keyword
MENU ▼
Read by QxMD icon Read
search

Tissue handling

keyword
https://www.readbyqxmd.com/read/28329162/the-value-of-fluorine-18-deoxyglucose-positron-emission-tomography-scans-in-patients-with-ventricular-assist-device-specific-infections%C3%A2
#1
Alexander M Bernhardt, Mustafa A Pamirsad, Chantal Brand, Daniel Reichart, Milena Tienken, Markus J Barten, Andreas Schaefer, Hanno Grahn, Meike Rybczynski, Tobias Deuse, Hermann Reichenspurner, Florian M Wagner
BACKGROUND: Infections are major complications in patients with ventricular assist devices (VAD). Positron emission tomography with deoxyglucose marked by fluorine-18 ( 18 F-FDG PET/CT) is a diagnostic tool to scan for tissue with high metabolism as present in infections. The specificity of 18 F-FDG PET/CT to discriminate between infection and an aseptic reaction of the implanted device is not defined and its evaluation is the aim of this retrospective analysis. METHODS: Until September 2015 a total of 100 patients underwent VAD implantations in our institution...
February 20, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28324061/progesterone-protects-against-bpa-induced-arrhythmias-in-female-rat-cardiac-myocytes-via-rapid-signaling
#2
Jianyong Ma, Kui Hong, Hong-Sheng Wang
Bisphenol A (BPA) is an estrogenic endocrine disrupting chemical (EDC) that has a range of potential adverse health effects. Previously we showed that acute exposure to BPA promoted arrhythmias in female rat hearts through estrogen receptor rapid signaling. Progesterone (P4) and estrogen have antagonistic or complementary actions in a number of tissues and systems. In the current study, we examined the influence, and possible protective effect, of P4 on the rapid cardiac actions of BPA in female rat cardiac myocytes...
January 25, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323929/human-brown-adipose-tissue-temperature-and-fat-fraction-are-related-to-its-metabolic-activity
#3
Kalle Koskensalo, Juho Raiko, Teemu Saari, Virva Saunavaara, Olli Eskola, Pirjo Nuutila, Jani Saunavaara, Riitta Parkkola, Kirsi A Virtanen
Background and aim: The metabolic activity of human brown adipose tissue (BAT) has been previously examined using positron-emission-tomography (PET). The aim of this study was to use proton Magnetic Resonance Spectroscopy (1H MRS) to investigate whether the temperature and the fat fraction (FF) of brown and white adipose tissue (WAT) are associated with BAT metabolic activity determined by 18F-FDG-PET. Materials and methods: Ten healthy subjects (4f/6m, 25-45 y) were studied using PET-MRI during acute cold exposure and at ambient room temperature...
February 21, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28322960/sericin-binded-deprotenized-natural-rubber-film-containing-chitin-whiskers-as-elasto-gel-dressing
#4
Anyarat Watthanaphanit, Ratana Rujiravanit
Here, we aims to demonstrate a simple concept in biomaterials design by using natural resources solely as raw materials to fabricate elastic wound care dressing. Elasto-gel films comprise of silk sericin (SRC), natural rubber (NR), and chitin whisker (CTW) were developed. A glue-like protein SRC found in silk cocoons is beneficial for the treatment of wounds due to its superior skin moisturizing ability. However, the pure SRC film is generally difficult to be fabricated because of its weak structural feature...
March 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28321364/effect-of-method-of-deduplication-on-estimation-of-differential-gene-expression-using-rna-seq
#5
Anna V Klepikova, Artem S Kasianov, Mikhail S Chesnokov, Natalia L Lazarevich, Aleksey A Penin, Maria Logacheva
BACKGROUND: RNA-seq is a useful tool for analysis of gene expression. However, its robustness is greatly affected by a number of artifacts. One of them is the presence of duplicated reads. RESULTS: To infer the influence of different methods of removal of duplicated reads on estimation of gene expression in cancer genomics, we analyzed paired samples of hepatocellular carcinoma (HCC) and non-tumor liver tissue. Four protocols of data analysis were applied to each sample: processing without deduplication, deduplication using a method implemented in SAMtools, and deduplication based on one or two molecular indices (MI)...
2017: PeerJ
https://www.readbyqxmd.com/read/28320539/cytotoxicity-biocompatibility-and-biomineralization-of-the-new-high-plasticity-mta-material
#6
Luciano Tavares Angelo Cintra, Francine Benetti, Índia Olinta de Azevedo Queiroz, Juliana Maria de Araújo Lopes, Sandra Helena Penha de Oliveira, Gustavo Sivieri Araújo, João Eduardo Gomes-Filho
INTRODUCTION: Mineral trioxide aggregate (MTA) has excellent biological properties, but its handling properties have been criticized for both ProRoot MTA (Tulsa Dental Products, Tulsa, OK) and white MTA-Angelus (MTA-Ang; Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brazil). Angelus MTA HP (high plasticity) (Angelus Indústria de Produtos Odontológicos S/A) has been introduced recently. Considering the importance of biological properties of materials that will be in contact with the tissues, this study evaluated the cytotoxicity, biocompatibility, and biomineralization of MTA HP compared with white MTA-Ang...
March 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28320052/soft-tissue-infections-from-fish-spike-wounds-normal-commensal-bacteria-are-more-common-than-marine-pathogens
#7
Hannah Collins, Kin Mun Lee, Paul T-Y Cheng, Sarah Hulme
BACKGROUND: A fish spike injury can be sustained by anyone handling fish; during fishing, meal preparation or in retail. Case reports of fish spikes inoculating victims with virulent marine-specific pathogens and causing systemic illness led us to question whether empirical treatment of these injuries with amoxicillin and clavulanic acid is adequate. METHODS: This 2-year prospective observational study was conducted at Middlemore Hospital, Auckland, New Zealand...
March 20, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/28316760/handling-and-pathology-reporting-of-gastrointestinal-endoscopic-mucosal-resection
#8
REVIEW
Bita Geramizadeh, David A Owen
Endoscopic mucosal resection (EMR) is a non-invasive alternative to surgery that is now frequently used for resection of early lesions in both upper and lower parts of the gastrointestinal (GI) tract. One of the main advantages of these techniques is providing tissue for histopathological examination. Pathological examination of endoscopically resected specimens of GI tract is a crucial component of these procedures and is useful for prediction of both the risk of metastasis and lymph node involvement. As the first step, it is very important for the pathologist to handle the EMR gross specimen in the correct way: it should be oriented, and then the margins should be labeled and inked accurately before fixation...
January 2017: Middle East Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28314905/comparison-of-two-and-three-dimensional-display-for-performance-of-laparoscopic-total-gastrectomy-for-gastric-cancer
#9
Shingo Kanaji, Satoshi Suzuki, Hitoshi Harada, Masayasu Nishi, Masashi Yamamoto, Takeru Matsuda, Taro Oshikiri, Tetsu Nakamura, Yasuhiro Fujino, Masahiro Tominaga, Yoshihiro Kakeji
PURPOSE: Introduction of three-dimensional (3D) display might remove technical obstacles of laparoscopic surgery and improve laparoscopic skills. We analyzed the effect of 3D technology on operative performance during laparoscopic total gastrectomy (LTG) for gastric cancer and assessed its advantages over two-dimensional (2D) laparoscopy. METHODS: This study included 30 consecutive surgeries of LTG with esophagojejunostomy by the overlap method performed (3D group, n = 15, 2D group, n = 15)...
March 17, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/28301994/does-preoperative-transarterial-chemoembolization-for-hepatocellular-carcinoma-increase-the-incidence-of-hepatic-artery-thrombosis-after-living-donor-liver-transplant
#10
Volkan Ince, Veysel Ersan, Serdar Karakas, Koray Kutluturk, Nese Karadag, Ramazan Kutlu, Sezai Yilmaz
OBJECTIVES: Transarterial chemoembolization is a potential risk factor for hepatic artery damage, which may lead to severe consequences. We aimed to investigate this controversial issue in our population of liver transplant patients with hepatocellular carcinoma. MATERIALS AND METHODS: Between March 2006 and December 2016, a total of 262 patients with hepatocellular carcinoma underwent liver transplant at our institution. Of these, 22 (8.4%) had preoperative transarterial chemoembolization...
March 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28300011/neonatal-fatty-acid-profiles-are-correlated-with-infant-growth-measures-at-6-months
#11
P F O'Tierney-Ginn, D Davina, M Gillingham, D J P Barker, C Morris, K L Thornburg
Rapid weight gain in infancy and low levels of n-3 long chain polyunsaturated fatty acids (LCPUFA) at birth are associated with increased adiposity later in life. The association between placental LCPUFA delivery and weight gain in infancy is poorly understood. We sought to determine the relationships between maternal phenotype, placental fatty acid transporter expression and offspring growth patterns over the first 6 months. Placental tissue and cord blood were collected at term delivery from women with uncomplicated pregnancies...
March 16, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28299265/the-use-of-buccal-fat-pad-free-graft-in-closure-of-soft-tissue-defects-and-dehiscence-in-the-hard-palate
#12
Fares Kablan
INTRODUCTION: The integrity of the palatal mucosa can be lost due to congenital, pathological, and iatrogenic conditions. Various surgical techniques have been suggested for the closure of palatal defects. The aim of the current study is to present the free buccal fat pad graft as a novel technique to repair the soft-tissue defects at the palate. PATIENTS AND METHODS: During a 2-year period, the free fat tissue graft harvested from the buccal fat pad (BFP) (FBFG) and used to reconstruct five soft-tissue defects of the palate in five patients (2 women, 3 men; mean age, 34 years; range, 22-58 years)...
July 2016: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28297585/electromechanical-conditioning-of-adult-progenitor-cells-improves-recovery-of-cardiac-function-after-myocardial-infarction
#13
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28293872/effect-of-high-laser-output-on-the-central-bronchi-and-pulmonary-artery
#14
A Kirschbaum, P Rexin, D K Bartsch, K Quint
A diode-pump Nd:YAG high-power laser (wavelength 1320 nm, power 100 W) is routinely used to surgically remove lung metastases. Even pulmonary lesions in central locations are resectable via this method, yet it also carries a potential risk of damaging the larger bronchi and vessels in the vicinity. Studies investigating the safety of using high-power lasers are lacking. We therefore aimed to examine the direct effects of a 100-watt laser on the bronchi and pulmonary artery at a standard working velocity. From freshly slaughtered pigs, we isolated cylindrical specimens of the trachea, the main and lobar bronchi, and the central pulmonary artery from the both lungs...
March 14, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28293221/fish-as-hosts-of-vibrio-cholerae
#15
REVIEW
Malka Halpern, Ido Izhaki
Vibrio cholerae, the causative agent of pandemic cholera, is abundant in marine and freshwater environments. Copepods and chironomids are natural reservoirs of this species. However, the ways V. cholerae is globally disseminated are as yet unknown. Here we review the scientific literature that provides evidence for the possibility that some fish species may be reservoirs and vectors of V. cholerae. So far, V. cholerae has been isolated from 30 fish species (22 freshwater; 9 marine). V. cholerae O1 was reported in a few cases...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28290186/phosphorus-and-other-aspects-of-ckd-mbd-in-the-conservative-management-of-chronic-kidney-disease
#16
Anuja Shah
As the prevalence of chronic kidney disease (CKD) increases and the population ages, there is an imperative to offer cost effective and patient specific therapeutic options for the management of advanced CKD. In cases where there is a desire to avoid or delay renal replacement therapy, conservative options need to be defined and strategies for delaying the need for renal replacement therapy should be offered. CKD-mineral bone disorders (MBD) refers to the constellation of disturbances in abnormal bone and soft tissue calcification along with abnormalities, in phosphorus, calcium, parathyroid hormone, vitamin D, and FGF-23...
June 2017: Panminerva Medica
https://www.readbyqxmd.com/read/28280690/optimization-and-validation-of-cryostat-temperature-conditions-for-trans-reflectance-mode-ftir-microspectroscopic-imaging-of-biological-tissues
#17
Sumedha Liyanage, Rohan S Dassanayake, Amal Bouyanfif, Erandathi Rajakaruna, Latha Ramalingam, Naima Moustaid-Moussa, Noureddine Abidi
In Fourier transform infrared (FTIR) microspectrocopy, the tissue preparation method is crucial, especially how the tissue is cryo-sectioned prior to the imaging requires special consideration. Having a temperature difference between the cutting blade and the specimen holder of the cryostat greatly affects the quality of the sections. Therefore, we have developed an optimal protocol for cryo-sectioning of biological tissues by varying the temperature of both the cutting blade and the specimen holder. Using this protocol, we successfully cryo-sectioned four different difficult-to-section tissues including white adipose tissue (WAT), brown adipose tissue (BAT), lung, and liver...
2017: MethodsX
https://www.readbyqxmd.com/read/28276021/assessing-mitochondrial-bioenergetics-in-isolated-mitochondria-from-various-mouse-tissues-using-seahorse-xf96-analyzer
#18
Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, Holger Prokisch
Working with isolated mitochondria is the gold standard approach to investigate the function of the electron transport chain in tissues, free from the influence of other cellular factors. In this chapter, we outline a detailed protocol to measure the rate of oxygen consumption (OCR) with the high-throughput analyzer Seahorse XF96. More importantly, this protocol wants to provide practical tips for handling many different samples at once, and take a real advantage of using a high-throughput system. As a proof of concept, we have isolated mitochondria from brain, heart, liver, muscle, kidney, and lung of a wild-type mouse, and measured basal respiration (State II), ADP-stimulated respiration (State III), non-ADP-stimulated respiration (State IVo), and FCCP-stimulated respiration (State IIIu) using respiratory substrates specific to the respiratory chain complex I (RCCI) and complex II (RCCII)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28275764/a-novel-botryoidal-aramid-fiber-reinforcement-of-a-pmma-resin-for-a-restorative-biomaterial
#19
Xinye He, Ying Qu, Jinrong Peng, Tao Peng, Zhiyong Qian
Poly(methyl methacrylate) (PMMA) resin is widely used as a prosthetic and restorative biomaterial, such as in bone cement, denture base resin, etc. The flexural and compressive strength of a PMMA resin is of great concern and many approaches have been made to improve the flexural resistance and compressive strength of PMMA. To strengthen PMMA via high-performance (HP) fibers is a feasible way; however, the HP fibers are not very satisfactory in practice, with a complex handling process and esthetic concerns...
March 9, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28274872/robot-assisted-laparoscopic-transplantation-of-frozen-thawed-ovarian-tissue
#20
Kutluk Oktay, Enes Taylan, Yodo Sugishita, Gabriela M Goldberg
STUDY OBJECTIVE: To demonstrate a new approach for orthotopic human ovarian tissue transplantation via robot-assisted laparoscopic surgery. DESIGN: A step-by-step video explanation of the surgical technique (Canadian Task Force Classification-III). SETTING: Although still experimental, cryopreservation and subsequent transplantation of frozen-thawed ovarian tissue is currently the only available method for prepubertal girls and young women with cancer who are not eligible for established fertility preservation options such as the oocyte or embryo cryopreservation (1)...
March 5, 2017: Journal of Minimally Invasive Gynecology
keyword
keyword
110726
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"