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https://www.readbyqxmd.com/read/29720549/the-role-of-palladin-in-podocytes
#1
Nadine Artelt, Tim A Ludwig, Henrik Rogge, Panagiotis Kavvadas, Florian Siegerist, Antje Blumenthal, Jens van den Brandt, Carol A Otey, Marie-Louise Bang, Kerstin Amann, Christos E Chadjichristos, Christos Chatziantoniou, Karlhans Endlich, Nicole Endlich
Background Podocyte loss and effacement of interdigitating podocyte foot processes are the major cause of a leaky filtration barrier and ESRD. Because the complex three-dimensional morphology of podocytes depends on the actin cytoskeleton, we studied the role in podocytes of the actin bundling protein palladin, which is highly expressed therein. Methods We knocked down palladin in cultured podocytes by siRNA transfection or in zebrafish embryos by morpholino injection and studied the effects by immunofluorescence and live imaging...
May 2, 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29707605/scaling-up-to-study-brca2-the-zeppelin-zebrafish-mutant-reveals-a-role-for-brca2-in-embryonic-development-of-kidney-mesoderm
#2
Bridgette E Drummond, Rebecca A Wingert
Specialized renal epithelial cells known as podocytes are essential components of the filtering structures within the kidney that coordinate the process of removing waste from the bloodstream. Podocyte loss initiates many human kidney diseases as it triggers subsequent damage to the kidney, leading to progressive loss of function that culminates with end stage renal failure. Podocyte morphology, function and gene expression profiles are well conserved between zebrafish and humans, making the former a relevant model to study podocyte development and model kidney diseases...
2018: Cancer Cell & Microenvironment
https://www.readbyqxmd.com/read/29689295/behavioral-and-biochemical-effects-of-ethanol-withdrawal-in-zebrafish
#3
Suianny Nayara da Silva Chaves, Gabriel Felício Rocha, Bruna Patrícia Dutra Costa, Witallo Etevaldo Araújo de Oliveira, Monica Gomes Lima, Diógenes Henrique de Siqueira Silva, Caio Maximino
Chronic alcohol use induces adaptations and toxicity that can induce symptoms of anxiety, autonomic hyperarousal, and epileptic seizures when alcohol is removed (withdrawal syndrome). Zebrafish has recently gained wide attention as a behavioral model to study the neurobehavioral effects of acute and chronic alcohol use, including withdrawal. The literature, however, is very contradictory on findings regarding withdrawal effects, with some studies reporting increased anxiety, while others report no effect. A meta-analytic approach was taken to find the sources of this heterogeneity, and ethanol concentration during exposure and exposure duration were found to be the main sources of variation...
April 21, 2018: Pharmacology, Biochemistry, and Behavior
https://www.readbyqxmd.com/read/29684692/acute-toxic-responses-of-embryo-larval-zebrafish-to-zinc-pyrithione-zpt-reveal-embryological-and-developmental-toxicity
#4
Ye Zhao, Yuyang Liu, Jing Sun, Hongtao Sha, Yu Yang, Qian Ye, Qi Yang, Baoqi Huang, Yadong Yu, He Huang
Zinc pyrithione (ZPT) is widely used in industrial and human daily life, due to its broad antimicrobial spectrum activity. Persistent accumulation of ZTP in the aquatic environment and bioaccumulation in the living organisms attracts more and more attention. However, only very limited information is available so far for the evaluation of systematic toxicity effects of ZPT on multiple organs development. This study intends to deepen our knowledge about the potential toxicity elicited by ZPT by assessing its acute effects on zebrafish (Danio rerio) through morphological, histological and molecular investigations...
April 6, 2018: Chemosphere
https://www.readbyqxmd.com/read/29590089/whole-organism-clone-tracing-using-single-cell-sequencing
#5
Anna Alemany, Maria Florescu, Chloé S Baron, Josi Peterson-Maduro, Alexander van Oudenaarden
Embryonic development is a crucial period in the life of a multicellular organism, during which limited sets of embryonic progenitors produce all cells in the adult body. Determining which fate these progenitors acquire in adult tissues requires the simultaneous measurement of clonal history and cell identity at single-cell resolution, which has been a major challenge. Clonal history has traditionally been investigated by microscopically tracking cells during development, monitoring the heritable expression of genetically encoded fluorescent proteins and, more recently, using next-generation sequencing technologies that exploit somatic mutations, microsatellite instability, transposon tagging, viral barcoding, CRISPR-Cas9 genome editing and Cre-loxP recombination...
April 5, 2018: Nature
https://www.readbyqxmd.com/read/29588376/trrap-is-a-central-regulator-of-human-multiciliated-cell-formation
#6
Zhao Wang, Lindsey W Plasschaert, Shivani Aryal, Nicole A Renaud, Zinger Yang, Rayman Choo-Wing, Angelica D Pessotti, Nathaniel D Kirkpatrick, Nadire R Cochran, Walter Carbone, Rob Maher, Alicia Lindeman, Carsten Russ, John Reece-Hoyes, Gregory McAllister, Gregory R Hoffman, Guglielmo Roma, Aron B Jaffe
The multiciliated cell (MCC) is an evolutionarily conserved cell type, which in vertebrates functions to promote directional fluid flow across epithelial tissues. In the conducting airway, MCCs are generated by basal stem/progenitor cells and act in concert with secretory cells to perform mucociliary clearance to expel pathogens from the lung. Studies in multiple systems, including Xenopus laevis epidermis, murine trachea, and zebrafish kidney, have uncovered a transcriptional network that regulates multiple steps of multiciliogenesis, ultimately leading to an MCC with hundreds of motile cilia extended from their apical surface, which beat in a coordinated fashion...
March 27, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29560575/molecular-characterization-and-identification-of-facilitative-glucose-transporter-2-glut2-and-its-expression-and-of-the-related-glycometabolism-enzymes-in-response-to-different-starch-levels-in-blunt-snout-bream-megalobrama-amblycephala
#7
Hualiang Liang, Ahmed Mokrani, Hopeson Chisomo-Kasiya, Ogwok-Manas Wilson-Arop, Haifeng Mi, Ke Ji, Xianping Ge, Mingchun Ren
Facilitative glucose transporters (GLUT) are transmembrane transporters involved in glucose transport across the plasma membrane. In this study, blunt snout bream GLUT2 gene was cloned, and its expression in various tissues and in liver in response to diets with different carbohydrate levels (17.1; 21.8; 26.4; 32.0; 36.3; and 41.9% of dry matter). Blunt snout bream GLUT2 was also characterized. A full-length cDNA fragment of 2577 bp was cloned, which contains a 5'-untranslated region (UTR) of 73 bp, a 3'-UTR of 992 bp, and an open reading frame of 1512 bp that encodes a polypeptide of 503 amino acids with predicted molecular mass of 55...
March 20, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29553143/zebrafish-as-models-to-study-ciliopathies-of-the-eye-and-kidney
#8
Yi Shi, Yanhui Su, Joshua H Lipschutz, Glenn P Lobo
Cilia are highly-conserved organelles projecting from the cell surface of nearly every cell type in vertebrates. Ciliary proteins have essential functions in human physiology, particularly in signaling and organ development. As cilia are a component of almost all vertebrate cells, cilia dysfunction can manifest as a constellation of features that characteristically include, retinal degeneration, renal disease and cerebral anomalies. The terminology "Ciliopathies" refers to inherited human disorders caused by genetic mutations in ciliary genes, leading to cilia dysfunctions that form an important and ever expanding multi-organ disease spectrum...
December 2017: Clinical nephrology and research
https://www.readbyqxmd.com/read/29537312/a-zebrafish-model-of-infection-associated-acute-kidney-injury
#9
Xiaoyan Wen, Liyan Cui, Seth Morrisroe, Donald Maberry, David R Emlet, Simon C Watkins, Neil A Hukriede, John A Kellum
Sepsis-associated acute kidney injury (S-AKI) independently predicts mortality among critically ill patients. The role of innate immunity in this process is unclear, and there is an unmet need for S-AKI models to delineate the pathophysiological response. Mammals and zebrafish (Danio rerio) share a conserved nephron structure and homologous innate immune systems, making the latter suitable for sepsis-associated AKI research. We introduced Edwardsiella tarda (E.tarda) to the zebrafish. Systemic E. tarda bacteremia resulted in sustained bacterial infection and dose-dependent mortality...
March 14, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29529391/scribble-influences-cyst-formation-in-autosomal-dominant-polycystic-kidney-disease-by-regulating-hippo-signaling-pathway
#10
Dechao Xu, Jiayi Lv, Liangliang He, Lili Fu, Ruikun Hu, Ying Cao, Changlin Mei
Polarity complexes, including the PAR (Partitioning-defective), CRB (Crumbs) and SCRIB (Scribble) complexes, are required for the physiologic establishment, stabilization, and maintenance of a functional apical-basolateral polarity. Inactivation of some of the polarity complexes results in cystic kidneys, and apical-basolateral polarity defects are commonly observed in autosomal-dominant polycystic kidney disease (ADPKD); however, little is known about the role that polarity complexes play in ADPKD. Here, we demonstrate that Scribble, a core protein of the SCRIB complex, is down-regulated in ADPKD cell lines and the zebrafish model of this disease ( pkd2 morphants)...
March 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29502161/zebrafish-as-a-model-for-kidney-function-and-disease
#11
REVIEW
Priya Outtandy, Claire Russell, Robert Kleta, Detlef Bockenhauer
Kidney disease is a global problem with around three million people diagnosed in the UK alone and the incidence is rising. Research is critical to develop better treatments. Animal models can help to better understand the pathophysiology behind the various kidney diseases and to screen for therapeutic compounds, but the use especially of mammalian models should be minimised in the interest of animal welfare. Zebrafish are increasingly used, as they are genetically tractable and have a basic renal anatomy comparable to mammalian kidneys with glomerular filtration and tubular filtration processing...
March 3, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/29498212/the-transcription-factor-dach1-is-essential-for-podocyte-function
#12
Nicole Endlich, Felix Kliewe, Frances Kindt, Katharina Schmidt, Ahmed M Kotb, Nadine Artelt, Maja T Lindenmeyer, Clemens D Cohen, Franziska Döring, Andreas W Kuss, Kerstin Amann, Marcus J Moeller, Nazanin Kabgani, Antje Blumenthal, Karlhans Endlich
Dedifferentiation and loss of podocytes are the major cause of chronic kidney disease. Dach1, a transcription factor that is essential for cell fate, was found in genome-wide association studies to be associated with the glomerular filtration rate. We found that podocytes express high levels of Dach1 in vivo and to a much lower extent in vitro. Parietal epithelial cells (PECs) that are still under debate to be a type of progenitor cell for podocytes expressed Dach1 only at low levels. The transfection of PECs with a plasmid encoding for Dach1 induced the expression of synaptopodin, a podocyte-specific protein, demonstrated by immunocytochemistry and Western blot...
May 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29483630/first-profiling-of-lysine-crotonylation-of-myofilament-proteins-and-ribosomal-proteins-in-zebrafish-embryos
#13
Oh Kwang Kwon, Sun Joo Kim, Sangkyu Lee
Zebrafish embryos are translucent and develop rapidly in individual eggs ex utero; they are widely used as models for embryogenesis and organ development for human diseases and drug discovery. Lysine crotonylation (Kcr) is a type of histone post-translational modifications discovered in 2011. Kcr dynamics are involved in gene expression regulation and acute kidney injury; however, little is known about the effects of Kcr on non-histone proteins. In the present study, we conducted the first proteome-wide profiling of Kcr in zebrafish larvae and identified 557 Kcr sites on 218 proteins, representing the Kcr event in zebrafish...
February 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29480793/the-efficacy-of-puromycin-and-adriamycin-for-induction-of-glomerular-failure-in-larval-zebrafish-validated-by-an-assay-of-glomerular-permeability-dynamics
#14
Sebastien Andrew Rider, Finnius Austin Bruton, Richard George Collins, Bryan Ronald Conway, John James Mullins
Defects in the glomerular filtration barrier (GFB) play a major role in the onset of human renal diseases. Highly ramified glomerular cells named podocytes are a critical component of the GFB. Injury to podocytes results in abnormal excretion of plasma proteins, which can lead to chronic kidney disease. The conserved paired nephron of larval zebrafish is an excellent model for assessing glomerular function and injury. The efficacy of two known podocyte toxins was tested to refine models of acute podocyte injury in larval zebrafish...
February 26, 2018: Zebrafish
https://www.readbyqxmd.com/read/29472535/egf-receptor-kinase-suppresses-ciliogenesis-through-activation-of-usp8-deubiquitinase
#15
Kousuke Kasahara, Hiromasa Aoki, Tohru Kiyono, Shujie Wang, Harumi Kagiwada, Mizuki Yuge, Toshio Tanaka, Yuhei Nishimura, Akira Mizoguchi, Naoki Goshima, Masaki Inagaki
Ciliogenesis is generally inhibited in dividing cells, however, it has been unclear which signaling cascades regulate the phenomenon. Here, we report that epidermal growth factor receptor (EGFR) kinase suppresses ciliogenesis by directly phosphorylating the deubiquitinase USP8 on Tyr-717 and Tyr-810 in RPE1 cells. These phosphorylations elevate the deubiquitinase activity, which then stabilizes the trichoplein-Aurora A pathway, an inhibitory mechanism of ciliogenesis. EGFR knockdown and serum starvation result in ciliogenesis through downregulation of the USP8-trichoplein-Aurora A signal...
February 22, 2018: Nature Communications
https://www.readbyqxmd.com/read/29425915/toxicokinetic-and-toxicodynamic-of-depleted-uranium-in-the-zebrafish-danio-rerio
#16
Olivier Simon, Béatrice Gagnaire, Virginie Camilleri, Isabelle Cavalié, Magali Floriani, Christelle Adam-Guillermin
This study investigated the accumulation pattern and biological effects (genotoxicity and histopathology) to adult zebrafish (male and female) exposed to a nominal waterborne concentration of 20 μg L-1 of depleted uranium (DU) for 28 days followed by 27 days of depuration. Accumulation pattern showed that (i) DU accumulated in brain, (ii) levels in digestive tract were higher than those measured in gills and (iii) levels remained high in kidney, brain and ovary despite the 27 days of depuration period...
April 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29422865/dietary-safety-assessment-of-flk1-transgenic-fish
#17
Yalan Wei, Ling Huang, Jinghui Cao, Chenghui Wang, Jizhou Yan
Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding Flk1-transgenic larvae model to examine the predatory fish's histology in multiple tissues, and the global gene expression profile in the liver...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29408220/a-potent-tilapia-secreted-granulin-peptide-enhances-the-survival-of-transgenic-zebrafish-infected-by-vibrio-vulnificus-via-modulation-of-innate-immunity
#18
Sheng-Han Wu, Hong-Jie Lin, Wen-Fu Lin, Jen-Leih Wu, Hong-Yi Gong
Progranulin (PGRN) is a multi-functional growth factor that mediates cell proliferation, survival, migration, tumorigenesis, wound healing, development, and anti-inflammation activity. A novel alternatively spliced transcript from the short-form PGRN1 gene encoding a novel, secreted GRN peptide composed of 20-a.a. signal peptide and 41-a.a. GRN named GRN-41 was identified to be abundantly expressed in immune-related organs including spleen, head kidney, and intestine of Mozambique tilapia. The expression of GRN-41 and PGRN1 were further induced in the spleen of tilapia challenged with Vibrio vulnificus at 3 h post infection (hpi) and 6 hpi, respectively...
April 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29341136/phylogeny-and-distribution-of-protein-kinase-c-variants-in-the-zebrafish
#19
Marion F Haug, Matthias Gesemann, Manuela Berger, Stephan C F Neuhauss
Conventional protein kinases - consisting of α, β, and γ family members - play key roles in numerous signal transduction events. Phylogenetic analysis demonstrated the existence of five prkcs (the genes representing PKCs) in zebrafish, two paralogous forms of prkca and -b and one prkcg variant. mRNA expression analysis showed distinct, mainly nervous system specific expression, for all five prkc genes. For prkca and prkcb paralogs prominent expression can be seen in the telencephalon, in diencephalic regions such as the habenula or the optic tectum, in hypothalamic areas and in distinct cerebellar structures...
January 17, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29317240/toxicity-and-cardiac-effects-of-acute-exposure-to-tryptophan-metabolites-on-the-kynurenine-pathway-in-early-developing-zebrafish-danio-rerio-embryos
#20
Michal Majewski, Natalia Kasica, Anna Jakimiuk, Piotr Podlasz
Defects in tryptophan metabolism on the l-kynurenine pathway (KP) are implicated in a number of human diseases, including chronic kidney disease, brain edema or injury, tuberculosis and malaria - as well as cancer, neurodegenerative and autoimmune disorders. However, it is unclear to what extent detrimental effects of exposure to tryptophan metabolites might impact the early development of organism. Thus, this study examined the effects of KP exposure in zebrafish embryos starting at the blastula period (4hpf) and the segmentation stage (24hpf)...
February 15, 2018: Toxicology and Applied Pharmacology
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