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https://www.readbyqxmd.com/read/28449248/betanodavirus-dissection-of-the-viral-life-cycle
#1
REVIEW
C-F Low, B Syarul Nataqain, H-Y Chee, M Z H Rozaini, M Najiah
Progressive research has been recently made in dissecting the molecular biology of Betanodavirus life cycle, the causative pathogen of viral encephalopathy and retinopathy in economic important marine fish species. Establishment of betanodavirus infectious clone allows the manipulation of virus genome for functional genomic study, which elucidates the biological event of the viral life cycle at molecular level. The betanodavirus strategizes its replication by expressing anti-apoptosis/antinecrotic proteins to maintain the cell viability during early infection...
April 27, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28445005/proteomic-based-investigations-on-the-mode-of-action-of-the-marine-anticancer-compound-rhizochalinin
#2
Sergey A Dyshlovoy, Katharina Otte, Simone Venz, Jessica Hauschild, Heike Junker, Tatyana N Makarieva, Stefan Balabanov, Winfried H Alsdorf, Ramin Madanchi, Friedemann Honecker, Carsten Bokemeyer, Valentin A Stonik, Gunhild von Amsberg
Rhizochalinin (Rhiz) is a novel marine natural sphingolipid-like compound which shows promising in vitro and in vivo activity in human castration-resistant prostate cancer (CRPC). In the present study, a global proteome screening approach was applied to investigate molecular targets and biological processes affected by Rhiz in CRPC. Bioinformatical analysis of the data predicted an anti-migratory effect of Rhiz on cancer cells. Validation of proteins involved in the cancer-associated processes, including cell migration and invasion, revealed down-regulation of specific isoforms of stathmin and LASP1, as well as up-regulation of Grp75, keratin 81, and precursor IL-1β by Rhiz...
April 26, 2017: Proteomics
https://www.readbyqxmd.com/read/28441466/biomonitoring-of-marine-vertebrates-in-monterey-bay-using-edna-metabarcoding
#3
Elizabeth A Andruszkiewicz, Hilary A Starks, Francisco P Chavez, Lauren M Sassoubre, Barbara A Block, Alexandria B Boehm
Molecular analysis of environmental DNA (eDNA) can be used to assess vertebrate biodiversity in aquatic systems, but limited work has applied eDNA technologies to marine waters. Further, there is limited understanding of the spatial distribution of vertebrate eDNA in marine waters. Here, we use an eDNA metabarcoding approach to target and amplify a hypervariable region of the mitochondrial 12S rRNA gene to characterize vertebrate communities at 10 oceanographic stations spanning 45 km within the Monterey Bay National Marine Sanctuary (MBNMS)...
2017: PloS One
https://www.readbyqxmd.com/read/28441344/collagenous-extracellular-matrix-biomaterials-for-tissue-engineering-lessons-from-the-common-sea-urchin-tissue
#4
REVIEW
Kheng Lim Goh, David F Holmes
Scaffolds for tissue engineering application may be made from a collagenous extracellular matrix (ECM) of connective tissues because the ECM can mimic the functions of the target tissue. The primary sources of collagenous ECM material are calf skin and bone. However, these sources are associated with the risk of having bovine spongiform encephalopathy or transmissible spongiform encephalopathy. Alternative sources for collagenous ECM materials may be derived from livestock, e.g., pigs, and from marine animals, e...
April 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28440928/microplastics-as-contaminants-in-commercially-important-seafood-species
#5
David Santillo, Kathryn Miller, Paul Johnston
The ingestion of microplastic fragments, spheres, and fibers by marine mollusks, crustaceans, and fish, including a number of commercially important species, appears to be a widespread and pervasive phenomenon. Evidence is also growing for direct impacts of microplastic ingestion on physiology, reproductive success and survival of exposed marine organisms, and transfer through food webs, although the ecological implications are not yet known. Concerns also remain over the capacity for microplastics to act as vectors for harmful chemical pollutants, including plastic additives and persistent organic pollutants, although their contribution must be evaluated alongside other known sources...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28438338/effects-of-polychlorinated-biphenyls-on-metamorphosis-of-a-marine-fish-japanese-flounder-paralichthys-olivaceus-in-relation-to-thyroid-disruption
#6
Yifei Dong, Xiaona Zhang, Hua Tian, Xiang Li, Wei Wang, Shaoguo Ru
This study examined the influence of environmental concentrations of Aroclor 1254 (10, 100, and 1000ng/L) on metamorphosis of Paralichthys olivaceus, and analyzed the mechanisms in relation to thyroid disruption. Results showed that 100 and 1000ng/L Aroclor 1254 delayed metamorphosis and that 1000ng/L Aroclor 1254 caused abnormal morphology. Thyroxine and triiodothyronine levels in the control group were significantly elevated at metamorphic climax, but treatment with 100 and 1000ng/L delayed the increase in thyroid hormones (THs) and retarded metamorphic processes...
April 21, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28436509/covering-the-optical-spectrum-through-collective-rare-earth-doping-of-nagdf4-nanoparticles-806-and-980-nm-excitation-routes
#7
A Skripka, R Marin, A Benayas, P Canton, E Hemmer, F Vetrone
Today, at the frontier of biomedical research, the need has been clearly established for integrating disease detection and therapeutic function in one single theranostic system. Light-emitting nanoparticles are being intensively investigated to fulfil this demand, by continuously developing nanoparticle systems simultaneously emitting in both the UV/visible (light-triggered release and activation of drugs) and the near-infrared (imaging and tracking) spectral regions. In this work, rare-earth (RE) doped nanoparticles (RENPs) were synthesized via a thermal decomposition process and spectroscopically investigated as potential candidates as all-in-one optical imaging, diagnostic and therapeutic agents...
April 24, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28433779/identification-of-new-anti-inflammatory-agents-based-on-nitrosporeusine-natural-products-of-marine-origin
#8
Satish Chandra Philkhana, Abhishek Kumar Verma, Gorakhnath R Jachak, Bibhabasu Hazra, Anirban Basu, D Srinivasa Reddy
Nitrosporeusines A and B are two recently isolated marine natural products with novel skeleton and exceptional biological profile. Interesting antiviral activity of nitrosporeusines and promising potential in curing various diseases, evident from positive data from various animal models, led us to investigate their anti-inflammatory potential. Accordingly, we planned and synthesized nitrosporeusines A and B in racemic as well as enantiopure forms. The natural product synthesis was followed by preparation of several analogues, and all the synthesized compounds were evaluated for in vitro and in vivo anti-inflammatory potential...
April 13, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28432475/a-shinella-%C3%AE-n-acetylglucosaminidase-of-glycoside-hydrolase-family-20-displays-novel-biochemical-and-molecular-characteristics
#9
Junpei Zhou, Zhifeng Song, Rui Zhang, Caihong Chen, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han, Zunxi Huang
β-N-Acetylglucosaminidases (GlcNAcases) are important for many biological functions and industrial applications. In this study, a glycoside hydrolase family 20 GlcNAcase from Shinella sp. JB10 was expressed in Escherichia coli BL21 (DE3). Compared to many GlcNAcases, the purified recombinant enzyme (rJB10Nag) exhibited a higher specificity activity (538.8 µmol min(-1) mg(-1)) or V max (1030.0 ± 82.1 µmol min(-1) mg(-1)) toward p-nitrophenyl β-N-acetylglucosaminide and N,N'-diacetylchitobiose (specificity activity of 35...
April 21, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28431764/seasonal-monitoring-of-blue-mussel-mytilus-spp-populations-in-a-harbor-area-a-focus-on-responses-to-environmental-factors-and-chronic-contamination
#10
C Lacroix, E Duvieilbourg, N Guillou, J Guyomarch, C Bassoulet, D Moraga, G Chapalain, M Auffret
Coastal waters corresponding to macrotidal systems are among the most variable marine biotopes. Sessile animals as bivalve mollusks may however be found forming intertidal beds at high densities, as allowed by full adaptation to local conditions. A better knowledge of adaptive responses to environmental factors is required to foresee possible adverse effects of global change. At the sub-cellular level, transcriptional responses are among the earliest signals of environmental disturbances and they can reveal subtle and meaningful changes in organism exposed to stress...
April 12, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28430190/tuning-underwater-adhesion-with-cation-%C3%AF-interactions
#11
Matthew A Gebbie, Wei Wei, Alex M Schrader, Thomas R Cristiani, Howard A Dobbs, Matthew Idso, Bradley F Chmelka, J Herbert Waite, Jacob N Israelachvili
Cation-π interactions drive the self-assembly and cohesion of many biological molecules, including the adhesion proteins of several marine organisms. Although the origin of cation-π bonds in isolated pairs has been extensively studied, the energetics of cation-π-driven self-assembly in molecular films remains uncharted. Here we use nanoscale force measurements in combination with solid-state NMR spectroscopy to show that the cohesive properties of simple aromatic- and lysine-rich peptides rival those of the strong reversible intermolecular cohesion exhibited by adhesion proteins of marine mussel...
May 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28429758/intracellular-lipid-binding-protein-family-diversity-from-oyster-crassostrea-gigas-genomic-and-structural-features-of-invertebrate-lipid-transporters
#12
Guilherme de Toledo-Silva, Guilherme Razzera, Flavia Lucena Zacchi, Nestor Cubas Wendt, Jacó Joaquim Mattos, Afonso Celso Dias Bainy
Intracellular lipid binding proteins (iLBPs) play a role in the transport and cellular uptake of fatty acids and gene expression regulation. The aim of this work was to characterize the iLBP gene family of the Pacific oyster Crassostrea gigas, one of the most cultivated marine bivalves in the world, using bioinformatics and molecular biology approaches. A total of 26 different iLBPs transcripts were identified in the Pacific oyster genome, including alternative splicing and gene duplication events. The oyster iLBP gene family seems to be more expanded than in other invertebrates...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28429314/evolutionary-origin-of-euglena
#13
Bożena Zakryś, Rafał Milanowski, Anna Karnkowska
Euglenids (Excavata, Discoba, Euglenozoa, Euglenida) is a group of free-living, single-celled flagellates living in the aquatic environments. The uniting and unique morphological feature of euglenids is the presence of a cell covering called the pellicle. The morphology and organization of the pellicle correlate well with the mode of nutrition and cell movement. Euglenids exhibit diverse modes of nutrition, including phagotrophy and photosynthesis. Photosynthetic species (Euglenophyceae) constitute a single subclade within euglenids...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28425963/a-multi-platform-optical-sensor-for-in-vivo-and-in-vitro-algae-classification
#14
Chee-Loon Ng, Qing-Qing Chen, Jia-Jing Chua, Harold F Hemond
Differentiation among major algal groups is important for the ecological and biogeochemical characterization of water bodies, and for practical management of water resources. It helps to discern the taxonomic groups that are beneficial to aquatic life from the organisms causing harmful algal blooms. An LED-induced fluorescence (LEDIF) instrument capable of fluorescence, absorbance, and scattering measurements; is used for in vivo and in vitro identification and quantification of four algal groups found in freshwater and marine environments...
April 20, 2017: Sensors
https://www.readbyqxmd.com/read/28425091/marine-bioluminescence-measurement-by-a-classical-light-sensor-and-related-foraging-behaviour-of-a-deep-diving-predator
#15
Jade Vacquié-Garcia, Jérôme Mallefet, Frédéric Bailleul, Baptiste Picard, Christophe Guinet
Bioluminescence is produced by a broad range of organisms for defense, predation or communication purposes. Southern elephant seal (SES) vision is adapted to low intensity light with a peak sensitivity, matching the wavelength emitted by myctophid species, one of the main prey of female SES. A total of 11 satellite-tracked female SES were equipped with a Time-Depth-Light 3D-accelerometers (TDR10-X) in order to assess whether bioluminescence could be used by SES to locate their prey. First, we demonstrated experimentally that the TDR10-X light sensor was sensitive enough to detect natural bioluminescence; however, we highlighted a low distance detection of the sensor...
April 20, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28424665/ca-similichlamydia-in-epitheliocystis-co-infection-of-gilthead-seabream-gills-unique-morphological-features-of-a-deep-branching-chlamydial-family
#16
Helena M B Seth-Smith, Pantelis Katharios, Nancy Dourala, José M Mateos, Alexander G J Fehr, Lisbeth Nufer, Maja Ruetten, Maricruz Guevara Soto, Lloyd Vaughan
The Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) bacterial superphylum constitutes a broad range of organisms with an intriguing array of ultrastructural morphologies, including intracellular membranes and compartments and their corresponding complex genomes encoding these forms. The phylum Chlamydiae are all obligate intracellular bacteria and, although much is already known of their genomes from various families and how these regulate the various morphological forms, we know remarkably little about what is likely the deepest rooting clade of this phylum, which has only been found to contain pathogens of marine and fresh water vertebrates...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28424662/untangling-genomes-of-novel-planctomycetal-and-verrucomicrobial-species-from-monterey-bay-kelp-forest-metagenomes-by-refined-binning
#17
John Vollmers, Martinique Frentrup, Patrick Rast, Christian Jogler, Anne-Kristin Kaster
The kelp forest of the Pacific temperate rocky marine coastline of Monterey Bay in California is a dominant habitat for large brown macro-algae in the order of Laminariales. It is probably one of the most species-rich, structurally complex and productive ecosystems in temperate waters and well-studied in terms of trophic ecology. However, still little is known about the microorganisms thriving in this habitat. A growing body of evidence suggests that bacteria associated with macro-algae represent a huge and largely untapped resource of natural products with chemical structures that have been optimized by evolution for biological and ecological purposes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28424343/interactive-effects-of-temperature-food-and-skeletal-mineralogy-mediate-biological-responses-to-ocean-acidification-in-a-widely-distributed-bryozoan
#18
Daniel S Swezey, Jessica R Bean, Aaron T Ninokawa, Tessa M Hill, Brian Gaylord, Eric Sanford
Marine invertebrates with skeletons made of high-magnesium calcite may be especially susceptible to ocean acidification (OA) due to the elevated solubility of this form of calcium carbonate. However, skeletal composition can vary plastically within some species, and it is largely unknown how concurrent changes in multiple oceanographic parameters will interact to affect skeletal mineralogy, growth and vulnerability to future OA. We explored these interactive effects by culturing genetic clones of the bryozoan Jellyella tuberculata (formerly Membranipora tuberculata) under factorial combinations of dissolved carbon dioxide (CO2), temperature and food concentrations...
April 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28422974/variation-in-benthic-long-term-data-of-transitional-waters-is-interpretation-more-than-speculation
#19
Michael Lothar Zettler, René Friedland, Mayya Gogina, Alexander Darr
Biological long-term data series in marine habitats are often used to identify anthropogenic impacts on the environment or climate induced regime shifts. However, particularly in transitional waters, environmental properties like water mass dynamics, salinity variability and the occurrence of oxygen minima not necessarily caused by either human activities or climate change can attenuate or mask apparent signals. At first glance it very often seems impossible to interpret the strong fluctuations of e.g. abundances or species richness, since abiotic variables like salinity and oxygen content vary simultaneously as well as in apparently erratic ways...
2017: PloS One
https://www.readbyqxmd.com/read/28422340/leptolyngbyolides-cytotoxic-macrolides-from-the-marine-cyanobacterium-leptolyngbya-sp-isolation-biological-activity-and-catalytic-asymmetric-total-synthesis
#20
Jin Cui, Maho Morita, Osamu Ohno, Tomoyuki Kimura, Toshiaki Teruya, Takumi Watanabe, Kiyotake Suenaga, Masakatsu Shibasaki
Four new macrolactones, leptolyngbyolides A-D, were isolated from the cyanobacterium Leptolyngbya sp. collected in Okinawa, Japan. The planar structure of leptolyngbyolides were determined by extensive NMR studies, although complete assignment of the absolute configuration awaited the catalytic asymmetric total synthesis of leptolyngbyolide C. The synthesis took advantage of the catalytic asymmetric thioamide-aldol reaction using copper (I) complexed with a chiral bidentate phosphine ligand to regulate two key stereochemistries of the molecule at the outset...
April 19, 2017: Chemistry: a European Journal
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