Francisco A Venegas, Gabriele Köllisch, Kerstin Mark, Wibke E Diederich, Andreas Kaufmann, Stefan Bauer, Max Chavarría, Juan J Araya, Alfonso J García-Piñeres
Violacein, an indole-derived, purple-colored natural pigment isolated from Chromobacterium violaceum has shown multiple biological activities. In this work, we studied the effect of violacein in different immune cell lines, namely THP-1, MonoMac 6, ANA-1, Raw 264.7 cells, as well as in human peripheral blood mononuclear cells (PBMCs). A stimulation of TNF-α production was observed in murine macrophages (ANA-1 and Raw 264.7), and in PBMCs, IL-6 and IL-1β secretion was detected. We obtained evidence of the molecular mechanism of activation by determining the mRNA expression pattern upon treatment with violacein in Raw 264...
September 20, 2019: Scientific Reports