keyword
MENU ▼
Read by QxMD icon Read
search

4HAP

keyword
https://www.readbyqxmd.com/read/27487824/the-1-1%C3%A2-%C3%A3-resolution-structure-of-a-periplasmic-phosphate-binding-protein-from-stenotrophomonas-maltophilia-a-crystallization-contaminant-identified-by-molecular-replacement-using-the-entire-protein-data-bank
#1
Ronan Keegan, David G Waterman, David J Hopper, Leighton Coates, Graham Taylor, Jingxu Guo, Alun R Coker, Peter T Erskine, Steve P Wood, Jonathan B Cooper
During efforts to crystallize the enzyme 2,4-dihydroxyacetophenone dioxygenase (DAD) from Alcaligenes sp. 4HAP, a small number of strongly diffracting protein crystals were obtained after two years of crystal growth in one condition. The crystals diffracted synchrotron radiation to almost 1.0 Å resolution and were, until recently, assumed to be formed by the DAD protein. However, when another crystal form of this enzyme was eventually solved at lower resolution, molecular replacement using this new structure as the search model did not give a convincing solution with the original atomic resolution data set...
August 2016: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/27283534/monitoring-the-biodegradability-of-bisphenol-a-and-its-metabolic-intermediates-by-manometric-respirometry-tests
#2
A M Ferro Orozco, E M Contreras, N E Zaritzky
As a result of its wide usage in the production of polycarbonate plastics and epoxy resins, bisphenol A (BPA) is commonly detected in wastewaters. Recently, BPA became a major concern because its adverse effects as an endocrine disruptor. In this work, the biodegradation kinetics of BPA and its metabolic intermediates 4-hydroxyacetophenone (4HAP), 4-hydroxybenzaldehyde (4HB) and 4-hydroxybenzoic acid (4HBA) by BPA-acclimated activated sludge was studied using manometric respirometry (BOD) tests. For all the tested compounds, BOD curves exhibited two phases...
June 9, 2016: Biodegradation
https://www.readbyqxmd.com/read/27171920/sem-and-tem-for-structure-and-properties-characterization-of-bacterial-cellulose-hydroxyapatite-composites
#3
Natalia A Arkharova, Elena I Suvorova, Alexander V Severin, Albert K Khripunov, Sergey V Krasheninnikov, Vera V Klechkovskaya
Preparation of composites with different properties and gradient of components is aimed at better performance of materials for bone substitution. Bacterial cellulose-hydroxyapatite (BC-HAP) composites with various mass ratio of the components (BC-25HAP, BC-4HAP, and BC-HAP) were prepared by a novel method of growing HAP nanocrystals (the linear size ≤30 nm) in water solutions in the presence of the BC gel-film micro-fragments. Varying the BC-HAP ratios leads to a gradual change of the physical properties of the materials...
May 12, 2016: Scanning
https://www.readbyqxmd.com/read/26457516/extension-of-resolution-and-oligomerization-state-studies-of-2-4-dihydroxyacetophenone-dioxygenase-from-alcaligenes-sp-4hap
#4
J Guo, P Erskine, A R Coker, J Gor, S J Perkins, S P Wood, J B Cooper
The enzyme 2,4'-dihydroxyacetophenone dioxygenase (DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid. This enzyme is a very unusual dioxygenase in that it cleaves a C-C bond in a substituent of the aromatic ring rather than within the ring itself. Whilst it has been shown that DAD is a tetramer in solution, the recently solved crystal structure of the Alcaligenes sp. 4HAP enzyme was in fact dimeric rather than tetrameric. Since the use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, was necessary for crystallization of the protein, it was investigated whether this was responsible for the change in its oligomerization state...
October 2015: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/25528503/conformational-stability-spectroscopic-ft-ir-ft-raman-and-uv-vis-analysis-nlo-nbo-fmo-and-fukui-function-analysis-of-4-hexylacetophenone-by-density-functional-theory
#5
S Saravanan, V Balachandran
The experimental and theoretical study on the structures and vibrations of 4-hexylacetophenone (abbreviated as 4HAP) are presented. The FT-IR and FT-Raman spectra of the title compound have been recorded in the region 4000-400cm(-1) and 3500-100cm(-1) respectively. The molecular structures, vibrational wavenumbers, infrared intensities and Raman activities were calculated using DFT (B3LYP and LSDA) method with 6-311++G(d,p) basis set. The most stable conformer of 4HAP is identified from the computational results...
March 5, 2015: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/25195757/structure-of-the-2-4-dihydroxyacetophenone-dioxygenase-from-alcaligenes-sp-4hap
#6
R Keegan, A Lebedev, P Erskine, J Guo, S P Wood, D J Hopper, S E J Rigby, J B Cooper
The enzyme 2,4'-dihydroxyacetophenone dioxygenase (DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. Whilst the vast majority of dioxygenases cleave within the aromatic ring of the substrate, DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring. There is evidence that the enzyme is a homotetramer of 20.3 kDa subunits, each containing nonhaem iron, and its sequence suggests that it belongs to the cupin family of dioxygenases...
September 2014: Acta Crystallographica. Section D, Biological Crystallography
https://www.readbyqxmd.com/read/24915102/crystallization-and-preliminary-x-ray-characterization-of-the-2-4-dihydroxyacetophenone-dioxygenase-from-alcaligenes-sp-4hap
#7
G Beaven, A Bowyer, P Erskine, S P Wood, A McCoy, L Coates, R Keegan, A Lebedev, D J Hopper, M A Kaderbhai, J B Cooper
The enzyme 2,4'-dihydroxyacetophenone dioxygenase (or DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. Whilst the vast majority of dioxygenases cleave within the aromatic ring of the substrate, DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring. There is evidence that the enzyme is a homotetramer of 20.3 kDa subunits each containing nonhaem iron and its sequence suggests that it belongs to the cupin family of dioxygenases...
June 2014: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/10567221/2-4-dihydroxyacetophenone-dioxygenase-ec-1-13-11-41-from-alcaligenes-sp-4hap-a-novel-enzyme-with-an-atypical-dioxygenase-sequence
#8
D J Hopper, M A Kaderbhai
2,4'-Dihydroxyacetophenone dioxygenase (EC 1.13.11.41) was purified to homogeneity from Alcaligenes sp. 4HAP grown on 4-hydroxyacetophenone. Measurements of the M(r) of the native enzyme ranged from 81600 to 87000, whereas values of 21000 and 20379 were given by SDS/PAGE and electrospray MS respectively. The enzyme is a homotetramer and contains one atom of iron per molecule of enzyme. From C- and N-terminal analyses, primers for PCR were designed and the dad gene cloned and sequenced. The predicted amino acid sequence of dad, deduced from the nucleotide sequence, confirms the N-terminal amino acid sequencing data and contains the sequence of an internal tryptic peptide...
December 1, 1999: Biochemical Journal
1
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"