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https://www.readbyqxmd.com/read/28820183/technique-of-retinal-gene-therapy-delivery-of-viral-vector-into-the-subretinal-space
#1
K Xue, M Groppe, A P Salvetti, R E MacLaren
PurposeSafe and reproducible delivery of gene therapy vector into the subretinal space is essential for successful targeting of the retinal pigment epithelium (RPE) and photoreceptors. The success of surgery is critical for the clinical efficacy of retinal gene therapy. Iatrogenic detachment of the degenerate (often adherent) retina in patients with hereditary retinal degenerations and small volume (eg, 0.1 ml) subretinal injections pose new surgical challenges.MethodsOur subretinal gene therapy technique involved pre-operative planning with optical coherence tomography (OCT) and autofluorescence (AF) imaging, 23 G pars plana vitrectomy, internal limiting membrane staining with Membrane Blue Dual (DORC BV, Zuidland, Netherlands), a two-step subretinal injection using a 41 G Teflon tipped cannula (DORC) first with normal saline to create a parafoveal bleb followed by slow infusion of viral vector via the same self-sealing retinotomy...
August 18, 2017: Eye
https://www.readbyqxmd.com/read/28810041/morphologic-and-postoperative-fixation-characteristics-of-the-macular-epiretinal-membrane-in-young-patients-undergoing-surgery
#2
Tian Tian, Chunli Chen, Haiying Jin, Qi Zhang, Yu Xu, Shiyuan Wang, Xiang Zhang, Peiquan Zhao
BACKGROUND AND OBJECTIVE: To describe morphologic characteristics, surgical outcomes, postoperative preferred retinal loci (PRL), and fixation stability of epiretinal membranes (ERMs) in young patients. PATIENTS AND METHODS: This retrospective study analyzed 43 eyes of 41 young patients (age range: 0.8 years to 28 years) with ERMs, which were divided into two groups: secondary ERMs (SERMs; n = 28) and idiopathic ERMs (IERMs; n = 13). All 43 eyes underwent 25-gauge vitrectomy and membrane peeling...
August 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28799561/retinal-detachment-repair-and-cataract-surgery-in-patients-with-atopic-dermatitis
#3
V Gnana Jothi, S McGimpsey, J A Sharkey, W C Chan
PurposeThe aim of this study is to report a case series of ocular complications including retinal detachment (RD) and cataract in atopic dermatitis (AD) and surgical management involving a majority of Caucasian patients.Patients and methodsThis study is an observational case series, originally designed as an audit. It involves detailed discussion of history, clinical features, and surgical management of patients presenting with retinal detachment and cataracts secondary to severe AD. Six consecutive patients with diagnosis of severe AD requiring posterior segment and cataract surgery were included in the study...
August 11, 2017: Eye
https://www.readbyqxmd.com/read/28796149/visual-and-anatomical-outcomes-after-diabetic-traction-and-traction-rhegmatogenous-retinal-detachment-repair
#4
Philip P Storey, Anna Ter-Zakarian, Shannon A Philander, Lisa Olmos de Koo, Meena George, Mark S Humayun, Damien C Rodger, Hossein Ameri
PURPOSE: To evaluate visual and anatomical outcomes of diabetic tractional retinal detachment repaired with pars plana vitrectomy. METHODS: Operative records were used to retrospectively identify all patients with tractional retinal detachments secondary to proliferative diabetic retinopathy surgically repaired with pars plana vitrectomy between November 1, 2009, and January 1, 2015 at the LAC + USC (Los Angeles County + University of Southern California) Medical Center...
August 8, 2017: Retina
https://www.readbyqxmd.com/read/28764684/intraoperative-optical-coherence-tomography-assisted-analysis-of-pars-plana-vitrectomy-for-retinal-detachment-in-morning-glory-syndrome-a-case-report
#5
Lyubomyr M Lytvynchuk, Carl G Glittenberg, Siamak Ansari-Shahrezaei, Susanne Binder
BACKGROUND: The pathogenesis of non-rhegmatogenous retinal detachment (non-RRD) associated with morning glory syndrome (MGS) is not established, as well as best surgical approach to treat RD. Our purpose was to analyse intraoperative optical coherence tomography data (iOCT) in all steps of pars plana vitrectomy (PPV) for non-RRD in MGS, in order to follow pathophysiological aspects of the disease and to understand the tissues behaviour during surgical workflow. CASE PRESENTATION: Intraoperative spectral domain optical coherent tomography (iSD-OCT) assisted PPV using Rescan 700 (Carl Zeiss Meditech, Jena, Germany) with epiretinal membrane (ERM) and internal retinal membrane (ILM) peeling, and air endotamponade was performed on the only eye of a 21 years old female with non-RRD associated with MGS...
August 1, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28744658/characteristics-of-retinal-vessels-in-surgically-closed-macular-hole-an-optical-coherence-tomography-angiography-study
#6
Cheolmin Yun, Jaemoon Ahn, Mingue Kim, Jee Taek Kim, Soon-Young Hwang, Seong-Woo Kim, Jaeryung Oh
PURPOSE: The purpose of our study was to investigate characteristics of retinal vessels in eyes with surgically closed macular holes (MH). METHODS: We included patients who underwent surgery for idiopathic MH and a follow-up examination using optical coherence tomography angiography (OCTA). The area of the foveal avascular zone (FAZ) and retinal vascular densities of the superficial capillary plexus (SCP) and deep capillary plexus (DCP) were calculated on the postoperative OCTA images and compared with those of age-matched normal controls...
July 25, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28719002/eyeslam-real-time-simultaneous-localization-and-mapping-of-retinal-vessels-during-intraocular-microsurgery
#7
Daniel Braun, Sungwook Yang, Joseph N Martel, Cameron N Riviere, Brian C Becker
BACKGROUND: Fast and accurate mapping and localization of the retinal vasculature is critical to increasing the effectiveness and clinical utility of robot-assisted intraocular microsurgery such as laser photocoagulation and retinal vessel cannulation. METHODS: The proposed EyeSLAM algorithm delivers 30 Hz real-time simultaneous localization and mapping of the human retina and vasculature during intraocular surgery, combining fast vessel detection with 2D scan-matching techniques to build and localize a probabilistic map of the vasculature...
July 18, 2017: International Journal of Medical Robotics + Computer Assisted Surgery: MRCAS
https://www.readbyqxmd.com/read/28677833/vitrectomy-with-a-modified-temporal-inverted-limiting-membrane-flap-to-reconstruct-the-foveolar-architecture-for-macular-hole-retinal-detachment-in-highly-myopic-eyes
#8
Tzyy-Chang Ho, Allen Ho, Muh-Shy Chen
PURPOSE: We investigated the surgical results of macular hole retinal detachment (MHRD) with a modified C-shaped temporal inverted internal limiting membrane (ILM) flap to reconstruct the foveolar architecture in highly myopic eyes. METHODS: Eighteen highly myopic eyes with MHRD in 17 patients who underwent a vitrectomy with a modified C-shaped temporal inverted ILM flap were followed for 12 months. Anatomic outcomes were evaluated by fundus examinations and optical coherence tomography...
July 5, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28676862/use-of-brilliant-blue-g-in-descemet-s-membrane-endothelial-keratoplasty
#9
Takahiko Hayashi, Kentaro Yuda, Itaru Oyakawa, Naoko Kato
Vital staining of the endothelial graft is essential during Descemet's membrane endothelial keratoplasty (DMEK) to ensure surgical success. DMEK surgeons worldwide commonly use trypan blue (TB) to this end. However, TB may exert toxic effects on both the cornea and retina. Recently, Brilliant Blue G (BBG) has become recognized as an alternative stain for use during vitreoretinal surgery; BBG is associated with lower levels of toxicity. We retrospectively analyzed the utility of BBG staining during DMEK. We used 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28657533/-the-ametrop%C3%A3-as-updated-review-for-non-ophthalmologists-physicians
#10
REVIEW
Virgilio Galvis, Alejandro Tello, Oscar Blanco, Andrea N Laiton, Marion R Dueñas, Priscila Alejandra Hidalgo
Refractive errors are caused by a decoupling of the power of convergence of the eye lens, the cornea and lens, which make the rays reaching the eye to focus and generate an image, and the retina, which is the biological photosensitive screen where the image will be transformed into a nerve impulse. These defects include myopia, hyperopia and astigmatism. Presbyopia can also be considered a refractive defect, but of special features, since only affects near vision in patients older than 40 years. By altering the quality of the focused image on the most sensitive area of the retina (the macula), they decrease visual acuity...
2017: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/28650920/intraoperative-imaging-of-retained-perfluorocarbon-liquid-using-spectral-domain-optical-coherence-tomography
#11
Margaret R Strampe, Mark M Kaehr, Joseph Carroll, Judy E Kim
PURPOSE: To report images of retained perfluorocarbon liquid (PFCL) on the surface of the retina obtained during an intraoperative use of hand-held spectral domain optical coherence tomography. METHODS: A 54-year-old man underwent pars plana vitrectomy with injection of PFCL to repair retinal detachment. Postoperatively, visually significant PFCL droplets were found to be retained in the eye. During the surgical removal of PFCL, a hand-held spectral domain optical coherence tomography was used to image the retina with the patient supine...
June 19, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28631180/microvascular-changes-after-vitrectomy-with-internal-limiting-membrane-peeling-an-optical-coherence-tomography-angiography-study
#12
Leonardo Mastropasqua, Enrico Borrelli, Paolo Carpineto, Lisa Toto, Luca Di Antonio, Peter A Mattei, Rodolfo Mastropasqua
PURPOSE: To evaluate superficial capillary plexus (SCP) changes occurring after internal limiting membrane (ILM) peeling for the treatment of idiopathic epiretinal membrane (ERM). METHODS: A total of 15 eyes of 15 patients affected by idiopathic ERM (eight males and seven females; mean age 59.8 ± 9.6 years) were enrolled in the study. Patients were treated with pars plana vitrectomy followed by ERM and ILM peeling. Subjects were evaluated at baseline and at the week-1 and month-1 follow-up visits...
June 19, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28629380/retinal-and-vitreous-metastases-from-hepatocholangiocarcinoma
#13
Anna Praidou, Sarita Jacob, Luciane Irion, Ramesh Sivaraj, Carl Groenewald, Sarah E Coupland, Heinrich Heimann
BACKGROUND: To report a case of metastatic hepatocholangiocarcinoma to the vitreous and retina. CASE PRESENTATION: A 70-year-old male, who was recently diagnosed with hepatocholangiocarcinoma, was complaining of floaters in his right eye over the past 5 months and was referred to the Liverpool Ocular Oncology Centre. On presentation, his visual acuity in the right eye was 6/24. Fundus exam revealed a whitish, unilateral, full-thickness retinal lesion at the inferotemporal arcade of his right eye, with vitreous infiltration and subretinal fluid...
June 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28611650/anterior-360%C3%A2-synechiolysis-in-a-case-of-late-iridocorneal-adhesions-after-25-g-vitrectomy-surgical-and-physiopathogenetic-aspects
#14
Raffaele Nuzzi, Francesca Monteu
We describe the case of an 86-year-old patient, pseudophakic in both eyes and with high myopia, who had previously had a 25-G vitrectomy with 20% C3F8 used as a tamponade due to a total retinal detachment with choroidal hemorrhages and macular hole. At the postoperative 4-month follow-up, we found 360° iridocorneal synechiae with elevated intraocular pressure due to angle closure in all sectors, with an adherent retina and in the absence of choroidal hemorrhage/detachment and of corneal edema or endothelial damage...
January 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28608271/repeated-subretinal-surgery-and-removal-of-subretinal-decalin-is-well-tolerated-evidence-from-a-porcine-model
#15
Nina Buus Sørensen, Kristian Klemp, Troels Wesenberg Kjær, Steffen Heegaard, Morten la Cour, Jens Folke Kiilgaard
PURPOSE: Subretinal perfluorocarbon liquid (PFCL) is a serious complication that can occur after retinal detachment repair. It is possible to remove the PFCL surgically, but retinal damage related to the procedure is unknown. Also, increasing interest in subretinal treatment makes it relevant to examine the functional and morphological consequences of repeated subretinal manipulation. We hypothesized that PFCL in a porcine model can be injected in the subretinal space and removed with minimal effect on retinal structure and function...
June 12, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28604979/femtosecond-laser-assisted-cataract-in-vitreoretinal-surgery
#16
Stanislao Rizzo, Daniela Bacherini, Francesco Faraldi, Marco Pellegrini, Cesare Mariotti
PURPOSE: To assess the safety and surgical results of femtosecond laser-assisted phacovitrectomy. METHODS: Fifteen patients over 50 years of age with coexisting vitreoretinal pathologies and cataract underwent combined femtosecond laser-assisted cataract extraction and sutureless 25-G and 27-G vitreoretinal surgery. RESULTS: The indication for surgery was macular hole in 6 patients, epiretinal membrane in 6, vitreous hemorrhage in 2, and retinal detachment in 1...
June 2, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28602014/correlation-between-morphological-changes-and-functional-outcomes-of-recent-onset-macula-off-rhegmatogenous-retinal-detachment-prognostic-factors-in-rhegmatogenous-retinal-detachment
#17
Murat Karacorlu, Isil Sayman Muslubas, Mumin Hocaoglu, Serra Arf, Mehmet Giray Ersoz
PURPOSE: To assess correlation between preoperative and postoperative findings and surgical factors, and postoperative functional outcomes after successful repair of acute macula-off rhegmatogenous retinal detachment (RRD). METHODS: Forty-four eyes of 44 patients with recent-onset macula-off RRD who had primary pars plana vitrectomy were included in this retrospective study. Best-corrected visual acuity (BCVA) and retinal and choroidal changes on spectral-domain optical coherence tomography (SD-OCT) were evaluated before and after surgery...
June 10, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28594752/residual-vitreous-cortex-at-the-fovea-during-vitrectomy-for-primary-rhegmatogenous-retinal-detachment-repair
#18
Eun Hyung Cho, Hee Chan Ku, Won Il, Eun Koo Lee
PURPOSE: To investigate the frequency of residual cortex and the effectiveness of removal of residual cortex in the fovea during vitrectomy for primary rhegmatogenous retinal detachment repair. METHODS: We retrospectively reviewed the charts and the operative videos of the 80 patients (80 eyes) who underwent vitrectomy for repair and who were observed for more than 6 months. After triamcinolone acetonide injection, we observed residual cortex on the surface of the retina...
June 7, 2017: Retina
https://www.readbyqxmd.com/read/28590320/sticky-silicone-oil
#19
Hamouda Hamdi Ghoraba, Adel Galal Zaky, Haithem Mamoun Abd Al Fatah, Emad El Din Mohamed El Gemai, Mohamed Amin Heikal
OBJECTIVE: To report and analyze the phenomenon of sticky silicone oil on the retina at the time of its removal. BACKGROUND: Silicone oil has been used for more than 25 years as a long-term retinal tamponade in the surgical management of patients with complex retinal detachments. When a decision to remove silicone oil was made at the end of the tamponade period, the system involved a number of different materials: silicone oil, aqueous solution with or without remnants of perfluorocarbon liquid plus the material of the cannula used to aspirate Silicone oil...
August 2017: Retina
https://www.readbyqxmd.com/read/28590319/surgical-outcomes-of-27-gauge-vitrectomy-for-a-consecutive-series-of-163-eyes-with-various-vitreous-diseases
#20
Kazuhito Yoneda, Keisuke Morikawa, Yusuke Oshima, Shigeru Kinoshita, Chie Sotozono
PURPOSE: To evaluate the safety and efficacy of 27-gauge vitrectomy for various vitreoretinal disorders. METHODS: In this retrospective comparative study, 163 consecutive eyes with various diseases that underwent 27-gauge pars plana vitrectomy with or without ultraspeed transformer by a single surgeon from June 2012 through December 2014 were analyzed in regard to best-corrected visual acuity, intraocular pressure, intraoperative and postoperative complications, and surgery time...
January 4, 2017: Retina
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