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https://www.readbyqxmd.com/read/29352218/nutritional-deficiencies-and-bone-metabolism-after-endobarrier-in-obese-type-2-patients-with-diabetes
#1
Nuria Vilarrasa, Alexandra Fabregat, Silvia Toro, Amador García Gordejuela, Anna Casajoana, Mónica Montserrat, Pilar Garrido, Rafael López-Urdiales, Núria Virgili, Alejandra Planas-Vilaseca, Andreu Simó-Servat, Jordi Pujol
Endobarrier® is a minimally invasive, reversible endoscopic treatment for obesity. It provokes malabsorption along 60 cm of the small intestine, which can contribute to the development of vitamin deficiencies and to changes in bone mineral density (BMD). To determine the prevalence of nutrient deficiencies, changes in body composition and BMD during the first year after Endobarrier® placement. Twenty-one patients with type 2 diabetes met inclusion criteria. Levels of vitamins, micro and macronutrients were assessed prior and at 1, 3 and 12 months post-operatively...
January 19, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29344873/laser-capture-microdissection-and-rna-extraction-from-perfusion-fixed-cartilage-and-bone-tissue-from-mice-implanted-with-human-ipsc-derived-mscs-in-a-calvarial-defect-model
#2
Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, Joel S Pachter
Laser-capture microdissection (LCM) coupled to downstream RNA analysis poses unique difficulties for the evaluation of mineralized tissues. A rapid protocol was thus developed to enable sufficient integrity of bone and cartilage tissue for reliable sectioning, while minimizing RNA loss associated with prolonged decalcification and purification steps. Specifically, the protocol involves pump-assisted, cardiac perfusion-fixation with paraformaldehyde, and moderate digestion of LCM-acquired tissue with proteinase K followed by DNase treatment and separation of RNA using magnetic beads...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29342370/fgf8-signaling-alters-the-osteogenic-cell-fate-in-the-hard-palate
#3
J Xu, Z Huang, W Wang, X Tan, H Li, Y Zhang, W Tian, T Hu, Y P Chen
Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulate the osteogenic cell fate in the developing palate remain unclear. In the present study, we investigated the potential role of FGF signaling in osteogenic fate determination of the palatal mesenchymal cells...
January 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29332280/hydrogeochemical-processes-and-influence-of-seawater-intrusion-in-coastal-aquifers-south-of-chennai-tamil-nadu-india
#4
G Kanagaraj, L Elango, S G D Sridhar, G Gowrisankar
Seawater intrusion promotes the salinity of groundwater, and it poses a great environmental impact on a global scale. The present study was carried out to determine the hydrogeochemical processes and influence of seawater intrusion in the coastal aquifers using geophysical, geochemical, and stable isotope techniques. The true resistivity value ranges from 0.5 to 8008.5 Ω-m which has been measured using vertical electrical sounding (VES) based on the Schlumberger method. About 33 groundwater samples were collected during post-monsoon (POM) (January 2012) and pre-monsoon (PRM) (June 2012) seasons from open and bore wells and were analyzed for major ions and stable isotopes...
January 14, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29327231/loading-of-the-condylar-cartilage-can-rescue-the-effects-of-botox-on-tmj
#5
Eliane H Dutra, Mara H O'Brien, Candice Logan, Aditya Tadinada, Ravindra Nanda, Sumit Yadav
The purpose of this study is to evaluate whether the effects of botulinum neurotoxin (botox) injection into the masseter in the mandibular condylar cartilage (MCC) and subchondral bone could be rescued by compressive loading of the temporomandibular joint (TMJ). Twenty-four 6-week-old female mice (C57BL/6J) were used. Mice were divided in three groups: (1) Botox (n = 8); (2) Botox plus loading (n = 8); (3) Pure control (n = 8). Bone labels (3 and 1 day before sacrifice) and the proliferation marker EdU (2 and 1 day before sacrifice) were intraperitoneally injected into all groups of mice...
January 11, 2018: Calcified Tissue International
https://www.readbyqxmd.com/read/29320475/warfare-and-wildlife-declines-in-africa-s-protected-areas
#6
Joshua H Daskin, Robert M Pringle
Large-mammal populations are ecological linchpins, and their worldwide decline and extinction disrupts many ecosystem functions and services. Reversal of this trend will require an understanding of the determinants of population decline, to enable more accurate predictions of when and where collapses will occur and to guide the development of effective conservation and restoration policies. Many correlates of large-mammal declines are known, including low reproductive rates, overhunting, and habitat destruction...
January 10, 2018: Nature
https://www.readbyqxmd.com/read/29314550/the-phosphorus-rich-signature-of-fire-in-the-soil-plant-system-a-global-meta-analysis
#7
Orpheus M Butler, James J Elser, Tom Lewis, Brendan Mackey, Chengrong Chen
The biogeochemical and stoichiometric signature of vegetation fire may influence post-fire ecosystem characteristics and the evolution of plant 'fire traits'. Phosphorus (P), a potentially limiting nutrient in many fire-prone environments, might be particularly important in this context; however, the effects of fire on P cycling often vary widely. We conducted a global-scale meta-analysis using data from 174 soil studies and 39 litter studies, and found that fire led to significantly higher concentrations of soil mineral P as well as significantly lower soil and litter carbon:P and nitrogen:P ratios...
January 4, 2018: Ecology Letters
https://www.readbyqxmd.com/read/29306537/metformin-enhances-the-differentiation-of-dental-pulp-cells-into-odontoblasts-by-activating-ampk-signaling
#8
Wei Qin, Xianling Gao, Tao Ma, Michael D Weir, Jing Zou, Bing Song, Zhengmei Lin, Abraham Schneider, Hockin H K Xu
INTRODUCTION: Metformin is a first-line drug for treating type 2 diabetes that regulates the differentiation of mesenchymal stem cells. Its effects on human dental pulp cells (DPCs) remain unknown. This study aimed to investigate the effects of metformin on the proliferation and differentiation of DPCs. METHODS: A live/dead viability assay kit was used to examine the effects of metformin on the cell viability of DPCs. Cell proliferation was analyzed using a cell counting kit (CCK-8; Dojindo, Tokyo, Japan)...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29305863/mir-449-overexpression-inhibits-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-via-suppressing-sirt1-fra-1-pathway-in-high-glucose-and-free-fatty-acids-microenvironment
#9
Bo Qu, Kai Gong, Hong-Sheng Yang, Yu-Gang Li, Tao Jiang, Zhi-Mou Zeng, Zong-Rui Cao, Xian-Ming Pan
Diabetic osteoporosis is a chronic complication caused by diabetes mellitus, and However, the exact mechanism of diabetes mellitus-induced osteoporosis is still unknown. In this study, we investigate the effect of miR-449 on osteogenic differentiation and its underlying mechanism in human bone marrow-derived mesenchymal stem cells (hBMSCs) with high glucose (HG) and free fatty acids (FFA) treatment. Results showed that after culturing for 14 days, high glucose (HG) and free fatty acids (FFA) treatment dramatically decreased mineralization of human bone marrow-derived mesenchymal stem cells (hBMSCs) compared with cells treated with osteogenic medium (OM) alone...
January 3, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29286152/a-promising-novel-formulation-for-articular-cartilage-regeneration-preclinical-evaluation-of-a-treatment-that-produces-sox9-overexpression-in-human-synovial-fluid-cells
#10
Ivan Delgado-Enciso, Juan Paz-Garcia, Alejandrina Rodriguez-Hernandez, Violeta M Madrigal-Perez, Ariana Cabrera-Licona, Alejandro Garcia-Rivera, Alejandro D Soriano-Hernandez, Jose L Cortes-Bazan, Hector R Galvan-Salazar, Jose Valtierra-Alvarez, Jose Guzman-Esquivel, Iram P Rodriguez-Sanchez, Margarita L Martinez-Fierro, Brenda Paz-Michel
Osteoarthritis (OA) is a chronic disorder of synovial joints, in which there is progressive softening and disintegration of the articular cartilage. OA is the most common form of arthritis, and is the primary cause of disability and impaired quality of life in the elderly. Despite considerable medical necessity, no treatment has yet been proven to act as a disease‑modifying agent that may halt or reverse the structural progression of OA. The replacement of the joint with a prosthesis appears to be the best option in the advanced stages of the disease...
December 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29285077/identification-of-micrornas-in-human-circulating-monocytes-of-postmenopausal-osteoporotic-mexican-mestizo-women-a-pilot-study
#11
Rogelio F Jiménez-Ortega, Eric G Ramírez-Salazar, Alma Y Parra-Torres, Said A Muñoz-Montero, Claudia Rangel-Escareňo, Ivan Salido-Guadarrama, Mauricio Rodriguez-Dorantes, Manuel Quiterio, Jorge Salmerón, Rafael Velázquez-Cruz
MicroRNAs (miRNAs or miRs) are a class of short non-coding RNAs that serve an important regulatory role in living organisms. These molecules are associated with multiple biological processes and are potential biomarkers in multiple diseases. The present study aimed to further identify miRNAs that are differentially expressed in circulating monocytes (CMCs) from postmenopausal Mexican-Mestizo women. Microarray analyses of monocytes using Affymetrix miRNA 4.0 and Human Genome U133 Plus 2.0 arrays were performed in 6 normal and 6 osteoporotic women, followed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29279016/mental-health-in-underground-coal-miners
#12
Alice Constantino Joaquim, Maurício Lopes, Luana Stangherlin, Kamila Castro, Luciane Bisognin Ceretta, Willians Cassiano Longen, Fabiane Ferraz, Ingrid D Schweigert Perry
As mental health goes beyond the simple absence of mental disorders, this paper characterized mental health components in 89 underground coal miners in southern Brazil. This is a cross-sectional study, that detected a low prevalence of depression; light or moderate anxiety in 13% of the workers; self-perception of good health; life quality and good psychological capacity in most of the participants; poor sleep quality in half of them; reverse correlations between anxiety and life quality, and anxiety and psychological capital; positive correlations between psychological capital and life quality; associations between self-perception of health, time, and sleep quality; associations between anxiety and sleep duration and quality; and between alcohol consumption and location at work (front, rear, or variable)...
December 26, 2017: Archives of Environmental & Occupational Health
https://www.readbyqxmd.com/read/29275850/outcomes-of-direct-pulp%C3%A2-capping-by-using-either-proroot-mineral-trioxide-aggregate-or-biodentine-in-permanent-teeth-with-carious-pulp-exposure-in-6-to-18-year-old-patients-a-randomized-controlled-trial
#13
Nuttaporn Parinyaprom, Areerat Nirunsittirat, Patchanee Chuveera, Sakarat Na Lampang, Tanida Srisuwan, Thanapat Sastraruji, Puangporn Bua-On, Sophon Simprasert, Issaraporn Khoipanich, Thitida Sutharaphan, Suthida Theppimarn, Narumol Ue-Srichai, Waritorn Tangtrakooljaroen, Papimon Chompu-Inwai
INTRODUCTION: This study aimed to compare the success rates of direct pulp capping (DPC) by using either ProRoot Mineral Trioxide Aggregate (MTA) or Biodentine in the cariously exposed permanent teeth of 6- to 18-year-old patients. Gray discoloration was also evaluated. METHODS: Fifty-nine cariously exposed permanent teeth, including teeth with diagnosis of normal pulp, reversible pulpitis, or irreversible pulpitis, early periapical involvement, and exposure size of up to 2...
December 21, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29273059/biomechanical-properties-of-3d-printed-bone-scaffolds-are-improved-by-treatment-with-crfp
#14
Carlos G Helguero, Vamiq M Mustahsan, Sunjit Parmar, Sahana Pentyala, John P Pfail, Imin Kao, David E Komatsu, Srinivas Pentyala
BACKGROUND: One of the major challenges in orthopedics is to develop implants that overcome current postoperative problems such as osteointegration, proper load bearing, and stress shielding. Current implant techniques such as allografts or endoprostheses never reach full bone integration, and the risk of fracture due to stress shielding is a major concern. To overcome this, a novel technique of reverse engineering to create artificial scaffolds was designed and tested. The purpose of the study is to create a new generation of implants that are both biocompatible and biomimetic...
December 22, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29260905/lncrna-tsix-promotes-osteoblast-apoptosis-in-particle-induced-osteolysis-by-down-regulating-mir-30a-5p
#15
Yanmin Bu, Dezhi Zheng, Lei Wang, Jun Liu
OBJECTIVE: This study aims to investigate the role of TSIX/miR-30a-5p axis in particle-induced osteolysis (PIO). METHOD: PIO mouse model was established by the implantation of Co-Cr-Mo metal particles (CoPs). MC3T3-E1 cells received CoPs stimulation. Bone mineral density (BMD) in the skull was detected to evaluate PIO development. The expression of TSIX and miR-30a-5p was detected by using qRT-PCR. Osteoblast apoptosis was measured using flow cytometry. RNA pull-down was used to verify the regulatory relationship between TSIX and miR-30a-5p...
December 20, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29251314/periodontal-ligament%C3%A2-associated-protein%C3%A2-1-delays-rat-periodontal-bone-defect-repair-by-regulating-osteogenic-differentiation-of-bone-marrow-stromal-cells-and-osteoclast-activation
#16
Xijiao Yu, Shuang Liu, Wei Wang, Shu Li
The aim of the present study was to assess the roles of periodontal ligament‑associated protein‑1 (PLAP‑1) in the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs) and in osteoclast activation during the repair of rat periodontal bone defects. Male, 6‑week‑old, Wistar rats treated with periodontal bone defects were randomly assigned to 3 groups: The PLAP‑1‑transfected rBMSC group (PLAP‑1 group), the empty vector‑transfected rBMSC group (vector group) and the normal rBMSC group (control group)...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29250156/restoration-of-osteogenic-differentiation-by-overexpression-of-cannabinoid-receptor-2-in-bone-marrow-mesenchymal-stem-cells-isolated-from-osteoporotic-patients
#17
Bangjun Wang, Kai Lian, Jun Li, Gang Mei
Cannabinoid receptor 2 (CNR2) has a critical role in osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). CNR2 expression was found to be downregulated in osteoporotic patients. The present study aimed to investigate the functionality of CNR2 in restoring osteogenic differentiation and mineralization of BMSCs isolated from osteoporotic patients. CNR2 was overexpressed in osteoporotic BMSCs by a lentivirus. Alkaline phosphatase (ALP) activity staining and alizarin red S staining were performed to examine the osteogenic differentiation of osteoporotic BMSCs...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29238917/cadmium-testicular-toxicity-in-male-wistar-rats-protective-roles-of-zinc-and-magnesium
#18
Nasim Babaknejad, Somaye Bahrami, Ali Asghar Moshtaghie, Hashem Nayeri, Parvin Rajabi, Farhad Golshan Iranpour
Cadmium (Cd) is a highly toxic element, which may cause toxicity to most organs in the body. Zinc (Zn) and magnesium (Mg) are essential minerals with probable benefits on Cd harmful effects. Finding an efficient and non-pathological treatment against Cd toxicity seems promising. Fifty adult rats were divided into ten experimental groups of five rats each. The Cd group was treated with 1 mg Cd/kg and the control group received 0.5 cm3 normal saline. The other eight groups received Zn (0.5 and 1.5 mg/kg) and Mg (0...
December 14, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/29232479/metabolomic-pathways-to-osteoporosis-in-middle-aged-women-a-genome-metabolome-wide-mendelian-randomization-study
#19
Alireza Moayyeri, Ching-Lung Cheung, Kathryn Cb Tan, John A Morris, Agustin Cerani, Robert P Mohney, J Brent Richard, Christopher Hammond, Tim D Spector, Cristina Menni
The metabolic state of the body can be a major determinant of bone health. We used a Mendelian Randomization approach to identify metabolites causally associated with bone mass to better understand the biological mechanisms of osteoporosis. We tested bone phenotypes (femoral neck, total hip, and lumbar spine BMD) for association with 280 fasting blood metabolites in 6055 women from TwinsUK cohort with genome-wide genotyping scans. Causal associations between metabolites and bone phenotypes were further assessed in a bidirectional Mendelian Randomization study using genetic markers/scores as instrumental variables...
December 12, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29230199/resource-legacies-of-organic-and-conventional-management-differentiate-soil-microbial-carbon-use
#20
Melissa M Arcand, David J Levy-Booth, Bobbi L Helgason
Long-term contrasts in agricultural management can shift soil resource availability with potential consequences to microbial carbon (C) use efficiency (CUE) and the fate of C in soils. Isothermal calorimetry was combined with 13C-labeled glucose stable isotope probing (SIP) of 16S rRNA genes to test the hypothesis that organically managed soils would support microbial communities with greater thermodynamic efficiency compared to conventional soils due to a legacy of lower resource availability and a resultant shift toward communities supportive of more oligotrophic taxa...
2017: Frontiers in Microbiology
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