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Tissue Procurement

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https://www.readbyqxmd.com/read/28643729/differential-diagnoses-of-elevated-lesions-of-the-upper-lip-an-overview
#1
REVIEW
Gowri Pandarinath Bhandarkar, Kushal Vasanth Shetty
This paper comes with a purpose to help the clinician as how to arrive at a logical differential diagnosis when an upper lip mass is encountered in day-to-day practice. The labial mucosa is commonly traumatized. One must be aware of the type of structures contained in the mucosa of the area of a lesion as well as the patients habits since they may relate to a lesion presented for diagnosis. It also helps them to procure a sound knowledge of the lesions by describing the lesions according to their clinical appearance and to aid the clinician in arriving at a differential diagnosis by considering that certain lesions have a predilection for the upper lip...
April 2017: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28643577/an-assessment-of-advance-relatives-approach-for-brain-death-organ-donation
#2
Carine Michaut, Antoine Baumann, Hélène Gregoire, Corinne Laviale, Gérard Audibert, Xavier Ducrocq
BACKGROUND: Advance announcement of forthcoming brain death has developed to enable intensivists and organ procurement organisation coordinators to more appropriately, and separately from each other, explain to relatives brain death and the subsequent post-mortem organ donation opportunity. Research aim: The aim was to assess how potentially involved healthcare professionals perceived ethical issues surrounding the strategy of advance approach. RESEARCH DESIGN: A multi-centre opinion survey using an anonymous self-administered questionnaire was conducted in the six-member hospitals of the publicly funded East of France regional organ and tissue procurement network called 'Prélor'...
January 1, 2017: Nursing Ethics
https://www.readbyqxmd.com/read/28638457/upregulation-of-coxsackie-adenovirus-receptor-sensitizes-cisplatin-resistant-lung-cancer-cells-to-crad-induced-inhibition
#3
Ali Sakhawat, Yanan Liu, Ling Ma, Tahir Muhammad, Shensen Wang, Lina Zhang, Xianling Cong, Yinghui Huang
Objective. Conditionally replicating adenoviruses (CRAds) have been proven potent oncolytic viruses in previous studies. They selectively replicate in the tumor cells because of incorporated survivin promoter and ultimately lead to their killing with minimal side effects on normal tissue. Chemotherapy with cisplatin is commonly employed for treating tumors, but its cytotoxic effects and development of resistance remained major concerns to be dealt with. The aim of this study was to explore the anticancer potential of survivin regulated CRAd alone or in combination with cisplatin in the A549 lung cancer cell line and cisplatin-resistant lung cancer cell line, A549-DDPR...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28615040/immunohistochemical-and-immunocytochemical-study-of-mechanoreceptors-in-anterior-cruciate-ligament-reconstruction-with-the-remnant-preserving-technique-using-achilles-tendon-allografts
#4
Keun Churl Chun, Sung Hyun Lee, Jeong Woo Kim, Eun Jung Jin, Kwang Mee Kim, Churl Hong Chun
BACKGROUND: Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnant tissue of ACL reconstruction performed with the remnant-preserving technique. METHODS: Tissue samples were obtained from 10 patients who underwent ACL reconstruction with the remnant-preserving technique...
June 14, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28609003/preanalytic-specimen-triage-smears-cell-blocks-cytospin-preparations-transport-media-and-cytobanking
#5
Gilda da Cunha Santos, Mauro A Saieg
With increasing requests for the evaluation of prognostic and predictive molecular biomarkers, great attention must be paid to the preanalytical issues regarding sample quality and DNA/RNA yield from all different types of cytological preparations. The objectives of this review were: 1) to provide an update regarding the importance of specimen triage as well as specimen handling and collection; 2) to discuss the different cell preparations that can be used for molecular testing, their advantages and limitations; and 3) to highlight the strategies for biobanking cytology samples...
June 2017: Cancer
https://www.readbyqxmd.com/read/28602017/design-and-evaluation-of-artificial-cornea-with-core-skirt-design-using-polyhydroxyethyl-methacrylate-and-graphite
#6
Mukty Sinha, Tanvi Gupte
PURPOSE: Artificial cornea is the effective treatment option for corneal blindness. One of the challenges with the artificial cornea is limited, or no tissue integration necessitates reimplantation due to necrosis or corneal melting. We propose here a new formulation approach for core-skirt incorporating graphite in the outer skirt region to improve cell adhesion. METHODS: Hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate were procured from Sigma-Aldrich...
June 10, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28601794/obstacles-opportunities-and-priorities-for-advancing-metastatic-breast-cancer-research
#7
Margaret Flowers, Stephanie Birkey Reffey, Shirley A Mertz, Marc Hurlbert
In January 2016, the Metastatic Breast Cancer Alliance (the Alliance) convened a think tank of stakeholders from academia, government, industry, and patient advocacy to discuss gaps and opportunities in clinical and translational research in metastatic breast cancer. Priorities that emerged from the meeting included the following: the need for innovative preclinical model systems to study metastatic disease; increased sharing of resources and data; collaboration across cancer care teams and scientists; biorepositories for studies to identify biomarkers for treatment response; creation of patient registries to increase access to clinical trials and tissue procurement; and redesign of clinical trials in metastatic breast cancer...
June 10, 2017: Cancer Research
https://www.readbyqxmd.com/read/28600230/cytokine-and-chemokine-profile-changes-in-patients-with-lower-segment-lumbar-degenerative-spondylolisthesis
#8
Juraj Sutovsky, Martin Benco, Martina Sutovska, Michaela Kocmalova, Lenka Pappova, Juraj Miklusica, Andrej Frano, Egon Kurca
BACKGROUND: Lumbar degenerative spondylolisthesis (DS) develops as a result of inflammatory and remodeling processes in facet joints (FJs). Several inflammatory cytokines are involved in the osteoarthritic and remodeling changes that occur and in low-back and/or radicular pain, the most prevalent clinical symptom of disease. This study improves knowledge related to the roles that 27 cytokines, chemokines and growth factors play in the pathophysiology of lumbar DS. MATERIAL AND METHODS: Cytokine levels were examined using capture sandwich immunoassay using the Bio-Plex(®) 200 System and the Bio-PlexTM Human Cytokine Standard 27-Plex, Group I (Bio-Rad, Hercules, California, USA) separately in intervertebral discs (IVDs) and FJ bone tissue...
June 6, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28595579/omental-adipose-tissue-is-a-more-suitable-source-of-canine-mesenchymal-stem-cells
#9
Francisca Bahamondes, Estefania Flores, Gino Cattaneo, Flavia Bruna, Paulette Conget
BACKGROUND: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue...
June 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28590291/bronchoscopic-modalities-to-diagnose-sarcoidosis
#10
Sadia Benzaquen, Alejandro Adolfo Aragaki-Nakahodo
PURPOSE OF REVIEW: Several studies have investigated different bronchoscopic techniques to obtain tissue diagnosis in patients with suspected sarcoidosis when the diagnosis cannot be based on clinicoradiographic findings alone. In this review, we will describe the most recent and relevant evidence from different bronchoscopic modalities to diagnose sarcoidosis. RECENT FINDINGS: Despite multiple available bronchoscopic modalities to procure tissue samples to diagnose sarcoidosis, the vast majority of evidence favors endobronchial ultrasound transbronchial needle aspiration to diagnose Scadding stages 1 and 2 sarcoidosis...
June 5, 2017: Current Opinion in Pulmonary Medicine
https://www.readbyqxmd.com/read/28583200/high%C3%A2-quality-clinical%C3%A2-grade-human-embryonic-stem-cell-lines-derived-from-fresh-discarded-embryos
#11
Jinpei Ye, Nicola Bates, Despina Soteriou, Lisa Grady, Clare Edmond, Alex Ross, Alan Kerby, Philip A Lewis, Tope Adeniyi, Ronnie Wright, Kay V Poulton, Marcus Lowe, Susan J Kimber, Daniel R Brison
BACKGROUND: Human embryonic stem cells (hESCs) hold tremendous promise for cell replacement therapies for a range of degenerative diseases. In order to provide cost-effective treatments affordable by public health systems, HLA-matched allogeneic tissue banks of the highest quality clinical-grade hESCs will be required. However only a small number of existing hESC lines are suitable for clinical use; they are limited by moral and ethical concerns and none of them apply Good Manufacturing Practice (GMP) standards to the earliest and critical stages of gamete and embryo procurement...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28574951/single-artery-human-ear-graft-procurement-a-simplified-approach
#12
Jérôme Duisit, Hadrien Amiel, Donovan Debluts, Louis Maistriaux, Alexander Gerdom, Anne Bol, Pierre Gianello, Catherine Behets, Benoit Lengelé
BACKGROUND: In the field of experimental facial vascularized composite tissue allotransplantation (VCA), a human auricular subunit model, pedicled on both superficial temporal (STA) and posterior auricular (PAA) arteries was described. Clinical cases of extensive auricular replantation however, suggested that a single artery could perfuse the entire flap. In our study, variants of this single pedicle approach have been studied, aiming to develop a more versatile replantation technique, in which the question of venous drainage has also been addressed...
May 31, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28574036/-stimulation-of-angiogenesis-by-bone-marrow-cells-in-experimental-ischaemia-of-lower-limb
#13
B S Sukovatykh, A Iu Orlova
The objective of the study was to experimentally substantiate a possibility of treating lower limb chronic ischaemia by means of administration of bone marrow cells. The study was performed on 100 laboratory 'Wistar' rats subdivided into four groups: intact, control, first and second study groups. The intact group consisted of 10 animals, with the remaining three containing 30 rats each. All animals, except the intact ones, were subjected to modelled critical ischaemia of a hind limb by means of excision of major vessels of the extremity...
2017: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/28551055/fresh-osteochondral-allografts-procurement-and-tissue-donation-in-europe
#14
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
Fresh osteochondral allografts are a well-established treatment for large, full-thickness cartilage defects. The clinical outcome for carefully selected patients is very favorable, especially for the young and active and graft survival up to 25 years has been described in the literature. Furthermore, a high patient satisfaction rate has been reported, but the biggest obstacle to overcome is the availability of tissue for transplantation. Large fresh bone allografts for cartilage damage repair only can be harvested from organ donors following organ removal or cadaveric donors, preferably in the setting of an operation room to minimize possible contamination of the tissue...
May 11, 2017: Injury
https://www.readbyqxmd.com/read/28536798/pollutants-induced-oxidative-stress-dna-damage-and-cellular-deformities-in-clarias-gariepinus-burchell-from-river-yamuna-in-delhi-region-india
#15
Anil K Tyor, Kanika Pahwa
The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR's and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0...
May 23, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28510634/autologous-adipose-derived-tissue-matrix-part-i-biologic-characteristics
#16
Stephen A Schendel
Background: Autologous collagen is an ideal soft tissue filler and may serve as a matrix for stem cell implantation and growth. Procurement of autologous collagen has been limited, though, secondary to a sufficient source. Liposuction is a widely performed and could be a source of autologous collagen. Objectives: The amount of collagen and its composition in liposuctioned fat remains unknown. The purpose of this research was to characterize an adipose-derived tissue-based product created using ultrasonic cavitation and cryo-grinding...
May 16, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28509699/penis-transplantation-first-us-experience
#17
Curtis L Cetrulo, Kai Li, Harry M Salinas, Matthew D Treiser, Ilse Schol, Glen W Barrisford, Francis J McGovern, Adam S Feldman, Michael T Grant, Cigdem Tanrikut, Jeffrey H Lee, Richard J Ehrlichman, Paul W Holzer, Garry M Choy, Raymond W Liu, Zhi Yang Ng, Alexandre G Lellouch, Josef M Kurtz, William G Austen, Jonathan M Winograd, Branko Bojovic, Kyle R Eberlin, Ivy A Rosales, Robert B Colvin, Dicken S C Ko
OBJECTIVE: We describe the first successful penis transplant in the United States in a patient with a history of subtotal penectomy for penile cancer. BACKGROUND: Penis transplantation represents a new paradigm in restoring anatomic appearance, urine conduit, and sexual function after genitourinary tissue loss. To date, only 2 penis transplants have been performed worldwide. METHODS: After institutional review board approval, extensive medical, surgical, and radiological evaluations of the patient were performed...
May 15, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28501997/using-laser-capture-microdissection-to-isolate-cortical-laminae-in-nonhuman-primate-brain
#18
Brian A Corgiat, Claudius Mueller
Laser capture microdissection (LCM) is a technique that allows procurement of an enriched cell population from a heterogeneous tissue sample under direct microscopic visualization. Fundamentally, laser capture microdissection consists of three main steps: (1) visualizing the desired cell population by microscopy, (2) melting a thermolabile polymer onto the desired cell populations using infrared laser energy to form a polymer-cell composite (capture method) or photovolatizing a region of tissue using ultraviolet laser energy (cutting method), and (3) removing the desired cell population from the heterogeneous tissue...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28499885/the-role-of-gilz-in-modulation-of-adaptive-immunity-in-a-murine-model-of-myocardial-infarction
#19
Babak Baban, Lin Yin, Xu Qin, Jun Yao Liu, Xingming Shi, Mahmood S Mozaffari
Myocardial infarction (MI) is associated with intense immune and inflammatory responses which contribute to tissue injury. Increasing evidence indicates that the glucocorticoid-induced leucine zipper (GILZ) protein suppresses immune and inflammatory responses. However, the status of and the role of GILZ in MI are not known. We tested the hypotheses that a) MI reduces cardiac GILZ associated with intense inflammation and cell death and b) intramyocardial GILZ delivery confers cardioprotection in association with increased Tregs and suppression of inflammation...
May 10, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28497127/evidence-of-glycolysis-up-regulation-and-pyruvate-mitochondrial-oxidation-mismatch-during-mechanical-unloading-of-the-failing-human-heart-implications-for-cardiac-reloading-and-conditioning
#20
Nikolaos A Diakos, Sutip Navankasattusas, E Dale Abel, Jared Rutter, Lauren McCreath, Peter Ferrin, Stephen H McKellar, Dylan V Miller, Song Y Park, Russell S Richardson, Ralph Deberardinis, James E Cox, Abdallah G Kfoury, Craig H Selzman, Josef Stehlik, James C Fang, Dean Y Li, Stavros G Drakos
This study sought to investigate the effects of mechanical unloading on myocardial energetics and the metabolic perturbation of heart failure (HF) in an effort to identify potential new therapeutic targets that could enhance the unloading-induced cardiac recovery. The authors prospectively examined paired human myocardial tissue procured from 31 advanced HF patients at left ventricular assist device (LVAD) implant and at heart transplant plus tissue from 11 normal donors. They identified increased post-LVAD glycolytic metabolites without a coordinate increase in early, tricarboxylic acid (TCA) cycle intermediates...
October 2016: JACC. Basic to Translational Science
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