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Drosophila mirna

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https://www.readbyqxmd.com/read/29688430/regulation-of-large-number-of-weak-targets-new-insights-from-twin-micrornas
#1
Yixin Zhao, Pei Lin, Zhongqi Liufu, Hao Yang, Yang Lyu, Xu Shen, Chung-I Wu, Tian Tang
Each animal microRNA (miRNA) targets many genes for repression. Down-regulation of most of these targets is weak and has no detectable individual phenotypic effect. Whether this extensive weak repression is biologically relevant is a central issue in the debate on miRNA functionality. In the "small (target) pool" view, weak repression is non-functional and should be gradually removed during evolution. However, since the selective advantage of removing individual targets is small, testing this hypothesis is a challenge...
April 23, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29676724/wolbachia-mediated-protection-of-drosophila-melanogaster-against-systemic-infection-with-its-natural-viral-pathogen-drosophila-c-virus-does-not-involve-changes-in-levels-of-highly-abundant-mirnas
#2
Verna Monsanto-Hearne, Karyn N Johnson
The presence of Wolbachia confers virus protection to insects. The molecular mechanism underlying Wolbachia-mediated protection in this tripartite host-endosymbiont-virus interaction is not yet fully understood. In the bipartite association between Drosophila melanogaster and Drosophila C virus (DCV), changes in the expression of microRNAs (miRNAs) influence the outcome of viral pathogenesis. Here we examined whether changes in miRNA expression are similarly involved in the Drosophila-Wolbachia-DCV association...
April 20, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29663653/the-mir-7-and-bag-of-marbles-genes-regulate-hedgehog-pathway-signaling-in-blood-cell-progenitors-in-drosophila-larval-lymph-glands
#3
Tsuyoshi Tokusumi, Yumiko Tokusumi, Robert A Schulz
Hedgehog (Hh) pathway signaling is crucial for the maintenance of blood cell progenitors in the lymph gland hematopoietic organ present in Drosophila third instar larvae. Previous studies from our lab have likewise shown the importance of the mir-7 and bag of marbles (bam) genes in maintaining the progenitor state. Thus we sought to investigate a possible interaction between the Hh pathway and mir-7/bam in the prohemocyte population within this hematopoietic tissue. Gain of function mir-7 was able to rescue a blood cell progenitor depletion phenotype caused by Patched (Ptc) inhibition of Hh pathway signaling in these cells...
April 16, 2018: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/29550490/loquacious-pd-removes-phosphate-inhibition-of-dicer-2-processing-of-hairpin-rnas-into-sirnas
#4
Ryuya Fukunaga
Drosophila Dicer-2 processes RNA substrates into short interfering RNAs (siRNAs). Loquacious-PD (Loqs-PD), a dsRNA-binding protein that associates with Dicer-2, is required for processing of a subset of RNA substrates including hairpin RNAs into siRNAs. Inorganic phosphate-a small molecule present in all cell types-inhibits Dicer-2 from processing precursor of microRNAs (pre-miRNAs), which are processed by Dicer-1. Whether or how Loqs-PD modulates the inhibitory effect of inorganic phosphate on Dicer-2 processing of RNA substrates is unknown...
March 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29543534/ecdysone-responsive-microrna-252-5p-controls-the-cell-cycle-by-targeting-abi-in-drosophila
#5
Do-Hwan Lim, Seungjae Lee, Jee Yun Han, Min-Seok Choi, Jae-Sang Hong, Youngmo Seong, Young-Soo Kwon, Young Sik Lee
The steroid hormone ecdysone has a central role in the developmental transitions of insects through its control of responsive protein-coding and microRNA (miRNA) gene expression. However, the complete regulatory network controlling the expression of these genes remains to be elucidated. In this study, we performed cross-linking immunoprecipitation coupled with deep sequencing of endogenous Argonaute 1 (Ago1) protein, the core effector of the miRNA pathway, in Drosophila S2 cells. We found that regulatory interactions between miRNAs and their cognate targets were substantially altered by Ago1 in response to ecdysone signaling...
March 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29540498/the-mir-279-996-cluster-represses-receptor-tyrosine-kinase-signaling-to-determine-cell-fates-in-the-drosophila-eye
#6
Hong Duan, Luis F de Navas, Fuqu Hu, Kailiang Sun, Yannis E Mavromatakis, Kayla Viets, Cyrus Zhou, Joshua Kavaler, Robert J Johnston, Andrew Tomlinson, Eric C Lai
Photoreceptors in the crystalline Drosophila eye are recruited by receptor tyrosine kinase (RTK)/Ras signaling, mediated by the Epidermal Growth Factor receptor (EGFR) and Sevenless receptor. Analyses of an allelic deletion series of the mir-279/996 locus, along with a panel of modified genomic rescue transgenes, show that normal Drosophila eye patterning depends on both miRNAs. Transcriptional reporter and activity sensor transgenes reveal expression and function of miR-279/996 in non-neural cells of the developing eye...
March 14, 2018: Development
https://www.readbyqxmd.com/read/29519294/hippo-signaling-special-issue-of-bmb-reports-in-2018
#7
Eek-Hoon Jho
Since the first component of Hippo signaling, Wts in Drosophila, was identified in 1995, the progress of Hippo signaling studies has been very slow initially. However, after the findings suggesting that the core kinase pathway established in Drosophila was evolutionarily conserved in metazoans for the determination of organ size around 2008, the number of publications related to Hippo signaling has grown exponentially. Identification of molecular mechanisms underlying Hippo signaling response to intrinsic cues, such as cell-cell contact and mechanotransduction, as well as extrinsic cues, such as nutrients and soluble factors, has been one of the key topics of Hippo signaling...
March 9, 2018: BMB Reports
https://www.readbyqxmd.com/read/29487148/regulation-of-circadian-behavior-by-astroglial-micrornas-in-drosophila
#8
Samantha You, Tudor A Fulga, David Van Vactor, F Rob Jackson
We describe a genome-wide microRNA (miRNA)-based screen to identify brain glial cell functions required for circadian behavior. To identify glial miRNAs that regulate circadian rhythmicity, we employed a collection of "miR-sponges" to inhibit miRNA function in a glia-specific manner. Our initial screen identified 20 glial miRNAs that regulate circadian behavior. We studied two miRNAs, miR-263b and miR-274, in detail and found that both function in adult astrocytes to regulate behavior. Astrocyte-specific inhibition of miR-263b or miR-274 in adults acutely impairs circadian locomotor activity rhythms with no effect on glial or clock neuronal cell viability...
March 2018: Genetics
https://www.readbyqxmd.com/read/29481550/cell-type-specific-sequencing-of-micrornas-from-complex-animal-tissues
#9
Chiara Alberti, Raphael A Manzenreither, Ivica Sowemimo, Thomas R Burkard, Jingkui Wang, Katharina Mahofsky, Stefan L Ameres, Luisa Cochella
MicroRNAs (miRNAs) play an essential role in the post-transcriptional regulation of animal development and physiology. However, in vivo studies aimed at linking miRNA function to the biology of distinct cell types within complex tissues remain challenging, partly because in vivo miRNA-profiling methods lack cellular resolution. We report microRNome by methylation-dependent sequencing (mime-seq), an in vivo enzymatic small-RNA-tagging approach that enables high-throughput sequencing of tissue- and cell-type-specific miRNAs in animals...
February 26, 2018: Nature Methods
https://www.readbyqxmd.com/read/29474379/small-rna-populations-revealed-by-blocking-rrna-fragments-in-drosophila-melanogaster-reproductive-tissues
#10
Emily K Fowler, Irina Mohorianu, Damian T Smith, Tamas Dalmay, Tracey Chapman
RNA interference (RNAi) is a complex and highly conserved regulatory mechanism mediated via small RNAs (sRNAs). Recent technical advances in high throughput sequencing have enabled an increasingly detailed analysis of sRNA abundances and profiles in specific body parts and tissues. This enables investigations of the localized roles of microRNAs (miRNAs) and small interfering RNAs (siRNAs). However, variation in the proportions of non-coding RNAs in the samples being compared can hinder these analyses. Specific tissues may vary significantly in the proportions of fragments of longer non-coding RNAs (such as ribosomal RNA or transfer RNA) present, potentially reflecting tissue-specific differences in biological functions...
2018: PloS One
https://www.readbyqxmd.com/read/29435938/transgene-like-animal-models-using-intronic-micrornas
#11
Shi-Lung Lin, Shin-Ju E Chang, Shao-Yao Ying
Transgenic animal models are valuable tools for testing gene functions and drug mechanisms in vivo. They are also the best similitude for a human body for etiological and pathological research of diseases. All pharmaceutically developed medicines must be proven to be safe and effective in animals before approval by the Food and Drug Administration (FDA) to be used in clinical trials. To this end, the transgenic animal models of diseases serve as the front line of drug evaluation. However, there is currently no transgenic animal model for microRNA (miRNA)-related research...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29428901/fragile-x-mental-retardation-protein-participates-in-non-coding-rna-pathways
#12
En-Hui Li, Xin Zhao, Ce Zhang, Wei Liu
Fragile X syndrome is one of the most common forms of inherited intellectual disability. It is caused by mutations of the Fragile X mental retardation 1(FMR1) gene, resulting in either the loss or abnormal expression of the Fragile X mental retardation protein (FMRP). Recent research showed that FMRP participates in non-coding RNA pathways and plays various important roles in physiology, thereby extending our knowledge of the pathogenesis of the Fragile X syndrome. Initial studies showed that the Drosophila FMRP participates in siRNA and miRNA pathways by interacting with Dicer, Ago1 and Ago2, involved in neural activity and the fate determination of the germline stem cells...
February 20, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29386086/up-regulation-of-mir-324-5p-inhibits-proliferation-and-invasion-of-colorectal-cancer-cells-by-targeting-elavl1
#13
Chijiang Gu, Mingyuan Zhang, Weiliang Sun, Changzheng Dong
Colorectal cancer (CRC) is a common clinical cancer, which remains incurable in most cases. MiRNAs are reported to playa part in development of various tumors. In the present study, we found that miR-324-5p was down-regulated in colorectal cancer cells while ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like Protein 1 (ELAVL1) showed a higher expression. MiR-324-5p transfection significantly inhibited the proliferation as well as invasion in both SW620 and SW480 cells. MiR-324-5p mimics transfection markedly decreased the expression of ELAVL1...
January 31, 2018: Oncology Research
https://www.readbyqxmd.com/read/29373753/dicer-partner-protein-tunes-the-length-of-mirnas-using-base-mismatch-in-the-pre-mirna-stem
#14
Li Zhu, Suresh K Kandasamy, Ryuya Fukunaga
Dicer partner proteins Drosophila Loquacious-PB (Loqs-PB) and human TRBP tune the length of miRNAs produced by Dicer from a subset of pre-miRNAs and thereby alter their target repertoire, by an unknown mechanism. Here, we developed a novel high-throughput method that we named Dram-seq (Dice randomized pre-miRNA pool and seq) to study length distributions of miRNAs produced from thousands of different pre-miRNA variants. Using Dram-seq, we found that a base-mismatch in the pre-miRNA stem can alter the length of miRNAs compared with a base-pair at the same position in both Drosophila and human, and is important for the miRNA length tuning by Loqs-PB...
January 24, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29361610/-mir-9a-mediates-the-role-of-lethal-giant-larvae-as-an-epithelial-growth-inhibitor-in-drosophila
#15
Scott G Daniel, Atlantis D Russ, Kathryn M Guthridge, Ammad I Raina, Patricia S Estes, Linda M Parsons, Helena E Richardson, Joyce A Schroeder, Daniela C Zarnescu
Drosophila lethal giant larvae ( lgl ) encodes a conserved tumor suppressor with established roles in cell polarity, asymmetric division, and proliferation control. Lgl's human orthologs, HUGL1 and HUGL2, are altered in human cancers, however, its mechanistic role as a tumor suppressor remains poorly understood. Based on a previously established connection between Lgl and Fragile X protein (FMRP), a miRNA-associated translational regulator, we hypothesized that Lgl may exert its role as a tumor suppressor by interacting with the miRNA pathway...
January 26, 2018: Biology Open
https://www.readbyqxmd.com/read/29358748/stress-dependent-mir-980-regulation-of-rbfox1-a2bp1-promotes-ribonucleoprotein-granule-formation-and-cell-survival
#16
Mariya M Kucherenko, Halyna R Shcherbata
Upon stress, profound post-transcriptional adjustments of gene expression occur in spatially restricted, subcellular, membraneless compartments, or ribonucleoprotein (RNP) granules, which are formed by liquid phase separation of RNA-binding proteins with low complexity sequence domains (LCDs). Here, we show that Rbfox1 is an LCD-containing protein that aggregates into liquid droplets and amyloid-like fibers and promiscuously joins different nuclear and cytoplasmic RNP granules. Using Drosophila oogenesis as an in vivo system for stress response, we demonstrate a mechanism by which Rbfox1 promotes cell survival...
January 22, 2018: Nature Communications
https://www.readbyqxmd.com/read/29345184/temperature-dependent-small-rna-expression-in-drosophila-melanogaster
#17
Isabel Fast, David Rosenkranz
Temperature has a major impact on gene expression in ectotherms. But until recently, it was not clear in which way, if any, small non-coding RNAs such as miRNAs or piRNAs contribute to thermosensitive gene regulation. We have recently shown that temperature-responsive miRNAs in Drosophila drive adaptation to different ambient temperatures on the transcriptome level. Moreover, we demonstrated that higher temperatures lead to a more efficient piRNA-dependent transposon silencing, possibly due to heat-induced unfolding of RNA secondary structures...
January 18, 2018: RNA Biology
https://www.readbyqxmd.com/read/29338692/emerging-roles-for-microrna-in-the-regulation-of-drosophila-circadian-clock
#18
REVIEW
Yongbo Xue, Yong Zhang
BACKGROUND: The circadian clock, which operates within an approximately 24-h period, is closely linked to the survival and fitness of almost all living organisms. The circadian clock is generated through a negative transcription-translation feedback loop. microRNAs (miRNAs) are small non-coding RNAs comprised of approximately 22 nucleotides that post-transcriptionally regulate target mRNA by either inducing mRNA degradation or inhibiting translation. RESULTS: In recent years, miRNAs have been found to play important roles in the regulation of the circadian clock, especially in Drosophila...
January 16, 2018: BMC Neuroscience
https://www.readbyqxmd.com/read/29314553/identification-of-mir-305-a-microrna-that-promotes-aging-and-its-target-mrnas-in-drosophila
#19
Makiko Ueda, Tetsuya Sato, Yasuyuki Ohkawa, Yoshihiro H Inoue
MicroRNAs (miRNAs) are involved in the regulation of important biological processes. Here, we describe a novel Drosophila miRNAs involved in aging. We selected eight Drosophila miRNAs, displaying high homology with seed sequences of aging-related miRNAs characterized in other species, and investigated whether the over-expression of these miRNAs affected aging in Drosophila adult flies. The lifespan of adults over-expressing miR-305, a miRNA showing high homology with miR-239 in C. elegans, was significantly shorter...
February 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29312571/systematic-analysis-reveals-tumor-enhancing-and-suppressing-micrornas-in-drosophila-epithelial-tumors
#20
Zhiqiang Shu, Yi-Chun Huang, William H Palmer, Yoichiro Tamori, Gengqiang Xie, Hui Wang, Nan Liu, Wu-Min Deng
Despite their emergence as an important class of noncoding RNAs involved in cancer cell transformation, invasion, and migration, the precise role of microRNAs (miRNAs) in tumorigenesis remains elusive. To gain insights into how miRNAs contribute to primary tumor formation, we conducted an RNA sequencing (RNA-Seq) analysis of Drosophila wing disc epithelial tumors induced by knockdown of a neoplastic tumor-suppressor gene (nTSG) lethal giant larvae ( lgl ), combined with overexpression of an active form of oncogene Ras ( Ras V12 ), and identified 51 mature miRNAs that changed significantly in tumorous discs...
December 12, 2017: Oncotarget
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