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reverse ossification

Ying Shao, Qian-Zhao Chen, Yu-Hua Zeng, Yang Li, Wen-Yan Ren, Lin-Yun Zhou, Rong-Xin Liu, Ke Wu, Jun-Qing Yang, Zhong-Liang Deng, Yu Yu, Wen-Juan Sun, Bai-Cheng He
Rosiglitazone (RSG) is a potent drug used in the treatment of insulin resistance; however, it is associated with marked skeletal toxicity. RSG-induced osteoporosis may contribute to the promotion of adipogenic differentiation at the expense of osteogenic differentiation in bone marrow stromal cells. The aim of this study was to investigate whether RSG-induced bone toxicity can be reversed by combined treatment with all-trans retinoic acid (ATRA). We examined different osteogenic markers in mouse embryonic fibroblasts (MEFs) following treatment with RSG, ATRA, or RSG and ATRA in combination...
October 19, 2016: International Journal of Molecular Medicine
Kai Hu, Tatiana Y Besschetnova, Bjorn R Olsen
BMP2 is widely used for promotion of bone repair and regeneration. However, bone formation induced by BMP2 is quite variable. Bone forming progenitor cells in different locations appear to respond to BMP2 in different ways, and repair outcomes can vary as a consequence of modulating effects by other factors. In this study, we have examined the effects of VEGF on BMP2-induced repair of a cortical bone defect, a 1 mm diameter drill hole, in the proximal tibia of mice. Treatment of the defect with either a bolus of PBS or soluble VEGFR1 (sVEGFR1), a decoy receptor for VEGF, had the same effects on bone formation via intramembranous ossification in the defect and cartilage formation and injured periosteum, during the healing process...
September 7, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
B Verhofste, T Decock, A Van Tongel, L De Wilde
AIMS: Heterotopic ossification (HO) occurs after arthroplasty, especially total hip arthroplasty. In this study we describe the incidence, evolution, morphology and clinical consequences of HO following reverse shoulder arthroplasty. PATIENTS AND METHODS: This is a single-centre retrospective study of 132 consecutive patients who received a Delta III or Delta Xtend reverse total shoulder arthroplasty between 2006 and 2013 for the treatment of cuff tear arthropathy...
September 2016: Bone & Joint Journal
Jordan J Allensworth, Karla D O'Dell, Joshua S Schindler
BACKGROUND: Diffuse idiopathic skeletal hyperostosis (DISH) is an idiopathic spinal disease common in the elderly and characterized by flowing ossification and osteophyte formation along the spinal column. Cervical hyperostosis is capable of producing dysphagia, stridor, and airway obstruction; however, there are no extant reports of true paralysis of bilateral vocal folds in patients fulfilling the criteria for DISH. METHODS AND RESULTS: We report a case of a 61-year-old man presenting with dysphagia and dyspnea...
August 24, 2016: Head & Neck
Chong Chen, Caixia Xu, Taifeng Zhou, Bo Gao, Hang Zhou, Changhua Chen, Changli Zhang, Dongsheng Huang, Peiqiang Su
Abnormalities of membranous and endochondral ossification in patients with adolescent idiopathic scoliosis (AIS) remain incompletely understood. To investigate abnormalities in the melatonin signaling pathway and cellular response to melatonin in AIS, a case‑control study of osteogenic and chondrogenic differentiation was performed using human mesenchymal stem cells (hMSCs). AIS was diagnosed by physical and radiographic examination. hMSCs were isolated from the bone marrow of patients with AIS and control subjects (n=12 each), and purified by density gradient centrifugation...
August 2016: Molecular Medicine Reports
Jia-Wei Kevin Ko, Jeffrey D Tompson, Daniel S Sholder, Eric M Black, Joseph A Abboud
BACKGROUND: Heterotopic ossification (HO) around shoulder arthroplasty is a frequent finding with unclear clinical relevance. This study evaluated the incidence, relevance, and predisposing factors of HO in the long head of the triceps tendon after reverse shoulder arthroplasty. METHODS: Retrospective chart review was conducted to identify patients who had a reverse shoulder arthroplasty performed between 2008 and 2012. Patient demographics, implant types, and diagnoses were noted...
June 1, 2016: Journal of Shoulder and Elbow Surgery
Yu Chen, Haisong Yang, Jinhao Miao, Xiaowei Liu, Xinwei Wang, Deyu Chen
STUDY DESIGN: Vitro molecular research on the posterior longitudinal ligament fibroblasts. OBJECTIVE: To investigate different expression of OASIS between spinal ligament fibroblasts from the patients with ossification of the posterior longitudinal ligament (OPLL) and non-OPLL patients and demonstrate knockdown of OASIS protein expression by RNA interference inhibiting expression of type I collagen in OPLL cells. SUMMARY OF BACKGROUND DATA: OPLL is characterized by ectopic bone formation in spinal ligaments...
May 11, 2016: Clinical Spine Surgery
Vaida Glatt, Nicole Bartnikowski, Nicholas Quirk, Michael Schuetz, Christopher Evans
BACKGROUND: Reverse dynamization is a technology for enhancing the healing of osseous defects. With use of an external fixator, the axial stiffness across the defect is initially set low and subsequently increased. The purpose of the study described in this paper was to explore the efficacy of reverse dynamization under different conditions. METHODS: Rat femoral defects were stabilized with external fixators that allowed the stiffness to be modulated on living animals...
April 20, 2016: Journal of Bone and Joint Surgery. American Volume
L Obert, R Saadnia, C Tournier, N Bonnevialle, D Saragaglia, F Sirveaux
INTRODUCTION: The reversed shoulder prosthesis is becoming the gold standard for cases of complex fracture of the proximal humerus after 70 years of age. MATERIAL AND METHODS: The French Society of Orthopaedic and Traumatology Surgery (SOFCOT) conducted a prospective and retrospective multicenter study to evaluate the results of the reversed shoulder prosthesis implanted in patients with a four-part fracture in nine centers. In the retrospective study (n=41 patients, 78 years of age, 14% ASA grade 3, 21% associated fractures) and in the prospective study (n=32 patients, 79 years of age, 37% ASA grade 3, 21% associated fractures) evaluation by independent surgeons was conducted to measure the QuickDASH score, the Constant score, the SSV (subjective shoulder value), and complications to correlate these measurements with radiological results...
May 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
Jian-Min Liu, Ya-Zhou Cui, Geng-Lin Zhang, Xiao-Yan Zhou, Jing-Xiang Pang, Xue-Zheng Wang, Jin-Xiang Han
BACKGROUND: Ankylosing spondylitis (AS) is the most common rheumatic condition that is slowly progressive and predominantly affects adolescents. Pathological bone formation associated with AS is an important cause of disability. The aim of the study was to investigate the possible involvement of the genes related to endochondral ossification and ectopia ossification in genetic susceptibility to AS in a Chinese Han population. METHODS: Sixty-eight single nucleotide polymorphisms (SNPs) from 13 genes were genotyped in discovery cohorts including 300 AS patients and 180 healthy controls...
March 20, 2016: Chinese Medical Journal
Joseph M Statz, Eric R Wagner, Matthew T Houdek, Robert H Cofield, Joaquin Sanchez-Sotelo, Bassem T Elhassan, John W Sperling
BACKGROUND: Obesity is a risk factor for worse outcomes in anatomic shoulder arthroplasty. The purpose of this investigation was to determine outcomes of primary reverse shoulder arthroplasty (RSA) in patients with morbid obesity. METHODS: We reviewed all primary RSAs performed on morbidly obese (body mass index [BMI] ≥40 kg/m(2)) patients from 2005 to 2012 at our institution with at least 2 years of follow-up. The study included 41 patients (57% female), the mean BMI was 44 kg/m(2), and the mean age was 67...
July 2016: Journal of Shoulder and Elbow Surgery
Ma Wei Juan, Guo Xiong, Umer Farooq
BACKGROUND: Kashin-Beck Disease (KBD) is a chronic deforming osteoarthritis starting in early childhood and affecting the cartilage metabolism and endochondral ossification. Selenium (Se) deficiency has been postulated as the major environmental etiological factor for KBD by many studies. Other minerals such as the Manganese (Mn) and calcium (Ca) which don't have uniform distribution in environment are also important elements involved in bone and cartilage formation but their regulation in KBD has been rarely reported...
July 2015: Journal of Ayub Medical College, Abbottabad: JAMC
K A Staines, K Madi, S M Mirczuk, S Parker, A Burleigh, B Poulet, M Hopkinson, A J Bodey, R C Fowkes, C Farquharson, P D Lee, A A Pitsillides
OBJECTIVE: To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they are attributable to an endochondral growth defect. METHODS: Knee joints from STR/Ort mice with advanced OA and age-matched CBA (control) mice were examined by Affymetrix microarray profiling, multiplex polymerase chain reaction (PCR) analysis, and immunohistochemical labeling of endochondral markers, including sclerostin and MEPE...
April 2016: Arthritis & Rheumatology
Luis Ossa-Fuentes, Jorge Mpodozis, Alexander O Vargas
The anklebone (astragalus) of dinosaurs presents a characteristic upward projection, the 'ascending process' (ASC). The ASC is present in modern birds, but develops a separate ossification centre, and projects from the calcaneum in most species. These differences have been argued to make it non-comparable to dinosaurs. We studied ASC development in six different orders of birds using traditional techniques and spin-disc microscopy for whole-mount immunofluorescence. Unexpectedly, we found the ASC derives from the embryonic intermedium, an ancient element of the tetrapod ankle...
2015: Nature Communications
Mirte Peeters, Suzanne E L Detiger, Lindsay S Karfeld-Sulzer, Theo H Smit, Avner Yayon, Franz E Weber, Marco N Helder
Intervertebral disc (IVD) degeneration is etiologically associated with low back pain and is currently only treated in severe cases with spinal fusion. Regenerative medicine attempts to restore degenerated tissue by means of cells, hydrogels, and/or growth factors and can therefore be used to slow, halt, or reverse the degeneration of the IVD in a minimally invasive manner. Previously, the growth factors bone morphogenetic proteins 2 and 7 (BMP-2, -7) were shown to enhance disc regeneration, in vitro and in vivo...
2015: BioResearch Open Access
Yu-Chen Lee, Song-Chang Lin, Guoyu Yu, Chien-Jui Cheng, Bin Liu, Hsuan-Chen Liu, David H Hawke, Nila U Parikh, Andreas Varkaris, Paul Corn, Christopher Logothetis, Robert L Satcher, Li-Yuan Yu-Lee, Gary E Gallick, Sue-Hwa Lin
Resistance to currently available targeted therapies significantly hampers the survival of patients with prostate cancer with bone metastasis. Here we demonstrate an important resistance mechanism initiated from tumor-induced bone. Studies using an osteogenic patient-derived xenograft, MDA-PCa-118b, revealed that tumor cells resistant to cabozantinib, a Met and VEGFR-2 inhibitor, reside in a "resistance niche" adjacent to prostate cancer-induced bone. We performed secretome analysis of the conditioned medium from tumor-induced bone to identify proteins (termed "osteocrines") found within this resistance niche...
November 15, 2015: Cancer Research
Y Xiong, S Wu, Q Du, A Wang, Z Wang
Cytogenetic analyses have revealed that complex karyotypes with numerous and highly variable genomic aberrations including single-nucleotide polymorphisms (SNPs) and copy number variants (CNVs), are observed in most of the conventional osteosarcomas (OSs). Several genome-wide studies have reported that the dysregulated expression of many genes is correlated with genomic aberrations in OS. We first compared OS gene expression in Gene Expression Omnibus (GEO) data sets and genomic aberrations in International Cancer Genome Consortium (ICGC) database to identify differentially expressed genes (DEGs) associated with SNPs or CNVs in OS...
November 2015: Cancer Gene Therapy
Dechun Chen, Yang Liu, Haisong Yang, Deyu Chen, Xiaoling Zhang, Julio C Fermandes, Yu Chen
Although cervical ossification of the posterior longitudinal ligament (OPLL) is one of the most common spinal diseases, the pathogenic mechanism is still not fully understood. Abnormal mechanical stress distribution is believed to be one of the main causes of OPLL. We have previously found that mechanical stress can up-regulate connexin 43 (Cx43) expression in ligament fibroblasts; this transduces mechanical signals to promote osteoblastic differentiation. In the present study, in order to explore further the intracellular mechanisms of Cx43-induced osteoblast differentiation of ligament fibroblasts, we investigate the potential roles of the osteogenic signaling pathway components ERK1/2, p38 MAPK and JNK in Cx43-mediated mechanical signal transduction...
March 2016: Cell and Tissue Research
Qian Huang, Liying Hao, Jing Xie, Tao Gong, Jinfeng Liao, Yunfeng Lin
In this study, water-soluble, one-step highly reduced and functionalized graphene oxide was prepared via a facile, environment-friendly method by using tea polyphenol (TP), which acted as both reducing agent and stabilizer. The product obtained, that is, tea polyphenol-reduced graphene oxide (TPG), was used as a reinforcing building block for the modification of a mechanically weak chitosan (CS), TPG/CS. The morphology and physicochemical and mechanical properties of the composite were examined by various characterizations...
September 23, 2015: ACS Applied Materials & Interfaces
Hironori Arima, Shinichi Sakamoto, Kentaro Naito, Toru Yamagata, Takehiro Uda, Kenji Ohata, Toshihiro Takami
BACKGROUND: The clinical significance of diffusion tensor (DT) magnetic resonance imaging (MRI) parameters was analyzed to predict postoperative functional recovery in patients with cervical myelopathy. MATERIALS AND METHODS: Sixteen patients with cervical myelopathy caused by cervical spondylosis, disk herniation or ossification of the posterior longitudinal ligament who underwent surgical intervention in our institute were enrolled in this retrospective study...
July 2015: Journal of Craniovertebral Junction and Spine
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