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Functional clotting assay

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https://www.readbyqxmd.com/read/27926787/comparison-of-the-effects-of-a-balanced-crystalloid-based-and-a-saline-based-tetrastarch-solution-on-canine-whole-blood-coagulation-and-platelet-function
#1
Annina Reuteler, Shannon Axiak-Flammer, Judith Howard, Katja-Nicole Adamik
OBJECTIVE: To evaluate the effects of a 6% hydroxyethyl starch (130/0.42) solution in either a buffered, electrolyte-balanced (HES-BAL), or a saline (HES-SAL) carrier solution on canine platelet function and whole blood coagulation. DESIGN: Prospective, randomized study. SETTING: University teaching hospital. ANIMALS: Thirty-seven client-owned dogs undergoing general anesthesia for arthroscopy or imaging studies. INTERVENTIONS: Dogs received a 15 mL/kg intravenous bolus of HES-SAL (n = 13), HES-BAL (n = 14), or a modified Ringer's solution (n = 10) over 30-40 minutes...
December 7, 2016: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/27918018/a-planar-model-of-the-vessel-wall-from-cellularized-collagen-scaffolds-focus-on-cell-matrix-interactions-in-mono-bi-and-tri-culture-models
#2
Caroline Loy, Sébastien Meghezi, Lucie Lévesque, Daniele Pezzoli, Heena Kumra, Dieter Reinhardt, Jayachandran N Kizhakkedathu, Diego Mantovani
The acquisition of new thorough knowledge on the interactions existing between vascular cells would represent a step forward in the engineering of vascular tissues. In this light, herein we designed a physiological-like tri-culture in vitro vascular wall model using a planar cellularized collagen gel as the scaffold. The model can be obtained in 24 h and features multi-layered hierarchical organization composed of a fibroblast-containing adventitia-like layer, a media-like layer populated by smooth muscle cells and an intima-like endothelial cell monolayer...
December 5, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27917409/gadolinium-functionalized-peptide-amphiphile-micelles-for-multimodal-imaging-of-atherosclerotic-lesions
#3
Sang Pil Yoo, Federico Pineda, John C Barrett, Christopher Poon, Matthew Tirrell, Eun Ji Chung
The leading causes of morbidity and mortality globally are cardiovascular diseases, and nanomedicine can provide many improvements including disease-specific targeting, early detection, and local delivery of diagnostic agents. To this end, we designed fibrin-binding, peptide amphiphile micelles (PAMs), achieved by incorporating the targeting peptide cysteine-arginine-glutamic acid-lysine-alanine (CREKA), with two types of amphiphilic molecules containing the gadoliniuim (Gd) chelator diethylenetriaminepentaacetic acid (DTPA), DTPA-bis(stearylamide)(Gd), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[(poly(ethylene glycol) (PEG))-2000]-DTPA(Gd) (DSPE-PEG2000-DTPA(Gd))...
November 30, 2016: ACS Omega
https://www.readbyqxmd.com/read/27901294/performance-of-functional-fibrinogen-thromboelastography-in-children-undergoing-congenital-heart-surgery
#4
Nischal K Gautam, Chunyan Cai, Olga Pawelek, Muhammad B Rafique, Davide Cattano, Evan G Pivalizza
BACKGROUND: Functional Fibrinogen assay of the Thromboelastography (FFTEG), a whole blood viscoelastic hemostatic assay, has been used to estimate fibrinogen levels in adult patients undergoing major surgery but its performance in pediatric patients undergoing cardiac surgery requires evaluation. In this study, we evaluate the correlation between FFTEG parameters and standard laboratory tests for fibrinogen and platelet counts before and after cardiopulmonary bypass in children undergoing repair for congenital heart disease...
November 30, 2016: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/27892731/is-arachidonic-acid-stimulation-really-a-test-for-the-response-to-aspirin-time-to-think-again
#5
Bartosz Olechowski, Alexander Ashby, Mark Mariathas, Vikram Khanna, Michael Mahmoudi, Nick Curzen
Platelets play a key role in pathogenesis of atherothrombosis. Activated platelets initiate thrombus formation. Antiplatelet therapy (APT) modifies these properties. APT involves aspirin. The existence of "aspirin resistance" is reported in many populations with cardiovascular disease. The prevalence of this phenomenon is highly variable, affecting more than 50% of patient subgroups in some papers. Areas covered: This review describes the prevalence of "aspirin resistance", analyses why there is so much apparent variation and addresses whether the commonly used tests of aspirin response are in fact accurately assessing its functional performance...
November 28, 2016: Expert Review of Cardiovascular Therapy
https://www.readbyqxmd.com/read/27874025/beyond-antibodies-development-of-a-novel-protein-scaffold-based-on-human-chaperonin-10
#6
Abdulkarim M Alsultan, David Y Chin, Christopher B Howard, Christopher J de Bakker, Martina L Jones, Stephen M Mahler
Human Chaperonin 10 (hCpn10) was utilised as a novel scaffold for presenting peptides of therapeutic and diagnostic significance. Molecular dynamic simulations and protein sizing analyses identified a peptide linker (P1) optimal for the formation of the quarternary hCpn10 heptamer structure. hCpn10 scaffold displaying peptides targeting Factor VIIa (CE76-P1) and CD44 (CP7) were expressed in E. coli. Functional studies of CE76-P1 indicated nanomolar affinity for Factor VIIa (3 nM) similar to the E-76 peptide (6 nM), with undetectable binding to Factor X...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27845251/heparan-sulfate-heparin-glycosaminoglycan-binding-alters-inhibitory-profile-and-enhances-anticoagulant-function-of-conserved-amblyomma-americanum-tick-saliva-serpin-19
#7
Željko M Radulović, Albert Mulenga
Some serine protease inhibitor (serpin) regulators of essential life pathways bind glycosaminoglycans (GAGs) to enhance inhibitory functions and achieve physiologically relevant rates. This study demonstrates that highly conserved Amblyomma americanum tick saliva serpin 19 (AAS19), a broad-spectrum inhibitor of hemostasis and inflammation system proteases and anticoagulant, can bind heparan sulfate/heparin (HS)GAGs and that this interaction alters its function. Substrate hydrolysis and unpaired t-test analyses revealed that HSGAG binding caused rAAS19 inhibitory activity to: (i) significantly increase against blood clotting factors (f) IIa (thrombin) and fIXa, (ii) significantly reduce against fXa and fXIIa and (iii) moderate to no effect against trypsin, kallikrein, papain, and plasmin...
November 12, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27779583/model-of-trauma-induced-coagulopathy-including-hemodilution-fibrinolysis-acidosis-and-hypothermia-impact-on-blood-coagulation-and-platelet-function
#8
Boris Shenkman, Ivan Budnik, Yulia Einav, Hagit Hauschner, Mykhaylo Andrejchin, Uriel Martinowitz
BACKGROUND: Trauma-induced coagulopathy (TIC) is commonly seen among patients with severe injury. The dynamic process of TIC is characterized by variability of the features of the disease. METHODS: A model of TIC was created. Hemodilution was produced by mixing the blood with 40% TRIS/saline solution, fibrinolysis by treating the blood with 160 ng/mL tPA, acidosis by adding 1.2 mg/mL lactic acid achieving pH 7.0-7.1, and hypothermia by running the assay at 31 °C...
October 25, 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27778524/the-actin-binding-proteins-cortactin-and-hs1-are-dispensable-for-platelet-actin-nodule-and-megakaryocyte-podosome-formation
#9
Steven G Thomas, Natalie S Poulter, Danai Bem, Brenda Finney, Laura M Machesky, Stephen P Watson
A dynamic, properly organised actin cytoskeleton is critical for the production and haemostatic function of platelets. The Wiskott Aldrich Syndrome protein (WASp) and Actin-Related Proteins 2 & 3 Complex (Arp2/3 complex) are critical mediators of actin polymerisation and organisation in many cell types. In platelets and megakaryocytes, these proteins have been shown to be important for proper platelet production and function. The cortactin family of proteins (Cttn & HS1) are known to regulate WASp-Arp2/3-mediated actin polymerisation in other cell types and so here we address the role of these proteins in platelets using knockout mouse models...
October 25, 2016: Platelets
https://www.readbyqxmd.com/read/27747653/in-vitro-immunological-and-biological-evaluations-of-the-angiogenic-potential-of-platelet-rich-fibrin-preparations-a-standardized-comparison-with-prp-preparations
#10
Mito Kobayashi, Tomoyuki Kawase, Kazuhiro Okuda, Larry F Wolff, Hiromasa Yoshie
BACKGROUND: Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios...
December 2015: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/27711024/reference-intervals-of-thromboelastometric-evaluation-of-coagulation-in-pediatric-patients-with-congenital-heart-diseases-a-retrospective-investigation
#11
Ji Young Kim, Yu Rim Shin, Hae Keum Kil, Mi Ran Park, Jong Wha Lee
BACKGROUND Rotational thromboelastometry (ROTEM®) is a point-of-care test for coagulation, enabling physicians to make a swift decision. The aim of this investigation was to establish reference intervals of thromboelastometric evaluation for coagulation in pediatric patients with congenital heart diseases (CHD). MATERIAL AND METHODS As baseline data, 3 assays of ROTEM® (INTEM, EXTEM, and FIBTEM) were measured after anesthesia induction. ROTEM® parameters were clotting time (CT), amplitude at 10 min (A10), clot formation time (CFT), a angle, maximal clot firmness (MCF), clot lysis index at 60 min (LI60), and maximal clot elasticity (MCE)...
October 6, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27686864/the-impact-of-p2y12-promoter-dna-methylation-on-the-recurrence-of-ischemic-events-in-chinese-patients-with-ischemic-cerebrovascular-disease
#12
Xin-Gang Li, Ning Ma, Bo Wang, Xiao-Qing Li, Sheng-Hui Mei, Kun Zhao, Yong-Jun Wang, Wei Li, Zhi-Gang Zhao, Shu-Sen Sun, Zhong-Rong Miao
The primary mechanism of clopidogrel resistance is still unclear. We aimed to investigate whether the methylation status of the P2Y12 promoter has effects on platelet function and clinical ischemic events. Patients with ischemic cerebrovascular disease were enrolled into our study. Venous blood samples were drawn for thrombelastograpy (TEG) and active metabolite assay. Patients were divided into a case- or control-group based on the occurrence of ischemic events during a one year follow-up. Two TEG parameters between the case and control groups were statistically significant [ADP inhibition rate (ADP%): P = 0...
September 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27684817/three-cases-of-congenital-dysfibrinogenemia-in-unrelated-chinese-families-heterozygous-missense-mutation-in-fibrinogen-alpha-chain-argl6his
#13
Meiling Luo, Donghong Deng, Liqun Xiang, Peng Cheng, Lin Liao, Xuelian Deng, Jie Yan, Faquan Lin
Congenital dysfibrinogenemia (CD) is a qualitative fibrinogen disorder caused by an abnormal fibrinogen molecule structure, leading to dysfunctional blood coagulation. This study describes 3 cases of dysfibrinogenemia identified in the unrelated Chinese pedigrees.Routine coagulation screening tests were performed on the probands and their families. The antigens and functionality of fibrinogen was measured using an immunoturbidimetry assay and the Clauss method, respectively. To identify the genetic mutation responsible for these dysfibrinogens, genomic DNA extracted from the blood was analyzed using PCR amplification and direct sequencing...
September 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27607599/temporal-changes-in-clot-lysis-and-clot-stability-following-tranexamic-acid-in-cardiac-surgery
#14
Mariann Tang, Per Wierup, Catherine J Rea, Jrgen Ingerslev, Vibeke E Hjortdal, Benny Srensen
Cardiac surgery induces a multifactorial coagulopathy. Regular use of tranexamic acid (TXA) is becoming standard of care. Clinical challenges include selecting optimal dosing regimen and balancing the benefit versus risk of additional dosing with antifibrinolytics. The objective was to evaluate the effect of TXA by assessing kinetic properties of plasma clot formation, clot stability, and clot fibrinolysis. The study was a prospective case follow-up of 28 patients undergoing cardiac surgery (mean age 63.9 years, 29% women)...
September 2, 2016: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/27604413/generation-and-in-vitro-characterisation-of-inhibitory-nanobodies-towards-plasminogen-activator-inhibitor-1
#15
Xiaohua Zhou, Maarten L V Hendrickx, Gholamreza Hassanzadeh-Ghassabeh, Serge Muyldermans, Paul J Declerck
Plasminogen activator inhibitor 1 (PAI-1) is the principal physiological inhibitor of tissue-type plasminogen activator (t-PA) and has been identified as a risk factor in cardiovascular diseases. In order to generate nanobodies against PAI-1 to interfere with its functional properties, we constructed three nanobody libraries upon immunisation of three alpacas with three different PAI-1 variants. Three panels of nanobodies were selected against these PAI-1 variants. Evaluation of the amino acid sequence identity of the complementarity determining region-3 (CDR3) reveals 34 clusters in total...
November 30, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27604259/missense-mutations-in-the-gene-encoding-prothrombin-corresponding-to-arg596-cause-antithrombin-resistance-and-thrombomodulin-resistance
#16
Yuki Takagi, Moe Murata, Toshihiro Kozuka, Yukiko Nakata, Ryo Hasebe, Shogo Tamura, Akira Takagi, Tadashi Matsushita, Hidehiko Saito, Tetsuhito Kojima
Antithrombin (AT) and thrombomodulin (TM) play important roles in the process of natural anticoagulation in vivo. Recently, we reported that the prothrombin Yukuhashi mutation (p.Arg596Leu) was associated with AT and TM resistance-related thrombophilia. To assess the AT and TM resistances associated with other missense mutations by single base substitution in the Arg596 codon, we generated recombinant variants (596Gln, 596Trp, 596Gly, and 596Pro) and investigated the effects on AT and TM anticoagulant functions...
November 30, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27585844/a-common-suite-of-coagulation-proteins-function-in-drosophila-muscle-attachment
#17
Nicole Green, Nadia Odell, Molly Zych, Cheryl Clark, Zong-Heng Wang, Bridget Biersmith, Clara Bajzek, Kevin R Cook, Mitchell S Dushay, Erika Geisbrecht
The organization and stability of higher order structures that form in the extracellular matrix (ECM) to mediate the attachment of muscles are poorly understood. We have made the surprising discovery that a subset of clotting factor proteins are also essential for muscle attachment in the model organism Drosophila melanogaster One such coagulation protein, Fondue (Fon), was identified as a novel muscle mutant in a pupal lethal genetic screen. Fon accumulates at muscle attachment sites and removal of this protein results in decreased locomotor behavior and detached larval muscles...
August 31, 2016: Genetics
https://www.readbyqxmd.com/read/27584686/seer-sonorheometry-versus-rotational-thromboelastometry-in-large-volume-blood-loss-spine-surgery
#18
Bhiken I Naik, Marcel E Durieux, Anne Knisely, Juhee Sharma, Vivien C Bui-Huynh, Bhavana Yalamuru, Abdullah S Terkawi, Edward C Nemergut
BACKGROUND: Sonic estimation of elasticity via resonance (SEER) sonorheometry is a novel technology that uses acoustic deformation of the developing clot to measure its viscoelastic properties and extract functional measures of coagulation. Multilevel spine surgery is associated with significant perioperative blood loss, and coagulopathy occurs frequently. The aim of this study was to correlate SEER sonorheometry results with those of equivalent rotation thromboelastometry (ROTEM) and laboratory parameters obtained during deformity correction spine surgery...
December 2016: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/27555433/dysfibrinogenemia-associated-novel-heterozygous-mutation-shanghai-fga-c-169_180-2-del-leads-to-n-terminal-truncation-of-fibrinogen-a%C3%AE-chain-and-impairs-fibrin-polymerization
#19
Jingyi Zhou, Qiulan Ding, Wenman Wu, Qi Ouyang, Yinyin Xie, Xi Wu, Yeling Lu, Jing Dai, Qian Liang, Hongli Wang, Xuefeng Wang, Yiqun Hu
AIMS: A novel heterozygous variant, FGA c.169_180+2 del (designated fibrinogen Shanghai), was identified in a patient with dysfibrinogenemia with antiphospholipid antibody syndrome (APS) and recurrent venous thrombosis, and in his asymptomatic father. We aimed to reveal the functional implication of structural change caused by this variant. METHODS: Transcription analysis was performed with FGA minigene transfection assay to evaluate the impact of nucleosides deletion on mRNA editing...
August 23, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27541720/comparison-of-thrombelastography-derived-fibrinogen-values-at-rewarming-and-following-cardiopulmonary-bypass-in-cardiac-surgery-patients
#20
Michael Fabbro, Jacob T Gutsche, Todd A Miano, John G Augoustides, Prakash A Patel
BACKGROUND: The inflated costs and documented deleterious effects of excess perioperative transfusion have led to the investigation of targeted coagulation factor replacement strategies. One particular coagulation factor of interest is factor I (fibrinogen). Hypofibrinogenemia is typically tested for using time-consuming standard laboratory assays. The thrombelastography (TEG)-based functional fibrinogen level (FLEV) provides an assessment of whole blood clot under platelet inhibition to report calculated fibrinogen levels in significantly less time...
September 2016: Anesthesia and Analgesia
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