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Tissue Procurement

Hidehiko Yoshimatsu, Takuya Iida, Takumi Yamamoto, Akitatsu Hayashi
BACKGROUND:  The superficial circumflex iliac artery (SCIA)-based iliac bone flap has yet to be widely used. The purpose of this article is to validate the feasibility of SCIA-based iliac bone flap transfers for reconstruction of small to moderate-sized bony defects. Retrospective outcome comparisons between SCIA-based iliac bone flaps and fibula flaps were made. METHODS:  Twenty-six patients with bony tissue defects underwent reconstructions using either free SCIA-based iliac bone flaps (13) or fibula flaps (13)...
May 12, 2018: Journal of Reconstructive Microsurgery
Laura A Siminoff, Maureen Wilson-Genderson, Heather M Gardiner, Maghboeba Mosavel, Kathryn Laura Barker
Tissues from postmortem transplantation donors are a viable and productive option for genomic research. This entails obtaining authorization from the family decision makers (FDMs) of deceased donors. This study examined best practices for making such requests within the context of the Genotype-Tissue Expression (GTEx) project, a large national effort to collect reference tissues to establish a genomic biobank and database. Our study interviewed 413 FDMs about their donation experiences. We assessed FDM understanding of important consent concepts varied such as ability to withdraw tissues, the risks of donation, and return of results...
May 10, 2018: Biopreservation and Biobanking
Javad Hashemi, Parvin Pasalar, Masoud Soleimani, Ehsan Arefian, Reza Khorramirouz, Aram Akbarzadeh, Fariba Ghorbani, Seyed Ehsan Enderami, Abdol Mohammad Kajbafzadeh
INTRODUCTION: Diabetes is known as a worldwide disease with a great burden on society. Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered. Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells. The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ...
May 9, 2018: Cells, Tissues, Organs
Caterina Lonati, Giulia A Bassani, Daniela Brambilla, Patrizia Leonardi, Andrea Carlin, Alice Faversani, Stefano Gatti, Franco Valenza
Despite increasing clinical adoption, biologic influences of ex vivo lung perfusion (EVLP) remain insufficiently elucidated. The aim of the current study was to investigate biomolecular changes induced by EVLP in rat lungs. EVLP was maintained for 180 min. Hyaluronan, mediators, and cells were assessed in the perfusate. Gene expression, signaling pathways, and ATP content were investigated in lung tissue. EVLP induced the release of medium-high molecular weight hyaluronan and transcription of hyaluronan synthases ( P < 0...
May 2, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Tammy Z Movsas, Robert Sigler, Arivalagan Muthusamy
Purpose/Aim: Luteinizing hormone (LH) is known to function as a key regulator of VEGF expression in reproductive organs. In recent years, LH has also been detected in human vitreous and LH receptors have been identified in human retina. This study was aimed to investigate a potential correlation between LH and VEGF levels in healthy mammalian eyes to provide supporting evidence of LH's potential involvement in intraocular VEGF regulation. METHODS: 18 bovine and 30 porcine eyes were procured from an abattoire and VEGF and LH levels were measured in the vitreous extracted from these eyes by commercially-available bovine & porcine ELISA assay kits...
April 20, 2018: Current Eye Research
Ngan N Lam, Krista L Lentine, Scott Klarenbach, Manish M Sood, Paul J Kuwornu, Kyla L Naylor, Gregory A Knoll, S Joseph Kim, Ann Young, Amit X Garg
Background: Use of administrative data for outcomes assessment in living kidney donors is increasing given the rarity of complications and challenges with loss to follow-up. Objective: To assess the validity of living donor nephrectomy in health care administrative databases compared with the reference standard of manual chart review. Design: Retrospective cohort study. Setting: 5 major transplant centers in Ontario, Canada...
2018: Canadian Journal of Kidney Health and Disease
Y E Teh, M L T Ang, M V La, V Gunalan, C K Tan, A L Tan, R T P Lin, T T Tan, P R Jeyaraj, S Cumaraswamy, B H Tan
BACKGROUND: The transmission of fungi via transplant, although well-known, has not often been molecularly proven. We describe a case of donor-derived candidiasis verified by whole genome sequencing. CASE DESCRIPTION: The multiorgan donor was a 42-year-old woman with subdural hemorrhage. Procurement of the thoracic organs was performed followed by the abdominal organs. Tissue from the left bronchus grew Candida dubliniensis. The liver recipient was a 63-year-old woman with cryptogenic liver cirrhosis...
April 2018: Transplantation Proceedings
Chase A Arbra, Satish N Nadig, Sarah Grace Dennis, Sanket Pattanaik, Heather A Bainbridge, J Matthew Rhett, Stephen A Fann, Carl Atkinson, Michael J Yost
Kidney transplantation is now a mainstream therapy for end-stage renal disease. However, with approximately 96,000 people on the waiting list and only one-fourth of these patients achieving transplantation, there is a dire need for alternatives for those with failing organs. In order to decrease the harmful consequences of dialysis along with the overall healthcare costs it incurs, active investigation is ongoing in search of alternative solutions to organ transplantation. Implantable tissue-engineered renal cellular constructs are one such feasible approach to replacing lost renal functionality...
March 27, 2018: Journal of Visualized Experiments: JoVE
Chloé Michel, Rita Küchler, Iris Martin, Bruno Kyewski, Sheena Pinto
The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence reporter-based animal models with each method presenting its idiosyncratic shortcomings. Quantitative assessment of epithelial cell turnover has been partly hampered due to their variable and limited in vivo accessibility and the requirement for harsher isolation procedures to procure single cells for FACS analysis...
March 29, 2018: Journal of Immunological Methods
J De Deken, S Rex, E Lerut, W Martinet, D Monbaliu, J Pirenne, I Jochmans
BACKGROUND: Ischaemia-reperfusion injury is inevitable during renal transplantation and can lead to delayed graft function and primary non-function. Preconditioning, reconditioning and postconditioning with argon and xenon protects against renal ischaemia-reperfusion injury in rodent models. The hypothesis that postconditioning with argon or xenon inhalation would improve graft function in a porcine renal autotransplant model was tested. METHODS: Pigs (n = 6 per group) underwent left nephrectomy after 60 min of warm ischaemia (renal artery and vein clamping)...
March 30, 2018: British Journal of Surgery
Emre Gok, Fares Alghanem, Ruth Moon, Erin Guy, Alvaro Rojas-Pena, Robert H Bartlett, Kagan Ozer
Ex-situ perfusion (ESP) is a promising method in preserving vascularized composite tissue allografts (VCAs) with potential to widen donor procurement to larger geographic areas. To optimize the method of preservation, we developed a small animal model to conduct biomolecular investigations. Twenty rat hind limbs (18.2 ± 1.3 g) were procured and connected to our custom-made ESP system. Perfusion pressure and flow parameters were measured with hourly blood gas analysis under near-normothermic (30-35˚C) conditions...
March 26, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
L S M Freitas, P C Rocon, A V de Almeida, R G N Erlacher, F M Paro
BACKGROUND: In most countries, the amount of corneal graft tissue available for transplantation is insufficient to meet demand. In 2010, there were 459 patients on the waiting list for corneal transplantation (WLCT) in Espirito Santo (ES), a Brazilian state with 3,885,000 inhabitants. Several strategies were implemented to reduce the number of individuals on the WLCT, which reached zero in 2011. Studies on the evolution of corneal donation contribute to reducing the WLCT worldwide. The aim of this study was to describe the evolution of corneal donation in ES and the profile of corneal donors from 2010 to 2013...
March 2018: Transplantation Proceedings
N Marogna, R Bernardi, M Monti, G Costa, M Aguirre Mandau, J L de Echave
INTRODUCTION: The Hospital El Cruce is a high complexity center that performs transplants, both the procurement and the implant of organs and tissues. To deal with the low availability of organs and tissues from cadaveric donors it has been implemented the training through clinical simulation. OBJECTIVE: To assess if continuous training through procurement clinical simulation workshops modifies the production and quality indicators of organ and tissue procurement for transplantation in the hospital...
March 2018: Transplantation Proceedings
N Tommasino, M Saravia, A Rodriguez, R Mizraji
INTRODUCTION: The improvement in understanding the process that determines the death of an individual and his or her evolution toward brain death allows organization and planning of health policies, optimization of clinical activity and management of organ and tissue procurement processes for transplantation. OBJECTIVE: This study sought to analyze the epidemiological and evolutionary profile of patients with spontaneous subarachnoid hemorrhage (SAH) with a Glasgow Coma Scale score (GCS) ≤8 who entered follow-up in the Neurocritical Patient Monitoring Program (SPN) of Instituto Nacional de Donación y Trasplante (INDT), Uruguay...
March 2018: Transplantation Proceedings
S El Hage, M J Dos Santos, E L de Moraes, L B de Barros E Silva
INTRODUCTION: The aim of this study was to identify the percentage of bone tissue donation in a brain death situation and the tendency of donation rate of this tissue in an organ procurement organization in the county of Sao Paulo from 2001 to 2016. It is a retrospective and quantitative study, based on the Organ and Tissue Donation Term of donors who died of brain death between 2001 and 2016. METHODS: A logistic regression model was applied, and the odds of donation were identified throughout the years, regarding the odds ratio different from zero...
March 2018: Transplantation Proceedings
M J Dos Santos, E Leal de Moraes, M Santini Martins, E Carlos de Almeida, L Borges de Barros E Silva, V Urias, M C Silvano Corrêa Pacheco Furtado, Á Brito Nunes, S El Hage
BACKGROUND: The goal of this study was to identify the tendency toward donations of tissue and organs from donors with brain death between 2001 and 2016 as registered by an organ procurement organization in São Paulo City. METHODS: This quantitative, retrospective, exploratory study encompassed all Tissue and Organ Donation Terms signed between 2001 and 2016. A logistic regression model was applied to verify whether there was an upward or downward trend in donation...
March 2018: Transplantation Proceedings
C Moretti de Souza, I B de Oliveira, M J Quina Galdino, R Gvozd Costa, M Â Rossaneis, M C Fernandez Lourenço Haddad
INTRODUCTION: The high morbidity and mortality caused by chronic kidney disease, and consequently, the increase in the need for kidney transplants, makes the evaluation of the kidney donation process relevant to verifying the service's fragility with the goal of optimizing this process. OBJECTIVE: This study analyzed the process of kidney donation in the Northern Macroregional area of Paraná, Brazil. METHOD: This was a cross-sectional and retrospective evaluation study based on 586 reports of deaths provided by the Intra-Hospital Organ Donation and Transplant Tissue Commissions to the Organ Procurement Organization of the Northern Macroregional area of Paraná in Brazil between 2011 and 2015...
March 2018: Transplantation Proceedings
Asif Hasan, Colin Maciver, Gaoussou Toure, Jean-Paul Meningaud
The aesthetic outcome of a well-planned and executed vascularized composite tissue face allotransplant is unquestionable; however, complete functional restoration is necessary. The authors studied the possibility of restoring tear duct function in face transplant recipients. They also examined the technical specifics and feasibility of incorporating a lacrimal drainage apparatus into facial allografting. The authors performed a standardized face vascularized composite allograft on 4 hemi-faces (2 fresh cadavers)...
March 20, 2018: Journal of Craniofacial Surgery
Natalia D Andersen, Paula V Monje
Adult Schwann cell (SC) cultures are usually derived from nerves subjected to a lengthy step of pre-degeneration to facilitate enzymatic digestion and recovery of viable cells. To overcome the need for pre-degeneration, we developed a method that allows the isolation of adult rat sciatic nerve SCs immediately after tissue harvesting. This method combines the advantages of implementing a rapid enzymatic dissociation of the nerve fibers and a straightforward separation of cells versus myelin that improves both cell yield and viability...
2018: Methods in Molecular Biology
Yi-Guang Chen, Clayton E Mathews, John P Driver
For more than 35 years, the NOD mouse has been the primary animal model for studying autoimmune diabetes. During this time, striking similarities to the human disease have been uncovered. In both species, unusual polymorphisms in a major histocompatibility complex (MHC) class II molecule confer the most disease risk, disease is caused by perturbations by the same genes or different genes in the same biological pathways and that diabetes onset is preceded by the presence of circulating autoreactive T cells and autoantibodies that recognize many of the same islet antigens...
2018: Frontiers in Endocrinology
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