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reverse ossification

Xiaomin Kang, Wei Yang, Dongxu Feng, Xinxin Jin, Zhengmin Ma, Zhuang Qian, Tianping Xie, Huixia Li, Jiali Liu, Ruiqi Wang, Fang Li, Danhui Li, Hongzhi Sun, Shufang Wu
Autophagy is activated during nutritionally depleted or hypoxic conditions to facilitate cell survival. Because growth plate is an avascular and hypoxic tissue, autophagy may have a crucial role during chondrogenesis; however, the functional role and underlying mechanism of autophagy in regulation of growth plate remains elusive. In this study, we generated (TamCart) Atg7(-/-) (Atg7cKO) mice to explore the role of autophagy during endochondral ossification. Atg7cKO mice exhibited growth retardation associated with reduced chondrocyte proliferation and differentiation, and increased chondrocyte apoptosis...
March 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
A Tekari, R D May, D A Frauchiger, S C Chan, L M Benneker, B Gantenbein
Spinal fusion is hampered by the presence of remaining intervertebral disc (IVD) tissue and leads to spinal non-union. While the exact mechanism remains unknown, we hypothesise that factors preventing disc ossification, such as antagonists of the bone morphogenetic proteins (BMP), could be responsible for this process. The objective of this study was to investigate spinal non-union using an in vitro human model with a focus on the BMP signalling components and to identify factors contributing to the incomplete and delayed ossification...
February 23, 2017: European Cells & Materials
Wei-Zhe Shi, Jin-Yong Ju, Hai-Jun Xiao, Feng Xue, Jiang Wu, Ming-Mang Pan, Wei-Feng Ni
The present study aimed to detect early changes in the concentration of matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase‑2 (MMP‑2) and tissue inhibitor of metalloproteinase‑1 (TIMP‑1) in a rat model of brain injury combined with traumatic heterotopic ossification (HO). A total of 132 male Sprague‑Dawley rats were used to establish the experimental and control groups. Anatomy and sample collection were conducted on postoperative days 1, 2, 3, 4, 5, 6 and 7. Hematoxylin and eosin and immunohistochemical staining were performed for local tissues...
April 2017: Molecular Medicine Reports
Konstantinos Stefanidis, Peter Brindley, Raymond Ramnarine, Michael Blaivas, Mohammad Daneshi, Paul S Sidhu, Abdoulrahman Alharthy, Dimitrios Karakitsos
OBJECTIVE: To explore the potential role of ultrasound (US) as a bedside tool in intensive care unit patients with presumed neurogenic heterotopic ossification (NHO). SETTING: Tertiary university teaching hospitals. PARTICIPANTS: Critical care patients with acquired brain injury. DESIGN: A multicenter prospective study of critical care patients with possible NHO. MAIN MEASURES: Following a screening clinical examination, the major joints were evaluated with US, serum laboratory work, and x-ray...
February 10, 2017: Journal of Head Trauma Rehabilitation
Eitan M Kohan, Peter N Chalmers, Dane Salazar, Jay D Keener, Ken Yamaguchi, Aaron M Chamberlain
BACKGROUND: The etiology of instability following reverse total shoulder arthroplasty (RTSA) remains incompletely understood. The purpose of this study was to describe the shared characteristics, etiologies, and outcomes of early and late dislocations requiring operative revision. METHODS: We identified all patients at our institution who underwent operative revision of an RTSA for instability. Baseline demographic, clinical, and radiographic data were collected...
February 3, 2017: Journal of Shoulder and Elbow Surgery
Shallini Mittal, Samita Singal, Amit Mittal, Rikki Singal, Gunjan Jindal
The foramen of Huschke, or foramen tympanicum, is a developmental defect in the bony part of the external auditory canal (EAC) due to a defect in ossification of normal bone in the first 5 years of life. Seen in the anteroinferior part of the EAC, it can be asymptomatic or present with salivary discharge into the EAC during mastication or as soft tissue swelling in the EAC in the absence of any inflammation, tumor, or trauma. We present a case of a man who presented with herniation of soft tissues of the temporomandibular joint into the EAC through a persistent foramen tympanicum...
January 2017: Proceedings of the Baylor University Medical Center
Yu Chen, Haisong Yang, Jinhao Miao, Xiaowei Liu, Xinwei Wang, Deyu Chen
STUDY DESIGN: In vitro molecular research on the posterior longitudinal ligament fibroblasts. OBJECTIVE: To investigate different expression of old astrocyte specifically induced substance (OASIS) between spinal ligament fibroblasts from the patients with ossification of the posterior longitudinal ligament (OPLL) and from non-OPLL patients and demonstrate knockdown of OASIS protein expression by RNA interference inhibiting expression of type I collagen (COL I) in OPLL cells...
February 2017: Clinical Spine Surgery
Jia Shao, Miao Yu, Liang Jiang, Fengliang Wu, Xiaoguang Liu
The purpose of this study was to detect the differentially expressed genes between ossified herniated discs and herniated discs without ossification. In addition, we sought to identify a few candidate genes and pathways by using bioinformatics analysis. We analyzed 6 samples each of ossified herniated discs (experimental group) and herniated discs without ossification (control group). Purified mRNA and cDNA extracted from the samples were subjected to sequencing. The NOISeq method was used to statistically identify the differentially expressed genes (DEGs) between the 2 groups...
January 2017: International Journal of Molecular Medicine
Miao Yu, Si-Qi Yi, Yan-Ru Wu, Hua-Ling Sun, Fang-Fang Song, Jia-Wei Wang
Endochondral ossification is an essential skeletal development process which is strongly linked to chondrocyte differentiation. DNA damage-inducible transcript 3 (Ddit3), a member of the CCAAT/enhancer-binding protein family of transcription factors, is highly expressed in the cartilage plate. However, the role of DNA damage-inducible transcript 3 in chondrocyte differentiation remains to be investigated. Immunofluorescent staining was used to detect Ddit3 expression in the mouse growth plate and in the mouse chondroprogenitor cell line ATDC5...
December 2016: International Journal of Biochemistry & Cell Biology
Ying Shao, Qian-Zhao Chen, Yu-Hua Zeng, Yang Li, Wen-Yan Ren, Lin-Yun Zhou, Rong-Xin Liu, Ke Wu, Jun-Qing Yang, Zhong-Liang Deng, Yu Yu, Wen-Juan Sun, Bai-Cheng He
Rosiglitazone (RSG) is a potent drug used in the treatment of insulin resistance; however, it is associated with marked skeletal toxicity. RSG-induced osteoporosis may contribute to the promotion of adipogenic differentiation at the expense of osteogenic differentiation in bone marrow stromal cells. The aim of this study was to investigate whether RSG-induced bone toxicity can be reversed by combined treatment with all-trans retinoic acid (ATRA). We examined different osteogenic markers in mouse embryonic fibroblasts (MEFs) following treatment with RSG, ATRA, or RSG and ATRA in combination...
December 2016: International Journal of Molecular Medicine
Kai Hu, Tatiana Y Besschetnova, Bjorn R Olsen
BMP2 is widely used for promotion of bone repair and regeneration. However, bone formation induced by BMP2 is quite variable. Bone forming progenitor cells in different locations appear to respond to BMP2 in different ways, and repair outcomes can vary as a consequence of modulating effects by other factors. In this study, we have examined the effects of VEGF on BMP2-induced repair of a cortical bone defect, a 1 mm diameter drill hole, in the proximal tibia of mice. Treatment of the defect with either a bolus of PBS or soluble VEGFR1 (sVEGFR1), a decoy receptor for VEGF, had the same effects on bone formation via intramembranous ossification in the defect and cartilage formation and injured periosteum, during the healing process...
September 7, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
B Verhofste, T Decock, A Van Tongel, L De Wilde
AIMS: Heterotopic ossification (HO) occurs after arthroplasty, especially total hip arthroplasty. In this study we describe the incidence, evolution, morphology and clinical consequences of HO following reverse shoulder arthroplasty. PATIENTS AND METHODS: This is a single-centre retrospective study of 132 consecutive patients who received a Delta III or Delta Xtend reverse total shoulder arthroplasty between 2006 and 2013 for the treatment of cuff tear arthropathy...
September 2016: Bone & Joint Journal
Jordan J Allensworth, Karla D O'Dell, Joshua S Schindler
BACKGROUND: Diffuse idiopathic skeletal hyperostosis (DISH) is an idiopathic spinal disease common in the elderly and characterized by flowing ossification and osteophyte formation along the spinal column. Cervical hyperostosis is capable of producing dysphagia, stridor, and airway obstruction; however, there are no extant reports of true paralysis of bilateral vocal folds in patients fulfilling the criteria for DISH. METHODS AND RESULTS: We report a case of a 61-year-old man presenting with dysphagia and dyspnea...
January 2017: Head & Neck
Chong Chen, Caixia Xu, Taifeng Zhou, Bo Gao, Hang Zhou, Changhua Chen, Changli Zhang, Dongsheng Huang, Peiqiang Su
Abnormalities of membranous and endochondral ossification in patients with adolescent idiopathic scoliosis (AIS) remain incompletely understood. To investigate abnormalities in the melatonin signaling pathway and cellular response to melatonin in AIS, a case‑control study of osteogenic and chondrogenic differentiation was performed using human mesenchymal stem cells (hMSCs). AIS was diagnosed by physical and radiographic examination. hMSCs were isolated from the bone marrow of patients with AIS and control subjects (n=12 each), and purified by density gradient centrifugation...
August 2016: Molecular Medicine Reports
Jia-Wei Kevin Ko, Jeffrey D Tompson, Daniel S Sholder, Eric M Black, Joseph A Abboud
BACKGROUND: Heterotopic ossification (HO) around shoulder arthroplasty is a frequent finding with unclear clinical relevance. This study evaluated the incidence, relevance, and predisposing factors of HO in the long head of the triceps tendon after reverse shoulder arthroplasty. METHODS: Retrospective chart review was conducted to identify patients who had a reverse shoulder arthroplasty performed between 2008 and 2012. Patient demographics, implant types, and diagnoses were noted...
November 2016: Journal of Shoulder and Elbow Surgery
Yu Chen, Haisong Yang, Jinhao Miao, Xiaowei Liu, Xinwei Wang, Deyu Chen
STUDY DESIGN: Vitro molecular research on the posterior longitudinal ligament fibroblasts. OBJECTIVE: To investigate different expression of OASIS between spinal ligament fibroblasts from the patients with ossification of the posterior longitudinal ligament (OPLL) and non-OPLL patients and demonstrate knockdown of OASIS protein expression by RNA interference inhibiting expression of type I collagen in OPLL cells. SUMMARY OF BACKGROUND DATA: OPLL is characterized by ectopic bone formation in spinal ligaments...
May 11, 2016: Clinical Spine Surgery
Vaida Glatt, Nicole Bartnikowski, Nicholas Quirk, Michael Schuetz, Christopher Evans
BACKGROUND: Reverse dynamization is a technology for enhancing the healing of osseous defects. With use of an external fixator, the axial stiffness across the defect is initially set low and subsequently increased. The purpose of the study described in this paper was to explore the efficacy of reverse dynamization under different conditions. METHODS: Rat femoral defects were stabilized with external fixators that allowed the stiffness to be modulated on living animals...
April 20, 2016: Journal of Bone and Joint Surgery. American Volume
L Obert, R Saadnia, C Tournier, N Bonnevialle, D Saragaglia, F Sirveaux
INTRODUCTION: The reversed shoulder prosthesis is becoming the gold standard for cases of complex fracture of the proximal humerus after 70 years of age. MATERIAL AND METHODS: The French Society of Orthopaedic and Traumatology Surgery (SOFCOT) conducted a prospective and retrospective multicenter study to evaluate the results of the reversed shoulder prosthesis implanted in patients with a four-part fracture in nine centers. In the retrospective study (n=41 patients, 78 years of age, 14% ASA grade 3, 21% associated fractures) and in the prospective study (n=32 patients, 79 years of age, 37% ASA grade 3, 21% associated fractures) evaluation by independent surgeons was conducted to measure the QuickDASH score, the Constant score, the SSV (subjective shoulder value), and complications to correlate these measurements with radiological results...
May 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
Jian-Min Liu, Ya-Zhou Cui, Geng-Lin Zhang, Xiao-Yan Zhou, Jing-Xiang Pang, Xue-Zheng Wang, Jin-Xiang Han
BACKGROUND: Ankylosing spondylitis (AS) is the most common rheumatic condition that is slowly progressive and predominantly affects adolescents. Pathological bone formation associated with AS is an important cause of disability. The aim of the study was to investigate the possible involvement of the genes related to endochondral ossification and ectopia ossification in genetic susceptibility to AS in a Chinese Han population. METHODS: Sixty-eight single nucleotide polymorphisms (SNPs) from 13 genes were genotyped in discovery cohorts including 300 AS patients and 180 healthy controls...
March 20, 2016: Chinese Medical Journal
Joseph M Statz, Eric R Wagner, Matthew T Houdek, Robert H Cofield, Joaquin Sanchez-Sotelo, Bassem T Elhassan, John W Sperling
BACKGROUND: Obesity is a risk factor for worse outcomes in anatomic shoulder arthroplasty. The purpose of this investigation was to determine outcomes of primary reverse shoulder arthroplasty (RSA) in patients with morbid obesity. METHODS: We reviewed all primary RSAs performed on morbidly obese (body mass index [BMI] ≥40 kg/m(2)) patients from 2005 to 2012 at our institution with at least 2 years of follow-up. The study included 41 patients (57% female), the mean BMI was 44 kg/m(2), and the mean age was 67...
July 2016: Journal of Shoulder and Elbow Surgery
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