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Tissue Preparation techniques

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https://www.readbyqxmd.com/read/28325314/the-use-of-calcium-ions-instead-of-heat-treatment-for-%C3%AE-1-3-glucan-gelation-improves-biocompatibility-of-the-%C3%AE-1-3-glucan-ha-bone-scaffold
#1
Katarzyna Klimek, Agata Przekora, Aleksandra Benko, Wiktor Niemiec, Marta Blazewicz, Grazyna Ginalska
The aim of this study was to determine which procedure for β-1,3-glucan gelation - newly developed dialysis against calcium salt or described in the literature thermal technique - is more appropriate for fabrication of a biomaterial designed for bone tissue engineering applications. Thus, β-1,3-glucan/hydroxyapatite scaffolds were prepared based on two different methods and their physicochemical, microstructural, and biological properties were compared. Obtained results demonstrated that unlike thermal method-prepared β-1,3-glucan/hydroxyapatite material (glu/HAT), bone scaffold fabricated via dialysis method (glu/HA D) possessed rough surface resulting from the presence of CaCl2 precipitates as proven by SEM and EDS analysis...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28324641/preparation-of-a-non-woven-poly-%C3%AE%C2%B5-caprolactone-fabric-with-partially-embedded-apatite-surface-for-bone-tissue-engineering-applications-by-partial-surface-melting-of-poly-%C3%AE%C2%B5-caprolactone-fibers
#2
In Ae Kim, Sang-Hoon Rhee
This paper describes a novel method for the preparation of a biodegradable non-woven poly(ε-caprolactone) fabric with a partially-embedded apatite surface designed for application as a scaffold material for bone tissue engineering. The non-woven poly(ε-caprolactone) fabric was generated by the electro-spinning technique and then apatite was coated in simulated body fluid after coating the PVA solution containing CaCl2 ·2H2 O. The apatite crystals were partially- or fully-embedded into the thermoplastic poly(ε-caprolactone) fibers by controlling the degree of poly(ε-caprolactone) fiber surface melting in a convection oven...
March 21, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28324600/light-sheet-fluorescence-microscopy-chemical-clearing-and-labeling-protocols-for-ultramicroscopy
#3
Nina Jährling, Klaus Becker, Saiedeh Saghafi, Hans-Ulrich Dodt
Light-sheet microscopy is an effective technique in neuroscience, developmental biology, and cancer research for visualizing and analyzing cellular networks and whole organs in three dimensions. Because this technique requires specimens to be translucent they commonly have to be cleared before microscopy inspection. Here, we provide 3DISCO based protocols for preparing cleared samples of immuno-stained neural networks, lectin-labeled vascular networks, and Methoxy-X04 labeled beta-amyloid plaques in mice. 3DISCO utilizes the lipophilic solvents tetrahydrofuran (THF) and dibenzylether (DBE) for dehydration and successive clearing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28322621/bladder-regeneration-using-multiple-scaffolds-with-growth-factors-in-a-bladder
#4
Luc A J Roelofs, Paul K J D de Jonge, Egbert Oosterwijk, Dorien M Tiemessen, Barbara B Kortmann, Robert P E de Gier, Elly M M Versteeg, Willeke F Daamen, Toin H van Kuppevelt, Paul J Geutjes, Wout Feitz
INTRODUCTION: Tissue engineering may become an alternative to current bladder augmentation techniques. Large scaffolds are needed for clinically significant augmentation, but can result in fibrosis and graft shrinkage. The purpose of this study was to investigate the use of multiple scaffolds instead of one large scaffold, to enhance bladder tissue regeneration and bladder capacity. Secondly, acellular collagen, collagen-heparin, and collagen-heparin scaffolds with growth factors were used and the biological activity of the different scaffolds was compared in a large animal model...
March 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28321474/transvaginal-iliococcygeus-fixation-for-posthysterectomy-vaginal-vault-prolapse-repair
#5
Rodolfo Milani, Matteo Frigerio, Federico Spelzini, Stefano Manodoro
INTRODUCTION AND HYPOTHESIS: Posthysterectomy vaginal vault prolapse repair is a surgical challenge. Successful surgical management using native tissue can be achieved via the vaginal approach by iliococcygeus fascia fixation. However, although iliococcygeus fascia fixation is technically simple and has a low morbidity, it is not commonly performed. The aim of the video is to provide anatomic views and the surgical steps necessary to achieve successful transvaginal iliococcygeus fascia fixation for vaginal vault prolapse repair...
March 20, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28315764/development-of-nanocomposite-scaffolds-based-on-tio2-doped-in-grafted-chitosan-hydroxyapatite-by-freeze-drying-method-and-evaluation-of-biocompatibility
#6
Saber Abd-Khorsand, Samaneh Saber-Samandari, Saeed Saber-Samandari
Porous three-dimensional scaffolds with potential for application as cancellous bone graft substitutes were prepared using the freeze-drying technique. Hydroxyapatite with different weight ratio were embedded in the network of poly(acrylic acid) grafted chitosan accompanied by using TiO2 as an auxiliary component to fabricate porous nanocomposite bone scaffolds. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analysis and mechanical tests were carried out to characterize the prepared scaffolds...
March 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28315464/a-novel-nanosuspension-of-andrographolide-preparation-characterization-and-passive-liver-target-evaluation-in-rats
#7
Ligang Guo, Le Kang, Xinrong Liu, Xiangyun Lin, Donghua Di, Yong Wu, Dexian Kong, Yihui Deng, Yanzhi Song
Andrographolide (Andro) is an excellent anti-inflammatory bicyclic diterpene γ-lactone. However, the poor solubility limits its application as injection for the treatment of acute inflammation. To meet the clinical needs for emergency, the Andro nanosuspensions injection was first prepared by the wet milling technique. The Andro nanosuspensions were composed of 3% Andro, 5% poloxamer 188 as the non-ionic stabilizer, 0.05% sodium deoxycholate or 0.1% sodium tauroursodeoxy cholate as the ionic stabilizer, and prepared by 350rpm speed and 12cycles of grinding with 0...
March 14, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28303966/targeted-delivery-of-doxorubicin-by-nano-loaded-mesenchymal-stem-cells-for-lung-melanoma-metastases-therapy
#8
Yuekui Zhao, Shanshan Tang, Jiamin Guo, Murad Alahdal, Shunxiu Cao, Zhaocong Yang, Fangfang Zhang, Yumeng Shen, Minjie Sun, Ran Mo, Li Zong, Liang Jin
Poor antigenic presentation of tumor tissues and a lack of specific targets currently limit the success of nanoparticle delivery system. Cellular carrier technique has been recently explored extensively as a substitutive or supplement for traditional targeting delivery system. Here, we demonstrate the usage of mesenchymal stem cells (MSCs) loaded with doxorubicin containing polymer nanoparticles in pulmonary melanoma metastases therapy, as a modified technique of targeted delivery system. The characterizations of prepared nanoparticles and MSCs sensitivity to DOX and PLGA-DOX were measured...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28301490/a-novel-flexible-microfluidic-meshwork-to-reduce-fibrosis-in-glaucoma-surgery
#9
Behzad Amoozgar, Xiaoling Wei, Jun Hui Lee, Michele Bloomer, Zhengtuo Zhao, Paul Coh, Fei He, Lan Luan, Chong Xie, Ying Han
PURPOSE/RELEVANCE: Fibrosis and hence capsule formation around the glaucoma implants are the main reasons for glaucoma implant failure. To address these issues, we designed a microfluidic meshwork and tested its biocompatibility in a rabbit eye model. The amount of fibrosis elicited by the microfluidic meshwork was compared to the amount elicited by the plate of conventional glaucoma drainage device. METHODS: Six eyes from 3 New Zealand albino rabbits were randomized to receive either the novel microfluidic meshwork or a plate of Ahmed glaucoma valve model PF7 (AGV PF7)...
2017: PloS One
https://www.readbyqxmd.com/read/28293399/up-regulation-of-tlr2-and-tlr4-in-high-mobility-group-box1-stimulated-macrophages-in-pulpitis-patients
#10
Javad Mahmoudi, Babak Sabermarouf, Behzad Baradaran, Leila Sadat-Hatamnezhad, Siamak Sandoghchian Shotorbani
OBJECTIVES: High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293394/evaluation-of-the-expression-of-viii-factor-and-vegf-in-the-regeneration-of-non-vital-teeth-in-dogs-using-propolis
#11
Mina Zarei, Amir Hossein Jafarian, Azadeh Harandi, Maryam Javidi, Maryam Gharechahi
OBJECTIVES: The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog's teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog's premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28288198/risk-for-molecular-contamination-of-tissue-samples-evaluated-for-targeted-anti-cancer-therapy
#12
Eyal Asor, Michael Y Stav, Einav Simon, Ibrahim Fahoum, Edmond Sabo, Ofer Ben-Izhak, Dov Hershkovitz
With the increasing usage of sensitive PCR technology for pharmacogenetics, cross contamination becomes a significant concern. Researchers employed techniques which basically include replacing laboratory equipment after each sample preparation; however, there are no recommended guidelines. In the present work we wanted to evaluate the risk of cross contamination during tissue processing using the routine precaution measures. Twenty-one surgical samples of lung adenocarcinoma were used, of which 7 contained EGFR exon 19 mutation, 7 contained EGFR exon 21 mutation (p...
2017: PloS One
https://www.readbyqxmd.com/read/28285723/epigenetic-aspects-of-rheumatoid-arthritis-contribution-of-non-coding-rnas
#13
REVIEW
Bogdan Kolarz, Maria Majdan
Since microRNA was discovered in the late 1990s the role of non-coding RNAs in the regulation of cellular processes has been confirmed. Intensive researches have revealed a number of subtypes of non-coding RNA. It has been proved that these molecules can influence each step of the development of cells and tissues. Moreover, researchers have found their expression change during disease development. In this article, we gathered the current results on the contribution of microRNA and long non-coding RNA in rheumatoid arthritis pathogenesis and their potential use in rheumatoid arthritis treatment...
January 20, 2017: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/28282669/comparison-of-anterior-cruciate-ligament-graft-isometry-between-paired-femoral-and-tibial-tunnels
#14
E Lyle Cain, Marcus D Biggers, David P Beason, Benton A Emblom, Jeffrey R Dugas
Accurate tunnel placement is important for a successful anterior cruciate ligament (ACL) reconstruction. Controversy exists concerning the preferred method of femoral tunnel preparation, with proponents of both medial portal and transtibial drilling techniques. Current ACL literature suggests that placement of the femoral ACL attachment site posterior or "low" in the ACL footprint leads to more anatomically correct ACL mechanics and better rotational control. There is limited literature focusing on ACL graft displacement through knee range of motion based on specific paired placement of femoral and tibial tunnels...
March 10, 2017: Journal of Knee Surgery
https://www.readbyqxmd.com/read/28280690/optimization-and-validation-of-cryostat-temperature-conditions-for-trans-reflectance-mode-ftir-microspectroscopic-imaging-of-biological-tissues
#15
Sumedha Liyanage, Rohan S Dassanayake, Amal Bouyanfif, Erandathi Rajakaruna, Latha Ramalingam, Naima Moustaid-Moussa, Noureddine Abidi
In Fourier transform infrared (FTIR) microspectrocopy, the tissue preparation method is crucial, especially how the tissue is cryo-sectioned prior to the imaging requires special consideration. Having a temperature difference between the cutting blade and the specimen holder of the cryostat greatly affects the quality of the sections. Therefore, we have developed an optimal protocol for cryo-sectioning of biological tissues by varying the temperature of both the cutting blade and the specimen holder. Using this protocol, we successfully cryo-sectioned four different difficult-to-section tissues including white adipose tissue (WAT), brown adipose tissue (BAT), lung, and liver...
2017: MethodsX
https://www.readbyqxmd.com/read/28279161/methylated-site-display-msd-aflp-a-sensitive-and-affordable-method-for-analysis-of-cpg-methylation-profiles
#16
Toshiki Aiba, Toshiyuki Saito, Akiko Hayashi, Shinji Sato, Harunobu Yunokawa, Toru Maruyama, Wataru Fujibuchi, Hisaka Kurita, Chiharu Tohyama, Seiichiroh Ohsako
BACKGROUND: It has been pointed out that environmental factors or chemicals can cause diseases that are developmental in origin. To detect abnormal epigenetic alterations in DNA methylation, convenient and cost-effective methods are required for such research, in which multiple samples are processed simultaneously. We here present methylated site display (MSD), a unique technique for the preparation of DNA libraries. By combining it with amplified fragment length polymorphism (AFLP) analysis, we developed a new method, MSD-AFLP...
March 9, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28276540/rapid-fabrication-of-functionalised-poly-dimethylsiloxane-microwells-for-cell-aggregate-formation
#17
A Forget, A L S Burzava, B Delalat, K Vasilev, F J Harding, A Blencowe, N H Voelcker
Cell aggregates reproduce many features of the natural architecture of functional tissues, and have therefore become an important in vitro model of tissue function. In this study, we present an efficient and rapid method for the fabrication of site specific functionalised poly(dimethylsiloxane) (PDMS) microwell arrays that promote the formation of insulin-producing beta cell (MIN6) aggregates. Microwells were prepared using an ice templating technique whereby aqueous droplets were frozen on a surface and PDMS was cast on top to form a replica...
March 9, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28274872/robot-assisted-laparoscopic-transplantation-of-frozen-thawed-ovarian-tissue
#18
Kutluk Oktay, Enes Taylan, Yodo Sugishita, Gabriela M Goldberg
STUDY OBJECTIVE: To demonstrate a new approach for orthotopic human ovarian tissue transplantation via robot-assisted laparoscopic surgery. DESIGN: A step-by-step video explanation of the surgical technique (Canadian Task Force Classification-III). SETTING: Although still experimental, cryopreservation and subsequent transplantation of frozen-thawed ovarian tissue is currently the only available method for prepubertal girls and young women with cancer who are not eligible for established fertility preservation options such as the oocyte or embryo cryopreservation (1)...
March 5, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28274068/sectional-impressions-and-every-other-tooth-technique-in-fpd
#19
Swathi Sudhapalli
Gingival displacement procedure is an important component of fixed prosthodontics. It helps in delivery of the impression material subgingivally to record the finish line as well as the tooth contour apical to it, so as to produce restorations with closely adapted margins in harmony with the tooth anatomy and gingiva. While displacing gingiva with retraction cords, the health of the tissues must be maintained. In case of multiple anterior tooth preparations with close root proximity, placing the retraction cord simultaneously around all teeth will result in strangulation of interdental papillae...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28273205/expression-of-e-cadherin-and-involucrin-in-leukoplakia-and-oral-cancer-an-immunocytochemical-and-immunohistochemical-study
#20
Alessandra Dutra da Silva, Bruna Jalfim Maraschin, Natalia Koerich Laureano, Natália Daroit, Fernanda Brochier, Leonardo Bündrich, Fernanda Visioli, Pantelis Varvaki Rados
To assess the immunocytochemical and immunohistochemical correlation of adhesion (E-cadherin) and cell differentiation (involucrin) molecules in oral leukoplakia and oral squamous cell carcinoma. Cytological samples and biopsies were obtained from male and female patients aged over 30 years with oral leukoplakia (n = 30) and oral squamous cell carcinoma (n = 22). Cell scrapings and the biopsy were performed at the site of the lesion and histological slides were prepared for the immunocytochemical analysis of exfoliated oral mucosal cells and for the immunohistochemical analysis of biopsy tissues using E-cadherin and involucrin...
March 6, 2017: Brazilian Oral Research
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