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Tissue Procurement

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https://www.readbyqxmd.com/read/29344855/protocol-for-the-analysis-of-laser-capture-microdissected-fresh-frozen-tissue-homogenates-by-silver-stained-1d-sds-page
#1
DaRue A Prieto, Gordon Whitely, Donald J Johann, Josip Blonder
The heterogeneity present in solid tumors adds significant difficulty to scientific analysis and improved understanding. Fundamentally, solid tumor formation consists of cancer cells proper along with stromal elements. The burgeoning malignant process is dependent upon modified stromal elements. Collectively, the stroma forms an essential microenvironment, which is indispensable for the survival and growth of the malignant neoplasm. This cellular heterogeneity makes molecular profiling of solid tumors via mass spectrometry (MS)-based proteomics a daunting task...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344852/laser-capture-microdissection-insights-into-methods-and-applications
#2
Meera Mahalingam
Laser capture microdissection is a non-molecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. Its advantages include efficient rapid and precise procurement of cells. The potential disadvantages include time consuming, expensive, and limited by the need for a pathologist for recognition of distinct subpopulations within a specified sample. Overall it is versatile allowing the preparation of homogenous isolates of specific subpopulations of cells from which DNA/RNA or protein can be extracted for RT-PCR, quantitative PCR, next-generation sequencing, immunoblot blot analyses, and mass spectrometry...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29317046/retro-peritoneal-cooling-for-kidney-preservation-from-multi-organ-cadaver-donors
#3
Anastasio Salazar-Bañuelos, Mauricio Monroy-Cuadros, Hoover Henriquez-Cooper
BACKGROUND: Minimizing ischemia is paramount in the procurement of kidneys for transplantation. A fast cooling and expeditious removal is ideal to minimize damage from warm ischemia, however, since the removal of kidneys is delayed in cadaver donation until all other organs are harvested, the risk of kidney damage increases due to contact with the warmer soft body tissues. Surgical techniques that expedite organ retrieval were developed to avoid organ damage. METHODS: We test a modification of Thomas Starzl's improved technique for multi-organ harvesting by interposing an ice bag between the posterior aspect of the kidney and the psoas muscle in a randomized trial with 21 multi-organ cadaver donors...
January 4, 2018: American Journal of Surgery
https://www.readbyqxmd.com/read/29305893/randomized-trial-comparing-the-franseen-and-fork-tip-needles-for-eus-guided-fine-needle-biopsy-of-solid-pancreatic-mass-lesions
#4
Ji Young Bang, Shantel Hebert-Magee, Udayakumar Navaneethan, Muhammad K Hasan, Robert Hawes, Shyam Varadarajulu
BACKGROUND AND AIMS: Recently, 3-plane symmetric needle with Franseen geometry and Fork-tip biopsy needle have been developed for histological tissue procurement. We compared 22-gauge Franseen and 22-gauge Fork-tip needles in patients undergoing EUS-guided sampling of pancreatic masses. METHODS: Fifty patients underwent sampling using both 22-gauge Franseen and 22-gauge Fork-tip needles, with randomization of needle order. Two dedicated passes were performed using both needles for cell block...
January 3, 2018: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/29286394/processing-of-human-cardiac-tissue-toward-extracellular-matrix-self-assembling-hydrogel-for-in-vitro-and-in-vivo-applications
#5
Matthias Becker, Janita A Maring, Barbara Oberwallner, Benjamin Kappler, Oliver Klein, Volkmar Falk, Christof Stamm
Acellular extracellular matrix preparations are useful for studying cell-matrix interactions and facilitate regenerative cell therapy applications. Several commercial extracellular matrix products are available as hydrogels or membranes, but these do not possess tissue-specific biological activity. Because perfusion decellularization is usually not possible with human heart tissue, we developed a 3-step immersion decellularization process. Human myocardial slices procured during surgery are first treated with detergent-free hyperosmolar lysis buffer, followed by incubation with the ionic detergent, sodium dodecyl sulfate, and the process is completed by exploiting the intrinsic DNase activity of fetal bovine serum...
December 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29220079/hepatitis-c-virus-genotyping-of-organ-donor-samples-to-aid-in-transplantation-of-hcv-positive-organs
#6
Caren Gentile, Vivianna M Van Deerlin, David S Goldberg, Peter P Reese, Richard D Hasz, Peter Abt, Emily Blumberg, Midhat S Farooqi
Given the availability of new highly efficacious anti-HCV therapies, some clinicians have advocated for wider use of kidneys from hepatitis C virus-positive (HCV+) donors, including transplanting them into HCV-negative recipients. As treatment regimens for HCV are commonly guided by genotype, pretransplant HCV genotyping of tissue donors would be beneficial. To our knowledge, donor HCV genotyping has never been reported. We retrieved archived frozen plasma samples for 17 previous organ donors through a local organ procurement organization...
December 8, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/29204889/authorization-of-tissues-from-deceased-patients-for-genetic-research
#7
Maureen Wilson-Genderson, K Laura Barker, Heather M Gardiner, Maghboeba Mosavel, Jeffrey Thomas, Laura A Siminoff
Tissues from deceased donors provide important data for genomic research and Organ Procurement Organizations (OPOs) play a significant role. To understand the decisions of families who donated for transplantation and made decisions about donation to the Genotype-Tissue Expression Project (GTEx), we examined donation decisions of family decision makers (FDMs). 413 families were interviewed by telephone. The OPO staff who made the transplant and research requests completed self-administered surveys; a total of 309 matching surveys from 99 OPO staff were obtained...
December 4, 2017: Human Genetics
https://www.readbyqxmd.com/read/29198667/donation-after-circulatory-arrest-in-pancreas-transplantation-a-report-of-10-cases
#8
J A Fridell, R S Mangus, C M Thomas, C A Kubal, J A Powelson
INTRODUCTION: Transplantation of pancreas allografts procured from donation after circulatory death (DCD) remains uncommon. This study reviews a series of pancreas transplants at a single center to assess the donor and recipient characteristics for DCD pancreas transplant and to compare clinical outcomes. METHODS: DCD procurement was performed with a 5-minute wait time from pronouncement of death to first incision. In 2 patients, tissue plasminogen activator was infused as a thrombolytic during the donor flush...
December 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29194344/direct-peritoneal-resuscitation-alters-leukocyte-infiltration-in-the-lung-after-acute-brain-death
#9
Jessica L Weaver, Paul J Matheson, Amy Matheson, Cynthia D Downard, R Neal Garrison, Jason W Smith
BACKGROUND: Brain death is associated with significant lung injury and inflammation. This has been associated with worse long-term outcomes for transplanted lungs. Direct peritoneal resuscitation (DPR) reduces systemic inflammation in brain death and improves lung procurement rate. The effect of DPR on macrophage and neutrophil infiltration in the lungs is not known. METHODS: Male Sprague-Dawley rats had a 4F Fogarty catheter inserted into the skull and the balloon inflated until brain death was achieved...
November 30, 2017: Shock
https://www.readbyqxmd.com/read/29194322/direct-peritoneal-resuscitation-reduces-intestinal-permeability-after-brain-death
#10
Jessica L Weaver, Paul J Matheson, Amy Matheson, Brian G Harbrecht, Cynthia D Downard, R Neal Garrison, Jason W Smith
BACKGROUND: The profound inflammatory response associated with brain death is frequently cited as the reason organs procured from brain dead donors are associated with worse graft function. The intestine releases inflammatory mediators in other types of shock, but its role is brain death has not been well-studied. Direct peritoneal resuscitation (DPR) improves visceral organ blood flow and reduces inflammation after hemorrhagic shock. We hypothesized that use of DPR would maintain intestinal integrity and reduce circulating inflammatory mediators after brain death...
December 1, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29184753/tissue-augmentation-with-allograft-adipose-matrix-for-the-diabetic-foot-in-remission
#11
Tala B Shahin, Kairavi V Vaishnav, Marcy Watchman, Vignesh Subbian, Ethan Larson, Evangelia Chnari, David G Armstrong
Background: Repetitive stress on the neuropathic plantar foot is the primary cause of diabetic foot ulcers. After healing, recurrence is common. Modulating plantar pressure has been associated with extension of ulcer free days. Therefore, the goal of this study was to determine the effects of an injectable allograft adipose matrix in providing a protective padding and reducing the pressure in the plantar foot. Methods: After healing his recurrent ulcer using total contact casting, a 71-year-old man with a 9-year history of recurrent diabetic foot ulcers was treated with injection of allograft adipose matrix, procured from donated human tissue...
October 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29184457/evaluation-of-eu-legislation-on-blood-a-bioethical-point-of-view
#12
EDITORIAL
Carlo Petrini
A review of the European Union (EU) regulations concerning blood, tissues, and cells of human origin is under way in the EU. From the ethical point of view, the non-remuneration of donations and the ban on deriving gain from human biological materials are of particular significance. While the basic ethical principles involved in the procurement, preservation, and use of these materials are the same, their practical application should be adapted to the specific context of each material. In the case of donation and use of blood, in particular, the issue of legitimate reimbursements to donors and for transfusion centers has to be managed in accordance with the principle of non-commercialization...
2017: Journal of Blood Medicine
https://www.readbyqxmd.com/read/29150791/16-channel-flexible-system-to-measure-electrophysiological-properties-of-bioengineered-hearts
#13
Betsy H Salazar, Kristopher A Hoffman, Anilkumar K Reddy, Sridhar Madala, Ravi K Birla
As tissue engineering continues to mature, it is necessary to develop new technologies that bring insight into current paradigms and guide improvements for future experiments. To this end, we have developed a system to characterize our bioartificial heart model and compare them to functional native structures. In the present study, the hearts of adult Sprague-Dawley were decellularized resulting in a natural three-dimensional cardiac scaffold. Neonatal rat primary cardiac cells were then cultured within a complex 3D fibrin gel, forming a 3-dimensional cardiac construct, which was sutured to the acellular scaffold and suspended in media for 24-48 h...
November 17, 2017: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/29140906/nose-and-lips-graft-variants-a-subunit-anatomical-study
#14
Jérôme Duisit, Louis Maistriaux, Alexander Gerdom, Martial Vergauwen, Pierre Gianello, Catherine Behets, Benoît Lengelé
BACKGROUND: In the field of Vascularized Composite tissue Allotransplantation (VCA), the surgical design of facial subunit grafts is an evolving concept. The purpose of the present paper is to study the possibilities to divide the historical nose and lips face transplant into several morphological and functional subunit grafts, depending on their respective supply. METHODS: Our study was conducted in 20 adult cadavers. The facial artery and its branches were dissected bilaterally in 16 fresh and 4 embalmed heads...
November 11, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29131071/highly-selective-endothelin-1-receptor-a-inhibition-prevents-bleomycin-induced-pulmonary-inflammation-and-fibrosis-in-mice
#15
Nikolaos Manitsopoulos, Ioanna Nikitopoulou, Nikolaos A Maniatis, Christina Magkou, Anastasia Kotanidou, Stylianos E Orfanos
BACKGROUND: Pulmonary fibrosis is a chronic disease, which progressively leads to respiratory failure and ultimately death. Endothelin-1 (ET-1), a vasoconstrictor secreted by endothelial cells, promotes vasoconstriction by activation of its receptors A and B. OBJECTIVES: We addressed the role of highly selective ET-1 receptor A (ETA) inhibition in the pathogenesis of experimental pulmonary fibrosis by bleomycin (BLM). METHODS: BLM sulfate (2 U/mL) or saline was intratracheally administered to C57/Bl6 mice (4 groups; n = 5-11/group)...
November 9, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/29125624/non-small-cell-lung-cancer-clinical-trials-requiring-biopsies-with-biomarker-specific-results-for-enrollment-provide-unique-challenges
#16
REVIEW
Marshall L Spiegel, Jonathan W Goldman, Brian R Wolf, Danielle J Nameth, Tristan R Grogan, Aaron E Lisberg, Deborah J L Wong, Blanca A Ledezma, Melody A Mendenhall, Scott J Genshaft, Antonio J Gutierrez, Fereidoun Abtin, W Dean Wallace, Carlos R Adame, Jordan R McKenzie, Phillip A Abarca, Alice J Li, Jennifer L Strunck, Sina Famenini, James M Carroll, D Andrew Tucker, Lauren M Sauer, Nima M Moghadam, David A Elashoff, Christina D Abaya, Meghan B Brennan, Edward B Garon
BACKGROUND: Clinical trials in lung cancer increasingly require patients to provide fresh tumor tissue as a prerequisite to enrollment. The effects of this requirement on enrollment rates, enrollment durations, and patient selection have not been fully elucidated. METHODS: The authors retrospectively reviewed data generated by patients who consented to 1 or more interventional lung cancer clinical trials at the University of California-Los Angeles Jonsson Comprehensive Cancer Center between January 2013 and December 2014...
December 15, 2017: Cancer
https://www.readbyqxmd.com/read/29116514/combine-phage-antibody-display-library-selection-on-patient-tissue-specimens-with-laser-capture-microdissection-to-identify-novel-human-antibodies-targeting-clinically-relevant-tumor-antigens
#17
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu
A functional approach to generate tumor-targeting human monoclonal antibodies is through selection of phage antibody display libraries directly on tumor cells. Although technically convenient, the use of cancer cell lines for the selection has limitations as those cell lines often undergo genetic and epigenetic changes during prolonged in vitro culture and alter their cell surface antigen expression profile. The key is to develop a technology that allows selection of phage antibody display libraries on tumor cells in situ residing in their natural tissue microenvironment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29075760/the-nine-step-minnesota-grading-system-for-eyebank-eyes-with-age-related-macular-degeneration-a-systematic-approach-to-study-disease-stages
#18
Timothy W Olsen, Albert Liao, Hershonna S Robinson, Neal V Palejwala, Nicholas Sprehe
Purpose: To refine the Minnesota Grading System (MGS) using definitions from the Age-Related Eye Disease Studies (AREDS) into a nine-step grading scale (MGS-9). Methods: A nine-step grading scale descriptive analysis using three key phenotypic features (total drusen area, increased, and decreased pigmentation) of human eyebank eyes that were graded according to definitions from the AREDS criteria in order to harmonize studies of disease progression for research involving human tissue...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29056576/development-of-a-patient-specific-tumor-mold-using-mri-and-3d-printing-technology-for-targeted-tissue-procurement-and-radiomics-analysis-of-renal-masses
#19
Durgesh Kumar Dwivedi, Yonatan Chatzinoff, Yue Zhang, Qing Yuan, Michael Fulkerson, Rajiv Chopra, James Brugarolas, Jeffrey A Cadeddu, Payal Kapur, Ivan Pedrosa
OBJECTIVE: To implement a platform for co-localization of in vivo quantitative multi-parametric magnetic resonance imaging (mpMRI) features with ex vivo surgical specimens of patients with renal masses using patient-specific 3D-printed tumor molds, which may aid in targeted tissue procurement and radiomics/radiogenomic analyses. METHODS: Volumetric segmentation of 6 renal masses was performed with 3D Slicer (http://www.slicer.org) to create a three-dimensional (3D) tumor model...
October 19, 2017: Urology
https://www.readbyqxmd.com/read/29052751/-prevention-of-infections-due-to-musculoskeletal-allografts
#20
REVIEW
A Pruß
Transplantation of musculoskeletal tissues is widely used in the treatment of extensive defects of the musculoskeletal system, especially in orthopedics for exchange of prostheses, surgical interventions on the spine and in traumatology for reconstruction after extensive tumor resections. A danger after transplantation is the potential transmission of clinically relevant pathogens. Tissue banks have therefore established a safety level approach for musculoskeletal tissue transplants, which includes donor selection, laboratory testing, tissue procurement, tissue processing, tissue storage and quality assurance...
November 2017: Der Unfallchirurg
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