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Tissue Preparation techniques

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https://www.readbyqxmd.com/read/28727170/entire-papilla-preservation-technique-in-the-regenerative-treatment-of-deep-intrabony-defects-1-year-results
#1
Serhat Aslan, Nurcan Buduneli, Pierpaolo Cortellini
AIM: This study evaluates the clinical performance of a novel tunnel-like surgical technique in the treatment of isolated deep intrabony defects. MATERIAL AND METHODS: Twelve patients presenting with at least one isolated deep intrabony defect received regenerative periodontal treatment with "entire papilla preservation" technique. Access to the intrabony defect for debridement was provided by a bevelled vertical releasing incision positioned in the buccal gingiva of the neighbouring interdental space...
July 20, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28725319/autologous-stromal-vascular-fraction-in-the-intravenous-treatment-of-end-stage-chronic-obstructive-pulmonary-disease-a-phase-i-trial-of-safety-and-tolerability
#2
Kristin Comella, Jesus A Perez Blas, Tom Ichim, Javier Lopez, Jose Limon, Ruben Corral Moreno
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a consistently progressive, ultimately fatal disease for which no treatment exists capable of either reversing or even interrupting its course. It afflicts more than 5% of the population in many countries, and it accordingly represents the third most frequent cause of death in the US, where it accounts for more than 600 billion in health care costs, morbidity, and mortality. Adipose tissue contains within its stromal compartment a high abundance of adipose stem/stromal cells (ASCs), which can be readily separated from the adipocyte population by methods which require less than 2 h of processing time and yield a concentrated cellular preparation termed the stromal vascular fraction (SVF)...
August 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/28717791/residual-root-preparation-%C3%A2-for-socket-shield-procedures-%C3%A2-a-facial-window-approach
#3
Phillip Roe, Joseph Y K Kan, Kitichai Rungcharassaeng
Two common complications that occur following immediate implant placement and provisionalization procedures in the anterior maxilla are changes to the facial periimplant soft tissue levels and the facial contours. To counteract these changes, different techniques and treatments have been recommended. The recently introduced socket-shield technique (SST) appears to be a viable treatment option for stabilizing the facial osseous and gingival architecture; however, preparation of the root fragment can present challenges...
2017: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/28714105/spinal-cord-imaging-using-averaged-magnetization-inversion-recovery-acquisitions
#4
Matthias Weigel, Oliver Bieri
PURPOSE: To establish a novel approach for fast high-resolution spinal cord (SC) imaging using averaged magnetization inversion recovery acquisitions (AMIRA). METHODS: The AMIRA concept is based on an inversion recovery (IR) prepared, segmented, and time-limited cine balanced steady state free precession sequence. Typically, for the fastest SC imaging without any signal averaging, eight consecutive images in time with an in-plane resolution of 0.67 × 0.67 mm(2) and 6 mm to 8 mm slice thickness are acquired in 51 s...
July 16, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28713743/bone-ring-augmentation-around-immediate-implants-a-clinical-and-radiographic-study
#5
Girish B Giraddi, Aamir Malick Saifi
PURPOSE: The purpose of this study was to evaluate the efficacy of bone ring augmentation technique for three-dimensional augmentation of severely defective sockets along with placement of immediate dental implants. MATERIALS AND METHODS: A clinical study was conducted on 14 patients with 15 defective sockets requiring extractions and immediate implant placement. Following extraction, bone ring with an implant osteotomy, harvested from chin, was sculptured and fitted into the extraction socket...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28713114/retrospective-assessment-of-healing-outcome-of-endodontic-treatment-for-mandibular-molars-with-c-shaped-root-canal
#6
Anika Bansal, Anuj Singh Parihar, Achla Sethi, Kishore Kumar Majety, Jaina Panjabi, Basanta Kumar Choudhury
INTRODUCTION: A thorough knowledge of the anatomic morphology of the root canal system is necessary for the long-term success of the root canal therapy. The occurrence of C-shaped root canal configuration is one such variation. Achievement of favorable prognosis after commencing root canal therapy in such teeth is one of the challenges imposed on the endodontist. Hence, we evaluated the healing occurring after endodontic therapy in patients with C-shaped root canals in mandibular molars...
July 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28711788/grapevine-tissues-and-phenology-differentially-affect-soluble-carbohydrates-determination-by-capillary-electrophoresis
#7
Daniela Moreno, Federico Berli, Rubén Bottini, Patricia N Piccoli, María F Silva
Soluble carbohydrates distribution depends on plant physiology and, among other important factors, determines fruit yield and quality. In plant biology, the analysis of sugars is useful for many purposes, including metabolic studies. Capillary electrophoresis (CE) proved to be a powerful green separation technique with minimal sample preparation, even in complex plant tissues, that can provide high-resolution efficiency. Matrix effect refers to alterations in the analytical response caused by components of a sample other than the analyte of interest...
July 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28711618/fabrication-of-bi-layer-scaffold-of-keratin-nanofiber-and-gelatin-methacrylate-hydrogel-implications-for-skin-graft
#8
Jong Wook Kim, Min Jin Kim, Chang Seok Ki, Hyun Jeong Kim, Young Hwan Park
Bi-layer scaffold composed of human hair keratin/chitosan nanofiber mat and gelatin methacrylate (GelMA) hydrogel was fabricated by using electrospinning and photopolymerization techniques. To prepare the nanofiber layer, the blend solution of human hair keratin and chitosan (mixture ratio: 5/5) was electrospun using formic acid as a solvent in the presence of poly(ethylene glycol), followed by cross-linking with glutaraldehyde. The tensile strength of the human hair keratin/chitosan nanofiber mat was much higher than that of pure human hair keratin nanofiber mat...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28711017/alginate-gelatin-scaffolds-incorporated-with-silibinin-loaded-chitosan-nanoparticles-for-bone-formation-in-vitro
#9
R S Leena, M Vairamani, N Selvamurugan
Silibinin is a plant derived flavonolignan known for its multiple biological properties, but its role in the promotion of bone formation has not yet been well studied. Moreover, the delivery of Silibinin is hindered by its complex hydrophobic nature, which limits its bioavailability. Hence, in this study, we fabricated a drug delivery system using chitosan nanoparticles loaded with Silibinin at different concentrations (20μM, 50μM, and 100μM). They were then incorporated into scaffolds containing Alginate and Gelatin (Alg/Gel) for the sustained and prolonged release of Silibinin...
July 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28707422/description-of-d-glucosamine-immobilization-kinetics-onto-poly-lactic-acid-surface-via-a-multistep-physicochemical-approach-for-preparation-of-novel-active-biomaterials
#10
Ahmed E Swilem, Marian Lehocký, Petr Humpolíček, Zdenka Kucekova, Igor Novák, Matej Mičušík, Hassan A Abdel-Rehim, El-Sayed A Hegazy, Ashraf A Hamed, Jaroslav Kousal
Poly(lactic acid) (PLA) has shown much success in the preparation of tissue engineering scaffolds as it can be fabricated with a tailored architecture. However, the PLA surface has drawbacks including the lack of biofunctional motifs which are essential for high affinity to biological cells. Therefore, this study describes a multistep physicochemical approach for the immobilization of D-glucosamine (GlcN), a naturally occurring monosaccharide having many biological functions, on the PLA surface aiming at enhancing the cell proliferation activity...
July 13, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28706811/surgical-technique-for-arthroscopy-assisted-anatomical-reconstruction-of-acromioclavicular-and-coracoclavicular-ligaments-using-autologous-hamstring-graft-in-chronic-acromioclavicular-joint-dislocations
#11
Bancha Chernchujit, Prashant Parate
Injuries to the acromioclavicular (AC) joint are becoming common with contact sports and bike accidents. It is well known that in AC dislocations, the first structure to fail is the AC capsule followed by the trapezoid and conoid ligaments. The function of these ligaments must be restored to restore the anatomy and physiology of the AC joint to get the best results. Until now, no technique has emerged as the gold standard for restoration of the AC joint anatomy and function. In our technique, the stress is on recreating the anatomy to make it more individualized based on individual variations...
June 2017: Arthroscopy Techniques
https://www.readbyqxmd.com/read/28706272/modeling-atrial-fibrillation-using-human-embryonic-stem-cell-derived-atrial-tissue
#12
Zachary Laksman, Marianne Wauchop, Eric Lin, Stephanie Protze, Jeehoon Lee, Wallace Yang, Farzad Izaddoustdar, Sanam Shafaattalab, Lior Gepstein, Glen F Tibbits, Gordon Keller, Peter H Backx
Since current experimental models of Atrial Fibrillation (AF) have significant limitations, we used human embryonic stem cells (hESCs) to generate an atrial-specific tissue model of AF for pharmacologic testing. We generated atrial-like cardiomyocytes (CMs) from hESCs which preferentially expressed atrial-specific genes, and had shorter action potential (AP) durations compared to ventricular-like CMs. We then generated confluent atrial-like CM sheets and interrogated them using optical mapping techniques. Atrial-like CM sheets (~1 cm in diameter) showed uniform AP propagation, and rapid re-entrant rotor patterns, as seen in AF could be induced...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705112/pcl-tcp-wet-spun-scaffolds-carrying-antibiotic-loaded-microspheres-for-bone-tissue-engineering
#13
Elbay Malikmammadov, Tugba Endogan Tanir, Aysel Kiziltay, Vasif Hasirci, Nesrin Hasirci
Scaffolds produced for tissue engineering applications are proven to be promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, porous and fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of tricalcium phosphate (TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investigated against Escherichia coli (E...
July 14, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28702464/a-cost-effective-rna-extraction-technique-from-animal-cells-and-tissue-using-silica-columns
#14
Mario D Escobar, Jason L Hunt
Ribonucleic acid (RNA) is widely used in molecular biology assays, and some of the most common assays include: northern blotting and RT-PCR gene expression analysis. RNA is generally extracted by two methods: phenol-chloroform or commercially available silica spin column kits. Phenol-chloroform extraction is generally more economical; however, it produces hazardous byproducts, and leftover chemicals in the sample that can inhibit downstream applications. Commercial kits usually have simple set ups and short preparation time; however, they can introduce a significant expense to laboratory budgets...
2017: Journal of Biological Methods
https://www.readbyqxmd.com/read/28699619/highly-defined-3d-printed-chitosan-scaffolds-featuring-improved-cell-growth
#15
Lisa Elviri, Ruben Foresti, Carlo Bergonzi, Francesca Zimetti, Cinzia Marchi, Annalisa Bianchera, Franco Bernini, Marco Silvestri, Ruggero Bettini
The augmented demand for medical devices devoted to tissue regeneration and possessing a controlled micro-architecture means there is a need for industrial scale-up in the production of hydrogels. A new 3D printing technique was applied to the automation of a freeze-gelation method for the preparation of chitosan scaffolds with controlled porosity. For this aim, a dedicated 3D printer was built in-house: a preliminary effort has been necessary to explore the printing parameter space to optimize the printing results in terms of geometry, tolerances and mechanical properties of the product...
July 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28699285/itraq-based-proteomic-analysis-reveals-the-roles-of-progesterone-receptor-inflammatory-and-fibrosis-for-slow-transit-constipation
#16
Yuwei Li, Yongjun Yu, Shuyuan Li, Mingqing Zhang, Zhao Zhang, Yang Shi, Xipeng Zhang, Shiwu Zhang
BACKGROUND AND AIM: Progesterone receptor, inflammation, neurotransmitter expression, and fibrosis are involved in slow-transit constipation. The aim of the present study was to examine whether patients with slow-transit constipation have an overexpression of progesterone receptor and serotonin which may impair the fibrosis of muscularis propria in colorectal wall. METHODS: High resolution colon manometry was used to record the colorectal peristaltic contractions of the proximal ascending and sigmoid colon in patients...
July 11, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28698831/effectiveness-of-reverse-sural-artery-flap-in-the-management-of-wheel-spoke-injuries-of-the-heel
#17
Hafiz U Farooq, Rizwan Ishtiaq, Shabana Mehr, Sadia Ayub, Umer H Chaudhry, Anam Ashraf
OBJECTIVE: Soft tissue injuries at the level of lower extremities, plantar, and dorsal foot pose a surgical challenge for reconstructive surgeons. This kind of injury commonly occurs when lower limbs get stuck in between the spokes of the wheel. Reverse sural artery flap has been proven to be an effective option to cover such defects. The aim of this study is to analyze the demographic variables of affected individuals, technical aspects of reverse sural artery flap, quantify the effectiveness of reverse sural artery flap among various treatment options available, and to study the outcome of injury...
June 10, 2017: Curēus
https://www.readbyqxmd.com/read/28696960/sinus-floor-augmentation-with-ambient-blood-and-an-absorbable-collagen-sponge-a-prospective-pilot-clinical-study
#18
Antoine Berberi, Nabih Nader, Rita Bou Assaf, Hussein Fayyad-Kazan, Saad Khairalah, Nabil Moukarzel
PURPOSE: The aim of this study was to clinically, radiologically, and histologically evaluate a sinus augmentation technique using a resorbable collagen sponge to maintain space between the Schneiderian membrane and the residual crestal bone. MATERIALS AND METHODS: Patients with partially edentulous maxillae were clinically and radiographically evaluated for implant placement. A total of 10 consecutive patients with the bone height for implant placement (<4.0 mm) were enrolled in the study...
July 10, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28696558/mammalian-cell-tissue-culture
#19
Katy Phelan, Kristin M May
Cultured mammalian cells are used extensively in the field of human genetics. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2017 by John Wiley & Sons, Inc.
July 11, 2017: Current Protocols in Human Genetics
https://www.readbyqxmd.com/read/28696495/robotic-arm-assisted-total-knee-arthroplasty-demonstrated-soft-tissue-protection
#20
Anton Khlopas, Morad Chughtai, Emily L Hampp, Laura Y Scholl, Michael Prieto, Ta-Cheng Chang, Abdullah Abbasi, Manoshi Bhowmik-Stoker, Jason Otto, David J Jacofsky, Michael A Mont
INTRODUCTION: While total knee arthroplasty (TKA) procedures have demonstrated clinical success, occasionally intraoperative complications can occur. Collateral or posterior cruciate ligament injury, instability, extensor mechanism disruption, and tibiofemoral or patellofemoral dislocation are among a few of the intraoperatively driven adverse events prevalently ranked by The Knee Society. Robotic-arm assisted TKA (RATKA) provides a surgeon the ability to three-dimensionally plan a TKA and use intraoperative visual, auditory, and tactile feedback to ensure that only the desired bone cuts are made...
July 11, 2017: Surgical Technology International
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