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Tissue Procurement

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https://www.readbyqxmd.com/read/28551055/fresh-osteochondral-allografts-procurement-and-tissue-donation-in-europe
#1
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
Fresh osteochondral allografts are a well-established treatment for large, full-thickness cartilage defects. The clinical outcome for carefully selected patients is very favorable, especially for the young and active and graft survival up to 25 years has been described in the literature. Furthermore, a high patient satisfaction rate has been reported, but the biggest obstacle to overcome is the availability of tissue for transplantation. Large fresh bone allografts for cartilage damage repair only can be harvested from organ donors following organ removal or cadaveric donors, preferably in the setting of an operation room to minimize possible contamination of the tissue...
May 11, 2017: Injury
https://www.readbyqxmd.com/read/28536798/pollutants-induced-oxidative-stress-dna-damage-and-cellular-deformities-in-clarias-gariepinus-burchell-from-river-yamuna-in-delhi-region-india
#2
Anil K Tyor, Kanika Pahwa
The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR's and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0...
May 23, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28510634/autologous-adipose-derived-tissue-matrix-part-i-biologic-characteristics
#3
Stephen A Schendel
Background: Autologous collagen is an ideal soft tissue filler and may serve as a matrix for stem cell implantation and growth. Procurement of autologous collagen has been limited, though, secondary to a sufficient source. Liposuction is a widely performed and could be a source of autologous collagen. Objectives: The amount of collagen and its composition in liposuctioned fat remains unknown. The purpose of this research was to characterize an adipose-derived tissue-based product created using ultrasonic cavitation and cryo-grinding...
May 16, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28509699/penis-transplantation-first-us-experience
#4
Curtis L Cetrulo, Kai Li, Harry M Salinas, Matthew D Treiser, Ilse Schol, Glen W Barrisford, Francis J McGovern, Adam S Feldman, Michael T Grant, Cigdem Tanrikut, Jeffrey H Lee, Richard J Ehrlichman, Paul W Holzer, Garry M Choy, Raymond W Liu, Zhi Yang Ng, Alexandre G Lellouch, Josef M Kurtz, William G Austen, Jonathan M Winograd, Branko Bojovic, Kyle R Eberlin, Ivy A Rosales, Robert B Colvin, Dicken S C Ko
OBJECTIVE: We describe the first successful penis transplant in the United States in a patient with a history of subtotal penectomy for penile cancer. BACKGROUND: Penis transplantation represents a new paradigm in restoring anatomic appearance, urine conduit, and sexual function after genitourinary tissue loss. To date, only 2 penis transplants have been performed worldwide. METHODS: After institutional review board approval, extensive medical, surgical, and radiological evaluations of the patient were performed...
May 15, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28501997/using-laser-capture-microdissection-to-isolate-cortical-laminae-in-nonhuman-primate-brain
#5
Brian A Corgiat, Claudius Mueller
Laser capture microdissection (LCM) is a technique that allows procurement of an enriched cell population from a heterogeneous tissue sample under direct microscopic visualization. Fundamentally, laser capture microdissection consists of three main steps: (1) visualizing the desired cell population by microscopy, (2) melting a thermolabile polymer onto the desired cell populations using infrared laser energy to form a polymer-cell composite (capture method) or photovolatizing a region of tissue using ultraviolet laser energy (cutting method), and (3) removing the desired cell population from the heterogeneous tissue...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28499885/the-role-of-gilz-in-modulation-of-adaptive-immunity-in-a-murine-model-of-myocardial-infarction
#6
Babak Baban, Lin Yin, Xu Qin, Jun Yao Liu, Xingming Shi, Mahmood S Mozaffari
Myocardial infarction (MI) is associated with intense immune and inflammatory responses which contribute to tissue injury. Increasing evidence indicates that the glucocorticoid-induced leucine zipper (GILZ) protein suppresses immune and inflammatory responses. However, the status of and the role of GILZ in MI are not known. We tested the hypotheses that a) MI reduces cardiac GILZ associated with intense inflammation and cell death and b) intramyocardial GILZ delivery confers cardioprotection in association with increased Tregs and suppression of inflammation...
May 10, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28497127/evidence-of-glycolysis-up-regulation-and-pyruvate-mitochondrial-oxidation-mismatch-during-mechanical-unloading-of-the-failing-human-heart-implications-for-cardiac-reloading-and-conditioning
#7
Nikolaos A Diakos, Sutip Navankasattusas, E Dale Abel, Jared Rutter, Lauren McCreath, Peter Ferrin, Stephen H McKellar, Dylan V Miller, Song Y Park, Russell S Richardson, Ralph Deberardinis, James E Cox, Abdallah G Kfoury, Craig H Selzman, Josef Stehlik, James C Fang, Dean Y Li, Stavros G Drakos
This study sought to investigate the effects of mechanical unloading on myocardial energetics and the metabolic perturbation of heart failure (HF) in an effort to identify potential new therapeutic targets that could enhance the unloading-induced cardiac recovery. The authors prospectively examined paired human myocardial tissue procured from 31 advanced HF patients at left ventricular assist device (LVAD) implant and at heart transplant plus tissue from 11 normal donors. They identified increased post-LVAD glycolytic metabolites without a coordinate increase in early, tricarboxylic acid (TCA) cycle intermediates...
October 2016: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28497108/a-meta-analysis-of-endoscopic-ultrasound-fine-needle-aspiration-compared-to-endoscopic-ultrasound-fine-needle-biopsy-diagnostic-yield-and-the-value-of-onsite-cytopathological-assessment
#8
REVIEW
Muhammad Ali Khan, Ian S Grimm, Bilal Ali, Richard Nollan, Claudio Tombazzi, Mohammad Kashif Ismail, Todd H Baron
Background The diagnostic yield of endoscopic ultrasound (EUS) guided fine-needle aspiration (FNA) is variable, and partly dependent upon rapid onsite evaluation (ROSE) by a cytopathologist. Second generation fine-needle biopsy (FNB) needles are being increasingly used to obtain core histological tissue samples. Aims Studies comparing the diagnostic yield of EUS guided FNA versus FNB have reached conflicting conclusions. We therefore conducted a systematic review and meta-analysis to compare the diagnostic yield of FNA with FNB, and specifically evaluating the diagnostic value of ROSE while comparing the two types of needles...
May 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28495542/endothelial-function-is-preserved-in-veins-harvested-by-either-endoscopic-or-surgical-techniques
#9
Adam R Wheeler, Daniel E Kendrick, Matt T Allemang, Andre F Gosling, Ann H Kim, Alfred Hausladen, Vikram S Kashyap
OBJECTIVES: Endoscopic vein harvest for lower extremity arterial bypass grafting has been questioned due to concern for endothelial damage during procurement. We sought to compare Nitric Oxide (NO)-mediated endothelial dependent relaxation (EDR) in vein segments harvested using open surgical (OH) versus endoscopic (EH) techniques. METHODS: Saphenous vein segments were harvested for lower extremity bypass, and a single, minimally handled section of saphenous vein, free of branches, was taken from the end of the graft...
May 8, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28493355/consideration-of-difficulties-and-exit-strategies-in-a-case-of-face-allotransplantation-resulting-in-failure
#10
Özlenen Özkan, Ömer Özkan, Umuttan Doğan, Vural Taner Yılmaz, Hilmi Uysal, Levent Ündar, Ebru Apaydın Doğan, Ozan Salim, Anı Çinpolat, Atilla Ramazanoglu
We describe the first rescue procedure in a case of total face allotransplantation. The recipient was a 54-year-old man with severe disfigurement of the entire face following an accidental gunshot injury 5 years previously. The large defect included the maxilla, mandible, and mid-face. Full face procurement was performed from a multiorgan cadaveric donor and was allotransplanted to the recipient. The post-transplant induction immunosuppressive regimen included ATG combined with tacrolimus, mycophenolate mofetil, and prednisone, while maintenance was provided by the last three of these...
May 11, 2017: Microsurgery
https://www.readbyqxmd.com/read/28490924/improving-molecular-testing-and-personalized-medicine-in-non-small-cell-lung-cancer-in-ontario
#11
C Lim, H S Sekhon, J C Cutz, D M Hwang, S Kamel-Reid, R F Carter, G da Cunha Santos, T Waddell, M Binnie, M Patel, N Paul, T Chung, A Brade, R El-Maraghi, C Sit, M S Tsao, N B Leighl
BACKGROUND: Although molecular testing has become standard in managing advanced nonsquamous non-small-cell lung cancer (nsclc), most patients undergo minimally invasive procedures, and the diagnostic tumour specimens available for testing are usually limited. A knowledge translation initiative to educate diagnostic specialists about sampling techniques and laboratory processes was undertaken to improve the uptake and application of molecular testing in advanced lung cancer. METHODS: A multidisciplinary panel of physician experts including pathologists, respirologists, interventional thoracic radiologists, thoracic surgeons, medical oncologists, and radiation oncologists developed a specialty-specific education program, adapting international clinical guidelines to the local Ontario context...
April 2017: Current Oncology
https://www.readbyqxmd.com/read/28483262/lungs-donated-after-circulatory-death-and-prolonged-warm-ischemia-are-transplanted-successfully-after-enhanced-ex%C3%A2-vivo-lung-perfusion-using-adenosine-a2b-receptor-antagonism
#12
Eric J Charles, J Hunter Mehaffey, Ashish K Sharma, Yunge Zhao, Mark H Stoler, James M Isbell, Christine L Lau, Curtis G Tribble, Victor E Laubach, Irving L Kron
OBJECTIVE: The current supply of acceptable donor lungs is not sufficient for the number of patients awaiting transplantation. We hypothesized that ex vivo lung perfusion (EVLP) with targeted drug therapy would allow successful rehabilitation and transplantation of donation after circulatory death lungs exposed to 2 hours of warm ischemia. METHODS: Donor porcine lungs were procured after 2 hours of warm ischemia postcardiac arrest and subjected to 4 hours of cold preservation or EVLP...
April 12, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28476699/analysis-and-detection-of-microporosities-in-aging-bones-in-context-to-pharmaceutical-treatment-a-study-in-line-of-osteoporosis
#13
Nadeem Baig, Muhammad Zaheer Khan
The objective of the current study was to evaluate the effects of nandrolone decanoate in terms of morphological changes in the diaphyseal region of appendicular skeleton long bone (femur) of aging female Albino rats with the help of innovatively designed histomorphometric tool of study being introduced first time mapped out micro porosities (lacunae) which tend to intensify as part of advanced aging denoting deterioration in osseous tissue. In fact our research trial has been in the line of involutional osteoporosis especially common in post-menopausal aged women, as to find out appropriate drug usefulness in the care and cure of this diseased state as per recommendation of FDA...
September 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28454715/a-pcr-based-method-for-quantifying-neutrophils-in-human-nasal-secretions
#14
Matthew C Morris, M Nadeem Khan, Michael E Pichichero
Neutrophil recruitment to the nasopharynx (NP) is a central event in resolution of NP-initiated microbial infections. A vigorous neutrophil response in infected tissues is also associated with the outcome of adverse tissue pathology. Therefore, differences in infection-induced tissue neutrophil numbers may correlate with pathogenesis events. Existing methods of quantifying neutrophils require evaluation of NP samples within hours of procurement as flow cytometry based cell quantification methods require live neutrophil cells...
April 25, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28444910/characterization-of-porcine-endogenous-retrovirus-expression-in-neonatal-and-adult-pig-pancreatic-islets
#15
Nizar I Mourad, Claire Crossan, Victoria Cruikshank, Linda Scobie, Pierre Gianello
BACKGROUND: Pig islets represent an alternative to the current modes of treatment for patients with diabetes. However, the concerns over pathogen transmission including that of PERV limit their immediate, widespread usage in humans. It has been previously demonstrated that PERV copy number and particularly expression levels can vary considerably between individuals and within different tissues of a single animal. In general, expression levels have been found to be particularly low in the pancreas compared to other porcine tissues suggesting a reduced risk associated with the use of this tissue...
April 26, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28439732/skin-allograft-procurement-and-transplantation-in-mashhad-iran-are-burn-patients-needs-being-met
#16
Seyed Hassan Tavousi, Ali Ahmadabadi, Alireza Sedaghat, Ebrahim Khaleghi, Maryam Rashchi, Zohreh Bonakdaran
Only a single donor's tissue may save or improve lives of one hundred patients. Unfortunately, low governmental and media support of tissue procurement and transplantation programs is a worldwide problem. Loss of an effective tissue procurement program in many countries like Iran, may lead to loss of many thousands valuable tissues each year. To evaluate the rate of skin donation in Mashhad in comparison to other organs and tissues, we extracted the data related to tissue and organ procurement in Mashhad from 2001...
April 24, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28437367/-effective-requesting-a-scoping-review-of-the-literature-on-asking-families-to-consent-to-organ-and-tissue-donation
#17
Jennifer A Chandler, Matthew Connors, Giles Holland, Sam D Shemie
BACKGROUND: Families are often asked to consent to the donation of their deceased relative's organs or tissues. These end-of-life conversations are important because they affect consent rates as well as the psychological impact of the decision for families. This scoping review of the literature on requesting family consent was prepared to support of the development of leading practice recommendations for end-of-life conversations with families of potential donors. A scoping review maps research activity in a field across a range of commentary and empirical study designs but does not attempt meta-synthesis of empirical data or quality assessment...
May 2017: Transplantation
https://www.readbyqxmd.com/read/28395880/treatment-of-metastatic-uveal-melanoma-with-adoptive-transfer-of-tumour-infiltrating-lymphocytes-a-single-centre-two-stage-single-arm-phase-2-study
#18
Smita S Chandran, Robert P T Somerville, James C Yang, Richard M Sherry, Christopher A Klebanoff, Stephanie L Goff, John R Wunderlich, David N Danforth, Daniel Zlott, Biman C Paria, Arvind C Sabesan, Abhishek K Srivastava, Liqiang Xi, Trinh H Pham, Mark Raffeld, Donald E White, Mary Ann Toomey, Steven A Rosenberg, Udai S Kammula
BACKGROUND: Uveal melanoma is a rare tumour with no established treatments once metastases develop. Although a variety of immune-based therapies have shown efficacy in metastatic cutaneous melanoma, their use in ocular variants has been disappointing. Recently, adoptive T-cell therapy has shown salvage responses in multiple refractory solid tumours. Thus, we sought to determine if adoptive transfer of autologous tumour-infiltrating lymphocytes (TILs) could mediate regression of metastatic uveal melanoma...
April 7, 2017: Lancet Oncology
https://www.readbyqxmd.com/read/28395322/de-and-recellularization-of-the-pig-uterus-a-bioengineering-pilot-study%C3%A2
#19
Hannes Campo, Pedro M Baptista, Nuria López-Pérez, Amparo Faus, Irene Cervelló, Carlos Simón
Absolute uterine factor infertility, or the absence of a functional uterus, has a prevalence of 3%-5% in the general population. Despite the great strides being made in reproductive medicine, patients diagnosed with absolute uterine factor infertility remain untreatable. The only available solution has been gestational surrogacy, but recently the Brannström group presented a viable alternative by reporting the first successful live birth after uterus transplantation. Similar to other transplantations, this approach has inherent limitations such as the paucity of donor organs and the need for long-term immunosuppression...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28373416/on-site-cytology-for-development-of-patient-derived-three-dimensional-organoid-cultures-a-pilot-study
#20
Verena Sailer, Chantal Pauli, Elyze C Merzier, Juan Miguel Mosquera, Himisha Beltran, Mark A Rubin, Rema A Rao
BACKGROUND/AIM: Development of patient-derived three-dimensional (3D) organoid cultures is an emerging technique in the field of precision oncology. We aimed to integrate on-site adequacy evaluation using cytology into the tumor organoid development workflow to ensure precise characterization and growth of these cultures. PATIENTS AND METHODS: Cancer patients were consented to a Precision Medicine trial. Fresh tissue was procured for genomic analyses as well as organoid development...
April 2017: Anticancer Research
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