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human papilloma virus DNA

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https://www.readbyqxmd.com/read/29790789/assessment-of-the-association-between-the-frequency-of-micronucleus-and-p-16ink4a-ki-67-co-expression-in-patients-with-cervical-intraepithelial-lesions
#1
G Gashi, V Mahovlić, E Bahtiri, F Kurshumliu, A Podrimaj-Bytyqi, I R Elezaj
Human papilloma virus (HPV) infection is the main etiological factor for cervical intraepithelial lesions (CIN). An important characteristic of this process is the loss of genome stability. Therefore, it is imperative to use biomarkers of DNA damage caused by genomic instability to identify high risk individuals. We investigated the frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) of 20 patients, diagnosed as histologically CIN 1 and 10 healthy controls. We also examined the frequency of other nuclear anomalies including nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL of patients with CIN 1 and healthy controls, and evaluated the benefits of p16INK4a and Ki-67 (p16INK4a /Ki-67) immunohistochemical double staining for identifying cervical squamous cells that express HPV E6/E7 oncogenes...
May 23, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29728850/liquid-based-cytology-do-ancillary-techniques-enhance-detection-of-epithelial-abnormalities
#2
Sridhar Mishra, Nuzhat Husain, Namrata P Awasthi, Yashodhara Pradeep, Roohi Roohi, Sarita Saxena
PURPOSE: Cervical cancer is the fourth most common cancer in women worldwide with very high incidence in India. Liquid-based cytology (LBC) provides the use of ancillary techniques in addition to a good morphology and detection of cytologic abnormalities. The current study was designed to assess the diagnostics of P16INK4a immunoexpression, p16 promoter hypermethylation, human papilloma virus (HPV), and DNA ploidy in LBC samples with cervical precancer and cancer. METHODS: A series of LBC samples categorised by Bethesda system including 22 atypical squamous cells of undetermined significance (ASC-US), 21 low-grade squamous intraepithelial lesion (LSIL), 41 high-grade squamous intraepithelial lesion (HSIL), 54 squamous cell carcinoma (SCC), and 26 controls with normal cytology were included...
May 4, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29721063/photoacoustic-imaging-as-an-early-biomarker-of-radio-therapeutic-efficacy-in-head-and-neck-cancer
#3
Laurie J Rich, Austin Miller, Anurag K Singh, Mukund Seshadri
The negative impact of tumor hypoxia on radiotherapeutic efficacy is well recognized. However, an easy to use, reliable imaging method for assessment of tumor oxygenation in routine clinical practice remains elusive. Photoacoustic imaging (PAI) is a relatively new imaging technique that utilizes a combination of light and ultrasound (US) to enable functional imaging of tumor hemodynamic characteristics in vivo . Several clinical trials are currently evaluating the utility of PAI in cancer detection for breast, thyroid, and prostate cancer...
2018: Theranostics
https://www.readbyqxmd.com/read/29707519/molecular-signatures-of-high-grade-cervical-lesions
#4
Andreia M Porcari, Fernanda Negrão, Guilherme Lucas Tripodi, Denise Rocha Pitta, Elisabete Aparecida Campos, Douglas Munhoz Montis, Aline M A Martins, Marcos N Eberlin, Sophie F M Derchain
Cervical cancer is the fourth most common neoplasia in women and the infection with human papilloma virus (HPV) is its necessary cause. Screening methods, currently based on cytology and HPV DNA tests, display low specificity/sensitivity, reducing the efficacy of cervical cancer screening programs. Herein, molecular signatures of cervical cytologic specimens revealed by liquid chromatography-mass spectrometry (LC-MS), were tested in their ability to provide a metabolomic screening for cervical cancer. These molecules were tested whether they could clinically differentiate insignificant HPV infections from precancerous lesions...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29698769/prevalence-of-human-papilloma-virus-with-risk-of-cervical-cancer-among-south-indian-women-a-genotypic-study-with-meta-analysis-and-molecular-dynamics-of-hpv-e6-oncoprotein
#5
R S Akram Husain, R Rajakeerthana, Anoop Sreevalsan, P Prema Jayaprasad, Shiek S S J Ahmed, V Ramakrishnan
Cervical cancer (CC) is a major fatal health problem in women with high mortality worldwide. Human Papilloma Virus (HPV) is considered as one of the causative factors for CC. The HPV prevalence and their genotype distribution among women population are essential to evaluate the deteriorating impact of HPV. A cross-sectional study was performed involving 212 participants to identify the prevalence of high-risk HPV genotypes in south India using PCR and DNA Sequencing. The results obtained from cross-sectional study were used to conduct a meta-analysis of the previous published studies on HPV prevalence and genotype distribution across six geographical regions (North, Northeast, East, Central, West, and South) of India...
April 23, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29688091/molecular-detection-of-hhv1-5-aav-and-hpv-in-semen-specimens-and-their-impact-on-male-fertility
#6
Emad Behboudi, Talat Mokhtari-Azad, Jila Yavarian, Nastaran Ghavami, Seyed Mahmood Seyed Khorrami, Farhad Rezaei, Javad Charostad, Somayeh Shatizadeh Malekshahi, Nazanin Zahra Shafiei-Jandaghi
Viral infections have been considered as possible destructive factors that influence male fertility. The aim of this study was to determine the prevalence of human herpes viruses 1-5 (HHV1-5), adeno associated virus (AAV) and human papilloma virus (HPV) in semen and whether these influence semen quality. DNA extraction was performed using phenol-chloroform protocol, then three different nested-PCRs were done to detect HHV1-5, AAV and HPV DNAs in the semen samples. Of 145 samples, 66 (45.5%) were positive at least for one of the viruses...
April 24, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29652189/a-review-and-update-on-papillary-immature-metaplasia-of-the-uterine-cervix-a-distinct-subset-of-low-grade-squamous-intraepithelial-lesion-proposing-a-possible-cell-of-origin
#7
Soon Auck Hong, Su Hyun Yoo, Jene Choi, Stanley J Robboy, Kyu-Rae Kim
CONTEXT: - Papillary immature metaplasia (PIM) is a known papillary cervical lesion associated with low-risk human papilloma virus (LR-HPV). OBJECTIVE: - To evaluate additional clinicopathologic features and the HPV genotypes of PIM and discuss the presumptive cell of origin. DESIGN: - A total of 26 PIM cases were evaluated by p16INK4a , cytokeratin (CK) 7, and CK17 immunohistochemical stainings. Human papilloma virus genotyping was performed, by using HPV DNA Chip, HPV polymerase chain reaction (PCR), and real-time PCR...
April 13, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29590262/pcr-rflp-assay-as-an-option-for-primary-hpv-test
#8
L Golfetto, E V Alves, T R Martins, T C M Sincero, J B S Castro, C Dannebrock, J G Oliveira, J E Levi, A S C Onofre, M L Bazzo
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24...
March 26, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29576839/simultaneous-detection-of-multiple-hpv-dna-via-bottom-well-microfluidic-chip-within-an-infra-red-pcr-platform
#9
Wenjia Liu, Antony Warden, Jiahui Sun, Guangxia Shen, Xianting Ding
Portable Polymerase Chain Reaction (PCR) devices combined with microfluidic chips or lateral flow stripes have shown great potential in the field of point-of-need testing (PoNT) as they only require a small volume of patient sample and are capable of presenting results in a short time. However, the detection for multiple targets in this field leaves much to be desired. Herein, we introduce a novel PCR platform by integrating a bottom-well microfluidic chip with an infra-red (IR) excited temperature control method and fluorescence co-detection of three PCR products...
March 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29560516/combinational-chromosomal-aneuploidies-and-hpv-status-for-prediction-of-head-and-neck-squamous-cell-carcinoma-prognosis-in-biopsies-and-cytological-preparations
#10
Silke Wemmert, Maximilian Linxweiler, Cornelia Lerner, Florian Bochen, Philipp Kulas, Johannes Linxweiler, Sigrun Smola, Steffi Urbschat, Stefan Wagenpfeil, Bernhard Schick
PURPOSE: Head and neck squamous cell carcinoma (HNSCC) is one of the most common human cancer types with a very poor prognosis despite improvements in therapeutic modalities. The major known risk factors are tobacco use and alcohol consumption or infection with high-risk human papilloma viruses (HPV), especially in oropharyngeal tumors. The current management based on the assessment of a variety of clinical and pathological parameters does not sufficiently predict outcome. METHODS: Chromosomal alterations detected in HNSCCs were characterized by metaphase comparative genomic hybridization (CGH) and correlated with clinical parameters as well as survival time...
June 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29547549/hpv-virus-transcriptional-status-assessment-in-a-case-of-sinonasal-carcinoma
#11
Gennaro Ilardi, Daniela Russo, Silvia Varricchio, Giovanni Salzano, Giovanni Dell'Aversana Orabona, Virginia Napolitano, Rosa Maria Di Crescenzo, Alessandra Borzillo, Francesco Martino, Francesco Merolla, Massimo Mascolo, Stefania Staibano
Human Papilloma Virus (HPV) can play a causative role in the development of sinonasal tract malignancies. In fact, HPV may be the most significant causative agent implicated in sinonasal tumorigenesis and is implicated in as many as 21% of sinonasal carcinomas. To date, there are no definitive, reliable and cost-effective, diagnostic tests approved by the FDA for the unequivocal determination of HPV status in head and neck cancers. We followed an exhaustive algorithm to correctly test HPV infection, including a sequential approach with p16INK4a IHC, viral DNA genotyping and in situ hybridization for E6/E7 mRNA...
March 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29533280/polymorphous-sweat-gland-carcinoma-an-immunohistochemical-and-molecular-study
#12
Shira Ronen, Irene Aguilera-Barrantes, Tamara Giorgadze, Petr Šteiner, Petr Grossmann, Saul Suster
Polymorphous sweat gland carcinoma is an uncommon low-grade malignant adnexal tumor with a marked predilection for the distal extremities. Histologically, the lesions are characterized by a cellular proliferation showing a combination of growth patterns, including trabecular, solid, tubular, cribriform, or adenoid cystic and pseudopapillary. The immunohistochemical and molecular profile of these tumors has not yet been properly addressed. We have studied 3 cases of polymorphous sweat gland carcinoma using a broad panel of immunohistochemical markers including cytokeratin AE1/AE3, CK5/6, MOC31, p40, p63, p16, chromogranin, synaptophysin, CD56, MIB-1, estrogen receptor, progesterone receptor, androgen receptor, BER-EP4, smooth muscle actin, epithelial membrane antigen, carcinoembryonic antigen, CD117, S100 protein, HBME-1, DOG1, vimentin, and mammaglobin...
March 6, 2018: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29503262/the-implications-of-male-human-papilloma-virus-infection-in-couples-seeking-assisted-reproduction-technologies
#13
Gamze Sinem Çağlar, Nicolas Garrido
Human papilloma virus (HPV) is one of the most common viral sexually-transmitted diseases worldwide. The prevalence of HPV is higher in infertile males when compared with fertile men and ranges between 10 and 35.7% in men affected by unexplained infertility. HPV can bind to spermatozoa and can potentially be transferred to fertilized oocytes. Viral detection in blastocysts and trophoblastic cells is associated with impaired embryo development and poor pregnancy outcomes. Nevertheless, attempts to eliminate HPV-DNA from sperm samples through routine washing techniques have failed...
March 1, 2018: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/29502149/a-teat-papillomatosis-case-in-a-damascus-goat-shami-goat-in-hatay-province-turkey-a-new-putative-papillomavirus
#14
Fırat Dogan, Selvi Deniz Dorttas, Seval Bilge Dagalp, Veysel Soydal Ataseven, Feray Alkan
Papillomaviruses (PVs) are epitheliotropic viruses that cause benign proliferative lesions in the skin (warts or papillomas) and mucous membranes of their natural hosts. Recently, new PVs have been found in many animal species. The most common current approach for identifying novel PV types is based on PCR, using various consensus or degenerated primer (broad-range primers), designed on the basis of the multiple alignment of nucleotide or amino acid sequences of a large number of different human papillomaviruses (HPV)...
March 3, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29491584/high-risk-human-papillomavirus-in-the-periodontium-a-case-control-study
#15
Anna Shipilova, Manjunath Mundoor Dayakar, Dinesh Gupta
Background: Human papilloma viruses (HPVs) are small DNA viruses that have been identified in periodontal pocket as well as gingival sulcus. High risk HPVs are also associated with a subset of head and neck carcinomas. It is thought that the periodontium could be a reservoir for HPV. Aims: 1. Detection of Human Papilloma virus (HPV) in periodontal pocket as well as gingival of patients having localized chronic periodontitis and gingival sulcus of periodontally healthy subjects...
September 2017: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29478287/altered-epigenetic-pathways-and-cell-cycle-dysregulation-in-healthy-appearing-skin-of-patients-with-koebnerized-squamous-cell-carcinomas-following-skin-surgery
#16
T Gambichler, I Rüddel, S Hessam, F G Bechara, E Stockfleth, L Schmitz
BACKGROUND: Koebnerized non-melanoma skin cancer following skin trauma represents a rare and obscure event. OBJECTIVES: To study molecular pathological parameters in koebnerized squamous cell carcinomas (K-SCCs) occurring after complete tumour removal. METHODS: We assessed two patients with multiple sclerosis who were on treatment with dimethylfumarate (DMF) preceded by long-term azathioprine therapy. Both patients rapidly developed several K-SCCs following histopathologically proven complete excision of cutaneous SCCs...
February 25, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29460521/hpv-acting-on-e-cadherin-p53-and-p16-literature-review
#17
L Ottria, V Candotto, F Cura, L Baggi, C Arcuri, M Nardone, R M Gaudio, R Gatto, F Spadari, F Carinci
In addition to tobacco and alcohol consumption, the two main risk factors for oral squamous cell carcinoma (OSCC), recent studies have revealed infections with human papilloma virus (HPV) as an additional risk factor for OSCC development. In the field of head and neck malignancies, the prevalence of HPV infections in oropharyngeal cancer (OC) ranges in different studies up to 84%. While HPV infection is discussed as an independent risk factor in this region, its distinguished role in carcinogenesis of tumours localized to the oral cavity remains uncertain...
January 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29455435/dna-methylation-regulated-micrornas-in-hpv-16-induced-head-and-neck-squamous-cell-carcinoma-hnscc
#18
M K Sannigrahi, Rajni Sharma, Varinder Singh, Naresh K Panda, Vidya Rattan, Madhu Khullar
INTRODUCTION: Epigenetic modifications have been reported to play an important role in regulating gene expression and these modifications become critical when they have a role in controlling another important layer of epigenetic regulation namely microRNAs. In the present study, we have identified the microRNAs that may be regulated by promoter DNA methylation and histone acetylation in Human papilloma virus-positive head and neck squamous cell carcinoma. METHODOLOGY: HPV-negative cell line (UPCI:SCC-116) and HPV-16 +ve cell line (UPCI:SCC-090) were treated with methylation inhibitor (5-aza-2'-deoxycytidine, AZA) and acetylation inhibitor (Trichostatin-A, TSA), followed by micro-array analysis...
February 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29432879/human-papilloma-virus-16-18-fabricator-of-trouble-in-oral-squamous-cell-carcinoma
#19
Zil-E-Rubab, Saeeda Baig, Uzma Zaman, Mohammad Haris Lucky
AIM: To find out the association between Human Papilloma Virus (HPV) genotypes 16/18 in Pakistani patients with oral squamous cell carcinoma (OSCC). METHODS: DNA from oral rinse of 300 subjects was taken. The subjects included 100 cases with OSCC and 200 controls. Samples were analyzed by both conventional and real time PCR using "HPV consensus Gp5+/Gp6+ and HPV 16, 18 specific primers". RESULTS: Out of 300 persons, 74/300 (25%) were found to be infected with HPV: "46/100(46%) from cases and 74/200(14%) from controls"...
April 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29425237/intragenic-dna-methylation-of-pitx1-and-the-adjacent-long-non-coding-rna-c5orf66-as1-are-prognostic-biomarkers-in-patients-with-head-and-neck-squamous-cell-carcinomas
#20
Verena Sailer, Arthur Charpentier, Joern Dietrich, Timo J Vogt, Alina Franzen, Friedrich Bootz, Dimo Dietrich, Andreas Schroeck
BACKGROUND: Patients with squamous cell cancer of the head and neck region (HNSCC) are at risk for disease recurrence and metastases, even after initial successful therapy. A tissue-based biomarker could be beneficial to guide treatment as well as post-treatment surveillance. Gene methylation status has been recently identified as powerful prognostic biomarker in HNSCC. We therefore evaluated the methylation status of the homeobox gene PITX1 and the adjacent long intergenic non-coding RNA (lincRNA) C5orf66-AS1 in publicly available datasets...
2018: PloS One
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