Sadis Matalon, Zhihong Yu, Shubham Dubey, Israr Ahmad, Emily M Stephens, Ammar Saadoon Alishlash, Ashley Meyers, Douglas Cossar, Donald Stewart, Edward P Acosta, Kyoko Kojima, Tamas Jilling, James A Mobley
We assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2 ) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acidosis, increased concentrations of plasma proteins in the alveolar space, lung mitochondrial DNA injury, increased levels of free plasma heme and major alterations of their lung proteome...
December 27, 2023: American Journal of Physiology. Lung Cellular and Molecular Physiology