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Tissue Preparation techniques

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https://www.readbyqxmd.com/read/28198427/in-vitro-and-in-vivo-responses-of-macrophages-to-magnesium-doped-titanium
#1
Bin Li, Huiliang Cao, Yaochao Zhao, Mengqi Cheng, Hui Qin, Tao Cheng, Yan Hu, Xianlong Zhang, Xuanyong Liu
Modulating immune response to biomaterials through changing macrophage polarization has been proven to be a promising strategy to elicit beneficial outcomes in tissue repair. The objective of this study was to evaluate the response of macrophage polarization to titanium doped with magnesium (0.1~0.35%), which was prepared through the magnesium plasma immersion ion implantation (Mg PIII) technique. The M1/M2 polarization profile of macrophages was investigated using a murine cell line RAW 264.7 in vitro and a murine air pouch model in vivo...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197025/current-perioperative-management-of-pheochromocytomas
#2
REVIEW
Rashmi Ramachandran, Vimi Rewari
Neuroendocrine tumors which have the potential to secrete catecholamines are either associated with sympathetic adrenal (pheochromocytoma) or nonadrenal (paraganglioma) tissue. Surgical removal of these tumors is always indicated to cure and prevent cardiovascular and other organ system complications associated with catecholamine excess. Some of these tumors have malignant potential as well. The diagnosis, localization and anatomical delineation of these tumors involve measurement of catecholamines and their metabolic end products in plasma and urine, (123)I-metaiodobenzylguanidine scintigraphy, computed tomography, and/or magnetic resonance imaging...
January 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28195392/relative-protein-quantification-and-accessible-biology-in-lung-tumor-proteomes-from-four-lc-ms-ms-discovery-platforms
#3
Paul A Stewart, Bin Fang, Robbert J C Slebos, Guolin Zhang, Adam L Borne, Katherine Fellows, Jamie K Teer, Y Ann Chen, Eric Welsh, Steven A Eschrich, Eric B Haura, John M Koomen
Discovery proteomics experiments include many options for sample preparation and mass spectrometry data acquisition, which are capable of creating datasets for quantifying thousands of proteins. To define a strategy that would produce a dataset with sufficient content while optimizing required resources, we compared 1) single sample liquid chromatography-tandem mass spectrometry (LC-MS/MS) with data dependent acquisition (DDA) to single sample LC-MS/MS with data independent acquisition (DIA) and 2) peptide fractionation with label-free quantification (LF) to isobaric labeling followed by peptide fractionation (TMT 6-plex)...
February 14, 2017: Proteomics
https://www.readbyqxmd.com/read/28193007/enhanced-spatially-resolved-proteomics-using-on-tissue-hydrogel-mediated-protein-digestion
#4
David G Rizzo, Boone M Prentice, Jessica L Moore, Jeremy L Norris, Richard M Caprioli
The identification of proteins from tissue specimens is a challenging area of biological research. Many current techniques for identification forfeit some level of spatial information during the sample preparation process. Recently, hydrogel technologies have been developed that perform spatially localized protein extraction and digestion prior to downstream proteomic analysis. Regiospecific protein identifications acquired using this approach have thus far been limited to 1-2 mm diameter areas. The need to target smaller populations of cells with this technology necessitates the production of smaller diameter hydrogels...
February 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28186651/rapid-immunocytochemistry-for-the-detection-of-cytokeratin-and-vimentin-assessment-of-its-diagnostic-value-in-neoplastic-diseases-of-dogs
#5
Mariko Sawa, Mayuko Inoue, Akira Yabuki, Moeko Kohyama, Noriaki Miyoshi, Asuka Setoguchi, Osamu Yamato
BACKGROUND: Immunocytochemistry (ICC) is an advanced diagnostic technique used in the field of veterinary cytology. We recently developed a rapid ICC method for the detection of cytokeratin and vimentin in dogs, which helps to determine whether tumor cells are of epithelial or nonepithelial origin. However, the diagnostic value of this rapid ICC method in neoplastic diseases of dogs has not been assessed yet. OBJECTIVES: The aim of the present study was to assess the diagnostic accuracy of rapid ICC compared to standard immunohistochemistry (IHC)...
February 10, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28185957/a-three-dimensional-assemblage-of-gingiva-derived-mesenchymal-stem-cells-and-no-releasing-microspheres-for-improved-differentiation
#6
Shobha Regmi, Jiafu Cao, Shiva Pathak, Biki Gupta, Bijay Kumar Poudel, Pham Thanh Tung, Simmyung Yook, Jun-Beom Park, Chul Soon Yong, Jong Oh Kim, Jin-Wook Yoo, Jee-Heon Jeong
Stem cell therapy is an attractive approach to bone tissue regeneration. Nitric oxide (NO) has been reported to facilitate osteogenic differentiation of stem cells. To enhance osteogenic differentiation of gingiva-derived mesenchymal stem cells (GMSCs), we designed a method for in situ delivery of exogenous NO to these cells. A NO donor, polyethylenimine/NONOate, was incorporated into poly(lactic-co-glycolic acid) microspheres to deliver NO to the cells for an extended period of time under in vitro culture conditions...
February 6, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28183011/semi-degradable-porous-poly-vinyl-alcohol-hydrogel-scaffold-for-cartilage-repair-evaluation-of-the-initial-and-cell-cultured-tribological-properties
#7
Yi Cao, Dangsheng Xiong, Kun Wang, Yuxiang Niu
Tissue engineering for articular cartilage repair has shown success in ensuring the integration of neocartilage with surrounding tissue, but the rapid restoration of biomechanical and biotribological functions remains a significant challenge. Poly (vinyl alcohol) (PVA) hydrogel is regarded as a potential articular cartilage replacement for its fair mechanical strength and low surface friction, while its lack of bioactivity limits its utility. Combining the advantages of tissue engineering materials and PVA hydrogel, we developed a semi-degradable porous PVA hydrogel through addition of ploy (lactic-co-glycolic acid) (PLGA) microspheres and salt-leaching technique...
February 2, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28174779/improving-gelation-efficiency-and-cytocompatibility-of-visible-light-polymerized-thiol-norbornene-hydrogels-via-addition-of-soluble-tyrosine
#8
Han Shih, Hung-Yi Liu, Chien-Chi Lin
Hydrogels immobilized with biomimetic peptides have been used widely for tissue engineering and drug delivery applications. Photopolymerization has been among the most commonly used techniques to fabricate peptide-immobilized hydrogels as it offers rapid and robust peptide immobilization within a crosslinked hydrogel network. Both chain-growth and step-growth photopolymerizations can be used to immobilize peptides within covalently crosslinked hydrogels. A previously developed visible light mediated step-growth thiol-norbornene gelation scheme has demonstrated efficient crosslinking of hydrogels composed of an inert poly(ethylene glycol)-norbornene (PEGNB) macromer and a small molecular weight bis-thiol linker, such as dithiothreitol (DTT)...
February 8, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28163358/mirrored-stainless-steel-substrate-provides-improved-signal-for-raman-spectroscopy-of-tissue-and-cells
#9
Aaran T Lewis, Riana Gaifulina, Martin Isabelle, Jennifer Dorney, Mae L Woods, Gavin R Lloyd, Katherine Lau, Manuel Rodriguez-Justo, Catherine Kendall, Nicholas Stone, Geraint M Thomas
Raman spectroscopy (RS) is a powerful technique that permits the non-destructive chemical analysis of cells and tissues without the need for expensive and complex sample preparation. To date, samples have been routinely mounted onto calcium fluoride (CaF2) as this material possesses the desired mechanical and optical properties for analysis, but CaF2 is both expensive and brittle and this prevents the technique from being routinely adopted. Furthermore, Raman scattering is a weak phenomenon and CaF2 provides no means of increasing signal...
January 2017: Journal of Raman Spectroscopy: JRS
https://www.readbyqxmd.com/read/28163228/a-novel-in-situ-silver-hyaluronan-bio-nanocomposite-fabrics-for-wound-and-chronic-ulcer-dressing-in-vitro-and-in-vivo-evaluations
#10
A M Abdel-Mohsen, J Jancar, R M Abdel-Rahman, L Vojtek, P Hyršl, M Dušková, H Nejezchlebová
In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag(0) and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively...
February 3, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28157763/effect-of-procedural-related-variables-on-melanocyte-keratinocyte-suspension-transplantation-in-nonsegmental-stable-vitiligo-a-clinical-and-immunocytochemical-study
#11
Bakr Mohamed El-Zawahry, Samia Esmat, Dalia Bassiouny, Naglaa Sameh Zaki, Rehab Sobhi, Marwah A Saleh, Dalia Abdel-Halim, Rehab Hegazy, Heba Gawdat, Nesrin Samir, Marwa El-Hawary, Zeinab El Maadawi, Heba Gouda, Mervat Khorshied
BACKGROUND: Melanocyte-keratinocyte suspension (M-K susp) is gaining popularity for vitiligo treatment. Few studies have addressed procedure-related variables. OBJECTIVE: To assess the effect of different M-K susp procedure-related variables on the clinical outcome in stable vitiligo. METHODS: This prospective multicenter comparative study included 40 cases with nonsegmental stable vitiligo. Donor site was either a skin graft in noncultured epidermal cell suspension (NCECS) or hair follicle units in outer root sheath hair follicle suspension (ORSHFS)...
February 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28154861/application-of-imaging-mass-spectrometry-approaches-to-facilitate-metal-based-anticancer-drug-research
#12
Ronald F S Lee, Sarah Theiner, Anders Meibom, Gunda Koellensperger, Bernhard K Keppler, Paul J Dyson
Mass spectrometry imaging is being increasingly used in metal-based anticancer drug development to study elemental and/or molecular drug distributions in different biological systems. The main analytical tools employed are SIMS (especially nanoSIMS), LA-ICP-MSI and MALDI-MSI as well as a combination of complementary imaging techniques. Main challenges are appropriate sample preparation methods, reliable and validated quantification strategies and a trade-off between sensitivity and spatial resolution. So far, research has mostly focused on the development of analytical methods for imaging with the long term goal to study drug uptake into tumor tissue and toxicity affected organs and to identify cellular targets of metal-based drugs...
February 3, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28150197/preparation-and-characterization-of-magnetic-nano-in-microparticles-for-pulmonary-delivery
#13
Amber A McBride, Dominique N Price, Pavan Muttil
The purpose of this chapter is to detail the formulation and characterization of a magnetically-targeted drug delivery vehicle, termed nano-in-microparticles (NIMs), for pulmonary drug delivery. Currently, chemotherapeutics and antibiotics are delivered systemically and result in whole body side-effects. NIMs are formulated with superparamagnetic iron oxide nanoparticles, termed SPIONs, making these particles targetable to specific lung regions using a strong external magnet. Additionally, these particles can be formulated to contain any drug or therapeutic agent, such that a therapeutic dose can be delivered to a specific tissue location using the SPIONs-magnet interaction...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28140669/surgical-repositioning-of-the-premaxilla-using-a-minimally-invasive-endonasal-approach
#14
Nicolas E Sierra, Ada Ferrer-Fuertes, Bárbara Salazar-Perez, Gui-Youn Cho-Lee, Carles Martí-Pages, Alejandro Rivera-Baró, Josep Rubio Palau, Eloy M García-Diez
OBJECTIVE:   The aim of this study was to evaluate a technique for the surgical repositioning of the premaxilla using a minimally invasive endonasal approach. DESIGN:   Retrospective review of clinical records. SETTING:   Tertiary care, University Hospital, pediatric maxillofacial surgery unit. PATIENTS:   Twenty-one patients (12 boys and 9 girls), ages ranging from 6 to 21 years, with BCLP+A and premaxillary malposition (PM)...
January 31, 2017: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/28137220/multiwalled-carbon-nanotube-chitosan-scaffold-cytotoxic-apoptotic-and-necrotic-effects-on-chondrocyte-cell-lines
#15
Sibel Ilbasmis-Tamer, Hakan Çiftçi, Mustafa Tu Rk, Tuncer Degim, Uğur Tamer
BACKGROUND: Carbon nanotubes (CNTs) have been considered highly successful and proficient in terms of their mechanical, thermal and electrical functionalization and biocompatibility. In regards to their significant extent in bone regeneration, it has been determined that CNTs hold the capability to endure clinical applications through bone tissue engineering and orthopedic procedures. In the present study, we report on a composite preparation, involving the use of CNT-chitosan as scaffold for bone repair and regeneration...
January 26, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28135787/sirna-loaded-liposomes-inhibition-of-encystment-of-acanthamoeba-and-toxicity-on-the-eye-surface
#16
Kathrin Faber, Giovanni K Zorzi, Nathaly T Brazil, Marilise B Rott, Helder F Teixeira
: Current treatments for Acanthamoeba keratitis are unspecific. Due to the presence of the resilient cyst form of the parasite, the infection is persistent. Silencing the key protein of cyst formation, glycogen phosphorylase, has shown potential for reducing encystment processes of the Acanthamoeba trophozoite. However a suitable carrier to protect and deliver siRNA sequences is still needed. DOTAP: DOPE:DSPE-PEG liposomes were prepared by three different techniques and used to associate a therapeutic siRNA sequence...
January 30, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28135637/fabrication-and-characterization-of-baghdadite-nanostructured-scaffolds-by-space-holder-method
#17
Sorour Sadeghzade, Fateme Shamoradi, Rahmatollah Emadi, Fariborz Tavangarian
Porous baghdadite scaffold has received great attention as a candidate for bone tissue engineering application due to its remarkable bioactivity, biocompatibility, and good bone formation ability. A few studies have been focused on improving the mechanical properties of baghdadite scaffolds. Recently, space holder method has been introduced as a new and viable technique to prepare bioceramic scaffolds with interconnected pores and suitable mechanical properties. In this study, for the first time, 3D baghdadite scaffolds with interconnected porosity were produced using space holder method...
January 24, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28135032/rapid-vacuum-sintering-a-novel-technique-for-fabricating-fluorapatite-ceramic-scaffolds-for-bone-tissue-engineering
#18
Isabelle Denry, Ourania-Menti Goudouri, Jeffrey Harless, Julie A Holloway
Macroporous bioceramic scaffolds are often fabricated via the foam replica technique, based on polymeric foam impregnation with a glass slurry, followed by slow heat treatment to allow for drying, polymeric burnout, and sintering of the glass particles. As a consequence, the process is time consuming and complicated by concurrent crystallization of the glass, often leading to incomplete sintering. Our goal was to investigate the effect of heating rate on sintering behavior, architecture, and mechanical properties of fluorapatite-based glass and glass-ceramic scaffolds...
January 30, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28131907/3d-late-gadolinium-enhanced-cardiovascular-mr-with-centra-plus-profile-view-ordering-feasibility-of-right-ventricular-myocardial-damage-assessment-using-a-swine-animal-model
#19
Keigo Kawaji, Akiko Tanaka, Mita B Patel, Hui Wang, Francesco Maffessanti, Takeyoshi Ota, Amit R Patel
AIMS: To develop a high-resolution, 3D late gadolinium enhancement (LGE) cardiovascular magnetic resonance imaging (MRI) technique for improved assessment of myocardial scars, and evaluate its performance against 2D breath-held (BH) LGE MRI using a surgically implanted animal scar model in the right ventricle (RV). METHODS AND RESULTS: A k-space segmented 3D LGE acquisition using CENTRA-PLUS (Contrast ENhanced Timing Robust Acquisition with Preparation of LongitUdinal Signal; or CP) ordering is proposed...
January 25, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28129447/the-comparative-effectiveness-and-safety-of-three-activated-irrigation-techniques-in-the-isthmus-area-using-a-transparent-tooth-model
#20
A Malentacca, U Uccioli, F Mannocci, B Bhuva, D Zangari, C Pulella, C Lajolo
AIM: To compare the effectiveness and safety of three activated irrigation techniques when removing pulp tissue from the isthmus of a transparent tooth model. The three techniques assessed were: the EndoVac (EV), passive ultrasonic irrigation (PUI) and ultrasonic wave aspiration (TUWA). Conventional syringe irrigation (CSI) was used as a control. METHODOLOGY: A transparent tooth model was created using the mesial root of an extracted mandibular first molar that had an isthmus and two independent mesial canals...
January 27, 2017: International Endodontic Journal
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