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https://www.readbyqxmd.com/read/28324211/comparison-of-in-vitro-osteogenic-potential-of-iliac-crest-and-degenerative-facet-joint-bone-autografts-for-intervertebral-fusion-in-lumbar-spinal-stenosis
#1
Jeroen Geurts, Daniela Ramp, Stefan Schären, Cordula Netzer
PURPOSE: The promotion of spinal fusion using bone autografts is largely mediated by the osteoinductive potential of progenitors/mesenchymal stem cells (MSC) that reside in the marrow spaces of cancellous bone. Iliac crest is the common autograft donor site, but its use presents an increased risk for donor site pain, morbidity and infection. Degenerative bone samples harvested during facetectomy might provide an alternative viable source of osteoinductive autografts. In this study, we conducted an intra-individual comparison of the osteogenic potential of isolated low passage MSC from both sources...
March 21, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28324131/case-report-fast-reversal-of-severe-osteoporosis-after-correction-of-excessive-levothyroxine-treatment-and-long-term-follow-up
#2
C M Laine, K Landin-Wilhelmsen
This case report describes a 38-year-old woman, who presented with bilateral femoral stress fractures and osteoporosis after years of excessive levothyroxine treatment. Her bone health was restored rapidly and long-lasting with the reduction of levothyroxine dosage. No bone-active treatment was warranted. INTRODUCTION: Hyperthyroidism is a known risk factor for osteoporosis and fractures. Recent studies on patients with serum thyrotropin-suppressive therapy have not, however, indicated adverse effects on bone during long-term follow-up...
March 21, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28320084/lncrna-meg3-inhibited-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-from-postmenopausal-osteoporosis-by-targeting-mir-133a-3p
#3
Qiujun Wang, Ying Li, Yuanxia Zhang, Lan Ma, Lin Lin, Jia Meng, Lihong Jiang, Liping Wang, Ping Zhou, Yina Zhang
BACKGROUND AND AIMS: Long non-coding RNA (lncRNA) MEG3 has proven to be an important regulator involved in the pathogenesis and development of various human diseases. However, the functional involvement of MEG3 in postmenopausal osteoporosis (PMOP) and its mechanism is still unclear. METHODS: Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured from mouse pathologic models and patients with PMOP, respectively. The expression of MEG3 and miR-133a-3p in BMSCs was detected using qRT-PCR...
March 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28299231/chronic-osteoporotic-pain-in-mice-cutaneous-and-deep-musculoskeletal-pain-are-partially-independent-of-bone-resorption-and-differentially-sensitive-to-pharmacological-interventions
#4
Miyako Suzuki, Magali Millecamps, Lina Naso, Seiji Ohtori, Chisato Mori, Laura S Stone
Although the pathological changes in osteoporotic bones are well established, the characterization of the osteoporotic pain and its appropriate treatment are not fully elucidated. We investigated the behavioral signs of cutaneous and deep musculoskeletal pain and physical function; time-dependent changes in bone mineral density (BMD) and the emergence of the behavioral phenotype; and the effects of pharmacological interventions having different mechanisms of action (chronic intraperitoneal administration of pamidronate [0...
2017: Journal of Osteoporosis
https://www.readbyqxmd.com/read/28296593/protocatechuic-acid-enhances-osteogenesis-but-inhibits-adipogenesis-in-c3h10t1-2-and-3t3-l1-cells
#5
Adriana Rivera-Piza, Young Jae An, Do Kyung Kim, Sung-Hyen Lee, Jung-Bong Kim, Jung-Sook Choi, Sung-Joon Lee
Abnormal activation of adipogenesis in mesenchymal stem cells (MSCs) and preadipocyte cells is associated with human metabolic disorders, such as osteoporosis and obesity. This study investigated the biological effects of protocatechuic acid (PCA) on the modulation of osteogenesis and adipogenesis in cultured cells. PCA stimulation of MSCs significantly increased intracellular mineralization during osteogenesis, but reduced lipid accumulation in both MSCs and 3T3-L1 preadipocyte cells during adipogenesis. Reverse transcription-polymerase chain reaction and immunoblotting analyses showed a dose-dependent upregulation of proosteogenic runt-related transcription factor 2 due to induction of β-catenin...
March 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28294540/traditional-chinese-medicine-bushen-jianpi-huoxue-decoction-prevents-diabetic-osteoporosis-in-rats-via-wnt-and-nuclear-factor-kappa-b-signaling-pathways
#6
Yongqing Zhang, Mingming Liu, Haisheng Li, Zetao Chen, Na Liang, Jianguo Xu, Xiaoli Zhang, Yihang Zhang
AIM: The aim of this study was to evaluate the therapeutic effect of a traditional Chinese medicine (TCM), Bushen-Jianpi-Huoxue decoction (BJHD), on diabetic osteoporosis (DOP) and the action mechanisms likely mediated by nuclear factor-kappa B (NF-κB) and Wnt signaling pathways. METHODS: Fifty-five male Wistar rats were used in this study; they were divided into normal control (n = 10) and established DOP model (n = 45) groups. The DOP model was induced using a combination high carbohydrate - high fat diet and intraperitoneal injections of streptozotocin (STZ)...
March 13, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28292604/anti-osteoclastogenesis-of-mineral-trioxide-aggregate-through-inhibition-of-the-autophagic-pathway
#7
Xue Cheng, Lingxin Zhu, Jie Zhang, Jingjing Yu, Shan Liu, Fengyuan Lv, Ying Lin, Guojing Liu, Bin Peng
INTRODUCTION: Mineral trioxide aggregate (MTA) regulates bone remodeling, particularly osteoclast differentiation. However, intracellular mechanisms underlying the anti-osteoclastogenesis of MTA remain unclear. This study aimed to evaluate the potential alterations of autophagic pathway during anti-osteoclastogenic effects by MTA in vitro and investigate their underlying mechanisms. METHODS: Osteoclast precursors were treated with MTA extracts containing the receptor activator of nuclear factor-kappa B ligand (RANKL)...
March 11, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28272164/long-term-96-week-efficacy-and-safety-after-switching-from-tenofovir-disoproxil-fumarate-tdf-to-tenofovir-alafenamide-taf-in-hiv-infected-virologically-suppressed-adults
#8
Francois Raffi, Chloe Orkin, Amanda Clarke, Laurence Slama, Joel Gallant, Eric Daar, Keith Henry, Jorge Santana-Bagur, David K Stein, Nicholaos Bellos, Anthony Scarsella, Mingjin Yan, Michael E Abram, Andrew Cheng, Martin S Rhee
In a double-blind, phase 3 trial, 663 HIV-infected, virologically suppressed adults were randomized to switch to tenofovir alafenamide (TAF; n=333) vs. remain on tenofovir disoproxil fumarate (TDF; n=330), each coformulated with emtricitabine (FTC), while continuing their third agent (boosted protease inhibitor or unboosted third agent). At week 96, 88.6% on FTC/TAF and 89.1% on FTC/TDF had HIV-1 RNA <50 copies/mL (adjusted difference -0.5%; 95%CI [-5.3%, 4.4%]). Proteinuria, albuminuria, proximal renal tubular function, and bone mineral density improved after switching to TAF- from TDF-containing regimens...
March 6, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28259940/dickkopf-related-protein-3-negatively-regulates-the-osteogenic-differentiation-of-rat-dental-follicle-cells
#9
Xinchun Zhang, Yu Du, Junqi Ling, Weiqiang Li, Yan Liao, Xi Wei
The present study aimed to investigate the effect of Dickkopf-related protein 3 (DKK3) on osteogenic differentiation of rat dental follicle cells (DFCs). A PCR array analysis of Wnt pathway activation in DFCs identified genes dysregulated by mineral induction. Among them, DKK3expression levels were decreased, and further experiments were conducted to investigate its role in DFC osteogenesis. By comparing DFCs grown in normal growth and mineral‑induction media for 4 weeks, the present study confirmed that DKK3 was a potential target gene of osteogenesis through reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) and western blotting (WB)...
February 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28256004/vitamin-d-binding-protein-expression-in-healthy-tooth-and-periodontium-an-experimental-study-both-in-monkeys-in-vivo-and-in-humans-in-vitro
#10
W Li, W Zhu, J Hou, H Meng
BACKGROUND AND OBJECTIVE: Vitamin D-binding protein (DBP) is a highly expressed plasma protein with many important functions, including transport of vitamin D metabolites, sequestration of actin, control of bone metabolism and modulation of immune and inflammatory responses. Previous results of our study indicated an association between DBP and periodontitis. We hypothesized that periodontium might be another source of DBP in gingival crevicular fluid other than serum. MATERIAL AND METHODS: DBP expression was examined in dental and periodontal tissues of monkeys by immunohistochemistry, and in primary cells isolated from human dental and periodontal tissues by reverse transcription plus the polymerase chain reaction and immunocytochemistry...
March 2, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28218918/transgenic-expression-of-human-apol1-risk-variants-in-podocytes-induces-kidney-disease-in-mice
#11
Pazit Beckerman, Jing Bi-Karchin, Ae Seo Deok Park, Chengxiang Qiu, Patrick D Dummer, Irfana Soomro, Carine M Boustany-Kari, Steven S Pullen, Jeffrey H Miner, Chien-An A Hu, Tibor Rohacs, Kazunori Inoue, Shuta Ishibe, Moin A Saleem, Matthew B Palmer, Ana Maria Cuervo, Jeffrey B Kopp, Katalin Susztak
African Americans have a heightened risk of developing chronic and end-stage kidney disease, an association that is largely attributed to two common genetic variants, termed G1 and G2, in the APOL1 gene. Direct evidence demonstrating that these APOL1 risk alleles are pathogenic is still lacking because the APOL1 gene is present in only some primates and humans; thus it has been challenging to demonstrate experimental proof of causality of these risk alleles for renal disease. Here we generated mice with podocyte-specific inducible expression of the APOL1 reference allele (termed G0) or each of the risk-conferring alleles (G1 or G2)...
February 20, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28211947/insulin-like-growth-factor-1-promotes-osteogenic-differentiation-and-collagen-i-alpha-2-synthesis-via-induction-of-mrna-binding-protein-larp6-expression
#12
Yue Guo, Chen-Yi Tang, Xiao-Fei Man, Hao-Neng Tang, Jun Tang, Ci-La Zhou, Shu-Wen Tan, Min Wang, Yun-Zhi Feng, Hou-De Zhou
This study explored the mechanism underlying the stimulation of collagen synthesis and osteoblastic differentiation by insulin-like growth factor 1 (IGF1) in primary mouse osteoblasts. Primary mouse calvarial osteoblasts were cultured and treated with various doses of IGF1 before transfection with siRNA targeting the collagen type I alpha 2 (Col1a2) or La ribonucleoprotein domain family member 6 (Larp6) genes. Alkaline phosphatase (ALP) activity, osteocalcin staining, alizarin red quantification and the expression level of runt-related transcription factor 2 (RUNX2) were performed to assess the differentiation of pre-osteoblasts...
February 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28205268/the-effect-of-delta-like-1-homologue-on-the-proliferation-and-odontoblastic-differentiation-in-human-dental-pulp-stem-cells
#13
Shengcai Qi, Yanhong Yan, Yue Wen, Jialiang Li, Jing Wang, Fubo Chen, Xiaoshan Tang, Guangwei Shang, Yuanzhi Xu, Raorao Wang
INTRODUCTION: This study aimed to investigate the functions of delta-like homologue 1 (DLK1) in the proliferation and differentiation of human dental pulp stem cells (hDPSCs). METHODS: Immunohistochemical analysis was used to determine the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), DLK1, NOTCH1 and p-ERK1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs. The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay respectively...
February 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28197984/quantifying-bacterial-attachment-and-detachment-using-leaching-solutions-of-various-ionic-strengths-after-bacterial-pulse
#14
Nag-Choul Choi, Jae-Woo Choi, Kyu-Sang Kwon, Sang-Gil Lee, Soonjae Lee
In this study, we quantified the attachment and detachment of bacteria during transport in order to elucidate the contributions of reversible attachment on bacterial breakthrough curves. The first set of breakthrough experiment was performed for a laboratory sand column using leaching solutions of deionized water and mineral salt medium (MSM) of 200 mM with reference to KCl solution by employing Pseudomonas putida as a model bacterium. In the second set of experiment, the ionic strengths of leaching solutions immediately after bacterial pulse were lowered to tenfold and 100-fold diluted system (2 and 20 mM MSM) to focus on the influence of physicochemical factor...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28182625/viability-of-primary-osteoblasts-after-treatment-with-tenofovir-alafenamide-lack-of-cytotoxicity-at-clinically-relevant-drug-concentrations
#15
Christian Callebaut, Yang Liu, Darius Babusis, Adrian Ray, Michael Miller, Kathryn Kitrinos
Tenofovir alafenamide (TAF) is a phosphonoamidate prodrug of the nucleotide HIV reverse transcriptase inhibitor tenofovir (TFV). TAF is approved for the treatment of HIV-1 infection as part of the single-tablet regimen containing elvitegravir, cobicistat, emtricitabine, and TAF. When dosed once-daily, TAF results in approximately 90% lower levels of plasma TFV and a 4-fold increase in intracellular TFV-diphosphate (TFV-DP) in PBMCs compared with the TFV prodrug tenofovir disoproxil fumarate (TDF). Several antiretrovirals, including TDF, have been associated with bone mineral density decreases in patients; the effect of clinically relevant TAF concentrations on primary osteoblast viability was therefore assessed in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28178186/exogenous-pthrp-repairs-the-damaged-fracture-healing-of-pthrp-mice-and-accelerates-fracture-healing-of-wild-mice
#16
Yinhe Wang, Xin Fang, Chun Wang, Congzhu Ding, Hua Lin, Anlong Liu, Lei Wang, Yang Cao
Bone fracture healing is a complicated physiological regenerative process initiated in response to injury and is similar to bone development. To demonstrate whether an exogenous supply of parathyroid hormone-related protein (PTHrP) helps in bone fracture healing, closed mid-diaphyseal femur fractures were created and stabilized with intramedullary pins in eight-week-old wild-type (WT) PTHrP+/+ and PTHrP+/- mice. After administering PTHrP for two weeks, callus tissue properties were analyzed at one, two, and four weeks post-fracture (PF) by various methods...
February 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28169026/fluorescence-spectroscopy-and-parallel-factor-analysis-as-a-dissolved-organic-monitoring-tool-to-assess-treatment-performance-in-drinking-water-trains
#17
M Vera, S Cruz, M R Boleda, J Mesa, J Martín-Alonso, S Casas, O Gibert, J L Cortina
Fluorescence excitation emission matrix (FEEM) spectroscopy was used to evaluate its applicability as a tool to track dissolved organic matter (DOM) in a drinking water treatment plant (DWTP) that incorporates a conventional line (consisting in ozonation and GAC filtration) and a membrane-based line (consisting in ultrafiltration, reverse osmosis and mineralization) working in parallel. Seven sampling points within the different process stages were characterized monthly during 2014. A global Parallel Factor Analysis (PARAFAC) was used to pull out underlying organic fractions from the fluorescence spectra...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28168252/antiscaling-efficacy-of-caco3-and-caso4-on-polyethylene-glycol-peg-modified-reverse-osmosis-membranes-in-the-presence-of-humic-acid-interplay-of-membrane-surface-properties-and-water-chemistry
#18
Jessica R Ray, Whitney Wong, Young-Shin Jun
Mineral scaling persists in many water treatment processes. More specifically, it can significantly reduce the efficacy of aromatic polyamide (PA) membranes during reverse osmosis (RO) water treatment. Previous studies have integrated hydrophilic materials, such as polyethylene glycol (PEG), onto RO membranes to combat scaling from generally hydrophobic feed water constituents; however, there are still outstanding knowledge gaps regarding the interplay of the modified membrane surface chemistry and the water chemistry in complex RO feed waters...
February 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28142110/role-of-two-sided-crosstalk-between-no-and-h2s-on-improvement-of-mineral-homeostasis-and-antioxidative-defense-in-sesamum-indicum-under-lead-stress
#19
Rayhaneh Amooaghaie, Faezeh Zangene-Madar, Shekoofeh Enteshari
H2S and NO are two important gasotransmitters that modulate stress responses in plants. There are the contradictory data on crosstalk between NO and H2S in the studies. Hence, in the present study, the role of interplay between NO and H2S was assessed on the Pb tolerance of Sesamum indicum using pharmacological and biochemical approaches. Results revealed that Pb stress reduced the plant growth and the content of photosynthetic pigments and Fv/Fm ratio, increased the lipid peroxidation and the H2O2 content, elevated the endogenous contents of nitric oxide (NO), H2S and enhanced the activities of antioxidant enzymes (except APX)...
January 28, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28137405/contraception-for-adolescents-with-chronic-rheumatic-diseases
#20
Benito Lourenço, Katia T Kozu, Gabriela N Leal, Marco F Silva, Elisabeth G C Fernandes, Camila M P França, Fernando H C Souza, Clovis A Silva
Contraception is an important issue and should be a matter of concern in every medical visit of adolescent and young patients with chronic rheumatic diseases. This narrative review discusses contraception methods in adolescents with juvenile systemic lupus erythematosus (JSLE), antiphospholipid syndrome (APS), juvenile idiopathic arthritis (JIA) and juvenile dermatomyositis (JDM). Barrier methods are safe and their use should be encouraged for all adolescents with chronic rheumatic diseases. Combined oral contraceptives (COC) are strictly prohibited for JSLE and APS patients with positive antiphospholipid antibodies...
January 2017: Revista Brasileira de Reumatologia
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