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Tissue Procurement

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https://www.readbyqxmd.com/read/28395880/treatment-of-metastatic-uveal-melanoma-with-adoptive-transfer-of-tumour-infiltrating-lymphocytes-a-single-centre-two-stage-single-arm-phase-2-study
#1
Smita S Chandran, Robert P T Somerville, James C Yang, Richard M Sherry, Christopher A Klebanoff, Stephanie L Goff, John R Wunderlich, David N Danforth, Daniel Zlott, Biman C Paria, Arvind C Sabesan, Abhishek K Srivastava, Liqiang Xi, Trinh H Pham, Mark Raffeld, Donald E White, Mary Ann Toomey, Steven A Rosenberg, Udai S Kammula
BACKGROUND: Uveal melanoma is a rare tumour with no established treatments once metastases develop. Although a variety of immune-based therapies have shown efficacy in metastatic cutaneous melanoma, their use in ocular variants has been disappointing. Recently, adoptive T-cell therapy has shown salvage responses in multiple refractory solid tumours. Thus, we sought to determine if adoptive transfer of autologous tumour-infiltrating lymphocytes (TILs) could mediate regression of metastatic uveal melanoma...
April 7, 2017: Lancet Oncology
https://www.readbyqxmd.com/read/28395322/de-and-recellularization-of-the-pig-uterus-a-bioengineering-pilot-study%C3%A2
#2
Hannes Campo, Pedro M Baptista, Nuria López-Pérez, Amparo Faus, Irene Cervelló, Carlos Simón
Absolute uterine factor infertility, or the absence of a functional uterus, has a prevalence of 3%-5% in the general population. Despite the great strides being made in reproductive medicine, patients diagnosed with absolute uterine factor infertility remain untreatable. The only available solution has been gestational surrogacy, but recently the Brannström group presented a viable alternative by reporting the first successful live birth after uterus transplantation. Similar to other transplantations, this approach has inherent limitations such as the paucity of donor organs and the need for long-term immunosuppression...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28373416/on-site-cytology-for-development-of-patient-derived-three-dimensional-organoid-cultures-a-pilot-study
#3
Verena Sailer, Chantal Pauli, Elyze C Merzier, Juan Miguel Mosquera, Himisha Beltran, Mark A Rubin, Rema A Rao
BACKGROUND/AIM: Development of patient-derived three-dimensional (3D) organoid cultures is an emerging technique in the field of precision oncology. We aimed to integrate on-site adequacy evaluation using cytology into the tumor organoid development workflow to ensure precise characterization and growth of these cultures. PATIENTS AND METHODS: Cancer patients were consented to a Precision Medicine trial. Fresh tissue was procured for genomic analyses as well as organoid development...
April 2017: Anticancer Research
https://www.readbyqxmd.com/read/28369799/effect-of-dental-pulp-stem-cells-in-mptp-induced-old-aged-mice-model
#4
Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Christopher Simon, Quan Fu Gan, Vui King Vincent-Chong, Vasudevan Mani, Kesavanarayanan Krishnan Selvarajan, Vellayan Subramaniam, Sabri Musa, Noor Hayaty Abu Kasim
Parkinson's disease (PD) is a neurodegenerative disease caused by the loss of dopaminergic (DA-ergic) neurons in the substantia nigra (SN) and represent as a huge threat to the geriatric population. Cell-replacement therapies (CRTs) have been proposed as a promising strategy to slow down or replace neuronal loss. Among the widely available cell sources, dental pulp stem cells (DPSCs) portray as an attractive source primarily due to their neural crest origin, ease of tissue procurement and less ethical hurdles MATERIALS AND METHODS: We first demonstrated the in vitro differentiation ability of DPSCs towards DA-ergic-like cells before evaluating their neuro-protection/neuro-restoration capacities in MPTP-induced mice...
March 30, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28359668/the-anterolateral-ligament-has-similar-biomechanical-and-histologic-properties-to-the-inferior-glenohumeral-ligament
#5
Kristof Smeets, Josh Slane, Lennart Scheys, Ramses Forsyth, Steven Claes, Johan Bellemans
PURPOSE: To characterize the tensile and histologic properties of the anterolateral ligament (ALL), inferior glenohumeral ligament (IGHL), and knee capsule. METHODS: Standardized samples of the ALL (n = 19), anterolateral knee capsule (n = 15), and IGHL (n = 13) were isolated from fresh-frozen human cadavers for uniaxial tensile testing to failure. An additional 6 samples of the ALL, capsule, and IGHL were procured for histologic analysis and determination of elastin content...
March 27, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28353261/information-management
#6
Alberto Labarga, Izaskun Beloqui, Angel G Martin
The collection and storage of human tissue samples has been undertaken in medicine for centuries; however, biobanking has only recently become a dedicated activity. The technological developments that have allowed the procurement and long-term storage of viable human cells ex vivo, and to obtain relevant scientific information, including genetic information, provide tremendous possibilities for advancing biomedical research. At the same time, these possibilities have raised complex information management issues regarding samples, processing, donor information, traceability, and use of the sample...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28353260/acquisition-and-reception-of-primary-tissues-cells-or-other-biological-specimens
#7
Lyn E Healy
The use and banking of biological material for research or clinical application is a well-established practice. The material can be of human or non-human origin. The processes involved in this type of activity, from the sourcing to receipt of materials, require adherence to a set of best practice principles that assure the ethical and legal procurement, traceability, and quality of materials.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28342285/development-of-a-human-cadaver-model-for-training-in-laparoscopic-donor-nephrectomy
#8
Erica Sutton, Adrian Billeter, Devin Druen, Henry Roberts, Jonathan Rice, Hiram C Polk
BACKGROUND: The Organ Procurement Network recommends a surgeon record 15 cases as surgeon or assistant for laparoscopic donor nephrectomies (LDN) prior to independent practice. The literature suggests that the learning curve for improved perioperative and patient outcomes is closer to 35 cases. In this article, we describe our development of a model utilizing fresh tissue and objective, quantifiable endpoints to document surgical progress and efficiency in each of the major steps involved in LDN...
March 25, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28339553/de-and-recellularization-of-the-pig-uterus-a-bioengineering-pilot-study%C3%A2
#9
Hannes Campo, Pedro M Baptista, Nuria López-Pérez, Amparo Faus, Irene Cervelló, Carlos Simón
Absolute uterine factor infertility, or the absence of a functional uterus, has a prevalence of 3%-5% in the general population. Despite the great strides being made in reproductive medicine, patients diagnosed with absolute uterine factor infertility remain untreatable. The only available solution has been gestational surrogacy, but recently the Brannström group presented a viable alternative by reporting the first successful live birth after uterus transplantation. Similar to other transplantations, this approach has inherent limitations such as the paucity of donor organs and the need for long-term immunosuppression...
December 27, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/28334554/rapid-establishment-of-the-european-bank-for-induced-pluripotent-stem-cells-ebisc-the-hot-start-experience
#10
Paul A De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha Rawat, Isobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Jürgen Hescheler, Oliver Brüstle, Michael Peitz, Cornelia Thiele, Niels Geijsen, Bjørn Holst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K Gupta, Alexander J Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolén, Andreas Ebneth, Alfredo Cabrera-Socorro, Patrik Foerch, Martine Geraerts, Tina C Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annette Ringwald, Beate Kreisel, Aidan Courtney, Timothy E Allsopp
A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28298840/the-need-for-a-population-based-dose-optimization-study-for-recombinant-tissue-plasminogen-activator-in-acute-ischemic-stroke-a-study-from-a-tertiary-care-teaching-hospital-from-south-india
#11
Siju V Abraham, Fazil Thaha, S Vimal Krishnan, Athulya Shajan, Jayaraj Mymbilly Balakrishnan, Babu Urumese Palatty
CONTEXT: The guideline recommended dose of intravenous (i.v) recombinant tissue-type plasminogen activator (rt-PA) for acute ischemic stroke is 0.9 mg/kg in the European and American populations. In Asiatic population, some studies have shown that a lower dose of i.v rt-PA is equally efficacious. AIMS: To assess if there is a need for a dose optimization for i.v rt-PA study among Indians. SETTING AND DESIGN: A prospective, observational database of acute stroke cases that presented to a tertiary care institute over a period of 1 year was made...
January 2017: Annals of Indian Academy of Neurology
https://www.readbyqxmd.com/read/28296717/abdominal-wall-allograft-preclinical-biomechanical-investigation-of-a-novel-reconstructive-adjunct
#12
Brent R DeGeorge, Jonathan Bryce Olenczak, Angela Pineros-Fernandez, Raymond F Morgan, Patrick S Cottler, David B Drake
INTRODUCTION: Acellular dermal matrices have revolutionized abdominal wall reconstruction; however, device failure and hernia recurrence remain significant problems. Fascia grafts are a reconstructive adjunct with increased tensile strength compared with acellular dermal matrices; however, clinical use is limited by insufficient donor material and donor site morbidity. To this end, we investigate the biomechanical properties of human abdominal wall allografts (AWAs) consisting of the anterior rectus sheath from xiphoid to pubis...
March 14, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28281203/recent-advances-in-non-invasive-esophageal-tissue-sampling
#13
REVIEW
David A Katzka
PURPOSE OF REVIEW: The costs to society and accuracy of screening for esophageal cancer and other esophageal diseases with standard endoscopy are formidable. As a result, the applicability of endoscopy as a general screening tool has been challenged. RECENT FINDINGS: To maintain accuracy but reduce the price of endoscopy on society, multiple adjunct or replacement technologies are being developed that are less expensive and more easily applied. These devices include image-enhancing techniques that more reliably identify dysplasia and cancer reducing the need for extensive biopsy sampling during standard endoscopy...
March 2017: Current Gastroenterology Reports
https://www.readbyqxmd.com/read/28261617/repair-of-osteochondral-defects-using-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-in-a-rabbit-model
#14
Shuyun Liu, Yanhui Jia, Mei Yuan, Weimin Guo, Jingxiang Huang, Bin Zhao, Jiang Peng, Wenjing Xu, Shibi Lu, Quanyi Guo
Umbilical cord Wharton's jelly-derived mesenchymal stem cell (WJMSC) is a new-found mesenchymal stem cell in recent years with multiple lineage potential. Due to its abundant resources, no damage procurement, and lower immunogenicity than other adult MSCs, WJMSC promises to be a good xenogenous cell candidate for tissue engineering. This in vivo pilot study explored the use of human umbilical cord Wharton's jelly mesenchymal stem cells (hWJMSCs) containing a tissue engineering construct xenotransplant in rabbits to repair full-thickness cartilage defects in the femoral patellar groove...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28242778/%C3%AE-b-crystallin-reverses-high-diastolic-stiffness-of-failing-human-cardiomyocytes
#15
Constantijn Franssen, Jeroen Kole, René Musters, Nazha Hamdani, Walter J Paulus
BACKGROUND: Cardiomyocytes with a less distensible titin and interstitial collagen contribute to the high diastolic stiffness of failing myocardium. Their relative contributions and mechanisms underlying loss of titin distensibility were assessed in failing human hearts. METHODS AND RESULTS: Left ventricular tissue was procured in patients with aortic stenosis (AS, n=9) and dilated cardiomyopathy (DCM, n=6). Explanted donor hearts (n=8) served as controls. Stretches were performed in myocardial strips, and an extraction protocol differentiated between passive tension (Fpassive) attributable to cardiomyocytes or to collagen...
March 2017: Circulation. Heart Failure
https://www.readbyqxmd.com/read/28229416/human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-as-an-individual-specific-and-renewable-source-of-adult-stem-cells
#16
Glen Lester Sequiera, Sekaran Saravanan, Sanjiv Dhingra
This chapter deals with the employment of human-induced pluripotent stem cells (hiPSCs) as a candidate to differentiate into mesenchymal stem cells (MSCs). This would enable to help establish a regular source of human MSCs with the aim of avoiding the problems associated with procuring the MSCs either from different healthy individuals or patients, limited extraction potentials, batch-to-batch variations or from diverse sources such as bone marrow or adipose tissue. The procedures described herein allow for a guided and ensured approach for the regular maintenance of hiPSCs and their subsequent differentiation into MSCs using the prescribed medium...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28198009/endoscopic-ultrasound-guided-fine-needle-aspirate-derived-preclinical-pancreatic-cancer-models-reveal-panitumumab-sensitivity-in-kras-wild-type-tumors
#17
William Berry, Elizabeth Algar, Beena Kumar, Christopher Desmond, Michael Swan, Brendan J Jenkins, Daniel Croagh
Pancreatic cancer (PC) is largely refractory to existing therapies used in unselected patient trials, thus emphasizing the pressing need for new approaches for patient selection in personalized medicine. KRAS mutations occur in 90% of PC patients and confer resistance to epidermal growth factor receptor (EGFR) inhibitors (e.g., panitumumab), suggesting that KRAS wild-type PC patients may benefit from targeted panitumumab therapy. Here, we use tumor tissue procured by endoscopic ultrasound-guided fine-needle aspirate (EUS-FNA) to compare the in vivo sensitivity in patient-derived xenografts (PDXs) of KRAS wild-type and mutant PC tumors to panitumumab, and to profile the molecular signature of these tumors in patients with metastatic or localized disease...
February 15, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28188872/pre-transplantation-31-p-magnetic-resonance-spectroscopy-for-quality-assessment-of-human-pancreatic-grafts-a-feasibility-study
#18
Lina Carlbom, Jan Weis, Lars Johansson, Olle Korsgren, Håkan Ahlström
OBJECTIVE: To investigate the feasibility of using (31)P-MRS for objective non-invasive quality assessment of human pancreas grafts prior to transplantation or islet isolation. MATERIALS AND METHODS: Pancreata from 5 human donors, 3 males and 2 females, aged 49-78years, with body mass index (BMI) 22-31kg/m(2), were included. Pancreata were perfused with histidine-tryptophan-ketoglutarate solution during procurement and stored in hypothermic condition (4°C) for 21-44h...
February 8, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28183047/gene-expression-changes-in-colon-tissues-from-colorectal-cancer-patients-following-the-intake-of-an-ellagitannin-containing-pomegranate-extract-a-randomized-clinical-trial
#19
María A Nuñez-Sánchez, Antonio González-Sarrías, Rocío García-Villalba, Tamara Monedero-Saiz, Noelia V García-Talavera, María B Gómez-Sánchez, Carmen Sánchez-Álvarez, Ana M García-Albert, Francisco J Rodríguez-Gil, Miguel Ruiz-Marín, Francisco A Pastor-Quirante, Francisco Martínez-Díaz, Francisco A Tomás-Barberán, Juan Carlos Espín, María-Teresa García-Conesa
The clinical evidence of dietary polyphenols as colorectal cancer (CRC) chemopreventive compounds is very weak. Verification in humans of tissue-specific molecular regulation by the intake of polyphenols requires complex clinical trials that allow for the procurement of sufficient pre- and postsupplementation tissue samples. Ellagitannins (ETs), ellagic acid (EA) and their gut microbiota-derived metabolites, the urolithins, modify gene expression in colon normal and cancer cultured cells. We conducted here the first clinical trial with 35 CRC patients daily supplemented with 900 mg of an ET-containing pomegranate extract (PE) and evaluated the expression of various CRC-related genes in normal and cancerous colon tissues before (biopsies) and after (surgical specimens) 5-35 days of supplementation...
January 27, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28168823/opo-strategies-to-prevent-unintended-use-of-kidneys-exported-for-high-pra-98-cpra-recipients
#20
A S Paramesh, N Neidlinger, M Salvatore, A Smith, A Friedman, W Payne, T Taber, C Wright
Since the advent of the Kidney Allocation System (KAS), matched candidates with high (>98%) panel reactive antibody (hPRA) are given priority over local candidates with lower PRA. This often leads to exporting of kidneys. Data for these kidneys are not detailed on routine reports. Twenty-two organ procurement organizations prospectively submitted data from August 2015 to July 2016, describing allocation practices of kidneys to hPRA patients and outcomes of these kidneys. Five hundred twenty out of 6924 procured kidneys were exported for hPRA recipients...
February 6, 2017: American Journal of Transplantation
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