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miRNA kidney

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https://www.readbyqxmd.com/read/29159954/unraveling-the-mechanistic-complexity-of-the-glomerulocystic-phenotype-in-dicer-conditional-ko-mice-by-2d-gel-electrophoresis-coupled-mass-spectrometry
#1
Claudia Vincenza Fiumara, Domenica Scumaci, Anna Iervolino, Angela Mena Perri, Antonio Concolino, Laura Tammè, Federica Petrillo, Giovambattista Capasso, Giovanni Cuda
PURPOSE: Dicer, an RNase III type endonuclease, is a key enzyme involved in miRNA biogenesis. It has been shown that this enzyme is essential for several aspects of postnatal kidney functions and homeostasis. In this study, we have examined conditional knockout (cKO) mice for Dicer in Pax8 (Paired-box gene 8) expressing cells to investigate the kidney protein profile. This specific model develops a glomerulocystic phenotype coupled with urinary concentration impairment, proteinuria and severe renal failure...
November 21, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29154802/microrna-profiling-in-mda-mb-231-human-breast-cancer-cell-exposed-to-the-phaleria-macrocarpa-boerl-fruit-ethyl-acetate-fraction-pmeaf-through-iilumina-hi-seq-technologies-and-various-in-silico-bioinformatics-tools
#2
Nowroji Kavitha, Soundararajan Vijayarathna, Shanmugapriya, Chern Ein Oon, Yeng Chen, Jagat R Kanwar, Vasu Punj, Sreenivasan Sasidharan
ETHNOPHARMACOLOGICAL RELEVANCE: Phaleria macrocarpa (Scheff) Boerl, is a famous traditional medicinal plant which exhibited cytotoxicity against various cancerous cells. Traditionally, P. macrocarpa has been used to control cancer, impotency, hemorrhoids, diabetes mellitus, allergies, liver and heart disease, kidney disorders, blood diseases, acne, stroke, migraine, and various skin diseases. AIM OF THE STUDY: Recent studies have demonstrated a potent anticancer potential of P...
November 14, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29146158/plasma-microrna-155-5p-is-increased-among-patients-with-chronic-kidney-disease-and-nocturnal-hypertension
#3
Dominika Klimczak, Marek Kuch, Tomasz Pilecki, Dorota Żochowska, Agnieszka Wirkowska, Leszek Pączek
MicroRNAs play multiple roles in the regulation of blood pressure (BP). Nevertheless, to date, no study has assessed the association between microRNA plasma expression and BP control in chronic kidney disease (CKD) patients. Given this background, we evaluated the plasma expression of miR-155-5p, a translational inhibitor of angiotensin receptor type I, in CKD patients, to determine the association between miR-155-5p level and BP control. In this single-center cross-sectional study, we analyzed the miR-155-5p concentration by quantitative reverse transcriptase polymerase chain reaction using the U6 snRNA as a reference gene and 24-hour ambulatory blood pressure monitoring in CKD patients (stage ≥2) in relation to a control group of healthy age-matched and gender-matched individuals, with normal BP proven by the ambulatory blood pressure monitoring...
October 28, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/29137305/mir-93-3p-inhibition-suppresses-clear-cell-renal-cell-carcinoma-proliferation-metastasis-and-invasion
#4
Lu Wang, Guang Yang, Xiangwei Zhu, Ziqi Wang, Hongzhi Wang, Yang Bai, Pengcheng Sun, Li Peng, Wei Wei, Guang Chen, Guangbin Li, Andrey A Zamyatnin, Peter V Glybochko, Wanhai Xu
miRNA dysregulation is associated with many human diseases, including cancer. This study explored the effects of miR-93-3p on clear cell renal cell carcinoma (ccRCC). We found that miR-93-3p is upregulated an average of 38-fold in 138 ccRCC specimens compared to matched normal kidney tissues, which correlated with poor patient outcome. miR-93-3p inhibition reduced ccRCC cell growth, invasion, and migration in vitro and in a mouse xenograft model. A search of the TargetScan, miRanda, and PicTar databases revealed that miR-93-3p is predicted to regulate pigment epithelium-derived factor (PEDF)...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136041/profiling-analysis-of-circulating-microrna-in-peripheral-blood-of-patients-with-class-iv-lupus-nephritis
#5
Elkin Navarro-Quiroz, Lisandro Pacheco-Lugo, Roberto Navarro-Quiroz, Hernan Lorenzi, Pierine España-Puccini, Yirys Díaz-Olmos, Lisneth Almendrales, Valeria Olave, Henry Gonzalez-Torres, Anderson Diaz-Perez, Alex Dominguez, Antonio Iglesias, Raul García, Gustavo Aroca-Martinez
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It's estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals...
2017: PloS One
https://www.readbyqxmd.com/read/29127220/therapeutic-potential-of-micrornas-for-the-treatment-of-renal-fibrosis-and-ckd
#6
Wenshan Lv, Fan Fan, Yangang Wang, Ezekiel Gonzalez-Fernandez, Chen Wang, Lili Yang, George W Booz, Richard J Roman
Chronic kidney disease (CKD), defined as reduced glomerular filtration rate, is increasingly becoming a major public health issue. At the histological level, renal fibrosis is the final common pathway leading to end-stage renal disease, irrespective of the initial injury. According to this view, antifibrotic agents should slow or halt the progression of CKD. However, due to multiple overlapping pathways stimulating fibrosis, it has been difficult to develop antifibrotic drugs that delay or reverse the progression of CKD...
November 10, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/29113192/mir-514a-3p-functions-as-a-tumor-suppressor-in-renal-cell-carcinoma
#7
Lu Jin, Yifan Li, Zeng Zhang, Tao He, Jia Hu, Jiaju Liu, Mingwei Chen, Yaoting Gui, Shangqi Yang, Xiangming Mao, Yun Chen, Yongqing Lai
Renal cell carcinoma (RCC) is the most common type of kidney cancer, and the prognosis of metastatic RCC remains poor with a high rate of recurrence and mortality. A previous study has revealed that microRNA (miRNA), which negatively regulates protein expression, serves a role of oncogene or tumor suppressor. The aim of the present study was to investigate the expression and function of miR-514a-3p in RCC. To detect the expression of miR-514a-3p in 32 paired RCC tissues, quantitative polymerase chain reaction (qPCR) was performed...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29110506/mouse-population-based-evaluation-of-urinary-protein-and-mirna-biomarker-performance-associated-with-cisplatin-renal-injury
#8
Alison H Harrill, Haixia Lin, Julia Tobacyk, John C Seely
Discovery and qualification of novel biomarkers with improved specificity and sensitivity for detection of xenobiotic-induced injuries is an area of active research across multiple sectors. However, the majority of efforts in this arena have used genetically limited rodent stocks that lack variability in xenobiotic responses inherent to genetically heterogeneous human populations. In this study, genetically diverse Diversity Outbred (DO) mice were used as a surrogate for human clinical populations to investigate performance of urinary kidney biomarkers against classical preclinical kidney injury biomarkers (blood urea nitrogen [BUN] and serum creatinine)...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29109423/transcriptomic-profiling-in-human-mesangial-cells-using-patient-derived-lupus-autoantibodies-identified-mir-10a-as-a-potential-regulator-of-il8
#9
Pattarin Tangtanatakul, Boonyakiat Thammasate, Alain Jacquet, Rangsima Reantragoon, Trairak Pisitkun, Yingyos Avihingsanon, Asada Leelahavanichkul, Nattiya Hirankarn
Autoantibody-mediated inflammation directed at resident kidney cells mediates lupus nephritis (LN) pathogenesis. This study investigated the role of miRNA in human mesangial cells (HMCs) stimulated with auto anti-dsDNA immunoglobulin (Ig)G antibodies. HMCs were treated with antibodies purified from active LN patients or non-specific IgG controls in the presence of normal serum. Aberrant miRNA was screened using high throughput sequencing. Anti-dsDNA IgG up-regulated 103 miRNAs and down-regulated 30 miRNAs. The miRNAs regulated genes in the cell cycle, catabolic processes, regulation of transcription and apoptosis signalling...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29097278/hamda-hybrid-approach-for-mirna-disease-association-prediction
#10
Xing Chen, Ya-Wei Niu, Guang-Hui Wang, Gui-Ying Yan
For decades, enormous experimental researches have collectively indicated that microRNA (miRNA) could play indispensable roles in many critical biological processes and thus also the pathogenesis of human complex diseases. Whereas the resource and time cost required in traditional biology experiments are expensive, more and more attentions have been paid to the development of effective and feasible computational methods for predicting potential associations between disease and miRNA. In this study, we developed a computational model of Hybrid Approach for MiRNA-Disease Association prediction (HAMDA), which involved the hybrid graph-based recommendation algorithm, to reveal novel miRNA-disease associations by integrating experimentally verified miRNA-disease associations, disease semantic similarity, miRNA functional similarity, and Gaussian interaction profile kernel similarity into a recommendation algorithm...
October 30, 2017: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/29074972/profiling-of-mirnas-and-target-genes-related-to-cystogenesis-in-adpkd-mouse-models
#11
Yu Mi Woo, Do Yeon Kim, Nam Jin Koo, Yong-Min Kim, Sunyoung Lee, Je Yeong Ko, Yubin Shin, Bo Hye Kim, Hyowon Mun, Seonju Choi, Eun Ji Lee, Jeong-Oh Shin, Eun Young Park, Jinwoong Bok, Jong Hoon Park
Autosomal polycystic kidney disease (ADPKD) is a common inherited renal disease characterized by the development of numerous fluid-filled cysts in both kidneys. We investigated miRNA-mediated regulatory systems and networks that play an important role during cystogenesis through integrative analysis of miRNA- and RNA-seq using two ADPKD mouse models (conditional Pkd1- or Pkd2-deficient mice), at three different time points (P1, P3, and P7). At each time point, we identified 13 differentially expressed miRNAs (DEmiRs) and their potential targets in agreement with cyst progression in both mouse models...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29070573/mir-34c-5p-and-camkii-are-involved-in-aldosterone-induced-fibrosis-in-kidney-collecting-duct-cells
#12
Eui-Jung Park, Hyun Jun Jung, Hyo-Jung Choi, Jeong-In Cho, Hye-Jeong Park, Tae-Hwan Kwon
Mineralocorticoids trigger a pro-fibrotic process in kidney. In mouse cortical collecting duct cells (mpkCCDc14 cells), the present study addressed two main questions: 1) what are miRNAs and their target genes changed by aldosterone? and 2) what do miRNAs in response to aldosterone regulate signaling pathways related to fibrosis? Microarray chip assay was done in cells in the absence or presence of aldosterone treatment (10(-6)M, 3 d). The candidate miRNAs were identified by the criteria of more than 30% of fold-change among the significantly changed miRNAs (P value < 0...
October 25, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29062054/integrated-analysis-of-mrna-and-viral-mirnas-in-the-kidney-of-carassius-auratus-gibelio-response-to-cyprinid-herpesvirus-2
#13
Jianfei Lu, Dan Xu, Yousheng Jiang, Shanyun Kong, Zhaoyuan Shen, Siyao Xia, Liqun Lu
MicroRNAs (miRNAs) are small, non-coding single stranded RNAs that play crucial roles in numerous biological processes. Vertebrate herpesviruses encode multiple viral miRNAs that modulate host and viral genes. However, the roles of viral miRNAs in lower vertebrates have not been fully determined. Here, we used high-throughput sequencing to analyse the miRNA and mRNA expression profiles of Carassius auratus gibelio in response to infection by cyprinid herpesvirus 2 (CyHV-2). RNA sequencing obtained 26,664 assembled transcripts, including 2,912 differentially expressed genes...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29050002/urinary-erythrocyte-derived-mirnas-emerging-role-in-iga-nephropathy
#14
Zhi-Yu Duan, Guang-Yan Cai, Ji-Jun Li, Ru Bu, Xiang-Mei Chen
Hematuria is one of the basic clinical manifestations of IgA nephropathy (IgAN). Isolated microscopic hematuria or microscopic hematuria combined with proteinuria is risk factor for the long-term prognosis of IgAN. Current evidence of the consequences of glomerular hematuria rests on insights from basic research on the molecular mechanisms of hemoglobin and related reactive oxygen species-induced tubular injury as well as on the clinical evidence of macroscopic hematuria-associated acute kidney injury (AKI) in IgAN...
October 19, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29042600/dynamic-and-modularized-microrna-regulation-and-its-implication-in-human-cancers
#15
Jiang Shu, Bruno Vieira Resende E Silva, Tian Gao, Zheng Xu, Juan Cui
MicroRNA is responsible for the fine-tuning of fundamental cellular activities and human disease development. The altered availability of microRNAs, target mRNAs, and other types of endogenous RNAs competing for microRNA interactions reflects the dynamic and conditional property of microRNA-mediated gene regulation that remains under-investigated. Here we propose a new integrative method to study this dynamic process by considering both competing and cooperative mechanisms and identifying functional modules where different microRNAs co-regulate the same functional process...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29038788/urinary-exosomal-microrna-profiling-in-incipient-type-2-diabetic-kidney-disease
#16
Yijun Xie, Yijie Jia, Xie Cuihua, Fang Hu, Meng Xue, Yaoming Xue
BACKGROUND: Albuminuria is an early sign but not a strong predictor of diabetic kidney disease (DKD). Owing to their high stability, urinary exosomal miRNAs can be useful predictors of the progression of early-stage DKD to renal failure; fluid biopsies are ideal for detecting abnormalities in these miRNAs. The aim of this study was to identify novel differentially expressed miRNAs as urine biomarkers for type 2 DKD by comparing between patients of type 2 diabetes (T2D) with and without macroalbuminuria...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29036190/clusternomics-integrative-context-dependent-clustering-for-heterogeneous-datasets
#17
Evelina Gabasova, John Reid, Lorenz Wernisch
Integrative clustering is used to identify groups of samples by jointly analysing multiple datasets describing the same set of biological samples, such as gene expression, copy number, methylation etc. Most existing algorithms for integrative clustering assume that there is a shared consistent set of clusters across all datasets, and most of the data samples follow this structure. However in practice, the structure across heterogeneous datasets can be more varied, with clusters being joined in some datasets and separated in others...
October 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/29034845/predicting-mirna-association-with-corresponding-target-genes-and-single-nucleotide-polymorphisms-in-altered-renal-pathophysiology
#18
Pradeep Jaswani, Swayam Prakash, Narayan Prasad, Suraksha Agrawal, Raj Kumar Sharma
BACKGROUND: Translational research on miRNAs develops reliable biomarkers for diagnosis and prognosis of renal diseases. Bioinformatic analyses and systems biology could drive the research for knowing new informative miRNA targets. OBJECTIVES: This study proposes an approach to identify miRNA specific significant target genes, and single nucleotide polymorphisms (SNPs) associated with renal pathophysiology. METHODS: miRNAs were selected after removing duplicity, on the basis of techniques used, and disease spectrum width score...
October 16, 2017: MicroRNA
https://www.readbyqxmd.com/read/29031207/microrna-25-inhibits-high-glucose-induced-apoptosis-in-renal-tubular-epithelial-cells-via-pten-akt-pathway
#19
Huicong Li, Xiaoguang Zhu, Junwei Zhang, Jun Shi
Diabetic nephropathy (DN) has become the major cause of end-stage renal disease (ESRD). It has been demonstrated that apoptosis of renal tubular epithelial cells induced by hyperglycemia contributes to the pathogenesis of DN. Recent researches have corroborated the critical roles of microRNAs (miRNAs) in the apoptosis of various types of cells including renal tubular epithelial cells. However, the eff ; ;ect of miRNAs on the hyperglycemia-induced apoptosis of renal tubular epithelial cells remains unclear...
October 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29021386/suppression-of-microrna-activity-in-kidney-collecting-ducts-induces-partial-loss-of-epithelial-phenotype-and-renal-fibrosis
#20
Sachin Hajarnis, Matanel Yheskel, Darren Williams, Thomas Brefort, Bob Glaudemans, Huguette Debaix, Michel Baum, Olivier Devuyst, Vishal Patel
microRNAs (miRNAs) are sequence-specific inhibitors of post-transcriptional gene expression. The physiologic function of these noncoding RNAs in postnatal renal tubules still remains unclear. Surprisingly, they appear to be dispensable for mammalian proximal tubule (PT) function. Here, we examined the effects of miRNA suppression in collecting ducts (CDs). To conclusively evaluate the role of miRNAs, we generated three mouse models with CD-specific inactivation of key miRNA pathway genes Dicer, Dgcr8, and the entire Argonaute gene family (Ago1, 2, 3, and 4)...
October 11, 2017: Journal of the American Society of Nephrology: JASN
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