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https://www.readbyqxmd.com/read/28222255/stemness-signature-of-equine-marrow-derived-mesenchymal-stem-cells
#1
Morteza Zahedi, Abbas Parham, Hesam Dehghani, Hossein Kazemi Mehrjerdi
Background: Application of competent cells such as mesenchymal stem cells (MSCs) for treatment of musculoskeletal disorders in equine athletes is increasingly needed. Moreover, similarities of horse and human in size, load and types of joint injuries, make horse as a good model for MSCs therapy studies. This study was designed to isolate and characterize stemness signature of equine bone marrow-derived mesenchymal stem cells (BM-MSCs). Methods: BM of three mares was aspirated and the mononuclear cells (MNCs) were isolated using density gradient...
February 28, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28178774/a-retrospective-review-of-rattlesnake-bites-in-100-children
#2
Norberto Sotelo-Cruz, Norberto Gómez-Rivera
BACKGROUND: A retrospective review of clinical features and treatment of children hospitalized for rattlesnake bite. METHODS: One hundred clinical records were reviewed. Variables included: age, gender, season of the year, signs, symptoms, poisoning grade, complications, treatment and sequelae. RESULTS: Fifty-nine percent were males and 37% were less than 5 years of age; 87% occurred in rural areas; 63% of the snakebites occurred during the summer, of them, 39% occurred within the perimeter of the domicile and 8% within the home...
April 2017: Minerva Pediatrica
https://www.readbyqxmd.com/read/28167587/snake-bite-induced-acute-kidney-injury-report-of-a-successful-outcome-during-pregnancy
#3
Sanjay Vikrant, Anupam Parashar
Snake bite is an important health hazard in tropics. Snake envenomation in pregnancy may cause fetal death and maternal mortality or morbidity. However, little is known about the toxic effects and optimal management during pregnancy after snake envenomation because of the rarity of cases. Herein, we report a case of a pregnant woman who was successfully treated for snake bite-induced acute kidney injury during the third trimester. She was treated with equine-derived polyvalent anti-snake venom without development of any adverse effects, hemodialysis, and supportive therapy...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28165801/use-of-polyvalent-equine-anti-viper-serum-to-treat-delayed-coagulopathy-due-to-suspected-sistrurus-miliarius-streckeri-envenomation-in-two-children
#4
Kristie L Williams, Megan Woslager, Stephanie L Garland, Roger P Barton, William Banner
BACKGROUND: Western Pygmy Rattlesnake (WPR) envenomation reportedly causes refractory and persistent coagulopathy when treated with CroFab(®) (Crotalidae Polyvalent Immune Fab). We report two cases where polyvalent equine anti-viper serum (AntivipmynTRI(®)) was used to treat recurrent coagulopathy in children. CASE DETAILS: The first patient was a 16-month-old male who was bitten by a confirmed WPR. The patient received a total of 18 vials of CroFab(®). His labs normalized, swelling gradually improved, and the child was discharged to home...
February 6, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28147452/an-open-trial-of-equine-assisted-therapy-for-children-exposed-to-problematic-parental-substance-use
#5
Menka Tsantefski, Lynne Briggs, Jessica Griffiths, Anne Tidyman
Children exposed to problematic parental substance use (PPSU) often face a number of deleterious developmental outcomes, yet these children are less likely to become known to child protection and welfare services. Although there is a growing evidence base for equine-assisted therapy (EAT) as an effective treatment modality for atypically developing children and adolescents, scant research has explored the benefit of EAT for children exposed to PPSU. The current study is the first to explore the benefit of EAT for children exposed to PPSU in Victoria, Australia...
February 1, 2017: Health & Social Care in the Community
https://www.readbyqxmd.com/read/28127949/glycolysis-inhibition-improves-photodynamic-therapy-response-rates-for-equine-sarcoids
#6
J P Golding, J G Kemp-Symonds, J M Dobson
Photodynamic therapy (PDT) holds great promise in treating veterinary and human dermatological neoplasms, including equine sarcoids, but is currently hindered by the amount of photosensitiser and light that can be delivered to lesions thicker than around 2 mm, and by the intrinsic antioxidant defences of tumour cells. We have developed a new PDT technique that combines an efficient transdermal penetration enhancer solution, for topical delivery of 5-aminolevulinic acid (ALA) photosensitiser, with acute topical post-PDT application of the glycolysis inhibitor lonidamine...
January 27, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28105630/histopathological-features-of-endometritis-eosinophilica-in-mares
#7
Anna Lena Grimm, Heinz-Adolf Schoon, Sandra Schöniger
Equine endometritis eosinophilica (EE) is rarely described and its diagnostic criteria are not defined. The aim of this study was to characterize histological features of EE. A data base (1995-2013) was searched for biopsies with increased eosinophils. This study included all biopsies with this diagnosis and representative biopsies without this record. The definition of equine EE was based on criteria for EE in women and the results of the determination of physiological numbers of eosinophils within the equine endometrium...
January 20, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28103732/evaluation-of-antivenom-therapy-for-vipera-palaestinae-bites-in-children-experience-of-two-large-tertiary-care-pediatric-hospitals
#8
Dikla Pivko-Levy, Itamar Munchnak, Ayelet Rimon, Uri Balla, Dennis Scolnik, Christopher Hoyte, Yair Voliovitch, Miguel Glatstein
BACKGROUND: Antivenom has been successfully used to treat systemic and progressive, local manifestations of envenomation inflicted by Vipera (V.) palaestinae, the most common venomous snake in Israel. The objective of this study was to evaluate the fixed dose V. palaestinae monovalent (equine) immunoglobulin G antivenom used in two pediatric emergency departments. In particular, we wanted to assess the need for repeated antivenom administration and the rate of adverse antivenom effects in children...
January 20, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28077153/detection-of-strongylus-vulgaris-in-equine-faecal-samples-by-real-time-pcr-and-larval-culture-method-comparison-and-occurrence-assessment
#9
A Kaspar, K Pfister, M K Nielsen, C Silaghi, H Fink, M C Scheuerle
BACKGROUND: Strongylus vulgaris has become a rare parasite in Germany during the past 50 years due to the practice of frequent prophylactic anthelmintic therapy. To date, the emerging development of resistance in Cyathostominae and Parascaris spp. to numerous equine anthelmintics has changed deworming management and the frequency of anthelmintic usage. In this regard, reliable detection of parasitic infections, especially of the highly pathogenic S. vulgaris is essential. In the current study, two diagnostic methods for the detection of infections with S...
January 11, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28056978/short-term-storage-stability-at-room-temperature-of-two-different-platelet-rich-plasma-preparations-from-equine-donors-and-potential-impact-on-growth-factor-concentrations
#10
Gregor Hauschild, Florian Geburek, Georg Gosheger, Maria Eveslage, Daniela Serrano, Arne Streitbürger, Sara Johannlükens, Dirk Menzel, Reinhard Mischke
BACKGROUND: The increasing interest in platelet-rich plasma (PRP) based therapies is as yet accompanied by inconsistent information regarding nearly all aspects of handling and application. Among these storage stability of processed platelet-rich products may be the basis for a more flexible application mode. The objective of this study was (1) to estimate the storage stability of growth factors platelet derived growth factor BB (PDGF-BB) and transforming growth factor ß1 (TGF-ß1) in both, a single-step softspin centrifugation-based pure-PRP (P-PRP, ACP®), and a gravity filtration system-based leukocyte-rich-PRP (L-PRP, E-PET), over a six hours time span after preparation at room temperature and (2) to identify possible factors influencing these growth factor concentrations in an equine model...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28008302/benefits-of-hormone-therapy-estrogens-depend-on-estrogen-type-17%C3%AE-estradiol-and-conjugated-equine-estrogens-have-differential-effects-on-cognitive-anxiety-like-and-depressive-like-behaviors-and-increase-tryptophan-hydroxylase-2-mrna-levels-in-dorsal-raphe-nucleus
#11
Ryoko Hiroi, Giulia Weyrich, Stephanie V Koebele, Sarah E Mennenga, Joshua S Talboom, Lauren T Hewitt, Courtney N Lavery, Perla Mendoza, Ambra Jordan, Heather A Bimonte-Nelson
Decreased serotonin (5-HT) function is associated with numerous cognitive and affective disorders. Women are more vulnerable to these disorders and have a lower rate of 5-HT synthesis than men. Serotonergic neurons in the dorsal raphe nucleus (DRN) are a major source of 5-HT in the forebrain and play a critical role in regulation of stress-related disorders. In particular, polymorphisms of tryptophan hydroxylase-2 (TpH2, the brain-specific, rate-limiting enzyme for 5-HT biosynthesis) are implicated in cognitive and affective disorders...
2016: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/27999340/equine-immunoglobulin-and-equine-neutralizing-f-ab-%C3%A2-protect-mice-from-west-nile-virus-infection
#12
Jiannan Cui, Yongkun Zhao, Hualei Wang, Boning Qiu, Zengguo Cao, Qian Li, Yanbo Zhang, Feihu Yan, Hongli Jin, Tiecheng Wang, Weiyang Sun, Na Feng, Yuwei Gao, Jing Sun, Yanqun Wang, Stanley Perlman, Jincun Zhao, Songtao Yang, Xianzhu Xia
West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and evaluated the protective efficacy of immune serum IgG and pepsin-digested F(ab')₂ fragments from horses immunized with the WNV virus-like particles (VLP) expressing the WNV M and E proteins. Immune equine F(ab')₂ fragments and immune horse sera efficiently neutralized WNV infection in tissue culture...
December 18, 2016: Viruses
https://www.readbyqxmd.com/read/27938355/metalloproteinases-and-their-inhibitors-are-influenced-by-inhalative-glucocorticoid-therapy-in-combination-with-environmental-dust-reduction-in-equine-recurrent-airway-obstruction
#13
Ann Kristin Barton, Tarek Shety, Angelika Bondzio, Ralf Einspanier, Heidrun Gehlen
BACKGROUND: Overexpression of matrix-metalloproteinases (MMPs) has been shown to lead to tissue damage in equine recurrent airway obstruction (RAO), as a misbalance with their natural inhibitors, the tissue inhibitors of metalloproteinases (TIMPs), occurs. This favors irreversible pulmonary fibrosis formation. Increased levels of MMPs, TIMPs or altered ratios between them can be used as biomarkers of respiratory disease. We hypothesized that levels of MMPs, TIMPs and their ratios correlate with improvement in clinical findings and bronchoalveolar lavage fluid (BALF) cytology after 10 days of inhalative glucocorticoid therapy and environmental dust reduction (EDR) and may be used to monitor treatment success...
December 9, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27931511/myod-overexpressed-equine-adipose-derived-stem-cells-enhanced-myogenic-differentiation-potential
#14
Soo-Eun Sung, Meeyul Hwang, Ah-Young Kim, Eun-Mi Lee, Eun-Joo Lee, Su-Kyeong Hwang, Shin-Yoon Kim, Hong-Kyun Kim, Kyu-Shik Jeong
Mesenchymal stem cells could potentially be used in the clinical treatment of muscle disorders and muscle regeneration. Adipose-derived stem cells (ADSCs) can be easily isolated from adipose tissue, as opposed to stem cells of other tissues. We believe that cell therapy using ADSCs could be applied to muscle disorders in horses and other species. We sought to improve the myogenic differentiation potential of equine ADSCs (eqADSCs) using a MyoD lentiviral vector. MyoD lentiviruses were transduced into eqADSCs and selected using puromycin...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27901391/comparison-of-antibacterial-effects-among-three-foams-used-with-negative-pressure-wound-therapy-in-an-ex-vivo-equine-perfused-wound-model
#15
COMPARATIVE STUDY
Lore L Van Hecke, Maarten Haspeslagh, Katleen Hermans, Ann M Martens
OBJECTIVE To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. SAMPLES Abdominal musculocutaneous flaps from 6 equine cadavers. PROCEDURES Each musculocutaneous flap was continuously perfused with saline (0.9% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 10(6) CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound...
December 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27900987/effect-of-half-dose-and-standard-dose-conjugated-equine-estrogens-combined-with-natural-progesterone-or-dydrogesterone-on-components-of-metabolic-syndrome-in-healthy-postmenopausal-women-a-randomized-controlled-trial
#16
Wei Xue, Yan Deng, Yan-Fang Wang, Ai-Jun Sun
BACKGROUND: Menopausal hormone therapy (MHT) has been proven to have beneficial effects on several components of metabolic syndrome. However, the effects vary according to different regimens, dosages, and duration of MHT. The aim of the study was to evaluate the effect of standard-dose 0.625 mg conjugated equine estrogen (CEE) and half-dose 0.3 mg CEE daily with different progestogens in a continuous sequential regimen on postmenopausal metabolic parameters in generally healthy postmenopausal women...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27900293/combined-use-of-platelet-rich-plasma-micro-fat-in-sport-and-race-horses-with-degenerative-joint-disease-preliminary-clinical-study-in-eight-horses
#17
Fabrizio Bembo, Julia Eraud, Cecile Philandrianos, Baptiste Bertrand, Alain Silvestre, Julie Veran, Florence Sabatier, Guy Magalon, Jeremy Magalon
BACKGROUND: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). METHODS: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27820659/no-increase-in-fractures-after-stopping-hormone-therapy-results-from-the-women-s-health-initiative
#18
Nelson B Watts, Jane A Cauley, Rebecca D Jackson, Andrea Z LaCroix, Cora E Lewis, JoAnn E Manson, Joan Neuner, Lawrence S Phillips, Marcia L Stefanick, Jean Wactawski-Wende, Carolyn Crandall
CONTEXT: The Women's Health Initiative (WHI) hormone therapy (HT) trials showed protection against hip and total fractures but a later observational report suggested loss of benefit and a rebound increased risk after stopping. OBJECTIVE: To examine fractures after discontinuation of HT Design and Setting: Two placebo-controlled randomized trials Patients: 15,187 WHI participants who continued active HT or placebo through the intervention period and did not take HT in the post-intervention period Interventions: Conjugated equine estrogen + medroxyprogesterone acetate (CEE+MPA) in naturally menopausal women and conjugated equine estrogen (CEE) alone in women with prior hysterectomy Main Outcome Measures: Total and hip fractures through 5 years after discontinuation Results: Hip fractures were infrequent (∼2...
November 7, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27818457/isolation-and-characterization-of-equine-dental-pulp-stem-cells-derived-from-thoroughbred-wolf-teeth
#19
Shingo Ishikawa, Chie Horinouchi, Daiki Murata, Shota Matsuzaki, Kazuhiro Misumi, Yohei Iwamoto, Kenji Korosue, Seiji Hobo
Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth...
January 20, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27816621/degradation-intra-articular-retention-and-biocompatibility-of-monospheres-composed-of-pdlla-peg-pdlla-b-plla-multi-block-copolymers
#20
Maria J Sandker, Luisa F Duque, Everaldo M Redout, Alan Chan, Ivo Que, Clemens W G M Löwik, Evelien C Klijnstra, Nicole Kops, Rob Steendam, Rene van Weeren, Wim E Hennink, Harrie Weinans
: In this study, we investigated the use of microspheres with a narrow particle size distribution ('monospheres') composed of biodegradable poly(DL-lactide)-PEG-poly(DL-lactide)-b-poly(L-lactide) multiblock copolymers that are potentially suitable for local sustained drug release in articular joints. Monospheres with sizes of 5, 15 and 30μm and a narrow particle size distribution were prepared by a micro-sieve membrane emulsification process. During in vitro degradation, less crystallinity, higher swelling and accelerated mass loss during was observed with increasing the PEG content of the polymer...
November 2, 2016: Acta Biomaterialia
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