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https://www.readbyqxmd.com/read/28644106/viability-and-osteogenic-differentiation-of-human-periodontal-ligament-progenitor-cells-are-maintained-after-incubation-with-porphyromonas-gingivalis-protein-extract
#1
Mayra Laino Albiero, Rafael Nóbrega Stipp, Miki Taketomi Saito, Márcio Zaffalon Casati, Enilson Antonio Sallum, Francisco Humberto Nociti, Karina Gonzales Silvério
BACKGROUND: Porphyromonas gingivalis (Pg) is a major periodontal pathogen that contains immunostimulatory components. Periodontal ligament mesenchymal stem cells (PDLSCs) are responsible for regeneration of the periodontium that is lost due to periodontitis. Pathologic factors within the microenvironment that impair resident PDLSCs are not well understood. The present study investigated in vitro the effects of Pg protein extract (PgPE) on the biological properties of CD105-enriched PDL progenitor cell populations (PDL-CD105(+) cells)...
June 23, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28643220/denosumab-an-emerging-therapy-in-pediatric-bone-disorders
#2
REVIEW
Alison M Boyce
PURPOSE OF REVIEW: Denosumab is an inhibitor of receptor activator of nuclear factor kappa-B ligand (RANKL), and has emerged as an important novel therapy for skeletal disorders. This article examines the use of denosumab in children. RECENT FINDINGS: Considerable safety and efficacy data exists for denosumab treatment of adults with osteoporosis, bone metastases, and giant cell tumors. Pediatric data is limited; however, evidence suggests denosumab may be beneficial in decreasing bone turnover, increasing bone density, and preventing growth of certain skeletal neoplasms in children...
June 22, 2017: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/28639995/recovery-of-bone-mineral-density-following-discontinuation-of-tenofovir-based-hiv-pre-exposure-prophylaxis
#3
David V Glidden, Kathleen Mulligan, Vanessa McMahan, Peter L Anderson, Juan Guanira, Suwat Chariyalertsak, Susan P Buchbinder, Linda Gail Bekker, Mauro Schechter, Beatriz Grinsztejn, Robert M Grant
BACKGROUND: Oral tenofovir disoproxil fumarate (TDF) for HIV prevention and treatment is associated with decreases in bone mineral density (BMD). Previous reports suggest that these changes may be reversible after discontinuation of TDF. SETTING: A metabolic substudy of 498 participants in a randomized, placebo-controlled HIV prevention trial of oral co-formulated TDF with emtricitabine (TDF/FTC, Truvada®) for HIV preexposure prophylaxis (PrEP) enrolling a global sample of men who have sex with men and trans women...
June 19, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28621459/microtubule-actin-crosslinking-factor-1-promotes-osteoblast-differentiation-by-promoting-%C3%AE-catenin-tcf1-runx2-signaling-axis
#4
Lifang Hu, Peihong Su, Chong Yin, Yan Zhang, Runzhi Li, Kun Yan, Zhihao Chen, Dijie Li, Ge Zhang, Liping Wang, Zhiping Miao, Airong Qian, Cory J Xian
Osteoblast differentiation is a multistep process delicately regulated by many factors, including cytoskeletal dynamics and signaling pathways. Microtubule actin crosslinking factor 1 (MACF1), a key cytoskeletal linker, has been shown to play key roles in signal transduction and in diverse cellular processes; however, its role in regulating osteoblast differentiation is still needed to be elucidated. To further uncover the functions and mechanisms of action of MACF1 in osteoblast differentiation, we examined effects of MACF1 knockdown (MACF1-KD) in MC3T3-E1 osteoblastic cells on their osteoblast differentiation and associated molecular mechanisms...
June 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28617442/titanium-particle-induced-osteogenic-inhibition-and-bone-destruction-are-mediated-by-the-gsk-3%C3%AE-%C3%AE-catenin-signal-pathway
#5
Ye Gu, Zhirong Wang, Jiawei Shi, Liangliang Wang, Zhenyang Hou, Xiaobin Guo, Yunxia Tao, Xiexing Wu, Wei Zhou, Yu Liu, Wen Zhang, Yaozeng Xu, Huilin Yang, Feng Xue, Dechun Geng
Wear debris-induced osteogenic inhibition and bone destruction are critical in the initiation of peri-prosthetic osteolysis. However, the molecular mechanism underlying this phenomenon is poorly understood. In this study, we analyzed the involvement of the GSK-3β/β-catenin signal pathway, which is important for bone formation in this pathological condition. We established a titanium (Ti) particle-stressed murine MC3T3-E1 cell culture system and calvariae osteolysis model to test the hypothesis that Ti particle-induced osteogenic inhibition and bone destruction are mediated by the GSK-3β/β-catenin signal pathway...
June 15, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28614950/grafting-of-architecture-controlled-poly-styrene-sodium-sulfonate-onto-titanium-surfaces-using-bio-adhesive-molecules-surface-characterization-and-biological-properties
#6
Hamza Chouirfa, Margaret D M Evans, David G Castner, Penny Bean, Dimitri Mercier, Anouk Galtayries, Céline Falentin-Daudré, Véronique Migonney
This contribution reports on grafting of bioactive polymers such as poly(sodium styrene sulfonate) (polyNaSS) onto titanium (Ti) surfaces. This grafting process uses a modified dopamine as an anchor molecule to link polyNaSS to the Ti surface. The grafting process combines reversible addition-fragmentation chain transfer polymerization, postpolymerization modification, and thiol-ene chemistry. The first step in the process is to synthetize architecture controlled polyNaSS with a thiol end group. The second step is the adhesion of the dopamine acrylamide (DA) anchor onto the Ti surfaces...
June 14, 2017: Biointerphases
https://www.readbyqxmd.com/read/28608628/the-role-of-microrna-143-5p-in-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts-by-targeting-runx2-via-the-opg-rankl-signaling-pathway
#7
Fu-Liang Zhan, Xin-Yang Liu, Xing-Bo Wang
This study aims to elucidate the mechanisms by which microRNA-143-5p (miR-143-5p) targets runt-related transcription factor 2 (Runx2) in the differentiation of dental pulp stem cells (DPSCs) into odontoblasts, through regulating the osteoprotegerin receptor activator of the nuclear factor-κB ligand (OPG/RANKL) signaling pathway. Following transfection, DPSCs were divided into blank, control, miR-143-5p mimics, miR-143-5p inhibitors, miR-143-5p inhibitors + siRunx2 and siRunx2 groups. Alkaline phosphatase (ALP) activity and mineralized nodules were detected using ALP kit and alizarin red staining...
June 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28606672/three-dimensional-sphere-forming-cells-are-unique-multipotent-cell-population-in-dental-pulp-cells
#8
Sung Hee Lee, Andrew Inaba, Neha Mohindroo, Deepika Ganesh, Charlotte Ellen Martin, Nadia Chugal, Reuben H Kim, Mo K Kang, No-Hee Park, Ki-Hyuk Shin
INTRODUCTION: Mesenchymal stem cells (MSCs) are typically cultured as adherent monolayer using a conventional tissue culture technique. However, this technique incompletely reproduces an in vivo microenvironment of stem cells and results in the loss of stemness properties. Three-dimensional (3D) sphere culture is one of the most widely used 3D culture techniques that have been developed to recapitulate the in vivo microenvironment. However, the stemness and multilineage differentiation capacity of spheres derived from dental pulp stem cells (DPSCs) have not been well investigated...
June 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28605233/clinoptilolite-for-treatment-of-dyslipidemia-preliminary-efficacy-study
#9
Milisav Cutovic, Milica Lazovic, Vesna Vukovic-Dejanovic, Dejan Nikolic, Ivana Petronic-Markovic, Dragana Cirovic
OBJECTIVES: A tribomechanically activated clinoptilolite (natural aluminosilicate mineral) has been used to increase growth in meat-producing animals, as an adjuvant in cancer therapy, and a heavy metal remover in humans. Because of its unique cation exchanging and chelating properties, we hypothesized that clinoptilolite may be beneficial for the treatment of dyslipidemia in the manner similar to bile acid sequestrants. Thus, specific aims of this pilot study were to orally administer clinoptilolite in different doses and granule size combinations to determine magnitude and time profile of changes in blood lipids...
June 12, 2017: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/28589411/accelerated-osteogenic-differentiation-of-human-bone-derived-cells-in-ankylosing-spondylitis
#10
Sungsin Jo, Suman Kang, Jinil Han, Seung Hyun Choi, Ye-Soo Park, Il-Hoon Sung, Tae-Hwan Kim
Ankylosing spondylitis (AS) is characterized by excessive bone formation with syndesmophytes, leading to bony ankylosis. The contribution of osteoblasts to the pathogenesis of ankylosis is poorly understood. The aim of this study was to determine molecular differences between disease controls (Ct) and AS bone-derived cells (BdCs) during osteogenic differentiation with or without inflammation using AS patient serum. We confirmed osteoblastic differentiation of Ct and AS BdCs under osteogenic medium by observing morphological changes and measuring osteoblastic differentiation markers...
June 6, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/28584575/melanin-extract-from-gallus-gallus-domesticus-promotes-proliferation-and-differentiation-of-osteoblastic-mg-63-cells-via-bone-morphogenetic-protein-2-signaling
#11
Han-Seok Yoo, Kang-Hyun Chung, Kwon-Jai Lee, Dong-Hee Kim, Jeung Hee An
BACKGROUND/OBJECTIVES: Gallus gallus domesticus (GD) is a natural mutant breed of chicken in Korea with an atypical characterization of melanin in its tissue. This study investigated the effects of melanin extracts of GD on osteoblast differentiation and inhibition of osteoclast formation. MATERIALS/METHODS: The effects of the melanin extract of GD on human osteoblast MG-63 cell differentiation were examined by evaluating cell viability, osteoblast differentiation, and expression of osteoblast-specific transcription factors such as bone morphogenetic protein 2 (BMP-2), small mothers against decapentaplegic homologs 5 (SMAD5), runt-related transcription factor 2 (RUNX2), osteocalcin and type 1 collagen (COL-1) by reverse transcription-polymerase chain reaction and western blotting analysis...
June 2017: Nutrition Research and Practice
https://www.readbyqxmd.com/read/28574653/cortical-and-trabecular-bone-microstructure-did-not-recover-at-weight-bearing-skeletal-sites-and-progressively-deteriorated-at-non-weight-bearing-sites-during-the-year-following-international-space-station-missions
#12
Laurence Vico, Bert Van Rietbergen, Nicolas Vilayphiou, Marie-Thérèse Linossier, Hervé Locrelle, Myriam Normand, Mohamed Zouch, Maude Gerbaix, Nicolas Bonnet, Valery Novikov, Thierry ThomC, Galina Vassilieva
Risk for premature osteoporosis is a major health concern in astronauts and cosmonauts and the reversibility of the bone lost at the weight-bearing bone sites is not established although it is suspected to take longer than the mission length. The bone three-dimensional structure and strength which could be uniquely affected by weightlessness, is currently unknown. Our objective is to evaluate bone mass, microarchitecture and strength of weight- and non-weight-bearing bone in 13 cosmonauts before and for 12 months after 4-6-month sojourn in the International Space Station (ISS)...
June 2, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28568134/lysyl-oxidase-mediated-vegf-induced-differentiation-and-angiogenesis-in-human-dental-pulp-cells
#13
W J Bae, J K Yi, J Park, S K Kang, J H Jang, E C Kim
AIM: To investigate the effects of recombinant human vascular endothelial growth factor (rhVEGF) on odontoblastic differentiation, in vitro angiogenesis, and expression and activity of lysyl oxidase (LOX) in human dental pulp cells (HDPCs), compared with rhFGF-2. To identify the underlying molecular mechanisms, the study focused on whether LOX was responsible for the actions of rhVEGF. METHODOLOGY: rhVEGF was constructed using the pBAD-HisA plasmid in Escherichia coli...
June 1, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28560407/tensile-strength-suppresses-the-osteogenesis-of-periodontal-ligament-cells-in-inflammatory-microenvironments
#14
Chaofan Sun, Fen Liu, Shendan Cen, Lijiao Chen, Yi Wang, Hao Sun, Hui Deng, Rongdang Hu
The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL‑1β and 10 ng/ml TNF‑α) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength‑induced cytokine expression were assessed by MTT assay and ELISA, respectively...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28559988/mir-31-is-involved-in-the-high-glucose-suppressed-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-satb2
#15
Lei Zhen, Xuewei Jiang, Yan Chen, Desheng Fan
Diabetes mellitus (DM) is a chronic metabolic disease that gives rise to impaired bone remodeling. Increasing evidences have shown that miRNAs are associated with osteogenic differentiation of stem cells. However, the underlying mechanism that links DM-induced HG conditions and impaired osteogenic differentiation capacity of periodontal ligament stem cells (PDLSCs) still remains unclear. In this study, we found that diabetic mice with increased miR-31 level in periodontal ligaments exhibited greater bone loss...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28558631/ursolic-acid-mediates-hepatic-protection-through-enhancing-of-anti-aging-biomarkers
#16
Shadi Gharibi, Nuredin Bakhtiari, Elham-Moslemee Jalalvand
BACKGROUND: Age-associated loss of liver function has been recognized for decades. But, the mechanism driving liver regeneration and its decline with age remains elusive. OBJECTIVE: Hence, to support of our previous studies about anti-aging effects of Ursolic Acid (UA), a compound which extensively present in apple peels. The aim of this study is to address whether UA might alter sensors of the cell metabolic state such as SIRT1, SIRT6, PGC-1β and Klotho proteins...
May 30, 2017: Current Aging Science
https://www.readbyqxmd.com/read/28552105/acacia-karroo-hayne-ethnomedicinal-uses-phytochemistry-and-pharmacology-of-an-important-medicinal-plant-in-southern-africa
#17
REVIEW
Alfred Maroyi
Acacia karroo (A. karroo) has been used as herbal medicine by the indigenous people of southern Africa for several centuries. The potential of A. karroo as herbal medicine, its associated phytochemistry and biological activities are reviewed. The extensive literature survey revealed that A. karroo is traditionally used to treat or manage 32 and five human and animal diseases and ailments, respectively. The species is used as herbal medicine for diseases and ailments such as colds, diarrhoea, dysentery, flu, malaria, sexually transmitted infections (STIs), wounds, and also as colic and ethnoveterinary medicine...
April 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28542442/pedogenetic-processes-in-anthrosols-with-pretic-horizon-amazonian-dark-earth-in-central-amazon-brazil
#18
Rodrigo S Macedo, Wenceslau G Teixeira, Marcelo M Corrêa, Gilvan C Martins, Pablo Vidal-Torrado
Anthrosols known as Amazonian Dark Earth (ADE) have borne witness to the intensification of sedentary patterns and the demographic increase in Central Amazon. As a result, a recurring pattern has been observed of mounds with ADE arising from domestic activities and the disposal of waste. The objective of this research was to demonstrate the relationship of these anthropic activities with pedogenetic formation processes of ADE in the municipality of Iranduba, Brazil. Disturbed and undisturbed soil samples were taken from two areas of ADE (pretic horizon) and from a non-anthropic pedon...
2017: PloS One
https://www.readbyqxmd.com/read/28541092/amniotic-mesenchymal-stromal-cells-exhibit-preferential-osteogenic-and-chondrogenic-differentiation-and-enhanced-matrix-production-compared-with-adipose-mesenchymal-stromal-cells
#19
Natasha Topoluk, Richard Hawkins, John Tokish, Jeremy Mercuri
BACKGROUND: Therapeutic efficacy of various mesenchymal stromal cell (MSC) types for orthopaedic applications is currently being investigated. While the concept of MSC therapy is well grounded in the basic science of healing and regeneration, little is known about individual MSC populations in terms of their propensity to promote the repair and/or regeneration of specific musculoskeletal tissues. Two promising MSC sources, adipose and amnion, have each demonstrated differentiation and extracellular matrix (ECM) production in the setting of musculoskeletal tissue regeneration...
May 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28538496/committee-opinion-no-702-female-athlete-triad
#20
(no author information available yet)
The female athlete triad is a medical condition observed in physically active females involving three components: 1) low energy availability with or without disordered eating, 2) menstrual dysfunction, and 3) low bone density. An individual does not need to show clinical manifestations of all three components of the female athlete triad simultaneously to be affected by the condition. Consequences of these clinical conditions may not be completely reversible, so prevention, early diagnosis, and intervention are critical...
June 2017: Obstetrics and Gynecology
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