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Na+K+ATPase

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https://www.readbyqxmd.com/read/29225109/raspberry-ketone-protects-against-isoproterenol-induced-myocardial-infarction-in-rats
#1
Vasim Khan, Sumit Sharma, Uma Bhandari, Syed Mansoor Ali, Syed Ehtaishamul Haque
AIM: The cardioprotective role of raspberry ketone (RK) against isoproterenol (ISO)-induced myocardial infarction (MI) in rats was assessed. MATERIALS AND METHODS: Rats were randomly divided into Group I - Vehicle control; Group II - Toxic control ISO (85mg/kg, s.c.); Group III, IV and V - RK (50, 100 and 200mg/kg, respectively) with ISO; Group VI- RK (200mg/kg) alone; Group VII - Propranolol (10mg/kg) with ISO; and Group VIII - Propranolol (10mg/kg) alone. After twenty-four hours of the last dose, animals were sacrificed and creatine kinase-MB, lactate dehydrogenase, total cholesterol, triglycerides, high-density-lipoprotein, low-density-lipoprotein, very-low-density-lipoprotein, malondialdehyde, reduced glutathione, superoxide dismutase, catalase, Na+, K+-ATPase, nitric oxide, histopathological and immunohistochemical analysis (tumor necrosis factor-α and inducible nitric oxide synthase) were performed...
December 7, 2017: Life Sciences
https://www.readbyqxmd.com/read/29225049/oxidative-stress-and-na-k-atpase-activity-differential-regulation-in-brainstem-and-forebrain-of-wistar-audiogenic-rats-may-lead-to-increased-seizure-susceptibility
#2
Gabriela Machado Parreira, Maria Daniela Aparecida Resende, Israel José Pereira Garcia, Daniela Bueno Sartori, Eduardo Henrique de Lima Umeoka, Lívea Dornela Godoy, Norberto Garcia-Cairasco, Leandro Augusto Barbosa, Hérica de Lima Santos, Cristiane Queixa Tilelli
The Wistar Audiogenic Rat (WAR) is a well-characterized seizure-prone, inbred rodent strain that, when acutely stimulated with high-intensity sounds, develops brainstem-dependent tonic-clonic seizures that can evolve to limbic-like, myoclonic (forebrain) seizures when the acoustic stimuli are presented chronically (audiogenic kindling). In order to investigate possible mechanisms underlying WAR susceptibility to seizures, we evaluated Na,K-ATPase activity, Ca-ATPase activity, Mg-ATPase activity, lipid membrane composition and oxidative stress markers in whole forebrain and whole brainstem samples of naïve WAR, as compared to samples from control Wistar rats...
December 7, 2017: Brain Research
https://www.readbyqxmd.com/read/29223875/snakes-exhibit-tissue-specific-variation-in-cardiotonic-steroid-sensitivity-of-na-k-atpase
#3
Shabnam Mohammadi, Georg Petschenka, Susannah S French, Akira Mori, Alan H Savitzky
Toads are among several groups of organisms chemically defended with lethal concentrations of cardiotonic steroids. As a result, most predators that prey on amphibians avoid toads. However, several species of snakes have gained resistance-conferring mutations of Na+/K+-ATPase, the molecular target of cardiotonic steroids, and can feed on toads readily. Despite recent advances in our understanding of this adaptation at the genetic level, we have lacked functional evidence for how mutations of Na+/K+-ATPase account for cardiotonic steroid resistance in snake tissues...
December 6, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29209224/na-h-exchanger-3-is-expressed-in-two-distinct-types-of-ionocyte-and-probably-augments-ammonia-excretion-in-one-of-them-in-the-gills-of-the-climbing-perch-exposed-to-seawater
#4
Xiu L Chen, Biyan Zhang, You R Chng, Jasmine L Y Ong, Shit F Chew, Wai P Wong, Siew H Lam, Yuen K Ip
The freshwater climbing perch, Anabas testudineus, is an euryhaline teleost and an obligate air-breather with the ability to actively excrete ammonia. Members of the Na+/H+ exchanger (NHE) family help maintain intracellular pH homeostasis and ionic balance through the electroneutral exchange of Na+ and H+. This study aimed to obtain, from the gills of A. testudineus, the full cDNA coding sequence of nhe3, and to determine the effects of exposure to seawater or 100 mmol l-1 of NH4Cl in fresh water on its mRNA and protein expression levels...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29209034/na-entry-through-heteromeric-trpc4-c1-channels-mediates-englerin-a-induced-cytotoxicity-in-synovial-sarcoma-cells
#5
Katsuhiko Muraki, Kaori Ohnishi, Akiho Takezawa, Hiroka Suzuki, Noriyuki Hatano, Yukiko Muraki, Nurasyikin Hamzah, Richard Foster, Herbert Waldmann, Peter Nussbaumer, Mathias Christmann, Robin S Bon, David J Beech
The sesquiterpene (-)Englerin A (EA) is an organic compound from the plant Phyllanthus engleri which acts via heteromeric TRPC4/C1 channels to cause cytotoxicity in some types of cancer cell but not normal cells. Here we identified selective cytotoxicity of EA in human synovial sarcoma cells (SW982 cells) and investigated the mechanism. EA induced cation channel current (Icat) in SW982 cells with biophysical characteristics of heteromeric TRPC4/C1 channels. Inhibitors of homomeric TRPC4 channels were weak inhibitors of the Icat and EA-induced cytotoxicity whereas a potent inhibitor of TRPC4/C1 channels (Pico145) strongly inhibited Icat and cytotoxicity...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208689/gestation-changes-sodium-pump-isoform-expression-leading-to-changes-in-ouabain-sensitivity-contractility-and-intracellular-calcium-in-rat-uterus
#6
Rachel V Floyd, Ali Mobasheri, Susan Wray
Developmental and tissue-specific differences in isoforms allow Na+, K+-ATPase function to be tightly regulated, as they control sensitivity to ions and inhibitors. Uterine contraction relies on the activity of the Na+, K+ATPase, which creates ionic gradients that drive excitation-contraction coupling. It is unknown whether Na+, K+ATPase isoforms are regulated throughout pregnancy or whether they have a direct role in modulating uterine contractility. We hypothesized that gestation-dependent differential expression of isoforms would affect contractile responses to Na+, K+ATPase α subunit inhibition with ouabain...
December 2017: Physiological Reports
https://www.readbyqxmd.com/read/29197217/rptec-tert1-cells-form-highly-differentiated-tubules-when-cultured-in-a-3d-matrix
#7
Philipp F Secker, Lisanne Luks, Nadja Schlichenmaier, Daniel R Dietrich
The proximal tubule is the primary site for renal solute reabsorption and secretion and thus a main target for drug-induced toxicity. Current nonclinical methods using 2D cell cultures are unable to fully recapitulate clinical drug responses mainly due to limited in vitro functional lifespan. Since extracellular matrices are known to be key regulators of cell development, culturing cells on classic 2D plastic surfaces inevitably results in loss of differentiation. Hence, 3D models of the human proximal tubule that recapitulate the in vivo morphology would allow for improved drug screening and disease modeling...
December 2, 2017: ALTEX
https://www.readbyqxmd.com/read/29192359/a-field-and-laboratory-study-of-the-responses-of-cytoprotection-and-osmoregulation-to-salinity-stress-in-mosquitofish-gambusia-affinis
#8
Jeng-Wei Tsai, Hon-Jung Liew, Jyun-Jiang Jhang, Shiou-Han Hung, Pei-Jie Meng, Ming-Yih Leu, Christopher Lim, Cheng-Hao Tang
The mosquitofish (Gambusia affinis) naturally inhabits freshwater (FW; 1-3‰) and seawater (SW; 28-33‰) ponds in constructed wetland. To explore the physiological status and molecular mechanisms for salinity adaptation of the mosquitofish, cytoprotective responses and osmoregulation were examined. In the field study, activation of protein quality control (PQC) mechanism through upregulation of the abundance of heat shock protein (HSP) 90 and 70 and ubiquitin-conjugated proteins was found in the mosquitofish gills from SW pond compared to the individuals of FW pond...
December 1, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29192345/transporter-mediated-interaction-between-platinum-drugs-and-sorafenib-at-the-cellular-level
#9
Verena Schneider, Selim Chaib, Claudia Spanier, Mandy Knapp, Violeta Moscvin, Laura Scordovillo, Alessandra Ewertz, Ulrich Jaehde, Ganna V Kalayda
Combining the multikinase inhibitor sorafenib with the platinum-based chemotherapy of solid tumors was expected to improve treatment outcome. However, in many clinical trials, no benefit from sorafenib addition to the platinum-containing regimen could be demonstrated. Moreover, in some studies, decreased survival of ovarian cancer patients as well as non-small cell lung cancer patients with squamous cell histology was observed. The aim of this study was to investigate the cellular mechanisms of the pharmacological interaction between platinum drugs and sorafenib in different cancer cell lines...
November 30, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29191836/steady-state-analysis-of-enzymes-with-non-michaelis-menten-kinetics-the-transport-mechanism-of-na-k-atpase
#10
José L E Monti, Mónica R Montes, Rolando C Rossi
Procedures to define kinetic mechanisms from catalytic activity measurements that obey the Michaelis-Menten equation are well-established. In contrast, analytical tools for enzymes displaying non-Michaelis-Menten kinetics are underdeveloped and transient-state measurements, when feasible, are therefore preferred in kinetic studies. Of note, transient-state determinations evaluate only partial reactions, and these might not participate in the reaction cycle. Here, we provide a general procedure to characterize kinetic mechanisms from steady-state determinations...
November 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29185184/changes-in-contractile-and-metabolic-parameters-of-skeletal-muscle-as-rats-age-from-3-to-12-months
#11
Hongyang Xu, Graham D Lamb, Robyn M Murphy
Laboratory rats are considered mature at 3 months despite that musculoskeletal growth is still occurring. Changes in muscle physiological and biochemical characteristics during development from 3 months, however, are not well understood. Whole muscles and single skinned fibres from fast-twitch extensor digitorum longus (EDL) and predominantly slow-twitch soleus (SOL) muscles were examined from male Sprague-Dawley rats (3, 6, 9, 12 months). Ca2+ sensitivity of contractile apparatus decreased with age in both fast- (~ 0...
November 28, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/29181400/effects-of-nitric-oxide-on-renal-proximal-tubular-na-transport
#12
REVIEW
Nobuhiko Satoh, Motonobu Nakamura, Atsushi Suzuki, Hiroyuki Tsukada, Shoko Horita, Masashi Suzuki, Kyoji Moriya, George Seki
Nitric oxide (NO) has a wide variety of physiological functions in the kidney. Besides the regulatory effects in intrarenal haemodynamics and glomerular microcirculation, in vivo studies reported the diuretic and natriuretic effects of NO. However, opposite results showing the stimulatory effect of NO on Na+ reabsorption in the proximal tubule led to an intense debate on its physiological roles. Animal studies have showed the biphasic effect of angiotensin II (Ang II) and the overall inhibitory effect of NO on the activity of proximal tubular Na+ transporters, the apical Na+/H+ exchanger isoform 3, basolateral Na+/K+ ATPase, and the Na+/HCO3- cotransporter...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29180359/tegoprazan-a-novel-potassium-competitive-acid-blocker-to-control-gastric-acid-secretion-and-motility
#13
Nobuyuki Takahashi, Yukinori Take
Tegoprazan, (S)-4-((5,7-difluorochroman-4-yl)oxy)-N,N,2-trimethyl-1H-benzo[d]imidazole-6-carboxamide, a potassium-competitive acid blocker (P-CAB), is a novel potent and highly selective inhibitor of gastric H+/K+-ATPase. Tegoprazan inhibited porcine, canine, and human H+/K+-ATPases in vitro with IC50 values ranging 0.29 ~ 0.52 μM while that for canine kidney Na+/K+-ATPase was more than 100 μM. A kinetic analysis revealed that tegoprazan inhibited H+/K+-ATPase with potassium-competitive manner and the binding was reversible...
November 27, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29176951/much-more-than-a-cardiotonic-steroid-modulation-of-inflammation-by-ouabain
#14
REVIEW
Luiz H A Cavalcante-Silva, Éssia de Almeida Lima, Deyse C M Carvalho, José M de Sales-Neto, Anne K de Abreu Alves, José G F M Galvão, Juliane S de França da Silva, Sandra Rodrigues-Mascarenhas
Since the discovery of ouabain as a cardiotonic steroid hormone present in higher mammals, research about it has progressed rapidly and several of its physiological and pharmacological effects have been described. Ouabain can behave as a stress hormone and adrenal cortex is its main source. Direct effects of ouabain are originated due to the binding to its receptor, the Na+/K+-ATPase, on target cells. This interaction can promote Na+ transport blockade or even activation of signaling transduction pathways (e...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29175347/adaptation-of-the-white-shrimp-litopenaeus-vannamei-to-gradual-changes-to-a-low-ph-environment
#15
Si-Yin Han, Bao-Jie Wang, Mei Liu, Meng-Qiang Wang, Ke-Yong Jiang, Xin-Wei Liu, Lei Wang
pH variation could cause a stress response in euryhaline penaeids, we evaluated the mortality, growth performance, osmoregulation gene expression, digestive enzyme activity, histology, and resistance against Vibrio parahemolyticus of white shrimp Litopenaeus vannamei reared under conditions of gradual changes to a low-pH environment (gradual-low pH, 6.65-8.20) or a high-pH environment (gradual-high pH, 8.20-9.81) versus a normal pH environment (8.14-8.31) during a 28-d experiment. Consequently, under gradual-high pH, the cumulative mortality rate (CMR) rose with time until 39...
November 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29174112/mechanism-of-rhinella-icterica-spix-1824-toad-poisoning-using-in-vitro-neurobiological-preparations
#16
Raquel Soares Oliveira, Allan Pinto Leal, Barbara Ogata, Carlos Gabriel Moreira de Almeida, Douglas Silva Dos Santos, Leandro Homrich Lorentz, Cleci Menezes Moreira, Karla de Castro Figueiredo Bordon, Eliane Candiani Arantes, Tiago Gomes Dos Santos, Cháriston André Dal Belo, Lúcia Vinadé
The biological activity of Rhinella icterica toxic secretion (RITS) was evaluated on chick neuromuscular junctions, rat heart́s tissue and mice hippocampal slices. At chick biventer cervicis preparation, RITS (5, 10 and 20μg/mL) produced a concentration-independent irreversible neuromuscular blockade, which was preceded by a transitory increase of muscle twitch tension with the lowest concentration, in 120minutes recordings. In this set of experiments, RITS incubation partially prevented the curare neuromuscular blockade...
November 23, 2017: Neurotoxicology
https://www.readbyqxmd.com/read/29172150/effects-of-ibuprofen-and-carbamazepine-on-the-ion-transport-system-and-fatty-acid-metabolism-of-temperature-conditioned-juveniles-of-solea-senegalensis
#17
A González-Mira, A Torreblanca, F Hontoria, J C Navarro, E Mañanós, I Varó
The increasing presence of pharmaceuticals in aquatic environments in the last decades, derived from human and veterinary use, has become an important environmental problem. Previous studies have shown that ibuprofen (IB) and carbamazepine (CBZ) modify physiological and biochemical processes in Senegalese sole (Solea senegalensis) in a temperature-dependent manner. In other vertebrates, there is evidence that both of these pharmaceuticals interfere with the 'arachidonic acid (AA) cascade', which is responsible for the biosynthesis of numerous enzymes that are involved in the osmoregulatory process...
November 21, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29169695/immunohistochemical-analysis-of-the-distribution-of-molecules-involved-in-ionic-and-ph-regulation-in-the-lancelet-branchiostoma-floridae-hubbs-1922
#18
Ivan Cuoghi, Clara Lazzaretti, Mauro Mandrioli, Lucrezia Mola, Aurora Pederzoli
The aim of present work is to analyse the distribution of carbonic anhydrase II (CAII), cystic fibrosis transmembrane regulator (CFTR), vacuolar-type H(+)-ATPase (V-H(+)-ATPase), Na(+)/K(+) ATPase, Na(+)/H(+) exchanger (NHE) and SLC26A6 (solute carrier family 26, member 6), also known as pendrin protein, in the lancelet Branchiostoma floridae in order to go in depth in the evolution of osmoregulation and pH regulation in Chordates. In view of their phylogenetic position, lancelets may indeed provide a critical point of reference for studies on osmoregulation evolution in Chordates...
November 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29169235/hypermagnesuria-in-humans-following-acute-intravenous-administration-of-digoxin
#19
Nabil Abu-Amer, Eldar Priel, Steven J.D. Karlish, Zvi Farfel, Haim Mayan
Background: Hypomagnesemia is a known predisposing condition for the appearance of digitalis toxicity. The detection of a genetic form of Mg urinary wasting with hypomagnesemia being caused by a mutation in the γ subunit (FXYD2) of the Na,K-ATPase, the pharmacological target of Digoxin, prompted us to investigate whether Digoxin administration increases urinary Mg excretion. Methods: Two groups of subjects, with rapid atrial fibrillation, received intravenous Digoxin (n = 9) or verapamil (n = 8), for heart rate control...
October 30, 2017: Nephron
https://www.readbyqxmd.com/read/29169161/digoxin-and-hypermagnesuria
#20
Luca Zanoli, Paolo Lentini, Pasquale Fatuzzo
In a recent issue of Nephron, Abu-Amer et al.[<xref ref-type="bibr" rid="ref1">1</xref>] reported the presence of hypermagnesuria in patients following acute intravenous administration of digoxin and suggested that the Na+/K+-ATPase γ-subunit, which is the pharmacological target of digoxin, can play a role in this process. Hypermagnesuria induced by digoxin may have important clinical consequences, particularly in the presence of inherited and acquired conditions associated with hypermagnesuria and hypomagnesemia...
November 23, 2017: Nephron
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