keyword
MENU ▼
Read by QxMD icon Read
search

Functional clotting assay

keyword
https://www.readbyqxmd.com/read/29675848/the-role-of-barrels-1-and-2-in-the-enzymatic-activity-of-factor-xiii-a
#1
E L Hethershaw, Penelope J Adamson, Kerrie A Smith, Whitney N Goldsberry, Richard J Pease, Sheena E Radford, Peter J Grant, Robert A S Ariëns, Muriel C Maurer, Helen Philippou
BACKGROUND: Factor XIII (FXIII) is composed of an activation peptide segment, a β-sandwich domain, a catalytic core, and finally β-barrels 1 and 2. FXIII is activated following cleavage of its A-subunits by thrombin. The resultant transglutaminase activity leads to increased resistance of fibrin clots to fibrinolysis. OBJECTIVES: To assess the functional roles of β-barrels 1 and 2 in FXIII, we expressed and characterised the full-length FXIII-A subunit (FXIII-A) and variants truncated to residue 628 [truncated to β-barrel 1 (TB1)], 515 [truncated to catalytic core (TCC)] and 184 [truncated to β-sandwich (TBS)]...
April 19, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29652675/an-intronic-mutation-c-6430-3c-g-in-the-f8-gene-causes-splicing-efficiency-and-premature-termination-in-hemophilia-a
#2
Zunjing Xia, Jie Lin, Lingping Lu, Chol Kim, Ping Yu, Ming Qi
: Hemophilia A is a bleeding disorder caused by coagulation factor VIII protein deficiency or dysfunction, which is classified into severe, moderate, and mild according to factor clotting activity. An overwhelming majority of missense and nonsense mutations occur in exons of F8 gene, whereas mutations in introns can also be pathogenic. This study aimed to investigate the effect of an intronic mutation, c.6430-3C>G (IVS22-3C>G), on pre-mRNA splicing of the F8 gene. We applied DNA and cDNA sequencing in a Chinese boy with hemophilia A to search if any pathogenic mutation in the F8 gene...
April 12, 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/29626642/haemostatic-effects-of-latex-from-croton-sparsiflorus-morang-in-vitro-in-vivo-in-silico-approaches
#3
M C Kamaraj, S Mohan Raj, D Palani Selvam, S Subashchandrabose, A Kalaiselvan
The present investigations are phytochemical screening of Latex aqueous (Laq ) extract of C. sparsiflorus and study its role in homeostasis. It is being traditionally used for fresh cuts to stop bleeding immediately. To know the contents of extract, the quantitative phytochemical analysis were performed it showed the contents such as saponins (15.2%), alkaloids (7.61%), phenols (0.62%), tannins (1.1%), and flavonoids (0.224%). The in vitro and in vivo blood clotting mechanism was observed in Wister albino rats to understand the blood clotting activity...
March 27, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29615672/morphometric-analysis-of-spread-platelets-identifies-integrin-%C3%AE-iib-%C3%AE-3-specific-contractile-phenotype
#4
Sebastian Lickert, Simona Sorrentino, Jan-Dirk Studt, Ohad Medalia, Viola Vogel, Ingmar Schoen
Haemostatic platelet function is intimately linked to cellular mechanics and cytoskeletal morphology. How cytoskeletal reorganizations give rise to a highly contractile phenotype that is necessary for clot contraction remains poorly understood. To elucidate this process in vitro, we developed a morphometric screen to quantify the spatial organization of actin fibres and vinculin adhesion sites in single spread platelets. Platelets from healthy donors predominantly adopted a bipolar morphology on fibrinogen and fibronectin, whereas distinguishable, more isotropic phenotypes on collagen type I or laminin...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29603238/cold-storage-of-platelets-in-platelet-additive-solution-an-in-vitro-comparison-of-two-food-and-drug-administration-approved-collection-and-storage-systems
#5
Kristin M Reddoch-Cardenas, Robbie K Montgomery, Crystal B Lafleur, Grantham C Peltier, James A Bynum, Andrew P Cap
BACKGROUND: Refrigeration of platelets (PLTs) in a PLT additive solution (PAS) reduces PLT activation compared to storage in plasma and preserves function for at least 15 days. Currently only two PASs are licensed by the Food and Drug Administration, each for use with only one apheresis platform. In this study, we compared the metabolic, functional, and activation status of PLTs collected on a Trima apheresis collection system and stored refrigerated in Isoplate (ISO) PAS to PLTs collected on an Amicus collection system and stored refrigerated in Intersol (INT) PAS...
March 30, 2018: Transfusion
https://www.readbyqxmd.com/read/29579244/dna-damage-response-of-4-chloro-ortho-toluidine-in-various-rat-tissues
#6
Melanie Guerard, Christine Marchand, Jürgen Funk, Francois Christen, Michael Winter, Andreas Zeller
The potential of 4-chloro-ortho-toluidine (4-CloT), an aromatic amine substituted on the ortho- and para-position of the amine function, to induce DNA damage in male Wistar rats was evaluated with the micronucleus test (peripheral blood), Pig-a (peripheral blood) and comet assay (peripheral blood, liver, urinary bladder, jejunum) at several time points. In addition to those markers of DNA damage, i.e. gene mutation and clastogenicity, standard hematology, including methemoglobin (MetHb), histopathology and immunohistochemistry of γ-H2AX and Ki-67 in liver, jejunum and urinary bladder were performed...
March 20, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29575615/spectrum-of-hemostatic-disorders-in-indian-females-presenting-with-bleeding-manifestations
#7
A Gupta, P Mishra, H P Pati, S Tyagi, M Mahapatra, T Seth, R Saxena
INTRODUCTION: Hemostatic disorders are often missed in women with bleeding particularly menorrhagia. Preexisting hemostatic disorders are now known as common risk factor for postpartum hemorrhage and prolonged bleeding in puerperium. Females with bleeding complaints constitute an important population referred to hematology clinic. Hence, we aim to evaluate the type and frequency of hemostatic disorders among females presenting with bleeding in a tertiary care hospital and a basic hemostatic laboratory...
March 25, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29573326/platelets-loaded-with-thrombin-encapsulated-liposomes-have-increased-coagulability
#8
Vivienne Chan, Morteza Sarkari, Rachel Sunderland, Alexander E St John, Nathan J White, Christian J Kastrup
BACKGROUND: Platelets are integral to clot formation, and are often transfused to stop or prevent bleeding. However, transfusions of platelets are not always effective, particularly in the most severe cases of hemorrhage. Nanoparticle systems have been developed to mimic platelets but inherently lack important aspects of platelet function which limits their potential effectiveness. OBJECTIVES: Increasing the natural coagulability of transfusable platelets could increase their efficacy during treatment of severe hemorrhage...
March 24, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29541637/establishment-of-the-world-health-organization-first-international-standard-for-factor-xii-plasma-human
#9
Helen V Wilmot, Jason Hockley, Peter Rigsby, Elaine Gray
Until recently, the role of factor XII (FXII) in hemostasis was not considered to be important since patients with FXII deficiency do not present with bleeding. The activation of FXII by agents including mast cells and platelet polyphosphates suggests that it may have a role in thrombogenesis. The inhibition of FXII therefore presents an option for antithrombotic therapy, and antibodies and inhibitors are already in development. Assays for FXII will be required to support these technologies, and an international standard (IS) for FXII would be useful for the development of these methods and for the clinical monitoring of patients...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29538003/a-novel-mutation-tyr503cys-in-a-severe-factor-xi-deficiency
#10
Kankan Su, Xiaoxiao Cai, Wenli Xia, Yanhui Jin, Lihong Yang, Mingshan Wang
: The objective is to study a gene mutation (Tyr503Cys) found in a Chinese consanguineous marriage family with inherited factor XI (FXI) deficiency (cross-reacting material positive, type II). The FXI activity and FXI antigen were tested with clotting assay and ELISA, respectively. The FXI gene was amplified by PCR with direct sequencing. ClustalX-2.1-win and three online bioinformatics softwares were used to study the conservatism and harm of the mutation. The proband had reduced FXI: activity at 13%; three members had decreased to about 35%, all of whom had nomal FXI: antigen...
March 13, 2018: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/29525568/a-new-assay-for-global-fibrinolysis-capacity-gfc-investigating-a-critical-system-regulating-hemostasis-and-thrombosis-and-other-extravascular-functions
#11
REVIEW
Jean Amiral, Maxime Laroche, Jerard Seghatchian
For many years, the importance of fibrinolysis has been recognized, first for its intravascular antithrombotic action, and more recently for its many extravascular activities, associated with matrix degradation and tissue remodeling. In the blood circulation system, fibrinolysis prevents thrombosis, and is associated with various biological and clinical situations: risk factors for cardio-vascular diseases in high risk clinical situations (type II diabetes, hypertension, triglycerides, high BMI, elevated glucose, etc...
February 20, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29462691/factor-x-activating-atractaspis-snake-venoms-and-the-relative-coagulotoxicity-neutralising-efficacy-of-african-antivenoms
#12
Brice Oulion, James S Dobson, Christina N Zdenek, Kevin Arbuckle, Callum Lister, Francisco C P Coimbra, Bianca Op den Brouw, Jordan Debono, Aymeric Rogalski, Aude Violette, Rudy Fourmy, Nathaniel Frank, Bryan G Fry
Atractaspis snake species are enigmatic in their natural history, and venom effects are correspondingly poorly described. Clinical reports are scarce but bites have been described as causing severe hypertension, profound local tissue damage leading to amputation, and deaths are on record. Clinical descriptions have largely concentrated upon tissue effects, and research efforts have focused upon the blood-pressure affecting sarafotoxins. However, coagulation disturbances suggestive of procoagulant functions have been reported in some clinical cases, yet this aspect has been uninvestigated...
May 15, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29446525/laboratory-testing-for-factor-viii-and-ix-inhibitors-in-haemophilia-a-review
#13
REVIEW
C H Miller
Inhibitors are antibodies directed against haemophilia treatment products which interfere with their function. Factor VIII (FVIII) inhibitors in haemophilia A and factor IX (FIX) inhibitors in haemophilia B are significant clinically when they require a change in a patient's treatment regimen. Their persistence may increase morbidity and mortality. Multiple laboratory tests are now available for detecting and understanding inhibitors in haemophilia. Inhibitors are traditionally measured by their interference in clotting or chromogenic factor assays...
March 2018: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/29445039/both-lipopolysaccharide-and-lipoteichoic-acids-potently-induce-anomalous-fibrin-amyloid-formation-assessment-with-novel-amytracker%C3%A2-stains
#14
Etheresia Pretorius, Martin J Page, Lisa Hendricks, Nondumiso B Nkosi, Sven R Benson, Douglas B Kell
In recent work, we discovered that the presence of highly substoichiometric amounts (10 -8 molar ratio) of lipopolysaccharide (LPS) from Gram-negative bacteria caused fibrinogen clotting to lead to the formation of an amyloid form of fibrin. We here show that the broadly equivalent lipoteichoic acids (LTAs) from two species of Gram-positive bacteria have similarly (if not more) potent effects. Using thioflavin T fluorescence to detect amyloid as before, the addition of low concentrations of free ferric ion is found to have similar effects...
February 2018: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29442531/the-impact-of-pneumatic-tube-transport-on-whole-blood-coagulation-and-platelet-function-assays
#15
Peter H Nissen, Dorte E Wulff, Niels Tørring, Anne-Mette Hvas
Pneumatic tube is an attractive way to transport blood samples from the emergency department to the central laboratory facility. We aimed to investigate the impact of pneumatic tube transportation on blood samples for analysis of whole blood coagulation and platelet function. We included 21 healthy adult individuals and measured global coagulation assays by rotational thromboelastometry (ROTEM) and platelet aggregation induced by arachidonic acid (AA) and adenosine diphosphate (ADP) using impedance aggregometry (ROTEM Platelet), on samples transported manually or by pneumatic tube transport...
February 14, 2018: Platelets
https://www.readbyqxmd.com/read/29379088/the-inflammatory-effects-of-tnf-%C3%AE-and-complement-component-3-on-coagulation
#16
Martin J Page, Janette Bester, Etheresia Pretorius
Tissue necrosis factor-α (TNF-α) and complement component 3 (C3) are two well-known pro-inflammatory molecules. When TNF-α is upregulated, it contributes to changes in coagulation and causes C3 induction. They both interact with receptors on platelets and erythrocytes (RBCs). Here, we look at the individual effects of C3 and TNF-α, by adding low levels of the molecules to whole blood and platelet poor plasma. We used thromboelastography, wide-field microscopy and scanning electron microscopy to study blood clot formation, as well as structural changes to RBCs and platelets...
January 29, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29331940/screening-and-functional-exploration-of-prothrombin-arg596-related-mutations-in-chinese-venous-thromboembolism-patients
#17
Xi Wu, Lei Li, Qiulan Ding, Xuefeng Wang, Fang Wu, Wenman Wu
AIMS: Dysfunctional prothrombin residue Arg596 associated mutation has been found to precipitate venous thromboembolism (VTE). In the current study we investigated the prevalence of Arg596 associated mutations in Chinese patients with VTE and explored the functional impact of Arg596Gln mutation on coagulation function in affected patients. METHODS: Prothrombin clotting activity was measured in 267 unrelated patients with unprovoked VTE. Patients with moderately decreased activities underwent further analysis of the F2 gene...
January 13, 2018: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29304526/thromboelastometry-and-platelet-function-during-acclimatization-to-high-altitude
#18
Alistair S Rocke, Gordon G Paterson, Matthew T Barber, Alexander I R Jackson, Shona Main, Calum Stannett, Martin F Schnopp, J Kenneth Baillie, Elizabeth H Horne, Carl Moores, Paul Harrison, Alastair F Nimmo, A A Roger Thompson
Interaction between hypoxia and coagulation is important given the increased risk of thrombotic diseases in chronically hypoxic patients who reside at sea level and in residents at high altitude. Hypoxia alters the proteome of platelets favouring a prothrombotic phenotype, but studies of activation and consumption of specific coagulation factors in hypoxic humans have yielded conflicting results. We tested blood from 63 healthy lowland volunteers acclimatizing to high altitude (5,200 m) using thromboelastometry and assays of platelet function to examine the effects of hypoxia on haemostasis...
January 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29248181/principal-component-analysis-of-coagulation-assays-in-severely-injured-children
#19
Christine M Leeper, Matthew D Neal, Christine McKenna, Timothy Billiar, Barbara A Gaines
BACKGROUND: Trauma-induced coagulopathy is common and associated with poor outcome in injured children. Our aim is to identify patterns of coagulation dysregulation after injury and associate these phenotypes with relevant clinical outcomes. METHODS: We performed principal components analysis on prospectively collected data from children with the highest-level trauma activation June 2015-June 2016. Parameters included admission international normalized ratio, platelet count and thromboelastograms...
April 2018: Surgery
https://www.readbyqxmd.com/read/29239953/the-impact-of-prehospital-tranexamic-acid-on-blood-coagulation-in-trauma-patients
#20
Philipp Stein, Jan-Dirk Studt, Roland Albrecht, Stefan Müller, Dieter von Ow, Simon Fischer, Burkhardt Seifert, Sergio Mariotti, Donat R Spahn, Oliver M Theusinger
BACKGROUND: There is limited data on prehospital administration of tranexamic acid (TXA) in civilian trauma. The aim of this study was to evaluate changes in coagulation after severe trauma from on-scene to the hospital after TXA application in comparison to a previous study without TXA. METHODS: The study protocol was registered at ClinicalTrials.gov (NCT02354885). A prospective, multicenter, observational study investigating coagulation status in 70 trauma patients receiving TXA (1 g intravenously) on-scene versus a control group of 38 patients previously published without TXA...
February 2018: Anesthesia and Analgesia
keyword
keyword
11073
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"