keyword
MENU ▼
Read by QxMD icon Read
search

zebrafish kidney

keyword
https://www.readbyqxmd.com/read/28411182/pegylation-rate-influences-peptide-based-nanoparticles-mediated-sirna-delivery-in-vitro-and-in-vivo
#1
Gudrun Aldrian, Anaïs Vaissière, Karidia Konate, Quentin Seisel, Eric Vivès, Frédéric Fernandez, Véronique Viguier, Coralie Genevois, Franck Couillaud, Héléne Démèné, Dina Aggad, Aurélie Covinhes, Stéphanie Barrère-Lemaire, Sébastien Deshayes, Prisca Boisguerin
Small interfering RNAs (siRNAs) present a strong therapeutic potential because of their ability to inhibit the expression of any desired protein. Recently, we developed the retro-inverso amphipathic RICK peptide as novel non-covalent siRNA carrier. This peptide is able to form nanoparticles (NPs) by self-assembling with the siRNA resulting in the fully siRNA protection based on its protease resistant peptide sequence. With regard to an in vivo application, we investigated here the influence of the polyethylene glycol (PEG) grafting to RICK NPs on their in vitro and in vivo siRNA delivery properties...
April 11, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28411024/epcam-controls-morphogenetic-programs-during-zebrafish-pronephros-development
#2
Sebastian Kuechlin, Maximilian Schoels, Krasimir Slanchev, Silke Lassmann, Gerd Walz, Toma A Yakulov
Epithelial cell adhesion molecule EpCAM is a transmembrane glycoprotein that is dynamically expressed in human and murine renal epithelia during development. The levels of EpCAM in the renal epithelium are upregulated both during regeneration after ischemia/reperfusion injury and in renal-derived carcinomas. The role of EpCAM in early kidney development, however, has remained unclear. The zebrafish pronephros shows a similar segmentation pattern to the mammalian metanephric nephron, and has recently emerged as a tractable model to study the regulatory programs governing early nephrogenesis...
April 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28409342/zebrafish-as-a-model-of-kidney-disease
#3
Elvin E Morales, Rebecca A Wingert
Animal models have been an invaluable means to advance biomedical research as they provide experimental avenues for cellular and molecular investigations of disease pathology. The zebrafish (Danio rerio) is a good alternative to mammalian models that can be used to apply powerful genetic experimental methods normally used in invertebrates to answer questions about vertebrate development and disease. In the case of the kidney, the zebrafish has proven itself to be an applicable and versatile experimental system, mainly due to the simplicity of its pronephros, which contains two nephrons that possess conserved structural and physiological aspects with mammalian nephrons...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/28409341/zebrafish-pronephros-development
#4
Richard W Naylor, Sarah S Qubisi, Alan J Davidson
The pronephros is the first kidney type to form in vertebrate embryos. The first step of pronephrogenesis in the zebrafish is the formation of the intermediate mesoderm during gastrulation, which occurs in response to secreted morphogens such as BMPs and Nodals. Patterning of the intermediate mesoderm into proximal and distal cell fates is induced by retinoic acid signaling with downstream transcription factors including wt1a, pax2a, pax8, hnf1b, sim1a, mecom, and irx3b. In the anterior intermediate mesoderm, progenitors of the glomerular blood filter migrate and fuse at the midline and recruit a blood supply...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/28363126/interaction-between-the-zebrafish-danio-rerio-organic-cation-transporter-1-oct1-and-endo-and-xenobiotics
#5
Ivan Mihaljević, Marta Popović, Roko Žaja, Nikola Maraković, Goran Šinko, Tvrtko Smital
Organic cation transporters (OCTs) serve as uptake transporters of numerous endo- and xenobiotics. They have been in the focus of medical toxicological research for more than a decade due to their key role in absorption, distribution, metabolism and excretion due to their expression on basolateral membranes of various barrier tissues. OCTs belong to the SLC22A family within the SLC (Solute carrier) protein superfamily, with three co-orthologs identified in humans (OCT1, 2 and 3), and two Oct orthologs in zebrafish (Oct1 and Oct2)...
March 18, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28360383/cloning-and-expression-analysis-of-zygote-arrest-1-zar1-in-new-zealand-white-rabbits
#6
Dan Wang, Shu-Yu Xie, Wei Zhang, Cai-Xia Sun, Tao Huang, An-Si Wang, Xue-Lei Han, Gui-Rong Sun, Ming Li
Zygote arrest 1 (Zar1) is an oocyte-specific maternal-effect gene. Previous studies indicate that Zar1 plays important role in early embryo development, but little is known about its function in rabbit. The objectives of this study were to clone the New Zealand white rabbit Zar1 gene and to investigate its expression in various organs in groups of animals with different reproductive traits.We obtained a 709-bp Zar1 cDNA fragment consisting of an 8-bp exon 1, 161-bp exon 2, 75-bp exon 3, 271-bp exon 4 and 194-bp 3'sequences...
March 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28359809/wnt8a-expands-the-pool-of-embryonic-kidney-progenitors-in-zebrafish
#7
Richard W Naylor, Hwa In Han, Neil A Hukriede, Alan J Davidson
During zebrafish embryogenesis the pronephric kidney arises from a small population of posterior mesoderm cells that then undergo expansion during early stages of renal organogenesis. While wnt8 is required for posterior mesoderm formation during gastrulation, it is also transiently expressed in the post-gastrula embryo in the intermediate mesoderm, the precursor to the pronephros and some blood/vascular lineages. Here, we show that knockdown of wnt8a, using a low dose of morpholino that does not disrupt early mesoderm patterning, reduces the number of kidney and blood cells...
March 27, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28356434/the-growth-promoting-effect-of-dietary-nucleotides-in-fish-is-associated-with-an-intestinal-microbiota-mediated-reduction-in-energy-expenditure
#8
Xiaoze Guo, Chao Ran, Zhen Zhang, Suxu He, Min Jin, Zhigang Zhou
Background: Nucleotides have been used as functional nutrients to improve the growth and health of animals, including fish. The mechanism involved in the growth-promotion effect of nucleotides is still unclear.Objective: We investigated the bioenergetic mechanism underlying the growth-promotion effect of nucleotides in zebrafish and the associated roles played by the intestinal microbiota.Methods: Larval zebrafish were fed a control or a 0.1% mixed nucleotides-supplemented diet for 2 wk. Standard metabolic rate, the minimal rate of energy expenditure by animals at rest, was evaluated by oxygen consumption with the use of a respirometer...
March 29, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28335864/using-zebrafish-to-study-kidney-development-and-disease
#9
Stephanie Jerman, Zhaoxia Sun
The kidneys are a crucial pair of organs that are responsible for filtering the blood to remove waste, maintain electrolyte and water homeostasis, and regulate blood pressure. There are a number of factors, both genetic and environmental, that can impair the function of the kidneys resulting in significant morbidity and mortality for millions of people affected by kidney disease worldwide. The zebrafish, Danio rerio, has emerged as an attractive vertebrate model in the study of kidney development and disease and has proven to be a powerful tool in the advancement of how kidney development occurs in vertebrates and how the kidney repairs itself after injury...
2017: Current Topics in Developmental Biology
https://www.readbyqxmd.com/read/28331970/disease-modeling-in-genetic-kidney-diseases-zebrafish
#10
REVIEW
Heiko Schenk, Janina Müller-Deile, Mark Kinast, Mario Schiffer
Growing numbers of translational genomics studies are based on the highly efficient and versatile zebrafish (Danio rerio) vertebrate model. The increasing types of zebrafish models have improved our understanding of inherited kidney diseases, since they not only display pathophysiological changes but also give us the opportunity to develop and test novel treatment options in a high-throughput manner. New paradigms in inherited kidney diseases have been developed on the basis of the distinct genome conservation of approximately 70 % between zebrafish and humans in terms of existing gene orthologs...
March 22, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28316899/water-quality-assessment-in-the-german-river-of-the-years-2014-2015-how-a-case-study-on-the-impact-of-a-storm-water-sedimentation-basin-displayed-impairment-of-fish-health-in-the-argen-river-southern-germany
#11
Paul Thellmann, Bertram Kuch, Karl Wurm, Heinz-R Köhler, Rita Triebskorn
BACKGROUND: The present work investigates the impact of discharges from a storm water sedimentation basin (SSB) receiving runoff from a connected motorway in southern Germany. The study lasted for almost two years and was aimed at assessing the impact of the SSB on the fauna of the Argen River, which is a tributary of Lake Constance. Two sampling sites were examined up- and downstream of the SSB effluent. A combination of different diagnostic methods (fish embryo test with the zebrafish, histopathology, micronucleus test) was applied to investigate health impairment and genotoxic effects in indigenous fish as well as embryotoxic potentials in surface water and sediment samples of the Argen River, respectively, in samples of the SSB effluent...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28290603/fenofibrate-exerts-protective-effects-against-gentamicin-induced-toxicity-in-cochlear-hair-cells-by-activating-antioxidant-enzymes
#12
Channy Park, Hye-Min Ji, Se-Jin Kim, Sung-Hee Kil, Joon No Lee, Seongae Kwak, Seong-Kyu Choe, Raekil Park
Fenofibrate, an activator of peroxisome proliferator-activated receptors (PPARs), has been shown to protect the kidneys and brain cells from oxidative stress; however, its role in preventing hearing loss has not been reported to date, at least to the best of our knowledge. In this study, we demonstrated the protective effects of fenofibrate against gentamicin (GM)-induced ototoxicity. We found that the auditory brainstem response threshold which was increased by GM was significantly reduced by pre-treatment with fenofibrate in rats...
April 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28281657/a-gcsfr-csf3r-zebrafish-mutant-models-the-persistent-basal-neutrophil-deficiency-of-severe-congenital-neutropenia
#13
Vahid Pazhakh, Sharon Clark, M Cristina Keightley, Graham J Lieschke
Granulocyte colony-stimulating factor (GCSF) and its receptor (GCSFR), also known as CSF3 and CSF3R, are required to maintain normal neutrophil numbers during basal and emergency granulopoiesis in humans, mice and zebrafish. Previous studies identified two zebrafish CSF3 ligands and a single CSF3 receptor. Transient antisense morpholino oligonucleotide knockdown of both these ligands and receptor reduces neutrophil numbers in zebrafish embryos, a technique widely used to evaluate neutrophil contributions to models of infection, inflammation and regeneration...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28271061/identification-and-expression-analysis-of-the-complete-family-of-zebrafish-pkd-genes
#14
Samantha J England, Paul C Campbell, Santanu Banerjee, Annika J Swanson, Katharine E Lewis
Polycystic kidney disease (PKD) proteins are trans-membrane proteins that have crucial roles in many aspects of vertebrate development and physiology, including the development of many organs as well as left-right patterning and taste. They can be divided into structurally-distinct PKD1-like and PKD2-like proteins and usually one PKD1-like protein forms a heteromeric polycystin complex with a PKD2-like protein. For example, PKD1 forms a complex with PKD2 and mutations in either of these proteins cause Autosomal Dominant Polycystic Kidney Disease (ADPKD), which is the most frequent potentially-lethal single-gene disorder in humans...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28262634/francisella-noatunensis-ssp-noatunensis-iglc-deletion-mutant-protects-adult-zebrafish-challenged-with-acute-mortality-dose-of-wild-type-strain
#15
Elisabeth O Lampe, Julia I Tandberg, Anne-Lise Rishovd, Hanne C Winther-Larsen
The intracellular fish pathogen Francisella noatunensis remains an unsolved problem for aquaculture worldwide and an efficient vaccine is needed. In Francisella sp., IglC is an important virulence factor necessary for intracellular growth and escape from phagolysosomes. Deletion of the intracellular growth locus C (iglC) in Francisella sp. causes attenuation, but vaccine potential has only been attributed to ΔiglC from Francisella noatunensis ssp. orientalis, a warm-water fish pathogen. A ΔiglC mutant was constructed in the cold-water fish pathogen F...
March 6, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28252672/acute-podocyte-injury-is-not-a-stimulus-for-podocytes-to-migrate-along-the-glomerular-basement-membrane-in-zebrafish-larvae
#16
Florian Siegerist, Antje Blumenthal, Weibin Zhou, Karlhans Endlich, Nicole Endlich
Podocytes have a unique 3D structure of major and interdigitating foot processes which is the prerequisite for renal blood filtration. Loss of podocytes leads to chronic kidney disease ending in end stage renal disease. Until now, the question if podocytes can be replaced by immigration of cells along the glomerular basement membrane (GBM) is under debate. We recently showed that in contrast to former theories, podocytes are stationary in the zebrafish pronephros and neither migrate nor change their branching pattern of major processes over 23 hours...
March 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28247754/small-sized-mpeg-plga-nanoparticles-of-schisantherin-a-with-sustained-release-for-enhanced-brain-uptake-and-anti-parkinsonian-activity
#17
Tongkai Chen, Chuwen Li, Ye Li, Xiang Yi, Ruibing Wang, Simon Ming-Yuen Lee, Ying Zheng
Schisantherin A (SA) is a promising anti-Parkinsonism natural product. However, its poor water solubility and rapid serum clearance impose significant barriers to delivery of SA to the brain. This work aimed to develop SA in a nanoparticle formulation that extended SA circulation in the bloodstream and consequently an increased brain uptake and thus to be potentially efficacious for the treatment of Parkinson's disease (PD). Spherical SA nanoparticles with a mean particle size of 70 nm were prepared by encapsulating SA into methoxy poly(ethylene glycol)-block-poly(d,l)-lactic-co-glycolic acid (mPEG-PLGA) nanoparticles (SA-NPs) with an encapsulation efficiency of ∼91% and drug loading of ∼28%...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28228401/wolf-hirschhorn-syndrome-candidate-1-like-1-epigenetically-regulates-nephrin-gene-expression
#18
Yugo Ito, Kan Katayama, Yukino Nishibori, Yoshihiro Akimoto, Akihiko Kudo, Ryota Kurayama, Ichiro Hada, Shohei Takahashi, Toru Kimura, Toshiyuki Fukutomi, Tomohisa Katada, Junichi Suehiro, Olga Beltcheva, Karl Tryggvason, Kunimasa Yan
Altered expression of nephrin underlies the pathophysiology of proteinuria in both congenital and acquired nephrotic syndrome. However, the epigenetic mechanisms of nephrin gene regulation remain elusive. Here, we show that Wolf-Hirschhorn syndrome candidate 1-like 1 long form (WHSC1L1-L) is a novel epigenetic modifier of nephrin gene regulation. WHSC1L1-L was associated with histone H3K4 and H3K36 in human embryonic kidney cells. WHSC1L1-L gene was expressed in the podocytes and functional protein product was detected in these cells...
February 22, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28220184/cxcl12a-cxcr4b-acts-to-retain-neutrophils-in-caudal-hematopoietic-tissue-and-to-antagonize-recruitment-to-an-injury-site-in-the-zebrafish-larva
#19
Susana Paredes-Zúñiga, Rodrigo A Morales, Salomé Muñoz-Sánchez, Carlos Muñoz-Montecinos, Margarita Parada, Karina Tapia, Carlos Rubilar, Miguel L Allende, Oscar A Peña
Neutrophils are a major component of the innate immune response and the most abundant circulating cell type in humans and zebrafish. The CXCL12/CXCR4 ligand receptor pair plays a key role in neutrophil homeostasis, controlling definitive hematopoiesis and neutrophil release into circulation. Neutrophils overexpressing CXCR4 respond by migrating towards sources of CXCL12, which is abundant in hematopoietic tissues. However, the physiological role of CXCL12/CXCR4 signaling during inflammatory responses remains unknown...
February 20, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28215742/identification-of-grass-carp-ctenopharyngodon-idella-xbp1s-as-a-primary-member-in-er-stress
#20
Xiangqin Wang, Yichuan Mi, Bin Zhong, Huiling Mao, Yiqi Wan, Tao Zhang, Haizhou Wang, Chengyu Hu
X-box binding protein 1 (XBP1), a vital basic leucine zipper transcription factor for the related gene transcription in endoplasmic reticulum (ER) stress, belongs to the CREB/ATF family. In mammals, XBP1S is the activated one of XBP1 isoform. In order to study the role of fish XBP1S, we cloned and identified the XBP1S (KU509247) from grass carp (Ctenopharyngodon idella) (named CiXBP1S) by homologous cloning and RACE technique. The full length of CiXBP1S is 1694 bp along with 124 bp of 5' UTR, 418 bp of 3' UTR and the longest open reading frame (1152 bp) encoding a polypeptide of 383 amino acids with a well conserved DNA binding domain (BRLZ domain)...
May 2017: Fish & Shellfish Immunology
keyword
keyword
110573
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"