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https://www.readbyqxmd.com/read/30389141/slit3-regulates-endochondral-ossification-by-%C3%AE-catenin-suppression-in-chondrocytes
#1
Hanjun Kim, Young-Jin Choi, Young-Sun Lee, Suk Young Park, Ji-Eun Baek, Ho-Kyoung Kim, Beom-Jun Kim, Seung Hun Lee, Jung-Min Koh
Previously, we noted that SLIT3, slit guidance ligand 3, had an osteoprotective role with bone formation stimulation and bone resorption suppression. Additionally, we found that global Slit3 KO mice had smaller long bone. Skeletal staining showed short mineralized length in the newborn KO mice and wide hypertrophic chondrocyte area in the embryo KO mice, suggesting delayed chondrocyte maturation. The recombinant SLIT3 did not cause any change in proliferation of ATDC5 cells, but stimulated expressions of chondrocyte differentiation markers, such as COL2A1, SOX9, COL10A1, VEGF, and MMP13 in the cells...
October 31, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30282427/effects-of-three-dimensionally-printed-polycaprolactone-%C3%AE-tricalcium-phosphate-scaffold-on-osteogenic-differentiation-of-adipose-tissue-and-bone-marrow-derived-stem-cells
#2
Hannara Park, Jin Soo Kim, Eun Jung Oh, Tae Jung Kim, Hyun Mi Kim, Jin Hyung Shim, Won Soo Yoon, Jung Bo Huh, Sung Hwan Moon, Seong Soo Kang, Ho Yun Chung
BACKGROUND: Autogenous bone grafts have several limitations including donor-site problems and insufficient bone volume. To address these limitations, research on bone regeneration is being conducted actively. In this study, we investigate the effects of a three-dimensionally (3D) printed polycaprolactone (PCL)/tricalcium phosphate (TCP) scaffold on the osteogenic differentiation potential of adipose tissue-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMSCs). METHODS: We investigated the extent of osteogenic differentiation on the first and tenth day and fourth week after cell culture...
September 2018: Archives of Craniofacial Surgery
https://www.readbyqxmd.com/read/30215496/-effectiveness-comparison-between-the-paratricipital-approach-and-the-chevron-olecranon-v-osteotomy-approach-in-the-treatment-of-type-c3-distal-humeral-fractures
#3
Chuan Zhang, Zuojun Zhang, Zhongxiao Chang, Pinglin Yang, Ming Zhao, Xingxing Li, Guojie Wang, Xiaobo Duan
Objective: To compare the effectiveness between paratricipital approach and chevron olecranon V osteotomy approach for the treatment of type C3 (AO/OTA) distal humeral fractures and investigate the details of operation. Methods: Between April 2010 and September 2016, 36 type C3 (AO/OTA) distal humeral fractures were treated with open reduction and bicolumnar orthogonal locking plating fixation by paratricipital approach and chevron olecranon V osteotomy approach respectively...
October 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/30156864/mir-497-is-implicated-in-the-process-of-chondrogenesis-and-inhibits-ihh-gene-expression-in-human-chondrocytes
#4
Rui Zhang, Fei Cong, Qi Li, Zixin Min, Jidong Yan, Qian Zhang, Jie Ma, Shemin Lu, Jianbing Ma
Objective The aim of this study was to examine differences in microRNA-497 (miR-497) expression during cartilage tissue formation and to test whether miR-497 directly interferes with Indian hedgehog (IHH) gene and inhibits IHH expression in human chondrocytes. Design At different cartilage development stages and different time points in bone matrix gelatin-induced endochondral ossification (BMG-ECO) rat models, the expression of miR-497 and the Ihh gene was monitored at the mRNA level. Bioinformatic analysis, gene mutation, dual luciferase reporter gene assays and gene expression assays at both the mRNA and protein levels in human chondrocytes were subsequently performed to validate the interaction between miR-497 and the IHH gene...
August 29, 2018: Cartilage
https://www.readbyqxmd.com/read/30032135/mutant-mapk7-induced-idiopathic-scoliosis-is-linked-to-impaired-osteogenesis
#5
Taifeng Zhou, Chong Chen, Caixia Xu, Hang Zhou, Bo Gao, Deying Su, Zhiheng Liao, Yongyong Li, Shulan Yang, Peiqiang Su
BACKGROUND/AIMS: Three rare MAPK7 variants that predispose individuals to adolescent idiopathic scoliosis have previously been identified. However, the mechanism underlying the effects of the mutations remain unknown. METHODS: Human mesenchymal stem cells (hMSCs) were isolated from both patients and healthy volunteer donors, and MAPK7 expression was detected by western blotting and real-time quantitative PCR (RT-qPCR). Zebrafish embryos were injected with mapk7 morpholinos or co-injected with morpholinos and wild-type (WT) MAPK7 messenger RNA (mRNA) at the one-cell stage, followed by calcein staining to evaluate bone formation...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29988728/a-novel-role-in-skeletal-segment-regeneration-of-extracellular-vesicles-released-from-periodontal-ligament-stem-cells
#6
Francesca Diomede, Marco D'Aurora, Agnese Gugliandolo, Ilaria Merciaro, Valeria Ettorre, Alessia Bramanti, Adriano Piattelli, Valentina Gatta, Emanuela Mazzon, Antonella Fontana, Oriana Trubiani
Purpose: The combination of oral derived stem cells and 3-D scaffolds is considered advantageous in bone repair. In particular, collagen membranes possess ideal biological properties and can support infiltration and proliferation of osteoblasts, promoting bone regeneration. Our study aimed to develop a new biocompatible osteogenic construct composed of a commercially available collagen membrane (Evolution [Evo]), human periodontal-ligament stem cells (hPDLSCs) enriched with extracellular vesicles (EVs), or polyethylenimine (PEI)-engineered EVs (PEI-EVs)...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29913030/lateralized-center-of-rotation-and-lower-neck-shaft-angle-are-associated-with-lower-rates-of-scapular-notching-and-heterotopic-ossification-and-improved-pain-for-reverse-shoulder-arthroplasty-at-1-year
#7
Ryan Nelson, Jeremiah T Lowe, Sarah M Lawler, Megan Fitzgerald, Matthew T Mantell, Andrew Jawa
Heterotopic ossification and scapular notching are common following reverse total shoulder arthroplasty. Compared with the original Grammont-style prosthesis with a medialized center of rotation (COR) and a 155° neck-shaft angle, lateralization of COR and reduction of neck-shaft angle have been associated with decreased incidence of scapular notching. The authors hypothesized that these design features may also be effective in reducing heterotopic ossification after reverse total shoulder arthroplasty. Ninety-seven consecutive patients who underwent reverse total shoulder arthroplasty performed by a single surgeon were included in the study...
July 1, 2018: Orthopedics
https://www.readbyqxmd.com/read/29762846/neurotrophin-3-improves-fracture-healing-in-rats
#8
X Li, D-C Sun, Y Li, X-Y Wu
OBJECTIVE: This study aimed at investigating the role of Neurotrophin-3 (NT-3) in the bone fracture healing of rats and to provide a reference for clinical treatment. MATERIALS AND METHODS: 40 Sprague-Dawley (SD) rats were randomly divided into control group and NT-3 group. The tibia fracture model was made in NT-3 group, and the tibia bone mineral density (BMD) was measured before and after the surgery. The biomechanics indexes were inspected after the surgery, including elasticity modulus, max load, and bending rigidity...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29713041/rna-sequencing-of-xp11-translocation-associated-cancers-reveals-novel-gene-fusions-and-distinctive-clinicopathologic-correlations
#9
Xiao-Tong Wang, Qiu-Yuan Xia, Sheng-Bing Ye, Xuan Wang, Rui Li, Ru Fang, Shan-Shan Shi, Ru-Song Zhang, Xiao Tan, Jie-Yu Chen, Ke Sun, Xiao-Dong Teng, Heng-Hui Ma, Zhen-Feng Lu, Xiao-Jun Zhou, Qiu Rao
Both Xp11 translocation renal cell carcinomas and the corresponding mesenchymal neoplasms are characterized by a variety of gene fusions involving TFE3. It has been known that tumors with different gene fusions may have different clinicopathologic features; however, further in-depth investigations of subtyping Xp11 translocation-associated cancers are needed in order to explore more meaningful clinicopathologic correlations. A total of 22 unusual cases of Xp11 translocation-associated cancers were selected for the current study; 20 cases were further analyzed by RNA sequencing to explore their TFE3 gene fusion partners...
April 30, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29616218/recent-insights-into-the-contribution-of-the-changing-hypertrophic-chondrocyte-phenotype-in-the-development-and-progression-of-osteoarthritis
#10
REVIEW
Ellen G J Ripmeester, Ufuk Tan Timur, Marjolein M J Caron, Tim J M Welting
Osteoarthritis (OA) is an extremely prevalent age-related condition. The economic and societal burden due to the cost of symptomatic treatment, inability to work, joint replacement, and rehabilitation is huge and increasing. Currently, there are no effective medical therapies that delay or reverse the pathological manifestations of OA. Current treatment options are, without exception, focused on slowing down progression of the disease to postpone total joint replacement surgery for as long as possible and keeping the associated pain and joint immobility manageable...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29545873/semaphorin-3a-promotes-osteogenic-differentiation-in-human-alveolar-bone-marrow-mesenchymal-stem-cells
#11
Li Liu, Jue Wang, Xiaomeng Song, Qingping Zhu, Shuping Shen, Wei Zhang
The aim of the present study was to investigate the role of Semaphorin 3A (Sema3A) in the osteogenic differentiation of human alveolar bone marrow mesenchymal stem cells (hABMMSCs). To investigate whether Sema3A affects hABMMSC proliferation and osteogenic differentiation, a stable Sema3A-overexpression cell line was generated by infection with the pAdCMV-SEMA3A-MCS-EGFP vector. Cell counting kit-8 and clone formation assays were performed to determine the proliferation ability of hABMMSCs, while cell osteogenic differentiation was assayed using Alizarin Red S staining...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29381614/bone-conditioned-medium-obtained-from-calvaria-mandible-and-tibia-cause-an-equivalent-tgf-%C3%AE-1-response-in-vitro
#12
Babak Hatami Kia, João Rui Galvão Mendes, Heinz-Dieter Müller, Patrick Heimel, Reinhard Gruber
Bones with different embryological origin and mode of ossification are supposed to vary in their capacity for supporting graft consolidation. The aim of the current pilot study was to assess the TGF-β1 activity of bone chips obtained from distinct anatomic locations. Conditioned medium was prepared from bone chips harvested from pig calvaria, mandible, and tibia. Human oral fibroblasts were exposed to bone-conditioned medium (BCM) followed by reverse transcriptase polymerase chain reaction of the TGF-β1 target genes...
May 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29342370/fgf8-signaling-alters-the-osteogenic-cell-fate-in-the-hard-palate
#13
J Xu, Z Huang, W Wang, X Tan, H Li, Y Zhang, W Tian, T Hu, Y P Chen
Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulate the osteogenic cell fate in the developing palate remain unclear. In the present study, we investigated the potential role of FGF signaling in osteogenic fate determination of the palatal mesenchymal cells...
May 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29314250/inhibition-of-camkk2-enhances-fracture-healing-by-stimulating-indian-hedgehog-signaling-and-accelerating-endochondral-ossification
#14
Justin N Williams, Anuradha Valiya Kambrath, Roshni B Patel, Kyung Shin Kang, Elsa Mével, Yong Li, Ying-Hua Cheng, Austin J Pucylowski, Mariah A Hassert, Michael J Voor, Melissa A Kacena, William R Thompson, Stuart J Warden, David B Burr, Matthew R Allen, Alexander G Robling, Uma Sankar
Approximately 10% of all bone fractures do not heal, resulting in patient morbidity and healthcare costs. However, no pharmacological treatments are currently available to promote efficient bone healing. Inhibition of Ca2+ /calmodulin (CaM)-dependent protein kinase kinase 2 (CaMKK2) reverses age-associated loss of trabecular and cortical bone volume and strength in mice. In the current study, we investigated the role of CaMKK2 in bone fracture healing and show that its pharmacological inhibition using STO-609 accelerates early cellular and molecular events associated with endochondral ossification, resulting in a more rapid and efficient healing of the fracture...
May 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29290604/grammont-humeral-design-versus-onlay-curved-stem-reverse-shoulder-arthroplasty-comparison-of-clinical-and-radiographic-outcomes-with-minimum-2-year-follow-up
#15
COMPARATIVE STUDY
Giovanni Merolla, Gilles Walch, Francesco Ascione, Paolo Paladini, Elisabetta Fabbri, Antonio Padolino, Giuseppe Porcellini
BACKGROUND: There are few investigations comparing lateralized and medialized reverse total shoulder arthroplasty (RTSA) in patients with cuff tear arthropathy. This study assessed the outcomes of 2 RTSA designs. METHODS: Sixty-eight consecutive cuff tear arthropathy patients (74 shoulders) with a follow-up of at least 24 months received a Grammont or an onlay curved short-stem humeral component, with or without glenoid lateralization; a cementless humeral stem was implanted in >90%...
April 2018: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29207198/mechanism-of-triptolide-in-treating-ankylosing-spondylitis-through-the-anti%C3%A2-ossification-effect-of-the-bmp-smad-signaling-pathway
#16
Guilong Wang, Jing Cai, Jinshan Zhang, Cuiyun Li
The present study aimed to examine the mechanism of triptolide in the treatment of ankylosing spondylitis (AS) through the anti‑ossification effect of bone morphogenetic protein (BMP)/small mothers against decapentaplegic (Smad) pathway. Male rats were randomly divided into five groups: Normal rat group; model group; triptolide low dose group (10 mg/kg); triptolide middle dose group (20 mg/kg); triptolide high dose group (40 mg/kg). The spinal joint capsules of each group of rats were collected to perform primary cell culture to determine the levels of cell proliferation...
February 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29191939/effects-of-hypothalamic-leptin-gene-therapy-on-osteopetrosis-in-leptin-deficient-mice
#17
Kenneth A Philbrick, Stephen A Martin, Amy R Colagiovanni, Adam J Branscum, Russell T Turner, Urszula T Iwaniec
Impaired resorption of cartilage matrix deposited during endochondral ossification is a defining feature of juvenile osteopetrosis. Growing, leptin-deficient ob/ob mice exhibit a mild form of osteopetrosis. However, the extent to which the disease is (1) self-limiting and (2) reversible by leptin treatment is unknown. We addressed the first question by performing histomorphometric analysis of femurs in rapidly growing (2-month-old), slowly growing (4-month-old) and skeletally mature (6-month-old) wild-type (WT) and ob/ob male mice...
February 2018: Journal of Endocrinology
https://www.readbyqxmd.com/read/29072695/glucocorticoid-mediates-prenatal-caffeine-exposure-induced-endochondral-ossification-retardation-and-its-molecular-mechanism-in-female-fetal-rats
#18
Yangfan Shangguan, Hongqiang Jiang, Zhengqi Pan, Hao Xiao, Yang Tan, Kai Tie, Jun Qin, Yu Deng, Liaobin Chen, Hui Wang
Our previous studies discovered that prenatal caffeine exposure (PCE) could induce intrauterine growth retardation (IUGR) and long-bone dysplasia in offspring rats, accompanied by maternal glucocorticoid over-exposure. This study is to explore whether intrauterine high glucocorticoid level can cause endochondral ossification retardation and clarify its molecular mechanism in PCE fetal rats. Pregnant Wistar rats were intragastrically administered 30 and 120 mg/kg day of caffeine during gestational days (GDs) 9-20, then collected fetal serum and femurs at GD20...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28969891/standard-versus-bony-increased-offset-reverse-shoulder-arthroplasty-a-retrospective-comparative-cohort-study
#19
COMPARATIVE STUDY
Philippe Collin, Xin Liu, Patrick J Denard, Soleen Gain, Alexandra Nowak, Alexandre Lädermann
BACKGROUND: To date, only a few comparative studies with small sample sizes have compared a traditional reverse shoulder arthroplasty (tRSA) to a bony increased-offset RSA (BIO-RSA). We hypothesized that the BIO-RSA would lead to lower notching rates and improved range of motion (ROM) compared with a tRSA. METHODS: A retrospective review was performed of 69 tRSAs and 61 BIO-RSAs performed by a single surgeon. At 2 years postoperative, ROM and Constant scores were compared...
January 2018: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28966669/salvianolic-acid-b-and-danshensu-induce-osteogenic-differentiation-of-rat-bone-marrow-stromal-stem-cells-by-upregulating-the-nitric-oxide-pathway
#20
Xinle Zhang, Liyi Zou, Jin Li, Bilian Xu, Tie Wu, Huanqiong Fan, Weiming Xu, Weimin Yao, Yajun Yang, Yuyu Liu, Liao Cui
The aim of the present study was to investigate the effect of salvianolic acid B (Sal B) and danshensu (DSU) on the osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) and the mechanisms of the effects. The osteogenic differentiation of MSCs in culture was assessed by measuring alkaline phosphatase (ALP) activity, osteocalcin (OCN) production, nitric oxide (NO) production and the mRNA expression levels of osteoprotegerin (OPG) and its ligand by MSCs. MSCs were successfully induced to differentiate into osteoblasts and adipocytes...
October 2017: Experimental and Therapeutic Medicine
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