxide magnetic nanoparticles (IONPs) were characterized by Vibrating Sample Magnetometer (VSM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of IONPs was also investigated on HEK-293 cell lines...
October 16, 2017: Drug Research
https://www.readbyqxmd.com/read/29032783/targeted-metabolomics-new-insights-into-pathobiology-of-retained-placenta-in-dairy-cows-and-potential-risk-biomarkers
#2
E Dervishi, G Zhang, R Mandal, D S Wishart, B N Ametaj
A targeted quantitative metabolomics approach was used to study temporal changes of serum metabolites in cows that normally released their fetal membranes and those that retained the placenta. We identified and measured serum concentrations of 128 metabolites including amino acids, acylcarnitines, biogenic amines, glycerophospholipids, sphingolipids and hexose at -8 and -4 weeks before parturition, during the week of retained placenta (RP) diagnosis, and at +4 and +8 weeks after parturition. In addition, we aimed at identifying metabolite signatures of pre-RP in the serum that might be used as predictive biomarkers for risk of developing RP in dairy cows...
October 16, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29032653/bacterial-hormone-sensitive-lipases-bhsls-emerging-enzymes-for-biotechnological-applications
#3
REVIEW
T Doohun Kim
Lipases are important enzymes with biotechnological applications in dairy, detergent, food, fine chemicals, and pharmaceutical industries. Specifically, hormone-sensitive lipase (HSL) is an intracellular lipase that can be stimulated by several hormones such as catecholamine, glucagon, and adrenocorticotropic hormone (ACTH). Bacterial hormone-sensitive lipases (bHSLs), which are homologous to the C-terminal domain of HSL, have α/β-hydrolase fold with a catalytic triad composed of His, Asp, and Ser. These bHSLs could be used for a wide variety of industrial applications because of their high activity, broad substrate specificity, and remarkable stability...
October 14, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29031804/transcriptional-response-after-exposure-to-domoic-acid-producing-pseudo-nitzschia-in-the-digestive-gland-of-the-mussel-mytilus-galloprovincialis
#4
Antonio J Pazos, Pablo Ventoso, Roi Martínez-Escauriaza, M Luz Pérez-Parallé, Juan Blanco, Juan C Triviño, José L Sánchez
Bivalve molluscs are filter feeding species that can accumulate biotoxins in their body tissues during harmful algal blooms. Amnesic Shellfish Poisoning (ASP) is caused by species of the diatom genus Pseudo-nitzschia, which produces the toxin domoic acid. The Mytilus galloprovincialis digestive gland transcriptome was de novo assembled based on the sequencing of 12 cDNA libraries, six obtained from control mussels and six from mussels naturally exposed to domoic acid-producing diatom Pseudo-nitzschia australis...
October 11, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29031431/implementing-an-antibiotic-stewardship-program-at-a-long-term-acute-care-hospital-in-detroit-michigan
#5
Ammara Mushtaq, Reda A Awali, Suganya Chandramohan, Amar Krishna, Caitlin Biedron, Olufemi Jegede, Teena Chopra
BACKGROUND: The objective of the study was to assess health care providers' (HCPs) knowledge and attitude toward antimicrobial resistance (AMR) and implement an antimicrobial stewardship program (ASP) in a long-term acute care hospital (LTACH). METHODS: A questionnaire on antibiotic use and resistance was administered to HCP in an LTACH in Detroit, Michigan, between August 2011 and October 2011. Concurrently, a retrospective review of common antibiotic prescription practices and costs was conducted...
October 11, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29030429/activating-mutations-in-quorum-sensing-regulator-rgg2-and-its-conformational-flexibility-in-the-absence-of-an-intermolecular-disulfide-bond
#6
Reid V Wilkening, Glenn C Capodagli, Atul Khataokar, Kaitlyn M Tylor, Matthew B Neiditch, Michael J Federle
Rap/Rgg/NprR/PlcR/PrgX (RRNPP) quorum-sensing systems use extracellular peptide pheromones that are detected by cytoplasmic receptors to regulate gene expression in firmicute bacteria. Rgg-type receptors are allosterically regulated through direct pheromone binding to control transcriptional activity; however, the receptor activation mechanism remains poorly understood. Previous work has identified a disulfide bond between Cys-45 residues within the homodimer interface of Rgg2 from Streptococcus dysgalactiae (Rgg2Sd)...
October 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29030107/ammodytin-l-is-the-main-cardiotoxic-component-of-the-vipera-ammodytes-ammodytes-venom
#7
Svjetlana Karabuva, Boris Lukšić, Ivica Brizić, Zorica Latinović, Adrijana Leonardi, Igor Križaj
Venom of the nose-horned viper (V. a. ammodytes) as also venoms of some related European viperids can induce also cardiotoxic effects in mammals. In this work we demonstrated that the protein in the V. a. ammodytes venom acting on heart is a myotoxic secreted phospholipase A2 analogue ammodytin L (AtnL). In the isolated perfused rat heart AtnL induced significant and irreversible cardiotoxicity characterized by atrioventricular (AV) blockade. This venom protein induced appearance of high levels of creatine kinase, lactate dehydrogenase, aspartate aminotransferase and troponin I in the sinus effluent of the isolated heart, indicative for myocardial damage, which is obviously the primary cause of its cardiotoxic action...
October 10, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29028546/enzymatic-characterization-and-crystal-structure-analysis-of-chlamydomonas-reinhardtii-dehydroascorbate-reductase-and-their-implications-for-oxidative-stress
#8
Hsin-Yang Chang, Shu-Tseng Lin, Tzu-Ping Ko, Shu-Mei Wu, Tsen-Hung Lin, Yu-Ching Chang, Kai-Fa Huang, Tse-Min Lee
Dehydroascorbate reductase (DHAR) is a key enzyme for glutathione (GSH)-dependent reduction of dehydroascorbate (DHA) to recycled ascorbate (AsA) in plants, and plays a major role against the toxicity of reactive oxygen species (ROS). Previously, we proposed that the increase of AsA regeneration via enhanced DHAR activity modulates the ascorbate-glutathione cycle activity against photooxidative stress in Chlamydomonas reinhardtii. In the present work, we use site-directed mutagenesis and crystal structure analysis to elucidate the molecular basis of how C...
October 3, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29027162/how-to-combine-chip-with-qpcr
#9
Patrik Asp
Chromatin immunoprecipitation (ChIP) coupled with quantitative PCR (qPCR) has in the last 15 years become a basic mainstream tool in genomic research. Numerous commercially available ChIP kits, qPCR kits, and real-time PCR systems allow for quick and easy analysis of virtually anything chromatin-related as long as there is an available antibody. However, the highly accurate quantitative dimension added by using qPCR to analyze ChIP samples significantly raises the bar in terms of experimental accuracy, appropriate controls, data analysis, and data presentation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29026283/outcomes-study-between-femtosecond-laser-assisted-cataract-surgery-and-conventional-phacoemulsification-surgery-using-an-active-fluidics-system
#10
Wilson Takashi Hida, Patrick Frenzel Tzelikis, César Vilar, Mario Augusto Pereira Dias Chaves, Antônio Francisco Pimenta Motta, Pedro Carlos Carricondo, Bruna Vieira Ventura, Renato Ambrosio, Walton Nosé, Milton Ruiz Alves
PURPOSE: The purpose of this study was to compare intraoperative parameters between femtosecond laser-assisted cataract surgery (FLACS) and conventional phacoemulsification using Centurion(®) Vision System with Active Fluidics. SETTING: This study was performed at Brasília Ophthalmologic Hospital, Brasília, Federal District, Brazil. PATIENTS AND METHODS: This was a prospective randomized comparative study. Patients with the diagnosis of cataract and surgical extraction programmed were divided into two groups: conventional phacoemulsification and FLACS...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29025237/the-role-of-injectables-in-aesthetic-surgery-financial-implications
#11
Bryson G Richards, William F Schleicher, Gehaan F D'Souza, Raymond Isakov, James E Zins
Background: The plastic surgeon competes with both core and noncore physicians and surgeons for traditional cosmetic procedures. In 2007, the American Society for Aesthetic Plastic Surgery (ASAPS) and the American Society of Plastic Surgeons (ASPS) joined efforts to form a Cosmetic Medicine Task Force to further analyze this trend. Objectives: Our objective is to document and quantify the patient capture and total collections generated in a single surgeon's practice exclusive from Botulinum Toxin A and filler injections over a 10-year period...
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29023605/involvement-of-the-arg566-residue-of-aeromonas-sobria-serine-protease-in-substrate-specificity
#12
Hidetomo Kobayashi, Tadamune Otsubo, Fumiteru Teraoka, Kiyoshi Ikeda, Soshi Seike, Eizo Takahashi, Keinosuke Okamoto, Toru Yoshida, Hideaki Tsuge, Hiroyasu Yamanaka
Aeromonas sobria serine protease (ASP) is an extracellular serine protease secreted by the organism. Here, we identified the amino acid residue of ASP that contributes to substrate specificity by using both synthetic peptides and biological protein components. The results showed that the arginine residue at position 566 (Arg-566) of ASP, which is located in the extra occluding region of ASP close to an entrance of the catalytic cavity, is involved in the substrate specificity. A substitutional point mutation of the Arg-566 residue of ASP to Ala residue (ASP[R566A]) caused a decrease of the proteolytic efficiency for a certain substrate...
2017: PloS One
https://www.readbyqxmd.com/read/29023459/chlorophyte-aspartyl-aminopeptidases-ancient-origins-expanded-families-new-locations-and-secondary-functions
#13
Sang-Youl Park, Melissa A Scranton, Jason E Stajich, Ashley Yee, Linda L Walling
M18 aspartyl aminopeptidases (DAPs) are well characterized in microbes and animals with likely functions in peptide processing and vesicle trafficking. In contrast, there is a dearth of knowledge on plant aminopeptidases with a preference for proteins and peptides with N-terminal acidic residues. During evolution of the Plantae, there was an expansion and diversification of the M18 DAPs. After divergence of the ancestral green algae from red and glaucophyte algae, a duplication yielded the DAP1 and DAP2 lineages...
2017: PloS One
https://www.readbyqxmd.com/read/29022979/the-antibacterial-and-antibiofilm-efficacies-of-a-liposomal-peptide-originating-from-rice-bran-protein-against-listeria-monocytogenes
#14
Chuanfen Pu, Wenting Tang
With the aim of exploring a natural antilisterial peptide from food-derived origin, an antibacterial peptide named as Alpep7 was purified from the bromelain hydrolysate of rice bran protein (RBP) in this study. The resulting amino acid consequence was identified as KVDHFPL (Lys-Val-Asp-His-Phe-Pro-Leu) by ultraperformance liquid chromatography tandem matrix-assisted laser desorption/ionisation quadrupole time-of-flight mass spectrometry (MALDI Q-TOF MS). In addition, to assess the probability of the targeted delivery of liposome encapsulation of the peptide to Listeria biofilm, Alpep7-loaded liposomes were further prepared from a mixture of dipalmitoylphosphatidylcholine, stearylamine and cholesterol in a molar ratio of 10 : 3 : 2 and characterised by the analysis of particle size, zeta potential, microtopography and storage stability...
October 12, 2017: Food & Function
https://www.readbyqxmd.com/read/29021611/spatial-detection-of-fetal-marker-genes-expressed-at-low-level-in-adult-human-heart-tissue
#15
Michaela Asp, Fredrik Salmén, Patrik L Ståhl, Sanja Vickovic, Ulrika Felldin, Marie Löfling, José Fernandez Navarro, Jonas Maaskola, Maria J Eriksson, Bengt Persson, Matthias Corbascio, Hans Persson, Cecilia Linde, Joakim Lundeberg
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In many studies on heart failure, cardiac biopsies have been analyzed as uniform pieces of tissue with bulk techniques, but this homogenization approach can mask medically relevant phenotypes occurring only in isolated parts of the tissue. This study examines such spatial variations within and between regions of cardiac biopsies...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29020181/a-healthcare-improvement-intervention-combining-nucleic-acid-microarray-testing-with-direct-physician-response-for-management-of-staphyloccocus-aureus-bacteremia
#16
Joshua C Eby, Morgan M Richey, James A Platts-Mills, Amy J Mathers, Wendy M Novicoff, Heather L Cox
Background: Nucleic acid microarray (NAM) testing for detection of Staphylococcus aureus bacteremia (SAB) and S. aureus resistance gene determinants can reduce time to targeted antibiotic administration. Evidence-based management of SAB includes bedside Infectious Diseases (ID) consultation. As a healthcare improvement initiative at our institution, with the goal of improving management and outcomes for subjects with SAB, we implemented NAM with a process for responding to positive NAM results by directly triggered, mandatory ID consultation...
August 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29020166/long-term-impact-of-an-educational-antimicrobial-stewardship-program-on-hospital-acquired-candidemia-and-multidrug-resistant-bloodstream-infections-a-quasi-experimental-study-of-interrupted-time-series-analysis
#17
José Molina, Germán Peñalva, María V Gil-Navarro, Julia Praena, José A Lepe, María A Pérez-Moreno, Carmen Ferrándiz, Teresa Aldabó, Manuela Aguilar, Peter Olbrich, Manuel E Jiménez-Mejías, María L Gascón, Rosario Amaya-Villar, Olaf Neth, María J Rodríguez-Hernández, Antonio Gutiérrez-Pizarraya, José Garnacho-Montero, Cristina Montero, Josefina Cano, Julián Palomino, Raquel Valencia, Rocío Álvarez, Elisa Cordero, Marta Herrero, José M Cisneros
Background: The global crisis of bacterial resistance urges the scientific community to implement intervention programs in healthcare facilities to promote an appropriate use of antibiotics. However, the clinical benefits or the impact on resistance of these interventions has not been definitively proven. Methods: We designed a quasi-experimental intervention study with an interrupted time-series analysis. A multidisciplinary team conducted a multifaceted educational intervention in our tertiary-care hospital over a five-year period...
August 7, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29020143/anti-infective-acquisition-costs-for-a-stewardship-program-getting-to-the-bottom-line
#18
Sarah K Parker, Amanda L Hurst, Cary Thurm, Matthew Millard, Timothy C Jenkins, Jason Child, Casey Dugan
Background: Though antimicrobial stewardship programs (ASPs) are in place for patient safety, financial justification is often required. In 2016, the Infectious Diseases Society of America (IDSA) recommended that anti-infective costs be measured by patient-level administration data normalized for patient census. Few publications use this methodology. Here, we aim to compare 3 methods of drug cost analysis during 3 phases of an ASP as an example of this recommendation's implementation...
July 23, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29019465/facile-and-controllable-electrochemical-fabrication-of-cell-adhesive-polypyrrole-electrodes-using-pyrrole-rgd-peptides
#19
Lindy K Jang, Semin Kim, Jiwon Seo, Jae Young Lee
Electrically conductive polymers, such as polypyrrole (PPy), have been widely used for the fabrication of various biosensors and tissue engineering scaffolds. For their biologically relevant applications, conductive biomaterials capable of intimate cellular interactions are highly desired. However, conventional methods to incorporate biomolecules into conductive polymers do not offer fine and easy control over the surface density of the biomolecules and/or their stability. We present a novel method to electrochemically immobilize cell adhesive Arg-Gly-Asp (RGD) ligands on PPy electrode surfaces with a simple control over the peptide surface density by varying the electrodeposition time...
October 11, 2017: Biofabrication
https://www.readbyqxmd.com/read/29018054/the-induction-of-myeloma-cell-death-and-dna-damage-by-tetrac-a-thyroid-hormone-derivative
#20
Keren Cohen, Uri Abadi, Aleck Hercbergs, Paul J Davis, Martin Ellis, Osnat Ashur-Fabian
Multiple myeloma (MM) is a plasma cell malignancy in which involvement of the thyroid hormone-integrin αvβ3 pathway was shown and pharmacologic inhibition of this pathway is a rational approach to disease management. A thyroid hormone derivative, tetraiodothyroacetic acid (tetrac), which inhibits L-thyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) binding to αvβ3 integrin, was studied in five MM cell lines and primary bone marrow (BM) MM cells. Tetrac inhibited MM cell proliferation (absolute cell number/viability) and induced caspase-dependent apoptosis (annexin-V/PI and cell cycle)...
October 10, 2017: Endocrine-related Cancer
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