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Tissue Preparation techniques

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https://www.readbyqxmd.com/read/29145527/evaluation-of-operating-time-and-patient-perception-using-conventional-impression-taking-and-intraoral-scanning-for-crown-manufacture-a-split-mouth-randomized-clinical-study
#1
Yasser Haddadi, Golnosh Bahrami, Flemming Isidor
PURPOSE: To compare operating time and patient perception of conventional impression (CI) taking and intraoral scanning (IOS) for manufacture of a tooth-supported crown. MATERIALS AND METHODS: A total of 19 patients needing indirect full-coverage restorations fitting the requirements for a split-mouth design were recruited. Each patient received two lithium disilicate crowns, one manufactured from CI taking and one from IOS. Both teeth were prepared following the manufacturers' recommendations...
November 16, 2017: International Journal of Prosthodontics
https://www.readbyqxmd.com/read/29137121/human-mesenchymal-stem-cells-growth-and-osteogenic-differentiation-on-piezoelectric-poly-vinylidene-fluoride-microsphere-substrates
#2
R Sobreiro-Almeida, M N Tamaño-Machiavello, E O Carvalho, L Cordón, S Doria, L Senent, D M Correia, C Ribeiro, S Lanceros-Méndez, R Sabater I Serra, J L Gomez Ribelles, A Sempere
The aim of this work was to determine the influence of the biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture supports were prepared with structures ranging between 2D and 3D, based on PVDF films on which PVDF microspheres were deposited with varying surface density. Maintenance of multipotentiality when cultured in expansion medium was studied by flow cytometry monitoring the expression of characteristic hMSCs markers, and revealed that cells were losing their characteristic surface markers on these supports...
November 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29136629/modified-alcohol-formalin-cell-block-technique-in-head-and-neck-pathology-diagnosis
#3
Karishma M Desai, Punnya V Angadi, Alka D Kale, Seema Hallikerimath
OBJECTIVES: Cell block preparation is a recognized technique in histopathological diagnosis. This technique aids in maintaining an intact architecture and reducing the diagnostic errors associated with fine-needle aspiration cytology. Numerous techniques have been put forth, but the need for an optimal technique for routine use in the laboratory still persists. The aim of our study was to establish a cell block technique which aids in the accurate diagnosis of head and neck pathology...
November 15, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29134236/-arthroscopic-soft-tissue-stabilization-of-posttraumatic-anterior-shoulder-instability-techniques-limitations-and-long-term-results
#4
REVIEW
B Ockert, N Biermann, W Nebelung, E Wiedemann
Arthroscopic soft tissue stabilization is a well-established and broadly accepted procedure to treat posttraumatic shoulder instability. Advantages in comparison to open stabilization procedures include improved visualization of the structural damage and a less invasive approach. Technical developments in recent years have led to further improvement of the arthroscopic technique for shoulder instability. This article presents a description of principles and new developments as it contains basic techniques including patient positioning, access portals, preparation of the glenoid bone, soft tissue handling and shuttle techniques...
November 13, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/29123393/antioxidant-and-hepatoprotective-role-of-selenium-against-silver-nanoparticles
#5
Sabah Ansar, Saad M Alshehri, Manal Abudawood, Sherifa S Hamed, Tansir Ahamad
Silver nanoparticles (AgNPs) have attracted the most interest in terms of their potential biomedical and industrial applications. However, these nanoparticles have shown their toxic behavior toward environment, living tissues, and organisms. Selenium (Se), an essential trace element, is necessary for various metabolic processes, including protection against oxidative stress and immune function. The present study was undertaken to evaluate the effect of Se against AgNP-induced hepatic oxidative stress. AgNPs were synthesized and then prepared nanoparticles were characterized using various analytical techniques such as UV-visible spectroscopy, X-ray diffraction, and transmission electron microscopy...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29123109/label-free-automated-three-dimensional-imaging-of-whole-organs-by-microtomy-assisted-photoacoustic-microscopy
#6
Terence T W Wong, Ruiying Zhang, Chi Zhang, Hsun-Chia Hsu, Konstantin I Maslov, Lidai Wang, Junhui Shi, Ruimin Chen, K Kirk Shung, Qifa Zhou, Lihong V Wang
Three-dimensional (3D) optical imaging of whole biological organs with microscopic resolution has remained a challenge. Most versions of such imaging techniques require special preparation of the tissue specimen. Here we demonstrate microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquires serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue staining or clearing, mPAM generates micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids...
November 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/29122713/fabrication-and-characterization-of-nanobiocomposite-scaffold-of-zein-chitosan-nanohydroxyapatite-prepared-by-freeze-drying-method-for-bone-tissue-engineering
#7
Zeinab Shahbazarab, Abbas Teimouri, Alireza Najafi Chermahini, Mohammad Azadi
In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios. The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique. Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied...
November 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29119943/an-appraisal-on-various-methods-of-nano-particulate-formulations
#8
Bindu Dhiman, Divtrannum Bhalla, Anju Dhiman, Sapna Saini
Nanotechnology has gained a great deal of public interest due to the needs and applications of nanomaterials in many areas of human endeavours such as industry, agriculture, business, medicine, public health amongst many others. Nanotechnology includes the integration of these nanoscale structures into larger material components and systems, keeping the control and construction of new and improved materials at the nanoscale. Biological nanoparticles are mainly developed for drug delivery systems as an alternative to liposomal technology, in order to overcome the problems related to the stability of these vesicles in biological fluids and during the storage...
November 8, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/29117504/experimentally-designed-lyophilized-dry-emulsion-tablets-for-enhancing-the-antihyperlipidemic-activity-of-atorvastatin-calcium-preparation-in-vitro-evaluation-and-in-vivo-assessment
#9
Alaa H Salama, Mona Basha, Sally El Awdan
This article presents the development of lyophilized orally disintegrating tablets prepared with the dry emulsion technique to enhance the in-vitro dissolution and in-vivo performance of the poorly bioavailable drug atorvastatin calcium (ATV). Tablets were fabricated by freeze-drying o/w emulsions of ATV. The Emulsions were prepared using a matrix former solution (alginate or gelatin, 2 or 4%) containing a sugar alcohol (mannitol) and a collapse protectant (glycine) as the water phase and Labrafac® as the oil phase in the presence of surfactant (synperonic® PE/P 84 or synperonic® F108) under proper homogenization...
November 5, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29113084/synergy-effect-of-combining-fluorescence-and-mid-infrared-fiber-spectroscopy-for-kidney-tumor-diagnostics
#10
Andrey Bogomolov, Valeria Belikova, Urszula J Zabarylo, Olga Bibikova, Iskander Usenov, Tatiana Sakharova, Hans Krause, Olaf Minet, Elena Feliksberger, Viacheslav Artyushenko
Matching pairs of tumor and non-tumor kidney tissue samples of four patients were investigated ex vivo using a combination of two methods, attenuated total reflection mid infrared spectroscopy and fluorescence spectroscopy, through respectively prepared and adjusted fiber probes. In order to increase the data information content, the measurements on tissue samples in both methods were performed in the same 31 preselected positions. Multivariate data analysis revealed a synergic effect of combining the two methods for the diagnostics of kidney tumor compared to individual techniques...
November 5, 2017: Sensors
https://www.readbyqxmd.com/read/29110690/autologous-cell-derived-tissue-engineered-cartilage-for-repairing-articular-cartilage-lesions-in-the-knee-study-protocol-for-a-randomized-controlled-trial
#11
Ning Ma, Hongxia Wang, Xian Xu, Yiqun Wan, Yufeng Liu, Mingjie Wang, Wen Yu, Yongjing Dai, Jiang Peng, Quanyi Guo, Changlong Yu, Shibi Lu
BACKGROUND: Spontaneous recovery from articular cartilage injury is difficult, and the ongoing progression of disease can eventually lead to osteoarthritis. Currently, there is no effective non-surgical treatment for articular cartilage injury. Arthroscopic debridement and microfracture surgery are performed for fibrocartilage repair. But fibrocartilage is different from normal articular cartilage, and functional recovery is not satisfactory. Therefore, it is necessary to develop more effective techniques for articular cartilage repair...
November 6, 2017: Trials
https://www.readbyqxmd.com/read/29109319/histological-evaluation-of-the-effect-of-platelet-rich-plasma-on-pulp-regeneration-in-nonvital-open-apex-teeth-an-animal-study
#12
Jamileh Ghoddusi, Amir Maghsudlu, Hamid Jafarzadeh, Amirhossein Jafarian, Maryam Forghani
AIM: Platelet-rich plasma (PRP), which is a concentration of growth factors found in platelets, may be a suitable material for pulp regeneration. The aim of this animal study was a histological evaluation of PRP on pulp regeneration in nonvital teeth with immature apices. MATERIALS AND METHODS: A total of 40 premolar dogs' teeth were chosen for this study. After general anesthesia, the teeth were exposed, and subsequently, pulps were removed and the cavities were opened to the oral cavity...
November 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29101870/enhanced-biological-properties-of-biomimetic-apatite-fabricated-polycaprolactone-chitosan-nanofibrous-bio-composite-for-tendon-and-ligament-regeneration
#13
Geng Wu, Xuefeng Deng, Jinqi Song, Feiqiang Chen
The development of tailored nanofibrous scaffolds for tendon and ligament tissue engineering has been a goal of clinical research for current researchers. Here, we establish a formation of novel nanofibrous matrix with significant mechanical and biological properties by electro-spinning process. The fine fibrous morphology of the nanostructured hydroxyapatite (HAp) dispersed in the polycaprolactone/chitosan (HAp-PCL/CS) nanofibrous matrix was exhibited by microscopic (SEM and TEM) techniques. The favorable mechanical properties (load and modulus) were achieved...
October 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29100989/single-shot-late-gd-enhancement-imaging-of-myocardial-infarction-with-retrospectively-adjustable-contrast-and-heart-phase
#14
Tobias Wech, Johannes Tran-Gia, Felix Rützel, Thorsten Klink, Thorsten A Bley, Herbert Köstler
OBJECT: To enable a retrospective adjustment of image contrast and heart phase in inversion recovery prepared late gadolinium enhancement (LE) imaging in the myocardium. MATERIALS AND METHODS: After one inversion pulse, unsegmented data were acquired over multiple cardiac cycles using a radial spoiled gradient-echo sequence with golden angle increments between subsequent readouts. Model-based acceleration of parameter mapping (MAP) was combined with an image registration technique ("MOCO-MAP") to enable the reconstruction of images with arbitrary inversion time TI...
October 31, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/29098076/intraluminal-ileal-tumour-after-right-hemicolectomy-for-cancer-an-implantation-recurrence-or-a-new-cancer-a-case-report
#15
Marco Clementi, Sara Colozzi, Mario Schietroma, Federico Sista, Andrea Della Penna, Alessandro Chiominto, Stefano Guadagni
Introduction: Extra-anastomotic intraluminal recurrence of the colon cancer after curative surgery was rarely reported but intraluminal ileal relapse has not been described to date.We report a case of intraluminal ileal tumor arising after curative right hemicolectomy that could be ascribed to an implantation of exfoliated cancer cells. Case report: A 71-years old man was admitted with no metastatic stenotic adenocarcinoma of the hepatic flexure and submitted, without preoperative bowel preparation, to right hemicolectomy using a "no-touch" technique...
November 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29092105/formation-and-mosaicity-of-coccolith-segment-calcite-of-the-marine-algae-emiliania-huxleyi
#16
Xiaofei Yin, Andreas Ziegler, Klemens Kelm, Ramona Hofmann, Philipp Watermeyer, Patrick Alexa, Clarissa Villinger, Ulrich Rupp, Lothar Schlüter, Thorsten Reusch, Erika Griesshaber, Paul Walther, Wolfgang Schmahl
Coccolithophores are one of the most abundant calcium carbonate (CaCO3 ) mineralizing organisms. Coccolithophore biomineralization is a complex and highly regulated process. Biomineralization results in a product that strongly differs in its intricate morphology from the abiogenically produced mineral equivalent. Moreover, unlike extracellularly formed biological carbonate hard tissues, coccolithophore calcite is neither a hybrid composite, nor is it distinguished by a hierarchical microstructure. This is remarkable as the key to optimize crystalline biomaterials for mechanical strength and toughness lies in the composite nature of the biological hard tissue and the utilization of specific microstructures...
November 1, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/29088914/monitoring-dopamine-ex-vivo-during-electrical-stimulation-using-liquid-microjunction-surface-sampling
#17
Emily L Gill, Megan Marks, Richard A Yost, Vinata Vedam-Mai, Timothy Garrett
Liquid-microjunction surface sampling (LMJ-SS) is an ambient ionization technique based on the continuous flow of solvent using an in situ micro-extraction device, in which solvent moves through the probe, drawing in the analytes in preparation for ionization using an electrospray ionization (ESI) source. However, unlike traditional mass spectrometry (MS) techniques it operates under ambient pressure and requires no sample preparation, thereby making it ideal for rapid sampling of thicker tissue sections for electrophysiological and other neuroscientific research studies...
October 31, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29084284/a-novel-multicolor-flow-cytometry-application-for-quantitative-detection-of-receptors-on-vascular-smooth-muscle-cells
#18
Aneta Radziwon-Balicka, Matilda Degn, Sara E Johansson, Karin Warfvinge, Lars Edvinsson
There is a need to develop new techniques for quantitative measurement of receptors expression on particular vasculature cells types. Here, we describe and demonstrate a novel method to measure quantitatively and simultaneously the expression of endothelin B receptor (ETB) on vascular smooth muscle cells (VSMC). We isolated cells from male rat tissues such as: brain pial, brain intraparenchymal and retina vessels. To analyze solid tissues, a single-cell suspension was prepared by a combined mechanic and enzymatic process...
2017: PloS One
https://www.readbyqxmd.com/read/29082569/deep-facial-mycosis-due-to-trichophyton-verrucosum-molecular-genetic-identification-of-the-dermatophyte-in-paraffin-embedded-tissue-case-report-and-review-of-the-literature
#19
REVIEW
U Wollina, G Hansel, S Uhrlaß, C Krüger, J Schönlebe, U-C Hipler, P Nenoff
Deep trichophytosis is relatively uncommon. The infection of the bearded area is also known as sycosis barbae or tinea barbae and can be caused by various fungal species, most often zoophilic fungi. We report on an 80-year-old male patient with severe sycosis barbae who had no animal contact and was treated with systemic antibiosis without improvement. Microbial and mycological investigations using swabs from oozing lesions revealed Staphylococcus hemolyticus and Candida parapsilosis. Histology demonstrated fungal elements in hair follicles...
October 30, 2017: Mycoses
https://www.readbyqxmd.com/read/29081372/multiphoton-microscopy-a-novel-diagnostic-method-for-solid-tumors-in-a-prospective-pediatric-oncologic-cohort-an-experimental-study
#20
Oliver J Muensterer, Sibylle Waldron, Yoon Jung Boo, Claudius Ries, Lisa Sehls, Frank Simon, Larissa Seidmann, Jérôme Birkenstock, Jan Gödeke
BACKGROUND: The prognosis of solid pediatric tumors strongly correlates with accurate staging and complete local control. Currently, surgeons rely on macroscopic cues and intraoperative cryosection to determine resection borders. Multiphoton Microscopy (MPM) is a real time technique that allows imaging of tissue without time-consuming tissue processing. PURPOSE: This pilot study evaluates the diagnostic potential of MPM in pediatric solid tumors compared to routine histopathology...
October 30, 2017: International Journal of Surgery
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