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https://www.readbyqxmd.com/read/29145131/removal-of-the-precursors-of-n-nitrosodiethylamine-ndea-an-emerging-disinfection-byproduct-in-drinking-water-treatment-process-and-its-toxicity-to-adult-zebrafish-danio-rerio
#1
Jian Zheng, Tao Lin, Wei Chen
N-nitrosodiethylamine (NDEA) is one of the emerging nitrogenous disinfection byproducts with probable cytotoxicity, genotoxicity, and carcinogenesis. Its potential toxicological effects have received extensive attention but remain to be poorly understood. In this study, changes in NDEA precursors in drinking water treatment process were studied using the trial of its formation potential (FP), and the toxicity induced by NDEA to adult zebrafish was investigated. NDEA FP in the raw water of Taihu Lake ranged from 46...
October 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/29142988/proximal-tubular-expression-patterns-of-megalin-and-cubilin-in-proteinuric-nephropathies
#2
Jia Sun, Kjell Hultenby, Jonas Axelsson, Johan Nordström, Bing He, Annika Wernerson, Karin Lindström
Introduction: Receptor-mediated endocytosis is responsible for protein reabsorption in the proximal tubules. For albumin this process involves at least 2 interacting receptors, megalin and cubilin. Albumin is not usually present in the urine, indicating a highly efficient tubular reuptake under physiological conditions. However, early appearance of albuminuria may mean that the tubular system is overwhelmed by large quantities of albumin or that the function is impaired. Methods: To better understand the physiological role of megalin and cubilin in human renal disease, renal biopsies from 15 patients with a range of albuminuria and 3 healthy living donors were analyzed for proximal tubular expression of megalin and cubilin using immunohistochemistry (IHC) and semiquantitative immune-electron microscopy...
July 2017: KI Reports
https://www.readbyqxmd.com/read/29111710/effect-of-pachybasin-on-general-toxicity-and-developmental-toxicity-in-vivo
#3
Yi-Ruu Lin, Ming-Huan Chan, Huan-Lin Peng, Kou-Cheng Peng, Shu-Ying Liu
In order to document the safety of pachybasin, a secondary metabolite of Trichoderma harzianum, for use as a bio-agricultural agent, it was subjected to general toxicological testing in mice and developmental toxicity in zebrafish. With either 5 or 20 mg kg-1 pachybasin i.p. injection, mice behavioral responses such as motor coordination, spontaneous locomotor activity, or nociceptive pain was not influenced. In long-term effect (daily injection for 14 days), the physiological, haematological, liver, and kidney functions were not altered either...
November 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29100090/exome-wide-association-study-identifies-greb1l-mutations-in-congenital-kidney-malformations
#4
Simone Sanna-Cherchi, Kamal Khan, Rik Westland, Priya Krithivasan, Lorraine Fievet, Hila Milo Rasouly, Iuliana Ionita-Laza, Valentina P Capone, David A Fasel, Krzysztof Kiryluk, Sitharthan Kamalakaran, Monica Bodria, Edgar A Otto, Matthew G Sampson, Christopher E Gillies, Virginia Vega-Warner, Katarina Vukojevic, Igor Pediaditakis, Gabriel S Makar, Adele Mitrotti, Miguel Verbitsky, Jeremiah Martino, Qingxue Liu, Young-Ji Na, Vinicio Goj, Gianluigi Ardissino, Maddalena Gigante, Loreto Gesualdo, Magdalena Janezcko, Marcin Zaniew, Cathy Lee Mendelsohn, Shirlee Shril, Friedhelm Hildebrandt, Joanna A E van Wijk, Adela Arapovic, Marijan Saraga, Landino Allegri, Claudia Izzi, Francesco Scolari, Velibor Tasic, Gian Marco Ghiggeri, Anna Latos-Bielenska, Anna-Materna Kiryluk, Shrikant Mane, David B Goldstein, Richard P Lifton, Nicholas Katsanis, Erica E Davis, Ali G Gharavi
Renal agenesis and hypodysplasia (RHD) are major causes of pediatric chronic kidney disease and are highly genetically heterogeneous. We conducted whole-exome sequencing in 202 case subjects with RHD and identified diagnostic mutations in genes known to be associated with RHD in 7/202 case subjects. In an additional affected individual with RHD and a congenital heart defect, we found a homozygous loss-of-function (LOF) variant in SLIT3, recapitulating phenotypes reported with Slit3 inactivation in the mouse...
November 2, 2017: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29093028/genome-wide-meta-analysis-unravels-interactions-between-magnesium-homeostasis-and-metabolic-phenotypes
#5
Tanguy Corre, Francisco J Arjona, Caroline Hayward, Sonia Youhanna, Jeroen H F de Baaij, Hendrica Belge, Nadine Nägele, Huguette Debaix, Maxime G Blanchard, Michela Traglia, Sarah E Harris, Sheila Ulivi, Rico Rueedi, David Lamparter, Aurélien Macé, Cinzia Sala, Stefania Lenarduzzi, Belen Ponte, Menno Pruijm, Daniel Ackermann, Georg Ehret, Daniela Baptista, Ozren Polasek, Igor Rudan, Toby W Hurd, Nicholas D Hastie, Veronique Vitart, Geràrd Waeber, Zoltán Kutalik, Sven Bergmann, Rosa Vargas-Poussou, Martin Konrad, Paolo Gasparini, Ian J Deary, John M Starr, Daniela Toniolo, Peter Vollenweider, Joost G J Hoenderop, René J M Bindels, Murielle Bochud, Olivier Devuyst
Magnesium (Mg(2+)) homeostasis is critical for metabolism. However, the genetic determinants of the renal handling of Mg(2+), which is crucial for Mg(2+) homeostasis, and the potential influence on metabolic traits in the general population are unknown. We obtained plasma and urine parameters from 9099 individuals from seven cohorts, and conducted a genome-wide meta-analysis of Mg(2+) homeostasis. We identified two loci associated with urinary magnesium (uMg), rs3824347 (P=4.4×10(-13)) near TRPM6, which encodes an epithelial Mg(2+) channel, and rs35929 (P=2...
November 1, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29091793/differential-sensitivities-to-dioxin-like-compounds-pcb-126-and-pecdf-between-tg-cyp1a-gfp-transgenic-medaka-and-zebrafish-larvae
#6
Hongyan Xu, Caixia Li, Pacharaporn Suklai, Qinghua Zeng, Raymond Chong, Zhiyuan Gong
It has been intensively documented that there are species-differences in the sensitivity to dioxin-like compounds (DLCs) in mammalian and avian. However, this issue is still unclear in fish. This study aimed at evaluating the differential sensitivities to DLCs in fish larvae. Here, larvae of Tg(cyp1a:gfp) medaka and Tg(cyp1a:gfp) zebrafish were tested with 2,3,7,8-Tetrachlorodibenzodioxin (TCDD), polychlorinated biphenyl 126 (PCB 126) and 2,3,4,7,8,-Pentachlorodibenzofuran (PeCDF). Comparative analyses were performed on induction of GFP fluorescence, expression of endogenous cyp1a mRNAs and EROD activity between the two species after exposure to these chemicals...
October 29, 2017: Chemosphere
https://www.readbyqxmd.com/read/29046299/high-content-screening-assay-based-discovery-of-paullones-as-novel-podocyte-protective-agents
#7
Ha Won Lee, Ehtesham Arif, Mehmet Mete Altintas, Kevin Quick, Shrey Maheshwari, Alexandra Plezia, Aqsa Mahmood, Jochen Reiser, Deepak Nihalani, Vineet Gupta
Podocyte dysfunction and loss is an early event and a hallmark of proteinuric kidney diseases. Podocyte's normal function is maintained via its unique cellular architecture that relies on an intracellular network of filaments, including filamentous actin (F-actin) and microtubules, that provides mechanical support. Damage to this filamentous network leads to changes in cellular morphology and results in podocyte injury, dysfunction, and death. Conversely, stabilization of this network protects podocytes and ameliorates proteinuria...
October 18, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28988229/studying-kidney-disease-using-tissue-and-genome-engineering-in-human-pluripotent-stem-cells
#8
Elena Garreta, Federico González, Núria Montserrat
Kidney morphogenesis and patterning have been extensively studied in animal models such as the mouse and zebrafish. These seminal studies have been key to define the molecular mechanisms underlying this complex multistep process. Based on this knowledge, the last 3 years have witnessed the development of a cohort of protocols allowing efficient differentiation of human pluripotent stem cells (hPSCs) towards defined kidney progenitor populations using two-dimensional (2D) culture systems or through generating organoids...
October 7, 2017: Nephron
https://www.readbyqxmd.com/read/28959735/zebrafish-danio-rerio-as-a-vertebrate-model-host-to-study-colonization-pathogenesis-and-transmission-of-foodborne-escherichia-coli-o157
#9
Daniel H Stones, Alexander G J Fehr, Laurel Thompson, Jacqueline Rocha, Nicolas Perez-Soto, Vipin T P Madhavan, Kerstin Voelz, Anne Marie Krachler
Foodborne infections with enterohemorrhagic Escherichia coli (EHEC) are a major cause of diarrheal illness in humans and can lead to severe complications such as hemolytic uremic syndrome. Cattle and other ruminants are the main reservoir of EHEC, which enters the food chain through contaminated meat, dairy, or vegetables. Here, we describe the establishment of a vertebrate model for foodborne EHEC infection, using larval zebrafish (Danio rerio) as a host and the protozoan prey Paramecium caudatum as a vehicle...
September 2017: MSphere
https://www.readbyqxmd.com/read/28947709/the-first-report-of-diablo-in-megalobrama-amblycephala-characterization-phylogenetic-analysis-functional-annotation-and-expression
#10
Ngoc Tuan Tran, Ivan Jakovlić, Wei-Min Wang
Smac/DIABLO gene is essential for the apoptosis mechanism in mammals. This study is the first report of the Megalobrama amblycephala (ma) diablo gene, and the first report of the tertiary structure of a Diablo polypeptide in fish. Madiablo is 1540-bp long with an open reading frame of 792 bp, encoding a putative protein of 263 amino acids with a molecular weight of 29.2 kDa. Phylogenetic analysis indicates that it is closely related to the zebrafish Diablo-a homologue. It also indicates the existence of two diablo copies (a and b) in teleosts; apart fromthe Percomorpha group,where diablo-b has been lost, but diablo-a had undergone an independent duplication...
September 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28944777/developmental-biology-single-cell-rna-sequencing-identifies-novel-kidney-cell-types-in-zebrafish
#11
Jack M Heintze
No abstract text is available yet for this article.
November 2017: Nature Reviews. Nephrology
https://www.readbyqxmd.com/read/28935902/targeting-neph1-and-zo-1-protein-protein-interaction-in-podocytes-prevents-podocyte-injury-and-preserves-glomerular-filtration-function
#12
Amin Sagar, Ehtesham Arif, Ashish Kumar Solanki, Pankaj Srivastava, Michael G Janech, Seok-Hyung Kim, Joshua H Lipschutz, Sang-Ho Kwon, Ashish, Deepak Nihalani
Targeting protein-protein interaction (PPI) is rapidly becoming an attractive alternative for drug development. While drug development commonly involves inhibiting a PPI, in this study, we show that stabilizing PPI may also be therapeutically beneficial. Junctional proteins Neph1 and ZO-1 and their interaction is an important determinant of the structural integrity of slit diaphragm, which is a critical component of kidney's filtration system. Since injury induces loss of this interaction, we hypothesized that strengthening this interaction may protect kidney's filtration barrier and preserve kidney function...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28932931/usefulness-of-zebrafish-larvae-to-evaluate-drug-induced-functional-and-morphological-renal-tubular-alterations
#13
Rita Gorgulho, Raquel Jacinto, Susana S Lopes, Sofia A Pereira, Erin M Tranfield, Gabriel G Martins, Emilio J Gualda, Rico J E Derks, Ana C Correia, Evelyne Steenvoorden, Petra Pintado, Oleg A Mayboroda, Emilia C Monteiro, Judit Morello
Prediction and management of drug-induced renal injury (DIRI) rely on the knowledge of the mechanisms of drug insult and on the availability of appropriate animal models to explore it. Zebrafish (Danio rerio) offers unique advantages for assessing DIRI because the larval pronephric kidney has a high homology with its human counterpart and it is fully mature at 3.5 days post-fertilization. Herein, we aimed to evaluate the usefulness of zebrafish larvae as a model of renal tubular toxicity through a comprehensive analysis of the renal alterations induced by the lethal concentrations for 10% of the larvae for gentamicin, paracetamol and tenofovir...
September 20, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28922741/genomic-and-biochemical-characterization-of-sialic-acid-acetylesterase-siae-in-zebrafish
#14
Viola Ravasio, Eufemia Damiati, Daniela Zizioli, Flavia Orizio, Edoardo Giacopuzzi, Marta Manzoni, Roberto Bresciani, Giuseppe Borsani, Eugenio Monti
Sialic acid acetylesterase (SIAE) removes acetyl moieties from the carbon 9 and 4 hydroxyl groups of sialic acid and recently a debate has been opened on its association to autoimmunity. Trying to get new insights on this intriguing enzyme we have studied siae in zebrafish (Danio rerio). In this teleost siae encodes for a polypeptide with a high degree of sequence identity to human and mouse counterparts. Zebrafish Siae behavior upon transient expression in COS7 cells is comparable to human enzyme concerning pH optimum of enzyme activity, subcellular localization and glycosylation...
October 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28921928/inverted-formins-a-subfamily-of-atypical-formins
#15
REVIEW
Anna Hegsted, Curtis V Yingling, David Pruyne
Formins are a family of regulators of actin and microtubule dynamics that are present in almost all eukaryotes. These proteins are involved in many cellular processes, including cytokinesis, stress fiber formation, and cell polarization. Here we review one subfamily of formins, the inverted formins. Inverted formins as a group break several formin stereotypes, having atypical biochemical properties and domain organization, and they have been linked to kidney disease and neuropathy in humans. In this review, we will explore recent research on members of the inverted formin sub-family in mammals, zebrafish, fruit flies, and worms...
September 16, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28910320/differential-gene-expression-following-tlr-stimulation-in-rag1-mutant-zebrafish-tissues-and-morphological-descriptions-of-lymphocyte-like-cell-populations
#16
Preeti J Muire, Larry A Hanson, Robert Wills, Lora Petrie-Hanson
In the absence of lymphocytes, rag1-/- mutant zebrafish develop protective immunity to bacteria. In mammals, induction of protection by innate immunity can be mediated by macrophages or natural killer (NK) cells. To elucidate potential responsive cell populations, we morphologically characterized lymphocyte-like cells (LLCs) from liver, spleen and kidney hematopoietic tissues. In fish, these cells include NK cells and Non-specific cytotoxic cells (NCCs). We also evaluated the transcriptional expression response of select genes that are important indicators of NK and macrophage activation after exposure to specific TLR ligands...
2017: PloS One
https://www.readbyqxmd.com/read/28890382/in%C3%A2-vitro-genotoxicity-and-in%C3%A2-vivo-subchronic-evaluation-of-the-anti-inflammatory-pyrazole-compound-lqfm021
#17
Soraia Santana de Moura, Renato Ivan de Ávila, Lara Barroso Brito, Rhaul de Oliveira, Gisele Augusto Rodrigues de Oliveira, Francine Pazini, Ricardo Menegatti, Aline Carvalho Batista, Cesar Koppe Grisolia, Marize Campos Valadares
Scientific evidences have highlighted 5-(1-(3-fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazole (LQFM021) as a promising anti-inflammatory, analgesic and antinociceptive agent due to its effects on peripheral opioid receptors associated with activation of the nitric oxide/cGMP/KATP pathway. Despite these important pharmacological findings, toxicity data of LQFM021 are scarce. Thus, this study investigated the in vitro genotoxicity of LQFM021 through cytokinesis-block micronucleus assay (OECD Nº 487/2014). Moreover, zebrafish model was used to assess the embryotoxicity potential of LQFM021 using fish embryo toxicity test (OECD Nº 236/2013) with extended exposure to evaluate subchronic larval development...
November 1, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28878000/dissecting-hematopoietic-and-renal-cell-heterogeneity-in-adult-zebrafish-at-single-cell-resolution-using-rna-sequencing
#18
Qin Tang, Sowmya Iyer, Riadh Lobbardi, John C Moore, Huidong Chen, Caleb Lareau, Christine Hebert, McKenzie L Shaw, Cyril Neftel, Mario L Suva, Craig J Ceol, Andre Bernards, Martin Aryee, Luca Pinello, Iain A Drummond, David M Langenau
Recent advances in single-cell, transcriptomic profiling have provided unprecedented access to investigate cell heterogeneity during tissue and organ development. In this study, we used massively parallel, single-cell RNA sequencing to define cell heterogeneity within the zebrafish kidney marrow, constructing a comprehensive molecular atlas of definitive hematopoiesis and functionally distinct renal cells found in adult zebrafish. Because our method analyzed blood and kidney cells in an unbiased manner, our approach was useful in characterizing immune-cell deficiencies within DNA-protein kinase catalytic subunit (prkdc), interleukin-2 receptor γ a (il2rga), and double-homozygous-mutant fish, identifying blood cell losses in T, B, and natural killer cells within specific genetic mutants...
October 2, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28860076/the-antiviral-role-of-heat-shock-protein-27-against-red-spotted-grouper-nervous-necrosis-virus-infection-in-sea-perch
#19
Yao Le, Peng Jia, Yilin Jin, Wei Liu, Kuntong Jia, Meisheng Yi
Heat shock protein 27 (HSP27), functioning as a stress induced protective protein, has been reported to participate in various biological processes, including apoptosis, thermal protection, and virus infection. In this study, a HSP27-like gene from the seawater fish sea perch, designated as LjHSP27, was characterized. The 1361 bp full-length cDNA of LjHSP27 encoded a 221 amino acid protein containing a conserved α-crystallin domain, two variable amino- and carboxy-terminal extensions, a WD/EPF motif, two serine phosphorylation sites, and two putative actin binding regions...
August 30, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28852872/amino-acids-are-major-energy-substrates-for-tissues-of-hybrid-striped-bass-and-zebrafish
#20
Sichao Jia, Xinyu Li, Shixuan Zheng, Guoyao Wu
Fish generally have much higher requirements for dietary protein than mammals, and this long-standing puzzle remains unsolved. The present study was conducted with zebrafish (omnivores) and hybrid striped bass (HSB, carnivores) to test the hypothesis that AAs are oxidized at a higher rate than carbohydrates (e.g., glucose) and fatty acids (e.g., palmitate) to provide ATP for their tissues. Liver, proximal intestine, kidney, and skeletal muscle isolated from zebrafish and HSB were incubated at 28.5 °C (zebrafish) or 26 °C (HSB) for 2 h in oxygenated Krebs-Henseleit bicarbonate buffer (pH 7...
August 29, 2017: Amino Acids
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