keyword
https://read.qxmd.com/read/38540017/morphometric-and-enzymatic-changes-in-gills-of-rainbow-trout-after-exposure-to-elevated-temperature-indications-for-gill-remodeling
#1
JOURNAL ARTICLE
Franz Lahnsteiner
Seven-month-old rainbow trout acclimated to 9 °C were used. The fish were gradually adapted to a water temperature of 20 °C over a period of seven days and then exposed to this temperature for 32 days. Changes in gill morphometry and histology and in enzyme activities in comparison to fish kept at 9 °C were investigated. No histopathological abnormalities were discerned at the heightened temperature. The gill epithelium thickened by approximately 40%, suggesting an increase in the branchial diffusion barrier for ions, water, and gases...
March 16, 2024: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/38518606/inhibition-the-ubiquitination-of-enac-and-na-k-atpase-with-erythropoietin-promotes-alveolar-fluid-clearance-in-sepsis-induced-acute-respiratory-distress-syndrome
#2
JOURNAL ARTICLE
Ye Gao, Fei Cao, Xinyi Tian, Qianping Zhang, Congcong Xu, Bowen Ji, Ye-An Zhang, Linan Du, Jun Han, Li Li, Siyu Zhou, Yuqiang Gong, Binyu Ying, Fang Gao-Smith, Shengwei Jin
Sepsis-induced acute respiratory distress syndrome (ARDS) causes significant fatalities worldwide and lacks pharmacological intervention. Alveolar fluid clearance (AFC) plays a pivotal role in the remission of ARDS and is markedly impaired in the pathogenesis of ARDS. Here, we demonstrated that erythropoietin could effectively ameliorate lung injury manifestations and lethality, restore lung function and promote AFC in a rat model of lipopolysaccharide (LPS)-induced ARDS. Moreover, it was proven that EPO-induced restoration of AFC occurs through triggering the total protein expression of ENaC and Na,K-ATPase channels, enhancing their protein abundance in the membrane, and suppressing their ubiquitination for degeneration...
March 21, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38517564/the-effect-of-cytochrome-c-on-na-k-atpase
#3
JOURNAL ARTICLE
Gvantsa Chkadua, Eka Nozadze, Leila Tsakadze, Lia Shioshvili, Nana Arutinova, Marine Leladze, Sopio Dzneladze, Maia Javakhishvili, Tamar Jariashvili, Elene Petriashvili
Na,K-ATPase is a crucial enzyme responsible for maintaining Na+ , K+ -gradients across the cell membrane, which is essential for numerous physiological processes within various organs and tissues. Due to its significance in cellular physiology, inhibiting Na,K-ATPase can have profound physiological consequences. This characteristic makes it a target for various pharmacological applications, and drugs that modulate the pump's activity are thus used in the treatment of several medical conditions. Cytochrome c (Cytc) is a protein with dual functions in the cell...
March 22, 2024: Journal of Bioenergetics and Biomembranes
https://read.qxmd.com/read/38513591/-keep-on-rockin-repurposed-rock-inhibitors-to-boost-corneal-endothelial-regeneration
#4
JOURNAL ARTICLE
Hendrik Vercammen, Martin Ondra, Jana Kotulova, Edgar Cardenas De La Hoz, Charissa Witters, Katerina Jecmenova, Maxim Le Compte, Christophe Deben, Sorcha Ní Dhubhghaill, Carina Koppen, Marián Hajdúch, Bert Van den Bogerd
The global shortage of corneal endothelial graft tissue necessitates the exploration of alternative therapeutic strategies. Rho-associated protein kinase inhibitors (ROCKi), recognized for their regenerative potential in cardiology, oncology, and neurology, have shown promise in corneal endothelial regeneration. This study investigates the repurposing potential of additional ROCKi compounds. Through screening a self-assembled library of ROCKi on B4G12 corneal endothelial cells, we evaluated their dose-dependent effects on proliferation, migration, and toxicity using live-cell imaging...
March 20, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38512049/single-cell-rna-sequencing-profiling-cellular-heterogeneity-and-specific-responses-of-fish-gills-to-microplastics-and-nanoplastics
#5
JOURNAL ARTICLE
Siwen Zheng, Wen-Xiong Wang
Fish gills are highly sensitive organs for microplastic (MP) and nanoplastic (NP) invasions, but the cellular heterogeneity of fish gills to MPs and NPs remains largely unknown. We employed single-cell RNA sequencing to investigate the responses of individual cell populations in tilapia Oreochromis niloticus gills to MP and NP exposure at an environmentally relevant concentration. Based on the detected differentially expressed gene (DEG) numbers, the most affected immune cells by MP exposure were macrophages, while the stimulus of NPs primarily targeted T cells...
March 21, 2024: Environmental Science & Technology
https://read.qxmd.com/read/38486306/bioprinting-of-human-pluripotent-stem-cell-derived-corneal-endothelial-cells-with-hydrazone-crosslinked-hyaluronic-acid-bioink
#6
JOURNAL ARTICLE
Pyry Grönroos, Anni Mörö, Paula Puistola, Karoliina Hopia, Maija Huuskonen, Taina Viheriälä, Tanja Ilmarinen, Heli Skottman
BACKGROUND: Human corneal endothelial cells lack regenerative capacity through cell division in vivo. Consequently, in the case of trauma or dystrophy, the only available treatment modality is corneal tissue or primary corneal endothelial cell transplantation from cadaveric donor which faces a high global shortage. Our ultimate goal is to use the state-of-the-art 3D-bioprint technology for automated production of human partial and full-thickness corneal tissues using human stem cells and functional bioinks...
March 14, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38485080/senolytics-prevent-age-associated-changes-in-female-mice-brain
#7
JOURNAL ARTICLE
Olivia Wyse Faria, Mayara Sandrielly Soares de Aguiar, Julia Eisenhardt de Mello, Fernando Lopez Alvez, Karina Pereira Luduvico, Driele Neske Garcia, Augusto Schneider, Michal M Masternak, Roselia Maria Spanevello, Francieli Moro Stefanello
PURPOSE: Considering that the combination of dasatinib and quercetin (D + Q) demonstrated a neuroprotective, as well as that females experience a decline in hormonal levels during aging and this is linked to increased susceptibility to Alzheimer's disease, in this study we evaluated the effect of D + Q on inflammatory and oxidative stress markers and on acetylcholinesterase and Na+ , K+ -ATPase activities in brain of female mice. METHODS: Female C57BL/6 mice were divided in Control and D (5 mg/kg) + Q (50 mg/kg) treated...
March 12, 2024: Neuroscience Letters
https://read.qxmd.com/read/38479999/brain-sodium-sensing-for-regulation-of-thirst-salt-appetite-and-blood-pressure
#8
REVIEW
Takeshi Y Hiyama
The brain possesses intricate mechanisms for monitoring sodium (Na) levels in body fluids. During prolonged dehydration, the brain detects variations in body fluids and produces sensations of thirst and aversions to salty tastes. At the core of these processes Nax , the brain's Na sensor, exists. Specialized neural nuclei, namely the subfornical organ (SFO) and organum vasculosum of the lamina terminalis (OVLT), which lack the blood-brain barrier, play pivotal roles. Within the glia enveloping the neurons in these regions, Nax collaborates with Na+ /K+ -ATPase and glycolytic enzymes to drive glycolysis in response to elevated Na levels...
March 2024: Physiological Reports
https://read.qxmd.com/read/38472560/pebp4-deficiency-aggravates-lps-induced-acute-lung-injury-and-alveolar-fluid-clearance-impairment-via-modulating-pi3k-akt-signaling-pathway
#9
JOURNAL ARTICLE
Qiao-Qing Shi, Yong-Hong Huang, Yu-Fei Li, Shuang-Yan Zhen, Yan-Hong Li, Jia-Yi Huang, Jia-Yang Wang, Xiao-Yan Zhou
Acute lung injury (ALI) is a common clinical syndrome, which often results in pulmonary edema and respiratory distress. It has been recently reported that phosphatidylethanolamine binding protein 4 (PEBP4), a basic cytoplasmic protein, has anti-inflammatory and hepatoprotective effects, but its relationship with ALI remains undefined so far. In this study, we generated PEBP4 knockout (KO) mice to investigate the potential function of PEBP4, as well as to evaluate the capacity of alveolar fluid clearance (AFC) and the activity of phosphatidylinositide 3-kinases (PI3K)/serine-theronine protein kinase B (PKB, also known as AKT) signaling pathway in lipopolysaccharide (LPS)-induced ALI mice models...
March 13, 2024: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/38466286/substrate-stiffness-modulates-stemness-and-differentiation-of-rabbit-corneal-endothelium-through-the-paxillin-yap-pathway
#10
JOURNAL ARTICLE
Shuting Liu, Hua Chen, Huatao Xie, Xin Liu, Mingchang Zhang
PURPOSE: To explore the role of substrate stiffness and the mechanism beneath corneal endothelial cells' (CECs') stemness maintenance and differentiation. METHODS: CECs were divided into central zone (8 mm trephined boundary) and peripheral zone (8 mm trephined edge with attached limbal). Two zones were analyzed by hematoxylin-eosin staining and scanning electron microscopy for anatomic structure. The elastic modulus of Descemet's membrane (DM) was analyzed by atomic force microscopy...
March 5, 2024: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/38465401/a-modeling-analysis-of-whole-body-potassium-regulation-on-a-high-potassium-diet-proximal-tubule-and-tubuloglomerular-feedback-effects
#11
JOURNAL ARTICLE
Melissa M Stadt, Anita T Layton
Potassium (K+ ) is an essential electrolyte that plays a key role in many physiological process, including mineralcorticoid action, systemic blood-pressure regulation, as well as hormone secretion and action. Indeed, maintaining K+ balance is critical for normal cell function, as too high or too low K+ levels can have serious and potentially deadly health consequences. K+ homeostasis is achieved by an intricate balance between the intracellular and extracellular fluid as well as balance between K+ intake and excretion...
March 11, 2024: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://read.qxmd.com/read/38464201/protein-carbonylation-causes-sarcoplasmic-reticulum-ca2-overload-by-increasing-intracellular-na-level-in-ventricular-myocytes
#12
Elisa Bovo, Jaroslava Seflova, Seth L Robia, Aleksey V Zima
Diabetes is commonly associated with an elevated level of reactive carbonyl species due to alteration of glucose and fatty acid metabolism. These metabolic changes cause an abnormality in cardiac Ca 2+ regulation that can lead to cardiomyopathies. In this study, we explored how the reactive α-dicarbonyl methylglyoxal (MGO) affects Ca 2+ regulation in mouse ventricular myocytes. Analysis of intracellular Ca 2+ dynamics revealed that MGO (200 µM) increases action potential (AP)-induced Ca 2+ transients and sarcoplasmic reticulum (SR) Ca 2+ load, with a limited effect on L - type Ca 2+ channel-mediated Ca 2+ transients and SERCA - mediated Ca 2+ uptake...
March 1, 2024: Research Square
https://read.qxmd.com/read/38452524/aging-effects-of-titanium-dioxide-on-cu-toxicity-to-daphnia-magna-exploring-molecular-docking-and-significance-of-surface-properties
#13
JOURNAL ARTICLE
Yao Li, Wenhong Fan, Xiaolong Yang, Shu Liu, Ying Wang, Wen-Xiong Wang
Cosmetics and personal care products containing titanium dioxide nanoparticles (TiO2 NPs) may enter aquatic environments, where the surface coatings of TiO2 NPs may change with aging due to environmental factors such as light, and potentially affect their bioaccumulation and toxicity. This study examined how aging impacted the physicochemical properties of three commercially available TiO2 NPs and subsequent influence on the bioaccumulation and toxicity of copper (Cu) in Daphnia magna (D. magna). We demonstrated that aging significantly affected the hydrophobicity of TiO2 NPs, which affected their binding to water molecules and adsorption of Cu...
February 29, 2024: Water Research
https://read.qxmd.com/read/38451282/cardenolide-glycosides-sensitize-gefitinib-induced-apoptosis-in-non-small-cell-lung-cancer-inhibition-of-na-k-atpase-serving-as-a-switch-on-mechanism
#14
JOURNAL ARTICLE
Chi-Min Du, Wohn-Jenn Leu, Yi-Huei Jiang, She-Hung Chan, Ih-Sheng Chen, Hsun-Shuo Chang, Lih-Ching Hsu, Jui-Ling Hsu, Jih-Hwa Guh
The treatment of non-small cell lung cancer (NSCLC) is known as a significant level of unmet medical need in spite of the progress in targeted therapy and personalized therapy. Overexpression of the Na+ /K+ -ATPase contributes to NSCLC progression, suggesting its potentiality in antineoplastic approaches. Epi-reevesioside F, purified from Reevesia formosana, showed potent anti-NSCLC activity through inhibiting the Na+ /K+ -ATPase, leading to internalization of α1- and α3-subunits in Na+ /K+ -ATPase and suppression of Akt-independent mTOR-p70S6K-4EBP1 axis...
March 7, 2024: Naunyn-Schmiedeberg's Archives of Pharmacology
https://read.qxmd.com/read/38449343/effect-of-citric-acid-on-cell-membrane-structure-and-function-of-issatchenkia-terricola-wjl-g4
#15
JOURNAL ARTICLE
Xiangfeng Meng, Xinyi Liu, Yihong Bao, Ting Luo, Jinling Wang
AIMS: This study aimed to investigate the changes of cell membrane structure and function of Issatchenkia terricola under citric acid by performing physiological analysis. METHODS AND RESULTS: The membrane integrity, surface hydrophobicity, structure, fluidity, apoptosis and fatty acid methyl esters composition of I. terricola WJL-G4 cells were determined by propidium iodide staining, microbial adhesion to hydrocarbon test, transmission electron microscopy analysis, fluorescence anisotropy, flow cytometry and gas chromatography-mass, respectively...
March 6, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38438092/the-role-of-the-antennal-glands-and-gills-in-acid-base-regulation-and-ammonia-excretion-of-a-marine-osmoconforming-brachyuran
#16
JOURNAL ARTICLE
Garett Joseph Patrick Allen, Alex R Quijada-Rodriguez, Jonathan M Wilson, Dirk Weihrauch
The excretory mechanisms of stenohaline marine osmoconforming crabs are often compared to those of the more extensively characterized euryhaline osmoregulating crabs. These comparisons may have limitations, given that unlike euryhaline brachyurans the gills of stenohaline marine osmoconformers possess ion-leaky paracellular pathways and lack the capacity to undergo ultrastructural changes that can promote ion-transport processes in dilute media. Furthermore, the antennal glands of stenohaline marine osmoconformers are poorly characterized making it difficult to determine what role urinary processes play in excretion...
March 2, 2024: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://read.qxmd.com/read/38436710/bioinformatic-study-of-possible-acute-regulation-of-acid-secretion-in-the-stomach
#17
JOURNAL ARTICLE
Yan Hay Grace Lee, Nicole T Cerf, Nicholas Shalaby, Mónica R Montes, Ronald J Clarke
The gastric H+ ,K+ -ATPase is an integral membrane protein which derives energy from the hydrolysis of ATP to transport H+ ions from the parietal cells of the gastric mucosa into the stomach in exchange for K+ ions. It is responsible for the acidic environment of the stomach, which is essential for digestion. Acid secretion is regulated by the recruitment of the H+ ,K+ -ATPase from intracellular stores into the plasma membrane on the ingestion of food. The similar amino acid sequences of the lysine-rich N-termini α-subunits of the H+ ,K+ - and Na+ ,K+ -ATPases, suggests similar acute regulation mechanisms, specifically, an electrostatic switch mechanism involving an interaction of the N-terminal tail with the surface of the surrounding membrane and a modulation of the interaction via regulatory phosphorylation by protein kinases...
March 4, 2024: Journal of Membrane Biology
https://read.qxmd.com/read/38435539/the-oxidative-status-and-na-k-atpase-activity-in-obsessive-compulsive-disorder-a-case-control-study
#18
JOURNAL ARTICLE
Amir Hossein Mohammadi, Ebrahim Balandeh, Jila Hasani, Mohammad Karimian, Vajiheh Arabshahi, Morteza Pourfarzam, Fereshteh Bahmani, Gholamreza Namazi
BACKGROUND: Oxidative stress is involved in pathogenesis of some psychiatric disorders. To examine the role of oxidative stress in the etiopathogenesis of obsessive-compulsive disorder (OCD), we aimed to determine oxidative stress indices, including malondialdehyde (MDA) levels in serum and red blood cells (RBC) membrane, total antioxidant capacity (TAC), serum glutathione (GSH) levels, serum antioxidant vitamins (A and E), and Na+ /K+ -ATPase activity, in patients with the mentioned disorder vs...
2024: BioMed Research International
https://read.qxmd.com/read/38434572/fabrication-of-bioengineered-corneal-endothelial-grafts-using-an-allogeneic-cornea-derived-matrix
#19
REVIEW
Lijie Xie, Xiaojuan Dong, Jianping Ji, Chen Ouyang, Jing Wu, Chao Hou, Ting Huang
Corneal endothelial keratoplasty has been the primary treatment method of endothelial decompensation, but it is often limited in clinical practice due to global shortage of donor cornea. Here, we explored using an ultra-thin allogeneic cornea-derived matrix (uACM) films as a substrate for constructing bioengineered corneal endothelial grafts. We evaluated the films' optical, mechanical, and structural properties, and measured the composition of the extracellular matrix. The uACM was an ultrathin and curved cornea-shaped film with favorable optical and mechanical properties...
April 2024: Materials today. Bio
https://read.qxmd.com/read/38431563/circadian-rhythm-of-intraocular-pressure
#20
REVIEW
Keisuke Ikegami
Intraocular pressure (IOP) plays a crucial role in glaucoma development, involving the dynamics of aqueous humor (AH). AH flows in from the ciliary body and exits through the trabecular meshwork (TM). IOP follows a circadian rhythm synchronized with the suprachiasmatic nucleus (SCN), the circadian pacemaker. The SCN resets peripheral clocks through sympathetic nerves or adrenal glucocorticoids (GCs). IOP's circadian rhythm is governed by circadian time signals, sympathetic noradrenaline (NE), and GCs, rather than the local clock...
March 2, 2024: Journal of Physiological Sciences: JPS
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