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https://www.readbyqxmd.com/read/28513721/programmable-micrometer-sized-motor-array-based-on-live-cells
#1
Shuangxi Xie, Xiaodong Wang, Niandong Jiao, Steve Tung, Lianqing Liu
Trapping and transporting microorganisms with intrinsic motility are important tasks for biological, physical, and biomedical applications. However, fast swimming speed makes the manipulation of these organisms an inherently challenging task. In this study, we demonstrated that an optoelectrical technique, namely, optically induced dielectrophoresis (ODEP), could effectively trap and manipulate Chlamydomonas reinhardtii (C. reinhardtii) cells swimming at velocities faster than 100 μm s(-1). Furthermore, live C...
June 13, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28115307/fast-isolation-and-ex-vivo-culture-of-circulating-tumor-cells-from-the-peripheral-blood-of-lung-cancer-patients
#2
Wu Wenjun, Wang Zhihua, Wang Zhuo, Deng Yuliang, Shi Qihui
Circulating tumor cells (CTCs) are free tumor cells shed from tumor site and enter into blood circulation. CTCs represent a reliable source of tumor cells for the molecular characteristics of the original tumor. However, the extraordinary rarity of CTCs makes the subsequent molecular and functional analysis technically challenging. Here, we describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability (>90%) of CTCs during the in vitro isolation...
January 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/27995235/liquid-capped-encoded-microcapsules-for-multiplex-assays
#3
Younghoon Song, Yunjin Jeong, Taehong Kwon, Daewon Lee, Dong Yoon Oh, Tae-Joon Park, Junhoi Kim, Jiyun Kim, Sunghoon Kwon
Although droplet microfludics is a promising technology for handling a number of liquids of a single type of analyte, it has limitations in handling thousands of different types of analytes for multiplex assay. Here, we present a novel "liquid-capped encoded microcapsule", which is applicable to various liquid format assays. Various liquid drops can be graphically encoded and arrayed without repeated dispensing processes, evaporation, and the risk of cross-contamination. Millions of nanoliter-scale liquids are encapsulated within encoded microcapsules and self-assembled in microwells in a single dispensing process...
December 20, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27750100/convective-exosome-tracing-microfluidics-for-analysis-of-cell-non-autonomous-neurogenesis
#4
Hyun Jeong Oh, Yoojin Shin, Seok Chung, Do Won Hwang, Dong Soo Lee
The effective role of exosome delivering neurogenic microRNA (miRNA) enables to induce efficient differentiation process during neurogenesis. The microfludic system capable of visualizing the exosomal behavior such as secretion, migration, and uptake of individual exosomes can be used as a robust technique to understand the exosome-mediated change of cellular behavior. Here, we developed the exosome-tracing microfluidic system to visualize exosomal transport carrying the neurogenic miRNA from leading to neighboring cells, and found a new mode of exosome-mediated cell-non-autonomous neurogenesis...
October 5, 2016: Biomaterials
https://www.readbyqxmd.com/read/27087832/imaging-nanophotonic-modes-of-microresonators-using-a-focused-ion-beam
#5
Kevin A Twedt, Jie Zou, Marcelo Davanco, Kartik Srinivasan, Jabez J McClelland, Vladimir A Aksyuk
Optical microresonators have proven powerful in a wide range of applications, including cavity quantum electrodynamics(1-3), biosensing(4), microfludics(5), and cavity optomechanics(6-8). Their performance depends critically on the exact distribution of optical energy, confined and shaped by the nanoscale device geometry. Near-field optical probes(9) can image this distribution, but the physical probe necessarily perturbs the near field, which is particularly problematic for sensitive high quality factor resonances(10,11)...
2016: Nature Photonics
https://www.readbyqxmd.com/read/26887792/ex-vivo-expansion-of-circulating-lung-tumor-cells-based-on-one-step-microfluidics-based-immunomagnetic-isolation
#6
Zhihua Wang, Wenjun Wu, Zhuo Wang, Ying Tang, Yuliang Deng, Ling Xu, Jianhui Tian, Qihui Shi
We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs...
June 7, 2016: Analyst
https://www.readbyqxmd.com/read/26753780/geometric-effects-in-microfluidics-on-heterogeneous-cell-stress-using-an-eulerian-lagrangian-approach
#7
K M Warren, J N Mpagazehe, P R LeDuc, C F Higgs
The response of individual cells at the micro-scale in cell mechanics is important in understanding how they are affected by changing environments. To control cell stresses, microfluidics can be implemented since there is tremendous control over the geometry of the devices. Designing microfluidic devices to induce and manipulate stress levels on biological cells can be aided by computational modeling approaches. Such approaches serve as an efficient precursor to fabricating various microfluidic geometries that induce predictable levels of stress on biological cells, based on their mechanical properties...
February 7, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/26709385/scaffold-integrated-microchips-for-end-to-end-in-vitro-tumor-cell-attachment-and-xenograft-formation
#8
Jungwoo Lee, Nathaniel Kohl, Sachin Shanbhang, Biju Parekkadan
Microfluidic technologies have substantially advanced cancer research by enabling the isolation of rare circulating tumor cells (CTCs) for diagnostic and prognostic purposes. The characterization of isolated CTCs has been limited due to the difficulty in recovering and growing isolated cells with high fidelity. Here, we present a strategy that uses a 3D scaffold, integrated into a microfludic device, as a transferable substrate that can be readily isolated after device operation for serial use in vivo as a transplanted tissue bed...
December 2015: Technology
https://www.readbyqxmd.com/read/26365725/-applicability-of-new-diagnostic-techniques-in-microbiology-technological-innovation
#9
REVIEW
Rafael Cantón, Elena Loza, José Romero
Different new techniques have been introduced in microbiology laboratories during the last years, including mass spectrometry and next generation sequencing. These techniques, in addition to automation, microfludics, nanotechnology and informatics, have impelled innovation in the prevention and management of patients with infectious diseases. These approaches are relevant for revitalization and consolidation Clinical Microbiology laboratories.
September 2015: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/25763691/calcium-powered-phloem-protein-of-seo-gene-family-forisome-functions-in-wound-sealing-and-act-as-biomimetic-smart-materials
#10
REVIEW
Vineet Kumar Srivastava, Narendra Tuteja
Forisomes protein belongs to SEO gene family and is unique to Fabaceae family. These proteins are located in sieve tubes of phloem and function to prevent loss of nutrient-rich photoassimilates, upon mechanical injury/wounding. Forisome protein is also known as ATP independent, mechanically active proteins. Despite the wealth of information role of forisome in plants are not yet fully understood. Recent reports suggest that forisomes protein can act as ideal model to study self assembly mechanism for development of nanotechnological devices like microfluidic system application in space exploration mission...
2014: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/25732960/egfr-targeted-theranostic-nanoemulsion-for-image-guided-ovarian-cancer-therapy
#11
Srinivas Ganta, Amit Singh, Praveen Kulkarni, Amanda W Keeler, Aleksandr Piroyan, Rupa R Sawant, Niravkumar R Patel, Barbara Davis, Craig Ferris, Sara O'Neal, William Zamboni, Mansoor M Amiji, Timothy P Coleman
PURPOSE: Platinum-based therapies are the first line treatments for most types of cancer including ovarian cancer. However, their use is associated with dose-limiting toxicities and resistance. We report initial translational studies of a theranostic nanoemulsion loaded with a cisplatin derivative, myrisplatin and pro-apoptotic agent, C6-ceramide. METHODS: The surface of the nanoemulsion is annotated with an endothelial growth factor receptor (EGFR) binding peptide to improve targeting ability and gadolinium to provide diagnostic capability for image-guided therapy of EGFR overexpressing ovarian cancers...
August 2015: Pharmaceutical Research
https://www.readbyqxmd.com/read/24905822/calcium-powered-phloem-protein-of-seo-gene-family-forisome-functions-in-wound-sealing-and-act-as-biomimetic-smart-materials
#12
Vineet Kumar Srivastava, Narendra Tuteja
Forisomes protein belongs to SEO gene family and is unique to Fabaceae family. These proteins are located in sieve tubes of phloem and function to prevent loss of nutrient-rich photoassimilates, upon mechanical injury/wounding. Forisome protein is also known as ATP independent, mechanically active proteins. Despite the wealth of information role of forisome in plants are not yet fully understood. Recent reports suggest that forisomes protein can act as ideal model to study self assembly mechanism for development of nanotechnological devices like microfluidic system application in space exploration mission...
June 6, 2014: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/24805360/a-new-microfluidics-based-droplet-dispenser-for-icpms
#13
Pascal E Verboket, Olga Borovinskaya, Nicole Meyer, Detlef Günther, Petra S Dittrich
In this work, a novel droplet microfluidic sample introduction system for inductively coupled plasma mass spectrometry (ICPMS) is proposed and characterized. The cheap and disposable microfluidic chip generates droplets of an aqueous sample in a stream of perfluorohexane (PFH), which is also used to eject them as a liquid jet. The aqueous droplets remain intact during the ejection and can be transported into the ICP with >50% efficiency. The transport is realized via a custom-built system, which includes a membrane desolvator necessary for the PFH vapor removal...
June 17, 2014: Analytical Chemistry
https://www.readbyqxmd.com/read/24698846/the-development-and-application-of-high-throughput-cultivation-technology-in-bioprocess-development
#14
REVIEW
Quan Long, Xiuxia Liu, Yankun Yang, Lu Li, Linda Harvey, Brian McNeil, Zhonghu Bai
This review focuses on recent progress in the technology of high throughput (HTP) cultivation and its increasing application in quality by design (QbD) -driven bioprocess development. Several practical HTP strategies aimed at shortening process development (PD) timelines from DNA to large scale processes involving commercially available HTP technology platforms, including microtiter plate (MTP) culture, micro-scale bioreactors, and in parallel fermentation systems, etc., are critically reviewed in detail. This discussion focuses upon the relative strengths and weaknesses or limitations of each of these platforms in this context...
December 20, 2014: Journal of Biotechnology
https://www.readbyqxmd.com/read/24404050/fabrication-of-uniform-multi-compartment-particles-using-microfludic-electrospray-technology-for-cell-co-culture-studies
#15
Zhou Liu, Ho Cheung Shum
In this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments...
2013: Biomicrofluidics
https://www.readbyqxmd.com/read/24316463/an-integrated-microfludic-device-for-culturing-and-screening-of-giardia-lamblia
#16
Guo-Xia Zheng, Xue-Mei Zhang, Yu-Suo Yang, Shu-Rui Zeng, Jun-Feng Wei, Yun-Hua Wang, Ya-Jie Li
In vitro culturing of trophozoites was important for research of Giardia lamblia (G. lamblia), especially in discovery of anti-Giardia agents. The current culture methods mainly suffer from lab-intension or the obstacle in standardizing the gas condition. Thus, it could benefit from a more streamlined and integrated approach. Microfluidics offers a way to accomplish this goal. Here we presented an integrated microfluidic device for culturing and screening of G. lamblia. The device consisted of a polydimethylsiloxane (PDMS) microchip with an aerobic culture system...
February 2014: Experimental Parasitology
https://www.readbyqxmd.com/read/23301028/poly-dimethylsiloxane-pdms-affects-gene-expression-in-pc12-cells-differentiating-into-neuronal-like-cells
#17
Joanna M Łopacińska, Jenny Emnéus, Martin Dufva
INTRODUCTION: Microfluidics systems usually consist of materials like PMMA--poly(methyl methacrylate) and PDMS--poly(dimethylsiloxane) and not polystyrene (PS), which is usually used for cell culture. Cellular and molecular responses in cells grown on PS are well characterized due to decades of accumulated research. In contrast, the experience base is limited for materials used in microfludics chip fabrication. METHODS: The effect of different materials (PS, PMMA and perforated PMMA with a piece of PDMS underneath) on the growth and differentiation of PC12 (adrenal phaeochromocytoma) cells into neuronal-like cells was investigated using cell viability, cell cycle distribution, morphology, and gene expression analysis...
2013: PloS One
https://www.readbyqxmd.com/read/23282845/microfluidic-cell-trap-array-for-controlled-positioning-of-single-cells-on-adhesive-micropatterns
#18
Laiyi Lin, Yeh-Shiu Chu, Jean Paul Thiery, Chwee Teck Lim, Isabel Rodriguez
Adhesive micropattern arrays permit the continuous monitoring and systematic study of the behavior of spatially confined cells of well-defined shape and size in ordered configurations. This technique has contributed to defining mechanisms that control cell polarity and cell functions, including proliferation, apoptosis, differentiation and migration in two-dimensional cell culture systems. These micropattern studies often involve isolating a single cell on one adhesive protein micropattern using random seeding methods...
February 21, 2013: Lab on a Chip
https://www.readbyqxmd.com/read/23179465/analytical-study-of-a-microfludic-dna-amplification-chip-using-water-cooling-effect
#19
Jyh Jian Chen, Chia Ming Shen, Yu Wei Ko
A novel continuous-flow polymerase chain reaction (PCR) chip has been analyzed in our work. Two temperature zones are controlled by two external controllers and the other temperature zone at the chip center is controlled by the flow rate of the fluid inside a channel under the glass chip. By employing a water cooling channel at the chip center, the sequence of denaturation, annealing, and extension can be created due to the forced convection effect. The required annealing temperature of PCR less than 313 K can also be demonstrated in this chip...
April 2013: Biomedical Microdevices
https://www.readbyqxmd.com/read/22821484/surfactant-induced-electroosmotic-flow-in-microfluidic-capillaries
#20
Glareh Azadi, Anubhav Tripathi
Control of EOF in microfluidic devices is essential in applications such as protein/DNA sizing and high-throughput drug screening. With the growing popularity of poly(methyl methacrylate) (PMMA) as the substrate for polymeric-based microfludics, it is important to understand the effect of surfactants on EOF in these devices. In this article, we present an extensive investigation exploring changes in EOF rate induced by SDS, polyoxyethylene lauryl ether (Brij35) and CTAB in PMMA microfluidic capillaries. In a standard protein buffer (Tris-Glycine), PMMA capillaries exhibited a cathodic EOF with measured mobility of 1...
July 2012: Electrophoresis
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