Ronan Keegan, David G Waterman, David J Hopper, Leighton Coates, Graham Taylor, Jingxu Guo, Alun R Coker, Peter T Erskine, Steve P Wood, Jonathan B Cooper
During efforts to crystallize the enzyme 2,4-dihydroxyacetophenone dioxygenase (DAD) from Alcaligenes sp. 4HAP, a small number of strongly diffracting protein crystals were obtained after two years of crystal growth in one condition. The crystals diffracted synchrotron radiation to almost 1.0 Å resolution and were, until recently, assumed to be formed by the DAD protein. However, when another crystal form of this enzyme was eventually solved at lower resolution, molecular replacement using this new structure as the search model did not give a convincing solution with the original atomic resolution data set...
August 2016: Acta Crystallographica. Section D, Structural Biology