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Susheela D Biranjia-Hurdoyal, Sharmila P Seetulsingh-Goorah
: The aim was to determine the performances of four Helicobacter pylori serological detection kits in different target groups, using Amplified IDEIA™ Hp StAR™ as gold standard. Kits studied were Rapid Immunochromatoghraphic Hexagon, Helicoblot 2.1, an EIA IgG kit and EIA IgA kit. METHODS: Stool and blood samples were collected from 162 apparently healthy participants (control) and 60 Type 2 diabetes mellitus (T2DM) patients. RESULTS: The performances of the four serological detection kits were found to be affected by gender, age, health status and ethnicity of the participants...
2016: PloS One
Thatianny Tanferri de Brito Paranaguá, Ana Lúcia Queiroz Bezerra, Gabriela Camargo Tobias, Suely Itsuko Ciosak
OBJECTIVE: to analyze the support for learning, in the perspective of patient safety, offered in the work environment, according to health professionals working in primary care. METHOD: a transversal study, held with 86 health professionals working in primary care. A validated instrument was used, applied via the Internet. Descriptive statistical analysis was undertaken with a presentation of median, mean, standard deviation and coefficient of variation. RESULTS: points which are favorable to supporting learning were evidenced, such as mutual respect, autonomy for organizing the work and valorization of new ideas, which obtained means above 7...
2016: Revista Latino-americana de Enfermagem
Ingrid Margareth Voth Lowen, Aida Maris Peres, Karla Crozeta, Elizabeth Bernardino, Carmem Lúcia Colomé Beck
OBJECTIVE: To relate the managerial competencies required of nurses with the process of change experienced in the expansion of the Family Health Strategy (FHS). METHOD: A qualitative research conducted in primary health care in a southern Brazilian city, through interviews with 32 managerial and clinical nurses. The interviews were processed by IRAMUTEQ software. The resulting classes were examined under five managerial competencies to promote change. RESULTS: The four classes obtained from data were: the Family Health Strategy expansion process; confrontations and potentialities; mobilization for the change; innovations in medical and nursing consultations...
December 2015: Revista da Escola de Enfermagem da U S P
Izabella Łazowska-Przeorek, Maria Kotowska, Marcin Banasiuk, Katarzyna Karolewska-Bochenek, Aleksandra Banaszkiewicz, Agnieszka Gawrońska, Piotr Albrecht
BACKGROUND: The aim of this study was to confirm the role of antral nodularity in the diagnosis of Helicobacter pylori (H. pylori) infection in children. MATERIAL AND METHODS: This prospective study included 107 children (58 male; 54.2%), between the ages of 3 and 18 years, infected with H. pylori, which was confirmed if the patient had at least 2 of 4 positive test results (urea breath test, urease test in gastric biopsy, histopathology - positive hematoxylin and eosin and Giemsa staining, and/or monoclonal stool ELISA test - Amplified IDEIA™ Hp StAR™)...
2015: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Priscila Queiroz D'Elia, Aline Amaro dos Santos, Bianca Bianco, Caio Parente Barbosa, Denise Maria Christofolini, Tsutomu Aoki
The aim of this study was to investigate the association between MTHFR gene polymorphisms and IVF outcomes in Brazilian women undergoing assisted reproduction treatment. A prospective study was conducted in the Human Reproduction Department at the ABC University School of Medicine and the Ideia Fertility Institute between December 2010 and April 2012. The patient population was 82 women undergoing assisted reproduction cycles. The MTHFR polymorphisms C677T and A1298C were evaluated and compared with laboratory results and pregnancy rates...
June 2014: Reproductive Biomedicine Online
A J Henningsson, M Christiansson, I Tjernberg, S Löfgren, A Matussek
The diagnosis of Lyme neuroborreliosis (LNB) requires the detection of intrathecal synthesis of Borrelia-specific antibodies, but in very early disease, the sensitivity may be low. We compared the performance of the second-generation IDEIA Lyme Neuroborreliosis test (Oxoid), based on purified native flagellum antigen, with two newly developed tests based on several recombinant antigens for the diagnosis of LNB. Patients investigated for LNB during 2003 through 2007 were included (n = 175); 52 with definite LNB, four with possible LNB and 119 non-LNB patients...
May 2014: European Journal of Clinical Microbiology & Infectious Diseases
Masoumeh Douraghi, Mahmoud Nateghi Rostami, Hossein Goudarzi, Zohreh Ghalavand
Diagnosis of active Helicobacter pylori infection in intellectually disabled (ID) children is problematic because they are unable to cooperate with performance of invasive tests. In this study, the non-invasive methods of measuring serum IgG antibody concentrations and performing stool antigen tests were used to screen for H. pylori infection in ID children. Eighty-seven children with intellectual disabilities were studied. The amount of serum IgG antibody against H. pylori was measured by the ELISA method...
November 2013: Microbiology and Immunology
Ram Benny Dessau
Two assays (Liaison, Diasorin; IDEIA, Oxoid) for detection of Borrelia-specific antibodies were compared. A case-control design using patients with neuroborreliosis (n = 48), laboratory defined by a positive Borrelia-specific antibody index in the spinal fluid, was available and was intended to represent the serological response of disseminated early Lyme borreliosis in general. Serum samples were obtained from 216 Danish blood donors as controls. By comparing sensitivity and specificity using pre-specified cut-off values, significant differences were found...
December 2013: Journal of Medical Microbiology
Doris Adams Hill, Regina Kearley
The diagnosis of autism spectrum disorder has systematically risen since Kanner's description in 1943 and Asperger's definition in 1944. An increase in numbers has met with an increase in litigation regarding autism spectrum disorder (ASD) and the Individuals with Disabilities Education Improvement Act (IDEIA). Outcomes that first favored parents (2002-2004) have moved to outcomes favoring school districts. The authors update the reader on case outcomes for 2010 and discuss how pending changes in legislation and diagnostic criteria may impact navigation through the education system as individuals seek a free appropriate public education (FAPE) and placement in the least restrictive environment (LRE)...
May 2013: Research in Developmental Disabilities
Zhong Yu Li, Qiu Lin Huang, Sheng Mei Su, Guang Ming Zhong, Yi Mou Wu
OBJECTIVE: To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections. METHODS: The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polyclonal antibody (pAb) for DAS-ELISAs. Clinical samples from 186 urogenital infection patients (groups I) and 62 healthy donors (groups II) were detected in parallel by the DAS-ELISAs developed in this study and by IDEIA PCE commercial ELISA...
March 2013: Biomedical and Environmental Sciences: BES
Robert D Inman, Basil Chiu
INTRODUCTION: Effective treatment of reactive arthritis would ideally achieve both control of inflammation and eradication of persisting arthritogenic pathogens. We use a model of experimental Chlamydia trachomatis-induced arthritis (CtIA) to evaluate the effectiveness of nafamostat mesilate (NM), a serine protease inhibitor with complement-modifying effects and anticoagulant properties. To date clinical use of NM has largely been in Asia and has been primarily confined to inflammatory states such as pancreatitis...
2012: Arthritis Research & Therapy
Céline Chappé, Sophie Minjolle, Alain Dabadie, Laetitia Morel, Ronald Colimon, Patrick Pladys
AIM: To describe clinical signs associated with Human Astrovirus (HAstV) in stools in neonatal units. METHODS: During 2005-2006, all stool virology performed for isolated digestive symptoms or suspicion of neonatal infection was tested for HAstV by an amplified enzyme-linked immunoassay (IDEIA™ Astrovirus test, Dako Cytomation). Each newborn with a positive result (HAstV+ group) was retrospectively matched with the first following symptomatic newborn in the same care unit having a negative stool virology (HAstV- group)...
May 2012: Acta Paediatrica
Beatrix Kele, György Lengyel, Judit Deak
Noroviruses are uncultivable; ELISA and reverse transcription polymerase chain reaction (RT-PCR) methods are therefore widely used for their detection. Sixty-one sporadic, diarrhoeal stool samples from various university hospital wards and from outpatients in Szeged, Hungary, were examined. Three methods were compared: two RT-PCR methods (the Argene Calici/Astrovirus Consensus kit and the Cepheid Norovirus Primer and Probe Set) and one ELISA method (the IDEIA™ Norovirus ELISA Test). Sensitivities of 78.9%, 92...
August 2011: Diagnostic Microbiology and Infectious Disease
Ila Geigenfeind, Daniel Haag-Wackernagel
Chlamydophila psittaci (Lillie, 1930) Everett et al., 1999, the pathogenic agent of human ornithosis, is widespread in feral pigeon populations and many cases of transmission from feral pigeons to humans have been reported. The aim of the present study was to detect C. psittaci in environmental samples to find out more about possible transmission routes and, therefore, to assess the zoonotic risk for humans. Fecal samples were collected from nest boxes in a feral pigeon loft. Additionally, samples were taken from the feather dust film covering the water surface of public fountains where pigeons regularly bathe...
March 2010: Integrative Zoology
Eric Wobudeya, Hanifa Bachou, Charles K Karamagi, Joan N Kalyango, Edrisa Mutebi, Henry Wamani
BACKGROUND: Rotavirus is responsible for over 25 million outpatient visits, over 2 million hospitalizations and 527,000 deaths annually, worldwide. It is estimated that breastfeeding in accordance with the World Health Organization recommendations would save 1.45 million children's lives each year in the developing countries. The few studies that examined the effect of breastfeeding on rotavirus diarrhea produced conflicting results. This study aimed to determine the effect of breastfeeding on rotavirus diarrhea among admitted infants in Uganda...
2011: BMC Pediatrics
P Pazdiora, J Táborska, M Svecova, J Sýkora
BACKGROUND: Norovirus-associated sporadic gastroenteritis is an important cause of illness in Western Europe. However, at present, little information on the role of norovirus in sporadic gastroenteritis in Central Europe is available. Our study aimed at providing an assessment of their significance in hospitalized children and adolescents with acute gastroenteritis using ELISA test at the time of their introduction. METHODS: A prospective hospital based study of the etiology of acute gastroenteritis was undertaken in a total of 618 patients (mean age 39...
November 2009: Epidemiologie, Mikrobiologie, Imunologie
Juan E Jiménez
Until recently, in the United States, the traditional way to identify students with Specific Learning Disabilities (SLD) was through the discrepancy model where student IQs were compared to their level of achievement. However, educators and researchers alike have questioned this model as a means to define and identify students with SLD. The 2004 reauthorization of the Individuals with Disabilities Education Improvement Act (IDEIA) includes the use of response to intervention (RtI) as possible alternative to the intelligence-achievement discrepancy for identifying SLD...
November 2010: Psicothema
Jane S Nakawesi, Eric Wobudeya, Grace Ndeezi, Edison A Mworozi, James K Tumwine
BACKGROUND: Rotavirus remains the commonest cause of severe dehydrating diarrhea among children worldwide. Children in developing countries die more because of several factors including poorer access to hydration therapy and greater prevalence of malnutrition. Hitherto, the magnitude of rotavirus disease in Uganda has remained unknown. This study was therefore done to determine the prevalence and factors associated with rotavirus infection among children aged 3-59 months admitted with acute diarrhea to paediatric emergency ward of Mulago Hospital, Uganda METHODS: Three hundred and ninety children, aged between 3-59 months with acute diarrhoea were recruited...
2010: BMC Pediatrics
Andrew Kirby, Ricardo Q Gurgel, Winifred Dove, Sarah Cristina F Vieira, Nigel A Cunliffe, Luis E Cuevas
BACKGROUND: The detection of norovirus by ELISA and immunochromatographic methods may facilitate epidemiological studies into the global disease burden associated with norovirus gastroenteritis and provide a quick method of testing for norovirus infection. OBJECTIVES: To evaluate the new RIDASCREEN norovirus ELISA (3rd generation) and RIDAQUICK norovirus immunochromatographic test on a collection of samples from Brazilian children with acute gastroenteritis, and compare them against the established 2nd generation IDEIA norovirus assay...
December 2010: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
J P Kabue, I Peenze, M de Beer, M D Esona, C Lunfungula, M Biamungu, T R Simba, J J Muyembe Tamfum, A Duncan Steele
Between July and October of 2003, 2004, and 2005, outbreaks of acute gastroenteritis occurred among children <5 years of age in Kinshasa, Democratic Republic of the Congo. Stool specimens (67 in 2003, 108 in 2004, and 116 in 2005) were collected and screened for rotaviruses using either latex agglutination (Diarlex LAA; Orion Diagnostics) or enzyme immunoassay (IDEIA; DakoCytomation). The molecular characteristics of the rotavirus strains were then determined. Group A rotavirus was detected in 195 (76%) of 258 stool specimens...
September 1, 2010: Journal of Infectious Diseases
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