keyword
MENU ▼
Read by QxMD icon Read
search

Tissue handling

keyword
https://www.readbyqxmd.com/read/28211878/pre-descemets-endothelial-keratoplasty-the-pdek-clamp-for-successful-pdek
#1
H S Dua, D G Said
PurposeTo design and produce a clamp that enables good handling of donor sclera-corneal disks, allows air to be injected in the corneal stroma and consistently provides pre-Descemets endothelial keratoplasty (PDEK) tissue by creation of a type-1 big bubble (BB) avoiding a type-2BB by shutting the fenestrations in the periphery of the pre-Descemets layer and preventing escape of air.Patients and methodsA clamp with spring loaded handles attached to two rings of 9 mm diameter and 1 mm width with a side port for insertion of a needle for air injection was designed and produced...
February 17, 2017: Eye
https://www.readbyqxmd.com/read/28210549/evaluation-of-discrepancies-in-weights-of-fresh-and-fixed-specimens-in-breast-surgery-a-retrospective-cohort-study
#2
Dinesh Thekkinkattil
BACKGROUND: Handling of breast specimen is not well monitored and there is limited data on the impact of fixative solutions on specimen weights. Weight of resected breast tissue acts as a guide for future reconstructive and symmetrisation procedures. The aim is to quantitatively evaluate the discrepancies in weight of fresh breast specimen and the weight of specimen after being kept in a fixative solution for a variable length of time. METHODS: Single centre retrospective cohort study including patients undergone breast surgery for both diagnostic and therapeutic purposes between January of 2013 and June 2015...
February 2017: Gland Surgery
https://www.readbyqxmd.com/read/28207828/the-wide-spectrum-high-biocidal-potency-of-bioxy-formulation-when-dissolved-in-water-at-different-concentrations
#3
Dori Dagher, Ken Ungar, Richard Robison, Fadi Dagher
Traditional surface disinfectants that have long been applied in medicine, animal husbandry, manufacturing and institutions are inconvenient at best and dangerous at worst. Moreover, some of these substances have adverse environmental impacts: for example, quaternary ammonium compounds ("quats") are reproductive toxicants in both fish and mammals. Halogens are corrosive both to metals and living tissues, are highly reactive, can be readily neutralized by metals, and react with organic matter to form toxic, persistent by-products such as dioxins and furans...
2017: PloS One
https://www.readbyqxmd.com/read/28198734/new-frontiers-in-intravital-microscopy-of-the-kidney
#4
Andrew M Hall, Claus D Schuh, Dominik Haenni
PURPOSE OF REVIEW: Intravital imaging with multiphoton microscopy enables the detailed study of dynamic cellular processes within functioning organs in living animals, in ways that would not otherwise be possible. It therefore represents a powerful tool in translational kidney research. In this article, we will discuss several new technical developments that have significantly increased the capabilities of kidney imaging. RECENT FINDINGS: Important contemporary advances in biomedical imaging technology include longer wavelength excitation lasers, far-red emitting fluorescent reporters, highly sensitive detectors, fluorescence lifetime measurements, adaptive optics, microendoscopes, high-throughput automated analysis algorithms and tissue clearing techniques...
February 13, 2017: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/28195244/health-technology-assessment-introducing-a-vacuum-based-preservation-system-for-biological-materials-in-the-anatomic-pathology-workflow
#5
R Saliceti, E Nicodemo, A Giannini, A Cortese
INTRODUCTION: The objective of this work is to assess the implementation of a newly introduced medical equipment technology for the vacuum-based preservation of biological materials within an Anatomic Pathology service. METHODS: The approach selected for the analysis is the Health Technology Assessment (HTA ), a comprehensive evaluation method based on relevant scientific evidence and designed to support healthcare decision makers in purchasing, replacing or disposing of technologies...
March 2016: Pathologica
https://www.readbyqxmd.com/read/28192624/mr-based-synthetic-ct-generation-using-a-deep-convolutional-neural-network-method
#6
Xiao Han
PURPOSE: Interests have been rapidly growing in the field of radiotherapy to replace CT with magnetic resonance imaging (MRI), due to superior soft tissue contrast offered by MRI and the desire to reduce unnecessary radiation dose. MR-only radiotherapy also simplifies clinical workflow and avoids uncertainties in aligning MR with CT. Methods, however, are needed to derive CT-equivalent representations, often known as synthetic CT (sCT), from patient MR images for the purpose of dose calculation and DRR-based patient positioning...
February 13, 2017: Medical Physics
https://www.readbyqxmd.com/read/28188518/a-robust-protocol-for-protein-extraction-and-digestion
#7
Michelle Atallah, Mark R Flory, Parag Mallick
Proteins play a key role in all aspects of cellular homeostasis. Proteomics, the large-scale study of proteins, provides in-depth data on protein properties, including abundances and post-translational modification states, and as such provides a rich avenue for the investigation of biological and disease processes. While proteomic tools such as mass spectrometry have enabled exquisitely sensitive sample analysis, sample preparation remains a critical unstandardized variable that can have a significant impact on downstream data readouts...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28187887/collapsed-cone-dose-calculations-for-heterogeneous-tissues-in-brachytherapy-using-primary-and-scatter-separation-source-data
#8
Anders Ahnesjö, Bob van Veelen, Åsa Carlsson Tedgren
BACKGROUND AND OBJECTIVE: Brachytherapy is a form of radiation therapy using sealed radiation sources inserted within or in the vicinity of the tumor of, e.g., gynecological, prostate or head- and neck cancers. Accurate dose calculation is a crucial part of the treatment planning. Several reviews have called for clinical software with model-based algorithms that better take into account the effects of patient individual distribution of tissues, source-channel and shielding attenuation than the commonly employed TG-43 formalism which simply map homogeneous water dose distributions onto the patient...
February 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28181097/the-efficacy-of-a-novel-tissue-grasper-clips-technique-for-large-perforations-of-the-sigmoid-colon-in-an-experimental-animal-model-video
#9
Jun Young Eun, Yunho Jung, Tae Hoon Lee, Young Sin Cho, Ho Sung Rhee, Young Kyu Jung, Joung-Ho Han, Duk Su Kim, Il Kwun Chung, Sang-Heum Park, Sun Joo Kim
BACKGROUND: The incidence of iatrogenic colonic perforation has been gradually increasing. In particular, sigmoid colon perforations are difficult to handle because of excess mobility. AIM: The aim of this study was to evaluate the efficacy of the twin grasper-clips technique for large perforations of the sigmoid colon. METHODS: This study was designed as a prospective, randomized, experimental study using ex vivo porcine colorectal specimens...
February 8, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28176856/characterization-of-adipogenic-chemicals-in-three-different-cell-culture-systems-implications-for-reproducibility-based-on-cell-source-and-handling
#10
Christopher D Kassotis, Lauren Masse, Stephanie Kim, Jennifer J Schlezinger, Thomas F Webster, Heather M Stapleton
The potential for chemical exposures to exacerbate the development and/or prevalence of metabolic disorders, such as obesity, is currently of great societal concern. Various in vitro assays are available to assess adipocyte differentiation, though little work has been done to standardize protocols and compare models effectively. This study compares several adipogenic cell culture systems under a variety of conditions to assess variability in responses. Two sources of 3T3-L1 preadipocytes as well as OP9 preadipocytes were assessed for cell proliferation and triglyceride accumulation following different induction periods and using various tissue culture plates...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28174120/xenobiotics-and-the-glucocorticoid-receptor
#11
REVIEW
Linda S M Gulliver
Glucocorticoid Receptor (GR) is present in virtually every human cell type. Representing a nuclear receptor superfamily, GR has several different isoforms essentially acting as ligand-dependent transcription factors, regulating glucocorticoid-responsive gene expression in both a positive and a negative manner. Although the natural ligand of the Glucocorticoid Receptor, glucocorticoids (GC) represent only some of the multiple ligands for GR. Xenobiotics, ubiquitous in the environment, bind to GR and are also capable of activating or repressing GR gene expression, thereby modulating GR cell and tissue-specific downstream effects in a multitude of ways that include responses to inflammatory, allergic, metabolic, neoplastic and autoimmune processes...
February 4, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28170157/combining-electrospinning-and-cell-sheet-technology-for-the-development-of-a-multiscale-tissue-engineered-ligament-construct-telc
#12
Cedryck Vaquette, P T Sudheesh Kumar, Eugen Bogdan Petcu, Saso Ivanovski
Ligament tissue rupture is a common sport injury. Although current treatment modalities can achieve appropriate reconstruction of the damaged ligament, they present significant drawbacks, mostly related to reduced tissue availability and pain associated with tissue harvesting. Stem cell based tissue regeneration combined with electrospun scaffolds represents a novel treatment method for torn ligaments. In this study, a low fiber density polycaprolactone (PCL) electrospun mesh and sheep mesenchymal stem cells (sMSCs) were used to develop tissue engineered ligament construct (TELC) in vitro...
February 7, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28168362/practical-methods-for-handling-human-periodontal-ligament-stem-cells-in-serum-free-and-serum-containing-culture-conditions-under-hypoxia-implications-for-regenerative-medicine
#13
Dai Murabayashi, Mai Mochizuki, Yuichi Tamaki, Taka Nakahara
Stem cell-based therapies depend on the reliable expansion of patient-derived mesenchymal stem cells (MSCs) in vitro. The supplementation of cell culture media with serum is associated with several risks; accordingly, serum-free media are commercially available for cell culture. Furthermore, hypoxia is known to accelerate the expansion of MSCs. The present study aimed to characterize the properties of periodontal ligament-derived MSCs (PDLSCs) cultivated in serum-free and serum-containing media, under hypoxic and normoxic conditions...
February 6, 2017: Human Cell
https://www.readbyqxmd.com/read/28168224/lifestyle-and-horizontal-gene-transfer-mediated-evolution-of-mucispirillum-schaedleri-a-core-member-of-the-murine-gut-microbiota
#14
Alexander Loy, Carina Pfann, Michaela Steinberger, Buck Hanson, Simone Herp, Sandrine Brugiroux, João Carlos Gomes Neto, Mark V Boekschoten, Clarissa Schwab, Tim Urich, Amanda E Ramer-Tait, Thomas Rattei, Bärbel Stecher, David Berry
Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides...
January 2017: MSystems
https://www.readbyqxmd.com/read/28166904/difference-between-male-and-female-workers-lifting-the-same-relative-load-when-palletizing-boxes
#15
A Plamondon, C Larivière, D Denis, H Mecheri, I Nastasia
A few biomechanical studies have contrasted the work techniques of female and male workers during manual material handling (MMH). A recent study showed that female workers differed from males mostly in the strategy they used to lift 15-kg boxes from the ground, especially regarding task duration, knee and back postures and interjoint coordination. However, the lifting technique difference observed in females compared to males was perhaps due to a strength differences. The objective of this study was to test whether female workers would repeat the same lifting technique with a load adjusted to their overall strength (females: 10 kg; males: 15 kg), which can be considered a "relative load" since the overall back strength of females is 2/3 that of males...
April 2017: Applied Ergonomics
https://www.readbyqxmd.com/read/28163571/prevalence-patterns-and-correlates-of-equestrian-injuries-in-malaysia-a-cross-sectional-study
#16
Nizar A Majeedkutty, Nor A B Khairulanuar
BACKGROUND: Equestrian sport carries with it an implicit risk of injury. Despite the frequency of injuries in equestrian sports, there is no published study on injuries of equestrian athletes in Malaysia. OBJECTIVE: The objective of this study was to determine the prevalence of injuries and its correlates among horseback riders. SUBJECTS AND METHODS: A web-based standardized questionnaire was used to collect data for this cross-sectional survey...
January 2017: Journal of Family & Community Medicine
https://www.readbyqxmd.com/read/28155637/cmip-a-software-package-capable-of-reconstructing-genome-wide-regulatory-networks-using-gene-expression-data
#17
Guangyong Zheng, Yaochen Xu, Xiujun Zhang, Zhi-Ping Liu, Zhuo Wang, Luonan Chen, Xin-Guang Zhu
BACKGROUND: A gene regulatory network (GRN) represents interactions of genes inside a cell or tissue, in which vertexes and edges stand for genes and their regulatory interactions respectively. Reconstruction of gene regulatory networks, in particular, genome-scale networks, is essential for comparative exploration of different species and mechanistic investigation of biological processes. Currently, most of network inference methods are computationally intensive, which are usually effective for small-scale tasks (e...
December 23, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/28154929/first-intraoperative-experience-with-three-dimensional-3d-high-definition-hd-nasal-endoscopy-for-lacrimal-surgeries
#18
Mohammad Javed Ali, Milind N Naik
The aim of this study is to report our preliminary experiences with regard to safety and feasibility of three-dimensional (3D) endoscopic lacrimal surgeries with a recently launched latest generation 3D endoscope. A 4-mm rigid three-dimensional (3D) endoscope (TIPCAM 1S 3D ORL(R), Karl Storz, Tuttlingen, Germany) was used. Fifteen patients who underwent various endoscopic lacrimal procedures by a single surgeon (MJA) were included. The procedures included probing with nasolacrimal intubation, cruciate marsupialization of intranasal cysts for congenital dacryoceles, powered endoscopic dacryocystorhinostomy, post-operative stent removal with ostium granuloma excision...
February 2, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28143503/genetic-polymorphisms-in-key-hypoxia-regulated-downstream-molecules-and-phenotypic-correlation-in-prostate-cancer
#19
Avelino Fraga, Ricardo Ribeiro, André Coelho, José Ramon Vizcaíno, Helena Coutinho, José Manuel Lopes, Paulo Príncipe, Carlos Lobato, Carlos Lopes, Rui Medeiros
BACKGROUND: In this study we sought if, in their quest to handle hypoxia, prostate tumors express target hypoxia-associated molecules and their correlation with putative functional genetic polymorphisms. METHODS: Representative areas of prostate carcinoma (n = 51) and of nodular prostate hyperplasia (n = 20) were analysed for hypoxia-inducible factor 1 alpha (HIF-1α), carbonic anhydrase IX (CAIX), lysyl oxidase (LOX) and vascular endothelial growth factor (VEGFR2) immunohistochemistry expression using a tissue microarray...
January 31, 2017: BMC Urology
https://www.readbyqxmd.com/read/28141967/telomerase-mechanism-of-telomere-synthesis
#20
R Alex Wu, Heather E Upton, Jacob M Vogan, Kathleen Collins
Telomerase is the essential reverse transcriptase required for linear chromosome maintenance in most eukaryotes. Telomerase supplements the tandem array of simple-sequence repeats at chromosome ends to compensate for the DNA erosion inherent in genome replication. The template for telomerase reverse transcriptase is within the RNA subunit of the ribonucleoprotein complex, which in cells contains additional telomerase holoenzyme proteins that assemble the active ribonucleoprotein and promote its function at telomeres...
January 30, 2017: Annual Review of Biochemistry
keyword
keyword
110726
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"