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https://www.readbyqxmd.com/read/30233367/gene-therapy-using-plasmid-dna-encoding-vegf164-and-fgf2-genes-a-novel-treatment-of-naturally-occurring-tendinitis-and-desmitis-in-horses
#1
Milomir Kovac, Yaroslav A Litvin, Ruslan O Aliev, Elena Y Zakirova, Catrin S Rutland, Andrey P Kiyasov, Albert A Rizvanov
This clinical study describes the intralesional application of the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor (VEGF164) and fibroblast growth factor 2 (FGF2) in seven horses to restore naturally occurring injuries of the superficial digital flexor tendon (SDFT) (tendinitis) and in three horses with suspensory ligament branch desmitis. Following application all horses were able to commence a more rapid exercise program in comparison to standardized exercise programs...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/30220956/equine-facilitated-therapy-for-complex-trauma-eft-ct
#2
Tiffany M Naste, Maggi Price, Jane Karol, Lia Martin, Kathryn Murphy, Jennifer Miguel, Joseph Spinazzola
Emerging research suggests that Equine Facilitated Psychotherapy (EFP) may be beneficial for traumatized youth. In addition, complex trauma (i.e., multiple and/or prolonged developmentally adverse traumatic events which are typically interpersonal in nature) treatment research is still growing and there is a need for the development and examination of novel treatments for youth with complex trauma histories. The current article describes a promising EFP model for this population called Equine Facilitated Therapy for Complex Trauma (EFT-CT)...
2018: Journal of Child & Adolescent Trauma
https://www.readbyqxmd.com/read/30218639/purification-of-equine-igg3-by-lectin-affinity-and-an-interaction-analysis-via-microscale-thermophoresis
#3
Salvatore G De-Simone, Hilton J Nascimento, Isis C Prado, Aniesse S Aguiar, Anibal R Melgarejo, Jorge L Pina, Patricia F Ferreira, David W Provance
The availability of purified antibodies is a prerequisite for many applications and the appropriate choice(s) for antibody-purification is crucial. Numerous methods have been developed for the purification of antibodies from different sources with affinity chromatography-based methods being the most extensively utilized. These methods are based on high specificity, easy reversibility and biological interactions between two molecules (e.g., between receptor and ligand or antibody and antigen). However, no simple techniques have yet been described to characterize and purify subclasses of immunoglobulins (Ig) from some animals of biotechnology importance such as equines, which are frequently used to produce biotherapeutic antibodies...
September 12, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30191152/two-multicenter-surveys-on-equine-back-pain-10-years-a-part
#4
Barbara Riccio, Claudia Fraschetto, Justine Villanueva, Federica Cantatore, Andrea Bertuglia
Despite back-pain being a common cause of poor performance in sport horses, a tailored diagnostic workflow and a consolidated therapeutic approach are currently lacking in equine medicine. The aim of the study was to assess the evolution in the veterinarian approach to diagnose and treat back-pain over a 10 years period. To investigate this topic, two surveys were addressed to equine veterinarians working in practice throughout Europe 10 years apart (2006 and 2016). The answers were organized in an Excel dataset and analyzed...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/30157228/evaluation-of-chromocystoscopy-in-the-diagnosis-of-cystitis-in-female-donkeys
#5
Naglaa A Abd El Kader, Haithem A Farghali, Ashraf M Abu-Seida, Noha Y Salem, Marwa S Khattab
Early detection of cystitis in equine is essential to improve the prognosis and outcome of therapy. However, the conventional white light endoscopy is not sufficiently accurate for this purpose. Hence, this study evaluated chromoendoscopy as a recent diagnostic tool for cystitis in female donkeys. For this purpose, 5 apparently normal donkeys (control group) and 5 female donkeys with cystitis (diseased group) were used. Physical and rectal examinations, urine analysis, white light cystoscopy, methylene blue-based chromoendoscopy and histopathology were performed in all animals...
2018: PloS One
https://www.readbyqxmd.com/read/30142655/-nutrition-of-horses-with-equine-pituitary-pars-intermedia-dysfunction-cushing-s-syndrome-treated-with-pergolid-a-field-study
#6
Ellen Kienzle, Theresa Bockhorni
OBJECTIVE AND AIM: The nutritional status of 36 patients with equine pituitary pars intermedia dysfunction (PPID) under pergolide treatment was investigated. ANIMALS, MATERIALS AND METHODS: The body condi tion score (BCS) and feeding were determined at the beginning of the study and after 60 and 120 days. Sampled blood for control of pergolid therapy were used for insulin and glucose measurement. A standardized questionnaire regarding the symptoms of the disease, including hypertrichosis and weight change, was completed by the owners...
August 2018: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/30138538/27-hydroxycholesterol-an-endogenous-serm-and-risk-of-fracture-in-postmenopausal-women-a-nested-case-cohort-study-in-the-women-s-health-initiative
#7
Po-Yin Chang, David Feldman, Marcia L Stefanick, Donald P McDonnell, Bonne M Thompson, Jeffrey G McDonald, Jennifer S Lee
27-hydroxycholesterol (27HC) is a purported, novel endogenous SERM. In animal models, 27HC has an anti-estrogen effect in bone, and 17β-estradiol mitigates this effect. 27HC in relation to fracture risk has not been investigated in humans. Depending on the level of bioavailable 17β-estradiol (bioE2), 27HC may increase fracture risk in postmenopausal women and modify the fracture risk reduction from menopausal hormone therapy (MHT). To test these a priori hypotheses, we conducted a nested case-cohort study of 868 postmenopausal women within the Women's Health Initiative Hormone Therapy trials (WHI-HT)...
August 23, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/30123294/current-status-of-canine-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-veterinary-medicine
#8
REVIEW
Tania Sultana, Soojung Lee, Hun-Young Yoon, Jeong Ik Lee
Stem cell therapy has prompted the expansion of veterinary medicine both experimentally and clinically, with the potential to contribute to contemporary treatment strategies for various diseases and conditions for which limited or no therapeutic options are presently available. Although the application of various types of stem cells, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), adipose tissue-derived mesenchymal stem cells (AT-MSCs), and umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs), has promising potential to improve the health of different species, it is crucial that the benefits and drawbacks are completely evaluated before use...
2018: Stem Cells International
https://www.readbyqxmd.com/read/30109139/symptomatic-spinal-epidural-lipomatosis-after-combined-hormonal-and-steroidal-palliative-therapy-of-prostate-cancer
#9
Amir Awwad, Renan E Ibrahem Adam, Chandni Patel, James D Thomas
Introduction: Spinal epidural lipomatosis (SLE) is an abnormal accumulation of unencapsulated fat. The association of chronic steroid therapy is described as well as obesity and Cushing's syndrome. SLE has a range of neurological presentations such as back pain, myelopathy, and cauda equina syndrome. Surgical decompression is known to improve symptomatic cases and prevent further deterioration. Case presentation: An elderly male patient with background history of high-risk metastatic prostate cancer and long-term treatment with steroids and hormonal therapy presented with acute on chronic back pain and neurological symptoms...
2018: Spinal Cord Series and Cases
https://www.readbyqxmd.com/read/30103915/practical-considerations-for-clinical-use-of-mesenchymal-stem-cells-from-the-laboratory-to-the-horse
#10
REVIEW
L Barrachina, A Romero, P Zaragoza, C Rodellar, F J Vázquez
Since the clinical use of mesenchymal stem cells (MSCs) for treating musculoskeletal injuries is gaining popularity, practitioners should be aware of the factors that may affect MSCs from tissue harvesting for MSC isolation to cell delivery into the injury site. This review provides equine practitioners with up-to-date, practical knowledge for the treatment of equine patients using MSCs. A brief overview of laboratory procedures affecting MSCs is provided, but the main focus is on shipping conditions, routes of administration, injection methods, and which commonly used products can be combined with MSCs and which products should be avoided as they have deleterious effects on cells...
August 2018: Veterinary Journal
https://www.readbyqxmd.com/read/30095195/effect-of-culture-duration-on-chondrogenic-preconditioning-of-equine-bone-marrow-mesenchymal-stem-cells-in-self-assembling-peptide-hydrogel
#11
John D Kisiday, Aimee C Colbath, Suwimol Tangtrongsup
Ex vivo induction of chondrogenesis is a promising approach to improve upon the use of bone marrow mesenchymal stem cells (MSCs) for cartilage tissue engineering. This study evaluated the potential to induce chondrogenesis with days of culture in chondrogenic medium for MSCs encapsulated in self-assembling peptide hydrogel. To simulate the transition from preconditioning culture to implantation, MSCs were isolated from self-assembling peptide hydrogel into an individual cell suspension. Commitment to chondrogenesis was evaluated by seeding preconditioned MSCs into agarose and culturing in the absence of the chondrogenic cytokine transforming growth factor beta (TGFβ)...
August 10, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/30089549/recent-advances-in-conservative-and-surgical-treatment-options-of-common-equine-foot-problems
#12
REVIEW
S D Gutierrez-Nibeyro, A M McCoy, K T Selberg
Foot problems are very common causes of lameness in horses. With the recent diagnostic advances to evaluate and treat foot pathology as well as to monitor response to therapy, it is now possible to more accurately evaluate the effectiveness of many of these treatments. This review details some of the recent advances of the most common conservative and surgical treatment options for foot problems in horses, including an overview of evidence on the efficacy to support the use of these treatment options and on factors that may affect prognosis...
July 2018: Veterinary Journal
https://www.readbyqxmd.com/read/30087008/plasminogen-activator-inhibitor-1-and-tenascin-c-secreted-by-equine-mesenchymal-stromal-cells-stimulate-dermal-fibroblast-migration-in-vitro-and-contribute-to-wound-healing-in-vivo
#13
Rebecca M Harman, Megan K He, Sheng Zhang, Gerlinde R VAN DE Walle
BACKGROUND: Impaired cutaneous wound healing is common in humans, and treatments are often ineffective. Based on the significant emotional and economic burden of impaired wound healing, innovative therapies are needed. The potential of mesenchymal stromal cell (MSC)-secreted factors to treat cutaneous wounds is an active area of research that is in need of refinement before effective clinical trials can be initiated. The aims of the present study were to (i) study which MSC-secreted factors stimulate dermal fibroblast (DF) migration in vitro and (ii) evaluate the potential of these factors to promote wound healing in vivo...
August 2018: Cytotherapy
https://www.readbyqxmd.com/read/30081016/racial-differences-in-the-effects-of-hormone-therapy-on-incident-open-angle-glaucoma-in-a-randomized-trial
#14
T S Vajaranant, R M Ray, L R Pasquale, J A Mares, R Ritch, E W Gower, M N Haan, R D Jackson, P M Maki
PURPOSE: We conducted a secondary analysis of a randomized, placebo-controlled trial to test if hormone therapy (HT) altered the risk of open-angle glaucoma (OAG), and if the risk reduction varied by race. DESIGN: Secondary analysis of randomized controlled trial data Methods: We linked Medicare claims data to 25,535 women in the Women's Health Initiative. Women without a uterus were randomized to receive either oral conjugated equine estrogens (CEE 0.625mg/day) or placebo, and women with a uterus received oral CEE and medroxyprogesterone acetate (CEE 0...
August 4, 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/30055679/transitioning-veterans-to-nursing-careers-a-model-program
#15
Patricia Voelpel, Lori Escallier, Judith Fullerton, Ismael Rodriguez
BACKGROUND: A commitment to increase the enrollment, retention and educational success of United States veterans admitted to a baccalaureate degree nursing program was established through the support of a grant received from HRSA in collaboration with the US Departments of Defense and Veteran Affairs. METHOD: Challenges encountered by the student veterans were identified and programs of mentorship, tutoring, equine therapy and interface with services offered by the University Office of Veteran Affairs were developed...
July 2018: Journal of Professional Nursing: Official Journal of the American Association of Colleges of Nursing
https://www.readbyqxmd.com/read/30047455/-an-educator-s-perspective-defusing-tensions-through-mediation-and-the-encounter
#16
Gérard Rivoire
Non-pharmacological alternatives which avoid or reduce the use of seclusion and restraint help to defuse psychological and/or physical tension to move towards appeasement. For the professional, the encounter with the patient requires questioning, reflection, availability as well as creativity. Mediation techniques such as hydrotherapy or equine assisted therapy, as well as the caregiver's general attitude, are perfect illustrations. Testimony.
July 2018: Soins. Psychiatrie
https://www.readbyqxmd.com/read/30046733/biomarkers-menopausal-hormone-therapy-and-risk-of-venous-thrombosis-the-women-s-health-initiative
#17
Mary Cushman, Joseph C Larson, Frits R Rosendaal, Susan R Heckbert, J David Curb, Lawrence S Phillips, Alison E Baird, Charles B Eaton, Randall S Stafford
<AbstractText Label="Background" NlmCategory="UNASSIGNED">Oral menopausal hormone therapy causes venous thrombosis but whether biomarkers of thrombosis risk can identify women at risk is unknown. Methods: We completed a nested case control study in the two Women's Health Initiative hormone trials; 27 347 women aged 50-79 were randomized to hormone therapy (conjugated equine estrogen with or without medroxyprogesterone acetate) or placebo. With 4 years follow-up, biomarkers were measured using stored baseline samples prior to starting treatment, and one-year later, in 215 women who developed thrombosis and 867 controls...
April 2018: Research and Practice in Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/30044182/comparison-of-antibacterial-and-immunological-properties-of-mesenchymal-stem-stromal-cells-from-equine-bone-marrow-endometrium-and-adipose-tissue
#18
Yennifer Cortés-Araya, Karin Amilon, Burgunde Elisabeth Rink, Georgina Black, Zofia Lisowski, Francesc Xavier Donadeu, Cristina L Esteves
Equine mesenchymal stem/stromal cells (MSCs) are multipotent cells that are widely used for treatment of musculoskeletal injuries, and there is significant interest in expanding their application to nonorthopedic conditions. MSCs possess antibacterial and immunomodulatory properties that may be relevant for combating infection; however, comparative studies using MSCs from different origins have not been carried out in the horse, and this was the focus of this study. Our results showed that MSC-conditioned media attenuated the growth of Escherichia coli, and that this effect was, on average, more pronounced for endometrium (EM)-derived and adipose tissue (AT)-derived MSCs than for bone marrow (BM)-derived MSCs...
September 6, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/30026642/does-antimicrobial-therapy-improve-outcomes-in-horses-with-severe-equine-asthma-and-a-positive-tracheal-wash-bacterial-culture
#19
Michelle L Husulak, Stephen T Manning, Melissa D Meachem, Hilary J Burgess, Tasha Y Epp, Julia B Montgomery
The objective of this study was to observe the outcomes of adding an antimicrobial treatment to a conventional treatment regime in horses with severe equine asthma in a clinical setting. Eleven client-owned horses with a history consistent with severe equine asthma, increased respiratory effort and nostril flaring, ≥ 20% neutrophils on bronchoalveolar lavage (BAL), and a positive tracheal wash (TW) bacterial culture were treated with environmental management, corticosteroids, and bronchodilators. Six horses were also treated with an antimicrobial (principal group), while the other 5 were administered saline as a placebo (control group)...
July 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/30026624/internal-neurolysis-of-the-maxillary-branch-of-the-trigeminal-nerve-for-the-treatment-of-equine-trigeminal-mediated-headshaking-syndrome
#20
Chris Bell, Luke Hnenny, Kris Torske
A 5-year-old Hannovarian warmblood gelding was presented for recurrent headshaking exacerbated with exercise. The horse displayed clinical signs of repetitive vertical head movements, face rubbing on the forelimbs and on the ground, repetitive sneezing, and striking the muzzle with his forelimbs. The clinical signs resulted in a horse that could not be ridden and was dangerous. Clinical signs were most persistent in direct sunlight, but occurred with excitement, exercise, or bridling indoors. A diagnosis of equine trigeminal mediated headshaking syndrome was made...
July 2018: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
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