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https://www.readbyqxmd.com/read/28817834/mdm2-promotes-epithelial-mesenchymal-transition-and-metastasis-of-ovarian-cancer-skov3-cells
#1
Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang
BACKGROUND: Metastasis accounts for the most lethal reason for the death of ovarian cancer patients, but remains largely untreated. Epithelial-mesenchymal transition (EMT) is critical for the conversion of early-stage ovarian tumours into metastatic malignancies. Thus the exploration of the signalling pathways promoting EMT would open potential opportunities for the treatment of metastatic ovarian cancer. Herein, the putative role of MDM2 in regulating EMT and metastasis of ovarian cancer SKOV3 cells was investigated...
August 17, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28817830/microrna-200a-inhibits-transforming-growth-factor-%C3%AE-1-induced-proximal-tubular-epithelial-mesenchymal-transition-by-targeting-%C3%AE-catenin
#2
Yi Gong, Zhexue Qin, Baoshang Zhou, Hua Chen, Zhengmin Shi, Jing Zhang
BACKGROUND: The epithelial-mesenchymal transition (EMT) is a crucial event in the development of renal interstitial fibrosis (RIF). A growing body of evidence indicates that β-catenin plays an important role in various types of fibrosis. Although members of the microRNA (miRNA)-200 family have been suggested to suppress EMT in cancer and fibrosis, the function of miRNA-200a in regulating the progression of RIF is unknown. We speculate that miRNA-200a may hinder this progression through the suppression of β-catenin...
August 18, 2017: Nephron
https://www.readbyqxmd.com/read/28817771/ice-templated-and-cross-linked-amyloid-fibril-aerogel-scaffolds-for-cell-growth
#3
Gustav Nyström, Wye-Khay Fong, Raffaele Mezzenga
Amyloid fibrils prepared from β-lactoglobulin were used to form freeze-dried and cross-linked aerogels. By varying the fibril concentration and freezing gradient, it was possible to control the pore structure and elastic modulus of the aerogels over one order of magnitude from ~2 to ~200 kPa. Using butane tetracarboxylic acid as cross-linker these aerogels maintained their monolithic shape in aqueous conditions displaying elastic behavior and a modulus in the range of 4-40 kPa. When explored as scaffolds for cell growth, the amyloid fibril aerogels demonstrated biocompatibility and led to the successful penetration and permeation of two epithelial cell lines (Caco-2 and HT29) throughout the scaffold...
August 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28817737/myc-mediates-cancer-stem-like-cells-and-emt-changes-in-triple-negative-breast-cancers-cells
#4
Shuping Yin, Vino T Cheryan, Liping Xu, Arun K Rishi, Kaladhar B Reddy
Women with triple negative breast cancer (TNBC) have poor prognosis compared to other breast cancer subtypes. There were several reports indicating racial disparity in breast cancer outcomes between African American (AA) and European American (EA) women. For example, the mortality rates of AA breast cancer patients were three times higher than of EA patients, even though, the incidence is lower in AA women. Our in vitro studies indicate that cancer stem-like cells (CSCs) derived from AA TNBC cell lines have significantly higher self-renewal potential (mammosphere formation) than CSCs derived from EA cell lines...
2017: PloS One
https://www.readbyqxmd.com/read/28817391/tacrolimus-eye-drops-as-adjunct-therapy-in-severe-corneal-endothelial-rejection-refractory-to-corticosteroids
#5
Reza Ghaffari, Hamed Ghassemi, Mehran Zarei-Ghanavati, Golshan Latifi, Shima Dehghani, Zeeshan Haq, Ali R Djalilian
PURPOSE: To evaluate the safety and efficacy of tacrolimus eye drops as adjunctive therapy in the treatment of severe corneal endothelial rejection after penetrating keratoplasty refractory to corticosteroids. METHODS: In this prospective interventional case series, 11 eyes of 11 patients assessed for severe corneal endothelial rejection, with an inadequate response to topical, local, and systemic corticosteroids, were treated with either 0.01% or 0.05% tacrolimus eye drops 4 times daily...
August 16, 2017: Cornea
https://www.readbyqxmd.com/read/28817374/long-term-survival-outcomes-of-cancer-directed-surgery-for-malignant-pleural-mesothelioma-propensity-score-matching-analysis
#6
David B Nelson, David C Rice, Jiangong Niu, Scott Atay, Ara A Vaporciyan, Mara Antonoff, Wayne L Hofstetter, Garrett L Walsh, Stephen G Swisher, Jack A Roth, Anne Tsao, Daniel Gomez, Sharon H Giordano, Reza Mehran, Boris Sepesi
Purpose Small observational studies have shown a survival advantage to undergoing cancer-directed surgery for malignant pleural mesothelioma (MPM); however, it is unclear if these results are generalizable. Our purpose was to evaluate survival after treatment of MPM with cancer-directed surgery and to explore the effect surgery interaction with chemotherapy or radiation therapy on survival by using the National Cancer Database. Patients and Methods Patients with microscopically proven MPM were identified within the National Cancer Database (2004 to 2014)...
August 17, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28817360/closing-the-gap-mouse-models-to-study-adhesion-in-secondary-palatogenesis
#7
K J Lough, K M Byrd, D C Spitzer, S E Williams
Secondary palatogenesis occurs when the bilateral palatal shelves (PS), arising from maxillary prominences, fuse at the midline, forming the hard and soft palate. This embryonic phenomenon involves a complex array of morphogenetic events that require coordinated proliferation, apoptosis, migration, and adhesion in the PS epithelia and underlying mesenchyme. When the delicate process of craniofacial morphogenesis is disrupted, the result is orofacial clefting, including cleft lip and cleft palate (CL/P). Through human genetic and animal studies, there are now hundreds of known genetic alternations associated with orofacial clefts; so, it is not surprising that CL/P is among the most common of all birth defects...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28817352/arhgap29-mutation-is-associated-with-abnormal-oral-epithelial-adhesions
#8
B J Paul, K Palmer, J C Sharp, C H Pratt, S A Murray, M Dunnwald
Nonsyndromic cleft lip and/or palate (NSCL/P) is a prevalent birth defect of complex etiology. Previous studies identified mutations in ARHGAP29 associated with an increased risk for NSCL/P. To investigate the effects of ARHGAP29 in vivo, we generated a novel murine allele by inserting a point mutation identified in a patient with NSCL/P. This single-nucleotide variation of ARHGAP29 translates to an early nonsense mutation (K326X), presumably resulting in loss-of-function (LoF). Embryos from Arhgap29(K326X/+) intercrosses were harvested at various time points...
August 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28817343/suppression-of-choroidal-neovascularization-in-mice-by-subretinal-delivery-of-multigenic-lentiviral-vectors-encoding-anti-angiogenic-micrornas
#9
Anne Louise Askou, Josephine Natalia Esther Benckendorff, Andreas Holmgaard, Tina Storm, Lars Aagaard, Toke Bek, Jacob Giehm Mikkelsen, Thomas Juhl Corydon
Lentivirus-based vectors have been used for the development of potent gene therapies. Here, application of a multigenic lentiviral vector (LV) producing multiple anti-angiogenic microRNAs following subretinal delivery in a laser-induced choroidal neovascularization (CNV) mouse model is presented. This versatile LV, carrying back-to-back RNApolII-driven expression cassettes, enables combined expression of microRNAs targeting vascular endothelial growth factor A (Vegfa) mRNA and fluorescent reporters. In addition, by including a vitelliform macular dystrophy 2 (VMD2) promoter, expression of microRNAs is restricted to the retinal pigment epithelial (RPE) cells...
August 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/28817313/ccr7-mediates-tgf-%C3%AE-1-induced-human-malignant-glioma-invasion-migration-and-epithelial-mesenchymal-transition-by-activating-mmp2-9-through-the-nuclear-factor-kappab-signaling-pathway
#10
Yanyan Zheng, Yiting Miu, Xiaokai Yang, Xiaoguo Yang, Meijia Zhu
Chemokine receptor 7 (CCR7) has emerged as an inducer of invasion, migration, and epithelial-mesenchymal transition (EMT) in cancer. In this research, human malignant glioma cells were stimulated with transforming growth factor beta 1 (TGF-β1) and siCCR7. The data show that CCR7 mediates TGF-β1-induced EMT, migration, and invasion in U251 and U87 cells and that these effects of TGF-β1 were reversed by treatment with siCCR7 or a CCR7 neutralizing antibody. Importantly, the TGF-β1-mediated increase in nuclear factor kappaB (NF-κB) activity in human glioma cells was reduced by treatment with siCCR7 or a CCR7 neutralizing antibody...
August 17, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28817141/effects-of-platelet-rich-fibrin-on-palatal-wound-healing-after-free-gingival-graft-harvesting-a-comparative-randomized-controlled-clinical-trial
#11
Mustafa Ozcan, Onur Ucak, Bahar Alkaya, Seray Keceli, Gulsah Seydaoglu, M Cenk Haytac
This study was conducted to evaluate the effects of platelet-rich fibrin (PRF) on palatal wound healing after free gingival graft (FGG) harvesting. A total of 125 patients were randomized after FGG: PRF with butyl-cyanoacrylate (BC) adhesive (PRF group; n = 42), BC adhesive alone (BC group; n = 42), and sterile wet gauze compression (WG group; n = 41). Bleeding, pain, epithelialization, feeding habits, and sensation scores were recorded for different time points. Statistically significant differences were found for all parameters in favor of the PRF group (P = ...
September 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28817140/relationship-between-clinical-and-histologic-periodontal-biotypes-in-humans
#12
Sergio Henrique Gonçalves Motta, Marcos Paulo Ferreira Camacho, Danielle Carvalho Quintela, Ronaldo B Santana
Histologic and histomorphometric evaluations of gingival biopsy samples of patients presenting either a thick or a thin gingival biotype were performed. Full-thickness gingival biopsy samples were obtained and processed for histologic and histometric evaluations. Thickness of the keratinized mucosa or gingiva was found to be increased in the thick biotype. Specifically, the connective tissue layer was thicker with no change in epithelial thickness. In conclusion, gingival transparency appears to be an adequate, clinically useful method to classify the gingival biotype...
September 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28817128/epithelial-inclusions-following-a-bilaminar-root-coverage-procedure-with-a-subepithelial-connective-tissue-graft-a-histologic-and-clinical-study
#13
Federica Romano, Stefano Perotto, Luca Cricenti, Stefano Gotti, Mario Aimetti
The aim of this study was to histologically examine any epithelial cell inclusions in submerged subepithelial connective tissue graft (SCTG) after clinical healing was achieved. A total of 16 patients with Miller Class I or II gingival recessions were consecutively treated with a bilaminar procedure. At 2 months after surgery, a gingival tissue specimen was harvested from all SCTG-treated sites and stained with hematoxylin-eosin. The histologic evaluation revealed connective tissue in active reorganization without epithelial inclusions in 14 of the 16 tissue specimens...
September 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28817119/mir-1301-inhibits-hepatocellular-carcinoma-cell-migration-invasion-and-angiogenesis-by-decreasing-wnt-%C3%AE-catenin-signaling-through-targeting-bcl9
#14
Chao Yang, Yonghua Xu, Feng Cheng, Yuanchang Hu, Shikun Yang, Jianhua Rao, Xuehao Wang
Metastasis is the major cause of the poor prognosis of hepatocellular carcinoma (HCC), and increasing evidence supports the contribution of miRNAs to cancer progression. However, the exact relationship between the level of miR-1301 expression and HCC cell migration, invasion, and angiogenesis remains largely unknown. Quantitative PCR was used to evaluate the level of miR-1301 expression in HCC tissues and cell lines. Transwell and tube-formation assays were used to measure the effects of miR-1301 on HCC cell migration and invasion, and angiogenesis, respectively...
August 17, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28816970/skin-metastases-in-epithelial-ovarian-and-fallopian-tube-carcinoma
#15
Isao Otsuka, Takuto Matsuura
This study aimed to investigate the clinical features and outcomes of skin metastasis in ovarian and fallopian tube carcinomas.We studied patients with epithelial ovarian or fallopian tube carcinoma who developed skin metastasis from 2001 through 2012, and were also treated with chemotherapy and/or surgery.Skin metastases were classified as umbilical metastasis (Sister Joseph nodule [SJN]) and nonumbilical metastasis. Patients who developed skin metastases at paracentesis sites were excluded.Of the 206 patients treated, 12 (5...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28816710/establishment-of-primary-cell-culture-from-ascitic-fluid-and-solid-tumor-obtained-from-epithelial-ovarian-carcinoma-patients
#16
Rajarshi Kar, Diwesh Chawla, Bindiya Gupta, Mohit Mehndiratta, Neelam Wadhwa, Rachna Agarwal
OBJECTIVE: Ovarian cancer is the seventh leading cause of cancer death worldwide. This is mainly due to late diagnosis and high rate of relapse and resistance following chemotherapy. In the present study, we describe simple and cost-effective method to establish primary culture from ascitic fluid and solid tumor obtained from epithelial ovarian carcinoma patient, which may provide a better tool for in vitro testing of drug sensitivity and designing individualized treatment protocol. METHODS: Complete Dulbecco modified Eagle medium (DMEM) was prepared by supplementing DMEM with 10% fetal bovine serum and antibiotics (ciprofloxacin and amphotericin B)...
August 16, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28816640/exosomal-microrna-communication-between-tissues-during-organogenesis
#17
Toru Hayashi, Matthew P Hoffman
Epithelial-mesenchymal interactions are required to coordinate cell proliferation, patterning, and functional differentiation of multiple cell types in a developing organ. This exquisite coordination is dependent on various secreted molecules that provide developmental signals to mediate these tissue interactions. Recently, it was reported that mature mesenchymal-derived microRNAs (miRNAs) in the fetal mouse salivary gland are loaded into exosomes, and transported to the epithelium where they influence progenitor cell proliferation...
August 17, 2017: RNA Biology
https://www.readbyqxmd.com/read/28816557/cd44-as-a-cancer-stem-cell-marker-and-its-prognostic-value-in-patients-with-ovarian-carcinoma
#18
Alena Bartakova, Kveta Michalova, Jiri Presl, Pavel Vlasak, Jan Kostun, Jiri Bouda
The aim of our study was to clarify whether the CD44 adhesion molecule as a cancer stem cell marker could also serve as a prognostic factor in patients with epithelial ovarian cancer (EOC). A retrospective study was performed on 87 patients with histologically verified EOC. Specimens of both primary tumour and implantation metastases were tested from 48 of them. CD44 expression was detected by immunohistochemistry. We looked for the cut-off levels of CD44 expression using the Cox regression model. We confirmed statistically significant prognostic factors for overall survival (OS) and disease-free interval (DFI) to be: stage of the disease, postoperative residual tumour and papillary serous histological type...
August 17, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28816360/c-c-motif-chemokine-ligand-2-regulates-lps-induced-inflammation-and-er-stress-to-enhance-proliferation-of-bovine-endometrial-epithelial-cells
#19
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Sung-Man Kim, Gwonhwa Song
Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C-C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28816019/itraq-based-quantitative-proteomic-analyses-of-high-grade-esophageal-squamous-intraepithelial-neoplasia
#20
Jingjing Zhang, Chunchun Zhi, Fuxi Zhen, Xiaoqin Yuan, Chunhua Jiao, Hong Zhu, Hui Zhu, Yadong Feng
PURPOSE: Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide and is the fourth most lethal cancer in China. Little is known about the proteome of high grade esophageal squamous intraepithelial neoplasia (HGN), which is a premalignant lesion of ESCC. EXPERIMENTAL DESIGN: A quantitative proteomic analysis using an Isobaric Tags for Relative and Absolute Quantification (iTRAQ) approach was used to characterize the protein expression profiles in HGN...
August 16, 2017: Proteomics. Clinical Applications
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