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Tissue Procurement

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https://www.readbyqxmd.com/read/29140906/nose-and-lips-graft-variants-a-subunit-anatomical-study
#1
Jérôme Duisit, Louis Maistriaux, Alexander Gerdom, Martial Vergauwen, Pierre Gianello, Catherine Behets, Benoît Lengelé
BACKGROUND: In the field of Vascularized Composite tissue Allotransplantation (VCA), the surgical design of facial subunit grafts is an evolving concept. The purpose of the present paper is to study the possibilities to divide the historical nose and lips face transplant into several morphological and functional subunit grafts, depending on their respective supply. METHODS: Our study was conducted in 20 adult cadavers. The facial artery and its branches were dissected bilaterally in 16 fresh and 4 embalmed heads...
November 11, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29131071/highly-selective-endothelin-1-receptor-a-inhibition-prevents-bleomycin-induced-pulmonary-inflammation-and-fibrosis-in-mice
#2
Nikolaos Manitsopoulos, Ioanna Nikitopoulou, Nikolaos A Maniatis, Christina Magkou, Anastasia Kotanidou, Stylianos E Orfanos
BACKGROUND: Pulmonary fibrosis is a chronic disease, which progressively leads to respiratory failure and ultimately death. Endothelin-1 (ET-1), a vasoconstrictor secreted by endothelial cells, promotes vasoconstriction by activation of its receptors A and B. OBJECTIVES: We addressed the role of highly selective ET-1 receptor A (ETA) inhibition in the pathogenesis of experimental pulmonary fibrosis by bleomycin (BLM). METHODS: BLM sulfate (2 U/mL) or saline was intratracheally administered to C57/Bl6 mice (4 groups; n = 5-11/group)...
November 9, 2017: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/29125624/non-small-cell-lung-cancer-clinical-trials-requiring-biopsies-with-biomarker-specific-results-for-enrollment-provide-unique-challenges
#3
Marshall L Spiegel, Jonathan W Goldman, Brian R Wolf, Danielle J Nameth, Tristan R Grogan, Aaron E Lisberg, Deborah J L Wong, Blanca A Ledezma, Melody A Mendenhall, Scott J Genshaft, Antonio J Gutierrez, Fereidoun Abtin, W Dean Wallace, Carlos R Adame, Jordan R McKenzie, Phillip A Abarca, Alice J Li, Jennifer L Strunck, Sina Famenini, James M Carroll, D Andrew Tucker, Lauren M Sauer, Nima M Moghadam, David A Elashoff, Christina D Abaya, Meghan B Brennan, Edward B Garon
BACKGROUND: Clinical trials in lung cancer increasingly require patients to provide fresh tumor tissue as a prerequisite to enrollment. The effects of this requirement on enrollment rates, enrollment durations, and patient selection have not been fully elucidated. METHODS: The authors retrospectively reviewed data generated by patients who consented to 1 or more interventional lung cancer clinical trials at the University of California-Los Angeles Jonsson Comprehensive Cancer Center between January 2013 and December 2014...
November 10, 2017: Cancer
https://www.readbyqxmd.com/read/29116514/combine-phage-antibody-display-library-selection-on-patient-tissue-specimens-with-laser-capture-microdissection-to-identify-novel-human-antibodies-targeting-clinically-relevant-tumor-antigens
#4
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu
A functional approach to generate tumor-targeting human monoclonal antibodies is through selection of phage antibody display libraries directly on tumor cells. Although technically convenient, the use of cancer cell lines for the selection has limitations as those cell lines often undergo genetic and epigenetic changes during prolonged in vitro culture and alter their cell surface antigen expression profile. The key is to develop a technology that allows selection of phage antibody display libraries on tumor cells in situ residing in their natural tissue microenvironment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29075760/the-nine-step-minnesota-grading-system-for-eyebank-eyes-with-age-related-macular-degeneration-a-systematic-approach-to-study-disease-stages
#5
Timothy W Olsen, Albert Liao, Hershonna S Robinson, Neal V Palejwala, Nicholas Sprehe
Purpose: To refine the Minnesota Grading System (MGS) using definitions from the Age-Related Eye Disease Studies (AREDS) into a nine-step grading scale (MGS-9). Methods: A nine-step grading scale descriptive analysis using three key phenotypic features (total drusen area, increased, and decreased pigmentation) of human eyebank eyes that were graded according to definitions from the AREDS criteria in order to harmonize studies of disease progression for research involving human tissue...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29056576/development-of-a-patient-specific-tumor-mold-using-mri-and-3d-printing-technology-for-targeted-tissue-procurement-and-radiomics-analysis-of-renal-masses
#6
Durgesh Kumar Dwivedi, Yonatan Chatzinoff, Yue Zhang, Qing Yuan, Michael Fulkerson, Rajiv Chopra, James Brugarolas, Jeffrey A Cadeddu, Payal Kapur, Ivan Pedrosa
OBJECTIVE: To implement a platform for co-localization of in vivo quantitative multi-parametric magnetic resonance imaging (mpMRI) features with ex vivo surgical specimens of patients with renal masses using patient-specific 3D-printed tumor molds, which may aid in targeted tissue procurement and radiomics/radiogenomic analyses. METHODS: Volumetric segmentation of 6 renal masses was performed with 3D Slicer (http://www.slicer.org) to create a three-dimensional (3D) tumor model...
October 19, 2017: Urology
https://www.readbyqxmd.com/read/29052751/-prevention-of-infections-due-to-musculoskeletal-allografts
#7
REVIEW
A Pruß
Transplantation of musculoskeletal tissues is widely used in the treatment of extensive defects of the musculoskeletal system, especially in orthopedics for exchange of prostheses, surgical interventions on the spine and in traumatology for reconstruction after extensive tumor resections. A danger after transplantation is the potential transmission of clinically relevant pathogens. Tissue banks have therefore established a safety level approach for musculoskeletal tissue transplants, which includes donor selection, laboratory testing, tissue procurement, tissue processing, tissue storage and quality assurance...
October 20, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28990205/developing-a-protocol-for-normothermic-ex-situ-limb-perfusion
#8
Eliana F R Duraes, Maria Madajka, Russell Frautschi, Basem Soliman, Cagri Cakmakoglu, Addison Barnett, Kashyap Tadisina, Qiang Liu, Patrick Grady, Cristiano Quintini, Toshihiro Okamoto, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
BACKGROUND: Ischemia time represents a significant limitation for successful extremity transplantation because of the rapid deterioration of ischemic muscle. Normothermic ex-situ preservation is an emergent method to prolong the organ viability following procurement, by replicating the physiologic conditions. The aim of this study was to develop an ex-situ normothermic limb perfusion system to preserve the viability and function of porcine limbs for 12 hours following procurement. METHODS: A total of 18 swine limbs were perfused...
October 9, 2017: Microsurgery
https://www.readbyqxmd.com/read/28988882/gender-proteomics-i-which-proteins-in-non-sexual-organs
#9
Elisabetta Gianazza, Ingrid Miller, Uliano Guerrini, Luca Palazzolo, Chiara Parravicini, Ivano Eberini
Differences related to gender have long been neglected but recent investigations show that they are widespread and may be recognized with all types of omics approaches, both in tissues and in biological fluids. Our review compiles evidence collected with proteomics techniques in our species, mainly focusing on baseline parameters in non-sexual organs in healthy men and women. Data from human specimens had to be replaced with information from other mammals every time invasive procedures of sample procurement were involved...
October 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28972677/hepatocellular-uptake-of-cyclodextrin-complexed-curcumin-during-liver-preservation-a-feasibility-study
#10
Saber Abdelkader Saidi, Nicolas Meurisse, Ina Jochmans, Veerle Heedfeld, Tine Wylin, Jaakko Parkkinen, Jacques Pirenne, Diethard Monbaliu, Abdelfattah El Feki, Jos van Pelt
Increasing demand for donor organs and decreasing organ quality is prompting research toward new methods to reduce ischemia reperfusion injury (IRI). Several strategies have been proposed to protect the preserved organ from this injury. Before curcumin/dextrin complex (CDC), a potent antioxidant and anti-inflammatory agent, can be used clinically we need to better understand the intracellular uptake under hypothermic conditions on rat model of liver donation after circulatory death (DCD) and brain death (DBD)...
October 3, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/28956078/-attitude-towards-organ-and-tissue-donation-in-europe-prerequisite-for-osteochondral-allograft-treatment
#11
REVIEW
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
BACKGROUND: The biggest obstacle to overcome for routine treatment of various pathologies with fresh osteochondral allograft is the availability of tissue for transplantation. Large fresh osteochondral allografts are usually harvested from organ donors, but in contrast to organs, tissues can be procured after cardiac arrest. OBJECTIVE: Medical staff as well the general public are much less aware of the possibilities and requirements of tissue donation compared to organ donation...
September 27, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28937783/validation-of-the-missouri-osteochondral-allograft-preservation-system-for-the-maintenance-of-osteochondral-allograft-quality-during-prolonged-storage
#12
Aaron M Stoker, James P Stannard, Keiichi Kuroki, Chantelle C Bozynski, Ferris M Pfeiffer, James L Cook
BACKGROUND: Fresh osteochondral allografts (OCAs) are limited in availability. The Missouri Osteochondral Allograft Preservation System (MOPS) has been reported to effectively preserve OCAs twice as long as current tissue bank protocols in preclinical studies. HYPOTHESIS: The viable chondrocyte density (VCD) in OCAs preserved for up to 70 days using the MOPS will not be significantly different from day 0, and the VCD in MOPS-preserved OCAs will be significantly higher than for standard tissue bank preservation...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28936585/understanding-intestinal-glucose-transporter-expression-in-obese-compared-to-non-obese-subjects
#13
Rebecca A Deal, Yueming Tang, Reid Fletcher, Alfonso Torquati, Philip Omotosho
INTRODUCTION: The impact of Roux-en-Y gastric bypass (RYGB) on weight loss and co-morbid disease resolution is well established. However, the mechanisms underlying the procedure remain incompletely understood. Intestinal remodeling involving glucose transporters (GLUTs) may play a crucial role. Rat studies have demonstrated morphological adaptation of GLUTs within adipose and intestinal cells in association with the reprogramming of glucose metabolism. There is a limited understanding of the variations in expression amongst GLUT family receptors in the human intestine...
September 21, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28935190/a-roadmap-for-cost-of-goods-planning-to-guide-economic-production-of-cell-therapy-products
#14
REVIEW
Yonatan Y Lipsitz, William D Milligan, Ian Fitzpatrick, Evelien Stalmeijer, Suzanne S Farid, Kah Yong Tan, David Smith, Robert Perry, Jessica Carmen, Allen Chen, Charles Mooney, John Fink
Cell therapy products are frequently developed and produced without incorporating cost considerations into process development, contributing to prohibitively costly products. Herein we contextualize individual process development decisions within a broad framework for cost-efficient therapeutic manufacturing. This roadmap guides the analysis of cost of goods (COG) arising from tissue procurement, material acquisition, facility operation, production, and storage. We present the specific COG considerations related to each of these elements as identified through a 2013 International Society for Cellular Therapy COG survey, highlighting the differences between autologous and allogeneic products...
September 18, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28924465/brain-dead-donation-rate-in-month-of-ramadan-and-the-other-months-2005-2014
#15
M Aghighi, M Mahdavi-Mazdeh, M Saberi Isfeedvajani, S A Tavakoli, N Tirgar, A Heidary Rouchi
Regardless of the level of development, religion and beliefs have crucial impact on people's attitude towards organ donation. Although organ donation in Islam is obviously appraised, mainly due to lack of an appropriate infrastructure, post-mortem donation rate in Islamic countries is not comparable to successful settings. We conducted this study to assess the extent of contribution of factors that reduce the level of effectiveness, and also to determine the impact of altruistic feelings in the month of Ramadan on family refusal as the leading modifiable contributor to organ donation rate...
2017: International Journal of Organ Transplantation Medicine
https://www.readbyqxmd.com/read/28899664/implant-for-autologous-soft-tissue-reconstruction-using-an-adipose-derived-stem-cell-colonized-alginate-scaffold
#16
Tobias Hirsch, Christine Laemmle, Bjoern Behr, Marcus Lehnhardt, Frank Jacobsen, Dirk Hoefer, Maximilian Kueckelhaus
BACKGROUND: Adipose-derived stem cells represent an interesting option for soft tissue replacement as they are easy to procure and can generate their own blood supply through the production of angiogenic factors. We seeded adipose-derived stem cells on a bioresorbable, biocompatible polymer alginate scaffold to generate autologous soft tissue constructs for repair. MATERIALS AND METHODS: We built and optimized an alginate scaffold and tested its biocompatibility using the MTT assay and its hydration capacity...
August 16, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28892876/effect-of-radiofrequency-radiation-emitted-from-2g-and-3g-cell-phone-on-developing-liver-of-chick-embryo-a-comparative-study
#17
Mary Hydrina D'Silva, Rijied Thompson Swer, J Anbalagan, Bhargavan Rajesh
INTRODUCTION: The increasing scientific evidence of various health hazards on exposure of Radiofrequency Radiation (RFR) emitted from both the cell phones and base stations have caused significant media attention and public discussion in recent years. The mechanism of interaction of RF fields with developing tissues of children and fetuses may be different from that of adults due to their smaller physical size and variation in tissue electromagnetic properties. The present study may provide an insight into the basic mechanisms by which RF fields interact with developing tissues in an embryo...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28882119/aggregation-prone-gfap-mutation-in-alexander-disease-validated-using-a-zebrafish-model
#18
So-Hyun Lee, Tai-Seung Nam, Kun-Hee Kim, Jin Hee Kim, Woong Yoon, Suk-Hee Heo, Min Jung Kim, Boo Ahn Shin, Ming-Der Perng, Hyon E Choy, Jihoon Jo, Myeong-Kyu Kim, Seok-Yong Choi
BACKGROUND: Alexander disease (AxD) is an astrogliopathy that predominantly affects the white matter of the central nervous system (CNS), and is caused by a mutation in the gene encoding the glial fibrillary acidic protein (GFAP), an intermediate filament primarily expressed in astrocytes and ependymal cells. The main pathologic feature of AxD is the presence of Rosenthal fibers (RFs), homogeneous eosinophilic inclusions found in astrocytes. Because of difficulties in procuring patient' CNS tissues and the presence of RFs in other pathologic conditions, there is a need to develop an in vivo assay that can determine whether a mutation in the GFAP results in aggregation and is thus disease-causing...
September 7, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28836741/quantification-and-neurochemical-coding-of-the-myenteric-plexus-in-humans-no-regional-variation-between-the-distal-colon-and-rectum
#19
K-S Ng, N A Montes-Adrian, D A Mahns, M A Gladman
BACKGROUND: It remains unclear whether regional variation exists in the human enteric nervous system (ENS) ie, whether intrinsic innervation varies along the gut. Recent classification of gastrointestinal neuropathies has highlighted inadequacies in the quantification of the human ENS. This study used paired wholemounts to accurately quantify and neurochemically code the hindgut myenteric plexus, comparing human distal colon and rectum. METHODS: Paired human descending colonic/rectal specimens were procured from 15 patients undergoing anterior resection...
August 24, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28836520/performance-of-a-new-needle-for-endoscopic-ultrasound-guided-fine-needle-biopsy-in-patients-with-pancreatic-solid-lesions-a-retrospective-multicenter-study
#20
Roberto Di Mitri, Mihai Rimbaş, Fabia Attili, Carlo Fabbri, Silvia Carrara, Luca Di Maurizio, Frediano Inzani, Alessandro Repici, Antonio Gasbarrini, Guido Costamagna, Alberto Larghi
BACKGROUND AND OBJECTIVES: Procurement of tissue core biopsy samples may overcome some of the limitations of endoscopic ultrasound (EUS)-guided fine-needle aspiration. We aimed at assessing the safety, histological sample procurement yield, and diagnostic accuracy of a newly available histology needle. MATERIALS AND METHODS: Data from consecutive patients with pancreatic solid lesions who underwent EUS-fine needle biopsy (EUS-FNB) using the 22-gauge Acquire™ needle were retrospectively retrieved from four tertiary care centers database...
August 24, 2017: Endoscopic Ultrasound
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