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JOURNAL ARTICLE
Barbara Nunes Padovani, Camila Morales Fénero, Lais Cavalieri Paredes, Mariana Abrantes do Amaral, Omar Domínguez-Amorocho, Marcella Cipelli, Juliana Moreira Mendonça Gomes, Eloisa Martins da Silva, Luísa Menezes Silva, Raquel de Souza Vieira, Mariana Tominaga Pereira, Mario Costa Cruz, Niels Olsen Saraiva Câmara
Cisplatin is an antineoplastic agent used to treat various tumors. In mammals, it can cause nephrotoxicity, tissue damage, and inflammation. The release of inflammatory mediators leads to the recruitment and infiltration of immune cells, particularly neutrophils, at the site of inflammation. Cisplatin is often used as an inducer of acute kidney injury (AKI) in experimental models, including zebrafish ( Danio rerio ), due to its accumulation in kidney cells. Current protocols in larval zebrafish focus on studying its effect as an AKI inducer but ignore other systematic outcomes...
February 17, 2024: International Journal of Molecular Sciences
#22
Neranjan de Silva, Lauretta A Lacko, Edgar A Jamies, Todd Evans, Romulo Hurtado
3D reconstructive imaging is a powerful strategy to interrogate the global architecture of tissues. We developed Atacama Clear (ATC), a novel method that increases 3D imaging signal-to-noise ratios (SNRs) while simultaneously increasing the capacity of tissue to be cleared. ATC potentiated the clearing capacity of all tested chemical reagents currently used for optical clearing by an average of 68%, and more than doubled SNRs. This increased imaging efficacy enabled multiplex interrogation of tough fibrous tissue and specimens that naturally exhibit high levels of background noise, including the heart, kidney, and human biopsies...
January 23, 2024: bioRxiv
#23
Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles.
Sissy Bassani, Jacqueline Chrast, Giovanna Ambrosini, Norine Voisin, Frédéric Schütz, Alfredo Brusco, Fabio Sirchia, Lydia Turban, Susanna Schubert, Rami Abou Jamra, Jan-Ulrich Schlump, Desiree DeMille, Pinar Bayrak-Toydemir, Gary Rex Nelson, Kristen Nicole Wong, Laura Duncan, Mackenzie Mosera, Christian Gilissen, Lisenka E L M Vissers, Rolph Pfundt, Rogier Kersseboom, Hilde Yttervik, Geir Åsmund Myge Hansen, Marie Falkenberg Smeland, Kameryn M Butler, Michael J Lyons, Claudia M B Carvalho, Chaofan Zhang, James R Lupski, Lorraine Potocki, Leticia Flores-Gallegos, Rodrigo Morales-Toquero, Florence Petit, Binnaz Yalcin, Annabelle Tuttle, Houda Zghal Elloumi, Lane Mccormick, Mary Kukolich, Oliver Klaas, Judit Horvath, Marcello Scala, Michele Iacomino, Francesca Operto, Federico Zara, Karin Writzl, Ales Maver, Maria K Haanpää, Pia Pohjola, Harri Arikka, Christian Iseli, Nicolas Guex, Alexandre Reymond
BACKGROUND: We previously described the KINSSHIP syndrome, an autosomal dominant disorder associated with intellectual disability (ID), mesomelic dysplasia and horseshoe kidney,caused by de novo variants in the degron of AFF3. Mouse knock-ins and overexpression in zebrafish provided evidence for a dominant-negative (DN) mode-of-action, wherein an increased level of AFF3 resulted in pathological effects. METHODS: Evolutionary constraints suggest that other mode-of-inheritance could be at play...
January 17, 2024: medRxiv
#24
JOURNAL ARTICLE
Lora Petrie-Hanson, Ann E Beth Peterman
A variety of biological toxins can be present at harmful levels in the aquatic environment. Cyanobacteria are a diverse group of prokaryotic microorganisms that produce cyanotoxins in the aquatic environment. These biotoxins can be hepatotoxins, dermatoxins, or neurotoxins and can affect fish and mammals. At high levels, these compounds are fatal. At non-lethal levels, they act insidiously and affect immune cell functions. Algae-produced biotoxins include microcystin and anatoxin A. Aquatic animals can also ingest material contaminated with botulinum neurotoxin E (BoNT/E) produced by Clostridium botulinum, also resulting in death or decreased immune functions...
January 12, 2024: Journal of Visualized Experiments: JoVE
#25
JOURNAL ARTICLE
Sophia Baker, Thanh Khoa Nguyen, Rebecca A Wingert
Cilia are hair-like structures found on the surface of nearly all vertebrate cell types where they have central roles in regulating development and orchestrating physiological events. There is growing interest in understanding the mechanisms of ciliogenesis due to the profound consequences that follow from the absence of proper ciliary function, which include diseases that affect the renal, respiratory, reproductive, nervous, visual, and digestive systems, among others. Now, a recent report has discerned new roles for the transcription factor estrogen-related receptor gamma a ( esrrγa) in ciliated cell ontogeny within the embryonic zebrafish kidney and other tissues...
January 28, 2024: Tissue Barriers
#26
JOURNAL ARTICLE
Bo Li, Shan Nan Chen, Lin Huang, Li Li, Li Ren, Jing Hou, Jing Yun Tian, Lan Hao Liu, P Nie
Type II interferons (IFNs) are a key class of molecules regulating innate and adaptive immunity in vertebrates. In the present study, two members of the type II IFNs, IFN-γ and IFNγ-rel, were identified in the blunt snout bream (Megalobrama amblycephala). The open reading frame (ORF) of IFN-γ and IFNγ-rel was found to have 564 bp and 492 bp, encoding 187 and 163 amino acids, with the first 26 and 24 amino acids being the signal peptide, respectively. IFN-γ and IFNγ-rel genes showed a high degree of similarity to their zebrafish homologues, being 76...
January 26, 2024: Fish & Shellfish Immunology
#27
JOURNAL ARTICLE
Anna L Luss, Dmitry V Bagrov, Anne V Yagolovich, Ekaterina V Kukovyakina, Irina I Khan, Vadim S Pokrovsky, Maria V Shestovskaya, Marine E Gasparian, Dmitry A Dolgikh, Andrey N Kuskov
Curcumin attracts huge attention because of its biological properties: it is antiproliferative, antioxidant, anti-inflammatory, immunomodulatory and so on. However, its usage has been limited by poor water solubility and low bioavailability. Herein, to solve these problems, we developed curcumin-loaded nanoparticles based on end-capped amphiphilic poly(N-vinylpyrrolidone). Nanoparticles were obtained using the solvent evaporation method and were characterized by dynamic and electrophoretic light scattering, transmission electron (TEM) and atomic force (AFM) microscopy...
December 19, 2023: Pharmaceutics
#28
JOURNAL ARTICLE
Kyung-Hyun Cho, Ashutosh Bahuguna, Dae-Jin Kang, Ji-Eun Kim
Ozonated sunflower oil (OSO) is renowned for its diverse therapeutic benefits. Nonetheless, the consequences of extended dietary intake of OSO have yet to be thoroughly investigated. Herein, the effect of 2-year dietary supplementation of OSO was examined on the survivability, obesity, skeletal deformities, swimming behavior, and liver, kidney, ovary, and testis function of zebrafish. Results showed that the zebrafish feed supplemented with 20% ( wt / wt ) OSO for 2 years emerged with higher survivability and body weight management compared to sunflower oil (SO) and normal diet (ND)-supplemented zebrafish...
January 19, 2024: Antioxidants (Basel, Switzerland)
#29
JOURNAL ARTICLE
Yingying Wang, Huili Yao, Yu Zhang, Ning Mu, Tong Lu, Zhiyuan Du, Yingdi Wu, Xiaopeng Li, Min Su, Ming Shao, Xiaoyang Sun, Ling Su, Xiangguo Liu
Primary cilia are enriched in signaling receptors, and defects in their formation or function can induce conditions such as polycystic kidney disease, postaxial hexadactyly, and microphthalmia. Mammalian Hedgehog (Hh) signaling is important in the development of primary cilia, and TMEM216, a transmembrane protein that localizes to the base of cilia, is also implicated in ciliogenesis in zebrafish. Here, we found that Tmem216 -deficient mice had impaired Hh signaling and displayed typical ciliopathic phenotypes...
January 23, 2024: Science Signaling
#30
JOURNAL ARTICLE
Christopher B Mahony, Rui Monteiro
The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marrow cellularity, and analysis of cell populations by flow cytometry. We then detail procedures for May-Grünwald-Giemsa staining for analysis of cellular morphology and phenotyping. For complete details on the use and execution of this protocol, please refer to Mahony et al...
January 22, 2024: STAR protocols
#31
JOURNAL ARTICLE
Kyung-Hyun Cho, Ji-Eun Kim, Hyo-Seon Nam, Seung-Hee Baek, Ashutosh Bahuguna
The current study compared three policosanols from Cuba (sugarcane, Raydel® , policosanol (1), China (rice bran, Shaanxi, policosanol (2), and the USA (sugarcane, Lesstanol® , policosanol (3) in the treatment of dyslipidemia and protection of the liver, ovary, and testis in hypercholesterolemic zebrafish. After twelve weeks of supplementation of each policosanol (PCO, final 0.1% in diet, w / w ) with a high cholesterol diet (HCD, final 4%, w / w ), the Raydel policosanol (PCO1) group showed the highest survivability, approximately 89%...
December 30, 2023: Pharmaceuticals
#32
JOURNAL ARTICLE
Kyung-Hyun Cho, Seung-Hee Baek, Hyo-Seon Nam, Ashutosh Bahuguna, Luis Ernesto López-González, Iván Rodríguez-Cortina, José Illnait-Ferrer, Julio César Fernández-Travieso, Vivian Molina-Cuevas, Yohani Pérez-Guerra, Ambar Oyarzabal Yera, Sarahi Mendoza-Castaño
Oxidative stress is one of the primary instigators of the onset of various human ailments, including cancers, cardiovascular diseases, and dementia. Particularly, oxidative stress severely affects low-density lipid & protein (LDL) oxidation, leading to several detrimental health effects. Therefore, in this study, the effect of beeswax alcohol (BWA) was evaluated in the prevention of LDL oxidation, enhancement of paraoxonase 1 (PON-1) activity of high-density lipid & protein (HDL), and zebrafish embryo survivability...
January 2, 2024: Current Issues in Molecular Biology
#33
JOURNAL ARTICLE
Nicole Endlich
BACKGROUND: Focal segmental glomerulosclerosis (FSGS) is a rare disease, or damage to the filtering units of the kidney, the glomeruli, about of which there is only limited knowledge and few treatment options. The STOP-FSGS consortium has set itself the goal to expand our knowledge of this disease and develop new treatment options. PROJECT: Through intensive research and the use of state-of-the-art techniques such as super-resolution microscopy, AI-based imaging and single-cell research, the consortium aims to gain a deeper understanding of the mechanisms of FSGS...
December 2023: Journal of health monitoring
#34
JOURNAL ARTICLE
Feng Wang, Ruizhi Zhang, Jing Jian, Yanhe Sun, Qiang Li
INTRODUCTION: Branchio-oto-renal syndrome (BOR syndrome) is a rare genetic disorder with an incidence of 1 in 40,000, affecting the development of multiple organs, including the branchio, ear and kidney. It is responsible for 2% of childhood deafness. Currently, variants in the coding regions of the main causative genes, such as EYA1, SIX1, and SIX5, explain only half of the disease's etiology. Therefore, there is a need to explore the non-coding regions, which constitute the majority of the genome, especially the regulatory regions, as potential new causative factors...
January 16, 2024: Developmental Neuroscience
#35
JOURNAL ARTICLE
Pinar Goc Rasgele, Fatma Demir, Serife Gulsun Kirankaya
The aim of this study was to investigate the genotoxic effect of Propineb fungicide at different concentrations (0.167, 0.335 and 0.670 mg L-1 ) and different treatment times (24, 48, 72 and 96 h) on Danio rerio . At the end of the treatment periods, blood was collected from the fish with a heparin injector; smear preparations were prepared, fixed and stained. In the prepared preparations, the numbers of cells with MN and erythrocyte nucleus abnormalities were examined. It was found that propineb increased micronucleus formation at all treatment times and concentrations and induced the formation of erythrocytes with morphological abnormal nuclei such as segmented, kidney-shaped, notched, vacuolated nuclei and binucleated...
January 15, 2024: Drug and Chemical Toxicology
#36
JOURNAL ARTICLE
Xuemei Yu, Yuanyuan Wang, Zhenghua Wu, Mengqi Jia, Ying Xu, Han Qu, Xin Zhao, Shuowen Wang, Lili Jing, Yuefen Lou, Guorong Fan, Yaxing Gui
ETHNOPHARMACOLOGICAL RELEVANCE: According to the Compendium of Materia Medica (Shizhen Li, Ming dynasty) and Welfare Pharmacy (Song dynasty), Psoraleae Fructus (PF), a traditional Chinese medicine (TCM) has a bitter taste and warm nature, which has the effect of treating spleen and kidney deficiency and skin disease. Although PF has been widely used since ancient times and has shown satisfactory efficacy in treating vitiligo, the active substances and the mechanism of PF in promoting melanogenesis remain unclear...
January 11, 2024: Journal of Ethnopharmacology
#37
JOURNAL ARTICLE
Haowei Liang, Ruiqin Zhang, Li Zhou, Xiaolong Wu, Jingan Chen, Xinyue Li, Jieqiong Chen, Letian Shan, Hui Wang
ETHNOPHARMACOLOGICAL RELEVANCE: Cornstigma (CS), derived from the stigma and style of gramineous plant Zeamays. The medicinal use of CS can be traced back to DianNanMateriaMedica. LingnanMedicinalPlantsCompendium records its effectiveness in ameliorating diabetes. Diabetes is a metabolic disorder characterized by hyperglycemia and the consequent chronic complications of kidney, heart, brain and other organs, which pose a significant threat to human health. CS has shown great potential in relieving hyperglycemia associated with diabetes...
January 10, 2024: Journal of Ethnopharmacology
#38
JOURNAL ARTICLE
Luyuan Qin, Jiadong Huang, Yuanzhou Feng, Baoquan Zhao, Lei Guo, Jianwei Xie
Paraquat is a highly toxic quaternary ammonium herbicide. It can damage the functions of multiple organs and cause irreversible pulmonary fibrosis in the human body. However, the toxicological mechanism of paraquat is not yet fully understood, and due to the lack of specific antidotes, the clinical treatment of paraquat intoxication is still a great medical challenge. In-depth research on its toxicity mechanism, toxicokinetics, and effective antidotes is urgently demanded. A new molecular imaging technique, matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), can simultaneously achieve quantitative and spatial analysis and offer an alternative, distinct, and useful technique for paraquat intoxication and consequent detoxication...
January 11, 2024: Chemical Research in Toxicology
#39
REVIEW
Maximilian Schindler, Nicole Endlich
Podocytes, specialized postmitotic cells, are central players in various kidney-related diseases. Zebrafish have become a valuable model system for studying podocyte biology because they are genetically easy to manipulate, transparent and their glomerular structure is similar to that of mammals. This review provides an overview of the knowledge of podocyte biology in zebrafish larvae, with particular focus on their essential contribution to understanding the mechanisms that underlie kidney diseases as well as supporting drug development...
January 11, 2024: American Journal of Physiology. Renal Physiology
#40
Valentina Cipriani, Letizia Vestito, Emma F Magavern, Julius Ob Jacobsen, Gavin Arno, Elijah R Behr, Katherine A Benson, Marta Bertoli, Detlef Bockenhauer, Michael R Bowl, Kate Burley, Li F Chan, Patrick Chinnery, Peter Conlon, Marcos Costa, Alice E Davidson, Sally J Dawson, Elhussein Elhassan, Sarah E Flanagan, Marta Futema, Daniel P Gale, Sonia García-Ruiz, Cecilia Gonzalez Corcia, Helen R Griffin, Sophie Hambleton, Amy R Hicks, Henry Houlden, Richard S Houlston, Sarah A Howles, Robert Kleta, Iris Lekkerkerker, Siying Lin, Petra Liskova, Hannah Mitchison, Heba Morsy, Andrew D Mumford, William G Newman, Ruxandra Neatu, Edel A O'Toole, Albert Cm Ong, Alistair T Pagnamenta, Shamima Rahman, Neil Rajan, Peter N Robinson, Mina Ryten, Omid Sadeghi-Alavijeh, John A Sayer, Claire L Shovlin, Jenny C Taylor, Omri Teltsh, Ian Tomlinson, Arianna Tucci, Clare Turnbull, Albertien M van Eerde, James S Ware, Laura M Watts, Andrew R Webster, Sarah K Westbury, Sean L Zheng, Mark Caulfield, Damian Smedley
To discover rare disease-gene associations, we developed a gene burden analytical framework and applied it to rare, protein-coding variants from whole genome sequencing of 35,008 cases with rare diseases and their family members recruited to the 100,000 Genomes Project (100KGP). Following in silico triaging of the results, 88 novel associations were identified including 38 with existing experimental evidence. We have published the confirmation of one of these associations, hereditary ataxia with UCHL1 , and independent confirmatory evidence has recently been published for four more...
December 21, 2023: medRxiv
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