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https://www.readbyqxmd.com/read/29031210/effect-of-photoactivated-riboflavin-on-the-biodegradation-resistance-of-root-dentin-collagen
#1
Balasankar Meera Priyadarshini, Thong Beng Lu, Amr S Fawzy
This study was conducted to evaluate the effect of UVA-activated 1% riboflavin solution on structural integrity; mechanical properties and stability; and collagenase-mediated collagen solubilisation resistance of demineralized root dentin collagen matrix. Root dentin specimens demineralized with 17% EDTA for 7days were treated with 1% RF for 1min followed by UVA photo-activation at intensity 7mW/cm(2) for 1min. Control specimens were completely devoid of riboflavin and/or UVA treatments. Specimens were challenged with bacterial collagenase type-I solution for different time-periods at 37°C...
October 8, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29028629/a-dual-energy-micro-ct-methodology-for-visualization-and-quantification-of-biofilm-formation-and-dentin-demineralization
#2
Paula Maciel Pires, Thais Pires Dos Santos, Andrea Fonseca-Gonçalves, Matheus Melo Pithon, Ricardo Tadeu Lopes, Aline de Almeida Neves
OBJECTIVE: The aim of this study was to induce artificial caries in human sound dentin by means of a microcosm model using human saliva as source of bacteria and to apply a novel dual-energy micro-CT technique to quantify biofilm formation and evaluate its demineralization potential. DESIGN: Eight sound third molars had the occlusal enamel removed by cutting with a diamond disk and five cylindrical cavities (±2mm diameter; ±1.5mm depth) were prepared over the dentin surface in each specimen (n=40 cavities)...
October 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29024928/zinc-inhibits-collagenolysis-by-cathepsin-k-and-matrix-metalloproteinases-in-demineralized-dentin-matrix
#3
Pinar Altinci, Roda Seseogullari-Dirihan, Gulsen Can, David Pashley, Arzu Tezvergil-Mutluay
The enzymatic degradation of dentin organic matrix occurs via both the action of matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). Zinc can prevent collagen hydrolysis by MMPs. However, its effect on the activity of dentin-bound CCs is not known. The aim of this study was to investigate the effect of zinc on matrix-bound cathepsin K and MMP activity in dentin. Completely demineralized dentin beams were divided into test groups (n = 9) and incubated at 37°C in an incubation media (1 mL) containing ZnCl2 of 0...
October 13, 2017: Caries Research
https://www.readbyqxmd.com/read/29018937/local-effect-of-zoledronic-acid-on-new-bone-formation-in-posterolateral-spinal-fusion-with-demineralized-bone-matrix-in-a-murine-model
#4
Pawel Zwolak, Jan Farei-Campagna, Thorsten Jentzsch, Brigitte von Rechenberg, Clément M Werner
BACKGROUND: Posterolateral spinal fusion is a common orthopaedic surgery performed to treat degenerative and traumatic deformities of the spinal column. In posteriolateral spinal fusion, different osteoinductive demineralized bone matrix products have been previously investigated. We evaluated the effect of locally applied zoledronic acid in combination with commercially available demineralized bone matrix putty on new bone formation in posterolateral spinal fusion in a murine in vivo model...
October 10, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28992519/posterior-atlantoaxial-fusion-with-a-screw-rod-system-allograft-versus-iliac-crest-autograft
#5
Da-Geng Huang, Xin-Liang Zhang, Ding-Jun Hao, Cheng-Cheng Yu, Bai-Bing Mi, Qi-Ling Yuan, Bao-Rong He, Tuan-Jiang Liu, Hua Guo, Xiao-Dong Wang
OBJECTIVE: To compare the effectiveness of allograft and iliac crest autograft in atlantoaxial fusion. PATIENTS AND METHODS: Between January 2012 and December 2012, 41 consecutive patients underwent posterior atlantoaxial fusion with a screw-rod fixation system in our spine center. The choice to use allograft or iliac crest autograft was made by the patient himself or herself after being informed about the advantages and disadvantages of both methods. In the allograft group, we used mixed material of morcellized demineralized freeze-dried bone allograft and local autograft for posterior atlantoaxial fusion...
October 3, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28989121/protective-effect-of-adhesive-systems-associated-with-neodymium-doped-yttrium-aluminum-garnet-laser-on-enamel-erosive-abrasive-wear
#6
Erica Crastechini, Alessandra B Borges, Klaus Becker, Thomas Attin, Carlos Rg Torres
AIM: This study evaluated the efficacy of self-etching adhesive systems associated or not associated with the neodymium-doped yttrium aluminum garnet (Nd:YAG) laser on the protection against enamel erosive/abrasive wear. MATERIALS AND METHODS: Bovine enamel specimens were demineralized with 0.3% citric acid (5 minutes). The samples were randomly assigned to eight groups (n = 20): SB - Single Bond Universal (3M/ESPE); SB+L - Single Bond Universal + laser (80 mJ/10 Hz); FB - Futurabond U (Voco); FB+L -Futurabond U + laser; GEN - G-aenial bond (GC); GEN+L -G-aenial bond + laser; L - laser irradiation; and C - no treatment...
October 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28987724/effect-of-proanthocyanidin-enriched-extracts-on-the-inhibition-of-wear-and-degradation-of-dentin-demineralized-organic-matrix
#7
Ana Paula Boteon, Melissa Thiemi Kato, Marília Afonso Rabelo Buzalaf, Anuradha Prakki, Linda Wang, Daniela Rios, Heitor Marques Honório
OBJECTIVES: The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gels in inhibiting wear and degradation of demineralized organic matrix (DOM). DESIGN: 225 dentin specimens obtained from bovine incisors were randomly allocated into 5 groups (n=45): 10% Grape seed extract gel (GSE), 10% Cranberry extract gel (CE), 0.012% Chlorhexidine gel (CX), 1.23% NaF gel (F), and no active compound gel (P, placebo). Before the treatments, samples were demineralized by immersion in 0...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28987248/the-effect-of-mipaste-plus-and-reminpro-on-incipient-caries-using-diagnodent-and-sem-an-invitro-study
#8
Bassir Leila, Saeid Nemati, Hajalizadeh Neda, Mashallah Khanehmasjedi
BACKGROUND: The remineralization of early caries lesion has an effective role on decreasing caries. For initial remineralization of caries lesion, using Compounds of Casein phosphopeptides (CPP) in different studies has been proposed. REMINPRO including Fluoride, Xylitol and Calcium phosphate has just been offered in the market. This study aims to investigate the efficacy of Reminpro and MI paste plus in terms of remineralization of early enamel lesions. MATERIALS AND METHODS: In 15 samples of healthy enamels of the maxillary first premolar teeth and 15 samples of Primary Mandibular First Molar under the effect of demineralized gel, artificial caries has been created...
2017: Journal of the National Medical Association
https://www.readbyqxmd.com/read/28986227/anterior-cervical-discectomy-with-instrumented-allograft-fusion-lordosis-restoration-and-comparison-of-functional-outcomes-among-patients-of-different-age-groups
#9
Dario Muzevic, Bruno Splavski, Frederick A Boop, Kenan I Arnautovic
OBJECTIVE: To investigate clinical parameters of anterior cervical discectomy and fusion (ACDF) treatment and outcomes using osseous allograft in different age groups, study postoperative results of restoring of lordosis, and evaluate the utility of bone allografts for ACDF, including graft subsidence. METHODS: We reviewed data from 154 patients having clinical symptoms and radiological signs of disc herniation and/or cervical spondylosis. Decompression was achieved through discectomy, osteophyte ablation, endplate drilling, and foraminotomy...
October 3, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28984044/an-engineered-tendon-ligament-bioscaffold-derived-from-decellularized-and-demineralized-cortical-bone-matrix
#10
Jie-Liang Yang, Xuan Yao, Quan Qing, Yi Zhang, Yan-Lin Jiang, Liang-Ju Ning, Jing-Cong Luo, Ting-Wu Qin
Demineralized bone matrix (DBM), as an extracellular matrix (ECM), has had limited use as a medical replacement although studies have reported a possibility for its use in tendon or ligament tissue engineering. To be an acid-extracted organic matrix, DBM contains much of bone protein, with a small amount of inorganic solids and some cell debris. However, cell debris is a critical factor that triggers inflammatory reaction in clinical reconstructions using ECM. In this study, we used a protocol incorporating the use of detergent with nuclease treatment to prepare decellularized DBM (DCDBM)...
October 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28983253/a-novel-kinetic-method-to-measure-apparent-solubility-product-of-bulk-human-enamel
#11
Linda Hassanali, Ferranti S Wong, Richard J M Lynch, Paul Anderson
Introduction: Tooth enamel mineral loss is influenced by its solubility product value, which is fundamental to the understanding of de- and remineralization resulting from a carious or erosive challenge. Published pKsp values for human enamel and hydroxyapatite range from 110 to 126 suggesting a heterogeneous nature of enamel solubility. However, this range of values may also result from the variety of methods used, e.g., some authors reporting values for suspensions of enamel powder and others for bulk enamel...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28979574/comparison-of-composition-and-anticaries-effect-of-galla-chinensis-extracts-with-different-isolation-methods
#12
Xuelian Huang, Meng Deng, Mingdong Liu, Lei Cheng, R A M Exterkate, Jiyao Li, Xuedong Zhou, Jacob M Ten Cate
OBJECTIVES: Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention. METHODS: The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared...
2017: Open Dentistry Journal
https://www.readbyqxmd.com/read/28977796/efficacy-of-stannous-ions-on-enamel-demineralization-under-normal-and-hyposalivatory-conditions-a-controlled-randomized-in-situ-pilot-trial
#13
Núbia Inocencya Pavesi Pini, Nadine Schlueter, Daniel Sundfeld, Wiebke Semper Hogg, Alan Roger Santos-Silva, Marcio Ajudarte Lopes, Flávio H B Aguiar, J R Lovadino, D A N L Lima
The study aim was to investigate the effect of antierosive agents on enamel under normal and hyposalivatory conditions. This double-blind crossover in situ pilot study evaluated 4 toothpastes: placebo (0 ppm F), sodium fluoride (NaF, 1,450 ppm), stannous/sodium fluoride (SnF/NaF, 1,450 ppm F-, 1,090 ppm Sn2+), and sodium fluoride, stannous chloride and chitosan (NaF/Sn/Ch, 1,450 ppm F-, 3,500 ppm Sn2+, 0.5% Ch). Twenty participants were assigned to 2 groups (n = 10 each): normal and low salivary flow. Participants wore palatal appliances holding 4 bovine enamel specimens previously eroded in vitro (D1) for 20 min prior to an in situ phase after which they were eroded again (D2)...
January 5, 2017: Caries Research
https://www.readbyqxmd.com/read/28969280/root-conditioning-with-citric-acid-and-ethylenediaminetetraacetic-acid-and-their-effect-on-fibrin-clot-adhesion-to-dentin-a-scanning-electron-microscopic-study
#14
Sangeetha Subramanian, Devapriya Appukuttan, Anupama Tadepalli, Prakash Ponnudurai Samuel Gnana, Rajagopal Thennukonda Athmarao
INTRODUCTION: Root biomodifiers are used to remove the smear layer formed on the roots as a result of mechanical debridement and also to expose collagen fibers making the root surfaces biologically acceptable. Further, the blood element linkage to demineralized roots and clot stabilization by collagen fibres are vital for the wound healing and success of periodontal surgery. AIM: The aim of this study was to evaluate and compare the distribution of fibrin network to dentin surfaces conditioned with citric acid and Ethylenediaminetetraacetic Acid (EDTA) using Scanning Electron Microscope (SEM)...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28966064/in%C3%A2-vitro-assessment-of-stainless-steel-orthodontic-brackets-coated-with-titanium-oxide-mixed-ag-for-anti-adherent-and-antibacterial-properties-against-streptococcus-mutans-and-porphyromonas-gingivalis
#15
Eman Jameel Fatani, Hamed H Almutairi, Ali O Alharbi, Yasser Obaidallah Alnakhli, Darshan Devang Divakar, Muzaheed, Abdulaziz Abdullah Alkheraif, Aftab Ahmed Khan
Orthodontic brackets made from stainless steel were introduced in dentistry, though they have less ability in reducing enamel demineralization and are not successful in preventing microbial as well as biofilm growth. In this study, we evaluated the significant role of different brackets in reducing enamel demineralization indirectly. Results from different tests indicate the significant reduction in adhesion, biofilm formation and slow growth of tested bacterial species on brackets coated with Ag + TiO2 and found to be statistically significant lower than control...
September 29, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28959764/antimicrobial-effect-of-copper-oxide-nanoparticles-on-some-oral-bacteria-and-candida-species
#16
M Amiri, Z Etemadifar, A Daneshkazemi, M Nateghi
STATEMENT OF PROBLEM: Acid producing bacteria including Streptococcus mutans and lactobacilli cause tooth demineralization and lead to tooth decay. Also, oral colonization of the species of Candida has been reported in many studies that are resistant to antifungal agents. OBJECTIVES: In this study, antibacterial and antifungal effects of nano-CuO were studied against some oral bacteria and yeast fungi. MATERIALS AND METHODS: The minimum inhibitory concentrations (MICs) of copper oxide nanoparticles (CuO NPs) for oral bacterial and fungal test strains were determined in 96-well microtiter plate technique...
March 2017: J Dent Biomater
https://www.readbyqxmd.com/read/28955696/atraumatic-bilateral-fracture-of-the-femoral-neck-in-young-male-patient-with-suspected-osteomalacia
#17
Byung-Ho Yoon, Min-Soo Kwon
In this report, we describe the case of a healthy 37-year-old male patient without a history of disease, who developed atraumatic bilateral fracture of the femoral neck. Radiological and blood investigations revealed osteopenia and severe vitamin D deficiency (7.42 ng/mL), respectively, but patient had no apparent risk factors for insufficiency fracture. Bilateral osteosynthesis was obtained using cannulated screws and laboratory findings improved after vitamin D supplementation. This case highlights the effect of vitamin D deficiency on demineralization in a young male patient...
August 2017: Journal of Bone Metabolism
https://www.readbyqxmd.com/read/28955221/hypoglycemic-potential-of-aqueous-extract-of-moringa-oleifera-leaf-and-in-vivo-gc-ms-metabolomics
#18
Washim Khan, Rabea Parveen, Karishma Chester, Shabana Parveen, Sayeed Ahmad
Moringa oleifera Lam. (family; Moringaceae), commonly known as drumstick, have been used for centuries as a part of the Ayurvedic system for several diseases without having any scientific data. Demineralized water was used to prepare aqueous extract by maceration for 24 h and complete metabolic profiling was performed using GC-MS and HPLC. Hypoglycemic properties of extract have been tested on carbohydrate digesting enzyme activity, yeast cell uptake, muscle glucose uptake, and intestinal glucose absorption...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28954939/comparison-of-resin-based-and-glass-ionomer-sealants-with-regard-to-fluoriderelease-and-anti-demineralization-efficacy-on-adjacent-unsealed-enamel
#19
Thwe Zin Ei, Yasushi Shimada, Syozi Nakashima, Maria Jacinta Rosario H Romero, Yasunori Sumi, Junji Tagami
This study compared resin-based and glass ionomer sealants with regard to their fluoride-release behavior and anti-demineralization potential on adjacent unsealed enamel surfaces. Sealant cavities prepared on bovine enamel blocks were filled with fluoride-containing resin sealants [TeethmateF-1 (TF), Clinpro(TM) (CP)], and glass ionomer sealant [Fuji VII (FVII)]. Specimens were then incubated in artificial saliva for 14 days to measure fluoride. Thereafter, demineralization was performed for 10 days, and the anti-demineralization efficacy was assessed by Swept Source Optical Coherence Tomography (SS-OCT), and cross-sectional nanohardness...
September 28, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28953929/class-ii-and-iv-hdacs-function-as-inhibitors-of-osteoclast-differentiation
#20
Nicholas C Blixt, Bora K Faulkner, Kristina Astleford, Rosemary Lelich, Jacob Schering, Ekaterina Spencer, Rajaram Gopalakrishnan, Eric D Jensen, Kim C Mansky
Histone deacetylases (HDACs) are negative regulators of transcription and have been shown to regulate specific changes in gene expression. In vertebrates, eighteen HDACs have thus far been identified and subdivided into four classes (I-IV). Key roles for several HDACs in bone development and biology have been elucidated through in vitro and in vivo models. By comparison, there is a paucity of data on the roles of individual HDACs in osteoclast formation and function. In this study, we investigated the gene expression patterns and the effects of suppressing individual class II (Hdac4, 5, 6, 9, and 10) and class IV (Hdac11) HDACs during osteoclast differentiation...
2017: PloS One
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