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Regenerative endodontic

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https://www.readbyqxmd.com/read/29662262/treatment-of-endo-periodontal-lesion-using-leukocyte-platelet-rich-fibrin-a-case-report
#1
Pablo Betancourt, Ricardo Elgueta, Ramon Fuentes
Case Description: The main objective of this paper was to report the clinical effectiveness of leukocyte- platelet- rich fibrin (L-PRF) in the treatment of a combined endo-periodontal lesion of an upper first premolar. Clinical Findings: The tooth had a profound abfraction on the vestibular aspect and presented no mobility but revealed a deep pocket measuring of 11 mm on the mesial vestibular aspect and 14 mm on the mesial palatine aspect. The three dimensional image analysis showed total bone loss in the mesial aspect and an extensively bone loss of the vestibular aspect of the vestibular root...
December 30, 2017: Colombia Médica: CM
https://www.readbyqxmd.com/read/29607842/a-clinicoradiographic-comparison-of-the-effects-of-platelet-rich-fibrin-gel-and-platelet-rich-fibrin-membrane-as-scaffolds-in-the-apexification-treatment-of-young-permanent-teeth
#2
Madhu Santhakumar, Shivsankar Yayathi, N Retnakumari
Aims: This triple blinded clinical trial was undertaken to check whether Platelet rich firin in its membrane form is as reliable as when it is in the gel form. Settings and Design: Triple blinded randomized clinical trial. Methods and Material: 20 patients in PRF gel group and 20 patients in PRF membrane group selected after randomization and considering inclusion and exclusion criteria were taken up for this study. The samples were clinically and radiographically evaluated for success...
January 2018: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/29581901/regenerative-endodontic-treatment-of-a-maxillary-mature-premolar
#3
Qingan Xu, Zhou Li
Regenerative endodontic treatment was performed on a mature maxillary premolar diagnosed as chronic pulpitis. The root canals were chemomechanically prepared and placed intracanal medicaments at the first appointment. Then 2 weeks later, a blood clot was created in the canals, over which mineral trioxide aggregate was placed. At 6-month follow-up, cementum-like tissue seemed to be formed in the root canal along with nearly recovered pulp vitality. At 12-month recall, the radiographic results revealed evidence of root wall thickening...
2018: Case Reports in Dentistry
https://www.readbyqxmd.com/read/29580724/application-of-enamel-matrix-derivative-emdogain-in-endodontic-therapy-a-comprehensive-literature-review
#4
REVIEW
Howard H Wang, Nima D Sarmast, Elham Shadmehr, Nikola Angelov, Shahrokh Shabahang, Mahmoud Torabinejad
INTRODUCTION: Enamel matrix derivative (EMD) is a protein extract used for the treatment of periodontal defects and soft tissue recession. Its use in endodontics has been a subject of exploration, especially in regenerative procedures. The aim of this review was to evaluate the current literature available on the application of EMD in the field of endodontics. METHODS: An initial literature search of databases using different combinations of the search terms yielded 1089 articles...
March 23, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29550003/dynamic-irrigation-promotes-apical-papilla-cell-attachment-in-an-ex-vivo-immature-root-canal-model
#5
Sanupong Prompreecha, Thanapat Sastraruji, Phumisak Louwakul, Tanida Srisuwan
INTRODUCTION: The use of dentin preconditioning techniques in regenerative endodontic procedures is currently promising. Several growth factors have been detected on dentin after ultrasonic irrigation with EDTA. This study aimed to evaluate the effects of dynamic irrigation with different solution regimens on apical papilla cell (APC) attachment in an ex vivo immature tooth model. METHODS: Various dynamic irrigation techniques, needle irrigation (NI), NI with EndoActivator, and NI with passive ultrasonic irrigation, were used with different solution regimens, normal saline solution (NSS), EDTA, and chlorhexidine digluconate followed by EDTA, in enlarged root canal models where calcium hydroxide-medicated dentin slices were inserted...
March 14, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29523112/do-hypoxia-and-l-mimosine-modulate-sclerostin-and-dickkopf-1-production-in-human-dental-pulp-derived-cells-insights-from-monolayer-spheroid-and-tooth-slice-cultures
#6
Klara Janjić, Barbara Cvikl, Christoph Kurzmann, Andreas Moritz, Hermann Agis
BACKGROUND: To understand the responses of the dental pulp to hypoxia is of high relevance for regenerative endodontics and dental traumatology. Here, we aimed to reveal the effects of hypoxia and the hypoxia mimetic agent L-mimosine (L-MIM) on the production of sclerostin (SOST) and dickkopf-1 (DKK-1) in human dental pulp-derived cells (DPC). METHODS: DPC in monolayer, spheroid and tooth slice cultures were treated with L-MIM or hypoxia. Resazurin-based toxicity and MTT assays were performed to determine cell viability...
March 9, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29480932/effectiveness-and-clinical-implications-of-the-use-of-topical-antibiotics-in-regenerative-endodontic-procedures-a-review
#7
REVIEW
P Montero-Miralles, J Martín-González, O Alonso-Ezpeleta, M C Jiménez-Sánchez, E Velasco-Ortega, J J Segura-Egea
Regenerative endodontic procedures (REPs) are biologically based procedures planned to replace damaged tissues, including dentinee and root structures, as well as cells of the pulp-dentine complex. Effective sterilization of the root canal is essential in REPs, and antibiotics have been widely used to disinfect root canals. The aim of this paper was to review the scientific literature on 1) Effectiveness of antibiotics used in REPs against bacteria implicated in endodontic disease; 2) Scientific evidence supporting the use of topical antibiotics in REPs; 3) Clinical implications of the use of antibiotics in REPs and the possible side effects; 4) Effect of antibiotics on dental pulp stem cells; and 5) Ongoing research on the use of antibiotics in REPs...
February 26, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29472688/regenerative-endodontic-therapy-for-managing-immature-non-vital-teeth-a-national-survey-of-uk-paediatric-dental-specialists-and-trainees
#8
H Nazzal, H Tong, P Nixon, M Duggal
Background Several guidelines have been published advocating the use of regenerative endodontic therapy (RET) in managing non-vital immature permanent teeth. It is unclear, however, how many UK paediatric dental specialists routinely use this technique and their opinion of its outcomes, and barriers to its use.Aim To assess the knowledge, experience and the opinion of UK based paediatric dental specialists/trainees (UKPDS/T) and practitioners working in the capacity of paediatric dental specialists on the use of RET...
February 23, 2018: British Dental Journal
https://www.readbyqxmd.com/read/29440793/use-of-an-amniotic-membrane-as-a-novel-barrier-in-a-tooth-with-a-questionable-prognosis
#9
Kanakamedala Anil Kumar, Muppalla Chakravarthy, Shalini Selvarajan, Theyagarajan Ramakrishnan, Geetha Ari
The chief goal of combination therapy using guided tissue regeneration along with bone grafts is to optimally and predictably regenerate the periodontal attachment apparatus. The evolution of regenerative therapy along with careful patient selection and treatment planning can have positive results even in cases which are not ideal. The present case report describes a tooth with a questionable prognosis treated successively by endodontic then periodontal therapy using an amniotic membrane along with a bone graft to treat an intrabony defect...
May 2017: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29399304/the-effect-of-photobiomodulation-on-the-depth-of-anesthesia-during-endodontic-treatment-of-teeth-with-symptomatic-irreversible-pulpitis-double-blind-randomized-clinical-trial
#10
Sholeh Ghabraei, Nasim Chiniforush, Behnam Bolhari, Mohsen Aminsobhani, Abbas Khosarvi
Introduction: Achieving appropriate anesthesia in patients with symptomatic irreversible pulpitis in mandibular molars during endodontic treatment is always one of the most challenging aspects. Photobiomodulation (PBM) has been used in dentistry due to its anti-inflammatory properties and regenerative effects. This study evaluates the effects of PBM in the depth of anesthesia in inferior alveolar nerve block. Methods: In this randomized clinical trial, 44 patients requiring endodontic treatment in lower molar, left or right were selected, half of them were randomly treated with PBM therapy...
2018: Journal of Lasers in Medical Sciences
https://www.readbyqxmd.com/read/29386790/use-of-bovine-colostrum-in-periapical-defects-following-surgical-endodontics-two-case-reports
#11
Chandrasekhar Veeramachaneni, Ch Gayathri, Abhijeet K Kakani, R Mohini
Periapical lesions are the most common pathological conditions involving teeth which often require surgical intervention. To achieve optimal healing both clinically and radiographically, bone grafts and barrier membranes have been used to fill the periapical defect after degranulation of the lesion. Colostrum is one of the new materials, which has osteoinductive or regenerative potential. The present case reports describe the use of bovine colostrum in the treatment of periapical inflammatory lesion with a follow-up period of 12 months...
September 2017: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/29336522/continued-root-maturation-despite-persistent-apical-periodontitis-of-immature-permanent-teeth-after-failed-regenerative-endodontic-therapy
#12
Louis M Lin, Sahng G Kim, Gabriela Martin, Bill Kahler
Three immature permanent teeth with pulp necrosis and apical periodontitis were treated with regenerative endodontic therapy (RET), which included root canal disinfection with sodium hypochlorite irrigation, intra-canal medication with calcium hydroxide paste, 17% EDTA rinse, induction of periapical bleeding into the canal, collagen matrix and MTA coronal seal, and composite resin restoration of access cavities. After different periods of follow-up, it was observed that continued root maturation, especially apical closure occurred despite persistent apical periodontitis of immature permanent teeth after failed RET...
January 16, 2018: Australian Endodontic Journal: the Journal of the Australian Society of Endodontology Inc
https://www.readbyqxmd.com/read/29306537/metformin-enhances-the-differentiation-of-dental-pulp-cells-into-odontoblasts-by-activating-ampk-signaling
#13
Wei Qin, Xianling Gao, Tao Ma, Michael D Weir, Jing Zou, Bing Song, Zhengmei Lin, Abraham Schneider, Hockin H K Xu
INTRODUCTION: Metformin is a first-line drug for treating type 2 diabetes that regulates the differentiation of mesenchymal stem cells. Its effects on human dental pulp cells (DPCs) remain unknown. This study aimed to investigate the effects of metformin on the proliferation and differentiation of DPCs. METHODS: A live/dead viability assay kit was used to examine the effects of metformin on the cell viability of DPCs. Cell proliferation was analyzed using a cell counting kit (CCK-8; Dojindo, Tokyo, Japan)...
April 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29306536/orthodontic-movement-after-regenerative-endodontic-procedure-case-report-and-long-term-observations
#14
Antonis Chaniotis
INTRODUCTION: Although regenerative treatment approaches in teeth with incomplete root formation and pulp necrosis have become part of the suggested therapeutic endodontic spectrum, little is known about the effect of orthodontic movement in the tissue that has been regenerated. Furthermore, as the number of adults undergoing orthodontic treatment increases, there is an increasing need to investigate the changes that these tissues may undergo during orthodontic movement. Here we describe the alterations observed after the application of orthodontic forces in a case of an apically root-fractured necrotic immature root that had been managed with regenerative endodontic procedures in the past...
March 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29275851/biocompatibility-and-osteogenic-calcification-potential-of-casein-phosphopeptide-amorphous-calcium-phosphate-fluoride
#15
Alaa E Dawood, David J Manton, Peter Parashos, Rebecca H Wong, William Singleton, James A Holden, Neil M O'Brien-Simpson, Eric C Reynolds
INTRODUCTION: Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and CPP-ACP with fluoride (CPP-ACFP) have been shown to provide bioavailable ions to promote mineralization. Hence, the aim of this study was to evaluate the materials' biocompatibility and osteogenic/calcification potential for endodontic applications. METHODS: Human and mouse osteoblast-like and fibroblast-like cell lines were incubated with 0.05%-3.0% w/v CPP-ACP and CPP-ACFP, and toxicity, proliferation, alkaline phosphatase, interleukin (IL)-1α, and IL-6 production, collagen type I, osteocalcin, and osteopontin production, and mineralization/calcification were determined...
March 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29275849/management-of-intruded-immature-maxillary-central-incisor-with-pulp-necrosis-and-severe-external-resorption-by-regenerative-approach
#16
Giorgos N Tzanetakis
INTRODUCTION: Intrusive luxation is one of the most severe traumatic injuries of permanent teeth that may adversely affect the pulp and the periodontium. Pulp necrosis and root resorption are the main pathologic entities associated with this injury. The present report describes the endodontic management of an intruded immature maxillary central incisor presented with pulp necrosis and severe inflammatory root resorption by using the regenerative approach. METHODS: A 7-year-old boy with dental trauma to the anterior maxillary region was referred for the management of a traumatized maxillary central incisor...
February 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29273495/influence-of-apical-diameter-on-the-outcome-of-regenerative-endodontic-treatment-in-teeth-with-pulp-necrosis-a-review
#17
REVIEW
Yanjun Fang, Xinhuan Wang, Jingjing Zhu, Chaonan Su, Ying Yang, Liuyan Meng
INTRODUCTION: The aim of this review was to evaluate whether the apical diameter of teeth with necrotic pulp affects the outcomes of regenerative endodontic treatment and to determine the minimal apical size needed to obtain proper pulp revascularization. METHODS: A literature search was performed from January 1, 2001, to November 25, 2016. Studies that satisfied the inclusion criteria were subjected to data extraction and analysis. RESULTS: In total, 14 studies with 85 patients were included...
March 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29229459/regenerative-endodontic-procedures-among-endodontists-a-web-based-survey
#18
Jonathan Y Lee, Daniel D Kersten, Pete Mines, Thomas A Beltran
INTRODUCTION: The protocols that endodontists implement for regenerative endodontic procedures (REPs) are unknown. The aim of this study was to examine current REP protocols among practicing endodontists in the United States. METHODS: A Web-based survey was sent to 4060 active members of the American Association of Endodontists (AAE). A total of 850 participants completed the survey, representing a 20.9% response rate. RESULTS: Responses indicated 60% reported having performed REPs; most performed 1 to 3 per year...
February 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29206139/alkaline-materials-and-regenerative-endodontics-a-review
#19
REVIEW
Bill Kahler, Nadia Chugal, Louis M Lin
Periapical health is the primary goal of endodontic treatment in mature and immature teeth. In addition, the goals of treatment of immature teeth with arrested root development include root growth to length and maturation of the apex, as well as thickening of the canal wall. These goals are valid for immature teeth that have been subjected to trauma and dental caries or that are the result of developmental anomalies that expose the tooth to the risk of pulp necrosis and consequently result in the cessation of root maturation...
December 5, 2017: Materials
https://www.readbyqxmd.com/read/29197101/a-biocompatible-decellularized-pulp-scaffold-for-regenerative-endodontics
#20
M Matoug-Elwerfelli, M S Duggal, H Nazzal, F Esteves, E Raïf
AIM: To investigate the feasibility of decellularizing the entire dental pulp using a mild treatment protocol to develop a decellularized biological extracellular matrix scaffold for use in regenerative endodontic procedures. METHODOLOGY: Decellularized human dental pulps were assessed using histological and immunohistochemical methods, scanning electron microscope and DNA quantification assay. Cytotoxicity assays to determine decellularized scaffold biocompatibility were also performed...
December 1, 2017: International Endodontic Journal
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